CN106038623A - A preparing method for valeriana officinalis var. latifolia root volatile oil and inflammation-diminishing and pain-easing applications of the volatile oil - Google Patents

A preparing method for valeriana officinalis var. latifolia root volatile oil and inflammation-diminishing and pain-easing applications of the volatile oil Download PDF

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CN106038623A
CN106038623A CN201610302999.4A CN201610302999A CN106038623A CN 106038623 A CN106038623 A CN 106038623A CN 201610302999 A CN201610302999 A CN 201610302999A CN 106038623 A CN106038623 A CN 106038623A
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volatile oil
valeriana officinalis
officinalis var
latifolia
latifolia root
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杨成东
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Nanjing Zelang Medical Technology Co Ltd
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Nanjing Zelang Medical Technology Co Ltd
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
    • A61K36/18—Magnoliophyta (angiosperms)
    • A61K36/185—Magnoliopsida (dicotyledons)
    • C—CHEMISTRY; METALLURGY
    • C11—ANIMAL OR VEGETABLE OILS, FATS, FATTY SUBSTANCES OR WAXES; FATTY ACIDS THEREFROM; DETERGENTS; CANDLES
    • C11B—PRODUCING, e.g. BY PRESSING RAW MATERIALS OR BY EXTRACTION FROM WASTE MATERIALS, REFINING OR PRESERVING FATS, FATTY SUBSTANCES, e.g. LANOLIN, FATTY OILS OR WAXES; ESSENTIAL OILS; PERFUMES
    • C11B9/00—Essential oils; Perfumes
    • C11B9/02—Recovery or refining of essential oils from raw materials
    • C11B9/025—Recovery by solvent extraction
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/10—Preparation or pretreatment of starting material
    • A61K2236/19—Preparation or pretreatment of starting material involving fermentation using yeast, bacteria or both; enzymatic treatment
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/30—Extraction of the material
    • A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
    • A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
    • A61K2236/50—Methods involving additional extraction steps
    • A61K2236/55—Liquid-liquid separation; Phase separation

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  • Health & Medical Sciences (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Alternative & Traditional Medicine (AREA)
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Abstract

The invention relates to a preparing method for valeriana officinalis var. latifolia root volatile oil and inflammation-diminishing and pain-easing applications of the volatile oil. The method includes crushing valeriana officinalis var. latifolia root, sieving with a sieve having a size of 20 meshes, dissolving cellulase the weight of which is 1-3% of the weight of the raw material into an acetic acid/sodium acetate buffer solution the pH of which is 4.0-5.0 and the weight of which is 0.5-1 time of the weight of the raw material, fully mixing, spraying the valeriana officinalis var. latifolia root powder with the prepared buffer solution, sealing, covering with film, fermenting at 40-50 DEG C for 5-7 d, adding the fermented raw material into an ultrasonic device the frequency of which is 40 KHz, extracting for 2-3 h, filtering, and subjecting the extract liquid to vacuum concentration, extraction with an organic solvent, drying with anhydrous sodium sulfate, vacuum concentration, and other steps to obtain the volatile oil. Pharmacodynamic evaluation shows that the volatile oil has obvious inflammation-diminishing and pain-easing effects and has a wide application prospect.

Description

The preparation method of a kind of Valeriana officinalis var. latifolia root volatile oil and anti-inflammatory analgesic application thereof
Technical field
The invention belongs to pharmaceutical technology field, be specifically related to preparation method and the antiinflammatory thereof of a kind of Valeriana officinalis var. latifolia root volatile oil Analgesia application.
Background technology
Volatile oil is that a class has medicative active component, is the general name of the volatile ingredient extracted from Chinese medicine. Complex chemical composition, majority has aromatic odor, has many medical functions clinically.Through consulting literatures, it is known that The main pharmacological of volatile oil Chinese medicine is: (1) effect to central nervous system, essentially consist in analgesia, calm, antipyretic, Antiinflammatory etc.；(2) effect to cardiovascular system；(3) effect to respiratory system；(4) to digestion systemic effect；(5) antibacterial anti- Virus function；(6) antitumor action.
Valeriana officinalis var. latifolia root is the dry root of Valerianaceae valerian Valeriana officinalis var. latifolia, has tranquilizing mind, wind-damp dispelling, qi and blood circulation promotion With effects such as pain relievings, cure mainly the diseases such as irritability, palpitation and insomnia, rheumatic arthralgia, abdominal distention and traumatic injury.Valeriana officinalis var. latifolia root Volatile oil, based on terpene compound, contains alkene, alcohol, ketone, ester and ether compound respectively.Wherein borneol acetate, XIANGSHU Alkene and camphene are main component, and the content of borneol acetate is 37.95%.Terpene compound has hemostasis, antiinflammatory, pain relieving, disappears The multiple efficacies such as swollen, stomach invigorating, heat-clearing and toxic substances removing, the pharmacological action of pinene therein, borneol acetate etc. has already been confirmed.Thus Visible, the chemical composition that volatile oil comprises has powerful biological activity, has huge potential medical value.
Summary of the invention
It is an object of the invention to provide the preparation method and applications of a kind of Valeriana officinalis var. latifolia root volatile oil, this volatile oil is steady Qualitative good, bioactive ingredients content height, and anti-inflammatory analgesic effect is obvious.
For achieving the above object, the present invention is achieved by following technical solution:
A. Valeriana officinalis var. latifolia root is pulverized, cross 20 mesh sieves；
B. the cellulase of raw material weight 1 ~ 3% is dissolved in the acetic acid/sodium acetate of pH4.0 ~ 5.0 of raw material weight 0.5 ~ 1 times In buffer solution, mix homogeneously；
C. the buffer solution of gained is sprayed onto in Valeriana officinalis var. latifolia root powder, seal, thin film wrap up, in 40 ~ 50 DEG C ferment 5 ~ 7d；
D. the raw material after fermentation is put in the Vltrasonic device that frequency is 40KHz and extract under conditions of ultrasonic temperature 60 ~ 70 DEG C 2 ~ 3h, filters, and gained extracting solution is dried through concentrating under reduced pressure, organic solvent extraction, anhydrous sodium sulfate again, the step such as concentrating under reduced pressure, Obtain Valeriana officinalis var. latifolia root volatile oil.
The activity of the cellulase described in step b is 40 ~ 60U/mg.
Organic solvent described in step d is any one in petroleum ether or ether.
The pharmacologically active of Valeriana officinalis var. latifolia root volatile oil prepared by the present invention is verified by following external pharmacological evaluation:
(1) antiinflammatory experiment (rat polyclonal antibodies)
Take male mice 50, body weight 18 ~ 22g, often group 10, model group (edible soybean oil), positive controls (hydrogenation can Pine, 25mg/kg), administration component be low dose group (100mg/kg), middle dosage group (200mg/kg) and high dose group (400mg/ Kg), respectively after the reagent of lumbar injection 0.2mL variable concentrations, being coated with dimethylbenzene in mouse right ear, left ear maintains the original state, after 30min De-neck is put to death, and cuts two ear 8mm card punch and lays round auricle at same position respectively, and electronic balance precise weighing, with left and right Auricle weight is the poorest for swelling, calculates suppression ratio:
Suppression ratio=(blank group average swelling-equal swelling of administration group product)/average swelling × 100% of blank group
Table 1 Valeriana officinalis var. latifolia root volatile oil xylol cause mice auricle swelling impact (± S)
Group Number of animals Dosage (mg/kg) Swelling (mg) Ear swelling suppression ratio (%)
Model group 10 - 10.86±1.87
Positive controls 10 25 4.18±0.37▲▲ 61.51
Low dose group 10 100 7.65±1.34▲ 29.56
Middle dosage group 10 200 6.07±1.57▲▲ 44.11
High dose group 10 400 4.49±1.72▲▲ 58.66
Compare with blank group,▲P ＜ 0.05,▲▲P ＜ 0.01.
Being shown by table 1 result, positive controls, administration group compare with model group all significant, all can be the most right Anti-dimethylbenzene induced mice auricle edema, high dose group antiinflammatory action is fairly obvious, suitable with positive controls effect.
(2) analgesic experiment (acetic acid causes mouse writhing reaction)
A. experiment material
Animal: white mice in Kunming, body weight 22 ~ 26g, totally 50；
Equipment: syringe (1.0mL), balance
Medicine and reagent: 0.7% acetic acid (is now joined), 0.9% sodium chloride injection, hydrochloric acid dolantin 2mg/mL, Valeriana officinalis var. latifolia root is waved Hair oil be configured to Graded amounts be 5,10, the medicinal liquid of 20mg/mL.
B. method and step
Random is a component group by test white mice by 10, and one of which is blank group, by 0.2mL/10g body weight dose 0.9% sodium chloride injection is given with lumbar injection；Positive controls gives aspirin by 60mg/kg dosage with lumbar injection. Remaining for being given the trial drug group of Valeriana officinalis var. latifolia root volatile oil by various dose gradient gavage.The administration of each gastric infusion group is molten Agent is 0.2ml/10g body weight.Successive administration 5 days, is administered once daily.Within 5th day, being administered 20min pneumoretroperitoneum, to inject 0.7% acetic acid molten Liquid 0.2mL/ only, after observed and recorded injection acetum in 20min the writhing response of every mice (abdominal part back leg is upheld, buttocks Raise) number of times, and use SPSS15.0 software to carry out statistical analysis experimental result, evaluate medicine with mouse writhing response inhabitation rate Effect.
Suppression ratio %=(blank group writhing mean-medicine group writhing mean)/blank group writhing mean × 100%
C. experimental result
Experimental result is as shown in table 2:
The table 2 Dichlorodiphenyl Acetate writhing method threshold of pain experiment impact (n=10,± S)
Group Dosage (mg/kg) Writhing number of times Suppression ratio (%)
Blank group - 78.00±4.42
Aspirin group 60 41.53±3.08** 46.76
Low dose group 100 61.24±5.28* 21.49
Middle dosage group 200 52.24±6.76** 33.03
High dose group 400 42.71±10.02** 45.24
Note: compare with blank group,*P ＜ 0.05,**P ＜ 0.01.
Can cause the pain stimulation that mice is more lasting after injection acetic acid, mice occurs that abdominal part back leg is upheld repeatedly, and buttocks is high Play (writhing) reaction.Result shows, aspirin group, administration group compare with blank group, all have significant difference.Ah Si Woods Dichlorodiphenyl Acetate induced mice writhing number of times is decreased obviously (P ＜ 0.01), and clearly, its pain suppression ratio is analgesic activity 46.76%.Valeriana officinalis var. latifolia root volatile oil is respectively organized all can reduce in various degree and is reacted by the mouse writhing caused by acetic acid, has brighter Aobvious analgesic activity, and in dose dependent.
Preparation method of the present invention is simple, and gained volatile oil biological activity is high, and anti-inflammatory analgesic effect is obvious.
Further illustrate the present invention below in conjunction with detailed description of the invention, but the scope of protection of present invention is not limited to In following embodiments.
Detailed description of the invention:
Embodiment 1:
Valeriana officinalis var. latifolia root is pulverized, crosses 20 mesh sieves, take 1kg powder standby；The cellulase that the activity of 10g is 40u/mg is dissolved In the acetic acid/sodium acetate buffer of the pH4.0 of 500mL, mix homogeneously；The buffer solution of gained is sprayed onto Valeriana officinalis var. latifolia In root powder, sealing, thin film wraps up, in 40 DEG C of 5d that ferment；Raw material after fermentation is put in the Vltrasonic device that frequency is 40KHz Extracting 2h under conditions of ultrasonic temperature 60 DEG C, filter, gained extracting solution is again through concentrating under reduced pressure, petroleum ether extraction, anhydrous slufuric acid The steps such as sodium is dried, concentrating under reduced pressure, obtain Valeriana officinalis var. latifolia root volatile oil 17.2mL.
Embodiment 2:
Valeriana officinalis var. latifolia root is pulverized, crosses 20 mesh sieves, take 1kg powder standby；The cellulase that the activity of 20g is 50u/mg is dissolved In the acetic acid/sodium acetate buffer of the pH4.5 of 1L, mix homogeneously；The buffer solution of gained is sprayed onto Valeriana officinalis var. latifolia root In powder, sealing, thin film wraps up, in 50 DEG C of 7d that ferment；Will fermentation after raw material put in the Vltrasonic device that frequency is 40KHz in Extracting 2.5h under conditions of ultrasonic temperature 70 DEG C, filter, gained extracting solution is again through concentrating under reduced pressure, ether extraction, anhydrous sodium sulfate The steps such as dry, concentrating under reduced pressure, obtain Valeriana officinalis var. latifolia root volatile oil 20.8mL.
Embodiment 3:
Valeriana officinalis var. latifolia root is pulverized, crosses 20 mesh sieves, take 1kg powder standby；The cellulase that the activity of 30g is 60u/mg is dissolved In the acetic acid/sodium acetate buffer of the pH5.0 of 500mL, mix homogeneously；The buffer solution of gained is sprayed onto Valeriana officinalis var. latifolia In root powder, sealing, thin film wraps up, in 45 DEG C of 6d that ferment；Raw material after fermentation is put in the Vltrasonic device that frequency is 40KHz Extracting 3h under conditions of ultrasonic temperature 65 DEG C, filter, gained extracting solution is again through concentrating under reduced pressure, ether extraction, anhydrous sodium sulfate The steps such as dry, concentrating under reduced pressure, obtain Valeriana officinalis var. latifolia root volatile oil 15.9mL.

Claims (4)

1. the preparation method of a Valeriana officinalis var. latifolia root volatile oil, it is characterised in that comprise the steps:
A. Valeriana officinalis var. latifolia root is pulverized, cross 20 mesh sieves；
B. the cellulase of raw material weight 1 ~ 3% is dissolved in the acetic acid/sodium acetate of pH4.0 ~ 5.0 of raw material weight 0.5 ~ 1 times In buffer solution, mix homogeneously；
C. the buffer solution of gained is sprayed onto in Valeriana officinalis var. latifolia root powder, seal, thin film wrap up, in 40 ~ 50 DEG C ferment 5 ~ 7d；
D. the raw material after fermentation is put in the Vltrasonic device that frequency is 40KHz and extract under conditions of ultrasonic temperature 60 ~ 70 DEG C 2 ~ 3h, filters, and gained extracting solution is dried through concentrating under reduced pressure, organic solvent extraction, anhydrous sodium sulfate again, the step such as concentrating under reduced pressure, Obtain Valeriana officinalis var. latifolia root volatile oil.
The preparation method of a kind of Valeriana officinalis var. latifolia root volatile oil the most according to claim 1, it is characterised in that institute in step b The activity of the cellulase stated is 40 ~ 60U/mg.
The preparation method of a kind of Valeriana officinalis var. latifolia root volatile oil the most according to claim 1, it is characterised in that institute in step d The organic solvent stated is any one in petroleum ether or ether.
4. the Valeriana officinalis var. latifolia root volatile oil prepared according to the preparation method described in claim 1 ~ 3 has anti-inflammatory analgesic in preparation and makees Medicine in application.
CN201610302999.4A 2016-05-10 2016-05-10 A preparing method for valeriana officinalis var. latifolia root volatile oil and inflammation-diminishing and pain-easing applications of the volatile oil Pending CN106038623A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113896629A (en) * 2021-11-10 2022-01-07 遵义医科大学 Preparation method and application of gemma alkane type sesquiterpenoids

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113896629A (en) * 2021-11-10 2022-01-07 遵义医科大学 Preparation method and application of gemma alkane type sesquiterpenoids
CN113896629B (en) * 2021-11-10 2024-01-23 遵义医科大学 Preparation method and application of germacrane sesquiterpenoids

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