CN106038696A - A preparing method of hairyleaf litse fruit volatile oil and applications of the volatile oil - Google Patents
A preparing method of hairyleaf litse fruit volatile oil and applications of the volatile oil Download PDFInfo
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- CN106038696A CN106038696A CN201610303000.8A CN201610303000A CN106038696A CN 106038696 A CN106038696 A CN 106038696A CN 201610303000 A CN201610303000 A CN 201610303000A CN 106038696 A CN106038696 A CN 106038696A
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- volatile oil
- solani melongenae
- fructus solani
- clear fructus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/54—Lauraceae (Laurel family), e.g. cinnamon or sassafras
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/10—Preparation or pretreatment of starting material
- A61K2236/15—Preparation or pretreatment of starting material involving mechanical treatment, e.g. chopping up, cutting or grinding
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
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- General Health & Medical Sciences (AREA)
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Abstract
The invention relates to a preparing method of hairyleaf litse fruit volatile oil and applications of the volatile oil. The preparing method includes steps of (1) smashing a raw material, and sieving with a sieve having a size of 40 meshes, (2) performing continuous counter-flow ultrasonic extraction, and (3) extracting with an organic solvent to obtain the hairyleaf litse fruit volatile oil. Pharmacodynamic evaluation proves that the volatile has obvious antiallergic and antasthmatic activity, and can be used for preparing medicines for anaphylactic diseases including allergic asthma and allergic rhinitis.
Description
Technical field
The invention belongs to pharmaceutical technology field, be specifically related to the preparation method and applications of a kind of clear Fructus Solani melongenae volatile oil.
Background technology
Clear Fructus Solani melongenae has another name called Fructus Litseae pungentis, Fructus Litseae, Fructus Linderae Glaucae, for the dry mature fruit of canella Litsea mollifolia, has
Warming spleen and stomach for dispelling cold, effect of regulating QI to relieve pain.Main component monoterpene and sesquiterpenoids in clear Fructus Solani melongenae volatile oil, including pinene, camphene, lemon
The compositions such as lemon aldehyde, citronellal, linalool, Camphora and geranial, wherein citral is main component.
At present, the research to Fructus Litseae is concentrated mainly on its chemical composition analysis, and to its pharmacology activity research relatively
Few.
Allergic rhinitis is that the commonly encountered diseases of current nose section, frequently-occurring disease, and touching difficulty heal.Doctor trained in Western medicine lacks specific treatment hands at present
Section, and Chinese medicine has defects such as proving effective slowly, consumption is big.
Summary of the invention
It is an object of the invention to provide the preparation method and application of a kind of clear Fructus Solani melongenae volatile oil, this volatile oil antiallergic,
Asthma effect is obvious.
The present invention completes by following technical solution, and particular content includes: the preparation method of clear Fructus Solani melongenae and in preparation
Application in antianaphylactic medicine.
A. clear Fructus Solani melongenae is pulverized, cross 40 mesh sieves；
B. by clear Fructus Solani melongenae powder add raw materials quality 3 ~ 5 times 70 ~ 80% ethanol solution, in temperature 60 ~ 70 DEG C, frequency
Solid-liquid separation after Continuous Countercurrent Extraction 2 ~ 3h under conditions of 40KHz, obtains concentrated solution after concentrating under reduced pressure；
C. being extracted 2 ~ 3 times through organic solvent by gained concentrated solution, combining extraction liquid, anhydrous sodium sulfate is dried, concentrating under reduced pressure, i.e.
Obtain clear Fructus Solani melongenae volatile oil.
Organic solvent described in step c is any one in petroleum ether or ether.
The pharmacodynamic study of clear Fructus Solani melongenae volatile oil prepared by the present invention:
(1) histamine-acetylcholine is sprayed to draw and is breathed heavily test
Take white mice 60(body weight 192 ~ 200g) only, male and female half and half, white mice is put into one by one bell jar, sprays into 2% chlorine equably
Change acetylcholine and 1% histamine phosphate mixed liquor 15s, observe and cause that animal breath is rapid, twitch and tumble time to fall
The tipping rolling time animal less than 120s is the qualified animal of asthma.Animal qualified for asthma is randomly divided into 5 groups, often group 10,
It is respectively blank group (A), low dose group (B), middle dosage group (C), high dose group (D) and positive controls (shake bile-fritillary
Dissipate, E), B, C, D group gavages clear Fructus Solani melongenae volatile oil, and A group gavage is to isometric(al) 0.5%CMC Na5mL/kg, and each group dosage is shown in Table
1, once a day, continuous 7 days, last be administered after 1h, draw breathe heavily according to said method spraying, draw when breathing heavily vapor recording mice tumble
Between, the results are shown in Table 1.
Table 1 clear Fructus Solani melongenae volatile oil medicine is caused white mice asthma impact (n=10,± S)
| Group | Dosage | Before administration (s) | After administration (s) | Rate elongation (%) |
| A | 5mL/kg | 87.1±12.8 | 89.3±13.5 | 2.5 |
| B | 0.2g/kg | 88.4±12.4 | 143.2±21.1* | 61.99 |
| C | 0.4g/kg | 89.1±13.5 | 158.9±49.2* | 78.34 |
| D | 0.6g/kg | 88.9±14.6 | 169.6±50.1* | 90.78 |
| E | 0.4 g/kg | 89.3±12.8 | 159.3±39.6* | 78.39 |
Note: compare with blank group*P ＜ 0.01
Result shows, white mice is all had certain effect by the basic, normal, high dosage group of clear Fructus Solani melongenae volatile oil and positive controls,
The tumble time of mice can be obviously prolonged, and, its effect increases with concentration and strengthens, and the rate elongation of high dose group is relatively
Shake bile-fritillary dissipates and rises appreciably, and its effect is notable.
(2) passive cutaneous anaphylaxis test (PCA)
Taking wistra rat 50, male and female half and half, body weight 190 ~ 200g, be randomly divided into 5 groups, often group 10, it is blank right to be respectively
According to group (A), low dose group (B), middle dosage group (C), high dose group (D) and positive controls (shake bile-fritillary dissipates, E), A group is given
0.5%CMC10mL/kg, B, C, D group give 0.2g/kg, 0.4g/kg, 0.6g/kg respectively, and E group dissipates 0.4 g/kg to shake bile-fritillary,
Once a day, continuous gastric infusion 5 days.Being administered ridge depilation in latter 2 days, spinal column both sides, according to center line 1.5cm, respectively take 2 points, often
Point interval 2cm, in depilation portion intradermal injection difference dilution factor (1:16:32) rat ovaserum, 0.1mL/ point, is total to next day
4 points, and before last is administered 2h intravenous 1% Evans blue and ovalbumin 10mg/kg mixed liquor 1mL/, sacrificed by decapitation after 2h.
Observation index: open skin of back, measures the locus coeruleus diameter that Evans blue oozes out.Calculate PCA inhibition percentage (%).PCA
Inhibition percentage=(blank group locus coeruleus diameter-medication group locus coeruleus diameter)/blank group locus coeruleus diameter × 100%.Result
It is shown in Table 2:
Impact that models of passive skin irritability of rats is reacted by table 2 clear Fructus Solani melongenae volatile oil (n=10,± S)
Note: compared with blank group, *P ＜ 0.01
Result shows, compared with blank group, clear Fructus Solani melongenae volatile oil basic, normal, high dosage group antiserum dilution factor 1:16,
All can show significance suppression locus coeruleus diameter during 1:32, show that passive anaphylaxis is had certain suppression to make by this volatile oil
With, its effect slightly below shake bile-fritillary dissipates.
Further illustrate the present invention below in conjunction with detailed description of the invention, but the scope of protection of present invention is not limited to
In following embodiments.
Detailed description of the invention:
Embodiment 1:
Clear Fructus Solani melongenae is pulverized, crosses 40 mesh sieves；By the ethanol solution of the 70% of clear Fructus Solani melongenae powder addition raw materials quality 3 times, in temperature 60
DEG C, solid-liquid separation after Continuous Countercurrent Extraction 2h under conditions of frequency 40KHz, after concentrating under reduced pressure concentrated solution；Gained is concentrated
Liquid is through petroleum ether extraction 2 times, combining extraction liquid, and anhydrous sodium sulfate is dried, concentrating under reduced pressure, obtains the clear Fructus Solani melongenae volatile oil of yellow,
Yield is 2.3%.
Embodiment 2:
Clear Fructus Solani melongenae is pulverized, crosses 40 mesh sieves；By the ethanol solution of the 80% of clear Fructus Solani melongenae powder addition raw materials quality 5 times, in temperature 65
DEG C, solid-liquid separation after Continuous Countercurrent Extraction 2h under conditions of frequency 40KHz, after concentrating under reduced pressure concentrated solution；Gained is concentrated
Liquid extracts 2 times through ether, combining extraction liquid, and anhydrous sodium sulfate is dried, concentrating under reduced pressure, obtains the clear Fructus Solani melongenae volatile oil of yellow,
Rate is 3.1%.
Embodiment 3:
Clear Fructus Solani melongenae is pulverized, crosses 40 mesh sieves；By the ethanol solution of the 75% of clear Fructus Solani melongenae powder addition raw materials quality 4 times, in temperature 70
DEG C, solid-liquid separation after Continuous Countercurrent Extraction 3h under conditions of frequency 40KHz, after concentrating under reduced pressure concentrated solution；Gained is concentrated
Liquid is through petroleum ether extraction 2 times, combining extraction liquid, and anhydrous sodium sulfate is dried, concentrating under reduced pressure, obtains the clear Fructus Solani melongenae volatile oil of yellow,
Yield is 2.8%.
Claims (3)
1. the preparation method of a clear Fructus Solani melongenae volatile oil, it is characterised in that comprise the steps:
A. clear Fructus Solani melongenae is pulverized, cross 40 mesh sieves；
B. by clear Fructus Solani melongenae powder add raw materials quality 3 ~ 5 times 70 ~ 80% ethanol solution, in temperature 60 ~ 70 DEG C, frequency
Solid-liquid separation after Continuous Countercurrent Extraction 2 ~ 3h under conditions of 40KHz, obtains concentrated solution after concentrating under reduced pressure；
C. being extracted 2 ~ 3 times through organic solvent by gained concentrated solution, combining extraction liquid, anhydrous sodium sulfate is dried, concentrating under reduced pressure, i.e.
Obtain clear Fructus Solani melongenae volatile oil.
The preparation method of a kind of clear Fructus Solani melongenae volatile oil the most according to claim 1, it is characterised in that described in step c
Organic solvent is any one in petroleum ether or ether.
3. clear Fructus Solani melongenae volatile oil the answering in the medicine preparing anti-allergic effects prepared according to method described in claim 1 ~ 2
With.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114748530A (en) * | 2022-06-06 | 2022-07-15 | 杭州市西溪医院 | Application of traditional Chinese medicine cubeb extract in preparing chronic urticaria medicine |
-
2016
- 2016-05-10 CN CN201610303000.8A patent/CN106038696A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114748530A (en) * | 2022-06-06 | 2022-07-15 | 杭州市西溪医院 | Application of traditional Chinese medicine cubeb extract in preparing chronic urticaria medicine |
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Application publication date: 20161026 |