CN106038680A - Pharmaceutical composition for treating gastric cancer and preparation method thereof - Google Patents
Pharmaceutical composition for treating gastric cancer and preparation method thereof Download PDFInfo
- Publication number
- CN106038680A CN106038680A CN201610462763.7A CN201610462763A CN106038680A CN 106038680 A CN106038680 A CN 106038680A CN 201610462763 A CN201610462763 A CN 201610462763A CN 106038680 A CN106038680 A CN 106038680A
- Authority
- CN
- China
- Prior art keywords
- pharmaceutical composition
- preparation
- gastric cancer
- extract
- macroporous adsorbent
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/48—Fabaceae or Leguminosae (Pea or Legume family); Caesalpiniaceae; Mimosaceae; Papilionaceae
- A61K36/481—Astragalus (milkvetch)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/39—Complex extraction schemes, e.g. fractionation or repeated extraction steps
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/55—Liquid-liquid separation; Phase separation
Landscapes
- Health & Medical Sciences (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Alternative & Traditional Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicinal Preparation (AREA)
Abstract
The invention discloses a pharmaceutical composition for treating gastric cancer and a preparation method thereof. The pharmaceutical composition is characterized in that the pharmaceutical composition is a composition prepared by taking total saponins of radix actinidiae chinensis, total flavonoids and total saponins of radix astragali seu hedysari as effective components. The preparation method of the pharmaceutical composition is characterized by respectively extracting the two medicine materials by using alcohol, thus obtaining extracts; after drying and smashing the extracts, mixing the dried and smashed extracts in proportion, thus obtaining powder, wherein the obtained powder can be prepared into all kinds of oral solid preparations which are clinically accepted through a traditional Chinese medicine preparation conventional preparation method, and the oral solid preparations comprise capsules, granules, tablets, pills and the like. A traditional Chinese medicine preparation for treating the gastric cancer has no side effects, and is capable of effectively treating the gastric cancer and improving the living quality of a patient suffering from the gastric cancer.
Description
Technical field
The present invention relates to a kind of pharmaceutical composition, particularly relate to a kind of pharmaceutical composition treating gastric cancer and preparation side thereof
Method.
Background technology
Gastric cancer is the modal malignant tumor of digestive system, and it is the first that its mortality rate occupies malignant tumor.Incidence gastric cancer has substantially
Region difference, higher than southern area is evident as with coastal region in east China incidence gastric cancer rate in the northwest of China, this is with above-mentioned
The dietary habit in area is closely related.On the other hand.China's high incidence area of gastric cancer adult's Helicobacter Pylori Infection Rate is high.Secondly,
The high incidence of gastric cancer is also relevant to inherited genetic factors.At present, the treatment for gastric cancer take more operative treatment, chemotherapy and
The other treatment methods such as radiotherapy, thermotherapy, immunization therapy, traditional Chinese medical herbal treatment, various methods are being saved patient vitals, are being improved patient
Quality of life aspect all serves certain effect, but there is also some problems, and especially Postoperative Recurrence of Advanced Gastric Cancer rate is high,
Survival rate is low, and many chemotherapeutics clinical therapeutic efficacies are unsatisfactory, and toxicity is big, makes many patients be difficult to bear and put
Abandon treatment, have a strong impact on quality of life and the life cycle of patient.Chinese medicine cancer, is the Therapeutic Method of China's uniqueness, with
The operation of doctor trained in Western medicine, radiotherapy, chemotherapy compare, and are respectively arranged with its chief.Use properly, can learn from other's strong points to offset one's weaknesses, improve curative effect.
Radix Actinidiae Chinensis is Actinidiaceae Actinidia arguta Sieb.et Zucc Actinidia arguta (Sieb.et Zucc.) Planch
Root, once recorded in version " Chinese Pharmacopoeia " in 1987.China is main producing region, be distributed mainly on the Northeast of China and Hebei, Shanxi,
Also there are distribution in ground, Korea and the Japan such as Shandong, Anhui, Zhejiang, Jiangxi, Henan, Hubei, Yunnan.Radix Actinidiae Chinensis sour in the mouth, puckery, cool in nature,
There is the effects such as heat-clearing and toxic substances removing, clearing away heat-damp and promoting diuresis, expelling wind and removing dampness, diuresis hemostasis, removing toxic substances and promoting subsidence of swelling, hemostasis.Modern pharmacological research shows
Radix Actinidiae Chinensis Chinensis extrat has the multiple pharmacology such as enhancing immunologic function, antioxidation, blood fat reducing, mutation, anti-distortion and antitumor and makees
With, be widely used in cancer in digestive system, jaundice, dyspepsia, vomit, the treatment of the disease such as diarrhoea.
The Radix Astragali is the conventional Chinese medicine of strengthening the body resistance, has effect of invigorating QI to consolidate the body surface resistance, diuresis, toxin expelling, granulation promoting.Master in the Radix Astragali
Wanting chemical composition to include saponins, flavonoid, polysaccharide etc., wherein Radix Astragali saponin is its main active.The most extensive
For treat circulation, nerve, digest, breathe, the disease such as endocrine and blood system.
At present, with Radix Actinidiae Chinensis for principal agent Compatibility with Radix Astragali, the effective elements of the medicine in Hydrolysis kinetics two taste, with the total soap of Radix Actinidiae Chinensis
Glycosides, total flavones and Radix Astragali total saponins are that the pharmaceutical preparation of medicine composition treatment gastric cancer has no report.Such as prior art Granted publication
Number: the Chinese invention patent of CN103585303B, this invention relates to a kind of pharmaceutical composition treating gastric cancer and preparation method thereof,
It is characterized in that: this pharmaceutical composition is to be made up of following crude drug by weight: Dorema ammoniacum resin 3-5 part, meat torr fruit 1-4
2-5 part, vulture excrement 1-3 part, spiral shell operculum 0.8-1.5 part, the lazurite 1-3 parts such as part, mesh Herba Lepisori Thunbergiani 3-8 part, Song Sheng.The medicine of this invention
Combine and treatment gastric cancer is had good using value.Chinese invention such as prior art Authorization Notice No.: CN101084982B
Patent, this invention discloses the preparation method of a kind of Radix Astragali total saponins, comprises the steps: that (1) takes Milkvetch Root in order,
Add solvent, extract again after solution is adjusted to alkalescence, obtain extracting solution；(2) macroporous type alkali anion exchange on extracting solution
Post, first by aqueous alkali and pure water rinsing, flushing liquor discards, then uses aquiferous ethanol eluting, and eluent concentrates, and obtains Radix Astragali total saponins.
The extraction separation efficiency of the Radix Astragali total saponins preparation method of this invention is high, and Radix Astragali total saponins content is high, can keep higher physiology
Activity.Such as the Chinese invention patent of prior art Authorization Notice No.: CN103405586B, this disclosure of the invention a kind of Herba Spirodelae grass is total
Flavone extract, it is prepared from as steps described below: 1) takes Herba Spirodelae grass herb powder, adds the second of 8 ~ 15 times 20 ~ 90%
Alcohol, heating and refluxing extraction 1 ~ 3 time, each 0.5 ~ 2 hour, united extraction liquid；2) extracting solution is evaporated to without alcohol taste, passes through
Macroporous adsorbent resin, is first eluted to effluent with ethanol solution less than 20% of water or concentration transparent, then with concentration be 40 ~
4 ~ 8 times of column volumes of the ethanol solution eluting of 90%, collect the ethanol elution of 40 ~ 90%, concentrate, and are dried, and described Herba Spirodelae grass is total yellow
Containing mass percent in ketone extract is the total flavones of 50 ~ 99%.This invention extract has antiallergic action, detumescence, antipruritic
Effect.
Summary of the invention
It is an object of the present invention to provide one to have no side effect, curative effect significantly treats pharmaceutical composition and the preparation thereof of gastric cancer
Method.
The present invention is practiced by following technical solution:
The present invention is raw materials used:
Radix Actinidiae Chinensis Actinidia arguta (Sieb.et Zucc.) Planch, the dry root of Actinidiaceae Actinidia arguta Sieb.et Zucc
Radix Actinidiae Chinensis, Radix Astragali Astragalus membranaceus (Fisch.) Bge. var. mongholicus (Bge.) Hsiao
Or Astragalus membranaceus (Fisch.) Bge, leguminous plant Radix Astagali or the dry root of Radix Astragali.
A kind of pharmaceutical composition treating gastric cancer, the active component of its pharmaceutical composition include for: Radix Actinidiae Chinensis total saponins is total
Flavone and Radix Astragali total saponins.
The weight ratio of Radix Actinidiae Chinensis total saponins extractive of general flavone and Radix Astragali total saponins extract is 1-9:9-1.
As preferably, the weight ratio of Radix Actinidiae Chinensis total saponins total flavones and Radix Astragali total saponins extract is 8:2.
Described pharmaceutical composition, with Chinese medicine or chemical drugs or natural drug composition capsule or tablet or granule or drip
The medicine of pill treatment gastric cancer.
A kind of preparation method of the pharmaceutical composition treating gastric cancer, it comprises the following steps:
1) take Radix Actinidiae Chinensis medical material to add alcohol heating reflux that mass fraction is 35%-97% and extract 1-4 time, by weight, each second
The consumption of alcoholic solution is 6-17 times of raw material dosage, and extraction time is 2-6 hour, merges ethanol extract；
2) using enriching and purifying macroporous resin, using mass fraction is 10%-97% ethanol elution, and elution speed is 4-
10BV/h, eluent is evaporated to relative density at the thick paste that 25-50 DEG C is 1.20-1.25, then dry, pulverize, obtains rattan
Radix pyri total saponins extractive of general flavone powder, standby；
3) take Milkvetch Root to add alcohol heating reflux that mass fraction is 35%-97% and extract 1-3 time, by weight, each ethanol
The consumption of solution is 8-12 times of raw material dosage, and extraction time is 2-6 hour, merges ethanol extract；
4) using enriching and purifying macroporous resin, using mass fraction is 10%-97% ethanol elution, and elution speed is 4-
10BV/h, eluent is evaporated to relative density at the thick paste that 25-50 DEG C is 1.20-1.25, then dry, pulverize, obtains Huang
Stilbene total saponin extracts powder, standby；
5) mix above-mentioned steps 2 according to aforementioned pharmaceutical compositions weight ratio), 4) extract powder that respectively obtains.
As preferably, described step 2), 4) in the vacuum drying process being dried as resolving material, and drying condition is
35-85 DEG C, under-0.56mpa to 0.10mpa.
Described macroporous adsorbent resin is DM-301 type macroporous adsorbent resin or HPD-100 type macroporous adsorbent resin or X-5
Type macroporous adsorbent resin or AB-8 type macroporous adsorbent resin or NKA-9 type macroporous adsorbent resin or D-101 type macroporous adsorbent resin.
As preferably.Described macroporous adsorbent resin is HPD-100 type macroporous adsorbent resin, can become with highly enriched drug effect
Point, go the removal of impurity, reduce taking dose, improve the utilization rate of crude drug.
Specific embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
Embodiment 1:
The preparation of capsule
1) take Radix Actinidiae Chinensis medical material to add alcohol heating reflux that mass fraction is 75% and extract 2 times, by weight, each ethanol solution
Consumption is raw material dosage 12 times, extraction time is 3 hours, merge ethanol extract.Use HPD-100 type macroporous absorption tree
Fat enriching and purifying, is 45% ethanol elution with the mass fraction of raw material dosage 12 times amount, and elution speed is 6BV/h.Eluent reduces pressure
It is concentrated into relative density at the thick paste that 45 DEG C is 1.20-1.25, at 35-45 DEG C, is vacuum dried under-0.09mpa-0.10mpa, powder
Broken, obtain Radix Actinidiae Chinensis total saponins extractive of general flavone powder, standby；
2) take Milkvetch Root to add alcohol heating reflux that mass fraction is 60% and extract 3 times, by weight, each ethanol solution
Consumption is 10 times of raw material dosage, and extraction time is 2 hours, merges ethanol extract.Use HPD-100 type macroporous adsorbent resin
Enriching and purifying, is 30% ethanol elution with the mass fraction of raw material dosage 10 times amount, and elution speed is 8BV/h.Eluent decompression is dense
It is reduced to relative density at the thick paste that 50 DEG C is 1.20-1.25, at 35-45 DEG C, is vacuum dried under-0.80mpa-0.10mpa, powder
Broken, obtain Radix Astragali total saponins extract powder, standby；
3) in Radix Actinidiae Chinensis total saponins extractive of general flavone powder, the ratio that weight ratio is 8:2 of Radix Astragali total saponins extract powder
Mix two kinds of fine powders, kept dry.
4) adding starch, magnesium stearate mixing in fine powder, use conventional processing equipment to make capsule, capsule every fills
0.30g。
Embodiment 2
The preparation of granule
1) take Radix Actinidiae Chinensis medical material to add alcohol heating reflux that mass fraction is 60% and extract 3 times, by weight, each ethanol solution
Consumption is raw material dosage 10 times, extraction time is 3 hours, merge ethanol extract.Use HPD-100 type macroporous absorption tree
Fat enriching and purifying, is 50% ethanol elution with the mass fraction of raw material dosage 8 times amount, and elution speed is 8BV/h.Eluent reduces pressure
It is concentrated into relative density at the thick paste that 50 DEG C is 1.20-1.25, at 45-50 DEG C, is vacuum dried under-0.09mpa-0.10mpa, powder
Broken, obtain Radix Actinidiae Chinensis total saponins extractive of general flavone powder, standby；
2) take Milkvetch Root to add alcohol heating reflux that mass fraction is 75% and extract 2 times, by weight, each ethanol solution
Consumption is 10 times of raw material dosage, and extraction time is 3 hours, merges ethanol extract.Use HPD-100 type macroporous adsorbent resin
Enriching and purifying, is 50% ethanol elution with the mass fraction of raw material dosage 12 times amount, and elution speed is 10BV/h.Eluent reduces pressure
It is concentrated into relative density at the thick paste that 50 DEG C is 1.20-1.25, at 45-48 DEG C, is vacuum dried under-0.80mpa-0.10mpa, powder
Broken, obtain Radix Astragali total saponins extract powder, standby；
3) mix in the ratio that weight ratio is 8:2 of Radix Actinidiae Chinensis total saponins extractive of general flavone powder Radix Astragali total saponins extract powder
Close two kinds of fine powders, kept dry.
4) fine powder adds Icing Sugar, dextrin mixing, with the ethanol solution granulation that mass fraction is 70%, dry, granulate, dress
Bag, every bag of weight 10g.
Embodiment 3
The preparation of tablet
1) take Radix Actinidiae Chinensis medical material to add alcohol heating reflux that mass fraction is 50% and extract 3 times, by weight, each ethanol solution
Consumption is raw material dosage 8 times, extraction time is 2 hours, merge ethanol extract.Use HPD-100 type macroporous absorption tree
Fat enriching and purifying, is 60% ethanol elution with the mass fraction of raw material dosage 12 times amount, and elution speed is 10BV/h.Eluent subtracts
Pressure is concentrated into relative density at the thick paste that 50 DEG C be 1.20-1.25, at 45-48 DEG C, and vacuum drying under-0.08mpa-0.10mpa,
Pulverize, obtain Radix Actinidiae Chinensis total saponins extractive of general flavone powder, standby；
2) take Milkvetch Root to add alcohol heating reflux that mass fraction is 50% and extract 3 times, by weight, each ethanol solution
Consumption is 12 times of raw material dosage, and extraction time is 2 hours, merges ethanol extract.Use HPD-100 type macroporous adsorbent resin
Enriching and purifying, is 70% ethanol elution with the mass fraction of raw material dosage 8 times amount, and elution speed is 6BV/h.Eluent decompression is dense
It is reduced to relative density at the thick paste that 50 DEG C is 1.20-1.25, at 45-50 DEG C, is vacuum dried under-0.90mpa-0.10mpa, powder
Broken, obtain Radix Astragali total saponins extract powder, standby；
3) mix in the ratio that weight ratio is 8:2 of Radix Actinidiae Chinensis total saponins extractive of general flavone powder Radix Astragali total saponins extract powder
Close two kinds of fine powders, kept dry.
4) fine powder adds Icing Sugar, dextrin mixing, with the ethanol solution granulation that mass fraction is 70%, dry, granulate, adds
Enter magnesium stearate mixing, use conventional processing equipment to make tablet, every tablet weight 0.35g.
Experimental example 1:
Cell experiment
1, experimental cell strain: BGC-823 cell is provided by Shanghai Inst. of Life Science, CAS cellular resources center.
2, Experimental agents: pharmaceutical composition powder of the present invention (the Radix Actinidiae Chinensis total saponins extracted according to embodiment 1 extracting method
Extractive of general flavone and Radix Astragali total saponins extract, by Radix Actinidiae Chinensis total saponins extractive of general flavone, Radix Astragali total saponins extract
The part by weight that weight ratio is 8:2 mixing two kinds of fine powders.)
3, experimental technique: take BGC-823 cell, by the corresponding culture medium containing 10% hyclone at 37 DEG C, 5%C02 condition
Lower cultivation, after passing for 3 generations, trophophase cell of taking the logarithm is for testing.Cell is made into single cell suspension, inoculates with 1 × 106/mL
In 96 porocyte culture plates, every hole adds tumor cell suspension 100 μ L, then is separately added into 6 kinds of variable concentrations rattans of each 100 μ L
Radix pyri extracting solution (pharmaceutical composition powder diluent of the present invention, concentration is respectively 200,100,50,25,12.5,6.25ug/
Ml), if blank control wells adds 1640 culture fluid, experiment contrast hole adds tumor cell, and often group is 3 multiple holes.96 orifice plates are put
5%CO2 incubator (37 DEG C) cultivates 48h, adds 5mg/mlMTT20 μ L, after continuing to cultivate 4h, abandons supernatant, adds DMSO150 μ L,
10min is dissolved in vibration, and microplate reader detects every hole absorption value at 490nm, calculates pharmaceutical composition by suppression ratio computing formula and presses down
The percentage rate of stomach cancer cell processed, the results are shown in Table 1, determines optimum medicine concentration.
Tumour inhibiting rate computing formula tumour inhibiting rate=1-(OD experimental group-OD blank group)/(OD experiment contrast group-OD blank group)
×100%
The pharmaceutical composition of the present invention of the table 1 variable concentrations suppression ratio to BGG-233 cell
Table 1 result shows, the Radix Actinidiae Chinensis total saponins extractive of general flavone extracted according to embodiment 1 extracting method and the total soap of the Radix Astragali
Glucoside extract, by weight the part by weight mixing fine powders for 8:2, has tumor cell in 50-200ug/ml concentration range
Having preferable suppression ratio, especially 50ug/ml control of the concentration rate is maximum.
Experimental example 2:
Pharmacodynamic experiment
1, animal packet and modeling method: (experiment of Beijing dimension tonneau China is dynamic for male and female half and half, body weight 200 ~ 220g for 90 KM rats
Thing Technology Co., Ltd. provides, and licence is numbered: SCXK (capital) 2012-0001), be divided into normal group, model by table of random numbers method
Group, low dose group, middle dosage group, high dose group, positive drug group, often group 15.Normal group, not modeling, supply normal diet
And freely drink water, totally 30 weeks.Model group gavages tap water 4mL/kg 1 time every day, after 6h, gives 120 μ g/ml methyl nitro nitrous
Base guanidine and 10%NaCl mixed liquor, give 40% ethanol gavage simultaneously, and 2mL/ only, 2 times a week, totally 20 weeks, continues to raise 10 weeks, processes
The same normal group of method.Positive drug group, from modeling the 1st day, every day gavaged all-trans-retinoic acid, every 4mL/kg, 6h for 1 time
After, remaining processing method is identical with model group.Pressing body surface area to convert with 70kg adult's consumption, compositions group is basic, normal, high
Dosage is respectively 0.20g/kg, 0.40g/kg, 0.80g/kg；Given low 1 mL/100g body weight, remaining processing method and Wei Jia
Acid treatment group is identical.All rats are all put to death at the 30th weekend, and routine is cutd open and tested.After each organ disease of perusal, take stomach, liver,
The internal organs such as spleen, lung, kidney are fixed in 10% neutral formalin liquid.After 24h, conventional dehydration, paraffin embedding, preparation section, observe
With or without cancerometastasis and other pathological changes.According to literature method and formula, calculate canceration suppression ratio.Experimental result is shown in Table 2.
Canceration suppression ratio=(matched group carcinogenesis rate one experimental group carcinogenesis rate)/matched group carcinogenesis rate × 100%
The impact on causing gastric carcinoma mouse canceration suppression ratio of table 2 pharmaceutical composition of the present invention
* note: P < 0.05 compared with matched group
In experimentation, animal general status is good.Animal is active, and fur gloss, diet is without exception.90 rats were being tested
Because filling reasons such as feeding improper (wearing out esophagus etc.), pressurized dead 15 altogether in journey, dead because of Other diseases and cancerometastasis without 1 example
Person.30 13 mices that weekend, matched group was put to death, it is 100% that precancerous lesion, Precancerous Lesions rate all occur, and shows this mould
Type is reliable and stable.The pharmaceutical composition giving the present invention can significantly inhibit canceration rate.
Experimental example 3:
The animal acute toxicity research of Chinese medicinal composition capsules of the present invention
Selecting healthy Kun Ming mice, be administered with gavage method, observe the acute toxic reaction of the capsule of preparation, result is given
The maximum tolerated dose of medicine > 12.5g/kg, be equivalent to 250 times of clinical application amount.Matched group is to the distilled water of Isodose, continuously
Take medicine 7d.The general health of result white mice is good, and hair color is smooth, and appetite is normal, defecates without exception, during whole administration
Animal dead phenomenon do not occur, after experiment terminates, administration group white mice average weight rate of increase is identical with matched group.
The present invention have curative effect substantially, the compositions advantage simplifying, have no side effect.
Above-described embodiment is only case, details to technical solution of the present invention under without departing from the spirit and scope of the present invention
Modify with form or replace, each falling within protection scope of the present invention.
Claims (8)
1. the pharmaceutical composition treating gastric cancer, it is characterised in that: the active component of pharmaceutical composition is Radix Actinidiae Chinensis total saponins
Total flavones and Radix Astragali total saponins.
Pharmaceutical composition the most according to claim 1, it is characterised in that: described Radix Actinidiae Chinensis total saponins extractive of general flavone
It is 1-9:9-1 with the weight ratio of Radix Astragali total saponins extract.
Pharmaceutical composition the most according to claim 1, it is characterised in that: described Radix Actinidiae Chinensis total saponins total flavones and the Radix Astragali
The weight ratio of total saponin extracts is 8:2.
4. according to the pharmaceutical composition described in claim 1 or 2 or 3, described pharmaceutical composition can with Chinese medicine or chemical drugs or
Natural drug forms capsule or tablet or granule or the medicine of drop pill treatment gastric cancer.
5. the preparation method of the pharmaceutical composition treating gastric cancer, it is characterised in that following steps:
Take Radix Actinidiae Chinensis medical material to add alcohol heating reflux that mass fraction is 35%-97% and extract 1-4 time, by weight, each ethanol
The consumption of solution is 6-17 times of raw material dosage, and extraction time is 2-6 hour, merges ethanol extract；
Using enriching and purifying macroporous resin, using mass fraction is 10%-97% ethanol elution, and elution speed is 4-10BV/
H, eluent is evaporated to relative density at the thick paste that 25-50 DEG C is 1.20-1.25, then dry, pulverize, obtains Radix Actinidiae Chinensis total
Saponin extractive of general flavone powder, standby；
Taking Milkvetch Root to add alcohol heating reflux that mass fraction is 35%-97% and extract 1-3 time, by weight, each ethanol is molten
The consumption of liquid is 8-12 times of raw material dosage, and extraction time is 2-6 hour, merges ethanol extract；
Using enriching and purifying macroporous resin, using mass fraction is 10%-97% ethanol elution, and elution speed is 4-10BV/
H, eluent is evaporated to relative density at the thick paste that 25-50 DEG C is 1.20-1.25, then dry, pulverize, obtains the total soap of the Radix Astragali
Glucoside extract powder, standby；
Mix above-mentioned steps 2 according to the pharmaceutical composition weight ratio described in Claims 2 or 3), 4) the extract powder that respectively obtains
End.
The preparation method of pharmaceutical composition the most according to claim 4, it is characterised in that: described is dried as resolving material
Vacuum drying process, and drying condition is 35-85 DEG C, under-0.56mpa to 0.10mpa.
7. according to the preparation method of the pharmaceutical composition described in claim 4, it is characterised in that: described macroporous adsorbent resin is
DM-301 type macroporous adsorbent resin or HPD-100 type macroporous adsorbent resin or X-5 type macroporous adsorbent resin or AB-8 type macropore are inhaled
Attached resin or NKA-9 type macroporous adsorbent resin or D-101 type macroporous adsorbent resin.
The preparation method of pharmaceutical composition the most according to claim 4, it is characterised in that: described macroporous adsorbent resin is
HPD-100 type macroporous adsorbent resin.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610462763.7A CN106038680A (en) | 2016-06-24 | 2016-06-24 | Pharmaceutical composition for treating gastric cancer and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610462763.7A CN106038680A (en) | 2016-06-24 | 2016-06-24 | Pharmaceutical composition for treating gastric cancer and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106038680A true CN106038680A (en) | 2016-10-26 |
Family
ID=57169064
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610462763.7A Pending CN106038680A (en) | 2016-06-24 | 2016-06-24 | Pharmaceutical composition for treating gastric cancer and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN106038680A (en) |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1093594A (en) * | 1993-12-13 | 1994-10-19 | 邓庆安 | A kind of medicine and compound method thereof for the treatment of tumor |
| CN1098934A (en) * | 1994-05-09 | 1995-02-22 | 邓庆安 | A kind of anti-cancer composition and preparation method thereof |
| CN102028924A (en) * | 2009-09-25 | 2011-04-27 | 张士舜 | Medicinal composition for late gastric cancer |
-
2016
- 2016-06-24 CN CN201610462763.7A patent/CN106038680A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1093594A (en) * | 1993-12-13 | 1994-10-19 | 邓庆安 | A kind of medicine and compound method thereof for the treatment of tumor |
| CN1098934A (en) * | 1994-05-09 | 1995-02-22 | 邓庆安 | A kind of anti-cancer composition and preparation method thereof |
| CN102028924A (en) * | 2009-09-25 | 2011-04-27 | 张士舜 | Medicinal composition for late gastric cancer |
Non-Patent Citations (2)
| Title |
|---|
| 史作清等: "《吸附分离树脂在医药工业中的应用》", 29 February 2008, 化学工业出版社 * |
| 滕坤等: "大孔吸附树脂纯化藤梨根中总黄酮工艺优选", 《医药导报》 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Li et al. | A systematic review on botany, processing, application, phytochemistry and pharmacological action of Radix Rehmnniae | |
| CN102258563B (en) | Anti-cancer medicinal composition and Chinese medicinal composition | |
| CN102670994A (en) | Composition capable of relieving physical fatigue and preparation method of composition | |
| CN102114099B (en) | Traditional Chinese medicine preparation for inhibiting liver cancer and preparation method thereof | |
| CN102247479B (en) | Antitumor strong medicine and preparation method thereof | |
| CN102861193A (en) | Traditional Chinese medicine composition for treating gastric cancer | |
| CN103933450A (en) | Traditional Chinese medicine composition for preventing or/and treating gastrointestinal diseases and preparation method of composition | |
| CN104069344A (en) | Traditional Chinese medicine composition for treating cancer and preparation and preparation method thereof | |
| CN103463554A (en) | Pharmaceutical composition for treating malignant tumor, preparation method and application | |
| CN108186794B (en) | Traditional Chinese medicine composition for treating lung cancer and preparation method and application thereof | |
| CN106109521A (en) | A kind of Radix Notoginseng oral liquid | |
| CN106038680A (en) | Pharmaceutical composition for treating gastric cancer and preparation method thereof | |
| CN1318034C (en) | Drug prepared by mulberry bark extract | |
| CN104352672A (en) | Tortoise plastron-containing traditional Chinese medicine composition for treating diabetes | |
| CN103989714A (en) | Pharmaceutical composition with antitumor effect and preparation of pharmaceutical composition | |
| CN108283668A (en) | A kind of gastric ulcer composition of American-cockroach-extract-containing and preparation method thereof | |
| CN114796417B (en) | Blood sugar reducing traditional Chinese medicine formula and preparation method thereof | |
| CN110123896B (en) | Pharmaceutical composition for adjuvant treatment of tumor and preparation method thereof | |
| CN113209251A (en) | Traditional Chinese medicine composition, preparation method and application of traditional Chinese medicine composition in preparation of antitumor drugs | |
| CN1931285B (en) | Chinese medicine composition and its preparation process | |
| CN102824526A (en) | Anti-tumor plant extract composition and preparation method and application thereof | |
| CN104784228A (en) | Traditional Chinese medicine for alleviating toxicity of arsenic trioxide, and effective component thereof | |
| CN104547474A (en) | Application of traditional Chinese medicine preparation used for preparing medicine for treating liver cancer | |
| CN104398627A (en) | Esophagus cancer medicament with poison for combating poison and preparation method of esophagus cancer medicament | |
| CN104758749A (en) | Traditional Chinese medicine composition for treating liver cancer |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20161026 |
|
| RJ01 | Rejection of invention patent application after publication |