CN106038615A - Extraction technology of active substances in Thelephora ganbajun Zang and application of active substances in Thelephora ganbajun Zang - Google Patents
Extraction technology of active substances in Thelephora ganbajun Zang and application of active substances in Thelephora ganbajun Zang Download PDFInfo
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- CN106038615A CN106038615A CN201610400704.7A CN201610400704A CN106038615A CN 106038615 A CN106038615 A CN 106038615A CN 201610400704 A CN201610400704 A CN 201610400704A CN 106038615 A CN106038615 A CN 106038615A
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- ganbajun
- extraction process
- active substance
- extracting solution
- active substances
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/06—Fungi, e.g. yeasts
- A61K36/07—Basidiomycota, e.g. Cryptococcus
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
Abstract
The invention discloses an extraction technology of active substances in Thelephora ganbajun Zang. The extraction technology is simple, convenient and fast, is applicable to large-scale industrial production and can keep activity of chemical components in the Thelephora ganbajun Zang to the greatest extent. With the adoption of the chemical active substances extracted with the method, cancer cell growth can be inhibited remarkably, and good anti-oxidative and anti-bacterial effects are realized.
Description
Technical field
The present invention relates to the extraction process of a kind of active substance, particularly to the extraction process of active substance in GANBAJUN.
Background technology
GANBAJUN is the distinctive rare wild edible fungus in Yunnan Province, also cries flower bacterium, gingival cyst of mucous gland in the newborn bacterium.It is grown in the middle regions of the Yunnan Province and Yunnan
Between the mountain forest pinaster in west, originate in seven rainy seasons in August, the most unrealized artificial culture.When GANBAJUN is unearthed, in yellowish-brown, aging
Time, become pitchy.GANBAJUN contains abundant calcium, ferrum, protein, thiamine, has the highest nutritive value.
At present, applied research to GANBAJUN focuses primarily upon the strain separating of GANBAJUN, promotees numerous expanding propagation, fermentation both at home and abroad
Cultivate and contain the preparation of mycelium flavouring agent.But the extraction about GANBAJUN active component is studied and applies nearly no report
Road.It is true that GANBAJUN has critically important food therapy value: such as, GANBAJUN contains abundant antioxidant, can remove people
Body free radical, slow down aging；Containing abundant antibiotic substance, it is possible to the generation of suppression inflammation；There is anticancer ability etc..
Although at present conventional dipping and soxhlet extraction method can extract the active substance obtaining in GANBAJUN, but these
Method extraction efficiency is relatively low, and extraction process is complicated, and elapsed time is longer, and output is relatively low, and is easily destroyed the work of extraction of substance
Property.Therefore, a kind of method tool that can extract active substance in GANBAJUN to greatest extent is found to be of great significance.
Summary of the invention
It is an object of the invention to provide the extraction process of a kind of simple, fast GANBAJUN active substance.
The technical solution used in the present invention is:
In a kind of GANBAJUN, the extraction process of active substance, comprises the steps of
1) after GANBAJUN fully being dried, physical pulverization；
2) GANBAJUN after pulverizing adds extracting solution, fully mixes；
3) after ultrasonic water bath processes, centrifugal collection supernatant.
As preferably: described in be crushed to 25～150 mesh.
As preferably: described extracting solution is ethanol water, wherein ethanol content (v/v) is 40%～80%.
As preferably: described GANBAJUN is 1:5～70(g/mL with the mass volume ratio of extracting solution).
As preferably: described bath temperature is 30～75 DEG C.
As preferably: after extraction process also includes fully mixing extracting solution and GANBAJUN, be-20～-40 in temperature
4～8h are stood under the conditions of DEG C.
Above-described extraction process extracts the GANBAJUN active substance obtained application in preparing anti-tumor agent.
Above-described extraction process extracts active substance application in preparing anti-oxidation preparation in the GANBAJUN obtained.
Above-described extraction process extracts active substance application in preparing antibiotic preparation in the GANBAJUN obtained.
This simple and convenient extraction, quick, it is adaptable to large-scale commercial production, and can farthest keep GANBAJUN
The activity of middle bioactive compound, has breakthrough progress.
Detailed description of the invention
Embodiment 1, the extraction I of GANBAJUN active substance
Fresh food bacterium removes the impurity on surface with clean plastic blade, dries to constant weight, pulverizes with cooking machine after going the removal of impurity
Being 100 mesh, weigh 3g mushroom sample, add 70.15mL extracting solution, extracting solution is 57.38%(v/v) ethanol solution.At-30 DEG C
After K cryogenic treatment 4h, ultrasonic 10min under the conditions of 40 DEG C, supersonic frequency is 500w.5000r is centrifuged 30 min, collects supernatant
Liquid.Being evaporated under the conditions of 40 DEG C by supernatant, the quality weighing the active substance obtained is 0.76 g.
Embodiment 2, the extraction II of GANBAJUN active substance
Fresh food bacterium removes the impurity on surface with clean plastic blade, dries to constant weight, pulverizes with cooking machine after going the removal of impurity
Being 100 mesh, weigh 3g mushroom sample, add 70.15mL extracting solution, extracting solution is 57.38%(v/v) ethanol solution.At-20 DEG C
After K cryogenic treatment 6h, ultrasonic 10min under the conditions of 40 DEG C, supersonic frequency is 500w.5000r is centrifuged 30 min, collects supernatant
Liquid.Being evaporated under the conditions of 40 DEG C by supernatant, the quality weighing the active substance obtained is 0.84 g.
Embodiment 3, the extraction III of GANBAJUN active substance
Fresh food bacterium removes the impurity on surface with clean plastic blade, dries to constant weight, pulverizes with cooking machine after going the removal of impurity
It is 25 mesh, precise 3g GANBAJUN, add 120mL extracting solution, extracting solution is 40%(v/v) ethanol solution.At-20 DEG C of low temperature
After processing 6h, ultrasonic 15min under the conditions of 75 DEG C, supersonic frequency is 550w.5000r is centrifuged 30 min, collects supernatant.Will
Supernatant is evaporated under the conditions of 40 DEG C, and the quality weighing the active substance obtained is 0.72 g.
Embodiment 4, the extraction IV of GANBAJUN active substance
Fresh food bacterium removes the impurity on surface with clean plastic blade, dries to constant weight, pulverizes with cooking machine after going the removal of impurity
It is 25 mesh, precise 3g GANBAJUN, add 15mL extracting solution, extracting solution is 40%(v/v) ethanol solution.At-25 DEG C of low temperature
After reason 6h, ultrasonic 15min under the conditions of 75 DEG C, supersonic frequency is 550w.5000r is centrifuged 30 min, collects supernatant.By upper
Clear liquid is evaporated under the conditions of 40 DEG C, and the quality weighing the active substance obtained is 0.68g.
Embodiment 5, the extraction V of GANBAJUN active substance
Fresh food bacterium removes the impurity on surface with clean plastic blade, dries to constant weight, pulverizes with cooking machine after going the removal of impurity
It is 50 mesh, precise 3g GANBAJUN, add 160mL extracting solution, extracting solution is 50%(v/v) ethanol solution.At-40 DEG C of low temperature
After processing 5h, ultrasonic 8min under the conditions of 30 DEG C, supersonic frequency is 600w.5000r is centrifuged 30 min, collects supernatant.By upper
Clear liquid is evaporated under the conditions of 40 DEG C, and the quality weighing the active substance obtained is 0.42g.
Embodiment 6, the extraction VI of GANBAJUN active substance
Fresh food bacterium removes the impurity on surface with clean plastic blade, dries to constant weight, pulverizes with cooking machine after going the removal of impurity
It is 50 mesh, precise 3g GANBAJUN, add 160mL extracting solution, extracting solution is 50%(v/v) ethanol solution.At-35 DEG C of low temperature
After processing 4h, ultrasonic 8min under the conditions of 30 DEG C, supersonic frequency is 600w.5000r is centrifuged 30 min, collects supernatant.By upper
Clear liquid is evaporated under the conditions of 40 DEG C, and the quality weighing the active substance obtained is 0.66g.
Embodiment 7, the extraction VII of GANBAJUN active substance
Fresh food bacterium removes the impurity on surface with clean plastic blade, dries to constant weight, pulverizes with cooking machine after going the removal of impurity
It is 150 mesh, precise 3g GANBAJUN, add 210mL extracting solution, extracting solution is 80%(v/v) ethanol solution.At-35 DEG C of low temperature
After processing 4h, ultrasonic 20min under the conditions of 60 DEG C, supersonic frequency is 800w.5000r is centrifuged 30 min, collects supernatant.Will
Supernatant is evaporated under the conditions of 40 DEG C, and the quality weighing the active substance obtained is 0.72g.
Embodiment 8, the extraction VIII of GANBAJUN active substance
Fresh food bacterium removes the impurity on surface with clean plastic blade, dries to constant weight, pulverizes with cooking machine after going the removal of impurity
It is 150 mesh, precise 3g GANBAJUN, add 210mL extracting solution, extracting solution is 80%(v/v) ethanol solution.At-40 DEG C of low temperature
After processing 8h, ultrasonic 20min under the conditions of 60 DEG C, ultrasonic power is 800w.5000r is centrifuged 30 min, collects supernatant.By supernatant
Liquid is evaporated under the conditions of 40 DEG C, and the quality weighing the active substance obtained is 0.64g.
Embodiment 9, the antibacterial effect of GANBAJUN active substance
The GANBAJUN active substance 10mg prepared in each Example 1～8, detection GANBAJUN active substance is to large intestine respectively
The antibacterial effect of four kinds of antibacterials such as bacillus, moscow' paratyphi B, Bacillus cereus, staphylococcus aureus.Specifically
Detection method is: according to micro-broth dilution method, add in 96 microwell plates prepare in advance bacteria suspension, MH meat soup, sample, make
Obtaining the bacterial concentration in each hole identical, growth control is made up of deionized water, MH meat soup and bacteria suspension.Afterwards, by 96 micropores
Plate is placed on vortex mixer mixing.Put into microplate reader, under the conditions of 630nm, read absorbance before cultivating.Then, 37 DEG C are put it into
Calorstat is cultivated 21 hours.Finally, the absorbance (630nm) after cultivating is read.Calculate antibacterial by absorbance change value
Rate.Obtain result as shown in table 1:
The antibacterial effect (%) of table 1 GANBAJUN active substance
Embodiment 10, the anticancer effect of GANBAJUN active substance
The GANBAJUN active substance 10mg prepared in each Example 1～8, uses mtt assay to detect GANBAJUN activity respectively
Material is raw to A549 lung carcinoma cell, HepG2 hepatoma carcinoma cell, MCF-7 breast cancer cell and four kinds of cancerous cell of HT-29 colorectal cancer cells
Long impact.Obtain result as shown in table 2:
The anticancer effect (* 100%) of table 2 GANBAJUN active substance
Embodiment 11, the antioxidant effect of GANBAJUN active substance
GANBAJUN active substance 10 mg prepared in each Example 1～8, uses ABTS radical scavenging activity to measure
Method measures the antioxidant effect of the GANBAJUN active substance extracted, and obtains result as shown in table 3:
The antioxidant effect (μm ol Trolox/g) of table 3 GANBAJUN active substance
Above example only introduces the preferred case of the present invention, to those skilled in the art, without departing substantially from the present invention
Any obvious changes and improvements carried out in the scope of spirit, are regarded as the part of the present invention.
Claims (9)
1. an extraction process for active substance in GANBAJUN, comprises the steps of
1) after GANBAJUN fully being dried, physical pulverization；
2) GANBAJUN after pulverizing adds extracting solution, fully mixes；
3) after ultrasonic water bath processes, centrifugal collection supernatant.
Extraction process the most according to claim 1, it is characterised in that be crushed to 25～150 mesh described in:.
Extraction process the most according to claim 1, it is characterised in that: described extracting solution is ethanol water, wherein ethanol
Content (v/v) is 40%～80%.
Extraction process the most according to claim 1, it is characterised in that: described GANBAJUN with the mass volume ratio of extracting solution is
1:5～70(g/mL).
Extraction process the most according to claim 1, it is characterised in that: described bath temperature is 30～75 DEG C.
Extraction process the most according to claim 1, it is characterised in that: extraction process also includes filling extracting solution with GANBAJUN
Point mixing after, temperature be-20～-40 DEG C under the conditions of standing 4～8h.
7. during the extraction process according to any one of claim 1～6 extracts the GANBAJUN obtained, active substance is anti-swollen in preparation
Application in tumor preparation.
8. during the extraction process described in any one of claim 1～6 extracts the GANBAJUN obtained, active substance is preparing antioxidation
Application in preparation.
9. during the extraction process described in any one of claim 1～6 extracts the GANBAJUN obtained, active substance is preparing antibacterial system
Application in agent.
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| CN201610400704.7A CN106038615A (en) | 2016-06-08 | 2016-06-08 | Extraction technology of active substances in Thelephora ganbajun Zang and application of active substances in Thelephora ganbajun Zang |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106727736A (en) * | 2016-11-30 | 2017-05-31 | 中山大学 | The preparation method and applications of Boletus luridus active material |
| CN107281011A (en) * | 2017-07-03 | 2017-10-24 | 中国药科大学 | Edible fungi root extract and the mouthwash using its preparation |
| CN110305758A (en) * | 2019-05-10 | 2019-10-08 | 王少龙 | A kind of production method that wizened bacterium is steep in wine |
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| CN102210449A (en) * | 2011-06-23 | 2011-10-12 | 张鹏 | Liquid submerged fermentation culture of Thelephora ganbajun Zang and preparation methods of seasoning and selenium-enriched product of Thelephora ganbajun Zan |
| CN103719833A (en) * | 2014-01-10 | 2014-04-16 | 申洁 | Selenium-enriched sparassis crispa powder as well as food preparation method and preparation |
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| CN102210449A (en) * | 2011-06-23 | 2011-10-12 | 张鹏 | Liquid submerged fermentation culture of Thelephora ganbajun Zang and preparation methods of seasoning and selenium-enriched product of Thelephora ganbajun Zan |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106727736A (en) * | 2016-11-30 | 2017-05-31 | 中山大学 | The preparation method and applications of Boletus luridus active material |
| CN106727736B (en) * | 2016-11-30 | 2020-05-19 | 中山大学 | Preparation method and application of active substance of boletus flavus |
| CN107281011A (en) * | 2017-07-03 | 2017-10-24 | 中国药科大学 | Edible fungi root extract and the mouthwash using its preparation |
| CN107281011B (en) * | 2017-07-03 | 2020-02-11 | 中国药科大学 | Edible fungus mycorrhiza extract and mouthwash prepared from same |
| CN110305758A (en) * | 2019-05-10 | 2019-10-08 | 王少龙 | A kind of production method that wizened bacterium is steep in wine |
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Application publication date: 20161026 |