CN106867913A - A kind of liquid fermentation method of selenium-enriched hericium erinaceus - Google Patents
A kind of liquid fermentation method of selenium-enriched hericium erinaceus Download PDFInfo
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- CN106867913A CN106867913A CN201611191666.5A CN201611191666A CN106867913A CN 106867913 A CN106867913 A CN 106867913A CN 201611191666 A CN201611191666 A CN 201611191666A CN 106867913 A CN106867913 A CN 106867913A
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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Abstract
A kind of liquid fermentation method of selenium-enriched hericium erinaceus, is prepared by Hericium erinaceus seed culture medium；Hericium erinaceus fermentation stage 1；Hericium erinaceus fermentation stage 2；Hericium erinaceus fermentation stage 3；Hericium erinaceus fermentation stage 4；It is centrifuged, dries, through collected after centrifugation mycelium, the mycelium being collected into is placed in 65 DEG C to zymotic fluid, biodiversity number is less than 5% in heated-air circulation oven drying to mycelium, you can obtains the selenium-enriched hericium erinaceus mycelium that Se content is 0.5mg/kg.The mycelial zymotechnique of a kind of selenium-enriched hericium erinaceus that the present invention is obtained by the optimization of multistage fermentation condition, while adding selenium source to obtain selenium-enriched hericium erinaceus during the fermentation, nutritive value is higher, meanwhile, rational technology has safely good commercial promise.
Description
Technical field
The present invention relates to selenium-enriched hericium erinaceus development field, more particularly to a kind of liquid fermentation method of selenium-enriched hericium erinaceus.
Background technology
Hericium erinaceus is the rare one kind of comparing inside mushroom vegetables, is also a kind of rare edible mushroom, is put into eight big
One of mountain delicacy.Hericium erinaceus have use effect well, with nutrition and medicinal combination.Gu has " hedgehog hydnum, seafood delights swallow in mountain
Nest " is said.Hedgehog hydnum and shark's fin, bear's paw, bird's nest are simultaneously described as four big famous dishes.Hedgehog hydnum is a kind of large-scale meat bacterium in remote, thickly forested mountains,
Like being grown in broad-leaved trunk section or treehole.It is white when immature, then become brushy yellowish-brown after maturation, from shape and color
On see head like hair monkey, so gain the name.Hericium erinaceus is the fructification of tooth bacterium section plant hedgehog hydnum.It is China North China, northeast, Central-South
It is distributed with Sichuan, Yunnan, Gansu, zhejiang and other places, summer and autumn harvesting.
The nutritional ingredient of Hericium erinaceus is very high, contains 26.3 grams of protein in dry product per hectogram, is two times of mushroom.It contains ammonia
Base acid up to 17 kinds, wherein needed by human body accounts for 8 kinds.It is qualified high protein, low per fatty 4.2 grams of hectogram hedgehog hydnum
Fatty foodstuff, is also rich in various vitamins and inorganic salts in addition.Hericium erinaceus improves a poor appetite, and strengthens barrier of gastric mucosa function, carries
The effects such as lymphocyte transformation rate high, lifting leucocyte.Therefore human body can be made to improve the immunocompetence to disease.Hericium erinaceus is still
Good nourishing food, has good efficacy to neurasthenia, digestive tract ulcer.In screening anticancer medicine, find its to skin,
Muscle cancerous swelling has obvious anticancer function.
Traditional hericium erinaceus fruiting body artificial culture needs to treat cell age to more than 3 months, is removed when fructification grows up to, dries
Or using fresh herb.Modern study show artificial fermentation's hericium mycelium similar with hericium erinaceus fruiting body composition, active component content and
There was no significant difference for pharmacological action.Artificial fermentation's hericium mycelium easily realizes scale and with short production cycle, production
Low cost, can be used as the preferable substitute of hericium erinaceus fruiting body.
The content of the invention
To solve the problems, such as above-mentioned prior art, it is an object of the invention to provide a kind of liquid of selenium-enriched hericium erinaceus
Fermentation process
To reach above-mentioned purpose, the technical scheme is that：
A kind of liquid fermentation method of selenium-enriched hericium erinaceus, is made up of following steps：
It is prepared by step one, Hericium erinaceus seed culture medium：
By 10~15g/L of kudzu-vine root powder, 1~2g/L of Se-enriched yeast, glucose 20g/L, pH 5.0, KH2PO40.2~0.5g/
L, MgSO40.75~1g/L；Liquid seed culture medium is prepared, triangular flask is distributed into, 1L/ triangular flasks liquid amount is 400ml,
125 DEG C of sterilizing 30min, after accessing Hericium erinaceus solid seed after cooling, insert isothermal vibration shaking table, concussion and cultivate temperature 24~26
DEG C, incubation time 7 days obtains Hericium erinaceus liquid seeds standby with this understanding；
Step 2, Hericium erinaceus fermentation stage 1
Liquid fermentation medium formula is：10~15g/L of soy bean protein hydrolysate, 0.2~0.5g/L of Se-enriched yeast, glucose
20g/L, pH 5.0, KH2PO40.2~0.5g/L, MgSO40.75~1g/L；Culture medium is prepared, loads fermentation jar, 125
DEG C sterilizing 30min, after cooling access Hericium erinaceus solid seed after, aerobic fementation, throughput 1:1 (V/V), cultivation temperature 24~26
DEG C, selenium source is added after incubation time 48h, the sterilized selenium sources of 50ml are added in every liter of zymotic fluid；
Step 3, Hericium erinaceus fermentation stage 2
After selenium source being added under aseptic condition in step 2 zymotic fluid, fermentation tank continuation aerobic fementation, cultivation temperature 24~
26 DEG C, incubation time 48h；
Step 4, Hericium erinaceus fermentation stage 3
To continue to add selenium source, magnesium sulfate, vitamin B compound under aseptic condition in step 3 fermentation ends after fermentation liquid,
The sterilized selenium sources of 50ml, 0.5g magnesium sulfate and 0.5g vitamin B compounds are added in every liter of zymotic fluid；After stirring, continue
Aerobic fementation, throughput 1:1 (V/V), 24~26 DEG C of cultivation temperature, incubation time 48h；
Step 5, Hericium erinaceus fermentation stage 4
After fermentation stage 3 terminates, fermentation tank throughput is reduced to 1:0.5 (V/V), cultivation temperature is set as 28 DEG C, training
Go out tank after supporting time 24h；
Step 6, centrifugation, drying
Through collected after centrifugation mycelium, the mycelium being collected into is placed in 65 DEG C, heated-air circulation oven drying to bacterium to zymotic fluid
Biodiversity number is less than 5% in filament, you can obtain the selenium-enriched hericium erinaceus mycelium that Se content is 0.5mg/kg.
Further, in the step one, kudzu-vine root powder is that the Pueraria lobota of 100 mesh sieves was directly crushed after the artificial cultivation root of kudzu vine is dried
Root powder.
Further, in the step one and step 2, Se-enriched yeast selenium content is 2000ppm.
Further, the selenium source is prepared as：5~10g of vitamin C, 20~50g of soy bean protein hydrolysate, sodium selenite
1~3g, pure water 1L, after each raw material mixing, stands more than 8h, and selenium source is after being processed through ultra-high pressure sterilization.
Further, in gained selenium-enriched hericium erinaceus, Se content is 0.5mg/kg.
Relative to prior art, beneficial effects of the present invention are：
The mycelial fermentation work of a kind of selenium-enriched hericium erinaceus that patent of the present invention is obtained by the optimization of multistage fermentation condition
Skill, while adding selenium source to obtain selenium-enriched hericium erinaceus during the fermentation, nutritive value is higher, meanwhile, rational technology has safely
Good commercial promise.
Brief description of the drawings
Fig. 1 is a kind of schematic diagram of the liquid fermentation method of selenium-enriched hericium erinaceus of the invention.
Specific embodiment
Technical solution of the present invention is described in further detail with reference to the accompanying drawings and detailed description：
As shown in figure 1,
A kind of liquid fermentation method of selenium-enriched hericium erinaceus, is made up of following steps：
1st, prepared by Hericium erinaceus seed culture medium：
Seed culture based formulas kudzu-vine root powder (the artificial cultivation root of kudzu vine directly crushed 100 mesh sieves after drying) 10~15g/L, richness
Selenium yeast (selenium content 2000ppm) 1~2g/L, glucose 20g/L, pH 5.0, KH2PO4 0.2~0.5g/L, MgSO40.75
~1g/L.Liquid seed culture medium is prepared, triangular flask is distributed into, 1L/ triangular flasks liquid amount is 400ml, 125 DEG C of sterilizings
30min, after accessing Hericium erinaceus solid seed after cooling, inserts isothermal vibration shaking table, 24~26 DEG C of concussion and cultivate temperature, during culture
Between 7 days, Hericium erinaceus liquid seeds are obtained with this understanding.
2nd, Hericium erinaceus fermentation stage 1
10~15g/L of liquid fermentation medium formula soy bean protein hydrolysate, Se-enriched yeast (selenium content 2000ppm) 0.2~
0.5g/L, glucose 20g/L, pH 5.0, KH2PO4 0.2~0.5g/L, MgSO40.75~1g/L.Culture medium is prepared,
Loading fermentation jar, 125 DEG C of sterilizing 30min, after accessing Hericium erinaceus solid seed after cooling, aerobic fementation, throughput 1:1(V/
V), 24~26 DEG C of cultivation temperature, add selenium source after incubation time 48h, the sterilized selenium sources of 50ml are added in every liter of zymotic fluid.
The preparation of selenium source：5~10g of vitamin C, 20~50g of soy bean protein hydrolysate, 1~3g of sodium selenite, pure water 1L,
After each raw material mixing, more than 8h is stood, selenium source is after being processed through ultra-high pressure sterilization.
3rd, Hericium erinaceus fermentation stage 2
After adding selenium source under aseptic condition in step 2 zymotic fluid, fermentation tank continues aerobic fementation, cultivation temperature 24~26
DEG C, incubation time 48h.
4th, Hericium erinaceus fermentation stage 3
Continue to add selenium source, magnesium sulfate, vitamin B compound, every liter under aseptic condition in step 3 fermentation ends after fermentation liquid
The sterilized selenium sources of 50ml, 0.5g magnesium sulfate and 0.5g vitamin B compounds are added in zymotic fluid.After stirring, continue to ventilate
Fermentation, throughput 1:1 (V/V), 24~26 DEG C of cultivation temperature, incubation time 48h.
4th, Hericium erinaceus fermentation stage 4
After fermentation stage 3 terminates, fermentation tank throughput is reduced to 1:0.5 (V/V), cultivation temperature is set as 28 DEG C, training
Go out tank after supporting time 24h.
5th, it is centrifuged, dries
Through collected after centrifugation mycelium, the mycelium being collected into is placed in 65 DEG C, heated-air circulation oven drying to bacterium to zymotic fluid
Biodiversity number is less than 5% in filament, you can obtain the selenium-enriched hericium erinaceus mycelium that Se content is 0.5mg/kg.
Experimental example：The inventive method products obtained therefrom is carried out into content analysis with tradition aspect product：
Note：Traditional zymotic is a certain amount of sodium selenite directly to be added in the liquid fermentation medium of Hericium erinaceus (selenium is total
Amount is identical with the amount of the total selenium element of patent of the present invention), fermentation obtains selenium-enriched hericium erinaceus mycelium.
First, in product selenium and Zn-ef ficiency form and content analysis
Using 700 high-resolution of ContrAA-continuum source atomic absorption spectrometer (H R-CS AAS)；XT-9900 type intelligence
Energy microwave dissolver is analyzed to Selenium In Some Selenium-rich Biological Samples and Zn-ef ficiency.
Sample is placed in dry polytetrafluoroethylene (PTFE) micro-wave diminishing pot to be cleared up, while in kind doing blank.Root
According to the height of surveyed metallic element concentration, the dilution of suitable multiple is carried out to it with 0.5%HNO3 (V/V) solution, then used again
HR-CS FAAS are measured.Experiment selection D101, XAD-2 and LSA-10 this 3 kinds of macroreticular resins separate organic in soluble states
And nothing
Machine state.
The content and morphological analysis of Selenium In Some Selenium-rich Biological Samples and zinc
The content of zinc and selenium in the sample of patent of the present invention is can be seen that by the experimental result in table compared with traditional fermentation process
Content is higher in mycelium, and patent Selenium In Some Selenium-rich Biological Samples of the present invention are main with organic presence, absorption of human body are also beneficial to, to machine
Body is more beneficial.
Separately, the content analysis for carrying out polysaccharide in mycelium according to sulfuric acid anthrone method find that patented product polysaccharide of the present invention contains
Amount 27.68mg/kg relatively compares 19.55mg/kg and improves 41.58%.
In summary result of study finds, patented technology of the present invention, no matter from the Se content and fractions distribution of product, or
Have compared with quantum jump compared with traditional zymotic from the point of view of the content of polysaccharide, with certain advance.
The above, specific embodiment only of the invention, but protection scope of the present invention is not limited thereto, and it is any
The change or replacement expected without creative work, should all be included within the scope of the present invention.Therefore, it is of the invention
Protection domain should be determined by the scope of protection defined in the claims.
Claims (5)
1. a kind of liquid fermentation method of selenium-enriched hericium erinaceus, it is characterised in that be made up of following steps：
It is prepared by step one, Hericium erinaceus seed culture medium：
By 10~15g/L of kudzu-vine root powder, 1~2g/L of Se-enriched yeast, glucose 20g/L, pH 5.0, KH2PO40.2~0.5g/L,
MgSO40.75~1g/L；Liquid seed culture medium is prepared, triangular flask is distributed into, 1L/ triangular flasks liquid amount is 400ml,
125 DEG C of sterilizing 30min, after accessing Hericium erinaceus solid seed after cooling, insert isothermal vibration shaking table, concussion and cultivate temperature 24~26
DEG C, incubation time 7 days obtains Hericium erinaceus liquid seeds standby with this understanding；
Step 2, Hericium erinaceus fermentation stage 1
Liquid fermentation medium formula is：10~15g/L of soy bean protein hydrolysate, 0.2~0.5g/L of Se-enriched yeast, glucose 20g/
L, pH 5.0, KH2PO40.2~0.5g/L, MgSO40.75~1g/L；Culture medium is prepared, loads fermentation jar, 125 DEG C
Sterilizing 30min, after accessing Hericium erinaceus solid seed after cooling, aerobic fementation, throughput 1:1 (V/V), cultivation temperature 24~26
DEG C, selenium source is added after incubation time 48h, the sterilized selenium sources of 50ml are added in every liter of zymotic fluid；
Step 3, Hericium erinaceus fermentation stage 2
After selenium source being added under aseptic condition in step 2 zymotic fluid, fermentation tank continuation aerobic fementation, 24~26 DEG C of cultivation temperature,
Incubation time 48h；
Step 4, Hericium erinaceus fermentation stage 3
To continue to add selenium source, magnesium sulfate, vitamin B compound, every liter under aseptic condition in step 3 fermentation ends after fermentation liquid
The sterilized selenium sources of 50ml, 0.5g magnesium sulfate and 0.5g vitamin B compounds are added in zymotic fluid；After stirring, continue to ventilate
Fermentation, throughput 1:1 (V/V), 24~26 DEG C of cultivation temperature, incubation time 48h；
Step 5, Hericium erinaceus fermentation stage 4
After fermentation stage 3 terminates, fermentation tank throughput is reduced to 1:0.5 (V/V), cultivation temperature is set as 28 DEG C, during culture
Between go out tank after 24h；
Step 6, centrifugation, drying
Through collected after centrifugation mycelium, the mycelium being collected into is placed in 65 DEG C, heated-air circulation oven drying to mycelium to zymotic fluid
Middle biodiversity number is less than 5%, you can obtain selenium-enriched hericium erinaceus mycelium.
2. method according to claim 1, it is characterised in that in the step one, kudzu-vine root powder is dry for the artificial cultivation root of kudzu vine
The kudzu-vine root powder of 100 mesh sieves was directly crushed after dry.
3. method according to claim 1, it is characterised in that in the step one and step 2, Se-enriched yeast selenium content
It is 2000ppm.
4. method according to claim 1, it is characterised in that selenium source is prepared as in the step 2：Vitamin C 5~
10g, 20~50g of soy bean protein hydrolysate, 1~3g of sodium selenite, pure water 1L, after each raw material mixing, stand more than 8h, through superelevation
Selenium source is after pressure sterilization treatment.
5. method according to claim 1, it is characterised in that in gained selenium-enriched hericium erinaceus, Se content is 0.5mg/kg.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108265011A (en) * | 2018-04-26 | 2018-07-10 | 杭州雪域生物技术有限公司 | Mycelium liquid fermentation medium composition of a kind of rich-selenium Agaricus blazei and preparation method thereof and fermentation process |
| CN108546174A (en) * | 2018-07-11 | 2018-09-18 | 广东泓睿科技有限公司 | A kind of selenium-enriched edible mushroom culture medium and preparation method thereof |
| CN109337895A (en) * | 2018-11-27 | 2019-02-15 | 江苏高航农业科技有限公司 | A kind of production method of good quality and high output selenium-enriched hericium erinaceus strain |
| CN112120132A (en) * | 2020-09-09 | 2020-12-25 | 湖北民族大学 | Selenium-rich hericium erinaceus mycelium beverage and preparation method thereof |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN108265011A (en) * | 2018-04-26 | 2018-07-10 | 杭州雪域生物技术有限公司 | Mycelium liquid fermentation medium composition of a kind of rich-selenium Agaricus blazei and preparation method thereof and fermentation process |
| CN108546174A (en) * | 2018-07-11 | 2018-09-18 | 广东泓睿科技有限公司 | A kind of selenium-enriched edible mushroom culture medium and preparation method thereof |
| CN109337895A (en) * | 2018-11-27 | 2019-02-15 | 江苏高航农业科技有限公司 | A kind of production method of good quality and high output selenium-enriched hericium erinaceus strain |
| CN109337895B (en) * | 2018-11-27 | 2021-07-27 | 上海紫京科贸有限公司 | Production method of high-quality high-yield selenium-rich hericium erinaceus strain |
| CN112120132A (en) * | 2020-09-09 | 2020-12-25 | 湖北民族大学 | Selenium-rich hericium erinaceus mycelium beverage and preparation method thereof |
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Application publication date: 20170620 |