CN106035054B - A kind of breeding method for improving rice big grain kind rice transparency - Google Patents
A kind of breeding method for improving rice big grain kind rice transparency Download PDFInfo
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- CN106035054B CN106035054B CN201610210675.8A CN201610210675A CN106035054B CN 106035054 B CN106035054 B CN 106035054B CN 201610210675 A CN201610210675 A CN 201610210675A CN 106035054 B CN106035054 B CN 106035054B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/02—Methods or apparatus for hybridisation; Artificial pollination ; Fertility
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/04—Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection
Abstract
The invention discloses a kind of breeding method for improving rice big grain kind rice transparency; by from the double grains gene for being different from normally arriving paddy; by baclccrossing techniques by double grains channel genes into big grain kind SLG; the big grain primary characteristics of SLG are kept, a SLG paddy is coated with a rice and is changed into paddy 2 rice of coating to improve rice transparency；From the double grains gene different from normal paddy, it is imported into by baclccrossing techniques in the big grain kind SLG of the big poor transparency of grain grain type, the problem of improving big grain kind core white big poor transparency；By being repeatedly returned, with reference to M8003 line by double grains channel genes into SLG, be bred as the SLG double grains that greatly improve of transparency.The transparency of the SLG double grains rice of the present invention has larger significantly improve compared with SLG；SLG double grains both keep SLG big grain characteristic, can solve the problems, such as the excessive poor transparency of SLG core whites again compared with SLG.
Description
Technical field
The invention belongs to crop breeding technical field, more particularly to a kind of breeding side for improving rice transparency
Method.
Background technology
Paddy rice is mutually hooked by two edge of inside and outside bran and closes the husk for forming closing, and the inside is coated with a brown rice.Glumelle
Played an important role with the size of lemma in Grain formation, on the one hand synthesize assimilation quotient, on the other hand seed is moulded
Shape, influence rice figure and Grain filling etc. and then influence the transparency of rice.Rice transparency is to influence rice quality
Important evaluation index, rice transparency directly affect Appearance Quality of Paddy Rice.In recent years, it is continuous with our people's living standard
Improve, the rice that transparency is good, quality is excellent is increasingly welcome by the people.The development of China's rice experienced with transition to be compared several times
Larger change, it is concentrated mainly in plant type and fringe type.The rice varieties that last century the fifties are bred as all are long-stalked varieties, point
Tiller power is weaker, and loosely, lodging resistance is poor for plant type, and yield is relatively low, exterior quality poor transparency；The sixties have been bred as upright close
Fringe type japonica rice variety " sweet osmanthus yellow ", although yield is higher, exterior quality transparency or poor；The early 1990s Jiaxing City agriculture
Institute of section has been bred as elegant water 63, overcome conventional rice varieties generally existing rice transparency it is poor the shortcomings that, in transparency and
Obvious improvement is obtained on Cooking Quality, is widely applied in production.With the change of cropping system, elegant water 63
One-season Planting because the problem of plant is higher, stalk is soft, resistance is not strong, and lodging resistance is poor, gradually shows, production on there is an urgent need to
The new rice variety that yield is higher, resistance is stronger, meter Zhi Geng is excellent substitutes elegant water 63.Jiaxing City academy of agricultural sciences's last century Mo and this century
Just select with Jiahua-1 and elegant water 110, elegant water 09 and elegant water 134 " source of semidwarf supplies the storehouse of dense cluster type " for representative
Novel strain type conventional Rice fine quality, not only the good food flavor of transparency is good, and yield, which has, to be substantially improved from average yield per mu from 500 kilograms
Bring up to 600 kilograms.But on the whole, product of improvement of these breeding methods to transparency just for 25 grams or so of mass of 1000 kernel
Kind, and limited for kind of the mass of 1000 kernel more than 30 grams by filling rate, improve limited extent.
Existing big grain kind SLG mass of 1000 kernel is up to 45 grams of excessive poor transparencies of core white.
The content of the invention
It is an object of the invention to provide a kind of breeding method for improving rice big grain kind rice transparency, it is intended to solves
The problem of existing SLG (preserving number CN16466) rice core white excessive poor transparency.
The present invention is achieved in that a kind of breeding method for improving rice big grain kind rice transparency, the raising
The breeding method of rice transparency is by from being different from the normal double grains gene for arriving paddy, by baclccrossing techniques by double grains
Channel genes keep the big grain primary characteristics of SLG into big grain kind SLG, a SLG paddy is coated with a rice and are changed into
One paddy is coated with 2 rice to improve rice transparency.
Further, the breeding method for improving rice transparency comprises the following steps：
Step 1, the Zhejiang round-grained rice 22- double grains of two rice are coated with using every grain and big grain kind SLG hybridizes, F1Use SLG
Backcrossing obtains BC1F1；
Step 2, plant BC1F1Mixed receipts obtain BC1F2Segregating population, BC1F2Segregating population field planting, is returned with SLG
To BC2F1, leaving transparency, good cross combination continues to plant BC2F1BC is returned to obtain with SLG3F1；
Step 3, plant BC3F1Mixed receipts obtain BC3F2Segregating population, BC3F2Segregating population field planting, is returned with SLG
To BC4F1, leaving transparency, good cross combination continues to plant BC4F1BC is returned to obtain with SLG5F1；
Step 4, plant BC5F1Mixed receipts obtain BC5F2Segregating population, BC5F2Segregating population field planting, leaves transparency
Good double grains strain is SLG- double grains.
Further, the BC1F2500 plants of segregating population field planting, select the close band double grains characteristic of same SLG plant types grain type
More than 20 plants of strain continue to be returned to obtain BC with SLG as female parent2F1, dried after female parent is ripe and be ground into brown rice observation transparency,
Give up the cross combination of maternal poor transparency.
Further, the BC3F2500 plants of segregating population field planting, select the close band double grains characteristic of same SLG plant types grain type
More than 20 plants of strain continue to be returned to obtain BC with SLG as female parent4F1, dried after female parent is ripe and be ground into brown rice observation transparency,
Give up the cross combination of maternal poor transparency, leaving transparency, good cross combination continues to plant BC4F1It is returned with SLG
BC5F1。
Further, the BC5F2500 plants of segregating population field planting, therefrom select economical character identical with SLG after ripe
A paddy inside be coated with two rice SLG- double grains strain harvest dry be ground into brown rice observation transparency leave it is transparent
The double grains strain spent is SLG- double grains.
The breeding method provided by the invention for improving rice big grain kind rice transparency, by from different from normally arriving
The double grains gene of paddy, by baclccrossing techniques by double grains channel genes into big grain kind SLG, keep the big grain primary characteristics of SLG,
A SLG paddy is coated with a rice and be changed into paddy 2 rice of coating to improve rice transparency；From difference
In the double grains gene of normal paddy, it is imported into the big grain kind SLG of the big poor transparency of grain grain type, is improved by baclccrossing techniques
The problem of big grain kind core white big poor transparency；By being repeatedly returned, with reference to M8003 line by double grains channel genes into SLG,
It is bred as the SLG- double grains that transparency greatly improves.The present invention using the double grains round-grained rice type kind Zhejiang round-grained rice 22- double grains with recessive gene with
Big grain kind SLG hybridization, segregating generation selects the individual plant for having two ovarys inside a paddy, is returned with SLG, common 5-7 times, obtains
SLG- double grains are obtained, specify that the double seeds of rice are controlled by single recessive gene by the genetic research to the double seeds of rice, hybridize,
Backcrossing female parent is ground into the transparency of brown rice observation rice per all drying from generation to generation, selects transparency good and is used for the maternal of double grains gene
SLG is returned, and the transparency of the SLG- double grains rice obtained by continuous 10 generation, 5 backcrossings has larger significantly improve compared with SLG.Pass through
Hybridization and repeatedly backcrossing are ground into brown rice with reference to segregating generation and exterior quality are screened, and select transparency to show double grains characteristic well
Strain to next generation, the SLG- double grains obtained by continuous 10 generation, 5 backcrossings both keep SLG big grain special compared with SLG
Property, can solve the problems, such as that SLG causes the excessive chalkiness degree high grade of transparency difference of core white, SLG- because the larger grouting of grain is insufficient again
Double grains are to be coated with two ovary Grain fillings compared with SLG inside a grain to be significantly improved；Harvest is ripe
SLG- double grains and SLG seeds send the indices that Ministry of Agriculture's rice quality examines inspection center's detection to influence transparency：SLG is same
SLG- double grains, by 88.3, are reduced to 66.3, reduce 22.0 percentage points compared to chalky grain rate；Chalkiness degree is reduced to by 43.1
18.0, reduce 25.0；Transparency has brought up to 1 grade by 4 grades and has improved 3 grades, and transparency is significantly improved.
Brief description of the drawings
Fig. 1 is the breeding method flow chart of raising rice transparency provided in an embodiment of the present invention.
Embodiment
In order to make the purpose , technical scheme and advantage of the present invention be clearer, with reference to embodiments, to the present invention
It is further elaborated.It should be appreciated that the specific embodiments described herein are merely illustrative of the present invention, it is not used to
Limit the present invention.
The application principle of the present invention is explained in detail below in conjunction with the accompanying drawings.
As shown in figure 1, the breeding method of the raising rice transparency of the embodiment of the present invention comprises the following steps：
S101：The Zhejiang round-grained rice 22- double grains of two rice are coated with using every grain and big grain kind SLG hybridizes, F1Returned with SLG
Friendship obtains BC1F1；
S102：Plant BC1F1Mixed receipts obtain BC1F2Segregating population, BC1F2500 plants of segregating population field planting, selection are same
More than 20 plants of the close strain with double grains characteristic of SLG plant type grain types continues to be returned to obtain BC with SLG as female parent2F1, it is maternal ripe
After dry be ground into brown rice observation transparency, give up the cross combination of maternal poor transparency, leave the good cross combination of transparency after
Continuous plantation BC2F1BC is returned to obtain with SLG3F1；
S103：Plant BC3F1Mixed receipts obtain BC3F2Segregating population, BC3F2500 plants of segregating population field planting, selection are same
More than 20 plants of the close strain with double grains characteristic of SLG plant type grain types continues to be returned to obtain BC with SLG as female parent4F1, it is maternal ripe
After dry be ground into brown rice observation transparency, give up the cross combination of maternal poor transparency, leave the good cross combination of transparency after
Continuous plantation BC4F1BC is returned to obtain with SLG5F1；
S104：Plant BC5F1Mixed receipts obtain BC5F2Segregating population, BC5F2500 plants of segregating population field planting, it is ripe after from
Middle selection economical character dries with the strain harvest that two rice SLG- double grains are coated with inside one paddy of SLG identicals to be ground into
Brown rice observation transparency leaves transparency, and good double grains strain is SLG- double grains.
Zhejiang round-grained rice 22- double grains in the embodiment of the present invention are on June 8th, 2007 by Chinese microorganism strain preservation conservator
Meeting common micro-organisms center income preservation, preserving number：CGMCCNO2081.Depositary institution's title：Chinese microorganism strain preservation pipe
Reason committee common micro-organisms center, depositary institution address：Datun Road, Chaoyang District, Beijing City, Chinese Academy of Sciences's microbe research
Institute, preservation date：On June 8th, 2007, deposit number：CGMCC NO.2081, Classification And Nomenclature：Rice Oryza sativa.
The embodiment of the present invention plants SLG- double grains and SLG, and each more than seed 1kg that harvests send Ministry of Agriculture's rice quality to examine inspection
The transparency of measured center detection SLG- double grains has brought up to 1 grade compared to 4 grades of SLG, and transparency is significantly improved.The present invention
The transparency of brown rice observation rice is ground into, the transparency of rice has larger significantly improve compared with SLG.Examined through Ministry of Agriculture's rice quality
The transparency of inspection center's detection SLG- double grains has brought up to 1 grade compared to 4 grades of SLG, and transparency is significantly improved.
The foregoing is merely illustrative of the preferred embodiments of the present invention, is not intended to limit the invention, all essences in the present invention
All any modification, equivalent and improvement made within refreshing and principle etc., should be included in the scope of the protection.
Claims (4)
1. a kind of breeding method for improving rice big grain kind rice transparency, it is characterised in that the raising rice is saturating
The breeding method of lightness is by selecting the double grains gene different from normal paddy, by baclccrossing techniques by double grains channel genes to greatly
In grain kind SLG, the big grain primary characteristics of SLG are kept, a SLG paddy is coated with a rice and is changed into a paddy coating
2 rice improve rice transparency；
The breeding method for improving rice transparency comprises the following steps：
Step 1, the Zhejiang round-grained rice 22- double grains of two rice are coated with using every grain and big grain kind SLG hybridizes, F1It is returned with SLG
Obtain BC1F1；
Step 2, plant BC1F1Mixed receipts obtain BC1F2Segregating population, BC1F2Segregating population field planting, it is returned to obtain with SLG
BC2F1, leaving transparency, good cross combination continues to plant BC2F1BC is returned to obtain with SLG3F1；
Step 3, plant BC3F1Mixed receipts obtain BC3F2Segregating population, BC3F2Segregating population field planting, it is returned to obtain with SLG
BC4F1, leaving transparency, good cross combination continues to plant BC4F1BC is returned to obtain with SLG5F1；
Step 4, plant BC5F1Mixed receipts obtain BC5F2Segregating population, BC5F2Segregating population field planting, it is good to leave transparency
Double grains strain is SLG- double grains.
2. the breeding method of rice big grain kind rice transparency is improved as claimed in claim 1, it is characterised in that described
BC1F2500 plants of segregating population field planting, the same close strain with double grains characteristic of SLG plant types grain type is selected more than 20 plants as mother
This continues to be returned to obtain BC with SLG2F1, dried after female parent is ripe and be ground into brown rice observation transparency, give up the miscellaneous of maternal poor transparency
Hand over combination.
3. the breeding method of rice big grain kind rice transparency is improved as claimed in claim 1, it is characterised in that described
BC3F2500 plants of segregating population field planting, the same close strain with double grains characteristic of SLG plant types grain type is selected more than 20 plants as mother
This continues to be returned to obtain BC with SLG4F1, dried after female parent is ripe and be ground into brown rice observation transparency, give up the miscellaneous of maternal poor transparency
Combination is handed over, leaving transparency, good cross combination continues to plant BC4F1BC is returned to obtain with SLG5F1。
4. the breeding method of rice big grain kind rice transparency is improved as claimed in claim 1, it is characterised in that described
BC5F2500 plants of segregating population field planting, economical character is therefrom selected to be coated with one paddy the inside of SLG identicals after ripe
The strain harvest of two rice SLG- double grains dries to be ground into brown rice observation transparency and leave the good double grains strain of transparency
SLG- double grains.
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| CN108668882A (en) * | 2018-03-29 | 2018-10-19 | 浙江省农业科学院 | A kind of breeding method improving rice mass of 1000 kernel |
| CN109601368A (en) * | 2018-12-18 | 2019-04-12 | 浙江省农业科学院 | Accelerate the breeding method of rice milking stage speed |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101663992A (en) * | 2009-06-23 | 2010-03-10 | 江苏沿海地区农业科学研究所 | Cultivation method of high-grain weight excellent-quality ternary hybrid rice variety |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101663992A (en) * | 2009-06-23 | 2010-03-10 | 江苏沿海地区农业科学研究所 | Cultivation method of high-grain weight excellent-quality ternary hybrid rice variety |
Non-Patent Citations (1)
| Title |
|---|
| "优质三系杂交水稻选育的实践与思考";潘润森等;《杂交水稻》;20131231;第28卷(第5期);第19页右栏第1段 * |
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