CN106031369A - Cuora trifasciata hatching method - Google Patents
Cuora trifasciata hatching method Download PDFInfo
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- CN106031369A CN106031369A CN201510123146.XA CN201510123146A CN106031369A CN 106031369 A CN106031369 A CN 106031369A CN 201510123146 A CN201510123146 A CN 201510123146A CN 106031369 A CN106031369 A CN 106031369A
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- sandy soil
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Abstract
The invention relates to a Cuora trifasciata hatching method comprising the following operating steps: preparing river sands and clays in a Cuora trifasciata expected period of confinement, evenly mixing said materials into sandy soil, exposing the sandy soil under the sun, and placing the sandy soil into a standby box body; selecting oosperms and burying the oosperms into the sandy soil at certain intervals without 24 hours after the Cuora trifasciata lays eggs, and transferring the box body into a room; controlling the sand soil water content to be 6-8%, and setting the sandy soil temperature to be 25-27 DEG C so as to hatch male young turtles, or setting the sandy soil to be 29.2-29.8 DEG C so as to hatch female young turtles; regularly checking hatching conditions in every 2-3 days, timely spotting bad eggs and removing the bad eggs. The method can scientifically control the hatching environment temperature and humidity, so the Cuora trifasciata oosperms can be hatched in a better environment, thus effectively improving Cuora trifasciata hatching rate, and scientifically controlling young turtle sex gender proportion; the Cuora trifasciata hatching method can reasonably select sandy soil as hatching medium, is clean in environment, simple in hatching steps, low in cost, high in benefits, and easy to realize.
Description
Technical field
The invention belongs to agricultural and sideline aquaculture technical field, be specifically related to Testudinis cultural technique field, particularly to Cyclemys trifasciata
Hatching method.
Background technology
In recent years, Cyclemys trifasciata artificial cultivation gradually rose, it has also become the cultivation project deeply favored by vast aquaculture family.
Cyclemys trifasciata artificial cultivation still belongs to emerging industry, and small water turtle culture technology is had half knowledge, to the key link in small water turtle culture by most small water turtle culture families--and Cyclemys trifasciata hatching technology more lacks deep understanding.Small water turtle culture family generally uses nature hatching method or carries out Cyclemys trifasciata hatching with reference to other Testudinis kind traditional incubation method at present, not yet grasps the Cyclemys trifasciata hatching technology of science, and therefore Cyclemys trifasciata hatchability is the most on the low side.Additionally, because the sex hatching young Testudinis cannot science be controlled, cause hatching female young Testudinis in young Testudinis on the high side, this male young Testudinis Seedling of Cyclemys trifasciata that small water turtle cultivation farm not only can be caused to need cannot be met, ultimately limit the scale development of small water turtle culture, also can affect the income at small water turtle culture family and the Population breeding of Cyclemys trifasciata.
Small water turtle culture family takes Cyclemys trifasciata Seedling for seeking at present, the modes such as purchase, seizure are generally used to obtain Cyclemys trifasciata Seedling, this not only increases the input cost of raiser, make again Cyclemys trifasciata Seedling size mix, physical condition uneven, be not easy to unified management, batch cultivation.Therefore, in order to promote the sustainable and healthy development of small water turtle culture industry, Cyclemys trifasciata hatching technology is carried out scientific research the most necessary.
Summary of the invention
The technical problem to be solved provides a kind of hatchability and science of being effectively improved to control to hatch the Cyclemys trifasciata hatching method of young Testudinis sex ratio.
For solving above-mentioned technical problem, the technical scheme that the present invention realizes is: a kind of Cyclemys trifasciata hatching method, including following
Operation sequence:
(1) get the raw materials ready: between Cyclemys trifasciata expected date of confinement, get river sand, clay ready and be uniformly mixed into sandy soil, insert uncovered casing or perisporium has in the casing of passage stand-by through sun sterilization of being exposed to the sun;
(2) collect germ cell: Cyclemys trifasciata is laid eggs in latter 24 hours, select germ cell and by it at a certain distance with in degree of depth embedment sandy soil, casing is moved to indoor;Incubation condition: indoor air humidity residing for casing is maintained at 80%~90%, in casing, the water content control of sandy soil is 6%~8%, larger proportion is accounted for if male in the young Testudinis that need to hatch, then sandy soil temperature is controlled at 25 DEG C~27 DEG C, if female Testudinis accounts for larger proportion in the young Testudinis that need to hatch, then sandy soil temperature is controlled at 29.2 DEG C~29.8 DEG C.
(3) periodically check: checked every 2~3 days and once hatch situation, find that bad ovum is removed in time.
In above-mentioned casing, sandy soil thickness is 15~30 centimetres, in described sandy soil, the mixed proportion of river sand and clay is 0.8: 1~1: 0.8, the degree of depth in germ cell embedment sandy soil is 15~30 centimetres, and the spacing between each germ cell is 2~5 centimetres.In described sandy soil, the particle diameter of river sand is 0.3~1.0 millimeter, and the particle diameter of clay is 0.05~0.2 millimeter.
Mixed with the Vermiculitum that particle diameter is 0.3~0.7 millimeter in described sandy soil.
If in time germ cell not being collected in casing in 24 hours after Cyclemys trifasciata is laid eggs, then germ cell is stayed put, and insert board mark in its vicinity.
The beneficial effects of the present invention is
1, the present invention is by controlling the science of environment of hatching temperature and humidity, makes Cyclemys trifasciata germ cell hatch in the environment of preferably, is effectively improved Cyclemys trifasciata hatchability and science controls to hatch the sex ratio of young Testudinis.
2, the present invention is by the sandy soil rationally selected as incubation medium, and environmentally-friendly sanitary, incubation step is simple, low cost and being easily achieved, and can effectively reduce the input of raiser, save aquaculture cost, increase the benefit.
Detailed description of the invention
The invention discloses a kind of Cyclemys trifasciata hatching method, use this hatching method can be effectively improved hatchability, and energy conservative control hatches the sex ratio of young Testudinis, makes the quantitative proportion of female and male young Testudinis rationalize, and this Cyclemys trifasciata hatching method includes following operation sequence.
(1) get the raw materials ready: between Cyclemys trifasciata expected date of confinement, get river sand, clay ready and be uniformly mixed into sandy soil, insert uncovered casing or perisporium has in the casing of passage stand-by through sun sterilization of being exposed to the sun.
(2) collect germ cell: Cyclemys trifasciata is laid eggs in latter 24 hours, select germ cell and by it at a certain distance with in degree of depth embedment sandy soil, casing is moved to indoor.
Incubation condition: indoor air humidity residing for casing is maintained at 80%~90%, in casing, the water content control of sandy soil is 6%~8%, larger proportion is accounted for if male in the young Testudinis that need to hatch, then sandy soil temperature is controlled at 25 DEG C~27 DEG C, if female Testudinis accounts for larger proportion in the young Testudinis that need to hatch, then sandy soil temperature is controlled at 29.2 DEG C~29.8 DEG C.
(3) periodically check: checked every 2~3 days and once hatch situation, find that bad ovum is removed in time.
During practical operation, need to scarifying before Cyclemys trifasciata is laid eggs, the sandy soil that are exposed to the sun on spawning ground, if desired it is carried out disinfection, sand moisture with hands pinch agglomerating, loose one's grip i.e. to dissipate and be advisable.Careful procuratorial work every day Cyclemys trifasciata to be produced and spawning ground is needed between Cyclemys trifasciata expected date of confinement.When finding that vestige of laying eggs then gets casing ready, casing can be bubble chamber, carton or plastic box, casing neglect greatly germ cell quantity depending on, be typically chosen length, width and height and be respectively 40~80 centimetres, 40~80 centimetres, the casing of 40~60 centimetres.Casing for hatching can be uncovered casing, it is also possible to be the perisporium casing that has passage.The sandy soil after the sun is exposed to the sun sterilization of 15~30 cm thick are put in casing, after Cyclemys trifasciata is laid eggs in 24 hours, the method using light-illuminating is told germ cell and is collected, imbeds in sandy soil, when with light-illuminating Testudinis ovum, if there are white annulus or white macula in ovum central authorities, then it it is germ cell.
During hatching, the moist degree of sandy soil and air humidity directly affect the fetal development of germ cell, air humidity is excessive, sand water content is too high, it is easily caused germ cell to suffocate necrosis, air humidity is too small, sand water content is low, then germ cell is easily downright bad because moisture excessive evaporation " dries up ", therefore by the humid control of sandy soil 6%~8%, air humidity is maintained at 80%~90%, most beneficial for embryo's normal development of germ cell in housing environment.Temperature plays an important role in hatching process, and the height of temperature affects fetal development speed, the length of incubation period and the sex of young Testudinis.
Generally, in the range of 22~33 DEG C, the highest fetal development of the temperature time faster, hatching is the shortest, on the contrary then the fetal development time relatively slow, hatching the longest.Therefore, it is male for can making the major part hatched young Testudinis when being controlled by sandy soil temperature in the range of 25 DEG C~27 DEG C, and when being controlled by sandy soil temperature in the range of 29.2 DEG C~29.8 DEG C, it is female for can making the young Testudinis of the major part hatched.
In casing, sandy soil thickness is advisable with 15~30 centimetres, in order to ensure that sandy soil have good breathability and retentiveness concurrently, in sandy soil, the mixed proportion of river sand and clay is 0.8: 1~1: 0.8, this ratio is conducive to making sandy soil keep suitable humidity and air circulation, make germ cell hatch in suitable environment, be effectively improved hatchability.
Germ cell embedment sandy soil are crossed and deeply easily occurs that germ cell is suffocated, imbedding shallow, easily being changed by ambient temperature and damp condition is affected, therefore the degree of depth in germ cell embedment sandy soil is advisable with 8~14 centimetres, and this depth bounds can preferably ensure that germ cell is hatched in the condition of constant temperature and humidity.The spacing of germ cell should be at 2~5 centimetres, and this distance range had both given the incubation space that germ cell is enough, keep each germ cell not interact, ensured again the concordance of incubation condition.In sandy soil, the particle diameter of river sand is 0.3~1.0 millimeter, the particle diameter of clay is 0.05~0.2 millimeter, particle diameter is that the river sand of 0.3~1.0 millimeter can make sandy soil keep good breathability, particle diameter is that the clay of 0.05~0.2 millimeter then makes sandy soil have preferable retentiveness, plays constant temperature and humidity effect.Certainly, it may also be necessary to joining the Vermiculitum having particle diameter to be 0.3~0.7 millimeter in sandy soil, its Main Function is sandy soil are risen insulation effect, the most also suppress the effect of fungus growth, virus killing, ensure that germ cell is the most infected.
Below in conjunction with specific embodiment, the present invention is further described:
Embodiment 1
Between Cyclemys trifasciata expected date of confinement, every day checks that spawning ground is once, after finding that Cyclemys trifasciata is laid eggs, river sand and clay is got ready by the component ratio of 1: 1, and river sand and clay are uniformly mixed into sandy soil, insert after the sun is exposed to the sun sterilization length, width and height be respectively 50 centimetres, 50 centimetres, stand-by in the casing of 40 centimetres, inserting the sandy soil cladding thickness in casing is 30 centimetres.After Cyclemys trifasciata is laid eggs in 24 hours, using light-illuminating mode to tell germ cell, and germ cell collected and imbed in sandy soil, the degree of depth in germ cell embedment sandy soil is equidistantly to discharge with the interval of 2 centimetres between 10 centimetres, and each germ cell.Then casing is moved to indoor, by the water content control of sandy soil be 6%~8%, the air humidity of hatching casing is maintained at 80%~90% sandy soil temperature and controls to be 29.5 DEG C of constant temperature.Checked every 2 days and once hatch situation, find that bad ovum is removed in time.After about 60 days, young Testudinis pips successively, and major part is female young Testudinis, and young Testudinis needs after hatching to take out in time in order to avoid affecting the hatching of remaining germ cell, until all incubating oosperms are complete.
Embodiment 2
Between Cyclemys trifasciata expected date of confinement, every day checks that spawning ground is once, after finding that Cyclemys trifasciata is laid eggs, river sand and clay is got ready by the component ratio of 0.8: 1, and river sand and clay are uniformly mixed into sandy soil, insert after the sun is exposed to the sun sterilization length, width and height be respectively 60 centimetres, 60 centimetres, stand-by in the casing of 50 centimetres, inserting the sandy soil thickness in casing is 30 centimetres.After Cyclemys trifasciata is laid eggs in 24 hours, using light-illuminating mode tell germ cell and imbed in sandy soil, the degree of depth in embedment sandy soil is equidistantly to discharge with the interval of 3 centimetres between 12 centimetres and each germ cell.Then casing is moved to indoor by the water content control of sandy soil be 7%~8%, the air humidity of hatching casing be maintained at 85%~88%, it is 26.5 DEG C of constant temperature that sandy soil temperature controls.Checked every 3 days and once hatch situation, find that bad ovum is removed in time.After about 65 days, young Testudinis pips successively, and major part is male young Testudinis, and young Testudinis needs after hatching to take out in time in order to avoid affecting the continuation hatching of remaining germ cell, until all incubating oosperms are complete.
If Cyclemys trifasciata fails to find in time germ cell in laying eggs latter 24 hours, do not hatch by above way, but stay at original place hatching and insert board mark in its vicinity.
Above-described embodiment is the preferred embodiment of the present invention, and in addition, the present invention can also have other implementations.It is to say, on the premise of without departing from present inventive concept, any obvious replacement also should fall into protection scope of the present invention.
Claims (2)
1. Cyclemys trifasciata hatching method, including following operation sequence:
(1) get the raw materials ready: between Cyclemys trifasciata expected date of confinement, get river sand ready, clay is uniformly mixed into sandy soil, insert uncovered casing or perisporium has in the casing of passage stand-by through sun sterilization of being exposed to the sun;
(2) collect germ cell: Cyclemys trifasciata was laid eggs in 24 hours, select germ cell and by it at a certain distance with in degree of depth embedment sandy soil, casing is moved to indoor;Incubation condition: indoor air humidity residing for casing is maintained at 80%~90%, in casing, the water content control of sandy soil is 6%~8%, larger proportion is accounted for if male in the young Testudinis that need to hatch, then sandy soil temperature is controlled at 25 DEG C~27 DEG C, if female Testudinis accounts for larger proportion in the young Testudinis that need to hatch, then sandy soil temperature is controlled at 29.2 DEG C~29.8 DEG C;
(3) periodically check: checked every 2~3 days and once hatch situation, find that bad ovum is removed in time;In described casing, sandy soil thickness is 15~30 centimetres, and in sandy soil, the mixed proportion of river sand and clay is 1: 1 or 1: 0.8, and the degree of depth in germ cell embedment sandy soil is 15
~30 centimetres, the phase spacing between each germ cell is at 2~5 centimetres.
2. in sandy soil described in claim 1, the particle diameter of river sand is 0.3~1.0 millimeter, and the particle diameter of clay is 0.05~0.2 millimeter;Containing the Vermiculitum that particle diameter is 0.3~0.7 millimeter in described sandy soil;If germ cell is not collected in casing in laying eggs latter 24 hours by Cyclemys trifasciata in time, then stay at original place hatching, and nearby insert board mark.
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CN109548745A (en) * | 2018-12-07 | 2019-04-02 | 广东省生物资源应用研究所 | A kind of method of low temperature induction tortoise ovum diapause |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN109548745A (en) * | 2018-12-07 | 2019-04-02 | 广东省生物资源应用研究所 | A kind of method of low temperature induction tortoise ovum diapause |
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