CN106018757B - A kind of method of inspection of the anti-mite performance of the anti-mite household textiles of probiotics - Google Patents
A kind of method of inspection of the anti-mite performance of the anti-mite household textiles of probiotics Download PDFInfo
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- CN106018757B CN106018757B CN201610593901.5A CN201610593901A CN106018757B CN 106018757 B CN106018757 B CN 106018757B CN 201610593901 A CN201610593901 A CN 201610593901A CN 106018757 B CN106018757 B CN 106018757B
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/36—Textiles
Abstract
The invention discloses a kind of methods of inspection of the anti-mite performance of the anti-mite household textiles of probiotics, include the following steps：Acarid culture；Acarid inhibiting rate（Pupa and adult mite）Experiment；Culture environment；Number of computations；Kill acarid；As a result it calculates.The present invention places the favorite food of dust mite on test specimen and control sample, by culture sample to the smell of dust mite and the effect of the sense of taste, it is made to be reluctant to enter food area, under defined temperature and humidity conditions, with the anti-mite performance of the suppression mite rate investigation test sample after culture 6 weeks and 8 weeks.
Description
Technical field
Present invention relates particularly to a kind of methods of inspection of the anti-mite performance of the anti-mite household textiles of probiotics.
Background technology
Acarid belongs to the small animal of a kind of build of Arthropoda Arachnoidea Acari, and body size generally all exists
0.5 millimeter or so, there is mouthpart in front end, and feeding habits are various, such as dandruff.It is only secondary in the world it has been found that acarid has more than 50000 kinds
In insect.Many mite species have substantial connection with medical research.The healthy relationship of acarid and people are very close, and acarid divides extensively
The dark corner in room, carpet, mattress, pillow, sofa, air-conditioning, summer sleeping mat etc. are distributed in, wherein the distribution of dust mite is most wide, influences
Maximum, corpse, secretion and the excreta of acarid are all anaphylactogens, can make one allergic dermatitis, asthma, bronchus occur
The medium of the transmissions such as the diseases such as inflammation, ephritis, allergic rhinitis, acarid or bacterium, virus, Chlamydia, therefore acarid is tight
It is detrimental to health again.
Harm of the acarid to human body in order to prevent, row expert set about starting with from the most frequent fabric of human contact, taste
Fabric care product is sent out in runin, goes to solve the problems, such as that acarid endangers.There are mainly three types of usual ways：First, directly kill mite
Worm, such as mite killing method, trapping method；Second is that acarid is not allowed to approach, such as deterrent method；Third, acarid is prevented to enter fabric, such as blocked method.
By taking the methods such as solarization, heating, electromagnetic wave, infrared ray that can make fabric drying, the life condition of acarid is destroyed, when its is aqueous
It then can be dead when amount is below 10%.Deoxidation, the oxygen content reduced in air are also a kind of method of mite killing.Chemical mite killing is such as
Use pyrethrum extract isoborneol, dehydroacetic acid, aromatic carbonate, diphenyl ether, piperazine agriculture, Folithion etc., many desinsections
Agent and fungicide are all good acaricides.Trapping method is first to lure acarid and then kill, this that sex inducement, food has been lured to draw
It lures, lay eggs to lure and be lured with pheromones.Deterrent method be using repellent, this be some carry the smell that fears acarid and
The drug DEET and β-naphthols of the substance of taste, commonly referred to as repellent are representative.Blocked method is not allowed using fine and close fabric
Acarid passes through, and also some situations are further to strengthen this barrier effect using repellent.
Invention content
Goal of the invention：The present invention provides a kind of method of inspection of the anti-mite performance of anti-mite household textiles of probiotics.
Technical solution：A kind of method of inspection of the anti-mite performance of anti-mite household textiles of probiotics, includes the following steps：
（1）Acarid culture：Acarid is placed in culture dish, is placed in that temperature is 24 DEG C -26 DEG C and relative humidity is 60%-80%
Environment in, the culture medium of a soup ladle is poured into culture dish, make culture dish half be powdered substance covering, to prevent mite
Worm escapes, and coats insect glue at the upper strata 1/3rd of substance in culture dish, culture dish is put into glass box, then in glass
300 milliliters of common saturated salt solutions are poured into case, are all after two weeks acarid in culture dish；These acarids can both be used for doing reality
It tests, can also be cultivated by being distributed into new culture dish；
（2）Acarid inhibiting rate（Pupa and adult mite）Experiment：Pass through probiotics anti-mite sample cloth and three pieces of correspondences by three pieces
Untreated fabric is respectively placed in 6 metal dish, and one a diameter of 50 millimeters of circular filter paper is placed in sample cloth center respectively,
Bait is placed around filter paper, about 200 adult dust mites are then put into circumference, cover metal disk cover, edge seal；
（3）Culture environment：6 sealing metal disks in above step are placed in the incubator of dark surrounds and are cultivated, case
Interior temperature is：24 DEG C -26 DEG C, relative humidity is：65%—75%；
（4）Number of computations：6 weeks after 8 weeks with taking out test sample, observing under the microscope respectively and calculating weaving sample cloth
The visible mite quantity on upper strata, the work mite of every three parallel samples（Pupa and adult mite）Data, which are added, obtains test sample group work mite
Number compares untreated sample group work mite number；
（5）Kill acarid：Counting finishes, and the sample and vessel there will be dust mite are put into a small amount of clear water, are heated to 100
DEG C continue 10min, dust mite can be killed；
（6）As a result it calculates：Acarid inhibiting rate presses formula（B.1）It calculates：
R=×100……………………………………（B.1）
In formula：
R --- suppression mite rate, unit %；
A --- the untreated control sample group work total sum number of mite, unit is only；
B --- the test sample group work total sum number of mite, unit is only.
As an optimization：The culture dish be in cylindrical type, a diameter of 8 centimetres, a height of 3 centimetres.
As an optimization：The glass box is in cuboid, and length and width are respectively 22 centimetres, a height of 8 centimetres.
Advantageous effect：The present invention places the favorite food of dust mite on test specimen and control sample, is tried by cultivating
Sample makes it be reluctant to enter food area, under defined temperature and humidity conditions, with culture the smell of dust mite and the effect of the sense of taste
Suppression mite rate after 6 weeks and 8 weeks investigates the anti-mite performance of test sample.
Specific embodiment
With reference to specific embodiment, the present invention will be described in detail.
Specific embodiment one：
A kind of method of inspection of the anti-mite performance of anti-mite household textiles of probiotics, includes the following steps：
（1）Acarid culture：Acarid is placed in culture dish, the culture dish be in cylindrical type, a diameter of 8 centimetres, a height of 3 lis
Rice is placed in the environment that temperature is 24 DEG C and relative humidity is 60%, the culture medium of a soup ladle is poured into culture dish, makes culture
The half of ware is covered for powdered substance, and to prevent acarid from escaping, elder brother is coated at the upper strata 1/3rd of substance in culture dish
Culture dish is put into glass box by shellac, and the glass box is in cuboid, and length and width are respectively 22 centimetres, a height of 8 centimetres, then in glass
300 milliliters of common saturated salt solutions are poured into glass case, are all after two weeks acarid in culture dish；These acarids can both be used for doing reality
It tests, can also be cultivated by being distributed into new culture dish；
（2）Acarid inhibiting rate（Pupa and adult mite）Experiment：Pass through probiotics anti-mite sample cloth and three pieces of correspondences by three pieces
Untreated fabric is respectively placed in 6 metal dish, and one a diameter of 50 millimeters of circular filter paper is placed in sample cloth center respectively,
Bait is placed around filter paper, about 200 adult dust mites are then put into circumference, cover metal disk cover, edge seal；
（3）Culture environment：6 sealing metal disks in above step are placed in the incubator of dark surrounds and are cultivated, case
Interior temperature is：24 DEG C, relative humidity is：65%；
（4）Number of computations：6 weeks after 8 weeks with taking out test sample, observing under the microscope respectively and calculating weaving sample cloth
The visible mite quantity on upper strata, the work mite of every three parallel samples（Pupa and adult mite）Data, which are added, obtains test sample group work mite
Number compares untreated sample group work mite number；
（5）Kill acarid：Counting finishes, and the sample and vessel there will be dust mite are put into a small amount of clear water, are heated to 100
DEG C continue 10min, dust mite can be killed；
（6）As a result it calculates：Acarid inhibiting rate presses formula（B.1）It calculates：
R=×100……………………………………（B.1）
In formula：
R --- suppression mite rate, unit %；
A --- the untreated control sample group work total sum number of mite, unit is only；
B --- the test sample group work total sum number of mite, unit is only.
Specific embodiment two：
A kind of method of inspection of the anti-mite performance of anti-mite household textiles of probiotics, includes the following steps：
（1）Acarid culture：Acarid is placed in culture dish, the culture dish be in cylindrical type, a diameter of 8 centimetres, a height of 3 lis
Rice is placed in the environment that temperature is 26 DEG C and relative humidity is 80%, the culture medium of a soup ladle is poured into culture dish, makes culture
The half of ware is covered for powdered substance, and to prevent acarid from escaping, elder brother is coated at the upper strata 1/3rd of substance in culture dish
Culture dish is put into glass box by shellac, and the glass box is in cuboid, and length and width are respectively 22 centimetres, a height of 8 centimetres, then in glass
300 milliliters of common saturated salt solutions are poured into glass case, are all after two weeks acarid in culture dish；These acarids can both be used for doing reality
It tests, can also be cultivated by being distributed into new culture dish；
（2）Acarid inhibiting rate（Pupa and adult mite）Experiment：Pass through probiotics anti-mite sample cloth and three pieces of correspondences by three pieces
Untreated fabric is respectively placed in 6 metal dish, and one a diameter of 50 millimeters of circular filter paper is placed in sample cloth center respectively,
Bait is placed around filter paper, about 200 adult dust mites are then put into circumference, cover metal disk cover, edge seal；
（3）Culture environment：6 sealing metal disks in above step are placed in the incubator of dark surrounds and are cultivated, case
Interior temperature is：26 DEG C, relative humidity is：75%；
（4）Number of computations：6 weeks after 8 weeks with taking out test sample, observing under the microscope respectively and calculating weaving sample cloth
The visible mite quantity on upper strata, the work mite of every three parallel samples（Pupa and adult mite）Data, which are added, obtains test sample group work mite
Number compares untreated sample group work mite number；
（5）Kill acarid：Counting finishes, and the sample and vessel there will be dust mite are put into a small amount of clear water, are heated to 100
DEG C continue 10min, dust mite can be killed；
（6）As a result it calculates：Acarid inhibiting rate presses formula（B.1）It calculates：
R=×100……………………………………（B.1）
In formula：
R --- suppression mite rate, unit %；
A --- the untreated control sample group work total sum number of mite, unit is only；
B --- the test sample group work total sum number of mite, unit is only.
Specific embodiment three：
A kind of method of inspection of the anti-mite performance of anti-mite household textiles of probiotics, includes the following steps：
（1）Acarid culture：Acarid is placed in culture dish, the culture dish be in cylindrical type, a diameter of 8 centimetres, a height of 3 lis
Rice is placed in the environment that temperature is 25 DEG C and relative humidity is 70%, the culture medium of a soup ladle is poured into culture dish, makes culture
The half of ware is covered for powdered substance, and to prevent acarid from escaping, elder brother is coated at the upper strata 1/3rd of substance in culture dish
Culture dish is put into glass box by shellac, and the glass box is in cuboid, and length and width are respectively 22 centimetres, a height of 8 centimetres, then in glass
300 milliliters of common saturated salt solutions are poured into glass case, are all after two weeks acarid in culture dish；These acarids can both be used for doing reality
It tests, can also be cultivated by being distributed into new culture dish；
（2）Acarid inhibiting rate（Pupa and adult mite）Experiment：Pass through probiotics anti-mite sample cloth and three pieces of correspondences by three pieces
Untreated fabric is respectively placed in 6 metal dish, and one a diameter of 50 millimeters of circular filter paper is placed in sample cloth center respectively,
Bait is placed around filter paper, about 200 adult dust mites are then put into circumference, cover metal disk cover, edge seal；
（3）Culture environment：6 sealing metal disks in above step are placed in the incubator of dark surrounds and are cultivated, case
Interior temperature is：25 DEG C, relative humidity is：70%；
（4）Number of computations：6 weeks after 8 weeks with taking out test sample, observing under the microscope respectively and calculating weaving sample cloth
The visible mite quantity on upper strata, the work mite of every three parallel samples（Pupa and adult mite）Data, which are added, obtains test sample group work mite
Number compares untreated sample group work mite number；
（5）Kill acarid：Counting finishes, and the sample and vessel there will be dust mite are put into a small amount of clear water, are heated to 100
DEG C continue 10min, dust mite can be killed；
（6）As a result it calculates：Acarid inhibiting rate presses formula（B.1）It calculates：
R=×100……………………………………（B.1）
In formula：
R --- suppression mite rate, unit %；
A --- the untreated control sample group work total sum number of mite, unit is only；
B --- the test sample group work total sum number of mite, unit is only.
The present invention places the favorite food of dust mite on test specimen and control sample, by culture sample to dust mite
Smell and the sense of taste effect, make its be reluctant enter food area, under defined temperature and humidity conditions, with culture 6 weeks and 8 weeks after
Suppression mite rate investigate the anti-mite performance of test sample.
Dust mite can be harmful to personnel or environment used by this experiment, therefore experiment should be by instructing through correlation detection technology
Experienced and experienced testing staff is engaged in.User has a responsibility for taking appropriate safe and healthy measure and specific seemingly measure, and
Ensure compliance with the national condition in relation to rules and regulations.
The present invention is not limited to above-mentioned preferred forms, anyone can show that other are various under the enlightenment of the present invention
The product of form, however, make any variation in its shape or structure, it is every that there is skill identical or similar to the present application
Art scheme, is within the scope of the present invention.
Claims (1)
1. a kind of method of inspection of the anti-mite performance of anti-mite household textiles of probiotics, it is characterised in that：Include the following steps：
(1) acarid culture：Acarid is placed in culture dish, is placed in that temperature is 24 DEG C -26 DEG C and relative humidity is 60%-80%'s
In environment, the culture medium of a soup ladle is poured into culture dish, the half of culture dish is made to be covered for powdered substance, to prevent acarid
It escapes, coats insect glue at the upper strata 1/3rd of substance in culture dish, culture dish is put into glass box, then in glass box
In pour into 300 milliliters of common saturated salt solutions, be all after two weeks acarid in culture dish；These acarids can both be used for testing,
It can also be cultivated by being distributed into new culture dish；
(2) acarid inhibiting rate is tested：By three pieces by probiotics anti-mite sample cloth and the untreated fabric difference of three pieces of correspondences
It is placed in 6 metal dish, one a diameter of 50 millimeters of circular filter paper is placed in sample cloth center respectively, is placed around filter paper
Then bait is put into about 200 adult dust mites in circumference, covers metal disk cover, edge seal；
(3) culture environment：6 sealing metal disks in above step are placed in the incubator of dark surrounds and are cultivated, it is warm in case
It spends and is：24 DEG C -26 DEG C, relative humidity is：65% -75%；
(4) number of computations：6 weeks after 8 weeks with taking out test sample, observing under the microscope respectively and calculating weaving sample cloth upper strata
Visible mite quantity, work mite (pupa and adult mite) data of every three parallel samples be added obtain test sample group live mite number or
Compare untreated sample group work mite number；
(5) acarid is killed：Counting finishes, and the sample and vessel there will be dust mite are put into a small amount of clear water, is heated to 100 DEG C and holds
Continuous 10min, dust mite can be killed；
(6) result calculates：Acarid inhibiting rate is calculated by formula (B.1)：
In formula：
R --- suppression mite rate, unit %；
A --- the untreated control sample group work total sum number of mite, unit is only；
B --- the test sample group work total sum number of mite, unit is only；
The culture dish be in cylindrical type, a diameter of 8 centimetres, a height of 3 centimetres；The glass box is in cuboid, and length and width are respectively 22 lis
Rice, a height of 8 centimetres.
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| CN201610593901.5A CN106018757B (en) | 2016-07-26 | 2016-07-26 | A kind of method of inspection of the anti-mite performance of the anti-mite household textiles of probiotics |
| PCT/CN2016/105678 WO2018018785A1 (en) | 2016-07-26 | 2016-11-14 | Method for checking anti-mite performance of probiotic anti-mite household textile |
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| CN201610593901.5A CN106018757B (en) | 2016-07-26 | 2016-07-26 | A kind of method of inspection of the anti-mite performance of the anti-mite household textiles of probiotics |
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| CN106018757B (en) * | 2016-07-26 | 2018-07-03 | 紫罗兰家纺科技股份有限公司 | A kind of method of inspection of the anti-mite performance of the anti-mite household textiles of probiotics |
| CN107027712A (en) * | 2017-04-07 | 2017-08-11 | 合肥天敌之家生物技术有限公司 | A kind of colouring method of tyrophagus putrescentiae digestive system |
| CN109187875B (en) * | 2018-09-27 | 2021-05-04 | 广州立白企业集团有限公司 | Anti-mite effect testing method |
| CN110320350B (en) * | 2019-08-09 | 2020-04-21 | 安徽中弘鑫源纺织股份有限公司 | Check out test set of anti-mite household textile anti-mite performance |
| CN112525872A (en) * | 2020-11-09 | 2021-03-19 | 佛山欧神诺陶瓷有限公司 | Ceramic tile anti-mite effect testing method |
| CN112525756A (en) * | 2020-11-11 | 2021-03-19 | 佛山欧神诺陶瓷有限公司 | Method for testing cockroach prevention effect of ceramic tile |
| CN113640503B (en) * | 2021-10-14 | 2021-12-17 | 南通酷依工贸有限公司 | Inspection device for anti-mite performance of anti-mite household textile |
| CN116183467B (en) * | 2023-05-04 | 2023-06-27 | 苏州太湖雪丝绸股份有限公司 | Textile comprehensive detection rating system and rating method thereof |
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| CN1836509A (en) * | 2005-03-25 | 2006-09-27 | 上海杜梆技术有限公司 | Use of N,N-diethylphenylacetamide compound in mite-resistance and bacteria-suppression |
| CN102647907A (en) * | 2009-11-20 | 2012-08-22 | 出光精密科技株式会社 | Composition, antibacterial processing agent and antibacterial molded article |
| CN104988725A (en) * | 2015-06-09 | 2015-10-21 | 广州立白企业集团有限公司 | Mite-resistant fabric care composite |
| CN105155258A (en) * | 2015-07-16 | 2015-12-16 | 广州立白企业集团有限公司 | Anti-mite fabric care composition and application method thereof |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US5275955A (en) * | 1985-07-19 | 1994-01-04 | Gesellschaft Fur Hausbiologische Forschung Mbh | Procedure for establishing the presence of live house-dust mites |
| CN203414462U (en) * | 2013-08-16 | 2014-01-29 | 海宁市伊丽特针织有限公司 | Colored cotton fabric antibacterial detecting device |
| CN103760323A (en) * | 2014-01-09 | 2014-04-30 | 中华人民共和国苏州出入境检验检疫局 | Safety testing method for antibacterial textiles |
| CN106018757B (en) * | 2016-07-26 | 2018-07-03 | 紫罗兰家纺科技股份有限公司 | A kind of method of inspection of the anti-mite performance of the anti-mite household textiles of probiotics |
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- 2016-07-26 CN CN201610593901.5A patent/CN106018757B/en active Active
- 2016-11-14 WO PCT/CN2016/105678 patent/WO2018018785A1/en active Application Filing
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1836509A (en) * | 2005-03-25 | 2006-09-27 | 上海杜梆技术有限公司 | Use of N,N-diethylphenylacetamide compound in mite-resistance and bacteria-suppression |
| CN102647907A (en) * | 2009-11-20 | 2012-08-22 | 出光精密科技株式会社 | Composition, antibacterial processing agent and antibacterial molded article |
| CN104988725A (en) * | 2015-06-09 | 2015-10-21 | 广州立白企业集团有限公司 | Mite-resistant fabric care composite |
| CN105155258A (en) * | 2015-07-16 | 2015-12-16 | 广州立白企业集团有限公司 | Anti-mite fabric care composition and application method thereof |
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| WO2018018785A1 (en) | 2018-02-01 |
| CN106018757A (en) | 2016-10-12 |
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