KR102311287B1 - Composition for control of Dermanyssus gallinae comprising chlorine dioxide and chitin dissolving agent and control method using it - Google Patents

Composition for control of Dermanyssus gallinae comprising chlorine dioxide and chitin dissolving agent and control method using it Download PDF

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KR102311287B1
KR102311287B1 KR1020170170969A KR20170170969A KR102311287B1 KR 102311287 B1 KR102311287 B1 KR 102311287B1 KR 1020170170969 A KR1020170170969 A KR 1020170170969A KR 20170170969 A KR20170170969 A KR 20170170969A KR 102311287 B1 KR102311287 B1 KR 102311287B1
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chlorine dioxide
composition
chicken
chitin
mites
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KR20190070456A (en
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조정혁
김효준
박순심
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주식회사 오투파워
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
    • A01M7/00—Special adaptations or arrangements of liquid-spraying apparatus for purposes covered by this subclass
    • A01M7/0003—Atomisers or mist blowers
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
    • A01N25/02—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests containing liquids as carriers, diluents or solvents
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N29/00—Biocides, pest repellants or attractants, or plant growth regulators containing halogenated hydrocarbons
    • A01N29/02—Acyclic compounds or compounds containing halogen attached to an aliphatic side-chain of a cycloaliphatic ring system
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
    • A01N31/02—Acyclic compounds
    • Y10S514/919—

Abstract

본 발명은 0.005~0.1중량% 농도의 이산화염소용액과; 염화리튬, 트리클로로아세트산, 포름산 및 극성 유기용매를 포함하는 키틴 용해제를 10:1~5의 중량비로 포함하는 닭 진드기 방제용 조성물과 이를 이용한 닭 진드기 방제방법에 관한 것이다. 본 발명은 닭 진드기를 사멸시킬 수 있는 이산화염소와 닭 진드기 성충 외피의 키틴질을 용해시켜 이산화염소의 침투를 용이하게 하는 키틴 용해제를 혼합하여 사용함으로써 닭 진드기의 성충은 물론이고 알과 애벌레까지 동시에 효과적으로 방제할 수 있다. The present invention is a chlorine dioxide solution at a concentration of 0.005 to 0.1% by weight; To a composition for controlling chicken mites comprising a chitin solubilizer containing lithium chloride, trichloroacetic acid, formic acid and a polar organic solvent in a weight ratio of 10:1 to 5, and a method for controlling chicken mites using the same. The present invention uses a mixture of chlorine dioxide, which can kill chicken mites, and a chitin solubilizer that dissolves the chitin of the adult chicken mite outer skin to facilitate the penetration of chlorine dioxide, so that not only the adults of the chicken mites, but also the eggs and larvae are simultaneously effectively can be prevented

Figure 112017124029336-pat00006
Figure 112017124029336-pat00006

Description

이산화염소와 키틴 용해제를 포함하는 닭 진드기 방제용 조성물 및 이를 이용한 방제방법 {Composition for control of Dermanyssus gallinae comprising chlorine dioxide and chitin dissolving agent and control method using it}Composition for control of Dermanyssus gallinae comprising chlorine dioxide and chitin dissolving agent and control method using it

본 발명은 양계에 막대한 피해를 주고 있는 닭 진드기를 방제하기 위한 조성물에 관한 것으로, 보다 구체적으로는 이산화염소와 키틴 용해제를 포함하는 닭 진드기 방제용 조성물 및 이를 이용한 닭 진드기의 방제방법에 관한 것이다 The present invention relates to a composition for controlling chicken mites that cause great damage to poultry, and more particularly, to a composition for controlling chicken mites comprising chlorine dioxide and a chitin solubilizer, and a method for controlling chicken mites using the same

닭 진드기(ワクモ: Dermanyssus gallinae; Red mite, Poultry mite)는 절지동물문 거미강 응애목에 속하고 크기는 1mm 이하인 해충이다. Chicken mite (Wakumo: Dermanyssus gallinae; Red mite, Poultry mite) belongs to the Arthropoda Arachnidae, and is a pest with a size of less than 1 mm.

닭 진드기는 지속적으로 양계 농가들을 괴롭히는 불청객으로 닭 진드기에 의한 양계 농가의 피해는 매우 심각하다. 특히 고온다습한 여름철에는 닭 진드기의 번식이 빨라 양계 농가들에게 더 큰 골칫거리가 된다.Chicken mites are an uninvited guest that constantly harasses poultry farms, and the damage to poultry farms caused by chicken mites is very serious. Especially in the hot and humid summer, chicken mites reproduce quickly, which is a big problem for poultry farmers.

최근 시스템화된 계사 구조의 도입에 따라 닭의 일상관리가 기계화되었고 또한 계사 내부의 온·습도 환경도 연간 일정하게 유지된다. 이러한 계사환경으로 인하여 닭 진드기가 상관없이 증식하거나 약제에 대한 저항성을 나타내는 닭 진드기의 출현으로 피해가 가중되고 있는 실정이다.The daily management of chickens has been mechanized with the recent introduction of a systemized house structure, and the temperature and humidity environment inside the house is also kept constant throughout the year. Due to such a cage environment, the damage is aggravated by the appearance of chicken mites that proliferate regardless of whether chicken mites or show resistance to drugs.

닭 진드기는 닭 1마리당 최고 200㎍을 흡혈하므로, 닭의 빈혈, 폐사, 가려움, 불안, 불면으로 인한 스트레스, 면역 저하, 백신효능의 감소, 질병 전파, 산란율 저하, 난질 저하, 작업 효율 저하 등으로 닭 1마리당 약 1,500원 가량의 손실을 입히는 것으로 추정된다. 유럽의 경우 추정한 피해액의 규모는 연간 1억 3천만유로로 우리나라 돈으로 환산하면 1,820억 수준이다. 나라마다 다르지만 60~85% 정도의 닭이 이미 닭 진드기에 감염되어 있다. Chicken mites suck up to 200 μg of blood per chicken, so it can cause anemia, death, itchiness, anxiety, insomnia, stress, reduced immunity, reduced vaccine efficacy, disease spread, reduced egg production, reduced egg quality, reduced work efficiency, etc. It is estimated that a loss of about 1,500 won per chicken is caused. In the case of Europe, the estimated amount of damage is 130 million euros per year, which is equivalent to 182 billion euros in Korean money. Although it varies from country to country, about 60-85% of chickens are already infected with chicken mites.

닭 진드기의 생활주기를 나타낸 도 1에서와 같이, 닭 진드기는 알에서 2~3일 후 부화하게 된다. 부화 후 유충의 상태로 1~2일이 지나면 제1약충으로 변태를 하고, 제1약충 시기에서 1~2일이 지난 후 제2약충으로 변태를 하며, 다시 2~3일이 지나면 성충으로 변태를 하게 된다. 닭 진드기는 성충이 된 후 12~24시간이 지나면 다시 알을 낳고 번식을 하는 빠른 성장 주기를 가지므로 완벽한 구제가 매우 어려운 실정이다.As shown in FIG. 1 showing the life cycle of chicken mites, chicken mites hatch after 2-3 days from eggs. After hatching, it metamorphoses into the first nymph after 1 to 2 days in the larval state, transforms into the second nymph after 1 to 2 days from the 1st nymph stage, and then transforms into an adult nymph after 2 to 3 days will do Since chicken mites have a rapid growth cycle that lays eggs again and reproduces 12 to 24 hours after they become adults, it is very difficult to get rid of them completely.

현재까지 닭 진드기 방제제로는 신경계 작용물질, 알코올, 탄닌산, 실리카겔 등이 사용되고 있다. Until now, nervous system agents, alcohol, tannic acid, silica gel, etc. have been used as chicken mite control agents.

현재 개발되어 사용 중인 대부분의 약제는 피레스로이드(pyrethroid)계, 카바메이트(carbamate)계, 유기인(organo phosphorus)계 및 페닐피라졸 (phenylpyrezole)계 약제로서 신경계에 작용하는 물질이다. 이들 약제는 주로 중추 신경계나 말단 신경계에 작용하므로 성충이나 제2약충 단계의 진드기에만 그 약효를 나타내어 알이나 유충 상태의 진드기에는 효과가 없다. 따라서, 이러한 약제를 사용하여도 알이나 유충은 살아남게 되므로 곧바로 닭 진드기가 다시 창궐하게 된다.Most of the drugs currently being developed and used are pyrethroid, carbamate, organo phosphorus, and phenylpyrezole drugs that act on the nervous system. Since these drugs mainly act on the central nervous system or the terminal nervous system, they show their efficacy only on adult or second nymph stage mites, and thus have no effect on mites in egg or larval state. Therefore, even if these drugs are used, the eggs and larvae survive, so that the chicken mites re-emerge immediately.

또한 성충이나 제2약충은 외피(exoskeleton)가 키틴(chitin)질로 구성되어 있어 약제가 효과적으로 침투할 수 없는 문제점이 있다. 키틴질은 N-아세틸-D-글루코사민(N-acetyl-D-glucosamine)의 중합체로서, 셀룰로스(cellulose)의 히드록실(hydroxyl)기가 아세틸아미노(acetylamino)기로 모두 치환된 구조를 가짐으로서 인접하는 고분자간의 수소 결합이 가능하여 매우 치밀한 성능을 가지고 있다.In addition, the adult or second nymph has an exoskeleton composed of chitin, so that the drug cannot effectively penetrate. Chitin is a polymer of N-acetyl-D-glucosamine, and has a structure in which all of the hydroxyl groups of cellulose are substituted with acetylamino groups. Hydrogen bonding is possible, so it has very dense performance.

또한 사용되는 약제인 아미드라즈(amitraz), 카바릴(carbaryl), 페르메트린(permethrin) 등에 대한 내성이 증가하고(Zeman & , 1985, Beugnet et al., 1997, Marangi et al.,2009), 닭 진드기의 유전적 변이가 출현한 것(Bret al., 2008, Potenza et al., 2009 ,Roy & Buronfosse, 2011)이 보고됨으로써 닭 진드기 구제제의 시급한 개발이 촉구되고 있다.In addition, resistance to drugs used such as amidraz, carbaryl, permethrin, etc. increased (Zeman & , 1985, Beugnet et al., 1997, Marangi et al., 2009), chicken The emergence of genetic mutations in ticks (Bret al., 2008, Potenza et al., 2009, Roy & Buronfosse, 2011) has been reported, prompting the urgent development of a chicken mite control agent.

닭 진드기 구제에 관한 특허기술로, 대한민국 특허공개 제10-2017-0020768호는 4-[5-(3,5-디클로로페닐)-4,5-디하이드로-5-(트리플루오로메틸)이소옥사졸-3-일]-2-메틸-N-[2-옥소-2-[(2,2,2-트리플루오로에틸)아미노]에틸] 벤즈아미드 또는 (Z)-4-[5-(3,5-디클로로페닐)-5-트리플루오로메틸-4,5-디하이드로이소옥사졸-3-일] -N-(메톡시이미노메틸)-2-메틸안식향산아미드를 주성분으로 하는, 살충, 살진드기, 살선충, 살연체동물, 살균 또는 살박테리아 조성물 및 병해충의 방제방법을 개시하고 있다. 또한 대한민국 특허등록 제10-1295228호는 개미산 및 피리다벤을 주성분으로 하는 축사 및 양계 소독용 살충제 조성물 및 이의 제조방법을 개시하고 있으며, 대한민국 특허등록 제10-1537197호는 비결정형 실리카, 중탄산나트륨(NaHCO3) 또는 이산화규소(SiO2)를 주성분으로 하는 가금류의 와구모 사멸용 살포제 및 가금류의 와구모 사멸방법 및 상기 가금류 와구모 사멸방법으로부터 생산된 가금류 및 알을 개시하고 있다. 또한 대한민국 특허등록 제10-1705543호는 다공성의 산호석(fossilized coral)을 주성분으로 하는 와구모 유인용 조성물 및 그를 포함하는 포충기를 개시하고 있고, 대한민국 특허등록 제10-1505018호는 국화과 식물, 콩과식물, 천남성과 식물, 백부과 식물, 소나무과 식물 및 개구리밥과 식물을 주성분으로 하는 해충제거제의 제조방법 및 이에 의하여 제조된 해충제거제를 개시하고 있다. As a patent technology for chicken mite control, Korean Patent Publication No. 10-2017-0020768 discloses 4-[5-(3,5-dichlorophenyl)-4,5-dihydro-5-(trifluoromethyl)iso Oxazol-3-yl]-2-methyl-N-[2-oxo-2-[(2,2,2-trifluoroethyl)amino]ethyl]benzamide or (Z)-4-[5- (3,5-dichlorophenyl)-5-trifluoromethyl-4,5-dihydroisooxazol-3-yl] -N-(methoxyiminomethyl)-2-methylbenzoic acid amide as a main component, insecticide , a mites, nematodes, molluscs, bactericidal or bactericidal compositions and methods for controlling pests are disclosed. In addition, Korean Patent Registration No. 10-1295228 discloses a pesticide composition for sterilizing livestock and poultry chickens containing formic acid and pyridaben as main components and a method for manufacturing the same, and Korean Patent Registration No. 10-1537197 discloses amorphous silica, sodium bicarbonate ( NaHCO 3 ) or silicon dioxide (SiO 2 ) Disclosed are a pesticide for killing wagumo of poultry and a method for killing wagumo of poultry and poultry and eggs produced from the method for killing wagumo of poultry, mainly composed of silicon dioxide (SiO 2 ). In addition, Korean Patent Registration No. 10-1705543 discloses a composition for attracting Wagumo containing porous coral as a main component and a caterpillar comprising the same, and Korean Patent Registration No. 10-1505018 discloses Asteraceae plants and legumes , Disclosed are a method for producing a pest control agent containing as a main component a plant of the family Apiaceae, a plant belonging to the family Pineaceae, a plant of the family Pineaceae, and a plant of the family Lepidoptera, and the pest control agent manufactured thereby.

또한 서울대의 보고서인 "천연 집 먼지 진드기 중화제 및 닭 진드기 살비제 개발(2009년)"을 포함하여 국내외에서 닭 진드기를 방제하기 위한 다양한 연구가 진행되고 있다. In addition, various studies are underway to control chicken mites at home and abroad, including the Seoul National University report "Development of a natural house dust mite neutralizer and chicken mite acaricide (2009)".

이와 같이, 닭 진드기는 우리나라뿐만 아니라 전세계적으로 문제가 되고 있으며 지금까지 진드기를 방제하기 위한 수많은 노력이 있었지만 아직까지도 진드기를 완벽히 제거하는 것은 불가능에 가깝다.As such, chicken mites are a problem not only in Korea but also worldwide, and there have been numerous efforts to control mites, but it is still close to impossible to completely remove mites.

이산화염소(ClO2)는 대표적인 살균, 탈취제로서(Volk et al. 2002), 염소 등 염소계 살균제와 달리 소독부산물로서 발암성을 가져 유해한 트리할로메탄 (THM, TriHaloMethane)을 생성하지 않고(Don 1998), 물에 대한 용해도가 높고, pH에 무관한 효과를 가지며, 액상 및 기체 상태의 제제화가 가능하여 여러 용도의 소독제로 사용 가능성을 넓히고 있다. 최근 이산화염소는 야채 등의 식품과 과일 등의 장기 보관 및 수출 운반에 널리 사용되고 있다(식품의약품안전청고시 제2007-74호). Chlorine dioxide (ClO 2 ) is a representative sterilizing and deodorizing agent (Volk et al. 2002), and unlike chlorine-based disinfectants such as chlorine, it has carcinogenicity as a disinfection by-product and does not generate harmful trihalomethanes (THM, TriHaloMethane) (Don 1998). ), it has a high solubility in water, has an effect independent of pH, and can be formulated in liquid and gaseous states, thereby expanding the possibility of using it as a disinfectant for various purposes. Recently, chlorine dioxide has been widely used for long-term storage and export transportation of foods such as vegetables and fruits (KFDA Notice No. 2007-74).

이산화염소는 강력한 산화력으로 세균 및 바이러스를 제거하는데, 이는 미생물의 구성 기본 물질인 아미노산 중 시스테인, 트립토판, 세린, 타이로신 등과 같이 OH 나 -SH 작용기를 가지고 있는 아미노산을 강력하게 산화시켜 각각 -C=O 형태의 카르보닐과 -S=O 형태의 설폰(설폭시드)으로 변화시킴으로써 기능이 정지되고 형태가 변형되도록 하는 것으로 알려져 있다. 이산화염소에 의한 아미노산 산화과정을 도 2에 나타내었다. Chlorine dioxide removes bacteria and viruses with its strong oxidizing power, which strongly oxidizes amino acids that have OH or -SH functional groups such as cysteine, tryptophan, serine, and tyrosine among amino acids that are basic substances of microorganisms, and each -C=O It is known that by changing the carbonyl in the form and the sulfone (sulfoxide) in the -S=O form causes the function to stop and the conformation to change. The amino acid oxidation process by chlorine dioxide is shown in FIG. 2 .

이산화염소에 의해 아미노산이 변형됨으로써, 미생물은 단백질 합성을 할 수 없거나 단백질이 생촉매 기능을 상실하게 되므로 사멸된다(Bernarde, M.A., et al., 1967., Kinetics and Mechanism of Bacterial Disinfection by Chlorine Dioxide, J. Appl. Microbiol. 15(2):257.)As amino acids are modified by chlorine dioxide, microorganisms cannot synthesize proteins or proteins lose their biocatalytic function and are thus killed (Bernarde, MA, et al ., 1967., Kinetics and Mechanism of Bacterial Disinfection by Chlorine Dioxide, J. Appl. Microbiol. 15(2):257.)

또한, 이산화염소는 미생물의 세포막 단백질과 지방산 등과 반응(산화)하여 세포막을 파괴하면서(Chen et al. 2002 Wang et al. 2010) K+, Ca2 + 및 Mg2 + 이온 등의 세포 내부물질을 용출되게 하며(Zhang et.al. 2007 Wei et al. 2008), 세포 내 세포핵의 DNA, RNA 등을 산화 변형시키기도 하는 것으로 알려져 있다. 이산화염소에 의한 핵산파괴를 도 3에 나타내었다.Furthermore, chlorine dioxide is the intracellular substance such as destruction of the cell membrane protein and the fatty acid as the reaction cell membrane with (oxidation) of the microorganism (Chen et al. 2002 Wang et al. 2010) K +, Ca 2 + and Mg 2 + ions It is known to elute (Zhang et.al. 2007, Wei et al. 2008), and also to oxidatively modify DNA, RNA, etc. in the nucleus of a cell. The destruction of nucleic acids by chlorine dioxide is shown in FIG. 3 .

최근 이산화염소가 곰팡이류인 사카로마이세스 세레비지애(Saccharomyces cerevisiae)를 비활성화시키는 메카니즘을 조사한 결과에 대한 문헌보고에 의하면, 세포 내의 K+, Ca2 + 및 Mg2 + 이온들이 용출되고, 포도당-6-인산 탈수소효소(glucose-6-phosphate dehydrogenase), 구연산염 합성효소(citrate synthase), 포스포프룩토인산화효소(phosphofructokinase)가 저해되어 비활성화되며, 100ppm 이상의 농도에서는 유전자(genomic) DNA도 손상되는 것으로 확인되었다(Zhu et.al.,2012).According to a literature report on the results of investigating the mechanism by which chlorine dioxide inactivates the fungus Saccharomyces cerevisiae, K + , Ca 2 + and Mg 2 + ions in the cell are eluted, and glucose- 6-phosphate dehydrogenase (glucose-6-phosphate dehydrogenase), citrate synthase (citrate synthase), and phosphofructokinase are inhibited and inactivated (Zhu et. al., 2012).

또 다른 연구에 의하면,이산화염소는 포자의 외막을 손상시켜 포자의 발아(germination)를 효과적으로 저해하고(Young et.al.,2003, Radziminsk et.al.,2002), 포자에서 단백질, DNA, 다당류, K+ 이온, Ca2 + 이온을 밖으로 용출시킬 뿐 아니라, 포자의 지방질을 변질시키고 아데노신 가수분해효소 활성까지 저해하는 것으로 나타났다(Wang et.al.,2010). 이산화염소를 처리한 포자를 전자현미경으로 관찰한 결과를 나타낸 도 4와 5에서와 같이, 세포 표면(막)이 뚜렷하게 손상되었고 세포 성분들의 손상도 명백히 확인되었다.According to another study, chlorine dioxide effectively inhibits germination of spores by damaging the outer membrane of spores (Young et.al., 2003, Radziminsk et.al., 2002), and proteins, DNA, and polysaccharides in spores , K + ions, Ca 2 + ions, as well as eluting to the outside, it was shown to alter the lipids of the spores and inhibit adenosine hydrolase activity (Wang et.al., 2010). As shown in FIGS. 4 and 5 showing the results of observing the chlorine dioxide-treated spores with an electron microscope, the cell surface (membrane) was clearly damaged and damage to the cell components was also clearly confirmed.

이와 같이 이산화염소는 세포 내 이온 용출, 대사 경로 중요 효소 저해 및 세포 구조에 심각한 손상을 주어 세균, 바이러스 뿐 아니라 원생동물, 곰팡이(포자 포함)를 사멸시킨다.As such, chlorine dioxide kills not only bacteria and viruses, but also protozoa and fungi (including spores) by eluting ions into cells, inhibiting important enzymes in metabolic pathways, and severely damaging cellular structures.

최근 기체 상태로 훈증 처리가 가능한 이산화염소는 위생해충 및 저곡해충에 대한 살충력을 탁월하게 나타내고 있다. 병원시설에 발생하는 빈대류(Cimex lectularius, Cimex hemipterus)에 대해서는 비교적 높은 농도(약 1,000ppm)의 이산화염소에 노출시켰을 때 속효성의 방제효과를 나타냈으며(Gibbs et al. 2012), 저곡류를 가해하는 화랑곡나방(Plodia interpunctella)에 대해서는 비교적 낮은 농도(200ppm)의 이산화염소에 노출시켰을 때 완전방제 효과를 나타냈다(Kumar et al. 2015). 또한, 거짓쌀도둑거저리(Tribolium castaneum)의 알, 유충 및 성충의 구제에도 이산화염소는 500ppm이하의 중간 농도에서 탁월한 효능을 나타내었다(Channaiah et.al.).Recently, chlorine dioxide, which can be fumigated in a gaseous state, has excellent insecticidal power against sanitary pests and low-grain pests. For bedbugs (Cimex lectularius, Cimex hemipterus) occurring in hospital facilities, when exposed to a relatively high concentration (about 1,000 ppm) of chlorine dioxide, it showed a fast-acting control effect (Gibbs et al. 2012), and low-grain For Plodia interpunctella, when exposed to a relatively low concentration (200 ppm) of chlorine dioxide, it showed a complete control effect (Kumar et al. 2015). In addition, chlorine dioxide showed excellent efficacy in the control of eggs, larvae and adults of Tribolium castaneum at an intermediate concentration of 500 ppm or less (Channaiah et.al.).

이러한 이산화염소의 살충효과는 이산화염소에 의하여 곤충 체내에서 발생하는 활성산소(Reactive Oxygen Species: ROS)에서 기인하는 것으로 확인되었다(Kumar et al. 2015). ROS는 비교적 다양한 생체분자에 영향을 줄 수 있으며, 생체 내 다양한 기능 단백질의 변형을 유발하여 궁극적으로 살충작용을 나타낸다. This insecticidal effect of chlorine dioxide was confirmed to be due to reactive oxygen species (ROS) generated in the insect body by chlorine dioxide (Kumar et al. 2015). ROS can affect relatively diverse biomolecules, and ultimately exhibit insecticidal action by inducing modification of various functional proteins in vivo.

대한민국 특허공개 제10-2017-0020768호Korean Patent Publication No. 10-2017-0020768 대한민국 특허등록 제10-1295228호Korean Patent Registration No. 10-1295228 대한민국 특허등록 제10-1537197호Korean Patent Registration No. 10-1537197 대한민국 특허등록 제10-1705543호Korean Patent Registration No. 10-1705543 대한민국 특허등록 제10-1505018호Korean Patent Registration No. 10-1505018

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상기 문제점을 해결하기 위하여, 본 발명은 닭 진드기 내부로 침투하여 체내 성분을 산화 및 변형시키고 성충의 호흡기로 침투하여 기도 등을 파괴하며 산화 스트레스를 일으켜 닭 진드기를 사멸하게 하는 이산화염소와, 닭 진드기 성충 외피의 키틴질을 용해시켜 이산화염소의 침투를 용이하게 하는 키틴 용해제를 혼합하여 닭 진드기의 성충은 물론 알과 애벌레를 동시에 구제할 수 있는 닭 진드기 방제용 조성물 및 닭 진드기 방제방법을 제공하는 것을 목적으로 한다. In order to solve the above problems, the present invention is chlorine dioxide that penetrates into the chicken mite, oxidizes and transforms body components, penetrates into the respiratory tract of adults, destroys the airways, and causes oxidative stress to kill the chicken mite, and the chicken mite To provide a chicken mite control composition and a chicken mite control method that can simultaneously control eggs and larvae as well as adults of chicken mites by mixing a chitin solubilizer that dissolves the chitin of the adult shell and facilitates the penetration of chlorine dioxide do it with

상기 목적을 달성하기 위하여, 본 발명은 0.005~0.1중량% 농도의 이산화염소용액과; 염화리튬, 트리클로로아세트산, 포름산 및 극성 유기용매를 포함하는 키틴 용해제를 10:1~5의 중량비로 포함하는 닭 진드기 방제용 조성물을 제공한다.In order to achieve the above object, the present invention is a chlorine dioxide solution with a concentration of 0.005 to 0.1% by weight; It provides a composition for controlling chicken mites comprising a chitin solubilizer containing lithium chloride, trichloroacetic acid, formic acid and a polar organic solvent in a weight ratio of 10:1 to 5.

상기 조성물에서, 상기 이산화염소용액의 용매는 물 또는 유기용매인 것이 바람직하다.In the composition, the solvent of the chlorine dioxide solution is preferably water or an organic solvent.

상기 조성물에서, 상기 유기용매는 디에틸 에테르 등 에테르류, 아세톤 등 케톤류, 사염화탄소 등 할로겐화 탄화수소류, 헥산 등 탄화수소류, 디메틸포름아미드 및 디메틸설폭시드 중 선택된 1종 또는 2종 이상인 것이 바람직하다.In the composition, the organic solvent is preferably one or two or more selected from ethers such as diethyl ether, ketones such as acetone, halogenated hydrocarbons such as carbon tetrachloride, hydrocarbons such as hexane, dimethylformamide and dimethylsulfoxide.

상기 조성물에서, 상기 극성 유기용매는 디메틸포름아미드, 디메틸설폭시드 및 디메틸아세트아미드 중 선택된 1종 또는 2종 이상의 혼합물인 것이 바람직하다.In the composition, the polar organic solvent is preferably one or a mixture of two or more selected from dimethylformamide, dimethylsulfoxide and dimethylacetamide.

상기 조성물에서, 상기 키틴 용해제는 염화리튬, 트리클로로아세트산 및 포름산을 극성 유기용매에 각 성분의 농도가 0.5~10.0중량%가 되도록 용해시킨 용액인 것이 바람직하다.In the composition, the chitin solubilizing agent is preferably a solution in which lithium chloride, trichloroacetic acid and formic acid are dissolved in a polar organic solvent so that the concentration of each component is 0.5 to 10.0% by weight.

상기 조성물에서, 상기 키틴 용해제는 염화리튬, 트리클로로아세트산 및 포름산을, 디메틸포름아미드, 디메틸설폭시드 및 디메틸아세트아미드 중 선택된 1종 또는 2종 이상의 용매에 각 성분의 농도가 0.5~10.0중량%가 되도록 용해시킨 용액인 것이 바람직하다.In the composition, the chitin solubilizing agent is lithium chloride, trichloroacetic acid and formic acid, and the concentration of each component in one or two or more solvents selected from among dimethylformamide, dimethylsulfoxide and dimethylacetamide is 0.5 to 10.0% by weight. It is preferable that it is the solution which melt|dissolved as much as possible.

상기 조성물은 최종 조성물 총중량에 대하여 0.5~1.0중량%의 부식방지제를 더 포함할 수 있다.The composition may further include a corrosion inhibitor in an amount of 0.5 to 1.0% by weight based on the total weight of the final composition.

상기 조성물에서, 상기 부식방지제는 벤조트리아졸, 벤조산 나트륨, 디헥실아민 및 인산계 화합물 중 선택된 1종 또는 2종 이상의 혼합물인 것이 바람직하다.In the composition, the corrosion inhibitor is preferably one or a mixture of two or more selected from benzotriazole, sodium benzoate, dihexylamine, and a phosphoric acid-based compound.

또한 본 발명은 상기 닭 진드기 방제용 조성물을 닭 진드기에 감염된 계사에 분무하는 것을 특징으로 하는 닭 진드기 방제방법을 제공한다.The present invention also provides a method for controlling chicken mites, characterized in that the composition for controlling chicken mites is sprayed on chicken mite-infected cages.

상기 방법에서, 상기 조성물은 2~3일 간격으로 분무하는 것이 바람직하다. In the method, the composition is preferably sprayed at intervals of 2-3 days.

본 발명의 조성물은 조성물 중의 이산화염소 성분이 외피가 없는 닭 진드기 알과 애벌레의 피부로 바로 침투하거나 키틴 용해제에 의하여 외피가 용해된 성충의 피부로 침투하여 막 단백질, 막 탄수화물 및 체내의 성분의 주요 구성 성분 분자들을 산화 및 변형시키고, 기화하여 닭 진드기의 호흡기로 침투하여 닭 진드기의 기도 등을 파괴하며, 닭 진드기 체내에서 활성산소(ROS)의 발생을 대폭 증가시킴으로써 산화 스트레스를 유발시켜 닭 진드기를 사멸시키고, 조성물 중의 염화리튬/트리클로로아세트산/포름산 성분과 유기물 용해력이 우수한 극성유기용매의 상승작용으로 닭 진드기 성충 외피의 키틴질을 용해시켜 이산화염소의 침투를 용이하게 함으로써 닭 진드기의 성충은 물론이고 알과 애벌레까지 동시에 효과적으로 방제할 수 있다. In the composition of the present invention, the chlorine dioxide component in the composition directly penetrates into the skin of chicken mite eggs and larvae without an integument, or penetrates into the skin of an adult in which the integument is dissolved by a chitin solubilizing agent, so that the main component of the membrane protein, membrane carbohydrate and body It oxidizes and transforms constituent molecules, vaporizes it, penetrates into the respiratory tract of the chicken mite, destroys the airway of the chicken mite, and greatly increases the generation of reactive oxygen species (ROS) in the body of the chicken mite, thereby inducing oxidative stress to kill the mite. By synergistic action of lithium chloride / trichloroacetic acid / formic acid component in the composition and a polar organic solvent with excellent organic substance dissolving power, the chitin of the adult chicken mite skin is dissolved to facilitate the penetration of chlorine dioxide, so that not only the adult chicken mites but also the adult It can effectively control both eggs and larvae at the same time.

도 1은 닭 진드기의 생활주기(Life-Cycle)를 나타낸 것이다.
도 2는 이산화염소에 의한 아미노산 산화과정을 나타낸 것이다.
도 3은 이산화염소에 의한 핵산 파괴를 나타낸 것이다.
도 4와 5는 이산화염소에 의하여 세포막 및 내부물질이 파괴되는 것을 확인한 전자현미경 사진이다.
도 6은 이산화염소 농도와 ROS(Reactive Oxygen Species)의 관계를 확인한 그래프이다. 1 shows the life cycle (Life-Cycle) of a chicken mite.
Figure 2 shows the amino acid oxidation process by chlorine dioxide.
Figure 3 shows the destruction of nucleic acids by chlorine dioxide.
4 and 5 are electron micrographs confirming that the cell membrane and internal materials are destroyed by chlorine dioxide.
6 is a graph confirming the relationship between chlorine dioxide concentration and ROS (Reactive Oxygen Species).

본 발명의 닭 진드기 조성물은 0.005~0.1중량% 농도의 이산화염소용액과; 염화리튬, 트리클로로아세트산, 포름산 및 극성 유기용매를 포함하는 키틴 용해제를 포함한다. The chicken mite composition of the present invention is a chlorine dioxide solution at a concentration of 0.005 to 0.1% by weight; and a chitin solubilizer including lithium chloride, trichloroacetic acid, formic acid and a polar organic solvent.

이하 본 발명을 보다 구체적으로 설명한다.Hereinafter, the present invention will be described in more detail.

(1) 이산화염소용액(1) chlorine dioxide solution

기체 상태의 이산화염소는 아염소산나트륨과 염산의 반응에 의한 제조방법 등 공지의 방법으로 제조하거나, 상업용으로 판매되는 제품을 사용할 수 있다. 예를 들어, (주)데오테크 등이 살균 탈취용으로 공급하는 이산화염소 가스 발생기 오도킬러 제품 등을 사용할 수 있다.Gas-phase chlorine dioxide may be prepared by a known method such as a production method by a reaction of sodium chlorite and hydrochloric acid, or commercially available products may be used. For example, it is possible to use chlorine dioxide gas generator Odokiller products supplied by Deotech, etc. for sterilization and deodorization.

이산화염소는 기체 상태의 이산화염소를 용매에 용해시킨 용액의 형태로 사용한다. Chlorine dioxide is used in the form of a solution of gaseous chlorine dioxide dissolved in a solvent.

이때 용매로는 물이나 유기용매를 사용하며, 유기용매로는 디에틸 에테르 등 에테르류, 아세톤 등 케톤류, 사염화탄소 등 할로겐화 탄화수소류, 헥산 등 탄화수소류, 디메틸포름아미드 및 디메틸설폭시드 중 선택된 1종 또는 2종 이상을 혼합하여 사용한다.At this time, water or an organic solvent is used as the solvent, and as the organic solvent, one selected from ethers such as diethyl ether, ketones such as acetone, halogenated hydrocarbons such as carbon tetrachloride, hydrocarbons such as hexane, dimethylformamide and dimethylsulfoxide, or Two or more types are mixed and used.

이산화염소는 상기 용매에 0.005~0.1중량%의 농도로 용해시켜 사용하며, 0.01~0.05중량%의 농도로 용해시켜 사용하는 것이 보다 바람직하다.Chlorine dioxide is used by dissolving in the solvent at a concentration of 0.005 to 0.1% by weight, more preferably used by dissolving in a concentration of 0.01 to 0.05% by weight.

시판되는 이산화염소 수용액, 예를 들어, (주)케모피아 등이 살균 탈취용으로 공급하는 이산화염소 가스 수용액 제품 등을 사용할 수도 있다.A commercially available chlorine dioxide aqueous solution, for example, a chlorine dioxide gas aqueous solution product supplied by Chemopia Co., Ltd. for sterilization and deodorization, etc. may be used.

이산화염소는 기화되어 닭 진드기 성충의 호흡기로 들어가 호흡기를 파괴하고, 외피가 없는 닭 진드기의 알이나 애벌레의 피부로 바로 침투하거나 키틴 용해제에 의하여 외피가 용해된 성충의 피부로 침투하여 막 단백질, 막 탄수화물 및 체내의 성분의 주요 구성 성분 분자들을 산화 및 변형시켜 닭 진드기를 사멸시킨다. Chlorine dioxide is vaporized and enters the respiratory tract of adult chicken mites and destroys the respiratory tract, and directly penetrates into the skin of chicken mite eggs or larvae without an integument, or penetrates into the skin of an adult whose integument is dissolved by a chitin solubilizer to form membrane proteins and membranes. It kills chicken mites by oxidizing and modifying carbohydrates and major constituent molecules of the body's components.

또한 호흡기, 피부 및 외피를 통해 들어간 이산화염소는 닭 진드기 체내에서 활성산소(ROS)의 발생을 대폭 증가시킴으로써 산화 스트레스를 유발시켜 사멸에 이르게 한다. In addition, chlorine dioxide entering through the respiratory tract, skin and integument significantly increases the generation of reactive oxygen species (ROS) in the body of chicken mites, causing oxidative stress and leading to death.

(2) 키틴 용해제(2) Chitin solubilizer

키틴 용해제는 염화리튬(LiCl, lithium chloride), 트리클로로아세트산(trichloroacetic acid), 포름산(formic acid) 및 극성 유기용매를 포함한다.The chitin solubilizer includes lithium chloride (LiCl), trichloroacetic acid, formic acid, and a polar organic solvent.

키틴 용해제는 염화리튬, 트리클로로아세트산 및 포름산을 극성 유기용매에 용해시킨 용액의 형태로 사용한다. The chitin solubilizer is used in the form of a solution in which lithium chloride, trichloroacetic acid and formic acid are dissolved in a polar organic solvent.

극성 유기용매로는 디메틸포름아미드(Dimethylformamide, DMF), 디메틸설폭시드(Dimethylsulfoxide, DMSO) 및 디메틸아세트아미드(Dimethylacetamide, DMA) 중 선택된 1종 또는 2종 이상의 혼합물을 사용하는 것이 바람직하다. 극성 유기용매는 유기물을 용해시키는 특성이 우수하여 키틴질 용해력을 강화하는 역할을 한다.As the polar organic solvent, it is preferable to use one or a mixture of two or more selected from among dimethylformamide (Dimethylformamide, DMF), dimethylsulfoxide (DMSO) and dimethylacetamide (DMA). The polar organic solvent has excellent dissolving properties of organic matter, and thus serves to strengthen the chitin dissolving power.

상기 염화리튬, 트리클로로아세트산 및 포름산은 극성 유기용매 중에 각 성분의 농도가 0.5~10.0중량%가 되도록 용해시키는 것이 바람직하며, 각 성분의 농도가 1.0~5.0중량%가 되도록 용해시키는 것이 보다 바람직하다. The lithium chloride, trichloroacetic acid and formic acid are preferably dissolved in a polar organic solvent so that the concentration of each component is 0.5 to 10.0 wt%, and more preferably dissolved so that the concentration of each component is 1.0 to 5.0 wt% .

키틴 용해제는 염화리튬/트리클로로아세트산/포름산 성분과 유기물에 대한 용해력이 우수한 극성 유기용매의 상승작용으로 닭 진드기 성충 외피의 키틴질을 용해시켜 이산화염소의 침투를 용이하게 한다. The chitin solubilizer dissolves the chitin of the adult chicken mite skin by synergistic action of lithium chloride/trichloroacetic acid/formic acid components and a polar organic solvent with excellent solubility for organic matter, thereby facilitating the penetration of chlorine dioxide.

(3) 닭 진드기 방제용 조성물(3) Composition for controlling chicken mites

상기 이산화염소와 키틴 용해제를 10:1~5의 중량비로 혼합하여 닭 진드기 방제용 조성물을 제조한다. 이산화염소용액과 키틴용해제는 10:2~3의 중량비로 혼합하는 것이 보다 바람직하다.The chlorine dioxide and chitin solubilizer are mixed in a weight ratio of 10:1 to 5 to prepare a composition for controlling chicken mites. It is more preferable to mix the chlorine dioxide solution and the chitin solubilizer in a weight ratio of 10:2 to 3.

상기 조성물은 이산화염소 성분으로 인하여 별도의 안정제가 필요하지 않지만, 반복되는 분무로 분무기나 계사 구조물이 부식되는 것으로 방지하기 위하여 부식방지제를 더 포함할 수 있다. 부식방지제로는 벤조트리아졸, 벤조산 나트륨, 디헥실아민, 인산계 화합물 등의 통상적으로 사용되는 부식방지제를 사용한다. 부식방지제는 최종 조성물에 0.5~1.0중량% 농도로 첨가하는 것이 바람직하다. The composition does not require a separate stabilizer due to the chlorine dioxide component, but may further include a corrosion inhibitor to prevent the sprayer or cage structure from being corroded by repeated spraying. As the corrosion inhibitor, commonly used corrosion inhibitors such as benzotriazole, sodium benzoate, dihexylamine, and phosphoric acid-based compounds are used. The corrosion inhibitor is preferably added to the final composition in a concentration of 0.5 to 1.0% by weight.

상기 조성물은 분무기를 사용하여 닭 진드기에 감염된 계사에 분무하여 사용한다. 닭 진드기의 성장 변이 단계가 2~3일이므로, 2~3일 간격으로 분무하는 것이 바람직하다. The composition is used by spraying the chicken mite-infected cages using a sprayer. Since the growth mutation stage of chicken mites is 2-3 days, it is preferable to spray every 2-3 days.

이하 실시예를 통하여 본 발명을 보다 구체적으로 설명한다. 이들 실시예는 본 발명을 예시하는 것으로서 본 발명의 범위가 이들에 한정되는 것은 아니다. The present invention will be described in more detail with reference to the following examples. These examples illustrate the present invention, and the scope of the present invention is not limited thereto.

<실시예 1><Example 1>

조성물의 제조Preparation of the composition

25% 아염소산 나트륨 14.4g과 10% 차아염소산 나트륨 14.8g을 물 968.8g과 자석 교반기를 사용하여 혼합하였다. 여기에 황산(98%) 2g을 천천히 적가하면, 0.2 %의 이산화염소 수용액 1Kg 이 생성된다. 생성되는 0.2중량% 농도(농도는 요드화칼륨과 인산 완충액을 사용하여 pH 7 에서 티오황산 나트륨 적정법으로 측정한다)의 이산화염소 수용액을 물로 희석하여 0.01중량%, 0.02중량%, 0.03중량%, 0.04중량% 및 0.05중량% 농도의 이산화염소용액을 제조하였다. 14.4 g of 25% sodium chlorite and 14.8 g of 10% sodium hypochlorite were mixed with 968.8 g of water using a magnetic stirrer. When 2 g of sulfuric acid (98%) is slowly added dropwise thereto, 1 kg of 0.2% chlorine dioxide aqueous solution is produced. The resulting 0.2 wt% concentration (concentration is measured by sodium thiosulfate titration at pH 7 using potassium iodide and phosphate buffer) is diluted with water to 0.01 wt%, 0.02 wt%, 0.03 wt%, 0.04 Chlorine dioxide solutions were prepared at concentrations of wt% and 0.05 wt%.

염화리튬 20g,트리클로로아세트산 50g, 포름산 30g을 디메틸포름아미드 3kg 에 용해시켜 키틴 용해제를 제조하였다. A chitin solubilizer was prepared by dissolving 20 g of lithium chloride, 50 g of trichloroacetic acid, and 30 g of formic acid in 3 kg of dimethylformamide.

상기 제조된 이산화염소용액과 키틴 용해제를 10:3의 중량비로 혼합하고, 부식방지제인 디헥실 아민을 최종 조성물 중량에 대하여 0.5중량% 농도로 첨가하여 조성물을 완성하였다. The prepared chlorine dioxide solution and chitin solubilizer were mixed in a weight ratio of 10:3, and dihexylamine, a corrosion inhibitor, was added at a concentration of 0.5% by weight based on the weight of the final composition to complete the composition.

[[ 실험예Experimental example ]]

<실험장소 및 조건><Experiment location and conditions>

닭 진드기 방제효과에 대한 실험은 경북 봉화군 봉화읍 양계 단지길 91 소재 이레팜에서 2017년 10월에 실시하였다. 실험실 환경은 온도 23±1℃와 상대습도 54±5%를 유지하였다.The experiment on the chicken mite control effect was conducted in October 2017 at Irefarm located at 91 Yanggye Danji-gil, Bonghwa-eup, Bonghwa-gun, Gyeongsangbuk-do. The laboratory environment was maintained at a temperature of 23±1°C and a relative humidity of 54±5%.

<< 실험예Experimental example 1> 1>

닭 진드기 성충에 대한 방제효과 확인Confirmation of control effect on adult chicken mites

본 발명의 조성물의 닭 진드기 성충에 대한 방제효과를 확인하기 위하여 다음과 같이 실험하였다. In order to confirm the control effect of the composition of the present invention on adult chicken mites, an experiment was conducted as follows.

(1) 닭 진드기 채취 및 보관(1) Collection and storage of chicken mites

감염된 산란계에서 진드기를 채취하여, 즉시 직경 10cm, 높이 20cm의 원통에 보관하였다. 원통의 상부에는 20mesh의 망사를 배치하였고, 원통의 바닥에는 여과지(Whatman No.2)를 2장 배치하였으며, 그 위에 직경 2cm의 증류수에 적신 면솜을 10개 배치하였다. 채취한 닭 진드기는 채취 2일 이내에 시험에 사용하였다. Ticks were collected from infected laying hens and immediately stored in a cylinder with a diameter of 10 cm and a height of 20 cm. A 20-mesh mesh was placed on the upper part of the cylinder, two pieces of filter paper (Whatman No. 2) were placed on the bottom of the cylinder, and 10 cotton swabs soaked in distilled water with a diameter of 2 cm were placed on it. The collected chicken mites were used for testing within 2 days of collection.

(2) 실험방법 및 결과(2) Experimental methods and results

1ℓ 비이커 용기에 바닥에는 여과지(Whatman No.2)를 1장 구비하고, 보관된 닭 진드기 성충 30마리를 각각 배치하였다. One piece of filter paper (Whatman No. 2) was provided at the bottom of a 1L beaker container, and 30 stored chicken mite adults were placed respectively.

여기에 상기 실시예 1에서 제조된 각 농도의 조성물 5±1㎖를 마이크로 분무기를 사용하여 분무하였다.Here, 5±1 ml of each concentration of the composition prepared in Example 1 was sprayed using a micro atomizer.

분무 1시간, 3시간, 6시간, 12시간, 24시간 및 36시간 후에 닭 진드기 성충을 가는 주사 바늘로 접촉하여 움직임을 확인하는 방법으로 사멸을 판정하였다. 이산화 염소 농도와 처리후 경과시간에 따른 닭 진드기 성충의 사멸율 변화를 확인한 결과를 하기 표 1에 나타내었다(단위: %).After 1 hour, 3 hours, 6 hours, 12 hours, 24 hours and 36 hours after spraying, the death was determined by contacting the adult chicken mite with a fine needle and checking the movement. The results of confirming the change in the mortality rate of adult chicken mites according to the chlorine dioxide concentration and the elapsed time after treatment are shown in Table 1 below (unit: %).

이산화염소 농도(%)Chlorine dioxide concentration (%) 처리 후
1시간after processing
1 hours 처리 후
3시간after processing
3 hours 처리 후
6시간after processing
6 hours 처리 후
12시간after processing
12 hours 처리 후
24시간after processing
24 hours 처리 후
36시간after processing
36 hours 0.010.01 7575 8989 9999 100100 -- -- 0.020.02 8787 9999 100100 -- -- -- 0.030.03 9696 100100 -- -- -- -- 0.040.04 100100 -- -- -- -- -- 0.050.05 100100 -- -- -- -- --

상기 표 1의 결과에서, 이산화염소의 농도가 높아질수록 처리 후 닭 진드기 성충이 100% 사멸하는 시간이 짧아지는 것을 알 수 있다. 즉, 이산화염소 농도가 0.04중량% 이상에서는 처리 1시간 후에 닭 진드기 성충이 100% 사멸하였고, 0.01중량%의 농도에서도 처리 12시간 후에는 닭 진드기 성충이 100% 사멸하였다. From the results of Table 1, it can be seen that the higher the concentration of chlorine dioxide, the shorter the time for 100% death of adult chicken mites after treatment. That is, at a chlorine dioxide concentration of 0.04% by weight or more, 100% of adult chicken mites died after 1 hour of treatment, and 100% of adult chicken mites died after 12 hours of treatment even at a concentration of 0.01% by weight.

<< 실험예Experimental example 2> 2>

닭 진드기 알에 대한 방제효과 확인Confirmation of control effect on chicken mite eggs

본 발명의 조성물의 닭 진드기 알에 대한 방제효과를 시험하기 위하여 다음과 같이 실험하였다.In order to test the control effect of the composition of the present invention on chicken mite eggs, the experiment was conducted as follows.

(1) 닭 진드기 알의 채취(1) Collection of chicken mite eggs

닭 진드기에 감염된 산란계에서 닭 진드기의 알을 채취하여 즉시 실험에 사용하였다. 채취된 알은 애벌레로의 변이하기 전인 산란 후 2일 이내의 알을 사용하였다.Chicken mite eggs were collected from laying hens infected with chicken mites and immediately used for experiments. The collected eggs were used within 2 days after spawning, which is before transformation into larvae.

(2) 실험방법 및 결과(2) Experimental methods and results

1ℓ 비이커 등 용기에 바닥에는 여과지(Whatman No.2) 1장을 구비하고 닭 진드기 알 10개를 각각 배치하였다. In a container such as a 1L beaker, one filter paper (Whatman No.2) was provided at the bottom, and 10 chicken mite eggs were placed respectively.

여기에 상기 실시예 1에서 제조된 각 농도의 조성물 5±1㎖를 마이크로 분무기를 사용하여 분무하였다.Here, 5±1 ml of each concentration of the composition prepared in Example 1 was sprayed using a micro atomizer.

이산화염소 각 농도당 처리 36시간 후에 알의 발아(germination)을 확인하는 방법으로 알의 사멸을 판정하였다. 그 결과를 하기 표 2에 나타내었다(단위: %).Egg death was determined by checking the germination of eggs after 36 hours of treatment for each concentration of chlorine dioxide. The results are shown in Table 2 below (unit: %).

이산화염소 농도(%)Chlorine dioxide concentration (%) 처리 후 36시간36 hours after treatment 0.010.01 9090 0.020.02 100100 0.030.03 100100 0.040.04 100100 0.050.05 100100

상기 표 2의 결과에서와 같이, 이산화염소 농도 0.02중량% 이상에서는 처리 36시간 후에 닭 진드기 알이 모두 사멸하였다.As shown in the results of Table 2, all chicken mite eggs were killed after 36 hours of treatment at a chlorine dioxide concentration of 0.02 wt% or more.

<< 실험예Experimental example 3> 3>

닭 진드기 애벌레에 대한 방제효과 확인Confirmation of control effect on chicken mite larvae

본 발명의 조성물의 닭 진드기 애벌레에 대한 구제효능을 시험하기 위하여 다음과 같이 실험하였다.In order to test the extermination effect of the composition of the present invention against chicken mite larvae, it was tested as follows.

(1) 닭 진드기 애벌레의 채취(1) Collection of chicken mite larvae

닭 진드기에 감염된 산란계에서 닭 진드기의 애벌레를 채취하여 즉시 실험에 사용하였다. 애벌레는 제1약충으로 변이하기 전인 애벌레 변이 후 1일 이내의 애벌레를 사용하였다.Chicken mite larvae were collected from laying hens infected with chicken mites and immediately used for experiments. The larvae were used within 1 day after the larval transformation, before transformation into the first nymph.

(2) 실험방법 및 결과(2) Experimental methods and results

1ℓ 비이커 등 용기에 바닥에는 여과지(Whatman No.2) 1장을 구비하고 닭 진드기 애벌레 10마리를 각각 배치하였다. One piece of filter paper (Whatman No. 2) was provided on the bottom of a container such as a 1 liter beaker, and 10 chicken mite larvae were placed respectively.

여기에 상기 실시예 1에서 제조된 각 농도의 조성물 5±1㎖를 마이크로 분무기를 사용하여 분무하였다.Here, 5±1 ml of each concentration of the composition prepared in Example 1 was sprayed using a micro atomizer.

이산화염소 각 농도당 처리 36시간 후에 닭 진드기 애벌레의 사멸을 확인하여 결과를 하기 표 3에 나타내었다(단위: %).The death of chicken mite larvae was confirmed after 36 hours of treatment for each concentration of chlorine dioxide, and the results are shown in Table 3 below (unit: %).

이산화염소 농도(%)Chlorine dioxide concentration (%) 처리 후 36시간36 hours after treatment 0.010.01 100100 0.020.02 100100 0.030.03 100100 0.040.04 100100 0.050.05 100100

상기 표 3의 결과에서와 같이, 닭 진드기 애벌레는 모든 이산화염소 농도에서 처리 36시간 후 모두 사멸하였다.As shown in the results of Table 3, chicken mite larvae were all killed after 36 hours of treatment at all chlorine dioxide concentrations.

Claims (10)

0.01~0.05중량% 농도의 이산화염소용액과 키틴 용해제를 10:1~5의 중량비로 포함하며,
상기 키틴 용해제는 염화리튬, 트리클로로아세트산 및 포름산을 극성 유기용매에 각 성분의 농도가 0.5~10.0중량%가 되도록 용해시킨 용액이고,
상기 극성 유기용매는 디메틸포름아미드, 디메틸설폭시드 및 디메틸아세트아미드 중 선택된 1종 또는 2종 이상의 혼합물인 닭 진드기 방제용 조성물.It contains a chlorine dioxide solution with a concentration of 0.01 to 0.05% by weight and a chitin solubilizer in a weight ratio of 10:1 to 5,
The chitin solubilizer is a solution in which lithium chloride, trichloroacetic acid and formic acid are dissolved in a polar organic solvent so that the concentration of each component is 0.5 to 10.0 wt%,
The polar organic solvent is one or a mixture of two or more selected from dimethylformamide, dimethyl sulfoxide and dimethylacetamide, a composition for controlling chicken mites. 제1항에 있어서,
상기 이산화염소용액의 용매는 물 또는 유기용매인 것을 특징으로 하는 닭 진드기 방제용 조성물.According to claim 1,
The solvent of the chlorine dioxide solution is a chicken mite control composition, characterized in that water or an organic solvent. 제2항에 있어서,
상기 유기용매는 디에틸 에테르 등 에테르류, 아세톤 등 케톤류, 사염화탄소 등 할로겐화 탄화수소류, 헥산 등 탄화수소류, 디메틸포름아미드 및 디메틸설폭시드 중 선택된 1종 또는 2종 이상인 것을 특징으로 하는 닭 진드기 방제용 조성물.3. The method of claim 2,
The organic solvent is one or two or more selected from ethers such as diethyl ether, ketones such as acetone, halogenated hydrocarbons such as carbon tetrachloride, hydrocarbons such as hexane, dimethylformamide and dimethylsulfoxide. Composition for controlling chicken mites . 삭제delete 삭제delete 삭제delete 제1항에 있어서,
상기 조성물은 최종 조성물 총중량에 대하여 0.5~1.0중량%의 부식방지제를 더 포함하는 것을 특징으로 하는 닭 진드기 방제용 조성물. According to claim 1,
The composition is a chicken mite control composition, characterized in that it further comprises 0.5 to 1.0% by weight of a corrosion inhibitor based on the total weight of the final composition. 제7항에 있어서,
상기 부식방지제는 벤조트리아졸, 벤조산 나트륨, 디헥실아민 및 인산계 화합물 중 선택된 1종 또는 2종 이상의 혼합물인 것을 특징으로 하는 닭 진드기 방제용 조성물. 8. The method of claim 7,
The corrosion inhibitor is benzotriazole, sodium benzoate, dihexylamine and a composition for controlling chicken mites, characterized in that a mixture of one or two or more selected from a phosphate-based compound. 제1항, 제2항, 제3항, 제7항 및 제8항 중 어느 한 항의 조성물을 닭 진드기에 감염된 계사에 분무하는 것을 특징으로 하는 닭 진드기 방제방법.9. A method for controlling chicken mites, characterized in that the composition of any one of claims 1, 2, 3, 7 and 8 is sprayed on chicken mite-infected hens. 제9항에 있어서,
상기 조성물은 2~3일 간격으로 분무하는 것을 특징으로 하는 방법.10. The method of claim 9,
Method, characterized in that the composition is sprayed at intervals of 2-3 days.
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C.K.S. Pillai 외 2인, "Chitin and chitosan polymers: Chemistry, solubility and fiber formation", Progress in Polymer Science, 34, pp.641-678 (2009.04.11.)*

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