CN105995192B - A kind of preparation method and application of feed of grass carp nano controlled-release sodium butyrate - Google Patents
A kind of preparation method and application of feed of grass carp nano controlled-release sodium butyrate Download PDFInfo
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- CN105995192B CN105995192B CN201610327599.9A CN201610327599A CN105995192B CN 105995192 B CN105995192 B CN 105995192B CN 201610327599 A CN201610327599 A CN 201610327599A CN 105995192 B CN105995192 B CN 105995192B
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Abstract
The invention discloses a kind of feed of grass carp preparation methods of nano controlled-release sodium butyrate, it is related to fish feed technical field.In sodium hydroxide solution, drop evenly n-butyric acie, it is reacted 1.5-2.0 hours after being added dropwise to complete, butyric acid sodium solution, be added auxiliary material in butyric acid sodium solution, the mass ratio of auxiliary material and butyric acid sodium solution is 1-1.5:2, auxiliary material are as follows: 2-3 parts of beeswax, 1-2 parts of palm wax, fish oil 0.1-0.5,1-4 parts of polyethylene glycol, 0.5-1 parts of filler, 1-1.5 parts of nano controlled-release carrier finally carry out being spray-dried up to nano controlled-release sodium butyrate, and drying temperature is 200-220 DEG C.Nanometer embedding is carried out to sodium butyrate using nanometer embedding techniques, overcoming sodium butyrate has peculiar smell, easily deliquesces, it is difficult to be utilized by enteron aisle, the shortcomings that enteron aisle provides energy cannot be continuously.The health for promoting grass carp intestinal improves the absorption of nutriment, improves the economic benefit of grass carp cultivation.
Description
Technical field:
The present invention relates to a kind of feed of grass carp preparation method and applications of nano controlled-release sodium butyrate, belong to fish feed skill
Art field.
Background technique:
Sodium butyrate can improve small broiler chick, promote intestinal cell proliferation and maturation, maintain the normal of Intestinal epithelial cells
Form promotes the digestion and absorption of small intestine, accelerates the speed of growth of grass carp, improves grass carp production performance.
Sodium butyrate is short chain volatile fatty acid butyric acid, is the energy of enterocyte preference.Common sodium butyrate is in crop or stomach
Middle major part is absorbed, and only small part reaches enteron aisle, while gastric acid environment also has large effect to sodium butyrate, therefore common
Sodium butyrate is unable to satisfy each segmentation of enteron aisle to the nutritional need of sodium butyrate.In addition, avoiding feeding process in feed addition
Middle special odor is difficult to reach enteron aisle or the disadvantages of enteron aisle cannot persistently play a role, generallys use film-coating technique processing fourth
It is prepared into coated product by sour sodium.
Sodium butyrate film-coating technique, which mainly passes through, at present selects different types of coated fertilizer to carry out coating to sodium butyrate, to the greatest extent may be used
Can ground protect butyrate not to be absorbed under one's belt, but current technology is also immature, and the dissolution releasing effect in intestinal juice is also not very
Ideal, and do not have duration.
Summary of the invention:
In view of the above-mentioned problems, the technical problem to be solved in the present invention is to provide a kind of feed of grass carp nano controlled-release sodium butyrates
Preparation method, nanometer embedding is carried out to sodium butyrate, improves its stability in grass carp intestinal, after reaching small intestine, covers intestines
Road, slow release have persistence, effectively improve absorption of the grass carp intestinal to nutriment, promote growth, improve productivity
Energy.
Feed of grass carp of the invention nano controlled-release sodium butyrate the preparation method comprises the following steps: step 1: in sodium hydroxide solution,
N-butyric acie is dropped evenly, is reacted 1.5-2.0 hours after being added dropwise to complete, obtains butyric acid sodium solution, wherein sodium hydroxide and n-butyric acie
Mass ratio is 1:1-1.5；
Step 2: being added auxiliary material in the butyric acid sodium solution of step 1, and the mass ratio of auxiliary material and sodium butyrate is 1-1.5:2,
And the auxiliary material are as follows: 2-3 parts of beeswax, 1-2 parts of palm wax, fish oil 0.1-0.5,1-4 parts of polyethylene glycol, 0.5-1 parts of filler,
1-1.5 parts of nano controlled-release carrier, the nano controlled-release carrier is dried the preparation method comprises the following steps: 2.0054g is added in polymerization pipe
Macrogol 4000,3.00mL polycaprolactone and 0.2mL stannous octoate, liquid nitrogen is sudden cold, vacuumizes, and logical nitrogen drains oxygen in pipe
Gas is stirred to react 4h for 135 DEG C after tube sealing, product is transferred in small beaker, and ether precipitating is added, filters, filter residue dichloromethane
Alkane dissolution, is added methanol extraction, filters, dry 0.5h in vacuum drying oven；
Step 3: the mix products of butyric acid sodium solution and auxiliary material that step 2 obtains are spray-dried and are delayed up to nanometer
Sodium butyrate is released, drying temperature is 200-220 DEG C.
The application method of feed of grass carp nano controlled-release sodium butyrate in the present invention are as follows: by manufactured nano controlled-release sodium butyrate with
0.6% additive amount is mixed with feed, feeds grass carp.
Beneficial effects of the present invention: nanometer embedding is carried out to sodium butyrate using nanometer embedding techniques, there is easy to operate, reality
Convenient advantage is applied, and overcome sodium butyrate to have peculiar smell, easily deliquesced, it is difficult to utilized by enteron aisle, enteron aisle cannot be continuously, energy is provided
The shortcomings that amount.The health for promoting grass carp intestinal improves the absorption of nutriment, increases cultured output, improves grass carp cultivation
Economic benefit, have important scientific meaning and practical application value.
Detailed description of the invention:
Detailed description will be given by the following detailed implementation and drawings by the present invention for ease of explanation,.
Fig. 1 is grass carp production target performance map figure in the embodiment of the present invention 1；
Fig. 2 is grass carp production target performance map figure in the embodiment of the present invention 2；
Fig. 3 is grass carp intestinal height of naps/Crypt depth numerical value figure figure in the embodiment of the present invention 2；
Grass carp intestinal mucosa aspect graph in Fig. 4 embodiment of the present invention 2.
Specific embodiment:
Present embodiment uses following technical scheme: step 1: in sodium hydroxide solution, dropping evenly positive fourth
Acid reacts 1.5-2.0 hours after being added dropwise to complete, obtains butyric acid sodium solution, and wherein the mass ratio of sodium hydroxide and n-butyric acie is 1:1-
1.5；
Step 2: being added auxiliary material in the butyric acid sodium solution of step 1, and the mass ratio of auxiliary material and sodium butyrate is 1-1.5:2,
And the auxiliary material are as follows: 2-3 parts of beeswax, 1-2 parts of palm wax, fish oil 0.1-0.5,1-4 parts of polyethylene glycol, 0.5-1 parts of filler,
1-1.5 parts of nano controlled-release carrier, the nano controlled-release carrier is dried the preparation method comprises the following steps: 2.0054g is added in polymerization pipe
Macrogol 4000,3.00mL polycaprolactone and 0.2mL stannous octoate, liquid nitrogen is sudden cold, vacuumizes, and logical nitrogen drains oxygen in pipe
Gas is stirred to react 4h for 135 DEG C after tube sealing, product is transferred in small beaker, and ether precipitating is added, filters, filter residue dichloromethane
Alkane dissolution, is added methanol extraction, filters, dry 0.5h in vacuum drying oven；
Step 3: the mix products of butyric acid sodium solution and auxiliary material that step 2 obtains are spray-dried and are delayed up to nanometer
Sodium butyrate is released, drying temperature is 200-220 DEG C.
The application method of feed of grass carp nano controlled-release sodium butyrate in present embodiment are as follows: by manufactured nano controlled-release
Sodium butyrate is mixed with 0.6% additive amount with feed, feeds grass carp.
Embodiment 1: using feed of grass carp of the invention nano controlled-release sodium butyrate, carries out grass carp using following techniqueflow
Indoor culture obtains preferable effect.
(1) 40g sodium hydroxide is weighed, is dissolved in 1000ml water, 40g n-butyric acie is dropped evenly, reacts 2.0 after being added dropwise to complete
Hour, auxiliary material 150g is added, is spray-dried at 220 DEG C up to nano controlled-release sodium butyrate.
(2) auxiliary material is by 2 parts of beeswax, 2 parts of palm wax, 0.3 part of fish oil, 4 parts of polyethylene glycol, 0.8 part of filler, nano controlled-release
1.5 parts of carrier compositions.
(3) wherein nano controlled-release carrier the preparation method comprises the following steps: weigh the dry Macrogol 4000 of 2.0054g, be added
3.00mL polycaprolactone and 0.2mL stannous octoate, it is cooling in liquid nitrogen, it vacuumizes, logical nitrogen drains oxygen in pipe, and 135 DEG C are stirred
Reaction 4h is mixed, ether precipitating is added, filters, discards filtrate.Filter residue 100ml methylene chloride dissolves, and it is heavy that 200ml methanol is added
It forms sediment, dries 0.5h after suction filtration in vacuum drying oven.
(4) culture experiment is carried out in Changsha University's Research Base, and indoor circulation temperature-controlled glass aquarium specification is 120cm
× 80cm × 80cm adds 6.0g nano controlled-release fourth in per kilogram feed referring to the nutrition parameters and commercial feed formula of grass carp
Sour sodium.Feedstuff crushed 40 meshes, the grit through SLX-80 type soft-grain fodder-processing equipment academic title's diameter 2.5mm, natural
The feed of grass carp of addition nano controlled-release sodium butyrate is obtained after air-drying.
(5) raised in aquarium 30 tail original body mass be 6g, the consistent grass carp of size specification, feeding 7 weeks, each processing
If 3 repetitions, wherein not adding sodium butyrate is control group；It is one group of processing that Coating sodium butyrate is bought in market, and additive amount is
0.6%；Nano controlled-release sodium butyrate is processing three, additive amount 0.6%.It is fed daily in 8:30,12:30,16:30 time point,
It feeds altogether 3 times, feeding volume is the 4% of quality of fishes.Temperature of cultivation maintains 26 DEG C.
(6) water temperature, feeding volume and situation of ingesting are recorded daily.
(7) after cultivating, test production performance index, mainly calculate the average weight of every cylinder grass carp, rate of body weight gain, at
Motility rate, specific growth rate.Then random every cylinder takes 6 tail grass carps to calculate coefficient of condition.Referring to Fig.1, (Fig. 1 is grass carp production target
Can scheme figure) as can be seen from Figure 1, the nano controlled-release sodium butyrate of addition 6% in feed of grass carp, compared to the control group, culture efficiency is good, weight gain
Rate etc. all improves significantly, also improves compared to presently commercially available Coating sodium butyrate.
Embodiment 2: using feed of grass carp of the present invention nano controlled-release sodium butyrate, carries out grass carp net using following techniqueflow
Cage breeding is grown, and preferable effect is obtained.
(1) 40g sodium hydroxide is weighed, is dissolved in 1000ml water, 60g n-butyric acie is dropped evenly, reacts 2.0 after being added dropwise to complete
Hour, auxiliary material 200g is added, is spray-dried at 220 DEG C up to nano controlled-release sodium butyrate.
(2) for auxiliary material by 2.5 parts of beeswax, 1.5 parts of palm wax, 0.5 part of fish oil, 3 parts of polyethylene glycol, filler 1 part, nanometer are slow
Release 1.5 parts of carrier compositions.
(3) wherein nano controlled-release carrier the preparation method comprises the following steps: weigh the dry Macrogol 4000 of 2.0054g, be added
3.00mL polycaprolactone and 0.2mL stannous octoate, it is cooling in liquid nitrogen, it vacuumizes, logical nitrogen drains oxygen in pipe, and 135 DEG C are stirred
Reaction 4h is mixed, ether precipitating is added, filters, discards filtrate.Filter residue 100ml methylene chloride dissolves, and it is heavy that 200ml methanol is added
It forms sediment, dries 0.5h after suction filtration in vacuum drying oven.
(4) culture experiment carries out in Wangcheng cage culture base, and cage size is 200cm × 200cm × 250cm, reference
The nutrition parameters and commercial feed formula of grass carp add 6.0g nano controlled-release sodium butyrate in per kilogram feed.Feedstuff is through powder
Broken 40 mesh of mistake, the grit through SLX-80 type soft-grain fodder-processing equipment academic title's diameter 3.0mm obtain addition nanometer after natural air drying
It is sustained the feed of grass carp of sodium butyrate.
(5) 50 tail original body mass of raising are about 20g in each net cage, the consistent grass carp of size specification, and feeding 60 days, each
Processing sets 3 repetitions, wherein not adding sodium butyrate is control group；It is one group of processing that Coating sodium butyrate is bought in market, and additive amount is
0.6%；Nano controlled-release sodium butyrate is processing three, additive amount 0.6%.It is fed daily in 8:30,12:30,16:30 time point,
It feeds altogether 3 times, feeding volume is to increase with quality of fishes, and feeding volume is the 5% of quality of fishes within first week, is increased weekly later
1%, it cultivates under natural conditions.
(6) weather, water temperature, feeding volume and situation of ingesting are recorded daily.
(7) after cultivating, test production performance index, mainly calculate the average weight of every net cage grass carp, rate of body weight gain,
Survival rate, specific growth rate, then random every net cage takes 6 tail grass carps to calculate coefficient of condition, and Fig. 2 is grass carp production target performance map
Figure.
(8) each repetition takes 6 tail fishes at random, isolates enteron aisle, cleans intestinal contents with physiological saline, fixed after weighing
Position promesenteron then be fixed in 4% paraformaldehyde solution, routinely histotomy program be dehydrated, paraffin embedding, slice
It is dyed with H-E, micro- sem observation is taken pictures, and in 40 times of amplification of slice, every randomly selects 10 and moves towards straight and intact
Villus measures height of naps and adjacent Crypt depth, and calculates height of naps/Crypt depth.(Fig. 3 is grass carp intestinal villus
Highly/Crypt depth numerical value figure figure)；(Fig. 4 is grass carp intestinal Mucosa Morphology figure).
Find out from Fig. 2 and Fig. 3 and Fig. 4, the nano controlled-release sodium butyrate of outdoor cage culture feed of grass carp addition 6% is compared
Control group, culture efficiency is good, and rate of body weight gain etc. all improves significantly, improves villus proliferation, also has compared to presently commercially available Coating sodium butyrate
Certain raising.
The above shows and describes the basic principles and main features of the present invention and the advantages of the present invention.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and improvements may be made to the invention, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (1)
1. a kind of feed of grass carp preparation method of nano controlled-release sodium butyrate, it is characterised in that: it specific is the preparation method comprises the following steps: step
Rapid one: in sodium hydroxide solution, n-butyric acie is dropped evenly, is reacted 1.5-2.0 hours after being added dropwise to complete, obtains butyric acid sodium solution,
Wherein the mass ratio of sodium hydroxide and n-butyric acie is 1:1-1.5；Step 2: it is added in the butyric acid sodium solution that step 1 obtains auxiliary
Material, the mass ratio of auxiliary material and sodium butyrate are 1-1.5:2, and the auxiliary material are as follows: 2-3 parts of beeswax, 1-2 parts of palm wax, fish oil
0.1-0.5 parts, 1-4 parts of polyethylene glycol, 0.5-1 parts of filler, 1-1.5 parts of nano controlled-release carrier, the nano controlled-release carrier
The preparation method comprises the following steps: the dry Macrogol 4000 of 2.0054g is added in polymerization pipe, 3.00mL polycaprolactone and 0.2mL are pungent
Sour stannous, liquid nitrogen is sudden cold, vacuumizes, and logical nitrogen drains oxygen in pipe, is stirred to react 4h for 135 DEG C after tube sealing, product is transferred to
In small beaker, ether precipitating is added, filters, filter residue is dissolved with methylene chloride, and methanol extraction is added, and is filtered, and is done in vacuum drying oven
Dry 0.5h；Step 3: the product of step 2 be spray-dried up to nano controlled-release sodium butyrate, drying temperature 200-220
℃。
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| CN106561998A (en) * | 2016-11-14 | 2017-04-19 | 赵清国 | Micro-capsule coating sodium butyrate and preparation method thereof |
| CN107156535A (en) * | 2017-05-12 | 2017-09-15 | 广东诚生物科技有限公司 | A kind of premixed feed for feed of grass carp |
| CN110973369A (en) * | 2019-12-13 | 2020-04-10 | 湖南普菲克生物科技有限公司 | Slow-release sodium butyrate suitable for aquatic animals and preparation method thereof |
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| CN1404835A (en) * | 2001-06-28 | 2003-03-26 | 徐荣祥 | Composition for repairing and promoting regeneration of gastrointestinal tract mucous tissue and its preparation method thereof |
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