CN105995152A - Preparation method of traditional Chinese medicine micro-ecological feed additive for laying hens at egg laying later stage - Google Patents
Preparation method of traditional Chinese medicine micro-ecological feed additive for laying hens at egg laying later stage Download PDFInfo
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Abstract
The invention relates to a preparation method of a traditional Chinese medicine micro-ecological feed additive for laying hens at an egg laying later stage. The preparation method comprises the following steps of taking traditional Chinese medicines including forsythia suspensa, astragalus membranaceus, radix codonopsis, Chinese angelica, radix isatidis, motherworts, purple perilla, spina date seeds, radix ophiopogonis and licorice roots, performing superfine grinding on the traditional Chinese medicines, adding an alcohol solution, performing boiling over with a strong fire, performing heat preservation with a soft fire, performing cooling, performing filtration, concentrating filtrate, adding white sugar, performing boiling over, performing cooling to normal temperature, inoculating lactobacilli, red tea funguses and bifidobacteria, performing fermentation until white fungus blocks grow, and performing low-temperature concentration on bacterial liquid; and then weighing modified starch, arabic gum, cyclodextrin and syrup, adding the weighed modified starch, the weighed arabic gum, the weighed cyclodextrin and the weighed syrup to the concentrated bacterial liquid, performing stirring during adding, after uniform mixing and stirring, performing high-pressure homogenizing, and performing spray drying so as to obtain the traditional Chinese medicine micro-ecological feed additive. The traditional Chinese medicine micro-ecological feed additive disclosed by the invention has the efficacies of increasing the number of beneficial bacterium groups in intestinal tracts, enhancing immune functions, strengthening stress resistance of chicken bodies, strengthening disease resistance of the chicken bodies, increasing egg production and the like. Compared with a control group, the traditional Chinese medicine micro-ecological feed additive disclosed by the invention has the advantages that the egg laying rate is increased by 4.86% and 7.27%, the feed-egg rate is reduced by 7.65% and 5.82%, and the rate of soft and broken eggs is reduced by 23.64% and 31.72%; feeds are saved, the cost is reduced, and the economic benefits are increased.
Description
Technical field
The invention belongs to field of feed, particularly relate to the preparation method of a kind of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive.
Background technology
Along with the fast development of modern society and improving constantly of people's living standard, egg has become as requisite protein source in each family life.The method using high-energy, high protein, high-copper high ferro high calcium etc. in order to improve the speed of growth in the preparation of feedstuff has been the most universal, but also bring some problems to laying hen, such as dyspepsia, metabolic dysfunction etc., reduce the price of deed, cause laying hen dead, breeding layer chicken industry is produced huge economic loss.
Feedstuff is extremely critical for chicken body health and the increase egg production in laying hen later stage, in view of problems such as the prevention of disease during laying hen late growing stage and controls in prior art, occur in that some feed additives, but these products add mainly hormone medicine or other additives, such as antibiotic, hormone, high copper additives etc., see it is to solve some problems in a short time, but actually result in Egg Quality drastically to decline, it is often more important that can be to the healthy public harm causing tool big of people；It addition, these additives of life-time service can cause the Drug resistance of chicken, and it is unfavorable for digesting and assimilating of laying hen, the problems such as palatability is poor.
Chinese invention patent CN103815142A provides a kind of special feed improving laying hen later stage production performance and digestive function, described special feed comprises Semen Maydis 42%～66% by mass percentage, Semen Tritici aestivi 2%～24%, bean cake 5%～22%, fish flour 0.5%～2%, wheat bran 1%～5%, Zein powder 0.5%～5%, stone powder 6%～10%, calcium hydrogen phosphate 0.1%～3%, methionine 0.01%～0.2%, Sal 0.2%～0.4%, attapulgite clay 1%～3%, emulsifying agent 0.01%～0.1%, compound enzymic preparation 0.05%～0.1%, composite animal health-care agent 0.5%～2% and vitamin and microelement pre-mix material 1%.The special feed of the present invention can supplement the endogenous digestive enzyme of later stage laying hen of laying eggs, and extends the time of staying of chyme, regulation laying hen intestinal microecology balance, maintains intestinal health, improves laying hen later stage digestive function, improves production performance and fanning economics.Although the enzyme preparation of adding, do not use Chinese medicine and carry out fermenting and reduce the incidence rate of disease.
nullChinese invention patent CN 103431169A provides a kind of laying hen later stage feedstuff，Belong to technical field of animal，The feedstuff eaten particularly to a kind of laying hen being suitable for more than 56 week old，The raw material of composition feedstuff is according to following weight: Semen Maydis: 65 parts、Bean cake: 18 parts、Cottonseed meal: 4.6 parts、Hydrogen calcium: 2 parts、Stone powder: 8.5 parts、0.5 part of Oleum Glycines、0.05 part of the many ore deposits of laying hen、Lysine: 0.3 part、Methionine: 0.2 part、Laying hen multidimensional: 0.04 part、Concentrated Cod-liver Oil: 0.1 part、Antioxidant: 0.02 part、Stress be peaceful: 0.02 part、Phytase: 0.02 part、Choline: 0.05 part、Compound enzyme: 0.1 part、Probiotics: 0.05 part、Acidulant: 0.1 part、Sal: 0.25 part、Sodium bicarbonate: 0.1 part；Laying hen later stage feedstuff proportioning advantages of simple in the present invention, needed for being fully able to meet laying hen late growing stage, the beneficially healthy growth in laying hen later stage and the prolongation of laying period.Simply various nutritional labelings are carried out proportioning, adds enzyme preparation and probiotics, the most do not use Chinese medicine, more Chinese medicine is not fermented through microorganism, reach the common effect of existing Chinese medicine and probiotic bacteria.
During intensive animal feeding, Chinese herbal medicine replaces antibiotic to effectively reduce drug residue problem during animal feeding, and the appearance of biofermentation traditional Chinese medicine technology, the drug effect making Chinese herbal medicine improves 4-20 times, and gradually shows more significantly effect at aspects such as prevention disease and raising production performances.
Accordingly, it would be desirable to a kind of growth promoter that can meet laying hen early stage, good palatability, promoting laying hen early stage healthy growth, can improve again egg quality, the new feedstuff that will not work the mischief health is to solve existing issue.
Summary of the invention
Present invention aims to the deficiency of above technology, solve raising of current laying hen later stage egg production, antibiotic remains and the problem of anti-stress, thering is provided the preparation method of a kind of Egg Production of Laying Hens later stage feedstuff, the method is simple, it is easy to use to have, green safety, noresidue, pure ecology, viable bacteria content are high.
For reaching above-mentioned purpose, the technical solution used in the present invention is as follows:
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, it is characterized in that: it is to prepare according to the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 120-140 of following weight portion, Radix Astragali 120-140, Radix Codonopsis 120-140, Radix Angelicae Sinensis 100-120, Radix Isatidis 100-120, Herba Leonuri 100-120, Folium Perillae 80-100, Semen Ziziphi Spinosae 80-100, Radix Ophiopogonis 80-100, Radix Glycyrrhizae 40-60, each Study on Supermicro-pulverization of Traditional Chinese Drugs is added ethanol solution intense fire boil, slow fire is incubated, cold filtration, concentrated filtrate, add white sugar to boil, cool down to inoculating lactobacillus after room temperature, red tea fungus and bacillus bifidus, through 96 ~ 120h fermentation to growing white truffle, by bacterium solution cryoconcentration；Two, microcapsule is coated: weigh the microcapsule wall material modified starch 45-55 of following weight portion, arabic gum 15-20, beta-schardinger dextrin-15-20, syrup 25-30, join in the bacterium solution that 400-500kg concentrates, stirring while adding, after mixing and stirring, high pressure homogenize, it is spray-dried, obtains Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 4%-8%.
The preparation method of described a kind of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, it is characterized in that: weigh the Chinese medicine of weight portion in proportion, by its micronizing, the ethanol solution of 80%～the 90% of addition Chinese medicine gross weight 6～8 times amount, soak 30～60min, intense fire boils, slow fire insulation 30～60min, cools down Floor 12 filtered through gauze；Filtering residue adds 4～the ethanol solution of the 60% of 6 times amount～70% again, intense fire boils, slow fire insulation 30～60min, cooling Floor 12 filtered through gauze, merge twice filtrate, concentration and recovery ethanol, concentrates without stopping during ethanol with in ethanol detector detection concentrated solution, adds water and make every milliliter of water crude drug Han 0.25g in concentrated solution.
The preparation method of described a kind of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, it is characterized in that: in units of 100 Kg concentrated solutions, add white sugar 10～20Kg, and boil 3～5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 20～25Kg, setting fermentation temperature 35～38 DEG C, standing for fermentation cultivates 96～120h, growing white truffle to pH5.5 ~ 6.5, cryoconcentration, make every gram containing 0.5 gram of crude drug.
The preparation method of described a kind of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, it is characterised in that: the pressure 10-20MPa of high pressure homogenize, time 3-5min.
The preparation method of described a kind of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, it is characterised in that: spraying feeding temperature 50-60 DEG C, feed rate 15-20L/min, inlet temperature 185-190 DEG C, leaving air temp 60-70 DEG C.
The preparation method of described a kind of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, it is characterised in that: step one is carried out in aseptic fermenting cellar.
Feedstuff of the present invention has profitable strain quantity in increase intestinal, improves immunologic function, strengthens chicken body anti-stress, premunition, increase egg production, improves the effects such as qualified egg rate, and preparation method is simple, easy to use, noresidue.The beneficial effects of the present invention is and improve essential amino acids content in egg, 1 is the results are shown in Table: aspartic acid, glutamic acid, leucine account for the percentage ratio of total amino acids total amount and be respectively 9.32%, 12.11%, 9.89% as can be seen from Table 1, and above-mentioned three kinds of aminoacid improve 2-3 times than in commercially available egg through city's agricultural product quality inspection Spot detection.
Table 1 assay statement
Beneficial effects of the present invention is good to the prevention effect of Pullorum Disease and newcastle disease, and control rate reaches 95.0%, meanwhile, also has certain preventive effect and viable bacteria content high to coccidiosis, and content of microorganisms is 5.85 × 10 after measured9Cfu/g, the survival rate of the microorganism after high temperature granulating is more than 90%.
The viable bacteria content of the Chinese medicine feed additive probiotics of the present invention is high, the aseptic 0.1g preparation that weighs, and adds in 10ml diluent, fully shake up, make 10 times of serial dilutions, take last dilution factor 1ml, instill on selectivity agar culture plate, each dilution factor, make 3 plates, and with the coating of L-shaped glass rod uniformly, put and constant incubator is cultivated under the conditions of 37 DEG C 24～48h, cultivation expires and observes each plate colony growth situation, and counts.By the content of slaking test detection probiotic bacteria, determine optimal capsule diameter；After testing after fermentation, after dried, high temperature does not embeds granulation, high temperature embedding pelletize after, viable bacteria content after high temperature granulating commercially available, commercially available the results are shown in Table 2.
Table 2 viable bacteria content test results report
| Group | Viable count (cfu/g) |
| After fermentation | 3.2×108cfu/g |
| After drying | 3.5×1010cfu/g |
| High temperature does not embeds granulation | 1.7×105cfu/g |
| High temperature embedding is pelletized | 4.3×107cfu/g |
| Commercially available | 1.3×1010cfu/g |
| After commercially available high temperature granulating | 1.5×105cfu/g |
After fermenting as can be seen from Table 2, medicinal liquid viable count content is 3.2 × 108Cfu/g, after low-temperature vacuum drying, viable bacteria content is 3.5 × 1010Cfu/g, this is consistent with dried yield (recovery rate 10%), does not embeds the viable count content after pelletizing 1.7 × 10 through high temperature5After cfu/g, with commercially available high temperature granulating, viable count is suitable, and the viable count after high temperature granulating after embedding is 2.1 × 107-4.3×107Cfu/g exceeds two orders of magnitude than the viable count not embedded, and reaches every gram of viable count more than million, thus, solve the commercially available microbial ecological agent unstable problem to high temperature.
Beneficial effects of the present invention alleviates salpingitis tubal reaction to a certain extent, has good repair, tumor necrosis factor TNF-alpha and epidermal growth factor EGFR to reduce 43.62% and 18.59% compared with matched group respectively the damage of laying hen fallopian tube.
TNF-α and the impact of EGFR content in present invention laying hen brown on Luo Man oviduct tissue, experimental chicken is Luo Man brown shell layer 400 plumage, body weight 2000～2200g, 4 groups are divided at random by it, it is divided into matched group, 4% interpolation group, 5% interpolation group, 6% interpolation group, often organize each 100 plumages, experimental period from the beginning of within 56 weeks, feeding, until terminate for 72 weeks.Laying hen 60 week old end, respectively repeats to randomly draw 5 chickens and carries out cuing open killing, aseptic separation oviduct tissue, accurately weighs oviduct tissue 1g, adds 2mlPBS solution, after ice bath homogenate, and 4 DEG C of 12000r/min, centrifugal 10min, take supernatant standby.Use ELISA kit to measure TNF-α and EGFR content in oviduct tissue, the results are shown in Table 3.
TNF-α and the impact of EGFR content in table 3 feedstuff of the present invention laying hen brown on Luo Man oviduct tissue
| Index | Matched group | 4% interpolation group | 5% interpolation group | 6% interpolation group |
| TNF-α | 52.78±3.08 | 42.78±5.16 | 29.76±3.53 | 40.66±3.64 |
| EGFR | 189.73±5.63 | 171.23±6.24 | 156.45±5.83 | 162.56±8.73 |
Whether the health of laying hen oviduct tissue directly affects laying rate and Egg Quality.Being a kind of commonly encountered diseases of laying hen by bacterial hens salpingitis such as escherichia coli, You Yikai term and egg-laying peak are more typically.What this test was selected is the laying hen of early stage of laying eggs, and result shows, has the infiltration of substantial amounts of neutrophilic granulocyte and lymphocyte, cell damage, there occurs inflammatory reaction in matched group laying hen oviduct tissue.Test group layer diets adds the feedstuff of 5% present invention reduce inflammation to a certain extent reaction, especially preferable with 5% group of effect, after using this compound preparation, the damage of laying hen fallopian tube there is is good repair.
Beneficial effects of the present invention improves laying rate 7.27% and 4.68%, reduces feedstuff-egg ratio 7.65% and 5.82%, reduces soft rate of broken eggs 23.64% and 31.72%, has saved feedstuff, reduced cost, added economic benefit.
The impact of present invention performance in layers brown on Luo Man, test chicken is Luo Man brown shell layer 200 plumage, body weight 2000～2300g, divides 2 groups at random by it, matched group and test group, often organize 100 plumages, the laying hen early-stage fodder of control group fed market purchasing, test group feeds the feedstuff of the present invention, feedstuff inside hopper is all removed totally in just trying the 1st day phase 06:00, then feeding experiment daily ration, timing at per weekend ramming material, weighs, and adds up the actual feed consumption rate of each repetition.Record egg number, egg size, feed intake, broken shell number, soft-shelled egg number every day, calculate test laying hen laying rate, feedstuff-egg ratio and soft rate of broken eggs weekly, the results are shown in Table 4.
The impact of table 4 feedstuff of the present invention performance in layers brown on Luo Man
Chinese herbal medicine contains hormonelike bioactive substance, can regulate the growth performance of laying hen, reproductive capability and immunologic mechanism.Probiotic bacteria has regulating intestinal canal colony balance, improves the effect such as feed intake and laying rate.In this test, daily ration adds probiotic bacteria and Chinese herbal medicine simultaneously, laying rate of laying hen can be significantly improved, reduce feedstuff-egg ratio and soft rate of broken eggs.
Detailed description of the invention
Embodiment
1
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 120 of following weight portion, the Radix Astragali 120, Radix Codonopsis 120, Radix Angelicae Sinensis 100, Radix Isatidis 100, Herba Leonuri 100, Folium Perillae 80, Semen Ziziphi Spinosae 80, Radix Ophiopogonis 80, Radix Glycyrrhizae 40, by the ethanol solution of the 80% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 6 times amount, soak 30min, intense fire boils, slow fire insulation 30min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 60% of 4 times amount again, and intense fire boils, slow fire insulation 30min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 10Kg is added in units of 100 Kg concentrated solutions, and boil 3min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 20Kg, setting fermentation temperature 35 DEG C, standing for fermentation cultivates 96h, growing white truffle to pH5.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 45 of following weight portion, arabic gum 15, beta-schardinger dextrin-15, syrup 25, join 400kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 10MPa, time 3min, is spray-dried, and sets feeding temperature 50 DEG C, feed rate 15L/min, inlet temperature 185 DEG C, leaving air temp 60 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, by 4% basal diet adding laying hen to.
Embodiment
2
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 120 of following weight portion, the Radix Astragali 120, Radix Codonopsis 120, Radix Angelicae Sinensis 100, Radix Isatidis 100, Herba Leonuri 100, Folium Perillae 80, Semen Ziziphi Spinosae 80, Radix Ophiopogonis 80, Radix Glycyrrhizae 40, by the ethanol solution of the 80% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 6 times amount, soak 60min, intense fire boils, slow fire insulation 30min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 70% of 4 times amount again, and intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 12Kg is added in units of 100 Kg concentrated solutions, and boil 4min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 21Kg, setting fermentation temperature 36 DEG C, standing for fermentation cultivates 108h, growing white truffle to pH6.0, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 48 of following weight portion, arabic gum 16, beta-schardinger dextrin-16, syrup 26, join 410kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 11MPa, time 3min, is spray-dried, and sets feeding temperature 51 DEG C, feed rate 16L/min, inlet temperature 186 DEG C, leaving air temp 61 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, by 5% basal diet adding laying hen to.
Embodiment
3
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 120 of following weight portion, the Radix Astragali 120, Radix Codonopsis 120, Radix Angelicae Sinensis 100, Radix Isatidis 100, Herba Leonuri 100, Folium Perillae 80, Semen Ziziphi Spinosae 80, Radix Ophiopogonis 80, Radix Glycyrrhizae 40, by the ethanol solution of the 80% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 6 times amount, soak 30min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 60% of 5 times amount again, and intense fire boils, slow fire insulation 30min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 14Kg is added in units of 100 Kg concentrated solutions, and boil 5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 22Kg, setting fermentation temperature 37 DEG C, standing for fermentation cultivates 110h, growing white truffle to pH6.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 47 of following weight portion, arabic gum 17, beta-schardinger dextrin-17, syrup 27, add 420kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 14MPa, time 5min, is spray-dried, and sets feeding temperature 55 DEG C, feed rate 17L/min, inlet temperature 187 DEG C, leaving air temp 70 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, by 6% basal diet adding laying hen to.
Embodiment
4
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 120 of following weight portion, the Radix Astragali 120, Radix Codonopsis 120, Radix Angelicae Sinensis 100, Radix Isatidis 100, Herba Leonuri 100, Folium Perillae 80, Semen Ziziphi Spinosae 80, Radix Ophiopogonis 80, Radix Glycyrrhizae 40, by the ethanol solution of the 80% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 6 times amount, soak 60min, intense fire boils, slow fire insulation 30min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 70% of 5 times amount again, and intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 16Kg is added in units of 100 Kg concentrated solutions, and boil 3min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 23Kg, setting fermentation temperature 38 DEG C, standing for fermentation cultivates 120h, growing white truffle to pH6.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weighs the microcapsule wall material modified starch 48 of following weight portion, arabic gum 18, beta-schardinger dextrin-18, syrup 28, adds purified water 430kg, adds Traditional Chinese drug microecology extract 108kg while stirring, after mixing and stirring, high pressure homogenize, pressure 16MPa
Time 3-5min, is spray-dried, and sets feeding temperature 60 DEG C, feed rate 18L/min, inlet temperature 188 DEG C, leaving air temp 70 DEG C, cools, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, by 7% basal diet adding laying hen to.
Embodiment
5
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 120 of following weight portion, the Radix Astragali 120, Radix Codonopsis 120, Radix Angelicae Sinensis 100, Radix Isatidis 100, Herba Leonuri 100, Folium Perillae 80, Semen Ziziphi Spinosae 80, Radix Ophiopogonis 80, Radix Glycyrrhizae 40, by the ethanol solution of the 80% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 6 times amount, soak 60min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 60% of 6 times amount again, and intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 18Kg is added in units of 100 Kg concentrated solutions, and boil 4min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 24Kg, setting fermentation temperature 35 DEG C, standing for fermentation cultivates 96h, growing white truffle to pH5.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 49 of following weight portion, arabic gum 19, beta-schardinger dextrin-19, syrup 29, join 450kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 18MPa, time 4min, is spray-dried, and sets feeding temperature 60 DEG C, feed rate 20L/min, inlet temperature 189 DEG C, leaving air temp 68 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 8%.
Embodiment
6
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 120 of following weight portion, the Radix Astragali 120, Radix Codonopsis 120, Radix Angelicae Sinensis 100, Radix Isatidis 100, Herba Leonuri 100, Folium Perillae 80, Semen Ziziphi Spinosae 80, Radix Ophiopogonis 80, Radix Glycyrrhizae 40, by the ethanol solution of the 80% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 6 times amount, soak 60min, intense fire boils, slow fire insulation 30min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 70% of 6 times amount again, and intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 20Kg is added in units of 100 Kg concentrated solutions, and boil 5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 25Kg, setting fermentation temperature 36 DEG C, standing for fermentation cultivates 96h, growing white truffle to pH6.0, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 50 of following weight portion, arabic gum 20, beta-schardinger dextrin-20, syrup 30, join 460kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 20MPa, time 5min, is spray-dried, and sets feeding temperature 60 DEG C, feed rate 20L/min, inlet temperature 190 DEG C, leaving air temp 70 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 4%.
Embodiment
7
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 120 of following weight portion, the Radix Astragali 120, Radix Codonopsis 120, Radix Angelicae Sinensis 100, Radix Isatidis 100, Herba Leonuri 100, Folium Perillae 80, Semen Ziziphi Spinosae 80, Radix Ophiopogonis 80, Radix Glycyrrhizae 40, by the ethanol solution of the 90% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 8 times amount, soak 60min, intense fire boils, slow fire insulation 30min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 60% of 4 times amount again, and intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 12Kg is added in units of 100 Kg concentrated solutions, and boil 3min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 20Kg, setting fermentation temperature 37 DEG C, standing for fermentation cultivates 108h, growing white truffle to pH6.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 51 of following weight portion, arabic gum 15, beta-schardinger dextrin-15, syrup 25, join 470kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 10MPa, time 3min, is spray-dried, and sets feeding temperature 50 DEG C, feed rate 15L/min, inlet temperature 185 DEG C, leaving air temp 60 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 5%.
Embodiment
8
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 120 of following weight portion, the Radix Astragali 120, Radix Codonopsis 120, Radix Angelicae Sinensis 100, Radix Isatidis 100, Herba Leonuri 100, Folium Perillae 80, Semen Ziziphi Spinosae 80, Radix Ophiopogonis 80, Radix Glycyrrhizae 40, by the ethanol solution of the 90% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 8 times amount, soak 60min, intense fire boils, slow fire insulation 30min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 70% of 4 times amount again, and intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 14Kg is added in units of 100 Kg concentrated solutions, and boil 4min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 20Kg, setting fermentation temperature 38 DEG C, standing for fermentation cultivates 120h, growing white truffle to pH6.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 52 of following weight portion, arabic gum 16, beta-schardinger dextrin-15-20, syrup 26, join 480kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 18MPa, time 4min, is spray-dried, and sets feeding temperature 54 DEG C, feed rate 16L/min, inlet temperature 186 DEG C, leaving air temp 62 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, by 6% basal diet adding laying hen to.
Embodiment
9
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 120 of following weight portion, the Radix Astragali 120, Radix Codonopsis 120, Radix Angelicae Sinensis 100, Radix Isatidis 100, Herba Leonuri 100, Folium Perillae 80, Semen Ziziphi Spinosae 80, Radix Ophiopogonis 80, Radix Glycyrrhizae 40, by the ethanol solution of the 90% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 8 times amount, soak 60min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 60% of 5 times amount again, and intense fire boils, slow fire insulation 30min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 16Kg is added in units of 100 Kg concentrated solutions, and boil 5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 22Kg, setting fermentation temperature 35 DEG C, standing for fermentation cultivates 96h, growing white truffle to pH5.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 53 of following weight portion, arabic gum 17, beta-schardinger dextrin-17, syrup 27, join 490kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 20MPa, time 5min, is spray-dried, and sets feeding temperature 60 DEG C, feed rate 120L/min, inlet temperature 190 DEG C, leaving air temp 70 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 7%.
Embodiment
10
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 120 of following weight portion, the Radix Astragali 120, Radix Codonopsis 120, Radix Angelicae Sinensis 100, Radix Isatidis 100, Herba Leonuri 100, Folium Perillae 80, Semen Ziziphi Spinosae 80, Radix Ophiopogonis 80, Radix Glycyrrhizae 40, by the ethanol solution of the 90% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 8 times amount, soak 30min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 70% of 5 times amount again, and intense fire boils 60min, cools down Floor 12 filtered through gauze, merge twice filtrate, concentration and recovery ethanol, concentrates without stopping during ethanol with in ethanol detector detection concentrated solution, adds water and make every milliliter of water crude drug Han 0.25g in concentrated solution.White sugar 16Kg is added in units of 100 Kg concentrated solutions, and boil 3min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 23Kg, setting fermentation temperature 36 DEG C, standing for fermentation cultivates 120h, growing white truffle to pH6.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 55 of following weight portion, arabic gum 18, beta-schardinger dextrin-18, syrup 27, join 490kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 14MPa, time 3min, is spray-dried, and sets feeding temperature 60 DEG C, feed rate 20L/min, inlet temperature 190 DEG C, leaving air temp 70 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 8%.
Embodiment
11
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 120 of following weight portion, the Radix Astragali 120, Radix Codonopsis 120, Radix Angelicae Sinensis 100, Radix Isatidis 100, Herba Leonuri 100, Folium Perillae 80, Semen Ziziphi Spinosae 80, Radix Ophiopogonis 80, Radix Glycyrrhizae 40, by the ethanol solution of the 90% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 8 times amount, soak 360min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 60% of 6 times amount again, and intense fire boils, slow fire insulation 30min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 18Kg is added in units of 100 Kg concentrated solutions, and boil 4min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 24Kg, setting fermentation temperature 37 DEG C, standing for fermentation cultivates 1080h, growing white truffle to pH6.0, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 55 of following weight portion, arabic gum 20, beta-schardinger dextrin-20, syrup 30, join 500kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 20MPa, time 4min, is spray-dried, and sets feeding temperature 60 DEG C, feed rate 19L/min, inlet temperature 186 DEG C, leaving air temp 70 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 4%.
Embodiment
12
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 120 of following weight portion, the Radix Astragali 120, Radix Codonopsis 120, Radix Angelicae Sinensis 100, Radix Isatidis 100, Herba Leonuri 100, Folium Perillae 80, Semen Ziziphi Spinosae 80, Radix Ophiopogonis 80, Radix Glycyrrhizae 40, by the ethanol solution of the 90% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 8 times amount, soak 30min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 70% of 6 times amount again, and intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 20Kg is added in units of 100 Kg concentrated solutions, and boil 5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 25Kg, setting fermentation temperature 38 DEG C, standing for fermentation cultivates 96h, growing white truffle to pH6.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 55 of following weight portion, arabic gum 20, beta-schardinger dextrin-20, syrup 30, join 400kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 20MPa, time 3min, is spray-dried, and sets feeding temperature 60 DEG C, feed rate 20L/min, inlet temperature 190 DEG C, leaving air temp 70 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 5%.
Embodiment
13
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 140 of following weight portion, the Radix Astragali 140, Radix Codonopsis 140, Radix Angelicae Sinensis 120, Radix Isatidis 120, Herba Leonuri 120, Folium Perillae 100, Semen Ziziphi Spinosae 100, Radix Ophiopogonis 100, Radix Glycyrrhizae 60, by the ethanol solution of the 80% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 6 times amount, soak 30min, intense fire boils, slow fire insulation 30min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 60% of 4 times amount again, and intense fire boils, slow fire insulation 30min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 10Kg is added in units of 100 Kg concentrated solutions, and boil 3min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 20Kg, setting fermentation temperature 35 DEG C, standing for fermentation cultivates 96h, growing white truffle to pH5.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 45 of following weight portion, arabic gum 15, beta-schardinger dextrin-15, syrup 25, join 410kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 10MPa, time 3min, is spray-dried, and sets feeding temperature 50 DEG C, feed rate 15L/min, inlet temperature 185 DEG C, leaving air temp 60 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 6%.
Embodiment
14
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 140 of following weight portion, the Radix Astragali 140, Radix Codonopsis 140, Radix Angelicae Sinensis 120, Radix Isatidis 120, Herba Leonuri 120, Folium Perillae 100, Semen Ziziphi Spinosae 100, Radix Ophiopogonis 100, Radix Glycyrrhizae 60, by the ethanol solution of the 80% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 6 times amount, soak 60min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 70% of 4 times amount again, and intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 12Kg is added in units of 100 Kg concentrated solutions, and boil 4min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 21Kg, setting fermentation temperature 36 DEG C, standing for fermentation cultivates 110h, growing white truffle to pH6.0, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 46 of following weight portion, arabic gum 16, beta-schardinger dextrin-16, syrup 26, join 420kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 12MPa, time 4min, is spray-dried, and sets feeding temperature 52 DEG C, feed rate 16L/min, inlet temperature 186 DEG C, leaving air temp 62 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 7%.
Embodiment
15
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 140 of following weight portion, the Radix Astragali 140, Radix Codonopsis 140, Radix Angelicae Sinensis 120, Radix Isatidis 120, Herba Leonuri 120, Folium Perillae 100, Semen Ziziphi Spinosae 100, Radix Ophiopogonis 100, Radix Glycyrrhizae 60, by the ethanol solution of the 80% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 6 times amount, soak 30min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 60% of 5 times amount again, and intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 14Kg is added in units of 100 Kg concentrated solutions, and boil 5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 22Kg, setting fermentation temperature 37 DEG C, standing for fermentation cultivates 120h, growing white truffle to pH6.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 47 of following weight portion, arabic gum 17, beta-schardinger dextrin-17, syrup 27, join 430kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 14MPa, time 5min, is spray-dried, and sets feeding temperature 54 DEG C, feed rate 17L/min, inlet temperature 187 DEG C, leaving air temp 64 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 8%.
Embodiment
16
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 140 of following weight portion, the Radix Astragali 140, Radix Codonopsis 140, Radix Angelicae Sinensis 120, Radix Isatidis 120, Herba Leonuri 120, Folium Perillae 100, Semen Ziziphi Spinosae 100, Radix Ophiopogonis 100, Radix Glycyrrhizae 60, by the ethanol solution of the 80% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 6 times amount, soak 60min, intense fire boils, slow fire insulation 30min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 70% of 5 times amount again, and intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 16Kg is added in units of 100 Kg concentrated solutions, and boil 3min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 23Kg, setting fermentation temperature 38 DEG C, standing for fermentation cultivates 96h, growing white truffle to pH5.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 48 of following weight portion, arabic gum 18, beta-schardinger dextrin-18, syrup 28, join 450kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 16MPa, time 4min, is spray-dried, and sets feeding temperature 56 DEG C, feed rate 18L/min, inlet temperature 188 DEG C, leaving air temp 60 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 4%.
Embodiment
17
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 140 of following weight portion, the Radix Astragali 140, Radix Codonopsis 140, Radix Angelicae Sinensis 120, Radix Isatidis 120, Herba Leonuri 120, Folium Perillae 100, Semen Ziziphi Spinosae 100, Radix Ophiopogonis 100, Radix Glycyrrhizae 60, by the ethanol solution of the 80% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 6 times amount, soak 60min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 60% of 6 times amount again, and intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 18Kg is added in units of 100 Kg concentrated solutions, and boil 4min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 24Kg, setting fermentation temperature 35 DEG C, standing for fermentation cultivates 120h, growing white truffle to pH6.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 49 of following weight portion, arabic gum 19, beta-schardinger dextrin-19, syrup 29, join 460kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 18MPa, time 4min, is spray-dried, and sets feeding temperature 60 DEG C, feed rate 20L/min, inlet temperature 190 DEG C, leaving air temp 70 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 5%.
Embodiment
18
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 140 of following weight portion, the Radix Astragali 140, Radix Codonopsis 140, Radix Angelicae Sinensis 120, Radix Isatidis 120, Herba Leonuri 120, Folium Perillae 100, Semen Ziziphi Spinosae 100, Radix Ophiopogonis 100, Radix Glycyrrhizae 60, by the ethanol solution of the 80% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 6 times amount, soak 30min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 70% of 6 times amount again, and intense fire boils, slow fire insulation 30min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 20Kg is added in units of 100 Kg concentrated solutions, and boil 5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 25Kg, setting fermentation temperature 38 DEG C, standing for fermentation cultivates 108h, growing white truffle to pH6.0, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 50 of following weight portion, arabic gum 20, beta-schardinger dextrin-20, syrup 30, join 470kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 20MPa, time 5min, is spray-dried, and sets feeding temperature 60 DEG C, feed rate 20L/min, inlet temperature 190 DEG C, leaving air temp 70 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 6%.
Embodiment
19
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 140 of following weight portion, the Radix Astragali 140, Radix Codonopsis 140, Radix Angelicae Sinensis 120, Radix Isatidis 120, Herba Leonuri 120, Folium Perillae 100, Semen Ziziphi Spinosae 100, Radix Ophiopogonis 100, Radix Glycyrrhizae 60, by the ethanol solution of the 90% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 8 times amount, soak 60min, intense fire boils, slow fire insulation 30min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 60% of 4 times amount again, and intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 10Kg is added in units of 100 Kg concentrated solutions, and boil 3min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 20Kg, setting fermentation temperature 35 DEG C, standing for fermentation cultivates 120h, growing white truffle to pH6.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 54 of following weight portion, arabic gum 15, beta-schardinger dextrin-15, syrup 25, join 480kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 10MPa, time 5min, is spray-dried, and sets feeding temperature 60 DEG C, feed rate 20L/min, inlet temperature 190 DEG C, leaving air temp 70 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 7%.
Embodiment
20
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 140 of following weight portion, the Radix Astragali 140, Radix Codonopsis 140, Radix Angelicae Sinensis 120, Radix Isatidis 120, Herba Leonuri 120, Folium Perillae 100, Semen Ziziphi Spinosae 100, Radix Ophiopogonis 100, Radix Glycyrrhizae 60, by the ethanol solution of the 90% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 8 times amount, soak 30min, intense fire boils, slow fire insulation 30min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 70% of 4 times amount again, and intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 12Kg is added in units of 100 Kg concentrated solutions, and boil 3min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 22Kg, setting fermentation temperature 38 DEG C, standing for fermentation cultivates 96h, growing white truffle to pH5.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 52 of following weight portion, arabic gum 17, beta-schardinger dextrin-17, syrup 27, join 480kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 14MPa, time 5min, is spray-dried, and sets feeding temperature 50 DEG C, feed rate 15L/min, inlet temperature 185 DEG C, leaving air temp 60 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 8%.
Embodiment
21
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 140 of following weight portion, the Radix Astragali 140, Radix Codonopsis 140, Radix Angelicae Sinensis 120, Radix Isatidis 120, Herba Leonuri 120, Folium Perillae 100, Semen Ziziphi Spinosae 100, Radix Ophiopogonis 100, Radix Glycyrrhizae 60, by the ethanol solution of the 90% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 8 times amount, soak 30min, intense fire boils, slow fire insulation 30min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 60% of 5 times amount again, and intense fire boils, slow fire insulation 30min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 16Kg is added in units of 100 Kg concentrated solutions, and boil 5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 23Kg, setting fermentation temperature 35 DEG C, standing for fermentation cultivates 118h, growing white truffle to pH6.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 53 of following weight portion, arabic gum 18, beta-schardinger dextrin-18, syrup 28, join 490kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 20MPa, time 3min, is spray-dried, and sets feeding temperature 60 DEG C, feed rate 20L/min, inlet temperature 190 DEG C, leaving air temp 70 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 4%.
Embodiment
22
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 140 of following weight portion, the Radix Astragali 140, Radix Codonopsis 140, Radix Angelicae Sinensis 120, Radix Isatidis 120, Herba Leonuri 120, Folium Perillae 100, Semen Ziziphi Spinosae 100, Radix Ophiopogonis 100, Radix Glycyrrhizae 60, by the ethanol solution of the 90% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 8 times amount, soak 30min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 70% of 5 times amount again, and intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 16Kg is added in units of 100 Kg concentrated solutions, and boil 3min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 23Kg, setting fermentation temperature 36 DEG C, standing for fermentation cultivates 110h, growing white truffle to pH6.0, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 55 of following weight portion, arabic gum 18, beta-schardinger dextrin-18, syrup 28, join 500kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 20MPa, time 3min, is spray-dried, and sets feeding temperature 60 DEG C, feed rate 20L/min, inlet temperature 190 DEG C, leaving air temp 70 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 5%.
Embodiment
23
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 140 of following weight portion, the Radix Astragali 140, Radix Codonopsis 140, Radix Angelicae Sinensis 120, Radix Isatidis 120, Herba Leonuri 120, Folium Perillae 100, Semen Ziziphi Spinosae 100, Radix Ophiopogonis 100, Radix Glycyrrhizae 60, by the ethanol solution of the 90% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 8 times amount, soak 30min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 60% of 6 times amount again, and intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 18Kg is added in units of 100 Kg concentrated solutions, and boil 4min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 24Kg, setting fermentation temperature 37 DEG C, standing for fermentation cultivates 110h, growing white truffle to pH6.0, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 45 of following weight portion, arabic gum 19, beta-schardinger dextrin-19, syrup 29, join 400kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 18MPa, time 4min, is spray-dried, and sets feeding temperature 60 DEG C, feed rate 18L/min, inlet temperature 190 DEG C, leaving air temp 70 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 6%.
Embodiment
24
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 140 of following weight portion, the Radix Astragali 140, Radix Codonopsis 140, Radix Angelicae Sinensis 120, Radix Isatidis 120, Herba Leonuri 120, Folium Perillae 100, Semen Ziziphi Spinosae 100, Radix Ophiopogonis 100, Radix Glycyrrhizae 60, by the ethanol solution of the 90% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 8 times amount, soak 60min, intense fire boils, slow fire insulation 30min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 70% of 6 times amount again, and intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 20Kg is added in units of 100 Kg concentrated solutions, and boil 5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 25Kg, setting fermentation temperature 38 DEG C, standing for fermentation cultivates 96h, growing white truffle to pH5.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 47 of following weight portion, arabic gum 20, beta-schardinger dextrin-20, syrup 30, join 440kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 16MPa, time 4min, is spray-dried, and sets feeding temperature 60 DEG C, feed rate 20L/min, inlet temperature 190 DEG C, leaving air temp 70 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 7%.
Embodiment
25
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 130 of following weight portion, the Radix Astragali 130, Radix Codonopsis 130, Radix Angelicae Sinensis 110, Radix Isatidis 110, Herba Leonuri 110, Folium Perillae 90, Semen Ziziphi Spinosae 90, Radix Ophiopogonis 90, Radix Glycyrrhizae 50, by the ethanol solution of the 80% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 7 times amount, soak 60min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 65% of 4 times amount again, and intense fire boils, slow fire insulation 40min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 11Kg is added in units of 100 Kg concentrated solutions, and boil 3.5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 25Kg, setting fermentation temperature 35 DEG C, standing for fermentation cultivates 120h, growing white truffle to pH5.8, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 50 of following weight portion, arabic gum 20, beta-schardinger dextrin-20, syrup 30, join 450kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 11MPa, time 3.5min, is spray-dried, and sets feeding temperature 53 DEG C, feed rate 16L/min, inlet temperature 185 DEG C, leaving air temp 63 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 8%.
Embodiment
26
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 130 of following weight portion, the Radix Astragali 130, Radix Codonopsis 130, Radix Angelicae Sinensis 110, Radix Isatidis 110, Herba Leonuri 110, Folium Perillae 90, Semen Ziziphi Spinosae 90, Radix Ophiopogonis 90, Radix Glycyrrhizae 50, by the ethanol solution of the 90% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 7 times amount, soak 60min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 70% of 5 times amount again, and intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 20Kg is added in units of 100 Kg concentrated solutions, and boil 5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 25Kg, setting fermentation temperature 37 DEG C, standing for fermentation cultivates 110h, growing white truffle to pH6.2, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 49 of following weight portion, arabic gum 19, beta-schardinger dextrin-19, syrup 27, join 470kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 17MPa, time 4.5min, is spray-dried, and sets feeding temperature 53 DEG C, feed rate 17L/min, inlet temperature 187 DEG C, leaving air temp 65 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 8%.
Embodiment
27
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 130 of following weight portion, the Radix Astragali 130, Radix Codonopsis 130, Radix Angelicae Sinensis 110, Radix Isatidis 110, Herba Leonuri 110, Folium Perillae 90, Semen Ziziphi Spinosae 90, Radix Ophiopogonis 90, Radix Glycyrrhizae 50, by the ethanol solution of the 80% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 7 times amount, soak 30min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 70% of 6 times amount again, and intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 17Kg is added in units of 100 Kg concentrated solutions, and boil 5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 24Kg, setting fermentation temperature 38 DEG C, standing for fermentation cultivates 120h, growing white truffle to pH6.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 52 of following weight portion, arabic gum 18, beta-schardinger dextrin-19, syrup 30, join 500kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 20MPa, time 5min, is spray-dried, and sets feeding temperature 60 DEG C, feed rate 20L/min, inlet temperature 190 DEG C, leaving air temp 70 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 7%.
Embodiment
28
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 130 of following weight portion, the Radix Astragali 130, Radix Codonopsis 130, Radix Angelicae Sinensis 110, Radix Isatidis 110, Herba Leonuri 110, Folium Perillae 90, Semen Ziziphi Spinosae 90, Radix Ophiopogonis 90, Radix Glycyrrhizae 50, by the ethanol solution of the 85% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 7 times amount, soak 60min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 65% of 5 times amount again, and intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 16Kg is added in units of 100 Kg concentrated solutions, and boil 4.5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 25Kg, setting fermentation temperature 38 DEG C, standing for fermentation cultivates 114h, growing white truffle to pH6.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 55 of following weight portion, arabic gum 17, beta-schardinger dextrin-18, syrup 28, join 450kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 20MPa, time 5min, is spray-dried, and sets feeding temperature 55 DEG C, feed rate 17L/min, inlet temperature 187 DEG C, leaving air temp 65 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 6%.
Embodiment
29
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 130 of following weight portion, the Radix Astragali 130, Radix Codonopsis 130, Radix Angelicae Sinensis 110, Radix Isatidis 110, Herba Leonuri 110, Folium Perillae 90, Semen Ziziphi Spinosae 90, Radix Ophiopogonis 90, Radix Glycyrrhizae 50, by the ethanol solution of the 85% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 7 times amount, soak 60min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 70% of 5 times amount again, and intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 20Kg is added in units of 100 Kg concentrated solutions, and boil 5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 25Kg, setting fermentation temperature 38 DEG C, standing for fermentation cultivates 96h, growing white truffle to pH5.6, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 52 of following weight portion, arabic gum 20, beta-schardinger dextrin-20, syrup 30, join 480kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 17MPa, time 4.5min, is spray-dried, and sets feeding temperature 57 DEG C, feed rate 17L/min, inlet temperature 188 DEG C, leaving air temp 63 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 6%.
Embodiment
30
A kind of preparation method of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 130 of following weight portion, the Radix Astragali 130, Radix Codonopsis 130, Radix Angelicae Sinensis 110, Radix Isatidis 110, Herba Leonuri 110, Folium Perillae 90, Semen Ziziphi Spinosae 90, Radix Ophiopogonis 90, Radix Glycyrrhizae 50, by the ethanol solution of the 85% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 7 times amount, soak 50min, intense fire boils, slow fire insulation 40min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 65% of 5 times amount again, and intense fire boils, slow fire insulation 50min, cooling Floor 12 filtered through gauze, merges twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g.White sugar 17Kg is added in units of 100 Kg concentrated solutions, and boil 3.5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 23Kg, setting fermentation temperature 38 DEG C, standing for fermentation cultivates 96h, growing white truffle to pH5.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 45 of following weight portion, arabic gum 20, beta-schardinger dextrin-20, syrup 25, join 455kg and concentrate in bacterium solution, stirring while adding, after mixing and stirring, high pressure homogenize, pressure 12.5MPa, time 4.5min, is spray-dried, and sets feeding temperature 53 DEG C, feed rate 16L/min, inlet temperature 187 DEG C, leaving air temp 63 DEG C, cool, obtain Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 4%.
Embodiment
31
A kind of preparation method of Egg Production of Laying Hens early stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 130 of following weight portion, the Radix Astragali 130, Radix Codonopsis 130, Radix Angelicae Sinensis 110, Radix Isatidis 110, Herba Leonuri 110, Folium Perillae 90, Semen Ziziphi Spinosae 90, Radix Ophiopogonis 90, Radix Glycyrrhizae 50, by the ethanol solution of the 85% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 7 times amount, soak 60min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 65% of 5 times amount again, intense fire boils, slow fire insulation 35min, cooling Floor 12 filtered through gauze, merge twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g, white sugar 18Kg is added in units of 100 Kg concentrated solutions, and boil 4.5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 22.5Kg, set fermentation temperature 38 DEG C, standing for fermentation cultivates 96h, growing white truffle to pH5.5, cryoconcentration, make every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 48 of following weight portion, arabic gum 18, beta-schardinger dextrin-20, syrup 30, join in the bacterium solution that 460kg concentrates, stirring while adding, after mixing and stirring, the pressure 15MPa of high pressure homogenize, time 3.5min, spraying feeding temperature 53 DEG C, feed rate 18L/min, inlet temperature 188 DEG C, leaving air temp 65 DEG C, cool, obtain Egg Production of Laying Hens early stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 5%.
Embodiment
32
A kind of preparation method of Egg Production of Laying Hens early stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 130 of following weight portion, the Radix Astragali 130, Radix Codonopsis 130, Radix Angelicae Sinensis 110, Radix Isatidis 110, Herba Leonuri 110, Folium Perillae 90, Semen Ziziphi Spinosae 90, Radix Ophiopogonis 90, Radix Glycyrrhizae 50, by the ethanol solution of the 90% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 6 times amount, soak 35min, intense fire boils, slow fire insulation 55min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 70% of 6 times amount again, intense fire boils, slow fire insulation 60min, cooling Floor 12 filtered through gauze, merge twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g, white sugar 16Kg is added in units of 100 Kg concentrated solutions, and boil 4.5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 22.5Kg, set fermentation temperature 37 DEG C, standing for fermentation cultivates 108h, growing white truffle to pH6.5, cryoconcentration, make every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 55 of following weight portion, arabic gum 15, beta-schardinger dextrin-15, syrup 25, join in the bacterium solution that 460kg concentrates, stirring while adding, after mixing and stirring, the pressure 17MPa of high pressure homogenize, time 3.5min, spraying feeding temperature 51 DEG C, feed rate 16L/min, inlet temperature 190 DEG C, leaving air temp 60 DEG C, cool, obtain Egg Production of Laying Hens early stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 7%.
Embodiment
33
A kind of preparation method of Egg Production of Laying Hens early stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 135 of following weight portion, the Radix Astragali 135, Radix Codonopsis 135, Radix Angelicae Sinensis 115, Radix Isatidis 115, Herba Leonuri 115, Folium Perillae 95, Semen Ziziphi Spinosae 95, Radix Ophiopogonis 95, Radix Glycyrrhizae 55, by the ethanol solution of the 85% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 7 times amount, soak 30min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 70% of 6 times amount again, intense fire boils, slow fire insulation 45min, cooling Floor 12 filtered through gauze, merge twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g, white sugar 20Kg is added in units of 100 Kg concentrated solutions, and boil 5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 25Kg, set fermentation temperature 35 DEG C, standing for fermentation cultivates 114h, growing white truffle to pH6.5, cryoconcentration, make every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 49 of following weight portion, arabic gum 19, beta-schardinger dextrin-20, syrup 30, join in the bacterium solution that 500kg concentrates, stirring while adding, after mixing and stirring, the pressure 20MPa of high pressure homogenize, time 3.5min, spraying feeding temperature 57 DEG C, feed rate 18L/min, inlet temperature 189 DEG C, leaving air temp 65 DEG C, cool, obtain Egg Production of Laying Hens early stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 8%.
Embodiment
34
A kind of preparation method of Egg Production of Laying Hens early stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 115 of following weight portion, the Radix Astragali 115, Radix Codonopsis 115, Radix Angelicae Sinensis 115, Radix Isatidis 115, Herba Leonuri 110, Folium Perillae 90, Semen Ziziphi Spinosae 90, Radix Ophiopogonis 90, Radix Glycyrrhizae 45, by the ethanol solution of the 90% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 8 times amount, soak 60min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 70% of 4 times amount again, intense fire boils, slow fire insulation 30～60min, cooling Floor 12 filtered through gauze, merge twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g, white sugar 20Kg is added in units of 100 Kg concentrated solutions, and boil 4.5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 25Kg, set fermentation temperature 38 DEG C, standing for fermentation cultivates 96h, growing white truffle to pH6.5, cryoconcentration, make every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 49 of following weight portion, arabic gum 17, beta-schardinger dextrin-16, syrup 28, joins in the bacterium solution that 500kg concentrates, stirring while adding, after mixing and stirring, the pressure 20MPa of high pressure homogenize, time 5min, feeding temperature 60 DEG C of spraying, feed rate 20L/min, inlet temperature 190 DEG C, leaving air temp 70 DEG C, cool, obtain Egg Production of Laying Hens early stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 7%.
Embodiment
35
A kind of preparation method of Egg Production of Laying Hens early stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 130 of following weight portion, the Radix Astragali 130, Radix Codonopsis 130, Radix Angelicae Sinensis 115, Radix Isatidis 115, Herba Leonuri 115, Folium Perillae 95, Semen Ziziphi Spinosae 95, Radix Ophiopogonis 95, Radix Glycyrrhizae 55, by the ethanol solution of the 85% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 7 times amount, soak 60min, intense fire boils, slow fire insulation 30min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 65% of 5 times amount again, intense fire boils, slow fire insulation 45min, cooling Floor 12 filtered through gauze, merge twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g, white sugar 17Kg is added in units of 100 Kg concentrated solutions, and boil 3.5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 25Kg, set fermentation temperature 38 DEG C, standing for fermentation cultivates 96h, growing white truffle to pH5.5, cryoconcentration, make every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 55 of following weight portion, arabic gum 20, beta-schardinger dextrin-20, syrup 30, join in the bacterium solution that 500kg concentrates, stirring while adding, after mixing and stirring, the pressure 19MPa of high pressure homogenize, time 4.5min, spraying feeding temperature 60 DEG C, feed rate 20L/min, inlet temperature 190 DEG C, leaving air temp 70 DEG C, cool, obtain Egg Production of Laying Hens early stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 6%.
Embodiment
36
A kind of preparation method of Egg Production of Laying Hens early stage Traditional Chinese drug microecology feed additive, comprise the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 130 of following weight portion, the Radix Astragali 130, Radix Codonopsis 130, Radix Angelicae Sinensis 110, Radix Isatidis 110, Herba Leonuri 110, Folium Perillae 90, Semen Ziziphi Spinosae 90, Radix Ophiopogonis 90, Radix Glycyrrhizae 50, by the ethanol solution of the 90% of each Study on Supermicro-pulverization of Traditional Chinese Drugs addition Chinese medicine gross weight 8 times amount, soak 30min, intense fire boils, slow fire insulation 60min, cools down Floor 12 filtered through gauze；Filtering residue adds the ethanol solution of the 65% of 5 times amount again, intense fire boils, slow fire insulation 45min, cooling Floor 12 filtered through gauze, merge twice filtrate, concentration and recovery ethanol, concentrate without stopping during ethanol with in ethanol detector detection concentrated solution, add water in concentrated solution and make every milliliter of water crude drug Han 0.25g, white sugar 20Kg is added in units of 100 Kg concentrated solutions, and boil 5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 22.5Kg, set fermentation temperature 38 DEG C, standing for fermentation cultivates 96h, growing white truffle to pH5.5, cryoconcentration, make every gram containing 0.5 gram of crude drug.Two, microcapsule is coated: weigh the microcapsule wall material modified starch 55 of following weight portion, arabic gum 20, beta-schardinger dextrin-20, syrup 30, join in the bacterium solution that 450kg concentrates, stirring while adding, after mixing and stirring, the pressure 17MPa of high pressure homogenize, time 3.5min, spraying feeding temperature 55 DEG C, feed rate 16L/min, inlet temperature 187 DEG C, leaving air temp 65 DEG C, cool, obtain Egg Production of Laying Hens early stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 8%.
The above; being only the detailed description of the invention of the present invention, but protection scope of the present invention is not limited thereto, any those familiar with the art is in the technical scope that the invention discloses; change can be readily occurred in or replace, all should contain within protection scope of the present invention.
Claims (6)
1. the preparation method of an Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, it is characterized in that: it is to prepare according to the following steps: one, Traditional Chinese drug microecology extract: weigh the Fructus Forsythiae 120-140 of following weight portion, Radix Astragali 120-140, Radix Codonopsis 120-140, Radix Angelicae Sinensis 100-120, Radix Isatidis 100-120, Herba Leonuri 100-120, Folium Perillae 80-100, Semen Ziziphi Spinosae 80-100, Radix Ophiopogonis 80-100, Radix Glycyrrhizae 40-60, each Study on Supermicro-pulverization of Traditional Chinese Drugs is added ethanol solution intense fire boil, slow fire is incubated, cold filtration, concentrated filtrate, add white sugar to boil, cool down to inoculating lactobacillus after room temperature, red tea fungus and bacillus bifidus, through 96 ~ 120h fermentation to growing white truffle, by bacterium solution cryoconcentration；Two, microcapsule is coated: weigh the microcapsule wall material modified starch 45-55 of following weight portion, arabic gum 15-20, beta-schardinger dextrin-15-20, syrup 25-30, join in the bacterium solution that 400-500kg concentrates, stirring while adding, after mixing and stirring, high pressure homogenize, it is spray-dried, obtains Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive, add in laying hens essential ration by 4%-8%.
The preparation method of a kind of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive the most according to claim 1, it is characterized in that: weigh the Chinese medicine of weight portion in proportion, by its micronizing, the ethanol solution of 80%～the 90% of addition Chinese medicine gross weight 6～8 times amount, soak 30～60min, intense fire boils, slow fire insulation 30～60min, cools down Floor 12 filtered through gauze；Filtering residue adds 4～the ethanol solution of the 60% of 6 times amount～70% again, intense fire boils, slow fire insulation 30～60min, cooling Floor 12 filtered through gauze, merge twice filtrate, concentration and recovery ethanol, concentrates without stopping during ethanol with in ethanol detector detection concentrated solution, adds water and make every milliliter of water crude drug Han 0.25g in concentrated solution.
The preparation method of a kind of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive the most according to claim 1, it is characterised in that: with 100
Kg concentrated solution is that unit adds white sugar 10～20Kg, and boil 3～5min, it is cooled to room temperature, inoculating lactobacillus, red tea fungus and bacillus bifidus (commercially available) preparation 20～25Kg, setting fermentation temperature 35～38 DEG C, standing for fermentation cultivates 96～120h, growing white truffle to pH5.5 ~ 6.5, cryoconcentration, makes every gram containing 0.5 gram of crude drug.
The preparation method of a kind of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive the most according to claim 1, it is characterised in that: the pressure 10-20MPa of high pressure homogenize, time 3-5min.
The preparation method of a kind of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive the most according to claim 1, it is characterised in that: spraying feeding temperature 50-60 DEG C, feed rate 15-20L/min, inlet temperature 185-190 DEG C, leaving air temp 60-70 DEG C.
The preparation method of a kind of Egg Production of Laying Hens later stage Traditional Chinese drug microecology feed additive the most according to claim 1, it is characterised in that: step one is carried out in aseptic fermenting cellar.
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| CN108740306A (en) * | 2018-05-03 | 2018-11-06 | 武汉环绿时代生态园林工程有限公司 | A kind of feed addictive and its feeding method |
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