CN105963355A - Medicine for preventing and treating senile dementia - Google Patents
Medicine for preventing and treating senile dementia Download PDFInfo
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- CN105963355A CN105963355A CN201610414334.2A CN201610414334A CN105963355A CN 105963355 A CN105963355 A CN 105963355A CN 201610414334 A CN201610414334 A CN 201610414334A CN 105963355 A CN105963355 A CN 105963355A
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- industrial hemp
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- senile dementia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/60—Moraceae (Mulberry family), e.g. breadfruit or fig
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
Abstract
The invention discloses a medicine for preventing and treating senile dementia. The medicine is prepared by mixing 0.3-99.7 parts of industrial hemp semen cannabis extract and 0.3-99.7 parts of industrial hemp cannabinoid to be uniform. The pharmaceutical composition has an obvious relieving effect on spatial cognition disorder and study and memory function decline caused by senile dementia and cerebral ischemia, the content of inhibitory amino acid aspartic acid, glycine and gamma-aminobutyric acid on the cerebral cortex of a senile dementia rat can be increased, the toxicity of excitable glutamic acid can be reduced, generated beta-AP in AD rat hippocampus can be reduced, and an obvious relieving effect is achieved for memory function decline caused by cerebral ischemia; mouse dysmnesia caused by NaNO2 can be obviously relieved. All effects are superior to those of cannabidiol. The medicine has a definitely remarkable effect on preventing and treating senile dementia and has good application and popularization prospects.
Description
Technical field
The invention belongs to curing senile dementia drug world, concretely relate to a kind of with industrial hemp for the most former
The pharmaceutical composition of the senile dementia prevention and cure of material preparation.
Background technology
The plantation of Fructus Cannabis is with a long history, and ancient times, Fructus Cannabis was mainly used in fabrication and processing rope, fishing net, clothing and paper making raw material,
And oils and fats, food etc..Development and progress along with society, it has been found that containing a kind of toxic component (tetrahydrochysene Fructus Cannabis in Fructus Cannabis
Phenol) people can be made to cause unreal addiction, American-European many countries once forbade cultivating Fructus Cannabis within considerably long period.Economic use value due to Fructus Cannabis
Height, to the eighties in 20th century, low toxicity Hemp Varieties the plantation that puts it over have been cultivated in the research of some European countries.Nineteen ninety Europe
Coenosarc takes the lead in Emergency fixes agricultural policy, has abolished the ban of Fructus Cannabis of forbidding cultivating, has started to recover the production of Fructus Cannabis and research.Beautiful subsequently
The states such as state, Canada, Australia relieve the ban of Fructus Cannabis plantation, and whole world Fructus Cannabis cultivated area and fiber production have had rapidly
Increasing, the exploitation of industrial hemp are started by American-European countries again, and the demand of ecological Fructus Cannabis is also being increased rapidly by international market
Long.According to the economic attribution of Fructus Cannabis, servicing for the mankind for making full use of it, within 1988, the United Nations's clear stipulaties does not possess extraction poison
Property composition (tetrahydrocannabinol THC) be worth or suck directly as drugs, specialize in the raw material Fructus Cannabis of industrial use, industrial hemp
(the tetrahydrocannabinol content in its trophophase Fructus Cannabis floral leaf is less than 0.3%), legal can carry out implantation in large scale and industrialization
Develop.
Industrial hemp be show unique characteristics, living resources that comparative advantages are prominent.Industrial hemp has with conventional toxic Fructus Cannabis
Essential distinction, industrial hemp is the raw material of industry product of a class Non-toxic, has high economic use value.At the end of 2003
Countries in the world successively select altogether 25 industrial hemp kinds, and seven states such as the method for European Union, moral, English the most all become industry greatly
Fiber crops mainly plant manufacturing country, with industrial hemp fibers and fiber crops seed and flower, leaf, root, stem for raw material carry out series of products research and development with
Industrialization comprehensive exploitation, processes major product jute skin fiber, stalk core fibre, hemp seed oil fat and seed dregs of rice egg in industrial hemp primary
In vain, medicinal standard extract realize industrialization produce on the basis of, carry out deep processing and utilization, the product derived from further
Reached more than 25,000 kinds, contain the clothing of the mankind, food, shelter, row, with each big series products.The research of industrial hemp industry,
Exploitation and production are concentrated mainly on the technology developed countries such as Europe, Canada, the U.S..The development of industrial hemp industry, be first
Or the low industrial hemp new varieties that poison nontoxic from selection-breeding start, and achieve the plantation of scale and high skill based on this
The comprehensive development and utilization of art industrialization, defines one " emerging pollution-free industry group " and the quick growth point of infant industry economy.
Fructus Cannabis is Fructus Cannabis dry mature fruit, is generally used as medicine by Fructus Cannabis ancient times, and dietetic therapy is the most on the books.Health ministry
Fructus Cannabis is included in " being medicine and food " " medicine-food two-purpose " list.The property of medicine of Fructus Cannabis and pharmacology be: sweet,
Flat.Return spleen, stomach, large intestine channel.Function cures mainly: moisturize, and laxation is treating stranguria, invigorates blood circulation.Control dryness of the intestine constipation, quench one's thirst, pyretic stranguria, migratory arthralgia, dysentery
Disease.Menoxenia, scabies, tinea leprosy.Those benefits in detail is had to record in medical book, such as " herbal classic ": " invigorating the spleen and replenishing QI.”；Tang Materia Medica:
" main five kinds of over strain.”；" Handbook of Prescriptions for Emergencies ": " control extreme thirst, day eclipse number bucket, hot urination person: pockmarks one liter, three liters of water, boil three, four boilings, extracting juice
Drink it.”；" Japan hanako materia medica ": " qi-restoratives labor, long muscle, stimulating milk secretion, only quench one's thirst, expedite the emergence of.Control perverse and unreasonable manner to produce.”；Supplement to the Herbal: under "
Gas, diuresis, go migratory arthralgia skin stupid, fry and make perfume (or spice) smash to pieces, urine leaching juice clothes；Married woman's footling presentation gulps down two or seven pieces.”；" Treatise on Dietetic Therapy ":
" extracting juice is cooked congee, and goes the five internal organs wind, lung moistening.Control that joint is obstructed, deal with, promoting blood circulation.”.
The equal hyoscine of floral leaf of Fructus Cannabis.The property of medicine of Folium Cannabis and pharmacology be: pungent；Poisonous.Return lung；Bladder；Large intestine channel.Function master
Control: pain relieving, Dingchuan, drive ascarid.Cure mainly asthma, fall and flutter pain, ascariasis.The property of medicine of Flos Cannabis and pharmacology be: bitter；Pungent；Warm in nature；Have
Poison.Function cures mainly: dispel the wind；Invigorate blood circulation；Hair growth promoting.Main air disease numb limbs and tense tendons；Pruritus all over；Women's amenorrhea.
Senile dementia is to occur at senilism phase and the senile disordered brain function being characterized with progressive dementia and produces
Raw intelligence damage syndrome.Including alzheimer disease------Alzheimer (Alzheimer ' s Disease AD) and
Vascular dementia (Vascular dementia, VD) two classes.AD is the constitutional cerebral nerve that a kind of polyphyly factor causes
Degeneration, with acetylcholine esterase AchE activity reduction, monoamines, the change etc. of content of amino acids neurotransmitter.VD is then
How closely related with the cerebral lesion that cerebral ischemia repeatedly or cerebral infarction cause.It is acquired that old dementia patients shows as cerebral cortex
Senior obstacle, including memory, language, feels and the progressive injury of the function such as motion, and is often accompanied by personality, personality and emotion control
The exception of the aspects such as system.
In recent years, domestic and international clinical research shows, acetylcholinesteraseinhibitors inhibitors is the treatment maximally effective medicine of AD, additionally
The treatment of the diseases such as glaucoma, myasthenia gravis, intestinal obstruction is also had preferably effect (Jin Youyu. pharmacology's (the 5th edition),
2001；54-55.).The acetylcholinesteraseinhibitors inhibitors of at present FDA approval (acetylocholinesterase inhibitor,
ACEhI) there are tacrine (tacrine) donepezil (donepezil), galantamine (galanthamine) and huperzine A,
This kind of medicine activity by acetylcholine esterase inhibition, improves levels of acetylcholine, strengthens the merit that intracerebroventricuacetylcholine acetylcholine is neural
Can, improve the state of an illness of AD early stage patient to a certain extent.
But above-mentioned inhibitor still suffers from certain defect, mainly relatively big to the toxicity of liver, there is the secondary work of nausea and vomiting
With and internal acetylcholine availability low etc..
Cannabidiol (CBD) is that a kind of nontoxic of extraction from Fructus Cannabis floral leaf can be used for medicine, cosmetics, health food
The aldehydes matter of a kind of high added value.At present, Israel, the U.S., Britain etc. are sent out, and reach country and have done raw material with it and developed
Multiple special effect medicine and cosmetics.CBD is the non-additive composition in Fructus Cannabis, and THC can be hindered to affect nerve system of human body, and
There is the pharmacologically actives such as spasmolytic, resisting rheumatoid arthritis, anxiety, also have the report in terms for the treatment of mental sickness.Fructus Cannabis
Diphenol is the most on the books in terms for the treatment of senile dementia, but its therapeutic effect is unsatisfactory.Therefore developing new activity high, poison is secondary
The medicine acting on little treatment AD seems the most necessary.
Summary of the invention
Present invention aims to the deficiencies in the prior art, it is provided that a kind of prepare with industrial hemp for primary raw material
The pharmaceutical composition of senile dementia prevention and cure.
The purpose of the present invention is achieved by the following technical programs.
Except as otherwise noted, percent of the present invention is mass percent.
The medicine of a kind of senile dementia prevention and cure, it is characterised in that be made up of the raw material blending of following weight parts: industry is big
Fiber crops Fructus Cannabis extract 0.3 part～99.7 parts, industrial hemp Urtica cannabina L. element 99.7 parts～0.3 part.
The raw material composition of described medicine is preferably industrial hemp Fructus Cannabis extract 40 parts and industrial hemp Urtica cannabina L. element 60 parts.
Described industrial hemp Fructus Cannabis extract is prepared by the following method and forms:
(1) ripe industrial hemp Fructus Cannabis is taken, drying, remove impurity, standby after pulverizing；
(2) under the conditions of 20～85 DEG C, with ethanol to pulverize Fructus Cannabis material extract, concentration of alcohol be 95%～
100% (V/V), solid-liquid ratio is 1:5～1:20；
(3) leach lixiviating solution, after concentrating under reduced pressure, be industrial hemp Fructus Cannabis extract.
When industrial hemp Fructus Cannabis being extracted with ethanol, following various ways can be used to carry out: as soaked under room temperature
Carry, every batch materials extraction 2 times, each 7～10 days；Under the conditions of 60～85 DEG C, heating extraction 2 times, each 1～3 hour；Ultrasonic
Ripple assisted extraction, ultrasonic frequency 30～60kHz, power 100～1000W, extraction time 30～60min, extraction temperature 25～
50℃；Also other prior aries such as microwave radiation exaraction can be used.
Described industrial hemp Urtica cannabina L. element is prepared by the following method and forms:
(1) take the flower of industrial hemp, leaf, fiber crops bran or the mixture of three, drying, remove impurity, be crushed to 10～60 mesh；
(2) supercritical carbon dioxide extraction: the above-mentioned industrial hemp raw material crushed is put into supercritical carbon dioxide extraction
During fetching is put, control extraction temperature 40～50 DEG C, extraction time 30～90min, extracting pressure 25～35Mpa, carbon-dioxide flow
Amount 40kg/h, extract is collected in separating still outlet；
(3) the extract ethanol elution of 95%～100% (V/V) of 0.2～10 times of volume, washing steps is 1～3
Secondary；
(4) eluent is collected, concentrating under reduced pressure, vacuum drying, i.e. obtain industrial hemp Urtica cannabina L. element after pulverizing.
The flower of described industrial hemp, leaf, the optimum ratio of fiber crops bran three's mixture are 1:3:2.
Common Folium Cannabis, Flos Cannabis are respectively provided with toxicity (containing the extremely unreal material of tetrahydrocannabinol THC marijuana hemp).For row
Except the toxicity of crude drug, present invention preferably employs the flower of industrial hemp kind " cloud fiber crops No. 1 " of Yunnan growth, leaf, fiber crops bran
With Fructus Cannabis as raw material.By China's relevant legal documents regulation, " cloud fiber crops No. 1 " can only be planted within the border in Yunnan, and its flower,
Leaf, fiber crops bran can only be processed within the border in Yunnan.
The pharmaceutical composition of the present invention can make different pharmaceutical preparation, including oral agents and injection, wherein mouth further
Taking agent and include capsule, oral liquid, tablet, drop pill, granule etc., injection includes injection dosage form and freeze-dried powder injection
Type etc..When preparing oral formulations, available auxiliary type agent can be that starch, dextrin or cyclodextrin, sucrose, stearate etc. are normal
Rule filler.Lyophilized injectable powder can be prepared by methods such as aseptic spray drying, low-temperature vacuum drying, lyophilizations.Each system
The later stage preparation technology of agent and equipment all belong to the routine techniques of pharmaceutical field, and this is not construed as limiting by the present invention, therefore at this most in detail
State.
Spatial cognition obstacle that senile dementia and cerebral ischemia are caused by the pharmaceutical composition of the present invention and learning and memory merit
The effect that improves significantly can be declined, alzheimer disease rat cerebral cortex inhibitory aminoacid aspartic acid, sweet ammonia can be increased
Acid and the content of γ-aminobutyric acid, reduce the toxicity of irritability glutamic acid, and can reduce the product of β-AP in AD Rat hippocampus
Raw, the learning and memory function decline causing cerebral ischemia is obviously improved effect；NaNO can also be obviously improved2Induced mice
Dysmnesia.Each action effect is superior to cannabidiol.The medicine of the present invention has the most significantly effect to senile dementia prevention and cure
Really, there is good popularizing application prospect.
Detailed description of the invention
Below by embodiment, the present invention is described in further detail, but embodiment is not to the technology of the present invention side
The restriction of case.
Embodiment 1
Use flower, leaf, fiber crops bran and the Fructus Cannabis of industrial hemp kind " cloud fiber crops No. 1 " of Yunnan growth as raw material.
Take maturation industrial hemp Fructus Cannabis, drying, remove impurity, standby after pulverizing；With ethanol to pulverize Fructus Cannabis material extract (
Under the conditions of 60～85 DEG C, heating extraction 2 times, each 1～3 hour), concentration of alcohol is 95%～100% (V/V), and solid-liquid ratio is
1:5～1:20；Leach lixiviating solution, after concentrating under reduced pressure, be industrial hemp Fructus Cannabis extract.
Take the flower of industrial hemp, leaf, the mixture of fiber crops bran 1:3:2 mass ratio, drying, remove impurity, be crushed to 10～60 mesh；
Supercritical carbon dioxide extraction: the above-mentioned industrial hemp raw material crushed is put in supercritical carbon dioxide extraction apparatus, control
Extraction temperature 40 processed～50 DEG C, extraction time 30～90min, extracting pressure 25～35Mpa, carbon dioxide flow 40kg/h, point
Extract is collected from still outlet；The extract ethanol elution of 95%～100% (V/V) of 0.2～10 times of volume, washing steps
It it is 1～3 time；Collect eluent, concentrating under reduced pressure, vacuum drying, after pulverizing, obtain industrial hemp Urtica cannabina L. element.
Take industrial hemp Fructus Cannabis extract 40 parts, industrial hemp Urtica cannabina L. element 60 parts, mixing, obtain required preventing and treating senile dementia
The medicine B of disease.
Embodiment 2
Repeat embodiment 1, have following difference: when industrial hemp Fructus Cannabis being extracted with ethanol, soak under room temperature
Carry, every batch materials extraction 2 times, each 7～10 days.Take the flower of industrial hemp, leaf, the mixture conduct of fiber crops bran 1:1:1 mass ratio
The extraction raw material of industrial hemp Urtica cannabina L. element.
Take industrial hemp Fructus Cannabis extract 0.3 part, industrial hemp Urtica cannabina L. element 99.7 parts, mixing, obtain required preventing and treating old
The medicine A of dementia.
Embodiment 3
Repeat embodiment 1, have following difference: when industrial hemp Fructus Cannabis being extracted with ethanol, use ultrasonic
Ripple assisted extraction, ultrasonic frequency 30～60kHz, power 100～1000W, extraction time 30～60min, extraction temperature 25～
50℃.Take the flower of industrial hemp, leaf, fiber crops bran 3:2:1 mass ratio mixture as industrial hemp Urtica cannabina L. element extraction raw material.
Take industrial hemp Fructus Cannabis extract 99.7 parts, industrial hemp Urtica cannabina L. element 0.3 part, mixing, obtain required preventing and treating old
The medicine C of dementia.
Embodiment 4
Example 1 gained medicine 100 grams (crosses 80 mesh sieves), adds 60 grams of microcrystalline Cellulose, crosses 80 mesh sieve three times, mixing
Uniformly, spray into 95% ethanol solution, soft material processed, cross 40 mesh sieves and pelletize, 60 DEG C of dry half an hour, be sub-packed in 3# capsule, plastic-aluminum
Composite packaging, prepares capsule.
Embodiment 5
Example 1 gained medicine, admixture doses 5～the dried starch of 20% and 1～the magnesium stearate etc. of 5%, through mixed
Close, pelletize, be dried, tabletting, prepare tablet.
Embodiment 6
The adjuvants such as Example 1 gained medicine, adds sucrose water and the preservative of convention amount, stabilizer.Filtration, sterilizing,
It is distributed in 10mL bottle, makes oral liquid.
Embodiment 7
Example 1 gained medicine, add water for injection dissolve, add 2.0 ‰ activated carbons, stirring, filter, continue with
0.45 μm, 0.22 μm microporous filter membrane classified filtering, supplement water for injection, be sub-packed in cillin bottle, lyophilization, recharge high-purity
Nitrogen, jumps a queue, gland, packaging, prepares injection.
The Application Example 1 medicine of the present invention preventive and therapeutic effect to experimental AD
1, impact (the Morris water maze row that alzheimer disease animal model spatial cognition obstacle is improved by medicine of the present invention
For learning experiment)
Choose body weight 300-380g SD rat, male and female half and half, use Ibotenic acid (IBO) to activate thing senile silly
Slow-witted model, modeling method with reference to relevant document (Xu Shuyun waits chief editor. pharmacological experimental methodology, the third edition. people's health is published
Society.2002:848.Hu Jingqing, Lai Shilong, etc. China's geriatrics magazine 2000；19 (2): 5-6).After model builds up, will deposit
Live animal is randomly divided into 6 groups: operative control group group；Model control group；Cannabidiol group；Medicine A, B, C group of the present invention.The respectively company of group
Continuous gavage 2 months.Hereafter carry out Morris water maze Behaviors survey, continuous 6 days, test and be divided into two stages: (1) 1-5 days
Trained rat；Within (2) the 6th days, carrying out relevant Behaviors survey, result see table.
Table 1: the impact that alzheimer disease animal model spatial cognition obstacle is improved by the present invention
Note: compare with model group, * p ＜ 0.05, * * p ＜ 0.01, * * * P ＜ 0.001；△ P ＜ is compared with cannabidiol group
0.05。
Above-mentioned test result indicate that: the spatial cognition obstacle of AD rat is improved significantly by medicine A, B, C group of the present invention
Effect, compares with model group and has significant difference (P ＜ 0.05～0.001), and effect is better than cannabidiol group.
Impact that 2, alzheimer disease animal memory dysfunction is improved by the present invention (rat keeps away camera bellows experiment: Xu Shuyun,
Deng chief editor. pharmacological experimental methodology, the third edition. People's Health Publisher .2002:827.)
By above-mentioned laboratory animal after being administered 2 months, first experiment is placed in rat and keeps away in camera bellows camera-lucida, when rat enters
After camera bellows, shock by electricity 1 second, then take out animal, after 1 week, repeat experiment, record rat by camera-lucida enter time of camera bellows and number of times,
Number, result such as following table.
Table 2: the impact that alzheimer disease animal memory dysfunction is improved by the present invention
Note: compare with model group, * p ＜ 0.05, * * p ＜ 0.01, * * * P ＜ 0.001；Compare with cannabidiol group, △ P ＜
0.05。
Result shows, the memory dysfunction of AD rat is improved significantly by medicine of the present invention, with model group ratio
More all having significant difference (P ＜ 0.05～0.001), cannabidiol also has certain effect, but effect is not as good as the present invention.
3, the present invention impact on alzheimer disease rat cerebral cortex tissue homogenate amino acid neurotransmitter.
After above-mentioned experiment terminates, take the ultrasonic homogenate of rat cerebral cortex, survey amino acid neurotransmitter by HPLC method
Content, result is as follows.
Table 3: the present invention impact on alzheimer disease rat cerebral cortex tissue homogenate amino acid neurotransmitter
Note: compare with model group, * p ＜ 0.05；Compare with cannabidiol group, △ P ＜ 0.05.
Result shows, compares with model group, and the rising of experimental AD rat cerebral cortex glutamic acid is had by medicine of the present invention
Significantly inhibitory action, can increase AD rat cerebral cortex inhibitory aminoacid aspartic acid, glycine and γ-aminobutyric acid
Content, has significance (P ＜ 0.05) through statistical procedures difference.Comparing with cannabidiol group, the present invention is to experimental AD rat
The inhibitory action that cerebral cortex glutamic acid raises, and increase AD rat cerebral cortex inhibitory aminoacid aspartic acid, sweet ammonia
The effect of acid and alpha-aminobutyric acid content is superior to cannabidiol.
4, the present invention impact on alzheimer disease rat brain hippocampal homogenates beta amyloid peptide (β-AP).
After experiment terminates, taking the ultrasonic homogenate of rat brain Hippocampus, with putting the content exempting from method survey β-AP, result is as follows:
Table 4: the present invention impact on alzheimer disease rat brain hippocampal homogenates β AP
Note: compare with model group, * * p ＜ 0.01, * * * p ＜ 0.001；Compare with cannabidiol group, △ P ＜ 0.05, △ △
P ＜ 0.01.
Result shows, compares with model group, and the rising of β-AP in experimental AD rat brain tissue is had by medicine of the present invention
Significantly inhibitory action, has significance (P ＜ 0.00～0.001) through statistical procedures difference.Compare with cannabidiol group, this
Bright to the inhibitory action of the rising of β-AP in experimental AD rat brain tissue more than cannabidiol, have through statistical procedures difference
Significance (P ＜ 0.05～0.01).
Application Example 2 present invention impact on experimental vascular dementia (VD) learning and memory function.
Choose body weight 260～290g male Wistar rat, be placed in and keep away in camera bellows camera-lucida, after rat enters camera bellows, electricity
Hit 1 second, then take out animal.The body weight intraperitoneal anesthesia of secondary daily 10% chloral hydrate (0.3ml/100g), is fixed on surgical plate,
Prior to head opening, expose atlas transverse process wing aperture, burn bilateral vertebral artery with electric cautery, cause permanent occlusion,
Skin suture.Climbing over rat, expose and dissociate bilateral common carotid arteries, closes bilateral common carotid arteries 5min with arteriole folder folder, then fills
Note 1h, 3 times repeatedly, Ischemia Reperfusion, causes serious learning memory disorder, postoperative suture muscles, skin, intramuscular injection penicillin 2 days
Infection (Zhu Yu edits. the disease model of laboratory animal. and Tianjin Scientific English Translation publishing house .1997 page 304).Experiment is with 60
Rat, is randomly divided into 6 groups, often group 10, i.e. Sham-operated control group；Model control group；Cannabidiol group；A, B, C group of the present invention.
Second day after operation starts medication, successive administration 10 days, latter 1 hour of last administration, is sequentially placed into by rat and keeps away camera bellows camera-lucida
In, each one, in recording 5 minutes, entering the number of times of camera bellows, number of elements and incubation period, result is as follows.
Table 5: the impact that vascular dementia animal memory dysfunction is improved by the present invention
Note: compare with model group, * p ＜ 0.05, * * p ＜ 0.01；Compare with cannabidiol group, △ P ＜ 0.05.
Result shows, compares with model group, and medicine group of the present invention enters the number of times of camera bellows and substantially reduces in 5 minutes, hide
Phase substantially increases, and has significance (P ＜ 0.05～0.01) through statistical procedures difference.Compare with cannabidiol group, of the present invention group 5
The number of times entering camera bellows in minute is higher than cannabidiol group less than cannabidiol group, incubation period.Conclusion: cerebral ischemia is drawn by the present invention
The learning and memory function decline risen is significantly improved, and effect is better than cannabidiol.
The present invention is to NaNO for Application Example 32The amnemonic impact of induced mice
Choosing body weight 18～22g Kunming mouse 60, male and female half and half, be randomly divided into 6 groups, often group 10, the most right
According to group；Model control group；Cannabidiol group；A, B, C group of the present invention.Each group gastric infusion is after 7 days, in addition to Normal group each group
Subcutaneous injection NaNO immediately after training2120mg/kg, each group is administered in latter 24 hours lasts of training, and 1h enters after being administered
Row step down test, records incubation period and errors number that animal is made mistakes.Result is as follows:
Table 6: the present invention is to NaNO2The amnemonic impact of induced mice
Note: compare with model group, * * p ＜ 0.01, * * * p ＜ 0.001；Compare with cannabidiol group, △ P ＜ 0.05.
Result shows, compares with model group, and of the present invention group to NaNO2Induced mice dysmnesia all improve significantly work
With, the number of times that mice is made mistakes can be significantly reduced, extend the incubation period that mice is made mistakes, have significance through statistical procedures difference
(P ＜ 0.05～0.01).Comparing with cannabidiol, the present invention is to NaNO2The amnemonic improvement result of induced mice, reduce little
Number of times that Mus is made mistakes and extend the preclinical effect that mice makes mistakes and be superior to cannabidiol, has through statistical procedures difference
Significance (P ＜ 0.05).
By above-mentioned experiment, we demonstrate that the spatial cognition obstacle that senile dementia and cerebral ischemia are caused by the present invention
Decline, with learning and memory function, the effect that all improves significantly, alzheimer disease rat cerebral cortex inhibitory aminoacid can be increased
Glycine and the content of γ-aminobutyric acid, reduce the toxicity of irritability glutamic acid, and can reduce β-AP in AD Rat hippocampus
Generation, the learning and memory function that cerebral ischemia is caused decline be obviously improved effect；NaNO can also be obviously improved2Caused
Memory deficits in mice.Each action effect is superior to cannabidiol.
Claims (6)
1. the medicine of a senile dementia prevention and cure, it is characterised in that be made up of the raw material blending of following weight parts: industrial hemp
Fructus Cannabis extract 0.3 part～99.7 parts, industrial hemp Urtica cannabina L. element 99.7 parts～0.3 part.
The medicine of senile dementia prevention and cure the most according to claim 1, it is characterised in that: the raw material of described medicine consists of
Industrial hemp Fructus Cannabis extract 40 parts and industrial hemp Urtica cannabina L. element 60 parts.
The medicine of senile dementia prevention and cure the most according to claim 1, it is characterised in that: described industrial hemp Fructus Cannabis
Extract is prepared by the following method and forms:
(1) ripe industrial hemp Fructus Cannabis is taken, drying, remove impurity, standby after pulverizing；
(2) under the conditions of 20～85 DEG C, extracting pulverizing Fructus Cannabis material with ethanol, concentration of alcohol is 95%～100% (V/
V), solid-liquid ratio is 1:5～1:20；
(3) leach lixiviating solution, after concentrating under reduced pressure, be industrial hemp Fructus Cannabis extract.
The medicine of senile dementia prevention and cure the most according to claim 1, it is characterised in that: described industrial hemp Urtica cannabina L. element
It is prepared by the following method and forms:
(1) take the flower of industrial hemp, leaf, fiber crops bran or the mixture of three, drying, remove impurity, be crushed to 10～60 mesh；
(2) supercritical carbon dioxide extraction: the above-mentioned industrial hemp raw material crushed is put into supercritical carbon dioxide extraction dress
In putting, control extraction temperature 40～50 DEG C, extraction time 30～90min, extracting pressure 25～35Mpa, carbon dioxide flow
40kg/h, extract is collected in separating still outlet；
(3) the extract ethanol elution of 95%～100% (V/V) of 0.2～10 times of volume, washing steps is 1～3 time；
(4) eluent is collected, concentrating under reduced pressure, vacuum drying, obtain industrial hemp Urtica cannabina L. element after pulverizing.
The medicine of senile dementia prevention and cure the most according to claim 4, it is characterised in that: the flower of described industrial hemp,
Leaf, the optimum ratio of fiber crops bran three's mixture are 1:3:2.
6. according to the medicine of the senile dementia prevention and cure described in claim 1 or 2 or 3 or 4 or 5, it is characterised in that: described work
The kind of sparetime university fiber crops is preferably " cloud fiber crops No. 1 ".
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111743936A (en) * | 2020-07-28 | 2020-10-09 | 云南玉麻生物科技有限公司 | Functional hemp chewing gum for improving memory and reducing pressure and preparation method thereof |
| CN113973778A (en) * | 2021-12-15 | 2022-01-28 | 大连医科大学附属第一医院 | Method for preventing and treating senile dementia and improving learning and memory based on terahertz wave intervention |
| CN114042099A (en) * | 2021-09-29 | 2022-02-15 | 大通汉麻生物科技研究院(青岛)有限公司 | anti-Parkinson-dementia syndrome cyclolepine pair and preparation method thereof |
Citations (1)
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| CN105505565A (en) * | 2015-12-28 | 2016-04-20 | 贵州航天乌江机电设备有限责任公司 | Method for extracting industrial hemp oil rich in cannabidiol |
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| CN105505565A (en) * | 2015-12-28 | 2016-04-20 | 贵州航天乌江机电设备有限责任公司 | Method for extracting industrial hemp oil rich in cannabidiol |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111743936A (en) * | 2020-07-28 | 2020-10-09 | 云南玉麻生物科技有限公司 | Functional hemp chewing gum for improving memory and reducing pressure and preparation method thereof |
| CN114042099A (en) * | 2021-09-29 | 2022-02-15 | 大通汉麻生物科技研究院(青岛)有限公司 | anti-Parkinson-dementia syndrome cyclolepine pair and preparation method thereof |
| CN113973778A (en) * | 2021-12-15 | 2022-01-28 | 大连医科大学附属第一医院 | Method for preventing and treating senile dementia and improving learning and memory based on terahertz wave intervention |
| CN113973778B (en) * | 2021-12-15 | 2023-01-17 | 大连医科大学附属第一医院 | Method for preventing and treating senile dementia and improving learning and memory based on terahertz wave intervention |
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Application publication date: 20160928 |