CN113973778B - Method for preventing and treating senile dementia and improving learning and memory based on terahertz wave intervention - Google Patents
Method for preventing and treating senile dementia and improving learning and memory based on terahertz wave intervention Download PDFInfo
- Publication number
- CN113973778B CN113973778B CN202111533230.0A CN202111533230A CN113973778B CN 113973778 B CN113973778 B CN 113973778B CN 202111533230 A CN202111533230 A CN 202111533230A CN 113973778 B CN113973778 B CN 113973778B
- Authority
- CN
- China
- Prior art keywords
- terahertz
- optical fiber
- animal
- hole
- head fixing
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 208000024827 Alzheimer disease Diseases 0.000 title claims abstract description 40
- 206010039966 Senile dementia Diseases 0.000 title claims abstract description 16
- 230000013016 learning Effects 0.000 title claims abstract description 15
- 230000015654 memory Effects 0.000 title claims abstract description 14
- 238000000034 method Methods 0.000 title description 18
- 241001465754 Metazoa Species 0.000 claims abstract description 46
- 239000013307 optical fiber Substances 0.000 claims abstract description 36
- 238000010171 animal model Methods 0.000 claims abstract description 22
- 230000033001 locomotion Effects 0.000 claims abstract description 11
- 210000003625 skull Anatomy 0.000 claims abstract description 8
- 230000009194 climbing Effects 0.000 claims description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 6
- 238000002347 injection Methods 0.000 claims description 5
- 239000007924 injection Substances 0.000 claims description 5
- 241000699670 Mus sp. Species 0.000 description 24
- 210000003128 head Anatomy 0.000 description 22
- 230000005855 radiation Effects 0.000 description 13
- 206010012289 Dementia Diseases 0.000 description 12
- 238000010175 APPswe/PSEN1dE9 Methods 0.000 description 10
- 241000699666 Mus <mouse, genus> Species 0.000 description 9
- 238000002474 experimental method Methods 0.000 description 9
- 238000011160 research Methods 0.000 description 8
- 238000005516 engineering process Methods 0.000 description 7
- 239000003814 drug Substances 0.000 description 6
- 230000000971 hippocampal effect Effects 0.000 description 6
- 229940079593 drug Drugs 0.000 description 5
- 230000000694 effects Effects 0.000 description 5
- 230000008569 process Effects 0.000 description 5
- 208000037259 Amyloid Plaque Diseases 0.000 description 4
- 230000001149 cognitive effect Effects 0.000 description 4
- 230000008021 deposition Effects 0.000 description 4
- 210000001320 hippocampus Anatomy 0.000 description 4
- 230000006872 improvement Effects 0.000 description 4
- 238000007619 statistical method Methods 0.000 description 4
- 238000002965 ELISA Methods 0.000 description 3
- 230000006399 behavior Effects 0.000 description 3
- 230000003542 behavioural effect Effects 0.000 description 3
- 230000036760 body temperature Effects 0.000 description 3
- 210000004556 brain Anatomy 0.000 description 3
- 230000003920 cognitive function Effects 0.000 description 3
- 230000001054 cortical effect Effects 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000000638 stimulation Effects 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 108090001005 Interleukin-6 Proteins 0.000 description 2
- 208000036110 Neuroinflammatory disease Diseases 0.000 description 2
- 238000000692 Student's t-test Methods 0.000 description 2
- 230000006933 amyloid-beta aggregation Effects 0.000 description 2
- 210000005013 brain tissue Anatomy 0.000 description 2
- 210000003169 central nervous system Anatomy 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 238000010166 immunofluorescence Methods 0.000 description 2
- 230000001678 irradiating effect Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 210000005036 nerve Anatomy 0.000 description 2
- 230000003959 neuroinflammation Effects 0.000 description 2
- 230000007170 pathology Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 230000003956 synaptic plasticity Effects 0.000 description 2
- MZOFCQQQCNRIBI-VMXHOPILSA-N (3s)-4-[[(2s)-1-[[(2s)-1-[[(1s)-1-carboxy-2-hydroxyethyl]amino]-4-methyl-1-oxopentan-2-yl]amino]-5-(diaminomethylideneamino)-1-oxopentan-2-yl]amino]-3-[[2-[[(2s)-2,6-diaminohexanoyl]amino]acetyl]amino]-4-oxobutanoic acid Chemical compound OC[C@@H](C(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCN=C(N)N)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@@H](N)CCCCN MZOFCQQQCNRIBI-VMXHOPILSA-N 0.000 description 1
- 206010001497 Agitation Diseases 0.000 description 1
- 229940122041 Cholinesterase inhibitor Drugs 0.000 description 1
- 241000699660 Mus musculus Species 0.000 description 1
- 102000015336 Nerve Growth Factor Human genes 0.000 description 1
- 108010025020 Nerve Growth Factor Proteins 0.000 description 1
- 208000037273 Pathologic Processes Diseases 0.000 description 1
- 206010034719 Personality change Diseases 0.000 description 1
- 238000011869 Shapiro-Wilk test Methods 0.000 description 1
- 108060008682 Tumor Necrosis Factor Proteins 0.000 description 1
- 102000000852 Tumor Necrosis Factor-alpha Human genes 0.000 description 1
- 201000004810 Vascular dementia Diseases 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 108010064539 amyloid beta-protein (1-42) Proteins 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004958 brain cell Anatomy 0.000 description 1
- 210000003710 cerebral cortex Anatomy 0.000 description 1
- 239000000544 cholinesterase inhibitor Substances 0.000 description 1
- 208000035850 clinical syndrome Diseases 0.000 description 1
- 208000010877 cognitive disease Diseases 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 238000003125 immunofluorescent labeling Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000003137 locomotive effect Effects 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000010172 mouse model Methods 0.000 description 1
- 229940053128 nerve growth factor Drugs 0.000 description 1
- 210000000653 nervous system Anatomy 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 208000015122 neurodegenerative disease Diseases 0.000 description 1
- 230000002314 neuroinflammatory effect Effects 0.000 description 1
- 239000002858 neurotransmitter agent Substances 0.000 description 1
- 238000001422 normality test Methods 0.000 description 1
- 230000008506 pathogenesis Effects 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 230000009054 pathological process Effects 0.000 description 1
- 238000006303 photolysis reaction Methods 0.000 description 1
- 230000015843 photosynthesis, light reaction Effects 0.000 description 1
- 230000004962 physiological condition Effects 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 210000004761 scalp Anatomy 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 230000009261 transgenic effect Effects 0.000 description 1
- 238000011830 transgenic mouse model Methods 0.000 description 1
- 238000007492 two-way ANOVA Methods 0.000 description 1
- 230000003936 working memory Effects 0.000 description 1
Images
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/02—Breeding vertebrates
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2267/00—Animals characterised by purpose
- A01K2267/03—Animal model, e.g. for test or diseases
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61D—VETERINARY INSTRUMENTS, IMPLEMENTS, TOOLS, OR METHODS
- A61D3/00—Appliances for supporting or fettering animals for operative purposes
- A61D2003/003—Appliances for supporting or fettering animals for operative purposes with head or neck restraining means
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Veterinary Medicine (AREA)
- Biodiversity & Conservation Biology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Radiation-Therapy Devices (AREA)
Abstract
The invention belongs to the field of terahertz wave treatment, and relates to a device for preventing and treating senile dementia and improving learning memory based on terahertz wave intervention, which comprises a terahertz irradiation platform, wherein the terahertz irradiation platform comprises a terahertz generator, a terahertz waveguide, a TPX lens, an optical fiber adjusting frame, a conducting optical fiber, an animal head fixing clamp and an animal motion system; the terahertz generator is used for generating high-power stable terahertz waves; the terahertz waveguide is used for collecting terahertz waves and amplifying power, the terahertz waves are polymerized through the TPX lens, connected with the optical fiber through the optical fiber adjusting frame and transmitted to the experimental animal head fixing clamp through the conducting optical fiber; the animal head fixing clamp comprises a fixing arm, a fixing plectrum and a central hole, wherein the central hole is connected with a conducting optical fiber, is of a cylindrical structure and is externally provided with scales and used for calculating the distance between the terahertz optical fiber and a skull; the fixed shifting piece is provided with a screw hole, and the screw hole is fixed on the skull of the animal through the fixed shifting piece.
Description
Technical Field
The invention belongs to the field of terahertz wave treatment, and relates to a method for preventing and treating senile dementia and improving learning and memory based on terahertz wave intervention.
Background
China is entering the old population, and the incidence of degenerative diseases of the nervous system will rise sharply in a series of changes. The onset of Alzheimer's Disease (AD) is the first leading problem. Senile dementia is a clinical syndrome mainly caused by hypofunction and behavior and personality change, and the hypofunction is the most prominent symptom. Pathologically, senile dementia is characterized by senile plaques and entanglement of neuronal fibers. Senile dementia not only seriously affects the life quality of patients, but also brings heavy burden to the society and families, has attracted high attention of governments of various countries, and all countries in the world fund a huge amount to research the medicines for preventing and treating the senile dementia.
The existing drugs for treating AD, such as cholinesterase inhibitor, nerve growth factor, brain cell activator and the like, mainly aim at improving the clinical symptoms of patients, but not aim at the pathogenesis of AD and block the pathological process of AD, so the development of the disease cannot be effectively prevented. Moreover, the long-term application of the drugs has serious inevitable side effects or difficult sources, and cannot be popularized in clinical application, so that the drugs are difficult to develop into ideal drugs for treating AD.
Terahertz waves belong to the category of electromagnetic waves, and electromagnetic stimulation technology has been widely used in psychiatry, neurology and other clinical specialties since the 20 th century, 80 s, as a non-invasive intervention, non-invasive method of delivering electromagnetic stimulation to the brain through the intact scalp. The existing research shows that the brain electromagnetic stimulation can generate long-term influence on neurotransmitter and synaptic plasticity by changing the excitability of cerebral cortex, and can improve the cognitive function and the synaptic plasticity. At present, cognitive dysfunction diseases including Alzheimer's disease, vascular dementia and the like still lack effective treatment means, so that the related problems need to be solved by THz exposure experimental animals.
Disclosure of Invention
The invention aims to provide a method for preventing and treating senile dementia and improving learning and memory based on terahertz wave intervention, which provides a novel noninvasive treatment means for preventing and treating senile dementia and improving learning and memory by low-frequency terahertz microwave irradiation.
The Terahertz (Terahertz or THz) wave generally refers to an electromagnetic wave with the frequency of 0.1THz-10THz, the photon energy of the electromagnetic wave is about 0.3meV, the photolysis of biological molecules is not caused, and the biological safety is very good. Historically, due to the lack of efficient terahertz sources and detection devices, the research on the band has been relatively rare, becoming a "terahertz gap". In recent years, with the breakthrough of ultrafast laser technology and vacuum electronics, terahertz technology is rapidly developed and becomes a research hotspot. Relevant theory and experimental research show that the rotation energy level of the biological molecules is between 0.001 and 0.3cm -1 The internal rotation energy level is 0.3-11 cm -1 The low-frequency vibration energy level is 11-250 cm -1 Are all in terahertzA band. Therefore, terahertz waves become an ideal tool for exploring biomolecules and life sciences, and have been widely used in the research of life sciences.
The technical scheme of the invention is as follows:
a method for preventing and treating senile dementia and improving learning and memory based on terahertz wave intervention comprises the following steps:
the first step is as follows: fixing the dementia model animal on a radiation platform, and fixing the dementia model animal by an animal head fixing clamp to adjust the comfort degree of the animal;
the second step: the craniocerebral stereotaxic apparatus is used to select the head irradiation part of the experimental animal, namely the Baihui acupoint which is the central nervous system for regulating the nerve function.
The third step: the radiation power of a terahertz generator using a terahertz irradiation platform is adjusted to be 10mW/cm 2 Irradiating the experimental animal;
the fourth step: in the whole radiation process, the temperature of the experimental animal is monitored by an infrared temperature tester, and the fluctuation of the body temperature is controlled to be +/-0.1 ℃; meanwhile, the radiation process is dynamically monitored through a terahertz power tester.
The terahertz irradiation platform comprises a terahertz generator, a terahertz waveguide, a TPX lens, an optical fiber adjusting frame, a conducting optical fiber, an animal head fixing clamp and an animal motion system;
the terahertz generator is used for generating high-power stable terahertz waves.
The terahertz waveguide is used for collecting terahertz waves and expanding power, the terahertz waves are polymerized through the TPX lens, connected with optical fibers through the optical fiber adjusting frame and transmitted to the fixing clamp for the head of the experimental animal through the conducting optical fibers.
The animal head fixing clamp comprises a fixing arm, a fixing shifting piece and a central hole, wherein the central hole is connected with a conducting optical fiber, is of a cylindrical structure and is externally provided with scales and used for calculating the distance between the terahertz optical fiber and the skull. The fixed shifting piece is provided with a screw hole, and the screw is fixed on the animal skull through the screw hole of the fixed shifting piece.
The diameter of the center hole of the animal head fixing shifting piece is 3mm.
The diameter of the screw hole of the animal head fixing plectrum is 1.1mm.
The animal motion system comprises a fixed rod, a climbing ball and a ball water injection hole, and the weight of the ball can be adjusted according to the month ages of different animals, so that the animal motion system is suitable for different experimental animal requirements.
The small animal THz irradiation platform is provided with a high-power terahertz wave generator; the animal fixing clamp is provided with the center hole connected with the terahertz optical fiber, when the head of an experimental animal needs to be subjected to a radiation experiment, the head of the experimental animal can be fixed on the clamp, a radiation part is exposed, the terahertz optical fiber can accurately act on a specific part of the head, and other parts cannot be exposed under terahertz waves, so that terahertz radiation efficiency is improved. In addition, the influence of different distance exposure on organisms can be evaluated by controlling the distance between an experimental animal and the end of the terahertz optical fiber.
The invention has the beneficial effects that:
the invention discloses a method for preventing and treating senile dementia and improving learning and memory based on terahertz wave intervention, which comprises a three-dimensional positioning technology of acupoint irradiation of an experimental animal, a dynamic monitoring technology of terahertz irradiation power, body temperature monitoring in the irradiation process of the experimental animal, establishment description of a THz irradiation platform and an evaluation system of experimental effect after THz irradiation is received. The method can realize THz non-invasive physical intervention treatment, maximally simulate the physiological condition state of the experimental animal, and reduce the influence of human factors on the experimental result. The treatment method overcomes the defects of the traditional medicine method, and the embodiment also shows that the method can really realize the improvement of the cognitive function, can be used for laying a cushion for the subsequent relevant mechanism of treating the senile dementia by the terahertz, and provides a thought for the research of treating the senile dementia.
Drawings
FIG. 1 is a schematic diagram of an experimental platform;
FIG. 2 is a schematic view of an animal head retaining clip;
FIG. 3 is a schematic view of an animal locomotion system;
FIG. 4 is a schematic representation of the cognitive behavioral changes following THz stem acceptance in APP/PS1 AD mice;
FIG. 5 is a graph showing the levels of hippocampal and cortical A β deposition following THz intervention in APP/PS1 AD mice.
In the figure: 1 a terahertz generator; 2, terahertz waveguide; 3 TPX lens; 4, an optical fiber adjusting frame; 5 a conducting optical fiber; 6, animal head fixing clips; 67 a fixed arm; 68 fixing the shifting sheet; 69 a central aperture; 70 screw holes; 7 an animal locomotor system; 77 fixing the rod; 78 climbing a ball; 79 ball water injection holes; 8, an infrared temperature tester; 9 terahertz power tester.
Detailed Description
The invention is further described below with reference to the accompanying drawings.
Example 1:
a method for preventing and treating senile dementia and improving learning and memory based on terahertz wave intervention comprises the following steps:
the first step is as follows: fixing the dementia model animal on a radiation platform, and fixing the dementia model animal by an animal head fixing clamp 6 to adjust the comfort degree of the animal;
the second step is that: the craniocerebral stereotaxic apparatus is used to select the head irradiation part of the experimental animal, namely the Baihui acupoint which is the central nervous system for regulating the nerve function.
The third step: the terahertz generator 1 of the terahertz irradiation platform is used for adjusting the radiation power to be 10mW/cm 2 Irradiating the experimental animal;
the fourth step: in the whole radiation process, the temperature of the experimental animal is monitored by an infrared temperature tester 8, and the fluctuation of the body temperature is controlled to be +/-0.1 ℃; meanwhile, the radiation process is dynamically monitored by the terahertz power tester 9.
The terahertz irradiation platform comprises a terahertz generator 1, a terahertz waveguide 2, a TPX lens 3, an optical fiber adjusting frame 4, a conducting optical fiber 5, an animal head fixing clamp 6 and an animal motion system 7;
the terahertz generator 1 is used for generating high-power stable terahertz waves.
The terahertz waveguide 2 is used for collecting terahertz waves and expanding power, the terahertz waves are polymerized through the TPX lens 3, connected with optical fibers through the optical fiber adjusting frame 4 and transmitted to the experimental animal head fixing clamp 6 through the conducting optical fibers 5.
The animal head fixing clamp 6 comprises a fixing arm 67, a fixing shifting piece 68 and a central hole 69, wherein the central hole 69 is connected with the conducting optical fiber 5, the hole is of a cylindrical structure and is provided with scales, and the hole is used for calculating the distance between the terahertz optical fiber and the skull. The screw is fixed on the skull of the animal through the screw hole 70 of the fixing plectrum 68. The diameter of the central hole 69 of the animal head fixing shifting piece 68 is 3mm. The diameter of the screw hole 70 of the animal head fixing plectrum 68 is 1.1mm.
The animal movement system 7 comprises a fixing rod 77, a climbing ball 78 and a ball water injection hole 79, the ball water injection hole 79 is positioned on the climbing ball 78, the climbing ball 78 is movable, the weight of the ball can be adjusted according to the month ages of different animals, and the requirements of different experimental animals are met.
Example 2:
the animals were tested as follows:
1. materials and methods
1.1 Experimental animals
SPF-grade double transgenic APP/PS1 mice (5 months old) and same month old WT mice were selected. All mice were housed in plastic cages in temperature controlled (18-25 ℃) colony chambers with a 12 hour light/12 hour dark cycle, and all experiments were performed during the light cycle. Food and water are available ad libitum. The genotype of the mice was confirmed by polymerase chain reaction using DNA from tail tissue.
1.2 animal grouping and intervention modalities
APP/PS1 transgenic mice were randomly divided into two groups: AD-Sham group and AD-THz group. The mouse is awake, and the irradiation part is positioned by fixing a cap on the head. The terahertz optical fiber is connected through a circular head cap, so that the terahertz optical fiber is fixed on the opening of the conical tube, and the irradiation distance can be adjusted. AD-THZ group mice received 1 time THz treatment per day with effective irradiation time of 20min. The AD-Sham group terahertz optical fiber head cap is fixed, but the THz light source is not turned on.
1.3 study of learning and memory behaviourology
Maze experiment, new object discernment experiment, commentaries on classics stick experiment: and detecting and evaluating the learning, memory, movement and other abilities of the mice.
1.4 histopathological Studies
After the behaviourology is finished, taking materials from brain tissues, embedding, freezing, slicing, sealing, incubating antibodies, taking a picture by a microscope, and observing the change condition of senile plaques by applying an immunofluorescence staining technology.
Table 1 immunofluorescence Using antibody names
1.5 ELISA for detection of Abeta levels
The concentration of a β 42 (us Invitrogen, KHB 354) in hippocampal brain extracts was measured by ELISA according to the manufacturer's instructions.
1.6 neuroinflammatory effects
The concentration of IL-6, TNF-. Alpha.in brain tissue extracts (RayBio, ELM-IL6, ELM-TNF-. Alpha.in the USA) was measured by ELISA according to the manufacturer's instructions.
1.7 statistical analysis: statistical analysis was performed using GraphPad Prism 7 software. The data normality test was performed by Shapiro-Wilk test, all data are expressed as mean. + -. Standard error, and statistical analysis was performed by Student's t-test, two-way ANOVA analysis (multiple comparisons were performed with Tukey's multiple complexes test). P <0.05 was considered statistically significant.
2. Results
2.1 behavioural recording: to evaluate the effect of THZ radiation on mouse cognitive function, we examined cognitive behavior of dementia model mice after terahertz radiation. The result shows that the new object identification experiment result shows that: after the mice are irradiated by the terahertz waves for 3 months, the proportion of exploring new objects of the mice of the dementia model in the terahertz group is obviously improved (p is less than 0.05) compared with the mice of the dementia model in the non-terahertz group, and the improvement of the recognition memory of the mice of the dementia model after the terahertz wave irradiation is prompted (as shown in figure 4A). The Y-maze experiment result shows that: the space correct exploration ratio of the terahertz group dementia model mouse is remarkably improved (p is less than 0.05) compared with that of a non-terahertz group dementia model mouse, and the improvement of the working memory of the AD mouse after terahertz irradiation is prompted (as shown in figure 4B). The results of the RotaRod experiment show that the incubation period when the AD mice fall and the maximum rotating speed when the AD mice fall have no obvious difference among all groups (as shown in figures 4C and D), and the change of the cognitive behaviours of the AD mice is not related to the motor ability.
FIG. 4 is a graph of cognitive behavioral changes in APP/PS1 AD mice after receiving THz intervention. AD mouse NORT test results (A), Y-maze test results (B); rotaRod experiment AD mice maximum speed of rotation (C, D) when dropped. Data are expressed by mean +/-standard error, and 10-14 mice in each group; data were analyzed by Student's ttest with P <0.05, and ns was not significantly different.
2.2 A β immunofluorescence histopathology:
a beta is a main pathological marker of AD, and in order to verify the influence of THz microwave treatment on A beta pathology, the deposition levels of hippocampal and intradermal amyloid plaques of an APP/PS1 dementia model mouse are detected. As shown in fig. 2, THz group AD mice had reduced deposition of SPs in hippocampus and cortex; statistics show that the area occupied by SPs is obviously reduced in the AD-THz group mice compared with the AD-Sham group mice, and the THz irradiation is prompted to reduce the A beta pathology of the AD mice.
FIG. 5 is the levels of hippocampal and cortical A β deposition following THz intervention in APP/PS1 AD mice. (A, B) 6E10 antibody staining shows a reduction in hippocampal and cortical SPs deposition in AD mice following THz intervention; (C, D) statistical analysis shows that the occupied areas of the SPs in the hippocampus and the cortex of the AD mouse are obviously reduced compared with the control group after THz intervention. Data are expressed as mean ± standard error, 6 mice per group, data analyzed as Student's t test,. P <0.05.
2.3 A β 42 metabolic level:
table 2: hippocampus Abeta 42 level comparison of 8-month-old APP/PS1 mice
(n＝5，pg/mg)
Note: * P is less than 0.05 compared with AD-Sham
2.4 neuroinflammation levels:
table 3: hippocampus IL-6 level comparison in APP/PS1 mice at 8 months of age
(n＝7，pg/ml)
Note: * P is less than 0.05 compared with AD-Sham
Table 3: hippocampal TNF-alpha level comparison of APP/PS1 mice at 8 months of age
(n＝6，pg/ml)
Note: * Compared with AD-Sham, p is less than 0.05
The experimental results are combined, and the THz microwave irradiation has an improvement effect on the space learning capacity of the dementia model mouse, has a remarkable effect on the related diseases of space learning, and can enhance the learning and memory capacity of AD experimental animals, reduce the AD senile plaque deposition and improve the neuroinflammation level. Therefore, the technology has huge potential of potential application value and is worthy of popularization and deep research.
The foregoing examples are provided for illustration and description of the invention only and are not intended to limit the invention to the scope of the described examples. Furthermore, it will be understood by those skilled in the art that the present invention is not limited to the embodiments described above, and that many variations and modifications may be made in accordance with the teachings of the present invention, which variations and modifications are within the scope of the present invention as claimed.
Claims (1)
1. The device for preventing and treating senile dementia and improving learning and memory based on terahertz wave intervention comprises a terahertz irradiation platform and is characterized in that the terahertz irradiation platform comprises a terahertz generator (1), a terahertz waveguide (2), a TPX lens (3), an optical fiber adjusting frame (4), a conducting optical fiber (5), an animal head fixing clamp (6) and an animal motion system (7);
the terahertz generator (1) is used for generating high-power stable terahertz waves;
the terahertz waveguide (2) is used for collecting terahertz waves and expanding power, the terahertz waves are polymerized through the TPX lens (3), connected with optical fibers through the optical fiber adjusting frame (4) and transmitted to the experimental animal head fixing clamp (6) through the conducting optical fibers (5);
the animal head fixing clamp (6) comprises a fixing arm (67), a fixing shifting piece (68) and a central hole (69), wherein the central hole (69) is connected with the conducting optical fiber (5), the hole is of a cylindrical structure, scales are arranged outside the hole, and the hole is used for calculating the distance between the terahertz optical fiber and a skull;
the animal movement system (7) comprises a fixing rod (77), a climbing ball (78) and a ball water injection hole (79), and the weight of the ball can be adjusted according to the month ages of different animals, so that the animal movement system is suitable for the requirements of different experimental animals;
the diameter of a central hole (69) of the animal head fixing shifting sheet (68) is 3mm;
the diameter of a screw hole (70) of the animal head fixing plectrum (68) is 1.1mm;
the fixed shifting piece (68) is provided with a screw hole (70), and the screw is fixed on the animal skull through the screw hole (70) of the fixed shifting piece (68).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111533230.0A CN113973778B (en) | 2021-12-15 | 2021-12-15 | Method for preventing and treating senile dementia and improving learning and memory based on terahertz wave intervention |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202111533230.0A CN113973778B (en) | 2021-12-15 | 2021-12-15 | Method for preventing and treating senile dementia and improving learning and memory based on terahertz wave intervention |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN113973778A CN113973778A (en) | 2022-01-28 |
| CN113973778B true CN113973778B (en) | 2023-01-17 |
Family
ID=79733928
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202111533230.0A Active CN113973778B (en) | 2021-12-15 | 2021-12-15 | Method for preventing and treating senile dementia and improving learning and memory based on terahertz wave intervention |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113973778B (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105963355A (en) * | 2016-06-14 | 2016-09-28 | 云南瑞酚生物科技有限公司 | Medicine for preventing and treating senile dementia |
| CN113648542A (en) * | 2021-08-27 | 2021-11-16 | 青岛大学 | Rehabilitation treatment device and comprehensive treatment device for intracranial nervous system diseases |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19748720A1 (en) * | 1996-12-27 | 1998-07-02 | Themys Gmbh | Therapeutic treatment device using electromagnetic waves |
| KR20060119325A (en) * | 2005-05-19 | 2006-11-24 | 배병근 | Device and method of protecting from earth energies |
| CN201098217Y (en) * | 2007-11-30 | 2008-08-13 | 吴伋用 | Heat accumulation moxibustion therapeutic device for zoopery |
| US8933295B2 (en) * | 2008-06-20 | 2015-01-13 | Janssen Biotech, Inc. | Identifying agents to treat alzheimer's disease-related decreased sorting behavior by administration to a triple transgenic mouse expressing mutant forms of APP, presenilin or tau |
| CN101797267B (en) * | 2010-04-28 | 2012-03-21 | 北京大学第一医院 | Medical composite for preventing and curing Alzheimer's disease |
| CN102742516B (en) * | 2012-07-21 | 2014-06-11 | 深圳市华测检测技术股份有限公司 | System and method for training primates to play games |
| CN110141750A (en) * | 2019-04-26 | 2019-08-20 | 上海金光柔生物科技有限公司 | A kind of multipurpose wide spectrum therapeutic equipment |
| CN110333310B (en) * | 2019-08-16 | 2022-02-11 | 大连医科大学附属第一医院 | Group of biomarkers for diagnosing AD in a subject or determining the risk of developing AD in a subject and uses thereof |
| CN113384375B (en) * | 2021-06-18 | 2024-04-30 | 中国人民解放军陆军军医大学第二附属医院 | Experimental device for tail fixation of tail intravenous injection of mice |
-
2021
- 2021-12-15 CN CN202111533230.0A patent/CN113973778B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105963355A (en) * | 2016-06-14 | 2016-09-28 | 云南瑞酚生物科技有限公司 | Medicine for preventing and treating senile dementia |
| CN113648542A (en) * | 2021-08-27 | 2021-11-16 | 青岛大学 | Rehabilitation treatment device and comprehensive treatment device for intracranial nervous system diseases |
Non-Patent Citations (1)
| Title |
|---|
| 阿尔茨海默病的预后与预防;陈雪;《中国实用乡村医生杂志》;20200531;全文 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN113973778A (en) | 2022-01-28 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Gelinas et al. | Interictal epileptiform discharges induce hippocampal–cortical coupling in temporal lobe epilepsy | |
| EP3435860B1 (en) | Methods for determining cognitive impairment using magnetoencephalography data | |
| George et al. | Mood improvement following daily left prefrontal repetitive transcranial magnetic stimulation in patients with depression: a placebo-controlled crossover trial | |
| Grimaldi et al. | Non-invasive cerebellar stimulation—a consensus paper | |
| Bateson et al. | Imprinting: lasting effects on uracil incorporation into chick brain | |
| Gómez et al. | Cerebellum lesion impairs eyeblink-like classical conditioning in goldfish | |
| CA3135689A1 (en) | Methods and magnetic imaging devices to inventory human brain cortical function | |
| Kanda et al. | Sleep as a biological problem: an overview of frontiers in sleep research | |
| Geman | Data processing for Parkinson's disease: Tremor, speech and gait signal analysis | |
| Riekkinen Jr et al. | Effects of quisqualic acid nucleus basalis lesioning on cortical EEG, passive avoidance and water maze performance | |
| CN109567803A (en) | Hippocampus self-control analysis method based on real-time neural feedback technology | |
| CN113973778B (en) | Method for preventing and treating senile dementia and improving learning and memory based on terahertz wave intervention | |
| Hu et al. | Effects of transcranial alternating current stimulation on motor performance and motor learning for healthy individuals: A systematic review and meta-analysis | |
| Zhao et al. | Modulation effects of low-intensity transcranial ultrasound stimulation on the neuronal firing activity and synaptic plasticity of mice | |
| CN114712735A (en) | Ultrasonic therapeutic apparatus for neurodegenerative diseases and use method thereof | |
| Tseng et al. | Region-specific effects of ultrasound on individual neurons in the awake mammalian brain | |
| Adams et al. | Electrophysiological correlates of categorical speech perception for voicing contrasts in dogs | |
| Anjum et al. | Linear Predictive Approaches Separate Field Potentials in Animal Model of Parkinson's Disease | |
| Beck et al. | 035 Activating the concept of “relaxation” during sleep using relaxation-related words | |
| CN112205977A (en) | Biofeedback treatment method for improving insomnia based on functional magnetic resonance | |
| Jiang et al. | Visuospatial properties of caudal area 7b in Macaca fascicularis | |
| Ricciardiello et al. | 033 The effect of sleep disturbance on cognition in late-life depression | |
| Riley et al. | TDCS-mediated artificial grammar training shows potential for improving attention and facilitating language learning in persons with aphasia | |
| Zhao et al. | Study on the Regulation Effect of Optogenetic Technology on LFP of the Basal Ganglia Nucleus in Rotenone‐Treated Rats | |
| Krepel et al. | Clinical and neurophysiological predictors of rTMS response in Major Depressive Disorder: Robustness and clinical relevance |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| CB03 | Change of inventor or designer information |
Inventor after: Yue Weidong Inventor after: Zhang Jun Inventor after: Li Song Inventor before: Yue Weidong Inventor before: Zhang Jun Inventor before: Li Song |
|
| CB03 | Change of inventor or designer information | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |