CN105949295A - Protein relevant to plant flowering time and encoding gene and application thereof - Google Patents

Protein relevant to plant flowering time and encoding gene and application thereof Download PDF

Info

Publication number
CN105949295A
CN105949295A CN201610561640.9A CN201610561640A CN105949295A CN 105949295 A CN105949295 A CN 105949295A CN 201610561640 A CN201610561640 A CN 201610561640A CN 105949295 A CN105949295 A CN 105949295A
Authority
CN
China
Prior art keywords
plant
gene
protein
sequence
early
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201610561640.9A
Other languages
Chinese (zh)
Other versions
CN105949295B (en
Inventor
吴存祥
王志莉
冯永君
韩天富
刘薇
韩祥东
侯文胜
孙�石
蒋炳军
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute of Crop Sciences of Chinese Academy of Agricultural Sciences
Original Assignee
Institute of Crop Sciences of Chinese Academy of Agricultural Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institute of Crop Sciences of Chinese Academy of Agricultural Sciences filed Critical Institute of Crop Sciences of Chinese Academy of Agricultural Sciences
Priority to CN201610561640.9A priority Critical patent/CN105949295B/en
Publication of CN105949295A publication Critical patent/CN105949295A/en
Application granted granted Critical
Publication of CN105949295B publication Critical patent/CN105949295B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/827Flower development or morphology, e.g. flowering promoting factor [FPF]

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Botany (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Physiology (AREA)
  • Plant Pathology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

The invention discloses protein relevant to plant flowering time and an encoding gene and application thereof. The protein is named GmNMHC5 protein and is obtained from Glycine max (L.) Merrill. The protein is a or b, wherein a is composed of an amino acid sequence as shown in sequence 1 in the sequence table, and b is derived from sequence 1, obtained by conducting substitution and/or deficiency and/or addition of one or more amino acid residues on the amino acid sequence as shown in sequence 1, and relevant to plant flowering time. The gene encoding the GmNMHC5 protein (GmNMHC5 gene) is also within the protection range of the invention. The GmNMHC5 protein and the encoding gene thereof can make plant flowering time earlier and shorten the juvenile phase. The protein relevant to plant flowering time and the encoding gene thereof have high application value in plant breeding.

Description

With the albumen of flowering of plant time correlation and encoding gene thereof and application
Technical field
Biological technical field of the present invention, particularly relate to a kind of albumen with flowering of plant time correlation and encoding gene thereof with Application.
Background technology
Semen sojae atricolor is the most of paramount importance oil crop and high protein cereal crops, in human lives and international agriculture Trade occupies very important status, plays not in terms of culture fertility, the improvement farming such as soil, crop rotation Alternative effect.Blooming is the plant significant process that turned to reproductive growth by nourishing and growing, is one of plant growing The topmost stage.As typical short day crop, the process of blooming of Semen sojae atricolor is by the strict regulation and control of photoperiod.Greatly The bloom clone of related gene and functional study of bean, to instructing Semen sojae atricolor to grow normally, improves crop yield and has important Meaning.
Research ontogenetic to model plant shows, the process of blooming of plant is mainly regulated and controled by four approach: the photoperiod Approach (photoperiod pathway), vernalization approach (vernalization pathway), spontaneous approach (autonomous pathway) and gibberellin pathway (GA pathway), the regulated and control network pair that these four approach are constituted The expression of the Blossoms Correlation Gene in Plants strictly controls.Correlational study shows, long day plant (such as arabidopsis) and short day plant The molecular pathways that thing (such as Oryza sativa L.) is bloomed is conservative on evolving.
Summary of the invention
It is an object of the invention to provide a kind of albumen with flowering of plant time correlation and encoding gene thereof and application.
Present protein, named GmNMHC5 albumen, available from Semen sojae atricolor (Glycine max (L.) Merrill), It is following (a) or (b):
A protein that () is made up of the aminoacid sequence shown in sequence in sequence table 1;
B the aminoacid sequence of sequence 1 through replacement and/or the disappearance of one or several amino acid residue and/or is added by () Add and with the protein derived by sequence 1 of flowering of plant time correlation.
In order to make the protein in (a) be easy to purification, can in by sequence table the aminoacid sequence shown in sequence 1 The amino terminal of the protein of row composition or carboxyl terminal connect upper label as shown in table 1.
The sequence of table 1 label
Protein in above-mentioned (b) can synthetic, it is possible to first synthesize its encoding gene, then carries out biological expression and obtain. The encoding gene of the protein in above-mentioned (b) can be by lacking one in the DNA sequence shown in sequence in sequence table 2 Or the codon of several amino acid residue, and/or carry out the missense mutation of one or several base pair, and/or its 5 ' End and/or 3 ' ends connect the coded sequence of the label shown in table 1 and obtain.
The gene (GmNMHC5 gene) encoding described GmNMHC5 albumen falls within protection scope of the present invention.
Described GmNMHC5 gene following 1) or 2) or 3) DNA molecular:
1) coding region DNA molecular as shown in sequence 2 in sequence table;
2) under strict conditions with 1) the DNA sequence hybridization that limits and the DNA of coded plant flowering time associated protein Molecule;
3) with 1) or 2) DNA sequence that limits at least has 70%, at least has 75%, at least has 80%, extremely Have 85% less, at least have 90%, at least have 95%, at least have 96%, at least have 97%, at least have 98% or at least there is more than 99% homology and the DNA molecular of coded plant flowering time associated protein.
Above-mentioned stringent condition can be 6 × SSC, 0.5%SDS solution in, at 65 DEG C hybridize, then with 2 × SSC, 0.1%SDS and 1 × SSC, 0.1%SDS respectively wash film once.
Recombinant expression carrier, expression cassette, transgenic cell line or recombinant bacterium containing described GmNMHC5 gene belong to Protection scope of the present invention.
Available existing plant expression vector construction contains the recombinant expression carrier of GmNMHC5 gene.Described plant is expressed Carrier includes double base agrobacterium vector and can be used for the carrier etc. of plant micropellet bombardment.Described plant expression vector also can wrap 3 ' the end untranslated regions containing exogenous gene, i.e. comprise polyadenylation signals and any other participates in mRNA processing or base Because of the DNA fragmentation expressed.The bootable polyadenylic acid of described polyadenylation signals joins 3 ' ends of mRNA precursor.Make During recombinant expression carrier gene constructed with GmNMHC5, can be plus any enhancement mode before its transcription initiation nucleotide Promoter or constitutive promoter, they can be used alone or be used in combination with other plant promoter;Additionally, make During recombinant expression carrier gene constructed with GmNMHC5, it be also possible to use enhancer, including translational enhancer or transcribe enhancing Son, but must be identical with the reading frame of coded sequence, to ensure the correct translation of whole sequence.Described translation controls letter Number and the source of start codon be widely, can be natural, it is also possible to be synthesis.Translation initiation region can Since from transcription initiation region or structural gene.For the ease of transgenic plant cells or plant being identified and screening, Plant expression vector used can be processed, the enzyme of color change can be produced as added the coding can expressed in plant Or the gene of luminophor, the antibiotic marker thing with resistance or anti-chemical reagent marker gene etc..From turning base Because of the security consideration of plant, any selected marker can be not added with, directly with character screening transformed plant.
In described expression cassette, the expression of GmNMHC5 gene can be started by constitutive promoter.Described constitutive promoter Concretely 35S promoter.
Described recombinant expression carrier concretely inserts GmNMHC5 gene in the multiple clone site of carrier pGFPGUSplus The recombiant plasmid obtained.Described recombinant expression carrier is concretely by Xba I and Sac I enzyme of carrier pGFPGUSplus Cut the small fragment between site and be substituted by the recombiant plasmid that GmNMHC5 gene obtains.
The present invention also protects the application of GmNMHC5 albumen, at least one in following (c1) to (c4):
(c1) florescence of plant is regulated and controled;
(c2) advance flowering period of plant is promoted;
(c3) flowering time of plant is regulated and controled;
(c4) flowering time of plant is promoted in advance.
Described plant is monocotyledon or dicotyledon.
Described dicotyledon can be arabidopsis, such as Columbia ecotype arabidopsis.
The present invention also protects a kind of method cultivating transgenic plant, is by GmNMHC5 channel genes purpose plant, To transgenic plant;At least one phenotype in described transgenic plant satisfied following (d1) to (d4):
(d1) bloom early than described purpose plant;
(d2) florescence is early than described purpose plant;
(d3) vegetative growth phase is shorter than described purpose plant;
(d4) generative growth phase is entered early than described purpose plant.
GmNMHC5 gene specifically can import described purpose plant by described recombinant expression carrier.In described method, institute Stating recombinant expression carrier can be by using Ti-plasmids, Ri plasmid, plant viral vector, directly delivered DNA, micro- Injection, conductance, the conventional biology methods such as agriculture bacillus mediated convert plant cell or tissue, and the plant group that will convert Knit and cultivate into plant.
Described purpose plant is monocotyledon or dicotyledon.
Described dicotyledon can be arabidopsis, such as Columbia ecotype arabidopsis.
The present invention also protects a kind of plant breeding method, comprises the steps (e1) and/or (e1):
(e1) by increasing the activity of GmNMHC5 albumen in purpose plant, it is thus achieved that there is the plant of following character: open Flower is shorter than described purpose early than described purpose plant and/or florescence early than described purpose plant and/or vegetative growth phase and plants Thing and/or entrance generative growth phase are early than described purpose plant;
(e2) by promoting the expression of GmNMHC5 gene in purpose plant, it is thus achieved that there is the plant of following character: open Flower is shorter than described purpose early than described purpose plant and/or florescence early than described purpose plant and/or vegetative growth phase and plants Thing and/or entrance generative growth phase are early than described purpose plant.
Described purpose plant is monocotyledon or dicotyledon.
Described dicotyledon can be arabidopsis, such as Columbia ecotype arabidopsis.
The implementation " promoting the expression of GmNMHC5 gene in purpose plant " can be as follows (f1) or (f2) or (f3):
(f1) by GmNMHC5 channel genes purpose plant;
(f2) strong promoter and/or enhancer are introduced;
(f3) other common methods in this area.
The present invention also protects recombinant expression carrier or expression described in GmNMHC5 albumen, GmNMHC5 gene or any of the above Box application in plant breeding.The purpose of described plant breeding is to cultivate the plant of advance flowering period.Described plant is single Cotyledon plant or dicotyledon.Described dicotyledon can be arabidopsis, such as Columbia ecotype arabidopsis.
The present invention is found that GmNMHC5 albumen and encoding gene thereof from Semen sojae atricolor, is conducted into the plant that sets out, and is turned Gene plant, the flowering time of this transgenic plant shifts to an earlier date than Wild plant.Therefore, GmNMHC5 albumen and coding thereof Gene has the effect promoting flowering of plant time advance.The present invention has great using value for plant breeding.
Accompanying drawing explanation
Fig. 1 is V3 phase and the R3 phase GmNMHC5 gene expression at different tissues.
Fig. 2 is the plant photo in character identification.
Fig. 3 is the flowering time in character identification and lotus throne number of blade when blooming.
Detailed description of the invention
Below example facilitates a better understanding of the present invention, but does not limit the present invention.Experiment in following embodiment Method, if no special instructions, is conventional method.Test material used in following embodiment, if no special instructions, It is and is commercially available from routine biochemistry reagent shop.Quantitative test in following example, is respectively provided with three times and repeats in fact Test, results averaged.
Carrier pGFPGUSplus: list of references: Claudia E.Vickers.pGFPGUSPlus, a new binary vector for gene expression studies and optimising transformation systems in Plants.Biotechnol Lett, 29 phases in 2007.
Agrobacterium tumefaciems GV3101: Tian Gen biochemical technology company limited.
Columbia ecotype wildtype Arabidopsis thaliana (Col-0): south intended by list of references: Guo Xiao beautiful .Columbia type Mustard callus culture is studied. Henan Agricultural Sciences, 01 phase in 2009.
Embodiment 1, GmNMHC5 albumen and the acquisition of encoding gene thereof
Verified by a large amount of sequence analysis, comparison, reading frame identification and preliminary experiment, from Semen sojae atricolor, find a new albumen, By its named GmNMHC5 albumen.
Shown in the sequence 1 of GmNMHC5 albumen such as sequence table (being made up of 241 amino acid residues).
It is GmNMHC5 gene by the unnamed gene of coding GmNMHC5 albumen, the sequence of its open reading frame such as sequence table Shown in 2 (726bp).
Embodiment 2, the express spectra of GmNMHC5 albumen
Selecting Zi Gong winter bean (photoperiod-sensitive kind) is experiment material, and short-day sunshine treatment is chosen representative Vegetative growth phase (V3 phase) and the blade of generative growth phase (R3 phase), (stem apex of V3 phase is in the R3 phase for stem apex Be changed into pod), root and root nodule be material.
Extracting the total serum IgE of each material respectively, reverse transcription is cDNA.Application Real-time RT-PCR method, with GmActin is internal standard gene, detects GmNMHC5 gene tissue specific expression situation in Semen sojae atricolor body.
Tissue expression analysis result is as shown in Figure 1.Vegetative growth phase, GmNMHC5 gene is mainly in root and root nodule Expressing, the especially expression in root is the highest.Generative growth phase, GmNMHC5 gene also has certain in soybean pod Express.Result shows, GmNMHC5 gene is not only relevant with root system development, and grows have with reproductive growth and flower pod Close.
Embodiment 3, the acquisition of transgenic plant and qualification
One, the structure of recombiant plasmid
1, the double chain DNA molecule shown in sequence 2 of composition sequence table.
2, with step 1 synthesis double chain DNA molecule as template, use F1 and R1 composition primer to carrying out PCR Amplification, obtains pcr amplification product.
F1:5 '-TCTAGAATGGGGAGAGGTAAGATTGCG-3’;
R1:5 '-GAGCTCCTAATGCAGCTGCAATCCGAGTTT-3’。
3, take carrier pGFPGUSplus, with restricted enzyme Xba I and Sac I double digestion, reclaim about 13000bp Carrier framework.
4, take the pcr amplification product that step 2 obtains, with restricted enzyme Xba I and Sac I double digestion, reclaim Digestion products.
5, the carrier framework of step 3 and the digestion products of step 4 are connected, obtain recombiant plasmid pGUS-GmNMHC5.
According to sequencing result, recombiant plasmid pGUS-GmNMHC5 is carried out structure and is described as follows: by carrier pGFPGUSplus Xba I and Sac I restriction enzyme site between small fragment replace for the double chain DNA molecule shown in the sequence 2 of sequence table. In recombiant plasmid pGUS-GmNMHC5,35S promoter start the expression of GmNMHC5 gene, by GUS as the positive The selection markers of transformation event.
Two, the acquisition of transgenic plant
1, recombiant plasmid pGUS-GmNMHC5 is imported Agrobacterium tumefaciems GV3101, obtain recombinational agrobacterium.
2, take the recombinational agrobacterium that step 1 obtains, suspend with the aqueous sucrose solution of 5g/100ml, obtain OD600nmValue Being the bacterium solution of 0.5, last every 100ml bacterium solution adds 50 μ l Silwet L-77, obtains infecting liquid.
3, take the liquid that infects that step 2 obtains, use and be stained with colored dip method and Columbia ecotype arabidopsis is carried out heredity turn Change, the most normally cultivate and gather in the crops seed (T1 is for seed).
4, take the seed of step 3 results, be seeded in containing on the 1/2MS solid medium containing 50mg/L hygromycin Cultivate, the resistant plant (T1 is for plant) screen 10 strain mounted blades, growing fine.Results resistant plant Seed (T2 is for seed).
5, take the seed of step 4 results, be seeded in containing on the 1/2MS solid medium containing 50mg/L hygromycin Cultivate, screening mounted blade, the resistant plant (T2 is for plant) grown fine.The seed of results resistant plant (T3 is for seed).
6, take the seed of step 5 results, normally sow and cultivate, obtaining T3 for plant.
7, sample for plant for plant and T3 for plant, T2 from T1 respectively, extract total serum IgE and reverse transcription is cDNA, To carrying out PCR qualification, (if obtaining the amplified production of 441bp, qualification result is the primer using F2 and R2 to form Positive;Otherwise, qualification result is negative).In T3 generation, obtain stable heredity to turn GmNMHC5 gene arabidopsis pure Close strain.
F2:5 '-ACCCATCACCTCATGAATCCG-3 ';
R2:5 '-AGACTGTGGCTGACTTAGCTG-3 '.
Three, the acquisition of empty carrier plant is turned
Replace recombiant plasmid pGUS-GmNMHC5 with carrier pGFPGUSplus, carry out step 2, obtain turning empty carrier and intend South mustard.
Four, character identification
Sample to be tested is as follows: the T3 of strain 1 (turning GmNMHC5 gene arabidopsis homozygous lines) is for seed, strain Be 2 (turning GmNMHC5 gene arabidopsis homozygous lines) T3 for seed, turn the T3 of empty carrier arabidopsis for seed, Columbia ecotype arabidopsis.
1, taking seed, in sterilized water, 4 DEG C are soaked 72h.
2, packet transaction
Long-day process group: after completing step 1, take seed, is seeded in equipped with in the nursery cave basin of seedling medium, 21 DEG C, cultivate under conditions of 16h illumination/8h dark;
Short-day sunshine treatment group: after completing step 1, takes seed, is seeded in equipped with in the nursery cave basin of seedling medium, 21 DEG C, cultivate under conditions of 8h illumination/16h dark;
Isopyknic soil and Vermiculitum are mixed to get by seedling medium.The size of nursery cave basin is 7cm × 7cm × 8cm.
Persistently carry out Phenotypic Observation, record flowering time (with the time of arabidopsis inflorescence seen from naked eyes as flowering time) And lotus throne number of blade when blooming.
Carry out three times repeating test, repeat every kind of sample to be tested in test every time and add up 15 strain plant, results averaged.
Under long-day processes, after sowing 30 days, Fig. 2 A is shown in by the photo of plant.
Under short-day sunshine treatment, after sowing 40 days, Fig. 2 B is shown in by the photo of plant.
The flowering time of every kind of sample to be tested and lotus throne number of blade when blooming are shown in Fig. 3.
Under long-day conditions: the flowering time of strain 1 is the 25th day after planting, and when blooming, the lotus throne number of blade is 8 Sheet;The flowering time of strain 2 is the 26th day after planting, and when blooming, the lotus throne number of blade is 9;Colombia is ecological The flowering time of type arabidopsis is the 33rd day after planting, and when blooming, the lotus throne number of blade is 14;Turn empty carrier and intend south The flowering time of mustard is after planting the 34th day, average 15 of lotus throne number of blade when blooming.Under the conditions of short-day: strain Be 1 flowering time be the 33rd day after planting, when blooming, the lotus throne number of blade is 9;The flowering time of strain 2 is The 34th day after planting, when blooming, the lotus throne number of blade is 10;The flowering time of Columbia ecotype arabidopsis is The 48th day after planting, when blooming, the lotus throne number of blade is 23;Turn the flowering time of empty carrier arabidopsis for after planting 49th day, average 24 of lotus throne number of blade when blooming.
Result above shows, compared with Columbia ecotype arabidopsis, turns blooming of GmNMHC5 gene arabidopsis bright Show in advance, i.e. GmNMHC5 albumen has the function promoting flowering of plant.

Claims (10)

1. a protein, is following (a) or (b):
A protein that () is made up of the aminoacid sequence shown in sequence in sequence table 1;
B the aminoacid sequence of sequence 1 through replacement and/or the disappearance of one or several amino acid residue and/or is added by () Add and with the protein derived by sequence 1 of flowering of plant time correlation.
2. the gene of protein described in coding claim 1.
3. gene as claimed in claim 2, it is characterised in that: described gene is following 1) or 2) or 3) DNA molecular:
1) coding region DNA molecular as shown in sequence 2 in sequence table;
2) under strict conditions with 1) the DNA sequence hybridization that limits and the DNA of coded plant flowering time associated protein Molecule;
3) with 1) or 2) DNA sequence that limits at least has 70%, at least has 75%, at least has 80%, extremely Have 85% less, at least have 90%, at least have 95%, at least have 96%, at least have 97%, at least have 98% or at least there is more than 99% homology and the DNA molecular of coded plant flowering time associated protein.
4. contain the recombinant expression carrier of gene described in Claims 2 or 3, expression cassette, transgenic cell line or restructuring Bacterium.
5. the application of protein described in claim 1, at least one in following (c1) to (c4):
(c1) florescence of plant is regulated and controled;
(c2) advance flowering period of plant is promoted;
(c3) flowering time of plant is regulated and controled;
(c4) flowering time of plant is promoted in advance.
Apply the most as claimed in claim 5, it is characterised in that: described plant is monocotyledon or dicotyledon.
7. the method cultivating transgenic plant, is by channel genes purpose plant described in Claims 2 or 3, To transgenic plant;At least one phenotype in described transgenic plant satisfied following (d1) to (d4):
(d1) bloom early than described purpose plant;
(d2) florescence is early than described purpose plant;
(d3) vegetative growth phase is shorter than described purpose plant;
(d4) generative growth phase is entered early than described purpose plant.
8. a plant breeding method, comprises the steps (e1) and/or (e1):
(e1) by increasing the activity of protein described in claim 1 in purpose plant, it is thus achieved that have following character Plant: bloom and be shorter than institute early than described purpose plant and/or florescence early than described purpose plant and/or vegetative growth phase State purpose plant and/or enter generative growth phase early than described purpose plant;
(e2) by promoting the expression of gene described in Claims 2 or 3 in purpose plant, it is thus achieved that have following character Plant: bloom and be shorter than early than described purpose plant and/or vegetative growth phase early than described purpose plant and/or florescence Described purpose plant and/or entrance generative growth phase are early than described purpose plant.
9. described in gene, claim 2 described in protein, claim 2 described in claim 1, gene, right are wanted Ask expression cassette application in plant breeding described in recombinant expression carrier described in 4 or claim 4.
Apply the most as claimed in claim 9, it is characterised in that: the purpose of described plant breeding carries for cultivating the florescence Front plant.
CN201610561640.9A 2016-07-15 2016-07-15 With the albumen and its encoding gene of flowering of plant time correlation and application Active CN105949295B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610561640.9A CN105949295B (en) 2016-07-15 2016-07-15 With the albumen and its encoding gene of flowering of plant time correlation and application

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610561640.9A CN105949295B (en) 2016-07-15 2016-07-15 With the albumen and its encoding gene of flowering of plant time correlation and application

Publications (2)

Publication Number Publication Date
CN105949295A true CN105949295A (en) 2016-09-21
CN105949295B CN105949295B (en) 2019-07-26

Family

ID=56901011

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610561640.9A Active CN105949295B (en) 2016-07-15 2016-07-15 With the albumen and its encoding gene of flowering of plant time correlation and application

Country Status (1)

Country Link
CN (1) CN105949295B (en)

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107312780A (en) * 2017-07-13 2017-11-03 中国农业大学 Chrysanthemum nuclear factor CmNF YB8 and its application in florescence control and childhood leaf regulating and controlling of quantities
CN108165557A (en) * 2017-11-23 2018-06-15 山东农业大学 Application of the wheat TaZCCT2 genes in the flowering of plant time is regulated and controled
CN109053871A (en) * 2018-08-09 2018-12-21 中国农业科学院作物科学研究所 Application of the AtBIX gene in the regulation flowering of plant time
CN109423492A (en) * 2017-08-21 2019-03-05 中国科学院遗传与发育生物学研究所 Application of the SlTOE1 gene in regulation tomato flowering time and yield
CN109988229A (en) * 2019-03-11 2019-07-09 西南大学 Wax plum CpFT gene and its application
CN110283240A (en) * 2019-06-19 2019-09-27 西南大学 Albumen and the application of wax plum CpUFO gene and its coding
CN110669119A (en) * 2019-10-16 2020-01-10 西南大学 EjAGL17 protein for regulating loquat flowering time and coding gene and application thereof
CN111072760A (en) * 2019-12-17 2020-04-28 西南大学 EjFRI gene for delaying loquat flowering time and encoding protein and application thereof
CN111171127A (en) * 2020-02-26 2020-05-19 浙江省农业科学院 Astragalus sinicus LHY gene and application thereof
CN112341529A (en) * 2020-11-17 2021-02-09 西南大学 Chimonanthus praecox CpFPA gene and protein coded by same and application of gene
WO2023168691A1 (en) * 2022-03-11 2023-09-14 Syngenta Crop Protection Ag Methods and compositions for modifying flowering time genes in plants

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101265293A (en) * 2007-03-16 2008-09-17 中国农业大学 Flowering time correlated albumen from arabidopsis, and coding gene and application thereof
CN102268080A (en) * 2010-06-02 2011-12-07 中国农业科学院作物科学研究所 Plant blossom related protein GmFTLa, coding gene thereof and application thereof
CN104710522A (en) * 2013-12-13 2015-06-17 中国农业科学院作物科学研究所 Protein related to flowering time of plant, and coding gene and application thereof

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101265293A (en) * 2007-03-16 2008-09-17 中国农业大学 Flowering time correlated albumen from arabidopsis, and coding gene and application thereof
CN102268080A (en) * 2010-06-02 2011-12-07 中国农业科学院作物科学研究所 Plant blossom related protein GmFTLa, coding gene thereof and application thereof
CN104710522A (en) * 2013-12-13 2015-06-17 中国农业科学院作物科学研究所 Protein related to flowering time of plant, and coding gene and application thereof

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
NCBI: "uncharacterized protein LOC100805092 [Glycine max]", 《GENBANK DATABASE》 *
WEI L等: "A Novel Sucrose-Regulatory MADS-Box Transcription Factor GmNMHC5 Promotes Root Development and Nodulation in Soybean (Glycine max [L.] Merr.)", 《INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES》 *

Cited By (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107312780B (en) * 2017-07-13 2021-08-03 中国农业大学 Chrysanthemum nuclear factor CmNF-YB8 and application thereof in flowering phase regulation and juvenile leaf number regulation
CN107312780A (en) * 2017-07-13 2017-11-03 中国农业大学 Chrysanthemum nuclear factor CmNF YB8 and its application in florescence control and childhood leaf regulating and controlling of quantities
CN109423492A (en) * 2017-08-21 2019-03-05 中国科学院遗传与发育生物学研究所 Application of the SlTOE1 gene in regulation tomato flowering time and yield
CN109423492B (en) * 2017-08-21 2021-01-15 中国科学院遗传与发育生物学研究所 Application of SlTOE1 gene in regulation and control of flowering time and yield of tomatoes
CN108165557A (en) * 2017-11-23 2018-06-15 山东农业大学 Application of the wheat TaZCCT2 genes in the flowering of plant time is regulated and controled
CN108165557B (en) * 2017-11-23 2021-06-22 山东农业大学 Application of wheat TaZCTC 2 gene in regulating and controlling flowering time of plants
CN109053871B (en) * 2018-08-09 2020-09-29 中国农业科学院作物科学研究所 Application of AtBIX gene in regulation and control of plant flowering time
CN109053871A (en) * 2018-08-09 2018-12-21 中国农业科学院作物科学研究所 Application of the AtBIX gene in the regulation flowering of plant time
CN109988229A (en) * 2019-03-11 2019-07-09 西南大学 Wax plum CpFT gene and its application
CN110283240B (en) * 2019-06-19 2021-02-19 西南大学 Chimonanthus praecox CpUFO gene and protein coded by same and application of gene
CN110283240A (en) * 2019-06-19 2019-09-27 西南大学 Albumen and the application of wax plum CpUFO gene and its coding
CN110669119A (en) * 2019-10-16 2020-01-10 西南大学 EjAGL17 protein for regulating loquat flowering time and coding gene and application thereof
CN111072760A (en) * 2019-12-17 2020-04-28 西南大学 EjFRI gene for delaying loquat flowering time and encoding protein and application thereof
CN111072760B (en) * 2019-12-17 2021-03-26 西南大学 EjFRI gene for delaying loquat flowering time and encoding protein and application thereof
CN111171127A (en) * 2020-02-26 2020-05-19 浙江省农业科学院 Astragalus sinicus LHY gene and application thereof
CN111171127B (en) * 2020-02-26 2021-07-30 浙江省农业科学院 Astragalus sinicus LHY gene and application thereof
CN112341529A (en) * 2020-11-17 2021-02-09 西南大学 Chimonanthus praecox CpFPA gene and protein coded by same and application of gene
WO2023168691A1 (en) * 2022-03-11 2023-09-14 Syngenta Crop Protection Ag Methods and compositions for modifying flowering time genes in plants

Also Published As

Publication number Publication date
CN105949295B (en) 2019-07-26

Similar Documents

Publication Publication Date Title
CN105949295B (en) With the albumen and its encoding gene of flowering of plant time correlation and application
CN107383179B (en) A kind of and plant stress tolerance correlative protein GsSLAH3 and its encoding gene and application
CN104232682B (en) Method for cultivating high-yield plant by overexpression of gma-miR156b
CN107827964A (en) A kind of transcription factor PwNAC2 related to plant stress tolerance and its encoding gene and application
CN110643618A (en) Jatropha curcas MYB transcription factor JcMYB16 gene and application thereof in improving drought resistance of plants
CN104903444B (en) Highly yielding ability nucleic acid, the method for preparing the increased genetically modified plants of yield, the method for increasing the yield of plant are assigned to plant
CN110066774A (en) Corn receptoroid kinase gene ZmRLK7 and its application
CN108570099A (en) The application of OsGLP2-1 albumen and its encoding gene in regulating and controlling seed dormancy
CN109232725A (en) Soybean C2H2 type single-zinc finger protein transcription factor and encoding gene and application
CN110540582A (en) Application of protein OrC1 in regulating color of rice husk and awn
CN109477091A (en) Construct and carrier for the conversion of gene implants
CN109468333A (en) Citrus laccase family gene CsiLAC4 and its application
CN106749580B (en) Plant salt tolerance GAP-associated protein GAP TaPUB15-D and its encoding gene and application
CN108048481A (en) Application of the RLI1 albumen in adjusting and controlling rice leaf angle
CN104513825B (en) Wheat salt-tolerant gene TaNAS1 and application thereof
CN106916826A (en) Paddy gene OsNF YC4 and its application
CN110684088B (en) Protein ZmbZIPa3 and application of coding gene thereof in regulating and controlling plant growth and development and stress tolerance
CN108690127B (en) Stress-resistance-associated protein TaMYB85 and coding gene and application thereof
CN104119432B (en) A kind of apocarya MADS-box class transcription factor CiMAD9 and encoding gene thereof and application
CN103602688B (en) Helianthus tuberosus L. Na<+>/H<+> reverse transport protein genes HtNHX1 and HtNHX2 and use thereof
CN107573411B (en) Application of wheat TaZIM1-7A protein in regulation and control of crop heading period
CN106995492B (en) Sucrose transporter and application thereof in regulation and control of plant male sterility
CN103348009B (en) A kind of method for preparing fertility-lowered plant
CN111440231B (en) Application of protein GmFULa in regulation and control of soybean plant type and yield
CN104651366B (en) Wheat microRNA408 and its encoding gene and application

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant