CN105948838B - A kind of compound lactobacillus microbial manure and its preparation and application - Google Patents
A kind of compound lactobacillus microbial manure and its preparation and application Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
Abstract
The invention discloses a kind of compound lactobacillus microbial manures, belong to technical field of fertilizers.The bio-feritlizer is prepared by four lactobacillus plantarum P-8, PB-1, PY-1 and SCI-02 fermentation.The present invention uses lactobacillus mixed fermentation, and special prescription and proportion are capable of providing the ability of high entire fermentation system production phenyllactic acid, and the risk for reducing plant introduced disease is further played during crop planting, delays or mitigates disease and aggravate process；Increase production plant, increases sugariness, improve the effect of mouthfeel；Beneficial microbe in soil is improved, soil-borne disease fungi is inhibited, improves soil microenvironment；And improve effect of the crop to the resistance of adverse circumstances.
Description
Technical field:
The invention belongs to technical field of fertilizers, and in particular to a kind of preparation and application of compound lactobacillus microbial manure.
Background technique:
Lactic acid bacteria is generally existing in natural environment as the member in agricultural benefit microorganism and Plant growth-promoting effect bacterium
A kind of safe and edible microorganism, exclusive advantage is played in agricultural cultivation and its quality safety.With to cream
The spy of the sour anti-pathogenic fungus characteristic research of bacterium goed deep into and the safeties aspects such as lactic acid bacteria degradation heavy metal, pesticide residue are studied
Rope, the vegetalitas agricultural product for reducing pesticide and chemical fertilizer plantation are more and more paid close attention to by people.Probiotics and soil
Intrinsic microorganism synergistic effect can inhibit foot end pathogenic fungus, bacterial pathogens and nematode or even ground in earth
Upper food grass insect damages caused by plant.Agricultural benefit probiotics are by lactic acid bacteria, saccharomycete, photosynthetic bacteria and to put
The composite probiotics preparations of the compositions such as line bacterium.Said preparation have promote plant health growth, increase plant products and photosynthetic rate,
Accelerate soil with organic matter decomposition and the release of nutriment, used conducive to plant absorption, reduction chemical agent, improvement soil matter
The effects of amount.
Lactic acid bacteria is one kind in Plant growth-promoting effect bacterium as probiotics, is generally acknowledged safety level (GRAS) bacterial strain, grinds
Study carefully discovery, lactobacillus plantarum, Lactobacillus rhamnosus, lactobacillus acidophilus and Lactobacillus delbrueckii are common in microbial manure production
Lactobacillus inoculation.There are the lactic acid bacterias such as lactobacillus, Pediococcus and Leuconostoc in apple.The lactic acid bacteria on grape surface is about
100CFU/g is gradually decreased with the extension of transporting procedures.The separation identification lactic acid bacteria research from different plants and agricultural product
It was found that lactic acid bacteria is the largest bacterial population (accounting for about 30%), wherein lactococcus is superiority bacteria spp.Lactic acid bacteria is applied to plant
Property agricultural product in there is the proliferation for inhibiting pathogenic bacteria, spoilage organisms, degrade heavy metal, pesticide residue reduce pesticide and chemical fertilizer
Use many advantages, such as, and be more and more valued by the people.
The present invention carries out production suppression to the lactobacillus plantarum being isolated from natural plants surface and rhizosphere for the above subject
Fungus matter inhibits a series of experiments such as rot disease fungi and pathogenic bacteria, filters out 1 plant and has and inhibits phytopathogenic fungi and common
The lactobacillus plantarum L.plantarum SCI-02 of food pollution pathogen.Then and with good rot fungi rejection ability
3 strains of lactic acid bacteria (L.plantarum P-8, L.plantarum PB-1, L.plantarum PY-1) mixed fermentation is prepared into multiple
Close lactobacillus micro-ecological fertilizer.Study compound lactobacillus micro-organism fertilizer improve soil microenvironment, promote plant growth,
Regulate and control the application of plant disease, enhancing stress resistance of plant and improvement vegetalitas quality of agricultural product etc., it is intended to promote lactic acid bacteria
Application development in agricultural product, and agricultural product quality and its edible safety are improved, to preferably improve people's health water
It is flat.
Summary of the invention:
The present invention provides a kind of compound lactobacillus microbial manure, the micro- life of compound lactobacillus to solve the above-mentioned problems
Object fertilizer the preparation method is as follows:
It (1) is 7% access fermentation medium by total inoculum concentration by four lactobacillus plantarum P-8, PB-1, PY-1 and SCI-02
Ferment, fermentation condition is as follows: the initial pH of fermentor is 7.0, and fermentation temperature is 30 DEG C, permanent pH 5.7-5.9, stream plus lye
After 4-5h, after spontaneous fermentation to pH 4.3-4.7, stream plus lye maintain fermentation liquid perseverance pH 5.7-5.9 to fermentation liquid itself pH not
It is fermentation termination when variation；
(2) after fermentation, by compound lactobacillus-fermencucumber liquid with after aseptic process sucrose and soy meal be uniformly mixed i.e.
Obtain compound lactobacillus microbial manure；
The inoculative proportion of described P-8, PB-1, PY-1 and SCI-02 are as follows: 1:1:1:2；
The fermentation medium composition is as follows: soyabean protein powder 20-40g, sucrose 40-80g, citric acid 0.7-1.0g,
K2HPO41-4g, sodium citrate 8-12g, Tween-80 1-3g, distilled water 1L, pH 7.0；
The additive amount and quality of fermentation broth volume ratio of the sucrose are 0.1%；
The additive amount and quality of fermentation broth volume ratio of the soy meal are 0.1%；
The lactobacillus plantarum P-8, deposit number CGMCC No.6312；
The lactobacillus plantarum PB-1, deposit number CGMCC No.5399；
The lactobacillus plantarum PY-1, deposit number CGMCC No.5358
The lactobacillus plantarum SCI-02 is specially lactobacillus plantarum (Lactobacillus plantarum) SCI-02,
The bacterial strain is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, address on December 24th, 2015
Are as follows: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica, postcode 100101, deposit number
CGMCC No.11931；
The SCI-02 bacterial strain is by inhibition phytopathogenic fungi (aspergillus parasiticus, aspergillus flavus, penicillium roqueforti, tomato early epidemic, sweet tea
Melon is withered, apple anthrax, muskmelon phytophthora) and enteropathic bacterium (Escherichia coli, mouse hurt salmonella, shigella flexneri, gold
Staphylococcus aureus, unit cell listeria spp) screening, and high yield broad-spectrum antibacterial substance-phenyllactic acid can be metabolized；
P-8, PB-1, PY-1 bacterial strain has good control effect to common Sapromyces.
1 L.plantarum SCI-02 fungi of table inhibits test
2 L.plantarum SCI-02 pathogenic bacteria of table inhibits test
Technical solution provided by the invention second is that by above-mentioned compound lactobacillus microbial manure be applied to production estimation in,
It is specific as follows: every 15-30 days, foliage-spray according to 100-200ml/ mu/time (will 100 times of fertilizer dilution after carry out using), root
Portion pour according to 500-1000ml/ mu/time (will 100 times of fertilizer dilute after carry out using).
The utility model has the advantages that
1, L.plantarum SCI-02 is added in Microecological compound fertilizer material provided by the present invention, which has good
Good bacteriostasis property, and high yield phenyllactic acid.
2, the present invention uses lactobacillus mixed fermentation, and special prescription and proportion are capable of providing high L.plantarum SCI-
02 and entire fermentation system production phenyllactic acid ability.
3, Microecological compound fertilizer material a. provided by the invention has the risk for reducing plant introduced disease, has to plant and protects
Shield effect can delay or mitigate disease and aggravate process；B. having increases production plant, increases sugariness (sugar content increases), improves mouth
The effect of sense；C. be conducive to improve beneficial microbe in soil, inhibit soil-borne disease fungi, improve the effect of soil microenvironment
Fruit: fungi in soil is reduced；Increase actinomyces in soil, bacillus and total number of bacteria increase (increase separately 6.88%,
10.70% and 46.06%)；D. crop is improved to the resistance of adverse circumstances.
Detailed description of the invention
Fig. 1: compound lactobacillus microbial manure can reduce the risk of cucumber plant introduced disease
Fig. 2: compound lactobacillus microbial manure can delay cucumber plant disease to aggravate process
Fig. 3: compound lactobacillus microbial manure can prevent, alleviate cucumber plant disease
Fig. 4: compound lactobacillus microbial manure can be improved tomato production
Fig. 5: compound lactobacillus microbial manure can be improved western red sugar content, improve tomato taste quality and soil reason
Change character
Fig. 6: compound lactobacillus microbial manure can significantly improve western red plant root development growth
Fig. 7: compound lactobacillus microbial manure can remarkably promote tomato plant growth
Fig. 8: compound lactobacillus microbial manure can improve soil microenvironment
Fig. 9: compound lactobacillus microbial manure can promote soil with organic matter to decompose and nitrogen and phosphorus nutrient release
Specific embodiment:
Below with reference to embodiment, the invention will be further described；Following embodiment is only illustrative, and the present invention is not
It is limited to these embodiments.
Experimental method in following embodiments is unless otherwise instructed conventional method.
Compound lactobacillus (L.plantarum P-8, L.plantarum PB-1, L.plantarum PY-1 and
L.plantarum SCI-02) mixed fermentation prepares microbial manure and its application.
Embodiment 1, test method
1.1 actication of culture
Be freeze-dried save bacterial strain L.plantarum P-8, L.plantarum PB-1, L.plantarum PY-1 and
L.plantarum SCI-02 strain is inoculated in MRS fluid nutrient medium, 37 DEG C of constant temperature incubation 18h, is passed on 2 times.
1.2 count plate
Generally use pour plate counting method, operating procedure are as follows: according to national standard method, shake up fermentation liquid, draw 1mL fermentation
Liquid is placed in the sterile saline of 9mL, is carried out 10 times of dilutions, is then successively diluted to required gradient.By last dilution gradient
After tube dilution vibration is even, draws 1mL dilution and pour into plate, to culture medium is entered, shake up, set solidification, in 37 DEG C of incubators
Cultivate 48h.Plate does two gradients respectively, and each gradient two parallel.
Measure viable count of lactobacillus culture medium: MRS solid medium
1.3pH measurement: fermentation liquid pH value is directly measured using accurate pH meter
1.4 phenyllactic acid assays (LC-MS method): LC condition: chromatographic column BEH C18；Flow 0.4ml/min；Column temperature: 45
℃；Sample volume: 4 μ l；Mobile phase A :+0.1% formic acid of water；Mobile phase B :+0.1% formic acid of acetonitrile；Gradient elution 3min.MS item
Part: positive ion mode, ESI ion source, orifice potential 20V；Impact energy 4V.
Influence of 2 inoculative proportion of embodiment to mixed fungus fermentation
Study different vaccination ratio, the influence to strain mixed fermentation.
Culture medium prescription is as follows: soyabean protein powder 30g, sucrose 60g, citric acid 0.8g, K2HPO42g, sodium citrate 10g,
Tween-80 2g, distilled water 1L, pH 7.0；
The initial pH of fermentor: being 7.0, and fermentation temperature is 30 DEG C, permanent pH 5.7-5.9, and stream plus lye maintain fermentation liquid perseverance pH
It is fermentation termination when 5.7-5.9 to fermentation liquid itself pH no longer changes；
A. as L.plantarum P-8, L.plantarum PB-1, L.plantarum PY-1 and L.plantarum
Total inoculum concentration of SCI-02 is 6% (inoculative proportion 1:1:1:1), and 30 DEG C, 80r/min fermentation, measuring viable count of lactobacillus is
0.72×1010CFU/mL；
B. as L.plantarum P-8, L.plantarum PB-1, L.plantarum PY-1 and L.plantarum
Total inoculum concentration of SCI-02 is 6% (inoculative proportion 2:1:1:1), and 30 DEG C, 80r/min fermentation, measuring viable count of lactobacillus is
0.84×1010CFU/mL；
C. as L.plantarum P-8, L.plantarum PB-1, L.plantarum PY-1 and L.plantarum
Total inoculum concentration of SCI-02 is 7% (inoculative proportion 1:1:1:2), and 30 DEG C, 80r/min fermentation, measuring viable count of lactobacillus is
1.03×1010CFU/mL；
According to experimental result, when L.plantarum P-8, L.plantarum PB-1, L.plantarum PY-1 and
Total inoculum concentration of L.plantarum SCI-02 is 7% (inoculative proportion 1:1:1:2), conducive to compound lactobacillus microbial manure
Fermentation.
Influence of 3 zymotechnique of embodiment to phenyllactic acid in fermentation liquid
Different fermentations technique is studied, the influence to antibacterial substance in mixed fermentation liquid (phenyllactic acid):
Fermentation medium: soyabean protein powder 30g, sucrose 60g, citric acid 0.8g, K2HPO42g, sodium citrate 10g, spits
Temperature -80 2g, distilled water 1L, pH 7.0.L.plantarum P-8, L.plantarum PB-1, L.plantarum PY-1 and
Total inoculum concentration of L.plantarum SCI-02 is 7% (inoculative proportion 1:1:1:2)；
A. the initial pH of fermentor is 7.0, and fermentation temperature is 30 DEG C, permanent pH 5.9, and whole process stream adds lye to fermentation liquid itself
It is fermentation termination when pH no longer changes；Broad-spectrum antibacterial substance-phenyllactic acid content in sample detection fermentation liquid is 25.7mg/L,
Viable count is 1.10 × 1010CFU/mL；
Other conditions are constant, access strain be changed to L.plantarum SCI-02 inoculum concentration be 7% when, experimental result are as follows:
Phenyllactic acid content is 8.6mg/L, and viable count is 1.01 × 1010CFU/mL。
Other conditions are constant, access strain be changed to L.plantarum PY-1 inoculum concentration be 7% when, experimental result are as follows: benzene
Lactic acid content is 3.2mg/L, and viable count is 0.98 × 1010CFU/mL
Other conditions are constant, access strain be changed to L.plantarum P-8 inoculum concentration be 7% when, experimental result are as follows: benzene cream
Acid content is 8.3mg/L, and viable count is 1.21 × 1010CFU/mL
Other conditions are constant, access strain be changed to L.plantarum PB-1 inoculum concentration be 7% when, experimental result are as follows: benzene
Lactic acid content is 3.7mg/L, and viable count is 0.87 × 1010CFU/mL
B. the initial pH of fermentor is 7.0, and fermentation temperature is 30 DEG C, permanent pH 5.9, after stream adds lye 7h, spontaneous fermentation to pH
(about pH4.2-4.5) is considered as fermentation termination until when no longer changing；Broad-spectrum antibacterial substance-benzene cream in sample detection fermentation liquid
Acid content is 30.6mg/L, and viable count is 0.72 × 1010CFU/mL；
Other conditions are constant, access strain be changed to L.plantarum SCI-02 inoculum concentration be 7% when, experimental result are as follows:
Phenyllactic acid content is 10.3mg/L, and viable count is 0.71 × 1010CFU/mL；
Other conditions are constant, access strain be changed to L.plantarum PY-1 kind amount be 7% when, experimental result are as follows: benzene cream
Acid content is 5.2mg/L, and viable count is 0.78 × 1010CFU/mL；
Other conditions are constant, access strain be changed to L.plantarum P-8 kind amount be 7% when, experimental result are as follows: phenyllactic acid
Content is 8.1mg/L, and viable count is 0.65 × 1010CFU/mL；
Other conditions are constant, access strain be changed to L.plantarum PB-1 kind amount be 7% when, experimental result are as follows: benzene cream
Acid content is 4.7mg/L, and viable count is 0.72 × 1010CFU/mL。
C. the initial pH of fermentor is 7.0, and fermentation temperature is 30 DEG C, permanent pH 5.9, and after stream adds lye 4.5h, spontaneous fermentation is extremely
After pH 4.5, stream plus lye are fermentation termination when fermentation liquid perseverance pH 5.9 to fermentation liquid itself pH being maintained no longer to change.Sampling inspection
Broad-spectrum antibacterial substance-phenyllactic acid the content surveyed in fermentation liquid is 49.2mg/L, and viable count is 1.06 × 1010CFU/mL；
Other conditions are constant, access strain be changed to L.plantarum SCI-02 kind amount be 7% when, experimental result are as follows: benzene
Lactic acid content is 15.3mg/L, and viable count is 0.96 × 1010CFU/mL；
Other conditions are constant, access strain be changed to L.plantarum PY-1 kind amount be 7% when, experimental result are as follows: benzene cream
Acid content is 10.1mg/L, and viable count is 0.78 × 1010CFU/mL；
Other conditions are constant, access strain be changed to L.plantarum P-8 kind amount be 7% when, experimental result are as follows: phenyllactic acid
Content is 12.4mg/L, and viable count is 1.01 × 1010CFU/mL；
Other conditions are constant, access strain be changed to L.plantarum PB-1 kind amount be 7% when, experimental result are as follows: benzene cream
Acid content is 9.8mg/L, and viable count is 0.79 × 1010CFU/mL。
D. the initial pH of fermentor is 7.0, and fermentation temperature is 30 DEG C, and spontaneous fermentation is terminal for 24 hours.In sample detection fermentation liquid
Broad-spectrum antibacterial substance-phenyllactic acid content be 50.1mg/L, viable count be 0.50 × 1010CFU/mL；
Other conditions are constant, access strain be changed to L.plantarum SCI-02 kind amount be 7% when, experimental result are as follows: benzene
Lactic acid content is 12.5mg/L, and viable count is 0.45 × 1010CFU/mL；
Other conditions are constant, access strain be changed to L.plantarum PY-1 kind amount be 7% when, experimental result are as follows: benzene cream
Acid content is 12.1mg/L, and viable count is 0.56 × 1010CFU/mL；
Other conditions are constant, access strain be changed to L.plantarum P-8 kind amount be 7% when, experimental result are as follows: phenyllactic acid
Content is 9.4mg/L, and viable count is 0.42 × 1010CFU/mL；
Other conditions are constant, access strain be changed to L.plantarum PB-1 kind amount be 7% when, experimental result are as follows: benzene cream
Acid content is 10.1mg/L, and viable count is 0.51 × 1010CFU/mL。
Conclusion: in conclusion through different vaccination ratio, different fermentations technical study considers 4 strains of lactic acid bacteria mixed fermentations
When, the influence to viable count of lactobacillus and antibacterial substance yield is comprehensive preferred: L.plantarum P-8, L.plantarum
Total inoculum concentration of PB-1, L.plantarum PY-1 and L.plantarum SCI-02 are 7% (inoculative proportion 1:1:1:2)
The initial pH of fermentor is 7.0, and fermentation temperature is 30 DEG C, permanent pH 5.7-5.9, after stream adds lye 4-5h, spontaneous fermentation
To pH 4.3-4.7, it is best that stream plus lye, which are fermentation termination when fermentation liquid perseverance pH 5.7-5.9 to pH being maintained no longer to change,
Zymotechnique.
The preparation of 4 compound lactobacillus microbial manure of embodiment:
It (1) is 7% by total inoculum concentration by four lactobacillus plantarum P-8, PB-1, PY-1 and SCI-02, inoculative proportion are as follows: 1:
1:1:2, access fermentation medium ferment；
Fermentation condition is as follows: the initial pH of fermentor: being 7.0, fermentation temperature is 30 DEG C, permanent pH 5.7-5.9, stream plus lye
After 4h, after spontaneous fermentation to pH 4.3-4.7, stream plus lye maintain fermentation liquid perseverance pH 5.7-5.9 to fermentation liquid itself pH no longer
It is fermentation termination when variation；
Fermentation medium composition is as follows: soyabean protein powder 20g, sucrose 40g, citric acid 0.7g, K2HPO41g, sodium citrate
8g, Tween-80 1g, distilled water 1L, pH 7.0；
(2) after fermentation, by after compound lactobacillus-fermencucumber liquid and aseptic process sucrose and soy meal after mixing
Packaging refrigerates up to compound lactobacillus microbial manure；The additive amount and quality of fermentation broth volume ratio of sucrose are 0.1%；Soy meal
Additive amount be 0.1%.
The preparation of 5 compound lactobacillus microbial manure of embodiment:
It (1) is 7% by total inoculum concentration by four lactobacillus plantarum P-8, PB-1, PY-1 and SCI-02, inoculative proportion are as follows: 1:
1:1:2, access fermentation medium ferment；
Fermentation condition is as follows: the initial pH of fermentor: being 7.0, fermentation temperature is 30 DEG C, permanent pH 5.7-5.9, stream plus lye
After 5h, after spontaneous fermentation to pH 4.3-4.7, stream plus lye maintenance fermentation liquid perseverance pH 5.7-5.9 to pH are not ferment in variation
Terminal；
Fermentation medium composition is as follows: soyabean protein powder 40g, sucrose 80g, citric acid 1.0g, K2HPO44g, sodium citrate
12g, Tween-80 3g, distilled water 1L, pH 7.0；
(2) after fermentation, by after compound lactobacillus-fermencucumber liquid and aseptic process sucrose and soy meal after mixing
Packaging refrigerates up to compound lactobacillus microbial manure；The additive amount and quality of fermentation broth volume ratio of sucrose are 0.1%；Soy meal
Additive amount be 0.1%.
The preparation of 6 compound lactobacillus microbial manure of embodiment:
It (1) is 7% by total inoculum concentration by four lactobacillus plantarum P-8, PB-1, PY-1 and SCI-02, inoculative proportion are as follows: 1:
1:1:2, access fermentation medium ferment；
Fermentation condition is as follows: the initial pH of fermentor: being 7.0, fermentation temperature is 30 DEG C, permanent pH 5.7-5.9, stream plus lye
After 4.5h, after spontaneous fermentation to pH 4.3-4.7, stream plus lye maintenance fermentation liquid perseverance pH 5.7-5.9 to pH are not in variation
Fermentation termination；
Fermentation medium composition is as follows: soyabean protein powder 30g, sucrose 60g, citric acid 0.8g, K2HPO42g, sodium citrate
10g, Tween-80 2g, distilled water 1L, pH 7.0；
(2) after fermentation, by after compound lactobacillus-fermencucumber liquid and aseptic process sucrose and soy meal after mixing
Packaging refrigerates up to compound lactobacillus microbial manure；The additive amount and quality of fermentation broth volume ratio of sucrose are 0.1%；Soy meal
Additive amount be 0.1%.
The application of 7 compound lactobacillus microbial manure of embodiment
A. 6 gained compound lactobacillus micro-organism fertilizer of embodiment is inhibited applied to cucumber disease
Test grouping: cucumber plant is randomly divided into test group and control group, and every group each 9 plants.
Test method: test group cucumber is every 15 days foliage-sprays and root irrigation compound lactobacillus microbial manure one
It is secondary.Foliage-spray according to 150ml/ mu/time (will 100 times of fertilizer dilution after carry out using), root pour 1000ml/ mu/time (general
100 times of fertilizer dilution after carry out using).
Experimental observation: observing and recording the disease of cucumber plant every 15d, a situation arises and the growing state of plant.
Trial: test is whole not to use any pesticide and antibacterial substance, does not apply any fertilizer, test group and right
It is identical according to group cucumber administrative situation.
Test result:
Compound lactobacillus microbial manure can reduce the probability of cucumber plant disease infection and disease delayed to aggravate process,
It is caught an illness probability 33.4% using plant is reduced after 15d, using reduces degree of disease 25% after 30d.Compound lactobacillus microorganism fertilizer
Expect that there is the risk for reducing cucumber plant introduced disease, there is protective effect to cucumber plant；It can delay or mitigate cucumber plant
Disease aggravates process, has protective effect to cucumber plant.See Fig. 1-3.
B. 5 gained compound lactobacillus micro-organism fertilizer of embodiment is increased production applied to tomato
Test grouping: tomato is randomly divided into test group and control group, and every group each 0.5 mu.
Test method: test group tomato is every 15 days foliage-sprays and root irrigation compound lactobacillus microbial manure one
It is secondary.Foliage-spray according to 150ml/ mu/time (will 100 times of fertilizer dilution after carry out using), root pour 1000ml/ mu/time (general
100 times of fertilizer dilution after carry out using).
Experimental observation: the growing state and yield of tomato plant are observed and recorded daily.
Trial: test is whole not to use any pesticide and antibacterial substance, test group and control group tomato management
Situation is identical.
Test result:
Lactic acid bacteria fertilizer can remarkably promote tomato root system development, accelerate plant strain growth, and it is average to improve tomato single plant
Yield, test group produce compared with control group strain and improve 14.34%.The sugar content of growing tomatoes soil and tomato is carried out after test
It was found that: test group pH6.57, substantially less than control group (pH6.97), vegetable growth Optimal pH are 6.5-6.8, use cream
Sour bacterial manure material has certain improvement to soil.In addition, the sugariness (sugar content) of tomato increases after using lactic acid bacteria fertilizer
Height, mouthfeel improve.There is improvement result using quality of the lactic acid bacteria fertilizer to tomato.See Fig. 4-7.
C., 4 gained compound lactobacillus micro-organism fertilizer of embodiment is applied to the improvement of soil microenvironment and fertilizer efficiency
Test grouping: tomato, cucumber are randomly divided into test group and control group, and every group each 1 mu.
Test method: test group is primary every 15 days foliage-sprays and root irrigation compound lactobacillus microbial manure.Leaf
Face spray according to 150ml/ mu/time (will 100 times of fertilizer dilution after carry out using), root pour 700ml/ mu/time (by fertilizer
100 times dilution after carry out using).
Soil K+adsorption: test early period and post-collection test group and control group soil sample detect the microorganism in soil
Content and fertility index.
Trial: test is whole not to use any pesticide and antibacterial substance, test group and control group tomato management
Situation is identical.
Test result:
After growing tomatoes use compound lactobacillus microbial manure, Soil Microbes discovery: fungi number in soil
Amount substantially reduces and (reduces 28.57%)；Actinomyces in soil, bacillus and total number of bacteria increase (increase separately 6.88%,
10.70% and 46.06%).Compound lactobacillus microbial manure is conducive to improve beneficial microbe in soil, inhibits soil-borne disease
Fungi improves soil microenvironment.
Compound lactobacillus microbial manure can substantially reduce soil with organic matter and (refer to: carbon containing organic compound in soil
Object) content (reducing 27.31%), available phosphorus contents (reducing 33.67%) and alkaline hydrolysis nitrogen content (reducing 27.68%).Be conducive to
Soil with organic matter is promoted to decompose and nitrogen and phosphorus nutrient release, the absorption of promotion Plant To Nutrient element.See Fig. 8-9.
D. 6 gained compound lactobacillus micro-organism fertilizer of embodiment is tested applied to stress resistance of plant
Test grouping: tomato and rapeseed plants are randomly divided into test group and control group, and every group each 1 mu.
Test method: test group is primary every 15 days foliage-sprays and root irrigation compound lactobacillus microbial manure.Leaf
Face spray according to 150ml/ mu/time (will 100 times of fertilizer dilution after carry out using), root pour 1000ml/ mu/time (by fertilizer
100 times dilution after carry out using).
Experimental observation: the growing state of tomato and rapeseed plants is observed and recorded daily.
Trial: test is whole not to use any pesticide and antibacterial substance, does not apply any fertilizer, test group and right
It is identical according to group administrative situation.
Test result:
1) rape test discovery: when 2 month of winter low temperature, since temperature is lower within the scope of 1 meter of greenhouse south, in greenhouse
The rape of north plantation, plant strain growth significant difference.Rape within the scope of 1 meter of the control group greenhouse south plant height that is averaged is
12.1cm, north rape are averaged plant height as 14.8cm (the two differs 2.7cm, significant difference)；1 meter of test group greenhouse south range
Interior rape is averaged plant height as 14.2cm, and north rape is averaged plant height as 14.7cm (the two differs 0.5cm, and difference is not significant).
Rape can be enhanced to the resistance of low temperature by spraying compound lactobacillus microbial manure.
2) tomato experiment discovery: when summer August part high temperature, when greenhouse temperature is higher than 38 degree, the tomato leaf of test block
Normally, there is the phenomenon that is Protected from Heat in the blade of check plot, and rolls up on blade.Illustrate that west can be enhanced by spraying compound lactobacillus microbial manure
Resistance of the red persimmon to high temperature.
Interpretation of result: compound lactobacillus microbial manure can remarkably promote promote tomato plant aerial part (plant height,
Ground diameter) growth and root system development, improve tomato and be averaged single plant yield, test group produces raising 14.34% compared with control group strain strain.
Compound lactobacillus microbial manure is conducive to improve beneficial microbe in soil, inhibits soil-borne disease fungi, improves the micro- life of soil
State environment.Compound lactobacillus microbial manure may advantageously facilitate soil with organic matter and decompose and nitrogen and phosphorus nutrient release, promotion plant
Absorption to nutrient.In addition, plant enhances the resistance of high temperature, low temperature and disease after using lactic acid bacteria fertilizer,
Be conducive to the healthy growth of plant.In addition, the sugariness (sugar content) of tomato increases, mouthfeel improves, and has to the quality of tomato
There is improvement result.See attached drawing 4-9.
Conclusion:
Fermentation liquid is made through composite fermentation in 4 strains of lactic acid bacteria, and viable count of lactobacillus reaches 1.06 × 1010CFU/mL or more.It is mixed
It closes fermentation liquid and compound lactobacillus microbial manure is made in carrier after mixing.
Compound lactobacillus microbial manure can remarkably promote growth and the root system hair of aboveground vegetation part (plant height, ground diameter)
It educates, improves crop yield.Compound lactobacillus microbial manure is conducive to improve beneficial microbe in soil, inhibits soil-borne disease true
Bacterium improves soil microenvironment.Compound lactobacillus microbial manure may advantageously facilitate soil with organic matter decomposition and nitrogen phosphorus is supported
Divide release, promotes the absorption of Plant To Nutrient element.In addition, plant is to high temperature, low temperature and disease after using lactic acid bacteria fertilizer
Resistance enhanced, be conducive to the healthy growth of plant.In addition, fruit sweetness (sugar content) increases, mouthfeel improves, to quality
With improvement result.
Claims (6)
1. a kind of compound lactobacillus microbial manure, which is characterized in that the preparation method of the compound lactobacillus microbial manure
It is as follows:
It (1) is that 7% access fermentation medium carries out by total inoculum concentration by four lactobacillus plantarum P-8, PB-1, PY-1 and SCI-02
Fermentation, fermentation condition are as follows: the initial pH of fermentor is 7.0, and fermentation temperature is 30 DEG C, permanent pH 5.7-5.9, stream plus lye 4-5h
Afterwards, after spontaneous fermentation to pH 4.3-4.7, stream plus lye maintain fermentation liquid perseverance pH 5.7-5.9 to fermentation liquid itself pH not change
When be fermentation termination；
The lactobacillus plantarum P-8, deposit number CGMCC No.6312；
The lactobacillus plantarum PB-1, deposit number CGMCC No.5399；
The lactobacillus plantarum PY-1, deposit number CGMCC No.5358；
The lactobacillus plantarum SCI-02, deposit number CGMCC No.11931.
(2) after fermentation, by compound lactobacillus-fermencucumber liquid with after aseptic process sucrose and soy meal be uniformly mixed up to multiple
Close lactic acid bacteria microbe fertilizer.
2. a kind of compound lactobacillus microbial manure as described in claim 1, which is characterized in that the lactobacillus plantarum P-8,
The inoculative proportion of PB-1, PY-1 and SCI-02 are as follows: 1:1:1:2.
3. a kind of compound lactobacillus microbial manure as described in claim 1, which is characterized in that the fermentation medium composition
It is as follows: soyabean protein powder 20-40g, sucrose 40-80g, citric acid 0.7-1.0g, K2HPO41-4g, sodium citrate 8-12g, spits
Temperature -80 1-3g, distilled water 1L, pH 7.0.
4. a kind of compound lactobacillus microbial manure as described in claim 1, which is characterized in that the additive amount of the sucrose with
Quality of fermentation broth volume ratio is 0.1%；The additive amount and quality of fermentation broth volume ratio of the soy meal are 0.1%.
5. application of compound lactobacillus microbial manure during crop planting described in claim 1.
6. application of the compound lactobacillus microbial manure as claimed in claim 5 during crop planting, feature exist
In, every 15-30 days, by 100 times of fertilizer dilution after, foliage-spray according to 100-200ml/ mu/time use, root pouring according to
500-1000ml/ mu/time uses.
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| CN111996146B (en) * | 2020-08-31 | 2021-06-15 | 山东宝来利来生物工程股份有限公司 | Lactobacillus plantarum for high yield of phenyllactic acid and application thereof |
| CN114231458B (en) * | 2021-12-24 | 2023-03-24 | 青岛尚德生物技术有限公司 | Compound microbial agent for improving sugar-acid ratio of melons and fruits as well as preparation method and application of compound microbial agent |
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