CN105940895A - SOD (Superoxide Dismutase) Diospyros kaki fruits and production method therefor - Google Patents
SOD (Superoxide Dismutase) Diospyros kaki fruits and production method therefor Download PDFInfo
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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Abstract
The invention belongs to the technical field of cultivation and production of fruits and particularly discloses SOD (Superoxide Dismutase) Diospyros kaki fruits and a production method therefor. The content of SOD in the SOD Diospyros kaki fruits is 31IU/g to 41IU/g. The production method for the SOD Diospyros kaki fruits comprises the step of spraying an SOD enzyme preparation for fruit trees to crowns of Diospyros kaki trees or/and injecting the SOD enzyme preparation for the fruit trees to trunks of the Diospyros kaki trees from a squaring stage of the Diospyros kaki trees to a fruit color changing stage of the Diospyros kaki trees under the condition of conventional cultivation management. The SOD Diospyros kaki fruits produced by the production method have the advantages that the excellent characteristics of the original Diospyros kaki fruits are kept, the content and activity of the SOD in the Diospyros kaki fruits are obviously improved, and the content of the SOD can reach 41IU/g to the maximum and is increased by 75.97% compared with that of the ordinary Diospyros kaki fruits; in addition, the content of soluble solids, the content of vitamin C and weight of single fruit in the SOD Diospyros kaki fruits are obviously increased. The production method for the SOD Diospyros kaki fruits, disclosed by the invention, is simple, is low in cost and has a broad market prospect.
Description
Technical field
The invention belongs to fruit cultivation production technical field, be specifically related to a kind of SOD Fructus Kaki and production method thereof.
Background technology
SOD i.e. superoxide dismutase, is indispensable in human body to have special bioactive enzyme, it
Free radical can be decomposed, be the safety net that cell do not encroached on by free radical that protects the health, when it meets with free radical,
Free radical will become harmless hydrone and oxygen molecule, and then improve the immunity of human body, therefore,
SOD is effective scavenger of harmful free radicals in organism, human body is had prevention and treat multiple disease,
The effect such as looks improving and the skin nourishing, slow down aging.International biochemical committee, Federal Food management board are called " anti-
Decline the factor ", " beauty treatment tender son ".Taken long-term can strengthen body immunity, promotes oxidation resistance, plays anti-
Sick antibacterial grade acts on.Test proves, SOD is applied to fruit tree can be effectively increased its SOD enzyme activity, and activation is planted
Related gene in object, improves fruit vegetable nutrient and is worth so that it is possess the effects such as antioxygen health care, also can extend it
Shelf-life.
Fructus Kaki (Diospyros kaki Thunb.) is Ebenaceae (Ebenaceae) Diospyros (Diospyros L.) deciduous tree
Or shrub, ecological amplitude is wide, adaptability is extremely strong, and cultivation in a year is benefited for many years, is the important species shaken off poverty and set out on the road to prosperity of mountain area,
Ye Shi China tradition fruit, recently as country to woody grain and oil energetically advocate and people's living standard not
Disconnected raising, the development of Fructus Kaki industry is subject to people's attention again.At present, the industry development of China's Fructus Kaki is mainly deposited
In following problem: Fructus Kaki produces predominantly petty-farmer management, non-intensive farming, extensive management, and quality is low,
To the fine work day by day risen, functional fruit demand understanding deficiency；Production is only focused on yield, ignores fine work Fructus Kaki
Fruit and the production of functional fruit, overall functionality Fructus Kaki is few, and commodity performance is low；Produce and merchandise sales at Fructus Kaki
In, the most only proposing the rough concept of organic Fructus Kaki, the concept of new type functional high-quality Fructus Kaki is not yet suggested
With；And, in the cultivation of functional Fructus Kaki and method, lack effective thinking, technical measures and approach.
Therefore, develop, produce have the functional Fructus Kaki of high activity content be of Fructus Kaki industry development new and have
The direction of great potential.SOD fruit is on the premise of keeping or improving the original quality of fruit, by life
SOD enzyme is placed in harmless bacillus cereus by thing technology, is proceeded in fruit by bacillus cereus again,
In making fruit, activity SOD content increases.The most nationwide common SOD fruit is mainly Fructus Mali pumilae, on a small quantity
Apply in the products such as Fructus Vitis viniferae, Fructus Jujubae, vegetable.In whole nation Fructus Kaki produces, have not yet seen relevant SOD
The relevant report of Fructus Kaki product.Therefore, Study of functional SOD Fructus Kaki, tradition Fructus Kaki is carried out quality improvement pair
The development meaning of Fructus Kaki industry is the most great.Persimmon is different from other common fruit tree, has that tree height is big, Sheng Guo
Phase length, fruit are relatively big, peel and the feature such as blade is thicker, so the application combines persimmon and the life of Fructus Kaki self
Thing and physiologic characteristic, have studied the production method of a kind of SOD Fructus Kaki.
Summary of the invention
For problems of the prior art, it is an object of the invention to provide a kind of SOD Fructus Kaki and production thereof
Method.The SOD Fructus Kaki obtained by the present invention not only maintains the merit of original Fructus Kaki, and Fructus Kaki
Middle SOD, soluble solid and ascorbic content are significantly improved；This SOD Fructus Kaki has increasing simultaneously
Strong body immunity and effect of slow down aging, the SOD enzyme that still can keep higher in deep-processing process is lived.
The technical solution used in the present invention is:
A kind of SOD Fructus Kaki, in described SOD Fructus Kaki, the content of SOD is 31-41IU/g.
The production method of above-mentioned SOD Fructus Kaki, described production method is under conventional cultivation management condition, from Fructus Kaki
Tree starts to fruit colour-change period squaring period, to persimmon tree crown spraying fruit tree by SOD enzyme preparation or/and trunk injection
Fruit tree SOD enzyme preparation；Wherein, described fruit tree is scientific and technological purchased from Lingbao City of Henan Province benefit treasured by SOD enzyme preparation
Co., Ltd；
The described tree crown spraying fruit tree method of SOD enzyme preparation is: every mu every time by fruit tree SOD enzyme preparation
75-100g, adds 90-140kg water and dissolves, after being uniformly mixed spray persimmon tree crown, from existing
Flower bud phase to fruit colour-change period sprays 5 times altogether, every minor tick 30-35 days；
The described trunk injection fruit tree method of SOD enzyme preparation comprises the following steps:
(1) boring: holing in the region far from ground 10-20cm, the persimmon rhizome portion, drilling hole amount is
2-3, aperture is 4.5-5.0cm, and hole depth reaches heart, and boring and trunk are the angle of 30-45 degree；
(2) injection: every mu, every time by fruit tree SOD enzyme preparation 90-110g, adds 3-4kg water and dissolves,
It is uniformly mixed in backward boring injection, injects 5 times, between per injection from squaring period altogether to fruit colour-change period
Every 30-35 days；
(3) sealing of hole: after per injection terminates, blocks boring with fruit tree wound healing agent or dry branch, with
Protection boring, promotes healing.
According to above-mentioned production method, it is preferable that the described tree crown spraying fruit tree method of SOD enzyme preparation is:
Every mu, every time by fruit tree SOD enzyme preparation 100g, adds 120kg water and dissolves, right after being uniformly mixed
Persimmon tree crown is sprayed, and sprays altogether 5 times from squaring period to fruit colour-change period, wherein, sprays 1 squaring period
Secondary, young fruit period is sprayed 1 time, and fruit expanding period is sprayed 2 times, and fruit colour-change period sprays 1 time, every minor tick
30-35 days.
According to above-mentioned production method, it is preferable that the method for described trunk injection fruit tree SOD enzyme preparation is:
Every mu, every time by fruit tree SOD enzyme preparation 110g, adds 4kg water and dissolves, be uniformly mixed backward brill
Hole is injected, injects altogether 5 times from squaring period to fruit colour-change period, wherein, inject 1 squaring period, young fruit period
Injecting 1 time, fruit expanding period is injected 2 times, and fruit colour-change period injects 1 time, every minor tick 30-35 days.
The positive beneficial effect of the present invention:
(1) the SOD Fructus Kaki that the present invention produces not only maintains the merit of original Fructus Kaki, and in Fructus Kaki
SOD, soluble solid and ascorbic content significantly improve, and wherein, the content of SOD reaches as high as
41IU/g, compared with common Fructus Kaki, improves 75.97%, soluble solid content, Vitamin C content,
Single fruit weight best result has not reached 22.6%, 48.4mg/100g and 202.4g, carries respectively compared with common Fructus Kaki
High by 10.78%, 7.32% and 9.7%.
(2) the SOD Fructus Kaki that the present invention produces has enhancing body immunity and effect of slow down aging, and
Edible safety is high, too increases the commodity of Fructus Kaki, make Fructus Kaki have more while improving Quality of persimmon
Attract the specificity of consumer.
(3) SOD Fructus Kaki production method of the present invention is to combine persimmon and the biology of Fructus Kaki self and physiology spy
A kind of SOD Fructus Kaki production method put and work out, the method step is simple, workable, low cost
Honest and clean, effect is notable, while improving Quality of persimmon, the output value of Fructus Kaki is greatly improved, and makes Fructus Kaki industry towards becoming more meticulous
Direction is developed, and reaches highly efficient and productive purpose；The present invention is that Fructus Kaki development provides new thinking, it is indicated that not
Come a new direction of functional Fructus Kaki development, other aspects that this thinking is equally used, such as Folium Kaki
In the production of the product such as tea, Fructus kaki Siccus.
Detailed description of the invention
Below by way of specific embodiment, the present invention is described in further details, but is not limiting as the guarantor of the present invention
Protect scope.
Embodiment 1: use the method to persimmon tree crown spraying fruit tree SOD enzyme preparation to produce SOD Fructus Kaki
Concrete operations are as follows:
Selecting persimmon growing way vigorous, the Cor Bovis seu Bubali Fructus Kaki garden having been enter into production period is tested.Manage at conventional cultivation
Under the conditions of, every mu, every time by fruit tree SOD enzyme preparation 75g, adds 90kg water and dissolves, and stirring mixing is all
After even spraying persimmon tree crown, spray altogether 5 times from squaring period to fruit colour-change period, wherein, squaring period sprays
Mist 1 time, young fruit period sprays 1 time, and fruit expanding period is sprayed 2 times, and fruit colour-change period sprays 1 time, every time between
Every 30-35 days.Each sprinkling should reach the positions such as leaves, trunk, fruit and uniformly adhere to fruit tree SOD enzyme
Formulation soln, typically should spray before at 10 in the morning or after at 3 in afternoon.The Fructus Kaki obtained during results is SOD
Fructus Kaki.
Embodiment 2: use the method to persimmon tree crown spraying fruit tree SOD enzyme preparation to produce SOD Fructus Kaki
Concrete operations are as follows:
Selecting persimmon growing way vigorous, the Cor Bovis seu Bubali Fructus Kaki garden having been enter into production period is tested.Manage at conventional cultivation
Under the conditions of, every mu, every time by fruit tree SOD enzyme preparation 80g, adds 100kg water and dissolves, stirring mixing
After Jun Yun, persimmon tree crown is sprayed, spray altogether from squaring period to fruit colour-change period 5 times, wherein, squaring period
Spraying 1 time, young fruit period is sprayed 1 time, and fruit expanding period is sprayed 2 times, and fruit colour-change period sprays 1 time, every time
It is spaced 30-35 days.Each sprinkling should reach the positions such as leaves, trunk, fruit and uniformly adhere to fruit tree SOD
Enzymes soln, typically should spray before at 10 in the morning or after at 3 in afternoon.The Fructus Kaki obtained during results is
SOD Fructus Kaki.
Embodiment 3: use the method to persimmon tree crown spraying fruit tree SOD enzyme preparation to produce SOD Fructus Kaki
Concrete operations are as follows:
Selecting persimmon growing way vigorous, the Cor Bovis seu Bubali Fructus Kaki garden having been enter into production period is tested.Manage at conventional cultivation
Under the conditions of, every mu, every time by fruit tree SOD enzyme preparation 90g, adds 120kg water and dissolves, stirring mixing
After Jun Yun, persimmon tree crown is sprayed, spray altogether from squaring period to fruit colour-change period 5 times, wherein, squaring period
Spraying 1 time, young fruit period is sprayed 1 time, and fruit expanding period is sprayed 2 times, and fruit colour-change period sprays 1 time, every time
It is spaced 30-35 days.Each sprinkling should reach the positions such as leaves, trunk, fruit and uniformly adhere to fruit tree SOD
Enzymes soln, typically should spray before at 10 in the morning or after at 3 in afternoon.The Fructus Kaki obtained during results is
SOD Fructus Kaki.
Embodiment 4: use the method to persimmon tree crown spraying fruit tree SOD enzyme preparation to produce SOD Fructus Kaki
Concrete operations are as follows:
Selecting persimmon growing way vigorous, the Cor Bovis seu Bubali Fructus Kaki garden having been enter into production period is tested.Manage at conventional cultivation
Under the conditions of, every mu, every time by fruit tree SOD enzyme preparation 100g, adds 140kg water and dissolves, stirring mixing
After Jun Yun, persimmon tree crown is sprayed, spray altogether from squaring period to fruit colour-change period 5 times, wherein, squaring period
Spraying 1 time, young fruit period is sprayed 1 time, and fruit expanding period is sprayed 2 times, and fruit colour-change period sprays 1 time, every time
It is spaced 30-35 days.Each sprinkling should reach the positions such as leaves, trunk, fruit and uniformly adhere to fruit tree SOD
Enzymes soln, typically should spray before at 10 in the morning or after at 3 in afternoon.The Fructus Kaki obtained during results is
SOD Fructus Kaki.
Embodiment 5: use the method to persimmon tree crown spraying fruit tree SOD enzyme preparation to produce SOD Fructus Kaki
Concrete operations are as follows:
Selecting persimmon growing way vigorous, the Cor Bovis seu Bubali Fructus Kaki garden having been enter into production period is tested.Manage at conventional cultivation
Under the conditions of, every mu, every time by fruit tree SOD enzyme preparation 100g, adds 120kg water and dissolves, stirring mixing
After Jun Yun, persimmon tree crown is sprayed, spray altogether from squaring period to fruit colour-change period 5 times, wherein, squaring period
Spraying 1 time, young fruit period is sprayed 1 time, and fruit expanding period is sprayed 2 times, and fruit colour-change period sprays 1 time, every time
It is spaced 30-35 days.Each sprinkling should reach the positions such as leaves, trunk, fruit and uniformly adhere to fruit tree SOD
Enzymes soln, typically should spray before at 10 in the morning or after at 3 in afternoon.The Fructus Kaki obtained during results is
SOD Fructus Kaki.
Embodiment 6: use the method to persimmon trunk injection fruit tree SOD enzyme preparation to produce SOD Fructus Kaki
Selecting persimmon growing way vigorous, the Cor Bovis seu Bubali Fructus Kaki garden having been enter into production period is tested.Concrete operations are as follows:
(1) boring: holing in the region far from ground 10-20cm, the persimmon rhizome portion, drilling hole amount is
3, aperture is 4.5cm, and hole depth reaches heart, and boring and trunk are the angle of 45 degree.
(2) injection: every mu, every time by fruit tree SOD enzyme preparation 90g, adds 3kg water and dissolves, stirring
Injecting in boring after mix homogeneously, inject altogether 5 times from squaring period to fruit colour-change period, wherein, squaring period notes
Penetrating 1 time, young fruit period is injected 1 time, and fruit expanding period is injected 2 times, and fruit colour-change period injects 1 time, every time between
Every 30-35 days.During per injection, the SOD enzyme preparation diluent of the fruit tree needed for individual plant is loaded instillation bag,
Vertically it is hung on the center crotch that about 1.5 meters of ground is high, and the gas in discharge line, then will instil
Syringe needle inserts boring and can inject.
(3) sealing of hole: inject complete, blocks boring with fruit tree wound healing agent, protection boring, promotes more
Close.When fine, injection time is generally 3-14 hour.During injection, fruit tree is by SOD enzyme preparation at least
Within 5-6 hour in advance, dissolve.
Embodiment 7: use the method to persimmon trunk injection fruit tree SOD enzyme preparation to produce SOD Fructus Kaki
Selecting persimmon growing way vigorous, the Cor Bovis seu Bubali Fructus Kaki garden having been enter into production period is tested.Concrete operations are as follows:
(1) boring: holing in the region far from ground 10-20cm, the persimmon rhizome portion, drilling hole amount is
2, aperture is 5.0cm, and hole depth reaches heart, and boring and trunk are the angle of 30 degree.
(2) injection: every mu, every time by fruit tree SOD enzyme preparation 100g, adds 4kg water and dissolves, stir
Inject in boring after mixing mix homogeneously, inject 5 times, wherein, squaring period from squaring period altogether to fruit colour-change period
Injecting 1 time, young fruit period is injected 1 time, and fruit expanding period is injected 2 times, fruit colour-change period injection 1 time, every time
It is spaced 30-35 days.During per injection, the fruit tree needed for individual plant is loaded with SOD enzyme preparation diluent and instils
Bag, is vertically hung on the center crotch that about 1.5 meters of ground is high, and the gas in discharge line, then will
Instillation syringe needle inserts boring and can inject.
(3) sealing of hole: inject complete, blocks boring with dry branch, protection boring, promotes healing.In sky
When gas is sunny, injection time is generally 3-14 hour.During injection, fruit tree at least shifts to an earlier date 5-6 by SOD enzyme preparation
Hour dissolve.
Embodiment 8: use the method to persimmon trunk injection fruit tree SOD enzyme preparation to produce SOD Fructus Kaki
Selecting persimmon growing way vigorous, the Cor Bovis seu Bubali Fructus Kaki garden having been enter into production period is tested.Concrete operations are as follows:
(1) boring: holing in the region far from ground 10-20cm, the persimmon rhizome portion, drilling hole amount is
3, aperture is 5.0cm, and hole depth reaches heart, and boring and trunk are the angle of 40 degree.
(2) injection: every mu, every time by fruit tree SOD enzyme preparation 110g, adds 4kg water and dissolves, stir
Inject in boring after mixing mix homogeneously, inject 5 times, wherein, squaring period from squaring period altogether to fruit colour-change period
Injecting 1 time, young fruit period is injected 1 time, and fruit expanding period is injected 2 times, fruit colour-change period injection 1 time, every time
It is spaced 30-35 days.During per injection, the fruit tree needed for individual plant is loaded with SOD enzyme preparation diluent and instils
Bag, is vertically hung on the center crotch that about 1.5 meters of ground is high, and the gas in discharge line, then will
Instillation syringe needle inserts boring and can inject.
(3) sealing of hole: inject complete, blocks boring with fruit tree wound healing agent, protection boring, promotes more
Close.When fine, injection time is generally 3-14 hour.During injection, fruit tree is by SOD enzyme preparation at least
Within 5-6 hour in advance, dissolve.
Embodiment 9: use the method to persimmon trunk injection fruit tree SOD enzyme preparation to produce SOD Fructus Kaki
Selecting persimmon growing way vigorous, the Cor Bovis seu Bubali Fructus Kaki garden having been enter into production period is tested.Concrete operations are as follows:
(1) boring: holing in the region far from ground 10-20cm, the persimmon rhizome portion, drilling hole amount is
3, aperture is 4.5cm, and hole depth reaches heart, and boring and trunk are the angle of 35 degree.
(2) injection: every mu, every time by fruit tree SOD enzyme preparation 100g, adds 3.5kg water and dissolves, stir
Inject in boring after mixing mix homogeneously, inject 5 times, wherein, squaring period from squaring period altogether to fruit colour-change period
Injecting 1 time, young fruit period is injected 1 time, and fruit expanding period is injected 2 times, fruit colour-change period injection 1 time, every time
It is spaced 30-35 days.During per injection, the fruit tree needed for individual plant is loaded with SOD enzyme preparation diluent and instils
Bag, is vertically hung on the center crotch that about 1.5 meters of ground is high, and the gas in discharge line, then will
Instillation syringe needle inserts boring and can inject.
(3) sealing of hole: inject complete, blocks boring with dry branch, protection boring, promotes healing.In sky
When gas is sunny, injection time is generally 3-14 hour.During injection, fruit tree at least shifts to an earlier date 5-6 by SOD enzyme preparation
Hour dissolve.
Embodiment 10: use tree crown spraying fruit tree SOD enzyme preparation and trunk injection fruit tree SOD enzyme system
The method that agent combines produces SOD Fructus Kaki
Particularly as follows: tree crown spraying fruit tree with the concrete operation method of SOD enzyme preparation with embodiment 1, trunk injection
Penetrate the operational approach of fruit tree SOD enzyme preparation with embodiment 6.
Embodiment 11: use tree crown spraying fruit tree SOD enzyme preparation and trunk injection fruit tree SOD enzyme system
The method that agent combines produces SOD Fructus Kaki
Particularly as follows: tree crown spraying fruit tree with the concrete operation method of SOD enzyme preparation with embodiment 2, trunk injection
Penetrate the operational approach of fruit tree SOD enzyme preparation with embodiment 7.
Embodiment 12: use tree crown spraying fruit tree SOD enzyme preparation and trunk injection fruit tree SOD enzyme system
The method that agent combines produces SOD Fructus Kaki
Particularly as follows: tree crown spraying fruit tree with the concrete operation method of SOD enzyme preparation with embodiment 3, trunk injection
Penetrate the operational approach of fruit tree SOD enzyme preparation with embodiment 8.
Embodiment 13: use tree crown spraying fruit tree SOD enzyme preparation and trunk injection fruit tree SOD enzyme system
The method that agent combines produces SOD Fructus Kaki
Particularly as follows: tree crown spraying fruit tree with the concrete operation method of SOD enzyme preparation with embodiment 4, trunk injection
Penetrate the operational approach of fruit tree SOD enzyme preparation with embodiment 9.
Embodiment 14: use tree crown spraying fruit tree SOD enzyme preparation and trunk injection fruit tree SOD enzyme system
The method that agent combines produces SOD Fructus Kaki
Particularly as follows: tree crown spraying fruit tree with the concrete operation method of SOD enzyme preparation with embodiment 5, trunk injection
Penetrate the operational approach of fruit tree SOD enzyme preparation with embodiment 8.
Embodiment 15: the work of SOD enzyme, soluble solid and Vitamin C content in SOD Fructus Kaki of the present invention
Mensuration
1, the mensuration that SOD enzyme is lived:
(1) pretreatment of material:
Treating excess syndrome example 1-14 produces the SOD Fructus Kaki obtained respectively, common Fructus Kaki (does not spray fruit tree SOD
Enzyme preparation persimmon knot Fructus Kaki) each 0.5g of sarcocarp in the mortar of pre-cooling, add 1ml pre-cooling phosphoric acid
Buffer grinds pulping on ice bath, then adds phosphate buffer and makes the final volume of each sample be 5 milliliters.
Each sample respectively takes 1.2ml centrifugal 20min under 1000rpm, and centrifuged supernatant is SOD crude extract.
(2) SOD enzyme activity determination:
SOD crude extract to each sample carries out SOD enzyme activity determination respectively, and SOD enzyme is lived and used NBT (nitrogen
Blue tetrazolium) photochemical method measure, each sample replication 3 times, whole mensuration process in addition to photochemical reaction,
All carry out under lucifuge or low light condition, do blank with the control tube of not irradiation, measure other respectively and respectively manage
Absorbance.According to formula: (IU/g)=(OD compares-OD sample)/(the 1/2OD comparison) alive of SOD enzyme
× extension rate, can calculate SOD enzyme and live.
2, the mensuration of soluble solid content
Take the Fructus Kaki softened after taking away the puckery taste, carry out homogenized after removing peel and seed, obtain testing sample, so
Rear refractometer is utilized to measure the content of soluble solid in testing sample.
3, the mensuration of Vitamin C content:
(1) pretreatment of material: take the Fructus Kaki softened after taking away the puckery taste, is carried out at homogenate after removing peel and seed
Reason, obtains testing sample, is used for measuring.
(2) reagent: 2% oxalic acid, 2% starch solution, the I2-KI solution of 0.02M, the dimension of 5mg/mL
Raw element C.
(3) method of testing
1), sample treatment: weigh Fructus Kaki pulp 135g, add monoploid and amass 2% oxalic acid homogenate 1min, yarn
Cloth filters, and filtrate is settled to 270ml；
2), titrate: the mensuration of a standard vitamin c solution (5mg/ml), take 2mlVc solution in taper
In Ping, add 1ml starch fluid, be titrated to blueness with iodine liquid and (colour-fast in 30 seconds) occurs, write down iodine liquid
Volume V standard；The ascorbic mensuration of b sample.Take in above-mentioned Fructus Kaki liquid 10ml conical flask, add 1ml
Starch fluid, is titrated to blueness with iodine liquid and (colour-fast in 30 seconds) occurs, write down iodine liquid and amass V sample；
3), 10mg/V standard=M sample (mg)/V sample is calculated；According to Fructus Kaki weight and filtrate volume meter
Calculate the Vc content in every 100g Fructus Kaki.
Table 1 SOD of the present invention Fructus Kaki and the SOD enzyme work in common Fructus Kaki, soluble solid content, dimension
Raw element C content and the comparison of single fruit weight
SOD Fructus Kaki of the present invention contains with the SOD enzyme work in common Fructus Kaki, soluble solid, vitamin C
The comparative result of amount is shown in Table 1.As shown in Table 1, tree crown spraying fruit tree SOD enzyme preparation, trunk injection are used
Penetrate fruit tree SOD enzyme preparation, tree crown sprays the method life combined with trunk injection fruit tree SOD enzyme preparation
In the Fructus Kaki that product obtains, the enzyme of SOD enzyme is lived and is significantly higher than common Fructus Kaki.Wherein, tree crown spraying and trunk are used
In the Fructus Kaki that the method that injection fruit tree combines by SOD enzyme preparation produces, the enzyme of SOD enzyme is lived up to
41IU/g, improves 75.97% than common Fructus Kaki, it can be said that bright, no matter uses tree crown spraying fruit tree to use
SOD enzyme preparation method, trunk injection fruit tree are sprayed with SOD enzyme preparation or tree crown and trunk injection fruit tree is used
The method that SOD enzyme preparation combines may be incorporated for producing the SOD Fructus Kaki with high SOD enzyme activity.This
Outward, soluble solid content in the SOD Fructus Kaki that the present invention produces, Vitamin C content, single fruit weight are the highest
Respectively reached 22.6%, 48.4mg/100g and 202.4g, be respectively increased 10.78% compared with common Fructus Kaki,
7.32% and 9.7%, illustrate that SOD Fructus Kaki production technology can be obviously improved the overall quality of Fructus Kaki.
Claims (4)
1. a SOD Fructus Kaki, it is characterised in that in described SOD Fructus Kaki, the content of SOD is 31-41IU/g.
2. the production method of the SOD Fructus Kaki described in a claim 1, it is characterised in that described production method is
Under conventional cultivation management condition, start squaring period to fruit colour-change period from persimmon, spray fruit tree to persimmon tree crown
By SOD enzyme preparation or/and trunk injection fruit tree SOD enzyme preparation；
The described tree crown spraying fruit tree method of SOD enzyme preparation is: every mu every time by fruit tree SOD enzyme preparation
75-100g, adds 90-140kg water and dissolves, after being uniformly mixed spray persimmon tree crown, from existing
Flower bud phase to fruit colour-change period sprays 5 times altogether, every minor tick 30-35 days；
The described trunk injection fruit tree method of SOD enzyme preparation comprises the following steps:
(1) boring: holing in the region far from ground 10-20cm, the persimmon rhizome portion, drilling hole amount is 2-3
Individual, aperture is 4.5-5.0cm, and hole depth reaches heart, and boring and trunk are the angle of 30-45 degree；
(2) injection: every mu, every time by fruit tree SOD enzyme preparation 90-110g, adds 3-4kg water and dissolves, stir
Injecting in boring after mixing mix homogeneously, inject altogether 5 times from squaring period to fruit colour-change period, per injection is spaced
30-35 days；
(3) sealing of hole: after per injection terminates, blocks boring with fruit tree wound healing agent or dry branch, with protection
Boring, promotes healing.
Production method the most according to claim 2, it is characterised in that described tree crown spraying fruit tree SOD enzyme
The method of preparation is: every mu, every time by fruit tree SOD enzyme preparation 100g, adds 120kg water and dissolves, stir
After mixing mix homogeneously, persimmon tree crown is sprayed, spray altogether 5 times from squaring period to fruit colour-change period, wherein,
Spraying squaring period 1 time, young fruit period is sprayed 1 time, and fruit expanding period is sprayed 2 times, fruit colour-change period spraying 1
Secondary, every minor tick 30-35 days.
Production method the most according to claim 2, it is characterised in that described trunk injection fruit tree SOD enzyme
The method of preparation is: every mu, every time by fruit tree SOD enzyme preparation 110g, adds 4kg water and dissolves, stirring
Injecting in boring after mix homogeneously, inject altogether 5 times from squaring period to fruit colour-change period, wherein, squaring period notes
Penetrating 1 time, young fruit period is injected 1 time, and fruit expanding period is injected 2 times, and fruit colour-change period injects 1 time, every time between
Every 30-35 days.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109548596A (en) * | 2018-12-27 | 2019-04-02 | 长沙谷奥生物科技有限公司 | A kind of high SOD activity corn and its breeding method |
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| CN103385151A (en) * | 2013-08-21 | 2013-11-13 | 葛文爱 | Cultivation method for apples rich in SOD (superoxide dismutase) |
| CN103461057A (en) * | 2013-09-29 | 2013-12-25 | 大连金州后石实业总公司 | Production method for SOD (superoxide dismutase) apples |
| CN105130592A (en) * | 2015-08-05 | 2015-12-09 | 大连岭前农业专业合作社 | Cherry tree nutrition injection liquid |
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| CN103385151A (en) * | 2013-08-21 | 2013-11-13 | 葛文爱 | Cultivation method for apples rich in SOD (superoxide dismutase) |
| CN103461057A (en) * | 2013-09-29 | 2013-12-25 | 大连金州后石实业总公司 | Production method for SOD (superoxide dismutase) apples |
| CN105130592A (en) * | 2015-08-05 | 2015-12-09 | 大连岭前农业专业合作社 | Cherry tree nutrition injection liquid |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN109548596A (en) * | 2018-12-27 | 2019-04-02 | 长沙谷奥生物科技有限公司 | A kind of high SOD activity corn and its breeding method |
| CN109548596B (en) * | 2018-12-27 | 2021-10-08 | 长沙谷奥生物科技有限公司 | Method for cultivating high SOD active corn |
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Application publication date: 20160921 |