CN105940812B - Pentyl xanthate impregnates the ploidy method of mutagenesis of Vitis davidii Foex seed - Google Patents
Pentyl xanthate impregnates the ploidy method of mutagenesis of Vitis davidii Foex seed Download PDFInfo
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- CN105940812B CN105940812B CN201610374957.1A CN201610374957A CN105940812B CN 105940812 B CN105940812 B CN 105940812B CN 201610374957 A CN201610374957 A CN 201610374957A CN 105940812 B CN105940812 B CN 105940812B
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- 244000068653 Vitis davidii Species 0.000 title claims abstract description 68
- 235000004286 Vitis davidii Nutrition 0.000 title claims abstract description 67
- QWENMOXLTHDKDL-UHFFFAOYSA-M pentoxymethanedithioate Chemical compound CCCCCOC([S-])=S QWENMOXLTHDKDL-UHFFFAOYSA-M 0.000 title claims abstract description 63
- 231100000350 mutagenesis Toxicity 0.000 title claims abstract description 26
- 238000000034 method Methods 0.000 title claims abstract description 24
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- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 claims abstract description 28
- 238000007598 dipping method Methods 0.000 claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims description 2
- 238000003756 stirring Methods 0.000 claims 1
- 230000008569 process Effects 0.000 abstract description 6
- 238000001802 infusion Methods 0.000 abstract description 4
- 208000035199 Tetraploidy Diseases 0.000 abstract description 3
- 238000012545 processing Methods 0.000 description 17
- 239000000243 solution Substances 0.000 description 16
- 241000196324 Embryophyta Species 0.000 description 15
- 235000013399 edible fruits Nutrition 0.000 description 12
- IAKHMKGGTNLKSZ-INIZCTEOSA-N (S)-colchicine Chemical compound C1([C@@H](NC(C)=O)CC2)=CC(=O)C(OC)=CC=C1C1=C2C=C(OC)C(OC)=C1OC IAKHMKGGTNLKSZ-INIZCTEOSA-N 0.000 description 10
- 210000000349 chromosome Anatomy 0.000 description 10
- 241000219095 Vitis Species 0.000 description 8
- 235000009754 Vitis X bourquina Nutrition 0.000 description 8
- 235000012333 Vitis X labruscana Nutrition 0.000 description 8
- 235000014787 Vitis vinifera Nutrition 0.000 description 8
- 230000003505 mutagenic effect Effects 0.000 description 7
- 206010028400 Mutagenic effect Diseases 0.000 description 6
- 231100000243 mutagenic effect Toxicity 0.000 description 6
- 229960001338 colchicine Drugs 0.000 description 5
- PHNUZKMIPFFYSO-UHFFFAOYSA-N propyzamide Chemical compound C#CC(C)(C)NC(=O)C1=CC(Cl)=CC(Cl)=C1 PHNUZKMIPFFYSO-UHFFFAOYSA-N 0.000 description 5
- 210000004027 cell Anatomy 0.000 description 4
- 238000005470 impregnation Methods 0.000 description 4
- 239000007787 solid Substances 0.000 description 4
- 239000005602 Propyzamide Substances 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- IBXNCJKFFQIKKY-UHFFFAOYSA-N 1-pentyne Chemical compound CCCC#C IBXNCJKFFQIKKY-UHFFFAOYSA-N 0.000 description 2
- 244000025254 Cannabis sativa Species 0.000 description 2
- 102000029749 Microtubule Human genes 0.000 description 2
- 108091022875 Microtubule Proteins 0.000 description 2
- 208000020584 Polyploidy Diseases 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 150000001412 amines Chemical class 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 210000005069 ears Anatomy 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 229940087559 grape seed Drugs 0.000 description 2
- 230000008595 infiltration Effects 0.000 description 2
- 238000001764 infiltration Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000000386 microscopy Methods 0.000 description 2
- 210000004688 microtubule Anatomy 0.000 description 2
- 229920003229 poly(methyl methacrylate) Polymers 0.000 description 2
- 239000004926 polymethyl methacrylate Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- URCHNZQAFSOGNB-UHFFFAOYSA-N 3-[(3,4-dichlorophenyl)methylamino]cyclopent-2-en-1-one Chemical compound C1=C(Cl)C(Cl)=CC=C1CNC1=CC(=O)CC1 URCHNZQAFSOGNB-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 241000234479 Narcissus Species 0.000 description 1
- 241001464837 Viridiplantae Species 0.000 description 1
- 241000219094 Vitaceae Species 0.000 description 1
- 235000017190 Vitis vinifera subsp sylvestris Nutrition 0.000 description 1
- 244000237969 Vitis vulpina Species 0.000 description 1
- 235000017242 Vitis vulpina Nutrition 0.000 description 1
- FJWGYAHXMCUOOM-QHOUIDNNSA-N [(2s,3r,4s,5r,6r)-2-[(2r,3r,4s,5r,6s)-4,5-dinitrooxy-2-(nitrooxymethyl)-6-[(2r,3r,4s,5r,6s)-4,5,6-trinitrooxy-2-(nitrooxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-3,5-dinitrooxy-6-(nitrooxymethyl)oxan-4-yl] nitrate Chemical compound O([C@@H]1O[C@@H]([C@H]([C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O)O[C@H]1[C@@H]([C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@@H](CO[N+]([O-])=O)O1)O[N+]([O-])=O)CO[N+](=O)[O-])[C@@H]1[C@@H](CO[N+]([O-])=O)O[C@@H](O[N+]([O-])=O)[C@H](O[N+]([O-])=O)[C@H]1O[N+]([O-])=O FJWGYAHXMCUOOM-QHOUIDNNSA-N 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 150000001345 alkine derivatives Chemical class 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
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- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 235000021021 grapes Nutrition 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000002363 herbicidal effect Effects 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 239000010985 leather Substances 0.000 description 1
- 238000002803 maceration Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012067 mathematical method Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 230000007721 medicinal effect Effects 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 239000003471 mutagenic agent Substances 0.000 description 1
- 231100000707 mutagenic chemical Toxicity 0.000 description 1
- 125000000740 n-pentyl group Chemical group [H]C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])* 0.000 description 1
- 229920001220 nitrocellulos Polymers 0.000 description 1
- 229940079938 nitrocellulose Drugs 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
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- 238000000643 oven drying Methods 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
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- 210000001519 tissue Anatomy 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000012991 xanthate Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
- A01H1/08—Methods for producing changes in chromosome number
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Environmental Sciences (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Botany (AREA)
- Developmental Biology & Embryology (AREA)
- Soil Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention discloses a kind of ploidy method of mutagenesis of pentyl xanthate dipping Vitis davidii Foex seed, include the following steps：（1）Pentyl xanthate is dissolved in dimethyl sulfoxide (DMSO), then it is 0.005% 0.007% to be diluted with water to the mass concentration of pentyl xanthate, obtains pentyl xanthate solution；（2）Vitis davidii Foex seed in the stage of showing money or valuables one carries unintentionally is impregnated in pentyl xanthate solution 12 36 hours, that is, completes the ploidy mutagenesis of Vitis davidii Foex seed.The present invention successfully induces Vitis davidii Foex seed to obtain Vitis davidii Foex tetraploid by infusion process, and dipping solution is pentyl xanthate, and most suitable a concentration of 0.006% or so, induced mutation rate is up to more than 60%.
Description
Technical field
The present invention relates to a kind of ploidy method of mutagenesis of pentyl xanthate dipping Vitis davidii Foex seed.
Background technology
Vitis davidii Foex (V.davidii.Foex) belong to East Asia population, 2n=2x=38, be south China main wild grape it
One, the Changjiang river is mainly distributed in the warm and humid rainforest unbroken mountains of south subtropics, can preferably adapt to high temperature and humidity weather conditions, it is normal to grape
Illness evil white rot, anthracnose, anthrachose of grape etc. have fine resistance, and adaptable, this distinct performance is south grape without public affairs
Evilization plantation depicts bright prospects, also provides outstanding resource and material for grape moisture-proof stock and breeding of new variety etc.
Material.The new climing, tender leaf and fruit color of Vitis davidii Foex are very abundant, and appreciation effect and value ratio common glucose higher, are park, front yard
Institute etc. good vertical watches green plants.The color of the leather of most of Vitis davidii Foex fruits is blue black or blue purple, medicinal effects with
The general grape of value ratio is more notable, is more advantageous to people's health.Compared with cultivating other grapes on the south the Changjiang river, Portugal is pierced
Grape are more suitable for the processing and wine brewing of grape, and addition product is more rich, added value higher.Because Vitis davidii Foex is only with above-mentioned several respects
Spy shows, and the peasant in many places wild Vitis davidii Foex man plant and obtains howling success on the south the Changjiang river.
Vitis davidii Foex fruit is smaller, and it is more that fruit includes seed.It is existing using colchicine processing Vitis davidii Foex Germinating Seeds and
Bud is grown, the research for luring its chromosome doubling and successfully obtaining Vitis davidii Foex polyploid is reported.But colchicine is a hypertoxic plant
Alkali, it is careless slightly in operating process, i.e., high risks are brought to process object and operator.
It is now domestic that pentyl xanthate is applied into Vitis davidii Foex and induces the reported success of Vitis davidii Foex chromosome doubling not yet,
The meaning of this research is to act on the Vitis davidii Foex seed sprouted using pentyl xanthate (Propyzamide) for the first time and it is carried out
Ploidy induces, and grain is high-quality greatly to obtain, few seed or the Vitis davidii Foex New MS Polyploid Variety without seed provide excellent germ plasm resource with it is new
Strain.
Invention content
Present invention solves the technical problem that being that pentyl xanthate is applied to the ploidy mutagenesis of Vitis davidii Foex seed, and explore suitable
Suitable ploidy mutagenic condition.
The technical scheme is that a kind of ploidy method of mutagenesis of pentyl xanthate dipping Vitis davidii Foex seed is provided, including
Following steps：
(1) pentyl xanthate is dissolved in dimethyl sulfoxide (DMSO), then is diluted with water to the mass concentration 0.005%- of pentyl xanthate
0.007%, obtain pentyl xanthate solution；
(2) the Vitis davidii Foex seed in the stage of showing money or valuables one carries unintentionally is impregnated in pentyl xanthate solution 12-36 hours, that is, completes thorn Portugal
The ploidy mutagenesis of grape seed.
Further, the mass concentration of dimethyl sulfoxide (DMSO) is 0.04%-0.08% in the pentyl xanthate solution.
Further, the temperature that the Vitis davidii Foex seed is impregnated in pentyl xanthate solution is 24-28 DEG C.
Further, the best dip time of the Vitis davidii Foex seed is 20-28 hours.
Further, the mass concentration of pentyl xanthate is 0.006% in the pentyl xanthate solution.
Further, it during Vitis davidii Foex seed is impregnated in pentyl xanthate solution, shakes or agitation pentyl xanthate is molten
Liquid can improve the oxygen content of solution.
Pentyl xanthate (Propyzamide, CAS：23950-58-5, molecular formula:C12H11Cl2NO；Molecular weight 256.13) again
It is fast enemy's tick；Pronamide；Pentyl xanthate；Propyzamide；Pentyl xanthate standard items；Pentyl xanthate standard solution etc..It, which belongs to, removes
One kind of careless agent has Microtubules in plants albumen very strong binding force, can effectively prevent the polymerization of Microtubules in plants, induces plant dyeing
Body doubles.Compared with colchicine, there is pentyl xanthate hypotoxicity, low concentration, low cost, high efficiency induced chromosome to double
Feature (the current market price is 1g/ members or so).
The present invention is using infusion process, using pentyl xanthate solution treatment Vitis davidii Foex seed, successfully to Vitis davidii Foex seed reality
Existing ploidy mutagenesis.The main innovation of the present invention is：1st, pentyl xanthate solution concentration influences Vitis davidii Foex seeds mutagenesis rate height
Very big, the present invention is by the suitable pentyl xanthate solution concentration that experimental exploring goes out to induce Vitis davidii Foex seed that ploidy variation occurs
0.005%-0.007% (concentration in the present invention refers both to mass concentration), best impregnation concentrations are 0.006%；The dosage and autumn
The dosage of narcissus element is compared, and concentration is more than 100 times low.2nd, the cell point of the Vitis davidii Foex seed in the stage that shows money or valuables one carries unintentionally has been sprouted and has been in
Split it is vigorous, the present invention impregnated in this stage, and pass through the time for reasonably controlling dipping, improve the general of chromosome doubling
Rate, so as to successfully obtain the ploidy variant of Vitis davidii Foex.3rd, dimethyl sulfoxide (DMSO) (DMSO) is done into the faint yellow crystalline powder of pentyl xanthate
The solvent at end, can enhance infiltration and penetration capacity of the pentyl xanthate solution to Vitis davidii Foex seed cell, can be in low pentyne grass
Preferable infiltration is realized under amine aqueous solution concentration conditions to cell tissue, improves chromosome Mutagenic Effect in Vitis davidii Foex seed cell,
Maceration of the Vitis davidii Foex seed by higher drug concentration is avoided, reducing the death rate of seed, (Germinating Seeds are 0.006%
It is impregnated in pentyl xanthate solution for 24 hours, 48%) seed mortality is only.
The invention has the advantages that the present invention successfully impregnates Vitis davidii Foex Germinating Seeds by infusion process to obtain thorn Portugal
Grape tetraploid, dipping solution are pentyl xanthate, most suitable a concentration of 0.006%, induced mutation rate is up to more than 60%；Pentyl xanthate
It is being used in generally making a kind of herbicide in production, and colchicine is a kind of hypertoxic plant extracted from liliaceous plant
Alkali, so no matter in terms of concentration, toxicity, cost, the high low several respects of induced efficiency, it is in plant ploidy induction experiment as can more
Ground substitutes colchicine using pentyl xanthate and makees mutagens, and low concentration, hypotoxicity, low cost, the advantage of high induced efficiency will be non-
Chang Mingxian.
Description of the drawings
Fig. 1 shows Vitis davidii Foex diploid chromosome (2n=2x=38) (× 5500)；
Wherein, "×" represents microscope amplification digit；
Fig. 2 represents Vitis davidii Foex tetraploid chromosomes (2n → 4x=76) (× 5500)；
Fig. 3 represents control (2n) Vitis davidii Foex stomata (× 800)；
Fig. 4 represents variant (4n) Vitis davidii Foex stomata (× 800).
Specific embodiment
With reference to embodiment, the invention will be further described.
Embodiment 1
The ploidy mutagenesis testing designing scheme that pentyl xanthate acts on the Vitis davidii Foex seed sprouted is shown in Table 1：The experiment with
Infusion process carries out.Using clear water dipping as control, if 3 repetitions, totally 12 processing；Often each processing solution is contained in germination box
500ml, Germination effect is roughly the same has sprouted to 100, the Vitis davidii Foex seed in the stage of showing money or valuables one carries unintentionally for dipping, and dip time is respectively
12h、24h、36h.Contents of the DMSO in processing solution as pentyl xanthate organic solvent is 0.04%-0.08%.
Seed immersion test is in 25 DEG C of constant-temperature table (speeds：40∽400rpm；Shimmy amplitude：Φ 25mm) in into
Row.
After the completion of Vitis davidii Foex seed impregnation, seed is cleaned with clear water, is seeded in greenhouse respectively, count survival rate,
Observe Vitis davidii Foex growth and development situation and mutagenic effect.
The ploidy mutagenesis testing design of 1 pentyl xanthate of table dipping Vitis davidii Foex seed
It observes after grape seed is sowed, is carried out with the growth of Vitis davidii Foex plant.
Each processing mortality statistics method：
The death rate=death seed number/sowing seed number (100) × 100%
Each processing induced mutation rate statistical method：
The variation plant number of induced mutation rate=generation/microscopy plant number × 100%.
Leaf morphology and water content observation procedure
It acquires and has stopped growing respectively, there is apparent variation features blade (2n → 4x=76) with compareing blade (2n=2x
=38) each 50, each processing vane thickness is measured with digimatic calipers, is averaged；It is measured everywhere with acrylic glass flat ruler
The length and width of blade are managed, is averaged respectively；Each processing fresh weight (W is weighed with electronic balanceIt is fresh)；Existed using oven drying method
First 70 DEG C in baking oven, latter 105 DEG C each processing blade is dried to constant weight (WIt is dry), using mathematical method calculate variant blade (4n) with
Compare blade (2n) water content：Leaf water content=(WFresh-WIt is dry)/WIt is fresh× 100%.
Stomata observation procedure
Take and stopped growing, had apparent variation features blade (4n) with compareing leaf in the fine day morning 8: 30~10: 00 respectively
Each 10 of piece (2n).A thin layer is applied in blade back master pulse both sides with writing brush libation at an ancient wedding ceremony pyroxylin solution, is taken off after 2~4min with tweezers molten
Glue removes blade back villus.Then one layer is smeared then at master pulse both sides, removes gel die, be placed in micro- Microscopic observation and take pictures.
Complicated and confused rugosity and internode length observation procedure
It chooses respectively and has stopped growing or into slow growth, there are the branches and tendrils (4n) of apparent variation features with compareing branches and tendrils
(2n) each 5 measures the 3rd~12 section (5 × 10=50 sections) internode rugosity, averages respectively；Distinguished with acrylic glass flat ruler
The 3rd~12 section (5 × 10=50 sections) panel length is measured, is averaged.
In fruit with exterior quality observation procedure
On September 15th, 2015, with compareing each 15 fringe of the upper clip fruit ear of branches and tendrils, uses electronics from apparent variation features branches and tendrils
Balance weighs Ear-Weight respectively, calculates 4n and 2n fruit ears and fruit average weight；Distinguished with portable hand-held digital display saccharometer
Said two devices soluble solid content is measured, is averaged.
Interpretation of result
The present embodiment has observed chromosome number while using wall Low Osmotic Method is gone to carry out microscopy to the stem apex of selection
(Fig. 1,2).It is 2n=2x=38 that control Vitis davidii Foex chromosome number is observed in unit field of view, and the dye of Vitis davidii Foex variation type
Colour solid number is mostly 2n → 4x=76, is had a small amount of for types such as 2n=2x=38+4,2n=2x=38+6.
Vitis davidii Foex seed by impregnation is sowed in nutritive cube after 25 days, and the side such as plant ability power is formed to them
It is observed and has been counted in face.As can be seen from Table 2, whether control or various concentration pentyl xanthate processing, with processing when
Between extension, formed plant ability it is on a declining curve, seed mortality is in rising trend.Wherein, it is quilt with the 12nd processing
0.007% pentyl xanthate handles the processing of 36h, death rate highest, up to 91%.
Find out from Fig. 1 and Fig. 2 combinations table 2, Vitis davidii Foex kind has been sprouted with pentyl xanthate various concentration and different time dipping
After son, each processing in addition to control produces ploidy variation plant, and in variation plant body chromosome mostly by 2n=2x
=38 become 2n → 4x=76.Wherein, the processing of Germinating Seeds for 24 hours is impregnated by 0.006% pentyl xanthate with the 8th processing,
Ploidy Mutagenic Effect is best, and ploidy induced mutation rate is up to 63.8%.
The different impregnation concentrations of table 2 and the pentyl xanthate of time are to Vitis davidii Foex seed ploidy Mutagenic Effect
Note：Small letter The English alphabet shows p≤0.05 in table
Pentyl xanthate is to the morphology influence of Vitis davidii Foex blade
50 blade (4n) 50 piece of the acquisition with apparent variation features, control, (2n) blade, from form, size, blade
Thickness etc. is observed and has been analyzed：From in terms of leaf morphology, control Vitis davidii Foex blade (2n) is wealthy avette, by penta
Alkynes grass amine influences the ploidy generated variation blade (4n) for heart；The leaf color of variant blade is more dark green than control, variant
Blade blade face is more crisp and more coarse；Thicker (be shown in Table 3) of the blade than control of ploidy variation is generated；Though control blade is produced than
The blade of raw ploidy variation is long and wide, but is analyzed in terms of the leaf water content height measured, and the blade ratio of ploidy variation occurs
The water content of control is low, and dry matter content is relatively high (being shown in Table 3).
3 Vitis davidii Foex of table compares (2n) compared with ploidy variant (4n) leaf morphology
Note：Small letter The English alphabet shows p≤0.05 in table
Influence of the pentyl xanthate to Stoma of Leaves form and size
It is observed being placed under microscope from variant with compareing the gel die that the blade back of leaf is removed：Variant blade
The length and width of stomata (4n) is all higher than control (2n), illustrates that the stomata of variant blade is bigger than compareing.It sees Fig. 3 and Fig. 4, becomes
The stomatal frequency of allosome blade is smaller than control (see Fig. 3,4).
Influence of the pentyl xanthate to Vitis davidii Foex branches and tendrils rugosity and panel length
From table 4, it can be seen that Vitis davidii Foex becomes the panel length of heterotetraploid compared with compareing diploid, internode significantly becomes
It is short, it is the 54.40% of control；Complicated and confused internode rugosity also significantly attenuates, 50.10% only compareed.This life with autopolyploid
It is related that long rate is generally below diploid.
Influence of 4 pentyl xanthate of table to Vitis davidii Foex branches and tendrils rugosity and panel length
Note：Small letter The English alphabet shows p≤0.05 in table
The change heterotetraploid Vitis davidii Foex fructescence transplanted in the electromechanical Vocationl Technical College's liana garden of Hunan biology with it is right
According to the no significant difference of diploid, the two is all ripe in 8/ last ten-days period~9/ early and middle ten days.Fruit color is all black-and-blue, face after maturation
Color depth is shallow to have no significant difference.As can be seen from Table 6, it is slightly heavy though 4n fruit ears and fruit are all more slightly larger than 2n, it is all not up to notable
It is horizontal.The soluble solid content for becoming heterotetraploid is substantially higher up to 19.39%, 11.15% than control.
Influence of 5 pentyl xanthate of table to fruit shape and soluble solid
Note：Small letter The English alphabet shows p≤0.05 in table
Embodiment 2
Investigate ploidy of the small luffing range of pentyl xanthate concentration with the short time difference of dipping to Vitis davidii Foex Germinating Seeds
Mutagenic Effect：With the pentyl xanthate of 0.0055%, 0.0060%, 0.0065% concentration, impregnate respectively Vitis davidii Foex seed 20h,
For 24 hours, 28h (being shown in Table 6), though its induced mutation rate is all more than 50%, and it is lower than optimum condition (0.006%, for 24 hours) induced mutation rate.
The pentyl xanthate of 6 small luffing concentration of table is with the dipping of short time difference to Vitis davidii Foex seed ploidy Mutagenic Effect
Claims (6)
1. a kind of ploidy method of mutagenesis of pentyl xanthate dipping Vitis davidii Foex seed, which is characterized in that include the following steps：
（1）Pentyl xanthate is dissolved in dimethyl sulfoxide (DMSO), then be diluted with water to pentyl xanthate mass concentration be 0.0055%-
0.0065%, obtain pentyl xanthate solution；
（2）Vitis davidii Foex seed in the stage of showing money or valuables one carries unintentionally is impregnated in pentyl xanthate solution 12-36 hours, that is, completes Vitis davidii Foex kind
The ploidy mutagenesis of son.
2. ploidy method of mutagenesis as described in claim 1, which is characterized in that dimethyl sulfoxide (DMSO) in the pentyl xanthate solution
Mass concentration is 0.04%-0.08%.
3. ploidy method of mutagenesis as described in claim 1, which is characterized in that it is molten that the Vitis davidii Foex seed is impregnated in pentyl xanthate
Temperature in liquid is 24-28 DEG C.
4. ploidy method of mutagenesis as described in claim 1, which is characterized in that the dip time of the Vitis davidii Foex seed is 20-
28 hours.
5. ploidy method of mutagenesis as described in claim 1, which is characterized in that pentyl xanthate quality in the pentyl xanthate solution
A concentration of 0.006%.
6. ploidy method of mutagenesis as described in claim 1, which is characterized in that be impregnated in pentyl xanthate solution in Vitis davidii Foex seed
During, shake or stir pentyl xanthate solution.
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| CN104488696A (en) * | 2014-12-15 | 2015-04-08 | 广西大学 | Breeding method for kumquat triploid |
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| CN104488696A (en) * | 2014-12-15 | 2015-04-08 | 广西大学 | Breeding method for kumquat triploid |
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| 化学药剂诱导植物多倍体研究进展;黄权军;《安徽农学通报》;20091231;第15卷(第5期);第58-59页 * |
| 秋水仙素对刺葡萄萌发种子诱变的影响;石雪晖等;《中外葡萄与葡萄酒》;20071231(第5期);第7-9页 * |
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