CN105935439B - Traditional Chinese medicine with effects of warming lung and descending turbidity and preparation method and application thereof - Google Patents
Traditional Chinese medicine with effects of warming lung and descending turbidity and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine with the efficacy of warming lung and descending turbidity, which is mainly prepared from the following raw material medicines: prepared aconite root, pilose asiabell root, dried ginger, rhubarb horsetails, notoginseng, honey-fried licorice root. The meridian tropism of all the medicines listed in the formula is basically directly related to the lung and the large intestine, and the combination of all the medicines has better functions of warming the lung and descending the turbid. The compound preparation has obvious effects on promoting the survival, growth and development and differentiation of nerve cells, delaying the natural death of the nerve cells, participating in the aspect of cerebral ischemia injury protection mechanism and the like, has biological effects of preventing the death of the nerve cells after being damaged, improving the pathological state of the nerve cells, promoting the regeneration and differentiation of the damaged nerve cells and the like, is used for treating vascular dementia, can promote the recovery of the nerve cells and improve the dementia symptom.
Description
Technical Field
The invention belongs to the field of traditional Chinese medicines, and particularly relates to a traditional Chinese medicine with effects of warming lung and descending turbidity, and a preparation method and application thereof.
Background
Modern medicine considers that vascular dementia (VaD) is a clinical syndrome mainly manifested by intellectual impairment and cognitive dysfunction due to the damage of brain tissue caused by various cerebrovascular diseases (such as cerebral hemorrhage, cerebral infarction, etc.), and the clinical syndrome mainly manifested by learning, memory and cognitive dysfunction mainly comprises the decline of learning and memory. The occurrence of vascular dementia is closely related to the apoptosis and necrosis of brain nerve cells.
With the enhancement of health care consciousness of people, the Chinese herbal compound preparation aiming at the disease characteristics of vascular dementia is produced, and the Chinese herbal compound preparation has increasingly attracted attention by people with the advantages of nature, safety, no toxic or side effect and the like. Therefore, the development of the traditional Chinese medicine compound preparation with the traditional Chinese medicine characteristic for preventing and treating the vascular dementia can not only make up for the deficiency in modern medical treatment and exert the advantages of the traditional Chinese medicine, but also is suitable for wide crowds, has large market demand and has wide market prospect.
Disclosure of Invention
The invention aims to solve the technical problem of providing a traditional Chinese medicine with the effects of warming lung and reducing turbidity, and a preparation method and application thereof.
In order to solve the technical problems, the invention adopts the following technical scheme: the traditional Chinese medicine with the effects of warming lung and descending turbidity is mainly prepared from the following raw material medicines: prepared aconite root, pilose asiabell root, dried ginger, rhubarb horsetails, notoginseng, honey-fried licorice root.
The traditional Chinese medicine with the effects of warming lung and descending turbidity is prepared from the following raw medicinal materials in parts by weight: 10-30 parts of prepared monkshood, 5-25 parts of codonopsis pilosula, 5-15 parts of dried ginger, 5-15 parts of wine-processed rhubarb, 5-15 parts of pseudo-ginseng and 2-10 parts of honey-fried licorice root.
The traditional Chinese medicine with the effects of warming lung and descending turbidity is prepared from the following raw medicinal materials in parts by weight: 20 parts of prepared monkshood, 15 parts of codonopsis pilosula, 10 parts of dried ginger, 10 parts of wine-processed rhubarb, 10 parts of pseudo-ginseng and 6 parts of honey-fried licorice root.
The application of the traditional Chinese medicine with the effects of warming lung and descending turbidity in preparing the medicine for treating vascular dementia.
The preparation method of the traditional Chinese medicine with the efficacy of warming lung and reducing turbidity comprises the steps of extracting 5 traditional Chinese medicines of codonopsis pilosula, dried ginger, wine-treated rhubarb, pseudo-ginseng and honey-fried licorice root by an ethanol reflux method; extracting radix Aconiti lateralis Preparata by percolation.
The ethanol reflux method comprises the following steps of; extracting radix Codonopsis, Zingiberis rhizoma, radix et rhizoma Rhei, Notoginseng radix, and radix Glycyrrhizae Preparata with 60% ethanol for 3 times (8-10 times), mixing extractive solutions, and concentrating; extracting the residues of each single medicinal material with water in an amount of 8-10 times for 3 times, mixing the extractive solutions, and concentrating; mixing the ethanol extract and the water extract, and drying at 60 deg.C to obtain the total extract of each single medicinal material.
The ethanol reflux method comprises the following steps: respectively weighing 500g of each unit of medicinal materials, performing reflux extraction for 3 times by using 5000ml of 60% ethanol as a reflux extraction solvent according to the liquid-material ratio of 10:1, wherein the reflux extraction temperature is 100 ℃, the reflux extraction time is 1.5 hours each time, combining the extracting solutions, and concentrating to the volume of 1/3 of the crude drugs; volatilizing ethanol from the residues after ethanol extraction, decocting the residues with 5000ml of distilled water according to the liquid-material ratio of 10:1 for 3 times with 1 hour each time, mixing the decoction, and concentrating to crude drug volume; mixing the ethanol extractive solution and the water extractive solution, drying at 60 deg.C to obtain total extracts of the single medicinal materials, and weighing to calculate the extraction rate.
The percolation method comprises the following processes: soaking radix Aconiti lateralis Preparata at a liquid-to-material ratio of 10:1 with water, percolating, mixing extractive solutions, concentrating, and drying at 60 deg.C to obtain radix Aconiti lateralis Preparata extract.
The percolation method is carried out according to the following steps: weighing 500g of radix aconiti lateralis preparata medicinal material, adding 5000ml of water cooling infiltration percolation extraction according to the liquid-material ratio of 10:1, merging the extracting solution, concentrating, drying at 60 ℃ to obtain the radix aconiti lateralis preparata extract, weighing and calculating the extraction rate.
The preparation method comprises mixing the ethanol reflux extract and the percolation extract with pharmaceutical adjuvants according to formula ratio, and making into preparation.
The inventor scientifically applies traditional Chinese medicine dialectical treatment on the basis of combining macroscopic dialectical analysis and microscopic dialectical analysis, and develops a traditional Chinese medicine with the effects of warming lung and reducing turbidity by adopting a lung-warming and turbidity-reducing method aiming at the pathological mechanism of lung qi deficiency and turbid toxin accumulation in intestinal tracts in the occurrence, development and evolution processes of vascular dementia, wherein the traditional Chinese medicine is mainly prepared from the following raw material medicines: prepared aconite root, pilose asiabell root, dried ginger, rhubarb horsetails, notoginseng, honey-fried licorice root. The meridian tropism of all the medicines listed in the formula is basically directly related to the lung and the large intestine, and the combination of all the medicines has better functions of warming the lung and descending the turbid.
The Fu Zi warms kidney and is the key herb for passing twelve meridians and pure yang. It can warm kidney water greatly, make fire exuberant and water boil, refine into qi, and is the monarch drug. Codonopsis pilosula, radix Codonopsis is sweet and neutral in nature and enters spleen and lung meridians. Ben Cao gang mu Shi Yi (supplement to compendium of materia Medica) is characterized in that: the codonopsis pilosula is used for treating lung deficiency and tonifying lung qi, so the lung is tonified by the codonopsis pilosula, the lung qi is abundant, the kidney is helped to store essence and generate marrow, the spleen is helped to disperse essence and qi, the heart is helped to nourish qi and transport blood vessels, and the liver is helped to soften tendons and dredge, so the codonopsis pilosula is a ministerial drug. Gan Jiang is pungent in flavor and hot in property, has the actions of warming lung, warming middle energizer to dispel cold, returning yang to unblock pulse, and excels in warming and dispersing lung meridian cold. Although Fu Zi and gan Jiang are pungent and hot in nature, they have different properties, so Fu Zi can move but not keep, and gan Jiang can move but not keep. The two herbs are matched, one is walking and the other is on the right, and the two herbs warm yang and nourish lung qi together. Tianqi is sweet and warm in nature, slightly bitter, entering lung and stomach meridians. It is especially good at activating blood and resolving stasis, and has the characteristic of resolving stasis without damaging healthy qi. It is indicated for blood stasis due to the disorder of dispersing and descending of lung qi. Da Huang is powerful and easy to move, called general, and enters large intestine mainly to purge and relax bowels, wash out the accumulated food in stomach and intestine and remove the excessive heat, so it is the essential herb for constipation and accumulated food in stomach and intestine. So it is said from Shen nong Ben Cao Jing that it has the actions of removing blood stasis, breaking accumulation, cleaning intestines and stomach, promoting the growth of old herb, smoothing water and smoothing five internal organs. The work of "" is done. Prepared licorice root, radix Glycyrrhizae Praeparata, being sweet and warm in nature, enters lung and stomach meridians and has the effect of tonifying middle-jiao and relieving urgency. It can tonify spleen and qi, moisten lung and check adverse rise of qi, and alleviate heat and drastic herbs, so it is mild in nature and does not hurt healthy qi.
The compound preparation has obvious effects on promoting the survival, growth and development and differentiation of nerve cells, delaying the natural death of the nerve cells, participating in the aspect of cerebral ischemia injury protection mechanism and the like, has biological effects of preventing the death of the nerve cells after being damaged, improving the pathological state of the nerve cells, promoting the regeneration and differentiation of the damaged nerve cells and the like, is used for treating vascular dementia, can promote the recovery of the nerve cells and improve the dementia symptom.
Drawings
FIG. 1 is a process flow chart of the preparation method of the traditional Chinese medicine (granule) with the effects of warming lung and descending turbidity.
Detailed Description
Research process of traditional Chinese medicine with lung warming and turbidity descending effects
First, the formula composition
According to the weight portion, 20 portions of prepared aconite, 15 portions of radix codonopsitis, 10 portions of dried ginger, 10 portions of wine rhubarb, 10 portions of pseudo-ginseng and 6 portions of honey-fried licorice root.
The characteristics of the raw medicinal materials are as follows:
preparing monkshood: the product is processed product of radix Aconiti Kusnezoffii of Ranunculaceae. The product is as follows: pungent, sweet and big heat; is toxic. It enters heart, kidney and spleen meridians. It is called "radix Aconiti lateralis Preparata" for reducing toxicity after processing by salting, rinsing with water, decocting, and parching. The functions are as follows: reviving yang to rescue adverse qi, tonifying fire to strengthen yang, and expelling pathogenic wind, cold and dampness. The main components are monoester alkaloids with less toxicity: benzoylaconitine, benzoylmesaconine, benzoylhypaconine, even hydrolyzed to the less toxic aminoalcohol bases: aconitine, mesaconitine, hypaconitine, etc. The pharmacological actions of the compound include arrhythmia resistance, shock resistance, myocardial protection, inflammation resistance, pain relief and the like.
Codonopsis pilosula: the product is a perennial herb of Codonopsis genus of Campanulaceae family. The product is as follows: sweet and neutral, enter spleen and lung meridians. The efficacy is as follows: tonify middle-jiao and Qi, promote the production of body fluid and nourish blood. Radix Codonopsis contains various saccharides, phenols, sterols, volatile oil, baicalein glucoside, saponin, and trace alkaloids. The pharmacological action is wide: ginseng can regulate the mutual balance of excitation and inhibition processes of the central nervous system, and has the facilitation effects of different degrees on the acquisition, consolidation and reproduction of memory. Ginsenoside has stimulating effect on pituitary-adrenal cortex system, can improve stress state of organism, and has effects of resisting tissue ischemia, anoxia, shock, oxidation, cell mutation, apoptosis, necrosis, bidirectionally regulating blood pressure and blood sugar, inhibiting platelet aggregation, reducing blood lipid, resisting atherosclerosis, protecting and stimulating bone marrow hemopoiesis, and prolonging life.
Dried ginger: is dried rhizome of Zingiber officinale Roscoe of Zingiberaceae. Pungent flavor and warm property. It enters spleen, stomach, heart and lung meridians. The efficacy is as follows: warming the middle energizer to dispel cold, restoring yang to activate collaterals, drying dampness and dissolving phlegm, warming the lung to resolve retained fluid. The dried ginger contains 2-3% of volatile oil, which is light yellow or yellowish green oily liquid, and the oil contains zingiberone as the main component, and beta-bisabolene, alpha-curcumene and the like as the secondary components. Modern pharmacological actions: exciting blood vessel movement center and heart respiration center, exciting heart, resisting histamine, inhibiting bacteria, and inhibiting platelet aggregation.
Wine-processed rhubarb: is dried rhizome of Polygonum palmatum (L.) Druce of Polygonaceae. This herb is bitter in flavor, cold in nature and settled. It enters spleen, stomach, large intestine, liver and heart meridians. The rhubarb slices are sprayed with yellow wine and stirred evenly, put into a pot for slight frying, taken out and dried to obtain the rhubarb wine. The functions of purgation and accumulation elimination, clearing heat and fire, stopping bleeding, detoxifying, activating blood and dissolving stasis. Mainly contains anthraquinone derivatives, and the main components are physcion 1-glucoside, tannin, organic acid, etc. The pharmacological actions of the Chinese medicinal composition have the effects of promoting intestinal peristalsis, promoting defecation, diminishing inflammation, resisting infection, resisting thrombosis, benefiting gallbladder, protecting liver, reducing blood pressure, stopping bleeding, reducing cholesterol and the like.
Pseudo-ginseng: is dried root of Araliaceae perennial herb Notoginseng radix. Sweet, slightly bitter and warm in nature. It enters liver and stomach meridians. Has the functions of removing blood stasis and stopping bleeding, and relieving swelling and pain, has the characteristics of stopping bleeding without retaining blood stasis and removing blood stasis without damaging healthy qi, and is a good medicine for blood syndrome and a key medicine for traumatology. The medicine is widely used for angina pectoris of coronary heart disease, cerebral apoplexy sequela and the like, and has satisfactory curative effect. The product mainly contains tetracyclic triterpenoid saponin active ingredients, has remarkable hemostasis effect, and can shorten blood coagulation time; the anticoagulant function is realized, the fibrinolysis is promoted, and the viscosity of the whole blood is reduced; it also has effects in dilating cerebral vessels, increasing cerebral blood flow, regulating sugar metabolism, and resisting aging.
Honey-fried licorice root: is a plant of Leguminosae, and radix Glycyrrhizae Preparata is honey-baked radix Glycyrrhizae. Sweet in nature and mild in nature. It enters heart, spleen, lung and stomach meridians. The efficacy is as follows: invigorating spleen and replenishing qi, moistening lung for arresting cough, clearing heat and detoxicating, relieving pain, and alleviating drug property. Mainly contains glycyrrhizic acid, liquiritin, etc. The pharmacological action of the compound can resist arrhythmia, reduce fat, reduce cholesterol, resist platelet aggregation and the like.
Second, process screening
1. Preliminary process route
The inventor starts with the chemical components of each medicinal material in the formula, seriously considers the internal relationship between the chemical components of each medicinal material and the clinical curative effect, and draws a large number of documents to draw up the following process route according to the properties and the pharmacological action of the chemical components of each medicinal material:
process I: reflux-extracting six (radix Aconiti lateralis Preparata, radix Codonopsis, Zingiberis rhizoma, radix et rhizoma Rhei, Notoginseng radix, and radix Glycyrrhizae Preparata) medicinal materials with 85% ethanol for three times, each time adding 8 times of ethanol, recovering ethanol under reduced pressure, concentrating the extractive solution to obtain soft extract, drying, and pulverizing.
And (2) a process II: adding 8 times of water into 6 medicinal materials, decocting for three times, decocting for 2 hours each time, mixing filtrates, concentrating, adding ethanol for precipitating, recovering ethanol under reduced pressure, concentrating, drying, and pulverizing for use.
Process III: extracting 5 traditional Chinese medicines of codonopsis pilosula, dried ginger, wine-processed rhubarb, panax notoginseng and honey-fried licorice root by adopting an ethanol reflux method: extracting with 8-10 times of 60% ethanol for 3 times, mixing extractive solutions, concentrating, extracting with 8-10 times of water for 3 times, and drying at 60 deg.C. Extracting the prepared monkshood by a percolation method: hot soaking radix Aconiti lateralis Preparata in water, percolating to extract at ratio of 10:1, mixing extractive solutions, concentrating, and drying at 60 deg.C.
2. Pharmacological screening
40 Kunming mice, the male and female are not limited, and the test sample is extract powder prepared according to the three processes. Each group contains 10 control groups (no administration), the second, third and fourth groups respectively comprise Process I, Process II and Process III (administration dosage is 1ml/100g body weight, and is about 10 times of human clinical daily dosage), continuous gavage administration is carried out for 7 days, 2-V0 method is adopted to prepare Vascular Dementia (VD) rat model, Morris water maze is adopted to test learning and memory ability of the rat (behavioural test), and the results are as follows.
(1) Positioning navigation experiment results: with the increase of training days, the escape latency of rats in each group is gradually shortened, wherein the process III group is the most obvious; the escape latency of the process group III is significantly reduced (P < 0.05) on days 4 and 5 compared with the control group. (see Table 1).
(2) The results of the space search test are: compared with the control group, the times of crossing the platform and the time of staying in the platform quadrant of the process III medicament group are obviously increased, and the difference has statistical significance (P is less than 0.05) (see table 1).
TABLE 1 VD rat Morris Water maze test results statistical comparison (
n＝10)
Note: compared with the control group, the compound of the formula,#P＜0.05。
the results show that the process III can obviously improve the behavioral function compared with a control group, and finally confirm that the process route III is adopted, and the specific steps are as follows;
the ethanol reflux method comprises the following steps: 500g of each of 5 traditional Chinese medicine materials of codonopsis pilosula, dried ginger, wine-processed rhubarb, pseudo-ginseng and honey-fried licorice root are weighed respectively. Reflux-extracting with 5000ml 60% ethanol at a ratio of 10:1 at 100 deg.C for 1.5 hr for 3 times, mixing extractive solutions, and concentrating to 1/3 volume of crude drug; volatilizing ethanol from the residues after ethanol extraction, decocting the residues with 5000ml of distilled water according to the liquid-material ratio of 10:1 for 3 times with 1 hour each time, mixing the decoction, and concentrating to crude drug volume; mixing the ethanol extractive solution and the water extractive solution, drying at 60 deg.C to obtain total extracts of the single medicinal materials, and weighing to calculate the extraction rate.
The percolation method is carried out according to the following steps: weighing 500g of prepared monkshood, adding 5000ml of water cooling infiltration percolation extraction according to the liquid-material ratio of 10:1, merging the extracting solution, concentrating, drying at 60 ℃ to obtain the prepared monkshood extract, weighing and calculating the extraction rate.
The preparation method comprises the following steps of calculating the content of the extract according to the dosage of each medicine in the prescription, and adding auxiliary materials including dextrin (1: 1, the mass ratio of medicinal materials to auxiliary materials), sucrose (1: 0.3), magnesium stearate (1: 0.02) and 10% ethanol solution to prepare bagged granules, wherein the main preparation steps comprise that ① sieving is performed, the extract is crushed and sieved by a 120-mesh sieve, the dextrin and the sucrose are respectively sieved by a 100-mesh sieve, ② mixing materials are performed, the main medicine and the auxiliary materials are uniformly mixed by sieving by an 80-mesh sieve, preparing soft materials, ③ granulating is performed, a proper amount of 10% ethanol solution is added into the mixing materials to prepare soft materials, sieving by an 18-mesh sieve, granulating by drying by ④, drying wet granules at 50-60 ℃, ⑤ drying is performed, dry granules are sieved by a 16-mesh sieve, adding magnesium stearate according to the formula amount, uniformly mixing, and packaging by a ⑥, and 10g of magnesium stearate is packaged per bag.
Third, study of pharmaceutical analysis
Through the previous clinical research, the prescription is determined as follows: 20g of prepared monkshood, 15g of codonopsis pilosula, 10g of dried ginger, 10g of wine-processed rhubarb, 10g of pseudo-ginseng and 6g of honey-fried licorice root. Three batches of lung-warming and turbidity-reducing granule samples are prepared according to the determined process line of the second step for clinical research. And according to the relevant national regulations, three batch number samples are subjected to an accelerated stability test and a normal temperature sample retention stability test. The result shows that the stability of the preparation process formula meets the requirement.
Wherein the content of the first and second substances,
determination of contents of chemical components
Weighing a certain amount of compound medicinal material extract, and determining the contents of aconitine as main ingredient in radix Aconiti lateralis Preparata, n-butanol as main ingredient in radix Codonopsis, zingiberone as main ingredient in Zingiberis rhizoma, rhein as main ingredient in wine radix et rhizoma Rhei, sanchinoside R1 as main ingredient in Notoginseng radix, and glycyrrhizin as main ingredient in radix Glycyrrhizae Preparata by High Performance Liquid Chromatography (HPLC); and measuring the content of polysaccharide in the codonopsis pilosula by using a sulfuric acid-phenol colorimetric method.
Compound fingerprint detection
Accurately weighing a certain amount of aconitine, radix codonopsitis n butanol, zingiberenone and notoginsenoside R1 reference substances, placing the reference substances in a 50ml brown volumetric flask, adding 70% methanol for dissolving and diluting to a scale, accurately weighing 3-5ml, placing the reference substances in a 50ml volumetric flask, adding 70% methanol for diluting to the scale, shaking up, and scanning under the wavelength of 800nm of ultraviolet 200-. The HPLC absorption wavelength was determined by uv scanning of the three components. Because the compound consists of fat-soluble components and water-soluble components, and the water-soluble active components are polysaccharides, the water-soluble components are qualitatively and quantitatively controlled by a sulfuric acid phenol method. Taking five batches of compound granule samples, respectively processing according to the preparation method of the test sample, and determining the chromatogram. And calculating the content average number and RSD value of index components of aconitine, radix Codonopsis n-butanol, zingiberone and notoginsenoside R1 according to the peak area. The quality standard of the medicine meeting the requirement of new medicine declaration is worked out, and the quality standard of the medicine with advanced standard, strong specificity and high technical content is strived to be achieved so as to control the quality of the medicine.
Fourth, clinical application
1. Old man, 52 years old, originally diagnosed as cerebral infarction, leave after western medicine treatment lethargy, lose consciousness, amnesia, salivation, anorexia, difficult constipation, etc., the tongue is dull, there is little coating, deep and thready pulse is developed for more than one year, many sides seek to treat and do not see obvious improvement, in order to ask Chinese medicine treatment, come to visit to our hospital, give the aforesaid warm lung and reduce turbidity granule 3 times a day, 1 bag each time, soak into 100ml, each 1 time in the morning, evening, after taking 6 courses of treatment with water, patient's spirit is clear, does not have lethargy, can exchange with family's simplicity, stool improvement.
2. The patients who are yellow and have 65 years old are in the women, the patients with hypertension have more than 20 years old, and the patients who are admitted to the hospital in 2013 have lost of consciousness, hypomnesis, amnesia, apathy and the like after active treatment, the tongue is dull, the tongue coating is white, and the pulse is deep and thin. After half a year of disease onset, the symptoms are aggravated, and the clinic visit of the hospital is followed, the lung-warming and turbidity-reducing granules are prepared into 100ml after being brewed into 1 package every 3 times and 1 time respectively in the morning, noon and evening, and the granules are taken with warm water, after 5 treatment courses, the patients lose their mind better than before, the communication can be simple, and the memory can be recovered. 3. Li Yi, woman, 58 years old, original diagnosis cerebral infarction, hypertension, diabetes, right side limb hemiplegia when in illness, limb hemiplegia better than before after hospitalization but leave behind symptoms such as memory, computing power decline, intelligence decline, speech unclear, expression indifference, tongue dull, white fur, deep and thready pulse, constipation etc., give the aforesaid lung-warming and turbidity-lowering granule 3 times a day, 1 bag each time, steep into 100ml, each 1 time in the morning, evening, after taking 10 courses of treatment with water, intelligence revives, thinking ability tends to normal.
Claims (6)
1. A traditional Chinese medicine with effects of warming lung and descending turbidity is characterized by being prepared from the following raw medicinal materials in parts by weight: 10-30 parts of prepared monkshood, 5-25 parts of codonopsis pilosula, 5-15 parts of dried ginger, 5-15 parts of wine-processed rhubarb, 5-15 parts of pseudo-ginseng and 2-10 parts of honey-fried licorice root; the specific preparation method comprises the following steps: extracting 5 traditional Chinese medicines of codonopsis pilosula, dried ginger, wine-processed rhubarb, panax notoginseng and honey-fried licorice root by adopting an ethanol reflux method; the ethanol reflux process comprises the following steps: extracting radix Codonopsis, Zingiberis rhizoma, radix et rhizoma Rhei, Notoginseng radix, and radix Glycyrrhizae Preparata with 60% ethanol for 3 times (8-10 times), mixing extractive solutions, and concentrating; extracting the residues of each single medicinal material with water in an amount of 8-10 times for 3 times, mixing the extractive solutions, and concentrating; mixing the ethanol extract and the water extract, and drying at 60 deg.C to obtain total extract of each single medicinal material; extracting radix Aconiti lateralis Preparata by percolation; the percolation method comprises the following processes: soaking radix Aconiti lateralis Preparata at a liquid-to-material ratio of 10:1 with water, percolating, mixing extractive solutions, concentrating, and drying at 60 deg.C to obtain radix Aconiti lateralis Preparata extract; mixing the ethanol reflux extract and the percolation extract with pharmaceutical adjuvants according to formula ratio, and making into preparation.
2. The traditional Chinese medicine with the effect of warming lung and descending turbidity according to claim 1, which is characterized by being prepared from the following raw medicinal materials in parts by weight: 20 parts of prepared monkshood, 15 parts of codonopsis pilosula, 10 parts of dried ginger, 10 parts of wine-processed rhubarb, 10 parts of pseudo-ginseng and 6 parts of honey-fried licorice root.
3. The use of the Chinese medicament with lung warming and turbid pathogen descending effects of claim 1 in the preparation of medicaments for treating vascular dementia.
4. The preparation method of the traditional Chinese medicine with the efficacy of warming lung and descending turbidity according to claim 1, which is characterized in that: extracting 5 traditional Chinese medicines of codonopsis pilosula, dried ginger, wine-processed rhubarb, panax notoginseng and honey-fried licorice root by adopting an ethanol reflux method; the ethanol reflux process comprises the following steps: extracting radix Codonopsis, Zingiberis rhizoma, radix et rhizoma Rhei, Notoginseng radix, and radix Glycyrrhizae Preparata with 60% ethanol for 3 times (8-10 times), mixing extractive solutions, and concentrating; extracting the residues of each single medicinal material with water in an amount of 8-10 times for 3 times, mixing the extractive solutions, and concentrating; mixing the ethanol extract and the water extract, and drying at 60 deg.C to obtain total extract of each single medicinal material; extracting radix Aconiti lateralis Preparata by percolation; the percolation method comprises the following processes: soaking radix Aconiti lateralis Preparata at a liquid-to-material ratio of 10:1 with water, percolating, mixing extractive solutions, concentrating, and drying at 60 deg.C to obtain radix Aconiti lateralis Preparata extract; mixing the ethanol reflux extract and the percolation extract with pharmaceutical adjuvants according to formula ratio, and making into preparation.
5. The method according to claim 4, characterized by the following steps: respectively weighing 500g of each unit of medicinal materials, performing reflux extraction for 3 times by using 5000ml of 60% ethanol as a reflux extraction solvent according to a liquid-material ratio of 10:1, wherein the reflux extraction temperature is 100 ℃, the reflux extraction time is 1.5 hours each time, combining the extracting solutions, and concentrating to 1/3 of the volume of the crude drug; volatilizing ethanol from the residues after ethanol extraction, decocting the residues with 5000ml of distilled water according to the liquid-material ratio of 10:1 for 3 times with 1 hour each time, combining the water decoction, and concentrating to crude drug volume; mixing the ethanol extractive solution and the water extractive solution, drying at 60 deg.C to obtain total extracts of the single medicinal materials, and weighing to calculate the extraction rate.
6. The method according to claim 5, characterized by the following steps: weighing 500g of prepared monkshood medicinal material, adding 5000ml of water cooling infiltration percolation extraction according to the liquid-material ratio of 10: l, combining the extracting solutions, concentrating, drying at 60 ℃ to obtain prepared monkshood extract, weighing and calculating the extraction rate.
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| CN104645015A (en) * | 2015-01-27 | 2015-05-27 | 广西中医药大学第一附属医院 | Traditional Chinese medicine compound preparation for treating vascular dementia and preparation method of traditional Chinese medicine compound preparation |
| CN104740305A (en) * | 2015-04-10 | 2015-07-01 | 福建省第二人民医院 | Prescription with effects of nourishing kidney and reducing turbidity and preparation method thereof |
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| CN104645015A (en) * | 2015-01-27 | 2015-05-27 | 广西中医药大学第一附属医院 | Traditional Chinese medicine compound preparation for treating vascular dementia and preparation method of traditional Chinese medicine compound preparation |
| CN104740305A (en) * | 2015-04-10 | 2015-07-01 | 福建省第二人民医院 | Prescription with effects of nourishing kidney and reducing turbidity and preparation method thereof |
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| 唐农教授从肺论治血管性痴呆的经验;赵清山等;《贵阳中医学院学报》;20140131;第36卷(第1期);第6-8页 * |
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