CN105918505B - One kind protecting cardiovascular ready-mixed oil rich in alpha-linolenic acid - Google Patents

One kind protecting cardiovascular ready-mixed oil rich in alpha-linolenic acid Download PDF

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CN105918505B
CN105918505B CN201610447650.XA CN201610447650A CN105918505B CN 105918505 B CN105918505 B CN 105918505B CN 201610447650 A CN201610447650 A CN 201610447650A CN 105918505 B CN105918505 B CN 105918505B
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fatty acid
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acid
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CN105918505A (en
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梁水永
陈继承
梁跃坪
陈玉婷
龚晓芳
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Xiamen Tekang Agricultural Biotechnology Co Ltd
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    • AHUMAN NECESSITIES
    • A23FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23DEDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
    • A23D9/00Other edible oils or fats, e.g. shortenings, cooking oils
    • A23D9/02Other edible oils or fats, e.g. shortenings, cooking oils characterised by the production or working-up
    • A23D9/04Working-up

Abstract

The invention discloses one kind to protect cardiovascular ready-mixed oil rich in alpha-linolenic acid, is deployed by five kinds or five kinds or more in linseed oil, eucommia ulmoides seed oil, peony seed oil, Purple Perilla Seed Oil, walnut oil, tomato seed oil, safflower seed oil, acer truncatum seed oil, black sesame oil.Wherein the content of alpha-linolenic acid reaches 20%-48%, and alpha-linolenic acid can be converted into EPA, DHA of needed by human body, and saturated fatty acid total amount less than 10%, coordinate by various fatty acid composition ratio, meets diet nutritional guide recommended amounts.Feedstock oil is that cold press is obtained through physical refining, does not add any chemical synthesis antioxidant, and the active constituent rich in a large amount of protection cardiovascular and cerebrovasculars has and significantly protects cardiovascular function.

Description

One kind protecting cardiovascular ready-mixed oil rich in alpha-linolenic acid
Technical field
The invention belongs to edible oil technical fields, and in particular to one kind protects cardiovascular ready-mixed oil rich in alpha-linolenic acid.
Background technique
Grease is one of Major Nutrient substance of human body, and the grease for taking in human body mainly has following effect: providing for human body Heat;Essential fatty acid (linolenic acid, sub- oil that human body can not be synthesized, must be obtained from food especially vegetable oil are provided Acid);Supplier's body fat soluble vitamin (such as: VA, VD, VE, VK);There is provided flavour of food products and production function (such as: baking and use Oil, sesame oil fragrance etc.).With the raising of human lives' water product, food ration of the people to high heat, high grease, high sugar food Increase, causes more and more crowds with diseases such as obesity, cardiovascular disease, " three high ".How in terms of the diet in prevention State the key issues that disease is studied as medical field, nutrition educational circles.
Alpha-linolenic acid is a kind of essential fatty acid that human body itself can not synthesize, and has ROS, MDA in scavenger-cell, is increased CAT, SOD enzyme activity repair endothelial cell damage, maintain body cell membrane function, adjust blood lipid, reduce cholesterol, anti-inflammatory, resist Cancer, a variety of important physiological functions and pharmacological activity such as enhancing cerebral nerve function.Alpha-linolenic acid can go to satisfy in human liver With generate eicosapentaenoic acid (C20:5) and docosahexaenoic acid (C22:6) under the action of enzyme, chain elongation enzyme, i.e. EPA with DHA, both of which are the important composition ingredients of neuron membrane.EPA has reduction blood lipid and improves platelet aggregation effect, can Prevent the diseases such as cerebral thrombosis and myocardial infarction.DHA, which has, to be promoted nervous system development, improves memory and retinal reflex energy Power, prevention senile dementia, pre- anti-cancer and agaist allergic symptoms and other effects.
The fatty acid that linoleic acid cannot be synthesized as human body itself, role is very multiple in terms of metabolism and health It is miscellaneous.It can be used as energy donor, after mitochondrial oxidation, provide metabolism institute required ATP for cell.Linoleic addition is one Determine to reduce the fatty acid oxidation energy supply in cell in the expression of mRNA level in-site inhibition cell PPAR δ in degree, can be used as Lipid-metabolism key gene PPAR δ inhibitor.
Studies have shown that including " essential fatty acid " (linoleic acid, linolenic acid), in fatty acid total amount needed for human body one day Having 50% from edible oil, often eats certain single oil, the intake that will lead to certain or several fatty acid is not enough or excessive, To make the metabolic balance of human body change, so as to cause various diseases.It is oily both at home and abroad since the 1990s Rouge dietician has carried out a large amount of research, western developed country, the United Nations's grain in the physiological metabolism of human body to various fatty acid The ratio that agriculture tissue and the World Health Organization take in various fatty acid to human body proposes corresponding standard.Chinese nutrition in 2000 Association also proposed recommendation ratio: 1. saturated fatty acid (SFA), monounsaturated fatty acids (MUFA), polyunsaturated fatty acid (PUFA) mass values are 1:1:1.2. the mass values of ω -6 and ω -3 are (4-6) in polyunsaturated fatty acid: 1.There is text It offers and reports that Excess free enthalpy linoleic acid will inhibit conversion of the linolenic acid to EPA and DHA, there is scientist to propose more insatiable hungers thus It should be (1-3) with the mass values of ω -6 in fatty acid and ω -3: 1.
Saturated fatty acid, the monounsaturated fatty acids, how unsaturated ingested based on the above issues with internal authority to human body Fatty acid, the proportion requirement of ω -6 and ω -3;In view of in daily life, people have ingested the saturated fat of sufficient amount Acid, the less status of the serial linolenic acid amount of the ω -3 that ingests;We select the higher plant blend oil of content of polyunsaturated fatty acid As the substitute of deep sea fish oil, laying stress on and mix a kind of ω -6, ω -3 ratio is suitable for, alpha-linolenic acid content is higher, And meet certain saturated fatty acid, monounsaturated fatty acids, polyunsaturated fatty acid ratio ready-mixed oil to meet body to must Need the demand of fatty acid, EPA, DHA.Various fatty acid composition ratio are coordinated simultaneously, meet diet nutritional guide recommended amounts.Raw material Oil is that cold press is obtained through physical refining, does not add any chemical synthesis antioxidant, the activity rich in a large amount of protection cardiovascular and cerebrovasculars Ingredient has significant defencive function to the cardiovascular and cerebrovascular of human body.
Summary of the invention
It is an object of the invention to the illness rate remained high for diseases such as existing crowd's angiocarpy and market ready-mixed oils The functional statuses such as not strong, provide a kind of cardiovascular ready-mixed oil of protection.Ready-mixed oil ω -6, ω -3 ratio are suitable for α-flax Acid content is higher, and meets certain saturated fatty acid, monounsaturated fatty acids, polyunsaturated fatty acid ratio;It does not add and appoints What chemical synthesis antioxidant is easily absorbed by the human body and converts generation EPA, DHA, has and protect cardiovascular function;It can be big Technical scale production.
To achieve the goals above, cardiovascular ready-mixed oil is protected the present invention provides a kind of,
The ready-mixed oil is made of following raw material: each vegetable oil in parts by weight, 10-45 parts of linseed oil, purple perilla seed 25-55 parts oily, 0-5 parts of peony seed oil, 5-20 parts of walnut oil, 0-10 parts of eucommia ulmoides seed oil, 0-15 parts of tomato seed oil, safflower seed oil 0- 35 parts, 0-15 parts of acer truncatum seed oil, 1-8 parts of black sesame oil.
Preferred feature is as follows:
Select linseed oil, eucommia ulmoides seed oil, peony seed oil, Purple Perilla Seed Oil, walnut oil, tomato seed oil, safflower seed oil, ingot Maple seed oil, at least five kinds of black sesame oil deployed.
It is preferred that linseed oil, eucommia ulmoides seed oil, peony seed oil, Purple Perilla Seed Oil, safflower seed oil, at least five kinds of black sesame oil into Row allotment.
More preferably there is linseed oil, Purple Perilla Seed Oil rich in alpha-linolenic acid, tomato seed oil, Du rich in natural anti-oxidation Secondary seed oil, walnut oil, black sesame oil are deployed, for guaranteeing the shelf-life of product.
The ready-mixed oil according to acquired in patent, in addition to that (can turn in vivo rich in omega-3 polyunsaturated fatty acids alpha-linolenic acid It is melted into EPA, DHA) outside, there are also the other active components of a large amount of protection blood vessels: lignan, apiolin, baicalein, luteolin, Perillene, niacin, VE, carrotene, lycopene, kryptoxanthin, phosphatide, squalene, rosin element disaccharide glucoside, Aucubin, Liriodendrin, ligustrin, plant flavone and nervonic acid, alkaloid, oryzanol, sterol etc..Through internal experiment in vitro, show have There are ROS, MDA in scavenger-cell;Increase CAT, SOD enzyme activity;Repair endothelial cell damage;Maintain body cell membrane function;It is anti- Thrombus, reducing blood lipid, blood pressure lowering, reduction cholesterol and reduction atherosclerosis etc. significantly protect cardiovascular function.
The various feedstock oils of physical refining are rich in a large amount of anthocyanin and VE after cold press, have significant oxidation resistance, from And the shelf life of ready-mixed oil is ensured, while being also the important activity component of protection human mechanism.The metabolite of alpha-linolenic acid There is mild adjustment effect to blood lipid metabolism, plasma low density lipoprotein LDL can be promoted to the conversion of high-density lipoprotein HDL, Low-density lipoprotein LDL reduction, high-density lipoprotein HDL is promoted to increase, so that reaching reduces blood lipid, atherosclerosis Purpose.The metabolite of alpha-linolenic acid can expand blood vessel, enhancing blood vessel elasticity to play antihypertensive effect and prevention heart and brain Angiosis effect.Alpha-linolenic acid can change platelet membrane fluidity, so that it is small to the reactivity and blood of stimulation to change blood platelet The number of plate surface receptor.Therefore it can effectively prevent the formation of thrombus.
Other effective components in ready-mixed oil: rosin element disaccharide glucoside, Aucubin etc. have hypotensive activity;Liriodendron Alkali, the inhibitor that ligustrin is angiotensins and cAMP, it plays regulatory role angiocarpy and can increase coronary blood Flow;Niacin (niacin, pellagra preventive factor) can make vascular wall keep elasticity, maintain its function, prevent vascular sclerosis and rupture, also Peripheral vessel can be expanded, thrombosis is prevented, reduces the level of triglycerides in blood;Alkaloid, oryzanol, sterol have significant Reducing blood lipid and cardiovascular protective effect.
Beneficial effects of the present invention are as follows:
1. the present invention is that the alpha-linolenic acid of 20%-48%, linoleic acid accounts for gross weight containing each vegetable fatty acids mass percent 25%-45%, saturated fatty acid total amount < 10%, saturated fatty acid: monounsaturated fatty acids: polyunsaturated fatty acid=1:(2- 2.5): (7-9), ω -6 fatty acid: omega-fatty acid=(0.5-6): 1;It is in not in conjunction with diseases such as the existing crowd's angiocarpy of China Under illness rate, the not strong status of Chinese market ready-mixed oil functionality;It establishes in 2013 editions Chinese residents diet nutritional handbooks, in Nutrition Society, state recommends human body to take in various fatty acid proportions, and Chinese residents saturated fatty acid is ingested on the basis of more grade;It can be with Chinese residents are helped to ingest the fatty acid of rational proportion, and the ready-mixed oil is easily absorbed by the human body and is converted into EPA, DHA, there is guarantor The effect of shield heart blood vessel.
2. vegetable oil raw materials of the present invention for ready-mixed oil preparation use cold press, physics degumming tech, leaching can be got rid of The techniques such as oil solvent residual, depickling, deodorization, decoloration can utmostly protect the biological value of ω -6, ω -3 series fatty acid, Avoid the loss etc. of liposoluble vitamin (VA, VD, VE, VK etc.);Without chemical synthesis antioxidant, prevent food from source The generation of safety problem;It not only ensure that shelf life, but also more green safe.
It, can industrial mass life 3. the present invention is by selecting five kinds and five kinds or more vegetable oil to be deployed, easy to operate It produces.
4. ready-mixed oil prepared by the present invention does not add any chemical synthesis antioxidant, rich in a large amount of protection cardiovascular and cerebrovasculars Active constituent, there is significant defencive function to the cardiovascular and cerebrovascular of human body.
Detailed description of the invention
The influence of oxygen scavenging activity in Fig. 1 difference patent ready-mixed oil concentration versus cell.
Fig. 2 various concentration patent ready-mixed oil to HUVEC cellular oxidation stress influence.
Influence of Fig. 3 various concentration patent ready-mixed oil to oxidoreductase activity in HUVEC cell.
Influence (the amplification that PPAR δ in human umbilical vein epithelial cell (HUVEC) is expressed in the reconciliation oil processing of Fig. 4 various concentration 400 times).
Specific embodiment
The present invention is further illustrated with the following Examples, but these embodiments are merely to illustrate the present invention, are not used in limit The scope of the present invention processed.In addition to specified otherwise or mark, various plant material oil are deployed at room temperature by mass percentage Obtain inventive formulation ready-mixed oil.
Term used herein is identical as grease profession or the skilled scientific words of expert in the art.It is described in the text Best allotment ratio and implementation method only play an exemplary role.The material used herein arrived is as follows:
Linseed oil Xiamen Nong Tekang Biotechnology Co., Ltd
Purple Perilla Seed Oil Xiamen Nong Tekang Biotechnology Co., Ltd
Walnut oil Xiamen Nong Tekang Biotechnology Co., Ltd
Black sesame oil Xiamen Nong Tekang Biotechnology Co., Ltd
Tomato seed oil Xiamen Nong Tekang Biotechnology Co., Ltd
Safflower seed oil Xiamen Nong Tekang Biotechnology Co., Ltd
Eucommia ulmoides seed oil Xiamen Nong Tekang Biotechnology Co., Ltd
Peony seed oil Xiamen Nong Tekang Biotechnology Co., Ltd
Acer truncatum seed oil Xiamen Nong Tekang Biotechnology Co., Ltd
Embodiment 1:
Table 1-1: vegetable fatty acids ratio
Table 1-2: vegetable fatty acids ratio
1) is 10 parts of linseed oil in parts by weight, 10 parts of tomato seed oil, 20 parts of safflower seed oil, and 55 parts of Purple Perilla Seed Oil, 5 parts of peony seed oil are deployed;It stirs, is uniformly mixed under room temperature;
2) ready-mixed oil nitrogen charging is sealed in vial by.
3) ready-mixed oil alpha-linolenic acid 41.27%, linoleic acid 28.92%, saturated fatty acid total amount 9.07%, saturated fat obtained by Acid: monounsaturated fatty acids: polyunsaturated fatty acid=1:2:7.7, ω -6 fatty acid: omega-fatty acid=0.7:1.
1. the influence that patent ready-mixed oil removes reactive oxygen species
From fig. 1, it can be seen that patent formulation ready-mixed oil has the function of active oxygen in scavenger-cell, maximum scavenging effect reaches 21.5%。
2. patent ready-mixed oil to HUVEC cellular oxidation stress influence
As can be seen from Figure 2, patent formulation ready-mixed oil have the function of Cellular Oxidation stress, concentration for the treatment of with stress effect be in It is now positively correlated, MDA highest can reduce by 45.8% in cell.
3. influence of the patent ready-mixed oil to cellular redox enzymatic activity
As can be seen from Figure 3, patent formulation ready-mixed oil can be improved the activity of cellular redox enzyme, with concentration for the treatment of Increase, the enzyme activity of hepatocuprein (SOD) and catalase (CAT) is consequently increased, and peroxide Scavenging activity increases By force.
4. the influence that patent formulation ready-mixed oil expresses lipid-metabolism gene PPAR δ
As can be seen from Figure 4, patent formulation, which reconciles oil processing, can influence cytolipin metabolism, with the increase of concentration for the treatment of, The expression of peroxisome Proliferator-activated receptor (PPAR δ) increases, and inhibits oxidative metabolism of the fatty acid in cell, thus The energy charge for reducing cell is horizontal, inducing cell damage.
Embodiment 2:
Table 2-1: vegetable fatty acids ratio
Table 2-2: vegetable fatty acids ratio
1) is 10 parts of linseed oil in parts by weight, 10 parts of eucommia ulmoides seed oil, 20 parts of walnut oil, and 5 parts of black sesame oil, purple perilla 45 parts of seed oil, 10 parts of oil of acer truncatum seed are deployed;It stirs, is uniformly mixed under room temperature;
2) ready-mixed oil nitrogen charging is sealed in vial by.
3) ready-mixed oil alpha-linolenic acid 41.31%, linoleic acid 26.59%, saturated fatty acid total amount 8.17%, saturated fat obtained by Acid: monounsaturated fatty acids: polyunsaturated fatty acid=1:2.4:8.3, ω -6 fatty acid: omega-fatty acid=0.6:1.
4) it is reachable to reduce intracellular MDA up to 22.4% for active oxygen effect in the maximum scavenger-cell of patent formulation ready-mixed oil 46.0%。
Embodiment 3:
Table 3-1: vegetable fatty acids ratio
1) is 20 parts of linseed oil in parts by weight, 5 parts of tomato seed oil, 35 parts of safflower seed oil, and 35 parts of Purple Perilla Seed Oil, core 5 parts of peach oil is deployed;It stirs, is uniformly mixed under room temperature;
2) ready-mixed oil nitrogen charging is sealed in vial by.
3) ready-mixed oil alpha-linolenic acid 34.24%, linoleic acid 37.78%, saturated fatty acid total amount 8.37%, saturated fat obtained by Acid: monounsaturated fatty acids: polyunsaturated fatty acid=1:2.1:8.5, ω -6 fatty acid: omega-fatty acid=1.1:1.
4) it is reachable to reduce intracellular MDA up to 22.7% for active oxygen effect in the maximum scavenger-cell of patent formulation ready-mixed oil 46.5%。
Embodiment 4:
Table 4-1: vegetable fatty acids ratio
1) is 15 parts of tomato seed oil in parts by weight, 5 parts of sesame oil, 10 parts of oil of acer truncatum seed, and 35 parts of safflower seed oil, Du It secondary 35 parts of seed oil, is deployed;It stirs, is uniformly mixed under room temperature;
2) ready-mixed oil nitrogen charging is sealed in vial by.
3) ready-mixed oil alpha-linolenic acid 23.03%, linoleic acid 42.99%, saturated fatty acid total amount 9.26%, saturated fat obtained by Acid: monounsaturated fatty acids: polyunsaturated fatty acid=1:2.1:7.1, ω -6 fatty acid: omega-fatty acid=1.9:1.
4) it is reachable to reduce intracellular MDA up to 20.4% for active oxygen effect in the maximum scavenger-cell of patent formulation ready-mixed oil 45.3%。
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with Modification, is all covered by the present invention.

Claims (4)

1. one kind protects cardiovascular ready-mixed oil rich in alpha-linolenic acid, it is characterised in that: the ready-mixed oil is by following raw material group At: each vegetable oil is in parts by weight, 10 parts of linseed oil, 10 parts of tomato seed oil, 20 parts of safflower seed oil, 55 parts of Purple Perilla Seed Oil, male Red 5 parts of seed oil;Alpha-linolenic acid 41.27% in the ready-mixed oil, linoleic acid 28.92%, saturated fatty acid total amount 9.07%, saturation Fatty acid: monounsaturated fatty acids: polyunsaturated fatty acid=1:2:7.7, ω -6 fatty acid: omega-fatty acid=0.7:1.
2. one kind protects cardiovascular ready-mixed oil rich in alpha-linolenic acid, it is characterised in that: the ready-mixed oil is by following raw material group In parts by weight at: each vegetable oil, 10 parts of linseed oil, 10 parts of eucommia ulmoides seed oil, 20 parts of walnut oil, 5 parts of black sesame oil, purple perilla seed 45 parts of oil, 10 parts of oil of acer truncatum seed;Alpha-linolenic acid 41.31% in the ready-mixed oil, linoleic acid 26.59%, saturated fatty acid is total Amount 8.17%, saturated fatty acid: monounsaturated fatty acids: polyunsaturated fatty acid=1:2.4:8.3, ω -6 fatty acid: ω -3 rouge Fat acid=0.6:1.
3. one kind protects cardiovascular ready-mixed oil rich in alpha-linolenic acid, it is characterised in that: the ready-mixed oil is by following raw material group In parts by weight at: each vegetable oil, 20 parts of linseed oil, 5 parts of tomato seed oil, 35 parts of safflower seed oil, 35 parts of Purple Perilla Seed Oil, walnut 5 parts of oil;Alpha-linolenic acid 34.24% in the ready-mixed oil, linoleic acid 37.78%, saturated fatty acid total amount 8.37%, saturated fat Acid: monounsaturated fatty acids: polyunsaturated fatty acid=1:2.1:8.5, ω -6 fatty acid: omega-fatty acid=1.1:1.
4. one kind protects cardiovascular ready-mixed oil rich in alpha-linolenic acid, it is characterised in that: the ready-mixed oil is by following raw material group At: in parts by weight, 15 parts of tomato seed oil, 5 parts of sesame oil, acer truncatum seed is 10 parts oily for each vegetable oil, and 35 parts of safflower seed oil, purple perilla 35 parts of seed oil;Alpha-linolenic acid 23.03% in the ready-mixed oil, linoleic acid 42.99%, saturated fatty acid total amount 9.26%, saturated fat Fat acid: monounsaturated fatty acids: polyunsaturated fatty acid=1:2.1:7.1, ω -6 fatty acid: omega-fatty acid=1.9:1.
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CN106720461A (en) * 2016-11-17 2017-05-31 中国农业大学 A kind of linseed ready-mixed oil and its concocting method
CN106720470A (en) * 2016-12-29 2017-05-31 天津市百奥生物技术有限公司 A kind of composition with auxiliary antioxidation and preparation method thereof
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