CN105902574A - Single-dosage package arenobufagin and preparation method thereof - Google Patents
Single-dosage package arenobufagin and preparation method thereof Download PDFInfo
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- CN105902574A CN105902574A CN201610397030.XA CN201610397030A CN105902574A CN 105902574 A CN105902574 A CN 105902574A CN 201610397030 A CN201610397030 A CN 201610397030A CN 105902574 A CN105902574 A CN 105902574A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/65—Amphibians, e.g. toads, frogs, salamanders or newts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
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Abstract
The invention discloses single-dosage package arenobufagin. The single-dosage package arenobufagin is characterized in that white slurry of ear-side glands and dermal gland of bufo bufo gargarigans or bufo melanostictus is taken to be screened to remove impurities and to be dried at low temperature, and smashing is conducted to obtain arenobufagin powder. Single-dosage package is conducted according to the adult single dosage of the total amount, being 0.08-10 mg, of cinobufagin and resibufogenin, and the package difference limit is smaller than or equal to +/-15% according to the net weight, being 1-100 mg, of arenobufagin in the single-dosage package. The small-package arenobufagin is suitable for traditional Chinese medicine decoctions, pills and powder and suitable for external application. By means of the low-temperature drying, particularly freezing drying technology, active components of arenobufagin are kept to the maximum extent; by strictly controlling the total amount of cinobufagin and resibufogenin in the single-dosage package, the use safety problem of arenobufagin is effectively solved, and the last barrier is broken for clinical application of arenobufagin.
Description
Technical field
The invention belongs to the field of Chinese medicines, be specifically related to a kind of technique advanced person, steady quality, dosage accurate,
Use safe unit dose package Venenum Bufonis and preparation method thereof.
Background technology
Venenum Bufonis (Bufonis Venenum) is Bufonidae animal socket of the eye Bufo siccus (Bufo melanositictus
Schneider) or Bufo siccus (Bufo bufo gargarizans Cantor) dry secretions (China
First version in 2015 of people's republic's pharmacopeia).Venenum Bufonis begins to be loaded in the most lost book on Chinese herbal medicine " property of medicine Tang Dynasty
Opinion ", original name Bufo siccus eyebrow fat.There is removing toxic substances, pain relieving, the effect of refreshment of having one's ideas straightened out, treat skin ulcer for carbuncle,
Laryngopharynx swelling and pain, heatstroke coma, erputive abdominal disease is vomited and diarrhoea.Enter ball more and dissipate use.Suitable amount used externally.
Containing substantial amounts of bufadienolide (bufadienolide) compounds in Venenum Bufonis, such as bufotalien
(Bufotalin), Toadpoison Medicine (bufalin), cinobufagin (cinobufagin), bufogenin
(resibufogenin) etc., they are the principle active component of Bufo siccus serosity, Venenum Bufonis, be Venenum Bufonis heart tonifying,
Sedation and analgesia, antiinflammatory and antitumor and the material base of radiation resistance, 2015 editions Chinese Pharmacopoeias one
Portion defines the total amount of Venenum Bufonis cinobufagin and bufogenin must not be less than 6.0%.
Li Shizhen (1518-1593 A.D.) has recorded traditional harvesting and processing method in Compendium of Material Medica: " take Venenum Bufonis to differ,
Or pinch eyebrow rib with hands, take white sauce on oilpaper, and on Folium Mori, insert shady spot's one night i.e. from dry, or peace
Putting the interior Sheng of thick bamboo tube, true person is frivolous, enters taste sweet also.Or receive in mouth, then with the peppery thing such as Bulbus Allii and Fructus Piperis
Toad body white sauce goes out, and combing with bamboo scrapes, and face and in bulk do it ".Modern times then many by the crispest for Venenum Bufonis processing,
Group is crisp or chess piece is crisp, and without unified quantitative criterion, concrete grammar is to be sieved by the Venenum Bufonis serosity adopted
Roguing, is spread evenly across the serosity after roguing on plate glass and is dried in flakes crisp, or pour into and get ready
Model-container in dry agglomerating shortcake or chess piece crisp.Time owing to drying is long, gentle by regional difference
The impact of time condition is big, and the product quality of Venenum Bufonis is difficult to ensure that, from the appearance character such as color and luster, abnormal smells from the patient to
Inherent quality with effective ingredient as representative all has greatest differences, has had a strong impact on product quality and clinic
Curative effect.Chinese Pharmacopoeia has recorded Venenum Bufonis powder, and its concocting method is: take Venenum Bufonis, pulverizes, and adds Chinese liquor leaching
Stain, often stirs to paste, dry, pulverize.Every 10kg Venenum Bufonis Chinese liquor 20kg.
And, Venenum Bufonis is toxic traditional Chinese medicine, must be noted that drug safety clinically.Document is reported, little
Mus oral administration, the LD of Venenum Bufonis50For 0.6006g/kg (Li Xingping, Lei Ling, recklessly the most first-class. Venenum Bufonis
Acute toxicity and DANLING XINSHU JIAONANG studies on acute toxicity. " Pharmacology and Clinics of Chinese Materia Medica " 2012,6:
127-129).For toxic traditional Chinese medicine, safety is it is critical that factor, and taking dose is to determine
The key of Venenum Bufonis safety.Version Chinese Pharmacopoeia in 2015 defines the taking dose of Venenum Bufonis and is
0.015～0.030g, calculate according to adult's 60kg body weight, the taking dose of people is 0.00025～0.0005
G/kg, for mice LD501/2400～1/1200, therefore clinically use Venenum Bufonis have safety assurance.
But often lack accurate weighing apparatus due to clinical practice and carry out on-the-spot weighing, so the purposes of Venenum Bufonis is the biggest
Be confined to Chinese medicine compound preparation in degree, as have been approved by listing have Heart pill of Musk, LIUSHEN WAN,
The Chinese patent medicines such as compound recipe toad venom plaster.And its using method uses till today and do not breaks through, the most always
Can obtain actual application in Chinese medicine decoction, key factor therein the most precisely carries because of dosage control exactly
The safety issue come.The Dose Problem of Venenum Bufonis has become as last one of restriction Venenum Bufonis clinical practice
Barrier, and being precisely controlled dosage is the premise effectively solving Venenum Bufonis safety in utilization problem.
Summary of the invention
It is an object of the invention to provide a kind of technique advanced person, steady quality, dosage is accurate, use peace
Full unit dose package Venenum Bufonis and preparation method thereof.
Technical scheme is as follows:
A kind of unit dose package Venenum Bufonis, it is characterised in that according to Venenum Bufonis cinobufagin and fat toadpoison
Adult's single taking dose of aglucon total amount 0.08～10mg carries out unit dose package, content uniformity limit
Less than or equal to ± 15% in terms of Venenum Bufonis nt wt net weight in unit dose package 1～100mg.
Preferably, total according to Venenum Bufonis cinobufagin and bufogenin of this unit dose package Venenum Bufonis
Amount is 0.5～5.0mg to carry out subpackage dress, and content uniformity limit is with Venenum Bufonis nt wt net weight in unit dose package
10～50mg meters are less than or equal to ± 10%.
The preparation method of above-mentioned unit dose package Venenum Bufonis comprises the steps:
1) take Chinese crude drug Venenum Bufonis, test by national standard, detection effective ingredient cinobufagin and fat
Bufotalin content；
2) take the Venenum Bufonis that inspection is qualified, pulverize to obtain Venenum Bufonis powder, add less than or equal to Venenum Bufonis grain weight amount 10
Times water, Chinese liquor or ethanol water, stir, obtain toad venom plaster:
3) taking of toad venom plaster is calculated according to cinobufagin and bufogenin content and extension rate
Dosage, precision measures the toad venom plaster of respective volume, is sub-packed in unit dose package container, cold drying,
Obtain unit dose package Venenum Bufonis；Or, take toad venom plaster cold drying, pulverize, obtain Venenum Bufonis powder, detect toad
Crisp powder cinobufagin and the total amount of bufogenin, calculate the taking dose of Venenum Bufonis powder, be sub-packed in
In unit dose package container, obtain unit dose package Venenum Bufonis.
Or:
1) take Bufo siccus or black surround Bufo siccus ear rear gland, the white serosity of glandular integumentaria, obtain fresh Venenum Bufonis,
Cross 100～200 mesh sieves and remove impurity, obtain toad venom plaster；Or take fresh Venenum Bufonis, add less than or equal to Venenum Bufonis weight
Measure the water of 10 times amount, Chinese liquor or ethanol water, stir, cross 100～200 mesh sieves and remove impurity,
Obtain toad venom plaster；
2) detection toad venom plaster cinobufagin and the total amount of bufogenin, calculates taking of toad venom plaster
Dosage, precision measures the toad venom plaster of respective volume, is sub-packed in unit dose package container, cold drying,
Obtain unit dose package Venenum Bufonis；Or, take toad venom plaster cold drying, pulverize, obtain Venenum Bufonis powder, detect toad
Crisp powder cinobufagin and the total amount of bufogenin, calculate the taking dose of Venenum Bufonis powder, be sub-packed in
In unit dose package container, obtain unit dose package Venenum Bufonis.
In described ethanol water, the volumetric concentration of ethanol is less than or equal to 95%.
The cold drying of described toad venom plaster is lyophilization, be vacuum dried and dry in one or more.
Described cryodesiccated critical craft is cooling rate 0.2～2.0 DEG C/min, precooling temperature
-15～-50 DEG C, drying chamber pressure 10～150 handkerchief, temperature of heating plate 30～50 DEG C, material thickness 0.1～3
Centimetre, drying time 6～72 hours.
Described vacuum drying critical craft is temperature 40～-80 DEG C, vacuum 0.06～0.1 MPa,
Material thickness 0.1～3 centimetres, drying time 6～48 hours.
The fineness of described Venenum Bufonis powder is in coarse powder, middle powder, fine powder, fine powder, impalpable powder, micropowder
Any one.
Described unit dose package container uses pharmaceutically conventional packaging material, including vial, plastics
One in bottle, canister, plastic bag, paper bag, aluminum aluminum-plastic packaged, double packaging.
The unit dose package Venenum Bufonis of the present invention can be made into for oral decoction, pill, powder or external,
It is administered orally after being directly administered orally as employing during decoction or decoct altogether with other prepared slices of Chinese crude drugs, employing when being directly administered orally
Any one of water, milk, Chinese medicine decoction, beverage or fruit juice take or take.Can also first by
Venenum Bufonis pours into capsule, or with edible paper, food adhere to/parcel after put in mouth, then with water, milk,
Chinese medicine decoction, beverage or fruit juice take, and adhere to or wrap up the food of Venenum Bufonis include rice, noodles, won ton
Any one of Tun, cake, veterinary antibiotics, cake, confection.
Owing to present invention employs the advanced drying process such as lyophilization, vacuum drying, especially freezing
Being dried, baking temperature is low, and the destruction for active component is minimum, remains Venenum Bufonis to greatest extent
Active component, freeze-drying method the Venenum Bufonis cinobufagin prepared and the total amount of bufogenin
Reach more than 8.5%, higher than tradition dries method content.Prevent fresh Venenum Bufonis the most simultaneously
The product so caused because of reasons such as oxidation, microbial growth in dry run goes bad, it is ensured that product
Freshness.And, strictly control cinobufagin and fat toad by precision weighing and unit dose package
The total amount of poison aglucon, effectively solves the safety in utilization problem of Venenum Bufonis, and the clinical practice for Venenum Bufonis is abolished
Last one barrier.
Detailed description of the invention
Present pre-ferred embodiments is given below, to describe technical scheme in detail.
Embodiment 1
Take Venenum Bufonis 100g, pulverized 100 mesh, obtain Venenum Bufonis fine powder.According to People's Republic of China's medicine
Standard test under one Venenum Bufonis item of allusion quotation, detection Venenum Bufonis powder effective ingredient cinobufagin and bufogenin
Content is 6.67%.It is the analytical balance of 0.01mg by precision, joins according to cinobufagin and fat toadpoison
The total amount of base is that 1.00mg precision weighs Venenum Bufonis powder 15.00mg, is sub-packed in vial, and spiral cover is close
Envelope, checks that content uniformity limit is-4.27%～+5.80%, obtains unit dose package Venenum Bufonis.
Embodiment 2
Take Venenum Bufonis 100g, pulverized 80 mesh, obtain Venenum Bufonis fine powder.According to Pharmacopoeia of People's Republic of China one
Standard test under portion's Venenum Bufonis item, the total amount of detection effective ingredient cinobufagin and bufogenin is
7.62%.It is the analytical balance of 0.01mg by precision, total according to cinobufagin and bufogenin
Amount weighs Venenum Bufonis powder 50.00mg for 3.81mg, is sub-packed in little plastic bottle, and spiral cover seals, and checks dress
Amount difference limit is-2.36%～+4.34%, obtains unit dose package Venenum Bufonis.
Embodiment 3
Take Venenum Bufonis 100g, pulverized 65 mesh, obtain powder in Venenum Bufonis, then pulverize to obtain Venenum Bufonis with jet mill
Micropowder.According to standard test under one Venenum Bufonis item of Pharmacopoeia of People's Republic of China, detection Venenum Bufonis powder has
Effect composition cinobufagin and bufogenin content are 7.30%, with the analysis point sky that precision is 0.01mg
Flat, it is that 0.73mg precision weighs Venenum Bufonis powder according to the total amount of cinobufagin and bufogenin
10.00mg, is sub-packed in two-layer compound aluminium foil, seal, check content uniformity limit be-2.80%～+
6.70%, obtain unit dose package Venenum Bufonis.
Embodiment 4
Take the white serosity 200g of Bufo siccus ear rear gland, cross 120 mesh sieves and remove impurity.Put freezing
In 0.2 DEG C/min of cooling rate in drying machine, precooling temperature-40 DEG C, drying chamber pressure 10 handkerchief, material
Thickness 1 centimetre, under the conditions of temperature of heating plate 35 DEG C, lyophilizing 12 hours, pulverized 80 mesh, obtained Venenum Bufonis
Fine powder.Detection active component cinobufagin and bufogenin content are 9.13%, according to China's Venenum Bufonis poison
The total amount of base and bufogenin be 2.74mg calculate Venenum Bufonis powder single taking dose be 30.00mg,
Use High Precision Automatic powder racking machine to be sub-packed in plastic bottle, seal, check that content uniformity limit is
-2.93%～+5.57%, obtain unit dose package Venenum Bufonis.
Embodiment 5
Take the white serosity 200g of Bufo siccus ear rear gland, add 2 times of volume 95% ethanol waters,
Stir, cross 150 mesh sieves and remove impurity.Put decompression in freezer dryer and remove major part ethanol,
In 0.7 DEG C/min of cooling rate, precooling temperature-20 DEG C, drying chamber pressure 20 handkerchief, material thickness 0.1
Centimetre, under the conditions of temperature of heating plate 30 DEG C, lyophilizing 6 hours, pulverized 120 mesh, obtained Venenum Bufonis fine powder.
Detection active component cinobufagin and bufogenin content are 10.00%, according to cinobufagin and
The total amount of bufogenin is the single taking dose 100.00mg that 10.00mg calculates Venenum Bufonis powder, uses
High Precision Automatic powder racking machine is sub-packed in vial, seals, and checks that content uniformity limit is
-4.70%～+6.68%, obtain unit dose package Venenum Bufonis.
Embodiment 6
Take the white serosity 200g of black surround Bufo siccus ear rear gland, add 10 times of volume Chinese liquor, stir,
Cross 100 mesh sieves and remove impurity.Put decompression in freezer dryer and remove major part ethanol, in cooling rate
1.0 DEG C/min, precooling temperature-50 DEG C, drying chamber pressure 60 handkerchief, material thickness 3 centimetres, heating plate
Under the conditions of temperature 40 DEG C, lyophilizing 24 hours, pulverized 100 mesh, obtained Venenum Bufonis fine powder.Detection activity becomes
The content dividing cinobufagin and bufogenin is 8.74%, according to cinobufagin and bufogenin
Total amount be 4.37mg calculate Venenum Bufonis powder single taking dose be 50.00mg, precision weigh single clothes
With the Venenum Bufonis powder of dosage, it is sub-packed in two-layer compound aluminium foil, seals, check that content uniformity limit is
-3.26%～+3.82%, obtain unit dose package Venenum Bufonis.
Embodiment 7
Take the white serosity 200g of Bufo siccus ear rear gland, add 5 times of volume 50% ethanol waters,
Stir, cross 200 mesh sieves and remove impurity.Detection active component cinobufagin and bufogenin
Content be 0.25%, according to the total amount of cinobufagin and bufogenin be 3.0mg calculate Venenum Bufonis liquid
Single taking dose be 1.2ml, precision weighs the Venenum Bufonis liquid of single taking dose, uses automatic subpackaging
Machine is sub-packed in aluminium pot, puts decompression in freezer dryer and removes major part ethanol, in cooling rate 2.0 DEG C
/ min, precooling temperature-35 DEG C, drying chamber pressure 150 handkerchief, lyophilizing 72 under the conditions of temperature of heating plate 50 DEG C
Hour, seal, check that content uniformity limit is-3.81%～+6.74%, obtain unit dose package Venenum Bufonis.
Embodiment 8
Taking the white serosity 200g of black surround Bufo siccus ear rear gland, add 4 times of volume 2 drinking waters, stirring is all
Even, cross 150 mesh sieves and remove impurity.Put in vacuum drier in material thickness 1 centimetre, temperature 60 C,
It is vacuum dried under the conditions of vacuum 0.09 MPa, pulverized 100 mesh, and obtained Venenum Bufonis fine powder.Detection is lived
The content of property composition cinobufagin and bufogenin is 8.00%, according to cinobufagin and fat toadpoison
The total amount of aglucon be 0.08mg calculate Venenum Bufonis powder single taking dose be 1.00mg, precision weighs single
The Venenum Bufonis powder of taking dose, is sub-packed in aluminum-plastic packaged interior, seals, and checks that content uniformity limit is
-11.03%～+9.47%, obtain unit dose package Venenum Bufonis.
Embodiment 9
Take the white serosity 200g of Bufo siccus ear rear gland, add 5 times of volume 60% ethanol waters,
Stir, cross 150 mesh sieves and remove impurity.Put in vacuum drier in material thickness 0.1 centimetre, temperature
Spend 80 DEG C, be vacuum dried under the conditions of vacuum 0.06 MPa, pulverized 100 mesh, and obtained Venenum Bufonis fine powder.
Detection active component cinobufagin and the content of bufogenin are 7.20%, according to cinobufagin and
The total amount of bufogenin is that to calculate the single taking dose of Venenum Bufonis powder be 100.00mg to 7.20mg, accurate
Weigh the Venenum Bufonis powder of single taking dose, use High Precision Automatic racking machine to be sub-packed in vial, close
Envelope, checks that content uniformity limit is-5.28%～+4.34%, obtains unit dose package Venenum Bufonis.
Embodiment 10
Take the white serosity 200g of Bufo siccus ear rear gland, add 4 times of volume 50% ethanol waters,
Stir, cross 120 mesh sieves and remove impurity.Put in vacuum drier in material thickness 3 centimetres, temperature
Spend 40 DEG C, be vacuum dried under the conditions of vacuum 0.1 MPa, pulverized 120 mesh, and obtained Venenum Bufonis fine powder.
Detection active component cinobufagin and the content of bufogenin are 7.60%, according to cinobufagin and
The total amount of bufogenin is that to calculate the single taking dose of Venenum Bufonis powder be 40.00mg to 3.04mg, accurate
Weigh the Venenum Bufonis powder of single taking dose, use High Precision Automatic racking machine to be sub-packed in vial, close
Envelope, checks that content uniformity limit is-6.27%～+5.38%, obtains unit dose package Venenum Bufonis.
Embodiment 11 comparative examples
Take the white serosity 200g of Bufo siccus ear rear gland, cross 150 mesh sieves and remove impurity, put on glass plate in
Dry at Yin Liang, pulverized 100 mesh, obtain Venenum Bufonis fine powder.Detection active component cinobufagin and
The content of bufogenin is 6.43%.
The ultimate principle of the present invention, principal character and advantages of the present invention have more than been shown and described.This
Skilled person will appreciate that of industry, the present invention is not restricted to the described embodiments, above-described embodiment and
The principle that the present invention is simply described described in description, before without departing from spirit and scope of the invention
Putting the present invention and also have various changes and modifications, these changes and improvements both fall within claimed basis
In invention scope.
Claims (9)
1. a unit dose package Venenum Bufonis, it is characterised in that according to cinobufagin and bufogenin
Adult's single taking dose of total amount 0.08～10mg carries out unit dose package, and content uniformity limit is with list
In dose package, Venenum Bufonis nt wt net weight 1～100mg meter is less than or equal to ± 15%.
Unit dose package Venenum Bufonis the most according to claim 1, it is characterised in that according in Venenum Bufonis
The total amount of cinobufagin and bufogenin is 0.5～5mg to carry out unit dose package, and content uniformity limits
Degree is less than or equal to ± 10% in terms of Venenum Bufonis nt wt net weight in unit dose package 10～50mg.
3. the preparation method of the unit dose package Venenum Bufonis described in claim 1 or 2, it is characterised in that bag
Include following steps:
1) take Chinese crude drug Venenum Bufonis, contain by national standard detection effective ingredient cinobufagin and bufogenin
Amount；
2) take the Venenum Bufonis that inspection is qualified, pulverize to obtain Venenum Bufonis powder, add less than or equal to Venenum Bufonis grain weight amount 10
Water, Chinese liquor or ethanol water again, stirs, obtains toad venom plaster；
3) taking of toad venom plaster is calculated according to cinobufagin and bufogenin content and extension rate
Dosage, precision measures the toad venom plaster of respective volume, is sub-packed in unit dose package container, cold drying,
Obtain unit dose package Venenum Bufonis；Or, take toad venom plaster, cold drying, pulverize, obtain Venenum Bufonis powder, detection
Venenum Bufonis powder cinobufagin and the total amount of bufogenin, calculate the taking dose of Venenum Bufonis powder, subpackage
In unit dose package container, obtain unit dose package Venenum Bufonis.
4. the preparation method of the unit dose package Venenum Bufonis described in claim 1 or 2, it is characterised in that bag
Include following steps:
1) take Bufo siccus or black surround Bufo siccus ear rear gland, the white serosity of glandular integumentaria, obtain fresh Venenum Bufonis.
Take fresh Venenum Bufonis and cross 100～200 mesh sieves removing impurity, obtain toad venom plaster；Or take fresh Venenum Bufonis, add and be less than
In water, Chinese liquor or the ethanol water of Venenum Bufonis weight 10 times amount, cross 100～200 mesh sieves and remove impurity,
Toad venom plaster；
2) detection toad venom plaster cinobufagin and the total amount of bufogenin, calculates taking of toad venom plaster
Dosage, precision measures the toad venom plaster of respective volume, is sub-packed in unit dose package container, cold drying,
Obtain unit dose package Venenum Bufonis；Or, take toad venom plaster cold drying, pulverize, obtain Venenum Bufonis powder, detect toad
Crisp powder cinobufagin and the total amount of bufogenin, calculate the taking dose of Venenum Bufonis powder, be sub-packed in
In unit dose package container, obtain unit dose package Venenum Bufonis.
5. the preparation method of the unit dose package Venenum Bufonis described in claim 3 or 4, it is characterised in that second
In alcohol-water solution, the volumetric concentration of ethanol is less than or equal to 95%.
6. the preparation method of the unit dose package Venenum Bufonis described in claim 3 or 4, it is characterised in that
The cold drying of described toad venom plaster is lyophilization, be vacuum dried and dry in one or more.
7. the preparation method of the unit dose package Venenum Bufonis described in claim 6, it is characterised in that freezing
The critical craft being dried is cooling rate 0.2～2.0 DEG C/min, precooling temperature-15～-50 DEG C, is dried
Chamber pressure 10～150 handkerchief, temperature of heating plate 30～50 DEG C, material thickness 0.1～3 centimetres, drying time
6～72 hours.
8. the preparation method of the unit dose package Venenum Bufonis described in claim 6, it is characterised in that vacuum
The critical craft being dried is temperature 40～-80 DEG C, vacuum 0.06～0.1 MPa, material thickness 0.1～3
Centimetre, drying time 6～48 hours.
9. the preparation method of the unit dose package Venenum Bufonis described in claim 3 or 4, it is characterised in that institute
Stating the fineness of Venenum Bufonis powder is any in coarse powder, middle powder, fine powder, fine powder, impalpable powder, micropowder
A kind of.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107158039A (en) * | 2017-06-28 | 2017-09-15 | 四川仟源中药饮片有限公司 | A kind of direct oral medicinal slice of the high-purity dried venom of toads and preparation method thereof |
| CN107456773A (en) * | 2017-09-26 | 2017-12-12 | 杨鸣泽 | Dried venom of toads fast decompression drying shed and its drying means |
| CN109481471A (en) * | 2019-01-25 | 2019-03-19 | 上海真仁堂药业有限公司 | The preparation method of dried venom of toads powder medicine materical crude slice |
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2016
- 2016-06-03 CN CN201610397030.XA patent/CN105902574B/en active Active
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| 国家药典委员会: "《中华人民共和国药典 2015年版 一部》", 31 July 2015 * |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107158039A (en) * | 2017-06-28 | 2017-09-15 | 四川仟源中药饮片有限公司 | A kind of direct oral medicinal slice of the high-purity dried venom of toads and preparation method thereof |
| CN107456773A (en) * | 2017-09-26 | 2017-12-12 | 杨鸣泽 | Dried venom of toads fast decompression drying shed and its drying means |
| CN107456773B (en) * | 2017-09-26 | 2023-03-10 | 海门区杨一堂蟾蜍养殖场 | Toad venom rapid decompression drying room and drying method thereof |
| CN109481471A (en) * | 2019-01-25 | 2019-03-19 | 上海真仁堂药业有限公司 | The preparation method of dried venom of toads powder medicine materical crude slice |
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