CN105861369A - Microbial agent for garbage composting as well as preparation method and application thereof - Google Patents
Microbial agent for garbage composting as well as preparation method and application thereof Download PDFInfo
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- CN105861369A CN105861369A CN201610261042.XA CN201610261042A CN105861369A CN 105861369 A CN105861369 A CN 105861369A CN 201610261042 A CN201610261042 A CN 201610261042A CN 105861369 A CN105861369 A CN 105861369A
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- Prior art keywords
- microbial
- rubbish
- garbage
- bacillus amyloliquefaciens
- candida utilis
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- 239000010813 municipal solid waste Substances 0.000 title claims abstract description 94
- 230000000813 microbial effect Effects 0.000 title claims abstract description 51
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- 238000009264 composting Methods 0.000 title abstract description 5
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims abstract description 44
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 42
- 241000235646 Cyberlindnera jadinii Species 0.000 claims abstract description 39
- 240000001046 Lactobacillus acidophilus Species 0.000 claims abstract description 36
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims abstract description 36
- 230000001580 bacterial effect Effects 0.000 claims abstract description 36
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 36
- 238000000034 method Methods 0.000 claims abstract description 34
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims abstract description 32
- 239000007788 liquid Substances 0.000 claims abstract description 28
- 239000007787 solid Substances 0.000 claims abstract description 23
- 239000002068 microbial inoculum Substances 0.000 claims description 54
- 241000894006 Bacteria Species 0.000 claims description 44
- 239000000243 solution Substances 0.000 claims description 27
- 239000001963 growth medium Substances 0.000 claims description 24
- 239000001888 Peptone Substances 0.000 claims description 16
- 108010080698 Peptones Proteins 0.000 claims description 16
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 16
- 230000004913 activation Effects 0.000 claims description 16
- 235000019319 peptone Nutrition 0.000 claims description 16
- 238000004056 waste incineration Methods 0.000 claims description 15
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 12
- 239000008103 glucose Substances 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 10
- 239000007921 spray Substances 0.000 claims description 9
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 8
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 claims description 8
- 229940041514 candida albicans extract Drugs 0.000 claims description 8
- 239000006071 cream Substances 0.000 claims description 8
- 229910000396 dipotassium phosphate Inorganic materials 0.000 claims description 8
- 239000000843 powder Substances 0.000 claims description 8
- 239000001632 sodium acetate Substances 0.000 claims description 8
- 235000017281 sodium acetate Nutrition 0.000 claims description 8
- 239000011780 sodium chloride Substances 0.000 claims description 8
- 239000000758 substrate Substances 0.000 claims description 8
- 239000012138 yeast extract Substances 0.000 claims description 8
- 229910052564 epsomite Inorganic materials 0.000 claims description 7
- 238000011534 incubation Methods 0.000 claims description 7
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims description 6
- 235000015097 nutrients Nutrition 0.000 claims description 6
- 238000010790 dilution Methods 0.000 claims description 5
- 239000012895 dilution Substances 0.000 claims description 5
- 230000000855 fungicidal effect Effects 0.000 claims description 5
- 239000000417 fungicide Substances 0.000 claims description 5
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 claims description 4
- VTYYLEPIZMXCLO-UHFFFAOYSA-L calcium carbonate Substances [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 4
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 4
- 239000004202 carbamide Substances 0.000 claims description 4
- 235000013877 carbamide Nutrition 0.000 claims description 4
- FPAFDBFIGPHWGO-UHFFFAOYSA-N dioxosilane;oxomagnesium;hydrate Chemical compound O.[Mg]=O.[Mg]=O.[Mg]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O.O=[Si]=O FPAFDBFIGPHWGO-UHFFFAOYSA-N 0.000 claims description 4
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 4
- 238000005286 illumination Methods 0.000 claims description 4
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 4
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 claims description 4
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 claims description 4
- 229920000053 polysorbate 80 Polymers 0.000 claims description 4
- 229910000029 sodium carbonate Inorganic materials 0.000 claims description 4
- 239000011550 stock solution Substances 0.000 claims description 4
- 239000000835 fiber Substances 0.000 claims description 3
- 238000004108 freeze drying Methods 0.000 claims description 3
- YWYZEGXAUVWDED-UHFFFAOYSA-N triammonium citrate Chemical compound [NH4+].[NH4+].[NH4+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O YWYZEGXAUVWDED-UHFFFAOYSA-N 0.000 claims description 3
- 239000001393 triammonium citrate Substances 0.000 claims description 3
- 235000011046 triammonium citrate Nutrition 0.000 claims description 3
- 229920001817 Agar Polymers 0.000 claims description 2
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims description 2
- 235000007164 Oryza sativa Nutrition 0.000 claims description 2
- 229910019142 PO4 Inorganic materials 0.000 claims description 2
- 229920002472 Starch Polymers 0.000 claims description 2
- 239000008272 agar Substances 0.000 claims description 2
- 239000010977 jade Substances 0.000 claims description 2
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 claims description 2
- 239000010452 phosphate Substances 0.000 claims description 2
- 235000009566 rice Nutrition 0.000 claims description 2
- 238000005507 spraying Methods 0.000 claims description 2
- 230000007480 spreading Effects 0.000 claims description 2
- 239000008107 starch Substances 0.000 claims description 2
- 235000019698 starch Nutrition 0.000 claims description 2
- 230000008569 process Effects 0.000 abstract description 20
- 244000005700 microbiome Species 0.000 abstract description 10
- 230000008859 change Effects 0.000 abstract description 4
- 238000000855 fermentation Methods 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 3
- 238000013461 design Methods 0.000 abstract description 3
- 230000004151 fermentation Effects 0.000 abstract description 3
- 238000002386 leaching Methods 0.000 abstract description 3
- 238000005516 engineering process Methods 0.000 abstract description 2
- 229940039695 lactobacillus acidophilus Drugs 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 12
- 230000003203 everyday effect Effects 0.000 description 12
- 239000002054 inoculum Substances 0.000 description 8
- RWSOTUBLDIXVET-UHFFFAOYSA-N Dihydrogen sulfide Chemical compound S RWSOTUBLDIXVET-UHFFFAOYSA-N 0.000 description 6
- 229910021529 ammonia Inorganic materials 0.000 description 6
- 229910000037 hydrogen sulfide Inorganic materials 0.000 description 6
- 230000015556 catabolic process Effects 0.000 description 4
- 230000002354 daily effect Effects 0.000 description 4
- 238000006731 degradation reaction Methods 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 3
- 239000012153 distilled water Substances 0.000 description 3
- 238000010438 heat treatment Methods 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 235000013618 yogurt Nutrition 0.000 description 3
- 238000012935 Averaging Methods 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 229910000831 Steel Inorganic materials 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 238000002485 combustion reaction Methods 0.000 description 2
- 239000002361 compost Substances 0.000 description 2
- 230000001877 deodorizing effect Effects 0.000 description 2
- 239000010791 domestic waste Substances 0.000 description 2
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethanethiol Chemical compound CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 2
- 238000009422 external insulation Methods 0.000 description 2
- 238000001914 filtration Methods 0.000 description 2
- 238000009413 insulation Methods 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- 238000012946 outsourcing Methods 0.000 description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000011218 seed culture Methods 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000010959 steel Substances 0.000 description 2
- 239000004575 stone Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241000589220 Acetobacter Species 0.000 description 1
- QGZKDVFQNNGYKY-UHFFFAOYSA-O Ammonium Chemical compound [NH4+] QGZKDVFQNNGYKY-UHFFFAOYSA-O 0.000 description 1
- 206010002660 Anoxia Diseases 0.000 description 1
- 241000976983 Anoxia Species 0.000 description 1
- 241000193755 Bacillus cereus Species 0.000 description 1
- 241000222120 Candida <Saccharomycetales> Species 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 241001147746 Lactobacillus delbrueckii subsp. lactis Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000190932 Rhodopseudomonas Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000004099 anaerobic respiration Effects 0.000 description 1
- 230000007953 anoxia Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000004332 deodorization Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000004821 distillation Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005485 electric heating Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000010304 firing Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000002906 microbiologic effect Effects 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 239000003595 mist Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09B—DISPOSAL OF SOLID WASTE NOT OTHERWISE PROVIDED FOR
- B09B3/00—Destroying solid waste or transforming solid waste into something useful or harmless
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Mycology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Botany (AREA)
- Environmental & Geological Engineering (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a microbial agent for garbage composting as well as a preparation method and application thereof. The microbial agent comprises bacillus amyloliquefaciens, candida utilis, lactobacillus acidophilus and rhodopseudomonas palustris and is a liquid bacterial agent or solid bacterial agent. The microbial agent for garbage composting, disclosed by the invention, has the advantages of easiness in production, stable product quality, low cost and strong controllability in a garbage composting process; after the microbial agent is added into the garbage, the content of beneficial efficient microorganisms in the garbage can be increased, the microbial activity is improved, the garbage fermentation process is accelerated, the leaching of water and the increase of the lower calorific value of garbage are promoted, and the incineration efficiency of the garbage is improved; in a region with relatively low winter temperature, the microbial agent can reduce the influence caused by the low-temperature environment change so as to guarantee smooth operation of the garbage incineration according to the technology design; and by adopting the microbial agent, the generation of garbage smell can be reduced, and the working environment is improved.
Description
Technical field
The invention belongs to the biologic treating technique field of house refuse, particularly to a kind of microorganism for rubbish heap ferment
Microbial inoculum and its preparation method and application.
Background technology
The method of China's domestic rubbish disposal mainly has landfill method, composting process and burning method, landfill needs to take greatly at present
Amount soil, compost is low due to refuse classification degree, its product utilization difficulty, and burning method is in the minimizing, harmless of garbage disposal
Change and relative to other processing methods, there is sizable advantage with resource aspect.Along with the development of urbanization, can be used for filling life
The soil of rubbish alive reduces day by day, and incinerating method becomes increasing local selection in processing house refuse.But, I
There is the feature that moisture content is high, refuse thermal value is relatively low, China's major part domestic waste moisture content in the domestic waste of state
It is 40 ~ 70%.In the principal element affecting refuse thermal value, moisture content is the most notable on the impact of combustion heat value.Msw water content
The highest, the heat that during burning, water vapor is taken away is the most.According to Theoretical Calculation, 1kg water vapor needs to absorb the heat of about 2500kJ
Amount.Msw water content often reduces by 10% incinerating combustion value will improve 250KJ/kg.
Before waste incineration, in storage hole, house refuse is carried out heap ferment pretreatment is current China municipal solid waste incinerator
Common method.Rubbish carries out the stacking of a period of time in the garbage pool of stokehold, can ooze out moisture therein, improves into stove rubbish
The unit calorific value of rubbish.The moisture that rubbish leaches, in addition to the outside moisture by gravity extruding removing, mainly passes through microorganism
The combination moisture that organic Degradation is leached and other liquids.Rubbish heap ferment be different from rubbish compost, heap
Fertilizer is aerobic process, needs turning or ventilation, and heap ferment is mainly anoxia or anaerobic processes.Main work is played during rubbish heap ferment
Be microorganism anaerobic respiration and anaerobic fermentation.Microorganism, during heap ferment, destroys organic molecular structure, decomposes big
Molecular organic is more inflammable small-molecule substance, and release combines water, releases heat simultaneously and makes heap temperature rise, is more beneficial for micro-
Biological activity and moisture leach, thus improve calorific value.
Rubbish heap ferment is presently mainly nature heap ferment process, but the shortcoming of natural heap ferment is:
1, in natural heap ferment, micro organism quantity is few, and heap ferment process starts slow, and the moisture amount of leaching is few, and Lower heat value improves degree to be had
Limit.
2, natural heap ferment process is unstable, is easily affected by the external environment.Especially the northern area of China winter temperature is low, causes
The microbial activity carried in house refuse is extremely low, and within the heap ferment time of technological design, moisture removal amount is inadequate, rubbish low
Position calorific value reduces, and waste incineration efficiency declines.
3, natural heap ferment process is along with rubbish decay process, produces a large amount of stink, affects air quality.
Summary of the invention
It is an object of the invention to overcome the deficiency of rubbish nature heap ferment in prior art, it is provided that a kind of for rubbish heap
The microbial bacterial agent of the stability and high efficiency of ferment.
Another object of the present invention is to provide the preparation method of the above-mentioned microbial bacterial agent for rubbish heap ferment.
It is still another object of the present invention to provide the application of the above-mentioned microbial bacterial agent for rubbish heap ferment.
For achieving the above object, the present invention uses following technical proposals to be achieved:
A kind of microbial bacterial agent for rubbish heap ferment, it include bacillus amyloliquefaciens, Candida utilis, addicted to yogurt bar
Bacterium and Rhodopseudomonas palustris, described microbial bacterial agent is liquid microbe microbial inoculum or solid microbial, and described liquid is micro-
In bacteria agent, bacterial content is respectively bacillus amyloliquefaciens 2,000,000,000 ~ 5,000,000,000/milliliter, Candida utilis 3,000,000,000 ~ 6,000,000,000/in the least
Liter, bacillus acidophilus's 3,000,000,000 ~ 6,000,000,000/milliliter and Rhodopseudomonas palustris 2,000,000,000 ~ 5,000,000,000/milliliter；Described solid microbial
Middle bacterial content be respectively bacillus amyloliquefaciens 1,000,000,000 ~ 3,000,000,000/gram, Candida utilis 2,000,000,000 ~ 5,000,000,000/gram, addicted to yogurt bar
Bacterium 2,000,000,000 ~ 5,000,000,000/gram and Rhodopseudomonas palustris 1,000,000,000 ~ 3,000,000,000/gram.
Wherein, described bacillus amyloliquefaciens is the bacillus amyloliquefaciens of the CGMCC No.10011 that deposit number is
HFJ-7。
The invention provides the preparation method of the described liquid microbe microbial inoculum for rubbish heap ferment, including following step
Rapid:
(1) bacillus amyloliquefaciens, Candida utilis, bacillus acidophilus and Rhodopseudomonas palustris are cultivated at it respectively
Base is carried out cultivate and obtain bacterium solution；
(2) by described bacillus amyloliquefaciens bacterium solution: Candida utilis bacterium solution: bacillus acidophilus's bacterium solution: marsh is red false single
The ratio of born of the same parents bacterium bacterium solution 1 ~ 4:1 by volume ~ 5:1 ~ 5:1 ~ 3 is mixed with and obtains described liquid microbe microbial inoculum.
Present invention also offers the preparation method of the described solid microbial for rubbish heap ferment, specifically include down
State step:
(1) by bacillus amyloliquefaciens, Candida utilis, bacillus acidophilus and Rhodopseudomonas palustris respectively in correspondence
Culture medium activates, then cultivates respectively in respective culture medium and obtain bacterium solution；
(2) described bacillus amyloliquefaciens bacterium solution uses and is spray-dried, with Pulvis Talci for carrier be prepared as bacteria containing amount 10,000,000,000/gram ~
20000000000/gram powder, Candida utilis, bacillus acidophilus and Rhodopseudomonas palustris are respectively adopted lyophilization, with jade
Rice fiber powder be carrier make bacteria containing amount be 5,000,000,000 respectively/gram ~ 10,000,000,000/gram powder；
(3) bacillus amyloliquefaciens is pressed: Candida utilis: bacillus acidophilus: Rhodopseudomonas palustris=mass ratio 1 ~ 4:1 ~
The ratio of 10:1 ~ 10:1 ~ 5 is mixed and made into described solid microbial.
Further: described bacillus amyloliquefaciens is incubation time 24 ~ 48 hours at 30 DEG C；Described Candida utilis
Bacterium is incubation time 18 ~ 36 hours at 30 DEG C；Described bacillus acidophilus is incubation time 24 ~ 72 hours at 37 DEG C；Described marsh
Rhodopseudomonas is illumination cultivation time 100 ~ 150 hours at 30 DEG C.
Further: the culture medium of described bacillus amyloliquefaciens is with water as substrate, and pH7.0 ~ 7.2, including peptone
5.0g/L ~ 10.0g/L, Carnis Bovis seu Bubali cream 2.0g/L ~ 3.0g/L, glucose 10.0g/L ~ 20.0g/L, sodium chloride 3.0g/L ~ 5.0g/L；
Candida utilis culture medium with water as substrate, including: yeast extract 5.0g/L ~ 10.0g/L, peptone 10.0g/L ~
20.0g/L, glucose 10.0 ~ 20.0g/L；
Bacillus acidophilus's culture medium is with water as substrate, and pH 6.2～6.4, including peptone 5.0g/L ~ 10.0g/L, Carnis Bovis seu Bubali cream
5.0g/L ~ 10g/L, K2HPO4 1.0g/L ~ 2.0g/L, Triammonium citrate 1.0g/L ~ 2.0g/L, MgSO4·7H2O 0.6g/L~
1.0g/L, yeast extract 3.0g/L ~ 8.0g/L, sodium acetate 3.0g/L ~ 8.0g/L, glucose 15.0g/L ~ 30.0g/L,
Tween80 1.0ml/L ~ 3.0ml/L, CaCO33.0g ~ 8.0g/L, MnSO4·4 H2O 0.3g/L ~ 0.5g/L, agar
15.0g/L~20.0g/L；
Rhodopseudomonas palustris culture medium is with water as substrate, and pH 7.2-7.4, including sodium acetate 3.0g/L ~ 8.0g/L, peptone
1.5g/L ~ 2.0g/L, sodium chloride 0.5g/L ~ 1.5g/L, MgSO4·7H2O 0.2 g/L ~ 0.5g/L, NH4Cl 1.0g/L~
3.0g/L, K2HPO40.5g/L ~ 2.0g/L, Na2CO3 5.0g/L~8.0g/L。
Present invention also offers the application in the rubbish heap ferment before consumer waste incineration of the described microbial bacterial agent.
Further: the specifically used method of described liquid microbe microbial inoculum is: by liquid microbe microbial inoculum stock solution water
House refuse surface, rubbish spraying liquid microbial inoculum stock solution 0.25 ~ 5L per ton directly it is sprayed onto after 10 ~ 100 times of dilutions；Or by liquid
After the dilution of 10 ~ 100 times of body microbial bacterial agent use water, add after nutrient source spreads cultivation 12 ~ 72 hours under the conditions of 20 ~ 35 DEG C and be sprayed onto
House refuse surface, rubbish per ton sprays the bacterium solution 1 ~ 10L after spreading cultivation.
Further: the specifically used method of described solid microbial is: be directly spread to by solid microbial
In house refuse heap, mix homogeneously, rubbish per ton adds solid fungicide 0.5 ~ 5kg；Or solid microbial is used water 10-
After 100 times of dilutions, adding after nutrient source activates 12 ~ 72 hours under the conditions of 20 ~ 35 DEG C and be spread in house refuse heap, mixing is all
Even, rubbish per ton adds the microbial inoculum 1 ~ 10kg after activation.
Further: described nutrient source is brown sugar, carbamide, one or more in phosphate.
The present invention compared with prior art, has following prominent advantage and a beneficial effect:
1, the rubbish heap ferment microbial bacterial agent of the present invention is readily produced, constant product quality, and low cost is applied to rubbish heap ferment mistake
Journey controllability is strong.
2, after the microorganism formulation for rubbish heap ferment of the present invention adds in rubbish, by increasing capacitance it is possible to increase useful height in rubbish
The content of effect microorganism, improves microbial activity, accelerates the process of rubbish fermentation, promotes leaching and rubbish Lower heat value of moisture
Rising, improve rubbish firing efficiency.
3, the relatively low area of temperature in the winter time, uses this microbial bacterial agent can reduce low temperature environment and changes the impact brought,
Ensure that the burning of rubbish is smoothed out according to the design of technique.
4, the microbial bacterial agent of the present invention is used, it is also possible to reduce the generation of garbage odor, improve working environment.
Accompanying drawing explanation
Fig. 1 is that the embodiment of the present invention 5 is added before and after microbial inoculum waste incineration every day amount and works as daily temperature；
Fig. 2 is that the embodiment of the present invention 5 adds microbial inoculum forward backward averaging burning every day amount；
Fig. 3 is that the embodiment of the present invention 6 is added before and after microbial inoculum waste incineration every day amount and works as daily temperature；
Fig. 4 is that the embodiment of the present invention 6 adds microbial inoculum forward backward averaging burning every day amount.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described in further detail, but the embodiment of invention is not limited to this.
Embodiment 1
Microbial bacterial agent of the present invention includes bacillus amyloliquefaciens (Bacillus amyloloquefaciens), produces protein
The red false unit cell in candida mycoderma (Candida utilis), bacillus acidophilus (Lactobacillus acidophilus) and marsh
Bacterium (Rhodopseudanonas palustris), bacillus amyloliquefaciens of the present invention, Candida utilis, addicted to yogurt
Bacillus and Rhodopseudomonas palustris are selected from the microbial inoculum of market sale, and wherein said bacillus amyloliquefaciens also can use in being preserved in
The solution starch of the CGMCC No.10011 that the deposit number at state's Microbiological Culture Collection administration committee common micro-organisms center is
Bacillus cereus HFJ-7.
The depositary institution of described bacillus amyloliquefaciens HFJ-7: China Committee for Culture Collection of Microorganisms is the most micro-
Bio-Centers (CGMCC)；Address: Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, Institute of Microorganism, Academia Sinica；Preservation
Date: on November 19th, 2014；The deposit number of bacillus amyloliquefaciens Bacillus amyloliquefaciens is
CGMCC No. 10011。
The bacillus amyloliquefaciens of the present invention is the bacterial strain with organic matter degradation function, and Candida utilis is for having oil
The bacterial strain of fat degradation function, bacillus acidophilus is the bacterial strain with suppression putrefaction bacteria function, and Rhodopseudomonas palustris disappears for having
The bacterial strain of deodorization function.Four kinds of bacterial strains, by playing respective function, common degradation of organic substances in different phase, promote heap ferment mistake
The carrying out of journey, wherein Candida utilis and bacillus acidophilus are dominant bacteria at anaerobic stages.
The preparation method of microbial bacterial agent for rubbish heap ferment described in the present embodiment specifically includes following steps:
1, by bacillus amyloliquefaciens, Candida utilis, bacillus acidophilus and Rhodopseudomonas palustris respectively in correspondence
Culture medium activates, then the culture medium after respective sterilizing carries out seed culture.
The culture medium of described bacillus amyloliquefaciens consists of: peptone 10.0g, Carnis Bovis seu Bubali cream 3.0g, glucose 20.0g,
NaCl 5.0g, water 1000ml, pH7.0.Described bacillus amyloliquefaciens inoculum concentration is 10%, under the conditions of 30 DEG C, cultivates 22 little
Time.In the bacillus amyloliquefaciens bacterium solution obtained after cultivation, the bacteria containing amount of bacillus amyloliquefaciens is 30,000,000,000/milliliter.
The culture medium of described Candida utilis is: yeast extract 10.0g, peptone 20.0g, glucose 20.0g, distillation
Water 1000ml.Described Candida utilis inoculum concentration 4%, cultivates 24 hours under the conditions of 30 DEG C.The Candida utilis obtained after cultivation
In yeast liquid, the bacteria containing amount of Candida utilis is 10,000,000,000/milliliter.
Described bacillus acidophilus's culture medium: peptone 10.0g, Carnis Bovis seu Bubali cream 10.0g, yeast extract 5.0g, K2HPO4
2.0g, Triammonium citrate 2.0g, sodium acetate 5g, glucose 20g, Tween80 1ml, MgSO4·7H2O 0.58g, MnSO4·4
H2O 0.25g, CaCO35.0g, distilled water 1000ml, pH 6.2.Described bacillus acidophilus's inoculum concentration 4%, trains under the conditions of 37 DEG C
Support 24 hours.In the bacillus acidophilus's liquid obtained after cultivation, the bacteria containing amount of bacillus acidophilus is 10,000,000,000/milliliter.
Described Rhodopseudomonas palustris culture medium: sodium acetate 5.0g, peptone 1.5g, NaCl 0.5g, NH4Cl 1.0g
, MgSO4·7H2O 0.2 g, K2HPO40.5g, Na2CO35.0g, pH 7.4.Described Rhodopseudomonas palustris inoculum concentration 15%,
Under 2000lx ~ 3000lx illumination, cultivate 120 hours for 30 DEG C.In the Rhodopseudomonas palustris bacterium solution obtained after cultivation, marsh is red
The bacteria containing amount of pseudomonas is 10,000,000,000/milliliter.
2, above-mentioned cultivate by fermentation bacillus amyloliquefaciens liquid, Candida utilis liquid, bacillus acidophilus's liquid and
The ratio of Rhodopseudomonas palustris liquid 1:3:4:2 by volume is mixed and made into the microbial bacteria for rubbish heap ferment of the present invention
The liquid bacterial agent of agent, bacillus amyloliquefaciens content 3,000,000,000/milliliter in microbial inoculum, Candida utilis content 3,000,000,000/milliliter, addicted to
Lactobacillus lactis content 4,000,000,000/milliliter, Rhodopseudomonas palustris content 2,000,000,000/milliliter.
Embodiment 2
The preparation method of microbial bacterial agent for rubbish heap ferment described in the present embodiment specifically includes following steps:
1, by bacillus amyloliquefaciens, Candida utilis, bacillus acidophilus and Rhodopseudomonas palustris respectively in correspondence
Culture medium activates, then the culture medium after respective sterilizing carries out seed culture.
The culture medium of described bacillus amyloliquefaciens consists of: peptone 10.0g, Carnis Bovis seu Bubali cream 3.0g, glucose 20.0g,
NaCl 5.0g, water 1000ml, pH6.8.Bacillus amyloliquefaciens inoculum concentration is 8%, under the conditions of 30 DEG C, cultivates 24 hours.Training
In the bacillus amyloliquefaciens bacterium solution obtained after Yanging, the bacteria containing amount of bacillus amyloliquefaciens is 40,000,000,000/milliliter.
Described Candida utilis culture medium is: yeast extract 10.0g, peptone 20.0g, glucose 20.0g, distilled water
1000ml.Candida utilis inoculum concentration 5%, cultivates 18 hours under the conditions of 30 DEG C.The Candida utilis obtained after cultivation
In liquid, the bacteria containing amount of Candida utilis is 10,000,000,000/milliliter
Described bacillus acidophilus's culture medium: peptone 10g, Carnis Bovis seu Bubali cream 10g, yeast extract 5g, K2HPO4 2.0g, citric acid three
Ammonium 2.0g, sodium acetate 5.0g, glucose 20.0g, Tween80 1ml, MgSO4·7H2O 0.58g, MnSO4·4 H2O
0.25g, CaCO35.0g, distilled water 1000ml, pH 6.4.Bacillus acidophilus's inoculum concentration 3%, cultivates 24 little under the conditions of 37 DEG C
Time.In the bacillus acidophilus's liquid obtained after cultivation, the bacteria containing amount of bacillus acidophilus is 10,000,000,000/milliliter.
Described Rhodopseudomonas palustris culture medium: sodium acetate 5g, peptone 1.5g, NaCl 0.5g, NH4Cl 2.0g,
MgSO4 ·7H2O 0.2 g, K2HPO40.5g, Na2CO35.0g, PH 7.2.Rhodopseudomonas palustris inoculum concentration 10%,
Under 2000lx illumination, cultivate 120 hours for 28 DEG C.Rhodopseudomonas palustris in the Rhodopseudomonas palustris bacterium solution obtained after cultivation
Bacteria containing amount is 10,000,000,000/milliliter.
2, the 1.0L bacillus amyloliquefaciens bacterium solution after cultivating adds 1.0kg Pulvis Talci, after mix homogeneously, use spray
Mist is dried makes pressed powder, again add Pulvis Talci be prepared as bacteria containing amount 20,000,000,000/gram microbial inoculum.Institute the most after incubation
State the corn fiber powder adding 1.0kg sterilizing in Candida utilis, bacillus acidophilus and Rhodopseudomonas palustris 1.0L bacterium solution,
After mix homogeneously, under the conditions of-50 DEG C, vacuum lyophilization obtains solid fungicide, again add carrier make bacteria containing amount 5,000,000,000/gram
Microbial inoculum.
3, according to bacillus amyloliquefaciens microbial inoculum: Candida utilis microbial inoculum: bacillus acidophilus's microbial inoculum: marsh is red false single
The solid fungicide that born of the same parents bacterium solid fungicide=mass ratio 1:4:6:2 is mixed is rubbish heap ferment solid microbial.
Embodiment 3
Gathering the house refuse in the refuse collection station of resident living area, Qingdao City, after artificial broken bag, mix homogeneously is stand-by.Life
The physical composition (wet basis mass fraction) of rubbish be foodstuff 74.1%, stationery 11.5%, plastics 4.3%, other 10.1%.It is initial
Water content (wet basis) is 66.2%.Processing in rustless steel container, container height 120cm, internal diameter 40cm, outsourcing 10cm are thick
Hollow cotton pad insulation, bottom pad 10 cm thick stone collects percolate as filtering layer, by 50kg(wet basis) aforementioned house refuse
Being respectively charged in 6 experimental provisions, wherein 3 experimental provisions add embodiment 1 according to the ratio that quantity of refuse mass ratio is 0.1%
The described microbial bacterial agent prepared, other 3 experimental provisions spray mass ratio be the water of 0.1% as blank, each device
In, rubbish adds loads 10kg.Sample analysis after processing 3 days, data are shown in Table 1.From table 1 it follows that add at microbial inoculum
Msw water content after reason reaches 48.3%, reduces by 8.4% than control treatment, and Lower heat value reaches 6024kJ/kg, ratio comparison place
Reason increases 1235kJ/kg, adds the rubbish after microbial inoculum processes and more suitably burns.
Table 1 msw water content and calorific value
| Msw water content (%) | Lower heat value (kJ/kg) | |
| Initial waste | 66.2±3.51 | 4053±127 |
| Add microbial inoculum rubbish | 48.3±2.88 | 6024±339 |
| Comparison | 56.7±5.04 | 4789±302 |
Embodiment 4
Gathering the house refuse in the refuse collection station of resident living area, Qingdao City, after artificial broken bag, mix homogeneously is stand-by.Life
The physical composition (wet basis mass fraction) of rubbish be foodstuff 78.2%, stationery 13.5%, plastics 4.4%, other 3.9%.It is initial
Water content (wet basis) is 67.3%.Processing in rustless steel container, container height 120cm, internal diameter 40cm, outsourcing 10cm are thick
Hollow cotton pad insulation, bottom pad 10 cm thick stone collects percolate as filtering layer, by 50kg(wet basis) aforementioned house refuse
Being respectively charged in 6 experimental provisions, wherein the rubbish of 3 experimental provisions adds and accounts for the embodiment 2 that rubbish mass ratio is 0.2% and make
Described microbial bacterial agent, other 3 as blank, in each device, rubbish adds loads 10kg.Process 3 days
Rear sample analysis, data are shown in Table 2.Reduce from Table 2, it can be seen that add the msw water content after microbial inoculum processes than control treatment
6.7% reaches 47.4%, and Lower heat value reaches 6105kJ/kg, increases 1117kJ/kg, the rubbish after microbial inoculum process than control treatment
Rubbish more suitably burns.
Table 2 msw water content and calorific value
| Msw water content (%) | Calorific value (kJ/kg) | |
| Initial waste | 67.3±3.64 | 4121±139 |
| Add microbial inoculum rubbish | 47.4±3.32 | 6105±324 |
| Comparison | 54.1±4.93 | 4988±279 |
Embodiment 5
In December, 2015 Shandong fixed-end forces factory, the rubbish heap ferment time is 7 days, is not added with microbial inoculum first 14 days, and average every day burns
Burning amount is 170.64 tons.Microbial inoculum activation and spray appliance are set in rubbish heap ferment workshop.
Microbial inoculum activation equipment: 2 500L buckets, as activation culture container numbering 1# respectively, 2#, are respectively connected to electric heating tube
Heated for controlling temperature, and be incubated by external insulation measure.2 buckets are made to be in an activation culture, in 1 use state, every angel
By 1 barrel of bacterium solution, again cultivate after use.
Activation method: in the bucket of 500L, adds brown sugar 10kg；Carbamide 0.5kg；Potassium dihydrogen phosphate 0.1kg；Water 200L；
After heating for dissolving, water temperature drops to about the 30 DEG C microbial inoculum 20kg added in embodiment 2, keeps water temperature 25-30 DEG C activation training
Use after supporting 1 day.
Arranging microbial inoculum spray appliance in rubbish heap ferment workshop, sanitation cart is often discharged into a collection of rubbish, according to accounting in rubbish heap
Rubbish mass ratio is the microbial bacterial agent of the present invention of the embodiment 2 after the ratio interpolation activation of 0.2%, runs 14 days continuously,
Before and after interpolation microbial inoculum, waste incineration amount and temperature Change on the same day are as shown in Figure 1.When ground temperature is relatively low, but when Daily minimum temperature with
Maximum temperature difference before and after adding microbial inoculum is little, and after adding microbial inoculum, the burning amount of rubbish is increased dramatically.Add microbial inoculum
After 14 days, burn away the refuse average every day 196.00 tons, with untreated before compared with, average waste incineration every day amount improve
14.86%, as shown in Figure 2.Data above proves, adds described microbial inoculum and can improve rubbish and burn in the case of temperature is relatively low in the winter time
Burn the waste incineration amount of factory's every day.
Stench intensity, ammonia concentration, concentration of hydrogen sulfide and TVOC concentration, result in heap ferment workshop before and after mensuration processes simultaneously
Such as table 3.Ammonia in the air of heap ferment space, hydrogen sulfide, the concentration of TVOC the most greatly reduces, and reduces by 67.12% respectively than before,
57.53%, 49.19%, odor strength reduces by 55.33%, and heap ferment environment is substantially improved.
Table 3 adds deodorizing effect before and after microbial inoculum
| Testing index | Before process | After process | Reduction rate (%) |
| Odor strength (OU) | 11253 | 5027 | 55.33 |
| Ammonia concentration (mg/m3) | 7.3 | 2.4 | 67.12 |
| Concentration of hydrogen sulfide (mg/m3) | 14.6 | 6.2 | 57.53 |
| TVOC concentration (mg/m3) | 12.4 | 6.3 | 49.19 |
Embodiment 6
2016 02 month Shandong fixed-end forces factory, the rubbish heap ferment time is 7 days, and average burning every day amount is 167.36 tons,
Rubbish heap ferment workshop arranges microbial inoculum activation and atomized spray equipment.
Microbial inoculum activation equipment: 3 500L buckets, as activation culture container numbering 1# respectively, 2#, 3#, are respectively connected to electrical heating
Pipe heated for controlling temperature, and be incubated by external insulation measure.Make 3 buckets be in two bacterium liquid activation to cultivate, in 1 use state,
Use 1 barrel of bacterium solution every day, again cultivate after use.
Activation method: in the bucket of 500L, adds brown sugar 10kg；Carbamide 0.5kg；Potassium dihydrogen phosphate 0.1kg；Water 200L；
After heating for dissolving, water temperature drops to about the 30 DEG C microbial inoculum 20L added in embodiment 1, seals and keeps water temperature 25-30 DEG C activation
Use after cultivating 2 days.
Sanitation cart is often discharged into a collection of rubbish, starts device and sprays, and rubbish per ton uniformly sprays the bacterium solution that 1L activation culture is good.
Running 14 days continuously, before and after interpolation microbial inoculum, waste incineration amount and temperature Change on the same day are as shown in Figure 3.Local when Daily minimum temperature with
Maximum temperature difference before and after adding microbial inoculum is little, and after adding microbial inoculum, the burning amount of rubbish is increased dramatically.Add microbial inoculum
After 14 days, burn away the refuse average every day 202.73 tons, with untreated before compared with, average waste incineration every day amount improve
21.14%, as shown in Figure 4.Result proves, adds this microbial inoculum and can improve garbage burning factory every in the case of temperature is relatively low in the winter time
The waste incineration amount of day.
Mensuration process before and after stench intensity, ammonia concentration, concentration of hydrogen sulfide and TVOC concentration, measurement result in heap ferment workshop
Such as table 4 below.Ammonia in the air of heap ferment space, hydrogen sulfide, the concentration of TVOC reduces by 67.94% respectively than comparison, and 47.82%,
50.43%, odor strength reduces by 53.87% than before, and heap ferment environment is substantially improved.
Table 4 adds deodorizing effect before and after microbial inoculum
| Testing index | Before process | After process | Reduction rate (%) |
| Odor strength (OU) | 10253 | 4729 | 53.87 |
| Ammonia concentration (mg/m3) | 7.8 | 2.5 | 67.94 |
| Concentration of hydrogen sulfide (mg/m3) | 11.5 | 6.0 | 47.82 |
| TVOC concentration (mg/m3) | 11.5 | 5.7 | 50.43 |
Above-mentioned enforcement example is the preferred embodiment of the present invention, but embodiments of the present invention are not limited by above-described embodiment
System, amendment that other any is made without departing from the principle of the invention and essence, modifies, replaces, simplifies, combine, all should be equivalent
Substitute mode, within being included in protection scope of the present invention.
Claims (10)
1. the microbial bacterial agent for rubbish heap ferment, it is characterised in that: it includes bacillus amyloliquefaciens, Candida utilis ferment
Female bacterium, bacillus acidophilus and Rhodopseudomonas palustris, described microbial bacterial agent is liquid microbe microbial inoculum or solid microbe bacterium
Agent, in described liquid microbe microbial inoculum, bacterial content is respectively bacillus amyloliquefaciens 2,000,000,000 ~ 5,000,000,000/milliliter, Candida utilis
Bacterium 3,000,000,000 ~ 6,000,000,000/milliliter, bacillus acidophilus's 3,000,000,000 ~ 6,000,000,000/milliliter and Rhodopseudomonas palustris 2,000,000,000 ~ 5,000,000,000/milliliter；Described
In solid microbial bacterial content be respectively bacillus amyloliquefaciens 1,000,000,000 ~ 3,000,000,000/gram, Candida utilis 2,000,000,000 ~ 50
Hundred million/gram, bacillus acidophilus 2,000,000,000 ~ 5,000,000,000/gram and Rhodopseudomonas palustris 1,000,000,000 ~ 3,000,000,000/gram.
Microbial bacterial agent for rubbish heap ferment the most according to claim 1, it is characterised in that: described solution starch spore bar
Bacterium is the bacillus amyloliquefaciens HFJ-7 of the CGMCC No.10011 that deposit number is.
3. the preparation method of the liquid microbe microbial inoculum for rubbish heap ferment described in claim 1, it is characterised in that under including
State step:
(1) bacillus amyloliquefaciens, Candida utilis, bacillus acidophilus and Rhodopseudomonas palustris are cultivated at it respectively
Base is carried out cultivate and obtain bacterium solution；
(2) by described bacillus amyloliquefaciens bacterium solution: Candida utilis bacterium solution: bacillus acidophilus's bacterium solution: marsh is red false single
The ratio of born of the same parents bacterium bacterium solution 1 ~ 4:1 by volume ~ 5:1 ~ 5:1 ~ 3 is mixed with and obtains described liquid microbe microbial inoculum.
4. the preparation method of the solid microbial for rubbish heap ferment described in claim 1, it is characterised in that specifically wrap
Include following step:
(1) by bacillus amyloliquefaciens, Candida utilis, bacillus acidophilus and Rhodopseudomonas palustris respectively in correspondence
Culture medium activates, then cultivates respectively in respective culture medium and obtain bacterium solution；
(2) described bacillus amyloliquefaciens bacterium solution uses and is spray-dried, with Pulvis Talci for carrier be prepared as bacteria containing amount 10,000,000,000/gram ~
20000000000/gram powder, Candida utilis, bacillus acidophilus and Rhodopseudomonas palustris are respectively adopted lyophilization, with jade
Rice fiber powder be carrier make bacteria containing amount be 5,000,000,000 respectively/gram ~ 10,000,000,000/gram powder；
(3) bacillus amyloliquefaciens is pressed: Candida utilis: bacillus acidophilus: Rhodopseudomonas palustris=mass ratio 1 ~ 4:1 ~
The ratio of 10:1 ~ 10:1 ~ 5 is mixed and made into described solid microbial.
The preparation method of the solid microbial for rubbish heap ferment the most according to claim 4, it is characterised in that: institute
State bacillus amyloliquefaciens incubation time 24 ~ 48 hours at 30 DEG C；Described Candida utilis is incubation time at 30 DEG C
18 ~ 36 hours；Described bacillus acidophilus is incubation time 24 ~ 72 hours at 37 DEG C；Described Rhodopseudomonas palustris is at 30 DEG C
100 ~ 150 hours illumination cultivation time.
The preparation method of the solid microbial for rubbish heap ferment the most according to claim 4, it is characterised in that: institute
State the culture medium of bacillus amyloliquefaciens with water as substrate, pH7.0 ~ 7.2, including: peptone 5.0g/L ~ 10.0g/L, Carnis Bovis seu Bubali cream
2.0g/L ~ 3.0g/L, glucose 10.0g/L ~ 20.0g/L, sodium chloride 3.0g/L ~ 5.0g/L；
Candida utilis culture medium with water as substrate, including: yeast extract 5.0g/L ~ 10.0g/L, peptone 10.0g/L ~
20.0g/L, glucose 10.0 ~ 20.0g/L；
Bacillus acidophilus's culture medium is with water as substrate, and pH 6.2～6.4, including peptone 5.0g/L ~ 10.0g/L, Carnis Bovis seu Bubali cream
5.0g/L ~ 10g/L, K2HPO4 1.0g/L ~ 2.0g/L, Triammonium citrate 1.0g/L ~ 2.0g/L, MgSO4·7H2O 0.6g/L~
1.0g/L, yeast extract 3.0g/L ~ 8.0g/L, sodium acetate 3.0g/L ~ 8.0g/L, glucose 15.0g/L ~ 30.0g/L,
Tween80 1.0ml/L ~ 3.0ml/L, CaCO33.0g ~ 8.0g/L, MnSO4·4 H2O 0.3g/L ~ 0.5g/L, agar
15.0g/L~20.0g/L；
Rhodopseudomonas palustris culture medium is with water as substrate, and pH 7.2-7.4, including sodium acetate 3.0g/L ~ 8.0g/L, peptone
1.5g/L ~ 2.0g/L, sodium chloride 0.5g/L ~ 1.5g/L, MgSO4·7H2O 0.2 g/L ~ 0.5g/L, NH4Cl 1.0g/L~
3.0g/L, K2HPO40.5g/L ~ 2.0g/L, Na2CO3 5.0g/L~8.0g/L。
7. the application in the rubbish heap ferment before consumer waste incineration of the microbial bacterial agent described in claim 1.
The microbial bacterial agent the most according to claim 7 application in the rubbish heap ferment before consumer waste incineration, its
It is characterised by: the specifically used method of described liquid microbe microbial inoculum is: by 10 ~ 100 times of liquid microbe microbial inoculum stock solution water
House refuse surface, rubbish spraying liquid microbial inoculum stock solution 0.25 ~ 5L per ton directly it is sprayed onto after dilution；Or by liquid microbe
After the dilution of 10 ~ 100 times of microbial inoculum use water, add after nutrient source spreads cultivation 12 ~ 72 hours under the conditions of 20 ~ 35 DEG C and be sprayed onto house refuse
Surface, rubbish per ton sprays the bacterium solution 1 ~ 10L after spreading cultivation.
The microbial bacterial agent the most according to claim 7 application in the rubbish heap ferment before consumer waste incineration, its
It is characterised by: the specifically used method of described solid microbial is: solid microbial is directly spread to house refuse
In heap, mix homogeneously, rubbish per ton adds solid fungicide 0.5 ~ 5kg；Or by dilute for 10-100 times of solid microbial water
After releasing, add after nutrient source activates 12 ~ 72 hours under the conditions of 20 ~ 35 DEG C and be spread in house refuse heap, mix homogeneously, rubbish per ton
Rubbish adds the microbial inoculum 1 ~ 10kg after activation.
The answering in the rubbish heap ferment before consumer waste incineration of microbial bacterial agent the most according to claim 8 or claim 9
With, it is characterised in that: described nutrient source is brown sugar, carbamide, one or more in phosphate.
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| CN111004748A (en) * | 2019-12-26 | 2020-04-14 | 青岛尚德生物技术有限公司 | Microbial strengthening microbial inoculum capable of remarkably promoting fermentation effect before household garbage incineration and application thereof |
| CN111040968A (en) * | 2019-12-26 | 2020-04-21 | 青岛根源生物技术集团有限公司 | Microbial agent for efficiently removing COD (chemical oxygen demand) in domestic sewage and application thereof |
| CN111004748B (en) * | 2019-12-26 | 2021-01-26 | 青岛尚德生物技术有限公司 | Microbial strengthening microbial inoculum capable of remarkably promoting fermentation effect before household garbage incineration and application thereof |
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