CN111040968A - Microbial agent for efficiently removing COD (chemical oxygen demand) in domestic sewage and application thereof - Google Patents
Microbial agent for efficiently removing COD (chemical oxygen demand) in domestic sewage and application thereof Download PDFInfo
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
Abstract
The invention discloses a microbial agent for efficiently removing COD (chemical oxygen demand) in domestic sewage and application thereof, wherein the microbial agent is prepared by mixing mixed bacterial powder and a carrier, wherein the mixed bacterial powder contains 1-5 hundred million CFU/g of Lactobacillus parvus with the preservation number of CGMCC No.18391, 1-5 hundred million CFU/g of Clostridium butyricum with the preservation number of CGMCC No.14499, 10-50 hundred million CFU/g of Bacillus lentus with the preservation number of CGMCC No.18392 and 10-50 hundred million CFU/g of Bacillus psychrophilus with the preservation number of CGMCC No. 18393. The microbial agent disclosed by the invention has the capability of remarkably degrading COD in domestic sewage, can be used for preparing a preparation for removing COD in domestic sewage of cities and towns, effectively solves the problem that the COD of effluent does not reach the standard in the sewage treatment process, and ensures the stable operation of a sewage treatment system.
Description
Technical Field
The invention belongs to the technical field of environmental microorganism application engineering, and particularly relates to a microbial agent for efficiently removing COD (chemical oxygen demand) in domestic sewage and application thereof.
Background
COD content in sewage is always an important index for measuring whether water quality reaches the standard in various sewage treatment processes, and is also an important component of domestic sewage. The content of COD in the domestic sewage generally fluctuates within the range of 200-500mg/L according to different water quality sources, and the emission standard generally requires below 60 mg/L. In the domestic sewage treatment process, COD is removed to a great extent by means of degradation of microorganisms, and particularly, the microorganisms play a vital role in the treatment of rural domestic sewage.
In addition, biological treatment of sewage is widely used for treating various kinds of waste water, and biochemical systems are also essential components of the overall system for treating various kinds of sewage. The enhanced microbial agent with specific functions can not only promote the quick start of a new sewage system, but also play an important role and value in helping to recover system problems caused by increased sewage treatment load, sludge aging and the like, so that the search for the microbial agent which can play a role in removing COD in domestic sewage is very important.
Disclosure of Invention
In order to efficiently remove COD in domestic sewage and ensure that the quality of effluent after sewage treatment reaches the standard, the invention provides a microbial agent for efficiently removing COD in domestic sewage and application thereof.
The invention provides a microbial agent for efficiently removing COD (chemical oxygen demand) in domestic sewage, which comprises mixed bacterial powder and a carrier; the mixed bacterium powder comprises lactobacillus parafarraginis with bacterium content of 1-5 hundred million CFU/g, clostridium butyricum with bacterium content of 1-5 hundred million CFU/g, bacillus lentus with bacterium content of 10-50 hundred million CFU/g and psychrophilic bacillus with bacterium content of 10-50 hundred million CFU/g.
Further, the lactobacillus paradise adopts a preservation number of: lactobacillus paracasei GBW-HB1903 of CGMCC No. 18391.
Further, the bacillus lentus adopts a preservation number as follows: bacillus lentus GBW-HB1902 with CGMCC No. 18392.
Further, the bacillus psychrophilus adopts a preservation number of: bacillus psychrophilus GBW-HB1901 of CGMCC No. 18393.
Further, the clostridium butyricum adopts a preservation number of: clostridium butyricum GBW-N1 with CGMCC No. 14499.
Further, the preparation method of the microbial agent comprises the following steps:
(1) respectively carrying out activated culture on bacillus lentus, bacillus psychrophilus, lactobacillus pahnsonii and clostridium butyricum in a culture medium to obtain bacterial liquids of all bacteria;
(2) mixing the Bacillus lentus liquid and the Bacillus psychrophilus liquid in an equal volume ratio, adding a carrier (the unit of the liquid is L, the unit of the carrier is kg) with a volume-mass ratio of 1: 1-2, spray-drying at a tower temperature of 80-85 ℃ and an air-out temperature of 75-80 ℃ to obtain dried bacterial powder, and adding the carrier with a mass ratio of 1: 1-2 into the dried bacterial powder to obtain mixed bacterial powder A;
(3) mixing clostridium butyricum bacterial liquid and lactobacillus paradoxus bacterial liquid in an equal volume ratio, adding a carrier (the unit of the bacterial liquid is L, and the unit of the carrier is kg) with a volume-mass ratio of 1: 1-2, carrying out vacuum freeze drying at-45 to-50 ℃ to obtain dried bacterial powder, and adding the carrier with a mass ratio of 1: 4-5 into the dried bacterial powder to obtain mixed bacterial powder B;
(4) and uniformly mixing the mixed bacterium powder A and the mixed bacterium powder B according to the mass ratio of 1: 1-5 to obtain the microbial agent.
Further, the temperature of the vacuum freeze drying is-45 to-50 ℃.
Further, the carrier is at least one of corn flour, glucose, starch, talcum powder and calcium carbonate.
The invention also provides application of the microbial agent in preparation of a preparation for efficiently removing COD (chemical oxygen demand) in domestic sewage.
Furthermore, the addition amount of the microbial inoculum is 0.1-0.5 per mill of the volume of the domestic sewage.
Further, the domestic sewage comprises urban domestic sewage and town domestic sewage.
Further, the specific use method of the microbial preparation is as follows: before use, the microbial inoculum is diluted by 20-100 times by water, then a nutrient source is added, aeration or stirring activation is carried out for 12-48 h at 20-35 ℃, then the mixture is poured into an aerobic pool of a sewage treatment system, internal circulation debugging is carried out for 24-72h, and then the normal operation state of the system is gradually recovered.
Further, the nutrient source comprises at least one of molasses, glucose, brown sugar, corn steep liquor, urea, ammonium chloride and phosphate.
Furthermore, the microbial agent can obviously degrade the COD concentration in the domestic sewage.
Compared with the prior art, the invention has the following advantages and beneficial effects:
the microbial agent can obviously degrade COD in domestic sewage so as to reduce the concentration of the COD, is controllable in production, stable in product quality and low in cost, and has strong operability when being applied to a field domestic sewage biochemical treatment system.
The psychrophilic bacillus and the retarded bacillus in the microbial agent can grow rapidly under the low-temperature aerobic condition in winter, and the lactobacillus paracasei and the clostridium butyricum can rapidly utilize COD (chemical oxygen demand) components in a sewage system to carry out growth and metabolism under the anaerobic condition with insufficient aerobic or aeration quantity, so that under the low-temperature, normal-temperature, aerobic or anaerobic conditions, a plurality of microorganisms can complement and cooperate with each other, a sewage treatment system can be started rapidly, COD in domestic sewage can be removed efficiently, and the long-term stable operation of the system can be guaranteed.
After the microbial agent is applied to a sewage biochemical treatment system, the abundance and the content of original functional microorganisms in the system can be supplemented, the microbial activity is improved, the stability of the biochemical system is improved, the degradation of COD in the sewage system is accelerated, the impact resistance of the system is enhanced, the COD concentration of effluent is reduced, and the quality of the effluent is ensured to reach the standard, so that the problem that the COD concentration of the effluent does not reach the standard due to the impact of the system or the aging of sludge and the like is solved, and therefore, the microbial agent has a wide application prospect.
Drawings
FIG. 1 is the colony morphology characteristics of the Lactobacillus parvum GBW-HB1903 on MRS plates.
FIG. 2 depicts the morphological characteristics of colonies of Bacillus lentus GBW-HB1902 on 2216E plates.
FIG. 3 depicts the colony morphology of Bacillus psychrophilus GBW-HB1901 on 2216E plates.
FIG. 4 is the effect of the microbial inoculum on COD concentration in municipal wastewater.
FIG. 5 shows the effect of the microbial inoculum on COD concentration in rural domestic sewage.
FIG. 6 is the effect of the microbial inoculum on COD concentration in a field application debugging test of rural domestic sewage.
FIG. 7 is a graph showing the effect of the microbial inoculum on COD concentration in a field application commissioning test of municipal domestic sewage.
Detailed Description
The present invention will be described in further detail with reference to the following specific examples.
Example 1
Separation, screening and identification of Lactobacillus parvus GBW-HB1903
1. Screening of Lactobacillus parvum GBW-HB1903
Municipal domestic sewage is used as a sample, after gradient dilution, a calcium carbonate-containing MRS culture medium is adopted for pouring culture and screening a bacterial colony with an obvious calcium-dissolving ring, a single bacterial colony is obtained after multiple purification, and the single bacterial colony is named as GBW-HB1903 and is stored.
As shown in figure 1, bacterial colony of the bacterial strain GBW-HB1903 on an MRS plate is round, milky, 1-2 μm in diameter, smooth, moist and glossy in surface, slightly convex in middle, non-transparent, neat in edge and free of halo, the bacterial strain GBW-HB1903 can normally grow and propagate within the temperature range of 10-45 ℃, the optimal growth temperature is 25-35 ℃, the optimal growth pH value is 6.5-7.5, the bacterial strain GBW-HB1903 can normally grow within the range of dissolved oxygen content of less than 0.5mg/L or more than 1.0mg/L, the optimal dissolved oxygen content is 2-3mg/L, the bacterial strain GBW-HB1903 belongs to facultative anaerobes, the bacterial strain GBW-HB1903 can produce urease, β -glucosidase and glycerol, and can degrade or decompose kinetic nitrate, simon-type citrate and semisolid agar.
2. 16S rRNA sequencing of Lactobacillus parvus GBW-HB1903
And (3) amplifying the 16S rRNA universal primer by using the DNA of the strain GBW-HB1903 as a template, and performing sequence determination on the amplified fragment. The 16S rDNA sequencing result of GBW-HB1903 is compared with the sequence in GenBank for analysis, and the result shows that the strain GBW-HB1903 has the highest homology with Lactobacillus parafarraginis, so that the strain GBW-HB1903 is determined to be Lactobacillus parvum.
And (3) performing strain preservation on the screened strain GBW-HB1903, wherein the preservation unit of the Lactobacillus parvus GBW-HB1903 is as follows: china general microbiological culture Collection center (CGMCC); address: western road No.1, north west city of township, beijing, institute of microbiology, china academy of sciences; the preservation date is as follows: year 2019, month 08, day 16; the preservation number of the Lactobacillus parfarraginis is CGMCC No. 18391.
II, separation, screening and identification of Bacillus lentus GBW-HB1902
1. Screening and purification of Bacillus lentus GBW-HB1902
And (3) taking the municipal landfill leachate as bacterial suspension for sample culture, carrying out gradient dilution, then carrying out streak culture on a 2216E solid culture medium, carrying out multiple separation and purification to obtain a single bacterial colony, and storing the single bacterial colony, wherein the single bacterial colony is named as GBW-HB 1902.
As shown in figure 2, the bacterial colony of the bacterial strain GBW-HB1902 on a 2216E plate is circular, light yellow, 1-2 mu m in diameter, smooth, moist and glossy in surface, slightly convex in the middle, non-transparent, neat in edge and free of halo, the bacterial strain GBW-HB1902 can normally grow at the temperature of 15-40 ℃, the optimal growth temperature is 28-32 ℃, the bacterial strain can grow at the pH value of 6-9, the optimal growth pH value is 7.2-8.5, the tolerance to an acidic environment is poor, the bacterial strain GBW-HB1902 can normally grow at the dissolved oxygen content of 1-5 mg/L, the optimal growth dissolved oxygen concentration is 2-4mg/L, urease, β -glucosidase and glycerol can be produced, the dynamic nitrate, the simon-type citrate and semisolid agar can be degraded or decomposed, the bacterial strain GBW-HB1902 has broad salt property, and can well grow at the salinity of 20-40 per thousand.
2. Identification of Bacillus lentus GBW-HB1902
The DNA of the strain GBW-HB1902 is used as a template, 16S rRNA universal primers are used for amplification, sequence determination is carried out on amplified fragments, the 16S rDNA sequencing result of the obtained strain GBW-HB1902 is compared with the sequence in GenBank for analysis, and the result shows that the strain GBW-HB1902 has the highest homology with Bacillus lentus, so that the strain GBW-HB1902 is determined to be Bacillus lentus.
The strain GBW-HB1902 is selected and subjected to strain preservation, and the preservation unit of the Bacillus lentus GBW-HB1902 is as follows: china general microbiological culture Collection center (CGMCC); address: western road No.1, north west city of township, beijing, institute of microbiology, china academy of sciences; the preservation date is as follows: year 2019, month 08, day 16; the preservation number of the Bacillus lentus is CGMCC No. 18392.
Screening, separating and identifying Bacillus psychrophilus GBW-HB1901
1. Separation, screening and purification of psychrophilic bacillus GBW-HB1901
Taking seawater and domestic sewage collected in winter environment as samples, carrying out streak culture on a 2216E solid culture medium after gradient dilution on a cultured bacterial suspension, separating and purifying for multiple times to obtain a single bacterial colony, and storing the single bacterial colony named as GBW-HB 1901.
As shown in figure 3, a bacterial colony of the bacterial strain GBW-HB1901 on a 2216E plate is round, light yellow, 1-2 mu m in diameter, smooth, moist and glossy in surface, slightly convex in middle, opaque, neat and serrated in edge and free of halo, the bacterial strain GBW-HB1901 can grow in a pH range of 6-9, the optimal growth pH value is 6.5-7.5, the bacterial strain can normally grow in a dissolved oxygen content range of 1-5 mg/L, the optimal growth dissolved oxygen concentration is 2-4mg/L, urease, β -glucosidase and glycerol can be produced, power-nitrate, simon citrate and semisolid agar can be degraded or decomposed, the bacterial strain can normally grow and propagate in a temperature range of 5-25 ℃, and the optimal growth temperature is 18-22 ℃.
2. Identification of Bacillus psychrophilus GBW-HB1901
The DNA of the strain GBW-HB1901 is used as a template, a 16S rRNA universal primer is used for amplification, the sequence of an amplified fragment is determined, the 16S rDNA sequencing result of the obtained strain GBW-HB1901 is compared with the sequence in GenBank for analysis, and the result shows that the strain GBW-HB1901 has the highest homology with Bacillus psychrophilus, so that the strain GBW-HB1901 is determined to be the Bacillus psychrophilus.
And (3) performing strain preservation on the screened strain GBW-HB1901, wherein the preservation unit of the psychrophilic bacillus GBW-HB1901 is as follows: china general microbiological culture Collection center (CGMCC); address: western road No.1, north west city of township, beijing, institute of microbiology, china academy of sciences; the preservation date is as follows: year 2019, month 08, day 16; the preservation number of the Bacillus psychrophilus is CGMCC No. 18393.
Screening, separation and identification of Clostridium butyricum GBW-N1
1. Separation, screening and purification of clostridium butyricum GBW-N1
And (3) carrying out streak culture on the bacterial suspension cultured by the collected sample on a 2216E solid culture medium after gradient dilution, separating and purifying for multiple times to obtain a single bacterial colony, namely GBW-N1, and storing.
The bacterial colony of the bacterial strain GBW-N1 is round, milky, smooth in surface, slightly convex in middle and irregular in edge, the bacterial strain GBW-N1 can grow in the range of pH 6-8, the optimal growth pH value is 7.1-7.5, the bacterial strain GBW-N1 can normally grow in the range of dissolved oxygen content of 1-5 mg/L, the optimal growth dissolved oxygen concentration is 2-4mg/L, urease, β -glucosidase and glycerol can be produced, power-nitrate, simon citrate and semisolid agar can be degraded or decomposed, the bacterial colony can normally grow and propagate in the temperature range of 30-45 ℃, and the optimal growth temperature is 35-38 ℃.
2. Identification of Clostridium butyricum GBW-N1
The DNA of the strain GBW-N1 is used as a template, 16S rRNA universal primers are used for amplification, sequence determination is carried out on amplified fragments, the 16S rDNA sequencing result of the obtained strain GBW-N1 is compared with the sequence in GenBank, and the result shows that the strain GBW-N11 has the highest homology with Clostridium butyricum, so that the strain GBW-N1 is determined to be Clostridium butyricum.
And (3) performing strain preservation on the screened strain GBW-N1, wherein the preservation unit of the clostridium butyricum GBW-N1 is as follows: china general microbiological culture Collection center (CGMCC); address: western road No.1, north west city of township, beijing, institute of microbiology, china academy of sciences; the preservation date is as follows: year 2017, month 08, day 07; the preservation number of the clostridium butyricum is CGMCC No. 14499.
Example 2
The preparation method of the microbial agent for efficiently removing COD in domestic sewage comprises the following steps:
1. lactobacillus parvum, Clostridium butyricum, Bacillus lentus and Bacillus psychrophilus are activated in respective media and then cultured in the respective media.
The culture medium formula and culture conditions of the lactobacillus paradisi, the clostridium butyricum, the bacillus lentus and the bacillus psychrophilus are as follows:
(1) culture medium of lactobacillus paradise: 25g of corn steep liquor dry powder, 5g of yeast extract and K2HPO43g, 1.5g of triammonium citrate and MgSO4·7H20.3g of O, 2g of sodium acetate, 50g of molasses, 801 ml of Tween and CaCO35g，MnSO4·4H20.1g of O, 1000ml of distilled water, pH 6.5, the culture time of 22h and the culture temperature of 35 ℃.
(2) Clostridium butyricum culture medium: 50g of glucose, 20g of corn steep liquor dry powder, 3g of yeast extract, 2g of sodium acetate, 1g of sodium chloride and MgSO40.1g，K2HPO41.5g，Na2CO32g, 1000ml of distilled water, pH 7.2, incubation time 20h, incubation temperature 32 ℃.
(3) B, a bacillus lentus culture medium: 25g of soybean peptone, 3g of yeast extract, 40g of glucose, 2g of sodium chloride, 1000ml of water, pH 7.0, culture time of 60h and culture temperature of 35 ℃.
(4) Psychrophilic bacillus culture medium: 20g of soybean peptone, 7g of yeast extract, 30g of glucose, 4g of sodium chloride, 1000ml of water, pH 7.2, culture time of 64h and culture temperature of 20 ℃.
2. 1.0kg of calcium carbonate is added into 1.0L of cultured bacillus lentus and bacillus psychrophilus mixed bacteria liquid with the volume ratio of 1:1, after uniform mixing, the mixture is prepared into bacteria powder by adopting a spray drying mode under the conditions that the temperature in a tower is 82 ℃ and the air outlet temperature is 78 ℃, and then 1kg of carrier is added again to prepare mixed bacteria powder A with the bacteria content of 1300 hundred million CFU/g.
3. 1.0kg of sterilized talcum powder is added into 1.0L of cultured mixed bacterial liquid of clostridium butyricum and lactobacillus paradise with the volume ratio of 1:1, the mixture is uniformly mixed and then is subjected to vacuum freeze drying at the temperature of minus 45 ℃ to obtain bacterial powder, and 5kg of carrier is added again to prepare mixed bacterial powder B with the bacterial content of 800 hundred million CFU/g.
4. And finally, mixing the obtained mixed bacterial powder of the lactobacillus paschenii, the clostridium butyricum, the bacillus lentus and the bacillus psychrophilus according to the proportion of the final bacterial concentration ratio of 1:1:5:5 to prepare the microbial agent.
The bacteria content of the lactobacillus paradisi, the clostridium butyricum, the bacillus lentus and the bacillus psychrophilus in the microbial agent is 1-5 hundred million CFU/g, 10-50 hundred million CFU/g and 10-50 hundred million CFU/g respectively.
In this embodiment, the carrier is at least one of corn flour, glucose, starch, talc and calcium carbonate.
Example 3
The method comprises the steps of collecting domestic sewage to be treated in a municipal domestic sewage treatment plant in urban areas of Qingdao city, equally and separately loading the sewage into 12 wide-mouth beakers of 2L, wherein each beaker is separately loaded with 1.5L of domestic sewage and totally divided into 6 groups, each group is 2 in parallel, a CK group is a blank control group without microbial inoculum, other test conditions are the same as those of test groups, the test groups are A1, A2, A3, A4 and A5 groups, and the addition amount of microbial inoculum corresponding to each group is 0.1 thousandth, 0.2 thousandth, 0.3 thousandth, 0.4 thousandth and 0.5 thousandth respectively. The initial COD concentration in each test group sample is subjected to sample retention detection before the test is started, the pH value of each group is controlled to be 6-9, each group is subjected to aeration treatment by using a small aerator with the same model, the dissolved oxygen in water of each group is controlled to be not less than 2mg/L, the pH value and the COD concentration in each group sample are subjected to tracking detection at a fixed time every day, and the whole test period is 7 days. The test results are shown in fig. 4, each test group can obviously reduce the COD concentration compared with a blank control group, and the microbial agent test groups with different addition amounts have no obvious difference, which indicates that under the condition of lower addition amount, the microbial agent provided by the invention has the capability of obviously reducing the COD concentration in municipal domestic sewage and degrading the COD in the domestic sewage.
Example 4
Collecting domestic sewage to be treated in a village and town domestic sewage collection point in Weifang city, equivalently dividing the sewage into 12 wide-mouth beakers of 2L, dividing each beaker into 6 groups of 1.5L domestic sewage, wherein each group is 2 in parallel, CK group is a blank control group without microbial inoculum, other test conditions are the same as those of test groups, the test groups are B1, B2, B3, B4 and B5 groups, and the addition amount of microbial inoculum corresponding to each group is 0.1 thousandth, 0.2 thousandth, 0.3 thousandth, 0.4 thousandth and 0.5 thousandth respectively. The initial COD concentration in each test group sample is subjected to sample retention detection before the test is started, the pH value of each group is controlled to be 6-9, each group is subjected to aeration treatment by using a small aerator with the same model, the dissolved oxygen in water of each group is controlled to be not less than 2mg/L, the pH value and the COD concentration in each group sample are subjected to tracking detection at a fixed time every day, and the whole test period is 7 days. The test results are shown in fig. 5, each test group can significantly reduce the COD concentration compared with the blank control group, and there is no significant difference between the microbial agent test groups with different addition amounts, which indicates that under the condition of lower addition amount, the microbial agent provided by the invention has the ability of significantly reducing the COD concentration in domestic sewage from villages and towns and degrading the COD in the domestic sewage.
Example 4
The microbial agent product is combined with an actual engineering case for application verification, and aiming at the debugging test of integrated sewage treatment equipment in Weihai, a certain country and the like, the basic process flow of the treatment system is as follows: a water collecting well, a septic tank and integrated treatment equipment (anaerobic treatment, two-section aerobic treatment and settling); at present, the rural domestic sewage treatment project is debugged and operated, but the treatment system has the problem that the effluent water does not reach the standard all the time, and the effluent water mainly reaches the COD concentration which does not reach the standard, the COD concentration of the inlet water of the system before the experiment is 280 plus 300mg/L, and the COD concentration of the outlet water is 100 plus 120mg/L, which is far higher than the required COD concentration standard of the outlet water (the COD concentration is less than or equal to 60 mg/L).
Before the test is started, various operation indexes of the treatment system are maintained and detected, dissolved oxygen in aerobic section water is guaranteed to be not less than 2mg/L, the pH value is controlled to be 6-9, and the microbial agent is added according to the addition amount of 0.1 per mill. Before the microbial agent is used, diluting the microbial agent by 20-100 times with water, adding a nutrient source, aerating or stirring and activating for 12-48 h at 20-35 ℃, then pouring the microbial agent into an aerobic pool of a sewage treatment system, carrying out internal circulation debugging for 24-72h by matching with the actual situation of a field process, and then gradually recovering the normal running state of the system; the COD concentration of the settled and discharged water end is tracked and detected at a fixed time every day, and the whole test period is 7 days. Wherein the nutrient source is at least one of molasses, glucose, brown sugar, corn steep liquor, urea, ammonium chloride and phosphate.
The test result is shown in fig. 6, in the first 4 days of adding the microbial inoculum, the COD concentration of the effluent in the sewage treatment system begins to rapidly decrease, then the COD degradation rate is slowed down along with the decrease of the COD concentration in the system, and from the 4 th day of debugging, the COD concentration of the effluent of the treatment system is already lower than 60mg/L required by the standard, and then 3 days are all stabilized within the standard, thus successfully completing the field application debugging test of the microbial inoculum for rural domestic sewage.
Example 5
The microbial agent product is combined with an actual engineering case for application verification, and the application debugging test of a biochemical system in a certain municipal sewage treatment plant in the cigarette platform urban area is aimed atThe basic process flow is as follows: primary sedimentation tank + coarse grid + fine grid + A2O + secondary sedimentation tank + disinfection tank; at present, the domestic sewage treatment system faces the problem that the COD concentration of the effluent water does not reach the standard, the COD concentration of the inlet water of the system before the experiment is 380-400mg/L, the COD concentration of the effluent water is 110-120mg/L, and is far higher than the COD concentration standard of the required effluent water (the COD concentration is less than or equal to 50 mg/L).
And (3) inspecting and detecting various system operation parameters before the test starts, ensuring that the dissolved oxygen in the aerobic section is not less than 2mg/L, controlling the pH value to be 6-9, and adding the microbial inoculum according to the use amount of 0.1 per mill. Before the microbial agent is used, diluting the microbial agent by 20-100 times with water, adding a nutrient source, aerating or stirring and activating for 12-48 h at 20-35 ℃, then pouring the microbial agent into an aerobic pool of a sewage treatment system, carrying out internal circulation debugging for 24-72h by matching with the actual situation of a field process, and then gradually recovering the normal running state of the system; and (4) tracking and detecting the COD concentration of the water outlet end of the secondary sedimentation tank at a fixed time every day, wherein the whole test period is 7 days.
The test result is shown in fig. 7, after 7 days of field application test debugging, the concentration of the COD of the effluent of the sewage treatment system begins to rapidly decrease within the first 4 days of adding the microbial inoculum, then is maintained below 40mg/L, and from the 4 th day of debugging, the concentration of the COD of the effluent of the system is already lower than 50mg/L required by the standard, and the field application debugging test of the microbial inoculum for the urban domestic sewage is successfully completed.
In conclusion, both indoor test and field application evaluation results show that the microbial agent can obviously degrade COD in domestic sewage and reduce COD concentration, and has strong system adaptability, quick start and high efficiency; in addition, the production process of the microbial agent is mature and stable, the operation is simple and convenient, the microbial agent is convenient to use, is suitable for sewage treatment in various places, accords with market application expectation, and has industrial value.
The above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that various changes may be made and equivalents may be substituted for elements thereof; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions.
Claims (10)
1. A microbial agent for efficiently removing COD in domestic sewage is characterized in that: the microbial agent comprises mixed bacterial powder and a carrier; the mixed bacterium powder comprises lactobacillus parafarraginis with bacterium content of 1-5 hundred million CFU/g, clostridium butyricum with bacterium content of 1-5 hundred million CFU/g, bacillus lentus with bacterium content of 10-50 hundred million CFU/g and psychrophilic bacillus with bacterium content of 10-50 hundred million CFU/g.
2. The microbial agent for efficiently removing COD in domestic sewage according to claim 1, wherein the Lactobacillus paracasei adopts the collection number: lactobacillus paracasei GBW-HB1903 of CGMCC No. 18391.
3. The microbial agent for efficiently removing COD in domestic sewage according to claim 1, wherein said Bacillus lentus is Bacillus lentus with the deposit number: bacillus lentus GBW-HB1902 with CGMCC No. 18392.
4. The microbial agent for efficiently removing COD in domestic sewage according to claim 1, wherein said Bacillus psychrophilus is selected from Bacillus psychrophilus according to the following preservation number: bacillus psychrophilus GBW-HB1901 of CGMCC No. 18393.
5. The microbial agent for efficiently removing COD in domestic sewage according to claim 1, wherein said Clostridium butyricum has the following preservation number: clostridium butyricum GBW-N1 with CGMCC No. 14499.
6. The microbial agent for efficiently removing COD in domestic sewage according to claim 1, wherein the preparation method of the microbial agent comprises:
(1) respectively carrying out activated culture on the bacillus lentus, the psychrophilic bacillus, the lactobacillus pahnsonii and the clostridium butyricum in a culture medium to obtain a bacillus lentus bacterial liquid, a bacillus psychrophilic bacterial liquid, a lactobacillus pahnsonii bacterial liquid and a clostridium butyricum bacterial liquid;
(2) mixing the Bacillus lentus liquid and the Bacillus psychrophilus liquid in the step (1) in an equal volume ratio, adding a carrier with a volume-mass ratio of 1: 1-2, performing spray drying to obtain dry bacterial powder, and adding the carrier with a mass ratio of 1: 1-2 into the dry bacterial powder to obtain mixed bacterial powder A;
(3) mixing the clostridium butyricum bacterial liquid and the lactobacillus parvum bacterial liquid in the step (1) in an equal volume ratio, adding a carrier with a volume-to-mass ratio of 1: 1-2, carrying out vacuum freeze drying to obtain dried bacterial powder, and adding the carrier with a mass ratio of 1: 4-5 into the dried bacterial powder to obtain mixed bacterial powder B;
(4) and (3) uniformly mixing the mixed bacterium powder A in the step (2) and the mixed bacterium powder B in the step (3) according to a mass ratio of 1: 1-5 to obtain the microbial agent.
7. The microbial agent for efficiently removing COD in domestic sewage according to claim 1 or 6, wherein said carrier is at least one of corn flour, glucose, starch, talcum powder and calcium carbonate.
8. Use of the microbial inoculant according to any one of claims 1 to 7 for the preparation of a formulation for efficient removal of COD in domestic wastewater.
9. The use of claim 8, wherein the microbial inoculum is added in an amount of 0.1 to 0.5% by volume of the domestic wastewater.
10. The use according to claim 8, wherein the microbial preparation is used by: diluting the microbial agent by 20-100 times with water, adding a nutrient source, aerating or stirring and activating, and pouring into a sewage treatment system for use.
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