CN105861302B - The uniform illumination device of algae culture based on aquatile ecotoxicity assessment - Google Patents
The uniform illumination device of algae culture based on aquatile ecotoxicity assessment Download PDFInfo
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- CN105861302B CN105861302B CN201610218578.3A CN201610218578A CN105861302B CN 105861302 B CN105861302 B CN 105861302B CN 201610218578 A CN201610218578 A CN 201610218578A CN 105861302 B CN105861302 B CN 105861302B
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- web plate
- aquatile
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- illumination device
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
Abstract
The invention discloses a kind of uniform illumination device of the algae culture based on aquatile ecotoxicity assessment, including incubator body, the incubator body interior has been arranged on automatic temperature control system；Some web plate layers being parallel to each other are provided with by arrangement for adjusting height in the incubator body；The web plate layer be lower floor's web plate and upper strata web plate be arranged in parallel composition internal cavity structure stratum reticulare, the upper strata web plate upper surface of the web plate layer, which is uniformly arranged, sets some alloy cross bars vertical with incubator body, uniformly some strip-shaped light sources of arrangement setting, the set location and quantity of the strip-shaped light source are corresponding with alloy cross bar on lower floor's web plate inside the web plate layer.The present invention provides a kind of uniform illumination device of the algae culture based on aquatile ecotoxicity assessment, controls the illumination condition in Acute Toxicity of Algae, and the culture for making to be in incubator receives the efficient light sources of equality strength.
Description
Technical field
The uniform illumination device of algae culture of the invention based on aquatile ecotoxicity assessment, is mainly used in algae etc.
The culture of biology belongs to ecological and ecological toxicology field for ecotoxicity assessment.
Background technology
In the ecological risk assessment and forecasting research of environmental monitoring and chemicals, only lean on to the chemical detection of pollutant without
Method practically reflects the influence for being discharged into the chemical pollutant of water body to the ecosystem, the research of aquatile ecotoxicity assessment
It is to predict step essential in chemicals risk.Wherein, the acute toxicity test of algae is the most commonly used standard method
One of, provide scientific basis to formulate environmental criteria and discharge standard.In the method, in the reliability of toxicity assessment result
In quality control index, it is desirable to which during toxicity test is carried out, algal grown need to be under unified experiment condition.Illumination is algae
Most important condition in class growth, algal grown speed therefore are being tested by the influence of the factor such as power, the uniformity of light of illumination
During require strict.Traditional illumination box of market sale, light source come from incubator body upper part or the left and right sides,
Culture cell to placing wherein diverse location, can not provide the illumination of equality strength, generally closer to incubator interposition
Put, its intensity of illumination is weaker, or even the intensity of illumination for not reaching algal grown requirement is simultaneously, comes from incubator top or left and right
The light source of both sides, to the culture among incubator, the uniformity for receiving illumination around it is also not quite similar.
The content of the invention
Goal of the invention：In order to overcome the deficiencies in the prior art, the present invention provides a kind of based on aquatile ecology
The uniform illumination device of the algae culture of toxicity assessment, the illumination condition in Acute Toxicity of Algae is controlled, make to be in incubator
Culture receive the efficient light sources of equality strength.
Technical scheme：To achieve the above object, technical scheme is as follows：
The uniform illumination device of algae culture based on aquatile ecotoxicity assessment, including incubator body, it is described
Incubator body interior has been arranged on automatic temperature control system；It is provided with the incubator body by arrangement for adjusting height some
The web plate layer being parallel to each other；The web plate layer be lower floor's web plate and upper strata web plate be arranged in parallel composition internal cavity Structure Network
Layer, the upper strata web plate upper surface of the web plate layer, which is uniformly arranged, sets some alloy cross bars vertical with incubator body, described
Uniformly arrangement sets some strip-shaped light sources on lower floor's web plate inside web plate layer, the set location and quantity of the strip-shaped light source with
Alloy cross bar is corresponding.
Further, some strip-shaped light sources are connected with plug wire insert row respectively, and the plug wire insert row is connected with timer.
Further, the spacing between the adjacent alloy cross bar is equal to the bottom for placing the triangular flask being wherein fixedly installed
Diameter.
Further, the spacing between the adjacent strip-shaped light source is straight more than or equal to the bottom for the triangular flask being placed on it
Footpath.
Further, the strip-shaped light source includes trichromatic fluorescent light tube and the fluorescent light support with shutter；It is described
Fluorescent light support with shutter for upper shed diverging light induce strip bracket groove, and with the setting of alloy cross bar
Direction is consistent；The trichromatic fluorescent light tube is correspondingly arranged at the inside middle of each fluorescent light support with shutter.
Further, the fluorescent light support includes bottom plate and two pieces of shutters, two pieces of shutters respectively with bottom
Plate is be hinged to be set, and pulls the relative angle between shutter regulation and bottom plate.
Beneficial effect：The present invention is growth based on the algae evaluated for acute toxicity and requirement of experiment and proposes,
Compared with existing technology, the technology has the advantage that：
1st, strip-shaped light source is provided by fluorescent tube, lower section of the fluorescent tube inside every layer of web plate layer, can be shone directly into
The bottom of the culture vessel of glass material, change above incubator or the light source of the left and right sides in incubator light it is strong
Not the shortcomings that degree does not reach requirement of experiment.
2nd, the fluorescent tube as light source is parallel and equidistant installation, parallel between alloy cross bar and apart from equal, ensures
Biology left and right between alloy cross bar is prevented unilateral light is excessive from causing the unilateral growth of algae by the illumination of equality strength
Sheng is crossed, that is, grows uneven phenomenon.
3rd, the relative position of alloy cross bar and light source is fixed, ensure that between any two alloys cross bar biology by
The synthesis illumination of same intensity, while fluorescent tube, towards upwards, the support on the downside of fluorescent tube has light-proofness, prevents upper strata web plate
Light source be irradiated to lower floor, eliminate influence of the uneven illumination to intensity of illumination difference between Duplicate Samples.
4th, it is serially connected between fluorescent tube, lamp luminescence state is in same level, eliminates power supply shakiness or fluorescent tube
The even influence of uneven illumination caused by damage.The trichromatic fluorescent light tube of not homology class can be selected as needed simultaneously, using with
Convenient disassembly.
Brief description of the drawings
Accompanying drawing 1 is the front view of structure of the present invention；
Accompanying drawing 2 is connection diagram of the present invention；
Accompanying drawing 3 is that web plate layer of the present invention, fluorescent tube and alloy cross bar arrange schematic diagram；
Accompanying drawing 4 is the adjacent fluorescent tube illumination analysis chart of the present invention；
Accompanying drawing 5 is the top view of web plate Rotating fields part of the present invention.
Embodiment
The present invention is further described below in conjunction with the accompanying drawings.
In the present embodiment, for use as chlorella vulgaris (the chlorella vulgaris, FACHB- of acute toxicity test
8), designed according to acute toxicity test requirement and the growth of chlorella vulgaris and propagation.
Such as accompanying drawing 1,2,3 and 5, the uniform illumination device of the algae culture based on aquatile ecotoxicity assessment, including
Incubator body 1, it is made up of opaque material, chamber door is made up of transparent material (such as glass), and transparent chamber door is convenient at any time
Chlorella vulgaris growing state is observed, chamber door and casing can form closed space.It has been arranged on inside the incubator body 1
Automatic temperature control system；Some web plate layers 4 being parallel to each other are provided with by arrangement for adjusting height in the incubator body 1, can
The height and spacing of web plate are adjusted according to actual conditions；The web plate layer 4 is that lower floor's web plate and upper strata web plate be arranged in parallel composition
Internal cavity structure stratum reticulare, the upper strata web plate upper surface of the web plate layer 4 uniformly arranges to set some hangs down with incubator body 1
Straight alloy cross bar 43, uniformly arrangement sets some strip-shaped light sources on lower floor's web plate inside the web plate layer 4, light source towards to
On, the set location and quantity of the strip-shaped light source are corresponding with alloy cross bar 43.Illumination is sent by the bottom up of web plate layer 4,
The intensity of light is ensure that, while to placing triangular flask therein, is mainly provided by the fluorescent light from triangular flask or so
Same intensity illumination such as Fig. 4, so as to controlling the intensity of light and the uniformity of light well, eliminate uneven illumination to flat
The influence of intensity of illumination difference between row sample, ensure that the validity of laboratory test results.
Some strip-shaped light sources are connected with plug wire insert row 3 respectively, and the plug wire insert row 3 is connected with timer.
Spacing between the adjacent alloy cross bar 43 is equal to the base diameter for placing the triangular flask being wherein fixedly installed.Phase
Spacing between the adjacent strip-shaped light source is more than or equal to the base diameter for the triangular flask being placed on it.
The strip-shaped light source includes trichromatic fluorescent light tube 42 and the fluorescent light support 41 with shutter；The band blocks
The fluorescent light support 41 of plate for upper shed diverging light induce strip bracket groove, and with the setting side of alloy cross bar 43
To consistent；The trichromatic fluorescent light tube 42 is correspondingly arranged at the inside center of each fluorescent light support 41 with shutter
Between.
In this example, by the use of China of state 250-D types illumination box as incubator body, culture the temperature inside the box is by culture
The temperature-controlling system control that case carries, according to incubator setting program, the whole light sources that incubator carries are opened, and install and filled
The web plate layer of trichromatic fluorescent light tube.When timer is closed, the fluorescent tube that light source is all carried by incubator provides, now according to
The setting of alloy cross bar, the intensity of illumination in the region everywhere that 5 alloy cross bars are formed is measured respectively.As a result such as following table：(light intensity list
Position：Lux)
| 1 | 2 | 3 | 4 | Average | Standard deviation |
| 1620.00 | 720.00 | 980.00 | 1387.00 | 1176.75 | 403.32 |
Close incubator automatic light source, opening timing device, now light source all carried by web plate bottom trichromatic fluorescent light tube
For in identical, 4 points measure intensity of illumination, as a result such as following table：
| 1 | 2 | 3 | 4 | Average | Standard deviation |
| 2530.00 | 2660.00 | 2580.00 | 2537.00 | 2576.75 | 59.74 |
The measurement result of light intensity is shown：The mean intensity of light can be effectively improved by the present invention in incubator,
Standard deviation is greatly lowered simultaneously, shows that the uniformity of light can be obviously improved simultaneously.Illustrate that the invention can be realized to interior
Effective control of illumination condition is tested, is eliminated due to influence caused by excessive light differential, it is ensured that the acute toxicity tests
Reliability.
Further, in order to realize more uniform illumination, the fluorescent light support 41 includes bottom plate and two pieces block
Plate, two pieces of shutters with the be hinged setting of bottom plate, pull the relative angle between shutter regulation and bottom plate respectively, preferable real
Testing the intensity of illumination standard deviation of data can be contracted within 50.
Described above is only the preferred embodiment of the present invention, it should be pointed out that：For the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (5)
1. the uniform illumination device of the algae culture based on aquatile ecotoxicity assessment, it is characterised in that：Including incubator
Body (1), the incubator body (1) are internally provided with automatic temperature control system；Adjusted in the incubator body (1) by height
Regulating device is provided with some web plate layers (4) being parallel to each other；The web plate layer (4) is that lower floor's web plate and upper strata web plate be arranged in parallel
The structure stratum reticulare of the internal cavity of composition, the upper strata web plate upper surface of the web plate layer (4), which is uniformly arranged, sets some and incubator
The vertical alloy cross bar (43) of the front and rear table plane of body (1), uniformly arrangement is set on the internal lower floor's web plate of the web plate layer (4)
Some strip-shaped light sources are put, the set location and quantity of the strip-shaped light source are corresponding with alloy cross bar (43)；The strip-shaped light source bag
Include trichromatic fluorescent light tube (42) and the fluorescent light support (41) with shutter；The fluorescent light support with shutter
(41) strip bracket groove is induced for the light of upper shed diverging, and it is consistent with the setting direction of alloy cross bar (43)；It is described
Trichromatic fluorescent light tube (42) is correspondingly arranged at the inside middle of each fluorescent light support (41) with shutter.
2. the uniform illumination device of the algae culture according to claim 1 based on aquatile ecotoxicity assessment, it is special
Sign is：Some strip-shaped light sources are connected with plug wire insert row (3) respectively, and the plug wire insert row (3) is connected with timer.
3. the uniform illumination device of the algae culture according to claim 1 based on aquatile ecotoxicity assessment, it is special
Sign is：Spacing between the adjacent alloy cross bar (43) is equal to the base diameter for placing the triangular flask being wherein fixedly installed.
4. the uniform illumination device of the algae culture according to claim 1 based on aquatile ecotoxicity assessment, it is special
Sign is：Spacing between the adjacent strip-shaped light source is more than or equal to the base diameter for the triangular flask being placed on it.
5. according to the uniform illumination device of the algae culture based on aquatile ecotoxicity assessment described in claim 1 or 4, its
It is characterised by：The fluorescent light support (41) includes bottom plate and two pieces of shutters, and two pieces of shutters are cut with scissors with bottom plate respectively
Setting is connect, pulls the relative angle between shutter regulation and bottom plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610218578.3A CN105861302B (en) | 2016-04-08 | 2016-04-08 | The uniform illumination device of algae culture based on aquatile ecotoxicity assessment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610218578.3A CN105861302B (en) | 2016-04-08 | 2016-04-08 | The uniform illumination device of algae culture based on aquatile ecotoxicity assessment |
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| Publication Number | Publication Date |
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| CN105861302A CN105861302A (en) | 2016-08-17 |
| CN105861302B true CN105861302B (en) | 2018-02-13 |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106841371A (en) * | 2017-04-12 | 2017-06-13 | 东南大学 | A kind of method that utilization flight time mass spectrum differentiates Cells of Blue-green Algae |
| CN107245441A (en) * | 2017-06-29 | 2017-10-13 | 天津大学 | A kind of microalgae culture system with ventilatory function and multi-faceted adjustable illumination |
| CN111704992B (en) * | 2020-07-09 | 2023-04-11 | 中国水产科学研究院珠江水产研究所 | Adjustable algae experimental device based on environment change |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN201268702Y (en) * | 2008-06-18 | 2009-07-08 | 中国科学技术大学 | High evenness light irradiation biochemical cultivation case |
| CN101701183B (en) * | 2009-10-28 | 2012-07-18 | 福建师范大学 | Isolated cell photodynamic action effect reference apparatus based on LED array |
| CN102144548A (en) * | 2011-01-01 | 2011-08-10 | 司红康 | Plant tissue culture shelf and light supplement method thereof |
| CN102337204B (en) * | 2011-10-02 | 2013-07-03 | 海南大学 | Light/temperature dual-gradient microalgae incubator |
| CN102613077A (en) * | 2012-03-27 | 2012-08-01 | 山东纯英光电科技有限公司 | Elevating LED adjustable-light culture rack |
| CN102787069B (en) * | 2012-08-09 | 2013-08-21 | 重庆大学 | Diatom growth variable research system based on square diatom culture frame |
| CN103275871B (en) * | 2013-06-13 | 2014-12-24 | 天津开发区合普工贸有限公司 | Detecting device used in phototoxicity experiment of cells in perforated plates |
| JP6169423B2 (en) * | 2013-06-28 | 2017-07-26 | 株式会社日本医化器械製作所 | Algae culture lighting device |
| CN203741322U (en) * | 2014-02-25 | 2014-07-30 | 中国石油大学(华东) | Microalgae culture oscillator bracket with light source |
| CN203916699U (en) * | 2014-04-22 | 2014-11-05 | 张秋丰 | A kind of biological culture frame for biological toxicity test |
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