CN102337204B - Light/temperature dual-gradient microalgae incubator - Google Patents
Light/temperature dual-gradient microalgae incubator Download PDFInfo
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- CN102337204B CN102337204B CN 201110294544 CN201110294544A CN102337204B CN 102337204 B CN102337204 B CN 102337204B CN 201110294544 CN201110294544 CN 201110294544 CN 201110294544 A CN201110294544 A CN 201110294544A CN 102337204 B CN102337204 B CN 102337204B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/14—Incubators; Climatic chambers
Abstract
The invention relates to a light/temperature dual-gradient microalgae incubator. The incubator comprises an incubator body (1), wherein an insulation layer (2) is arranged outside the incubator body, a refrigeration zone (3) and a heating zone (8) are respectively arranged at the left and right sides of the incubator body, a working zone (5) is arranged in the middle of the incubator body, a refrigerator and a temperature controller are arranged in the refrigeration zone, and a heating rod and the temperature controller are arranged in the heating zone; metal grids (6) are uniformly distributed in the working zone (5), and a heat conduction medium (9) is arranged in the metal grids; a plurality of cold light lamps (7) are uniformly fixed and arranged in the same plane above the working zone (5) from less to more; and the arrangement direction of the cold light lamps is vertical to the directions of the refrigeration zone and the heating zone. The light/temperature dual-gradient microalgae incubator has simple structure; and by using a light and temperature dual-gradient control mode, selection of algal species and optimization of culture conditions can be carried out in an effective, rapid and high-flux manner, thus experimental microalgae light/temperature composite experiment data can be completed once, research and development period is shortened, research and development process is simplified, and an effective way is provided for research and development of algae.
Description
Technical field
The invention belongs to the biological culture field, relate to a kind of biological culture device, specifically is a kind of little algae incubator of light/temperature dual-gradient that carries out illumination, the optimization of temperature culture condition.
Background technology
Little algae is that a class grows in the unicellular or many cells microphyte in seawater or the fresh water, has important research and using value.The research of little algae and application need filter out advantage algae kind, because the advantage algae kind that filters out under different temperature and illumination condition is all inequality, therefore, the optimization of the illumination of little algae and temperature culture condition is the indispensable prerequisite of algae resource development and utilization: under differing temps and the illumination condition, can screen and obtain different advantage algae kinds; Different algae kinds have the suitableeest illumination and a temperature range, and the acquisition of this basic data is to carry out the prerequisite that little algae produces and studies.
At present, the optimization of the light of little algae/warm culture condition is that the suitableeest illumination of little algae, the selection of temperature condition are to carry out many wheel experiments to obtain in the temperature controller device, each takes turns the culture cycle that the required time of experiment is little algae (static cultivation needs about 20 days), therefore the optimization of light/warm culture condition of little algae is an extremely loaded down with trivial details and consumption time-consuming job, in addition, many wheel experiments have increased experimental error inevitably, reduce the accuracy of experimental result, thereby influence the optimum result of light/warm culture condition of little algae.With regard to current experiments equipment, manpower and time conditions, traditional method can not satisfy the needs of little algae biological experiment and the research and development of little algae.
Summary of the invention
The objective of the invention is provides a kind of light/temperature dual-gradient little algae incubator at the deficiencies in the prior art, the mode of employing illumination, the two gradients controls of temperature quick, the high-throughout algae kind of carrying out is effectively screened and culture condition optimization, reduce experiment labour intensity and experimental error, realize the little algae light of experiment, disposable the finishing of warm complication experiment data, simplify the R﹠D process of algae.
The technical solution adopted in the present invention:
The little algae incubator of a kind of light/temperature dual-gradient, comprise casing, the outer setting thermal insulation layer of casing, the left and right sides at casing arranges refrigeration area and heating zone respectively, the middle part is workspace, heat transfer efficiencies preferred metal dividing plate is set respectively on the relative position of refrigeration area and heating zone, refrigerator and thermostat are set in the refrigeration area, can freeze and keep the homo(io)thermism of refrigeration area under the lower temperature condition, heating rod and thermostat are set in the heating zone, can heat and keep the homo(io)thermism of heating zone under the higher temperatures condition; Workspace evenly arranges metal grill at the middle part of casing, namely adopt the heat transfer efficiencies preferred metal to be made into the identical grid of specification size, in metal grill, be provided with heat-conduction medium, two ends in casing make a temperature end and a low-temperature end thus, temperature head appears between the two ends, heat conducts naturally by the heat-conduction medium in the middle of the two ends, is changed continuously by high temperature to low temperature, thereby form continuous thermograde in metal grill; On same plane above the workspace by few even several cold light lamps of stationary arrangement at the most, the direction of the orientation of cold light lamp and refrigeration area and heating zone is rectangular, when light source all is in same plane, relevant with the density of intensity of illumination on another parallel plane of light source and light source corresponding points on this plane, density more big (being that measurement point and all light source distance sums are more little) intensity of illumination is more big, realize the graded of intensity of illumination thus by the control light source density, thereby in the metal grill of workspace, form continuous illumination gradient.Because thermograde and illumination gradient in metal grill are mutual vertical distribution, can in the metal grill of the workspace of casing, form the combination of a plurality of differing tempss and intensity of illumination, realize little algae light, warm complication experiment, realize little algae light, the disposable effect of finishing of warm complication experiment data.
The present invention is simple in structure, equipment cost is low, the mode of employing illumination, the two gradients controls of temperature quick, the high-throughout algae kind of carrying out is effectively screened and culture condition optimization, can reduce experiment labour intensity and experimental error in a large number, realize the little algae light of experiment, disposable the finishing of warm complication experiment data, the cripetura R﹠D cycle, simplify R﹠D process, characteristics with low, the easy making of cost, processing ease are for the research and development of algae provide an effective way.
Description of drawings
Fig. 1 is vertical section structure synoptic diagram of the present invention.
Fig. 2 is structural representation vertical view of the present invention.
Among the figure: 1, casing; 2, thermal insulation layer; 3, refrigeration area; 4, metal partion (metp); 5, workspace; 6, metal grill; 7, cold light lamp; 8, heating zone; 9, heat-conduction medium.
Embodiment
In the structure shown in the figure, the present invention includes casing 1, casing 1 is formed by the PMMA organic plates bonding of 5mm thickness, the outside adds metal supporting frames, the outer setting thermal insulation layer 2 of casing, in the left and right sides of casing refrigeration area 3 and heating zone 8 are set respectively, the middle part is workspace 5, heat transfer efficiencies preferred metal dividing plate 4 is set respectively on the relative position of refrigeration area and heating zone, refrigerator and thermostat are set in the refrigeration area, can freeze and keep the homo(io)thermism under the lower temperature condition (as maintaining 0 ℃) of refrigeration area by refrigerator and thermostat combination, heating rod and thermostat are set in the heating zone, can heat and keep the homo(io)thermism under the higher temperatures condition (as maintaining 45 ℃) of heating zone by heating rod and temperature controller combination; In the middle part of casing workspace 5 specification size same metal grid 6 is set evenly, in metal grill, is provided with heat-conduction medium 9, can carry out the heat conduction; On same plane above the workspace 5 by few even several cold light lamps 7 of stationary arrangement at the most, the direction of the orientation of cold light lamp 7 and refrigeration area and heating zone is (when the line that is refrigeration area and heating zone was horizontal, the orientation of cold light lamp was vertical) at right angles.Because the two ends in casing make a temperature end (heating zone 8) and a low-temperature end (refrigeration area 3), temperature head appears between the two ends, heat conducts naturally by the heat-conduction medium 9 in the middle of the two ends, changed continuously by high temperature (as 45 ℃) to low temperature (as 0 ℃), thereby utilize three kinds of natural ways (being thermal conduction, heat passage and thermal radiation) of transfer of heat in metal grill 6, to form continuous thermograde.Because cold light lamp 7 all is in same plane, relevant with the density of corresponding points on the intensity of illumination of metal grill 6 on another parallel plane of cold light lamp 7 and the cold light lamp 7, the more big intensity of illumination of density is more big, can realize the graded of intensity of illumination thus by the density of control cold light lamp 7, thereby in the metal grill 6 of workspace 5, form continuous illumination gradient.Because thermograde and illumination gradient in metal grill are mutual vertical distribution, can be effectively in the metal grill 6 of the workspace 5 of casing 1, form the combination of a plurality of differing tempss and intensity of illumination, realize little algae light, warm complication experiment, realize little algae light, the disposable effect of finishing of warm complication experiment data.
Described metal partion (metp) 4 is aluminium dividing plates.
Described metal grill 6 is aluminium grids.
Described heat-conduction medium 9 is heat of liquid transmission medium or solid thermal transmission medium.
Described heat of liquid transmission medium is water.
The present invention can be used for photosynthetic hydrobiont (little algae and kelp) and non-photosynthetic hydrobiological germ plasm resource screening and culture condition optimization.Can regulate temperature and the illumination range of incubator by the temperature of regulating light source density and cold and hot end, to adapt to the needs of different hydrobiont growths.Utilize different heat-conduction medium 9, can carry out different germ plasm resource screenings and culture condition optimization, during as employing solid thermal transmission medium, the present invention can carry out solid culture to little algae and microorganism.
Claims (4)
1. little algae incubator of light/temperature dual-gradient, it is characterized in that: comprise casing (1), the outer setting thermal insulation layer (2) of casing, refrigeration area (3) and heating zone (8) are set respectively in the left and right sides of casing, the middle part is workspace (5), metal partion (metp) (4) is set respectively on the relative position of refrigeration area and heating zone, refrigerator and thermostat are set in the refrigeration area, heating rod and thermostat are set in the heating zone; In the middle part of casing workspace (5) specification size same metal grid (6) is set evenly, in metal grill, is provided with the heat of liquid transmission medium; By few even several cold light lamps of stationary arrangement (7) at the most, the orientation of cold light lamp (7) and the direction of refrigeration area and heating zone are rectangular on the same plane, top of workspace (5).
2. the little algae incubator of light/temperature dual-gradient according to claim 1, it is characterized in that: described metal partion (metp) (4) is the aluminium dividing plate.
3. the little algae incubator of light/temperature dual-gradient according to claim 1, it is characterized in that: described metal grill (6) is the aluminium grid.
4. the little algae incubator of light/temperature dual-gradient according to claim 1, it is characterized in that: described heat of liquid transmission medium is water.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN 201110294544 CN102337204B (en) | 2011-10-02 | 2011-10-02 | Light/temperature dual-gradient microalgae incubator |
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| CN 201110294544 CN102337204B (en) | 2011-10-02 | 2011-10-02 | Light/temperature dual-gradient microalgae incubator |
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| CN102337204A CN102337204A (en) | 2012-02-01 |
| CN102337204B true CN102337204B (en) | 2013-07-03 |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105861302B (en) * | 2016-04-08 | 2018-02-13 | 东南大学 | The uniform illumination device of algae culture based on aquatile ecotoxicity assessment |
| CN107067927B (en) * | 2017-05-12 | 2024-04-05 | 中国林业科学研究院生态保护与修复研究所 | Algae horizontal migration simulation experiment device and simulation method |
| CN110964622A (en) * | 2019-12-20 | 2020-04-07 | 中国农业大学 | Gradient low-temperature biological enrichment reactor |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101760430A (en) * | 2009-04-24 | 2010-06-30 | 中国科学院海洋研究所 | Light and temperature dual-gradient culture precise data acquisition device |
| CN201751417U (en) * | 2010-07-01 | 2011-02-23 | 山东省科学院海洋仪器仪表研究所 | Tester simulating ocean environment |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101760430A (en) * | 2009-04-24 | 2010-06-30 | 中国科学院海洋研究所 | Light and temperature dual-gradient culture precise data acquisition device |
| CN201751417U (en) * | 2010-07-01 | 2011-02-23 | 山东省科学院海洋仪器仪表研究所 | Tester simulating ocean environment |
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| CN102337204A (en) | 2012-02-01 |
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