CN210048747U - Excrement coliform group's incubator - Google Patents
Excrement coliform group's incubator Download PDFInfo
- Publication number
- CN210048747U CN210048747U CN201920699090.6U CN201920699090U CN210048747U CN 210048747 U CN210048747 U CN 210048747U CN 201920699090 U CN201920699090 U CN 201920699090U CN 210048747 U CN210048747 U CN 210048747U
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- camera
- incubator
- fecal coliform
- transverse plate
- coliform group
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- 230000002550 fecal effect Effects 0.000 claims abstract description 38
- 238000005286 illumination Methods 0.000 claims abstract description 8
- 241000233866 Fungi Species 0.000 claims description 7
- 230000001678 irradiating effect Effects 0.000 claims description 2
- 238000012360 testing method Methods 0.000 abstract description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 abstract 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 16
- 238000000034 method Methods 0.000 description 5
- 238000006243 chemical reaction Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 208000011140 intestinal infectious disease Diseases 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 239000010865 sewage Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model provides a excrement coliform group mushroom incubator, include: the box body is provided with a through hole; the culture frame is arranged in the inner cavity of the box body and used for placing a quantitative disc, and the quantitative disc is used for placing fecal coliform; the camera is arranged on the culture frame and is used for shooting fecal coliform in the quantitative disc; the output line of the camera penetrates through the through hole and then is connected with a computer; the light source is arranged on the culture frame and used for providing illumination for the camera; and a power line of the light source penetrates through the through hole and then is connected with a power supply. Above-mentioned light source is used for providing the illumination for the camera in the scheme, and the staff of can being convenient for of setting up of camera observes excrement coliform crowd to improve the accuracy of test result.
Description
Technical Field
The utility model relates to a sewage treatment technical field, concretely relates to excrement coliform group incubator.
Background
Fecal coliform bacteria are one of the important indicators of water quality pollution, and in case of source water such as lake water, pond water and stream water, once polluted by fecal coliform bacteria, intestinal infectious diseases and even epidemic diseases can be caused. Therefore, the accurate, rapid and reliable fecal coliform group detection method has important significance for accurately and timely monitoring the pollution condition of the water source water fecal coliform group to effectively forecast and control the occurrence and spread of epidemic diseases.
In the process of detecting faecal coliform, the incubator is a necessary experimental instrument, the culture process of the incubator is a method for increasing the culture temperature, so that conditions which are not beneficial to the growth of the coliform from the natural environment are caused, the cultured bacteria are mainly the coliform from the excrement, and the condition that the water quality is polluted by the excrement is reflected more accurately.
The incubator in the prior art can provide a constant temperature environment to be different from an external environment, but an experimenter cannot observe constantly through the incubator in the culture process, so that the experiment conditions cannot be adjusted along with the progress of an experiment, and finally, the experiment result is uncertain.
Disclosure of Invention
The utility model discloses can't observe constantly when aiming at solving the incubator through among the prior art and cultivateing excrement coliform, lead to the test result to produce uncertain problem.
In order to solve the above problem, the utility model provides a excrement coliform group incubator, include:
the box body is provided with a through hole;
the culture frame is arranged in the inner cavity of the box body and used for placing a quantitative disc, and the quantitative disc is used for placing fecal coliform;
the camera is arranged on the culture frame and is used for shooting fecal coliform in the quantitative disc; the output line of the camera penetrates through the through hole and then is connected with a computer;
the light source is arranged on the culture frame and used for providing illumination for the camera; and a power line of the light source penetrates through the through hole and then is connected with a power supply.
Optionally, in the fecal coliform group incubator, the culture shelf includes:
the first fixing frame and the second fixing frame are oppositely arranged;
the transverse plates are arranged in parallel, one end of each transverse plate is connected with the first fixing frame, and the other end of each transverse plate is connected with the second fixing frame;
the quantitative discs are placed on the upper surfaces of the other transverse plates except the topmost transverse plate; the lower surfaces of the other transverse plates except the bottommost transverse plate fix the camera and the light source.
Optionally, in the fecal coliform group incubator:
the light source is an LED illuminating lamp.
Optionally, the fecal coliform group incubator further comprises:
the ultraviolet lamps are arranged on the lower surfaces of the transverse plates except the transverse plate at the bottommost end and are used for irradiating fecal coliform groups in the quantitative plate; and a power line of the ultraviolet lamp penetrates through the through hole and then is connected with a power supply.
Optionally, in the fecal coliform group incubator:
be fixed with two on the lower surface of diaphragm the LED light and two the ultraviolet lamp, the camera is located the middle part of the lower surface of diaphragm, two the LED light is located the both sides of camera, two the ultraviolet lamp is located the both sides of camera.
Optionally, in the fecal coliform group incubator:
at least one groove is formed in the upper surface of the transverse plate and used for fixing the quantitative disc.
Optionally, in the fecal coliform group incubator, the first fixing frame and the second fixing frame each include:
the first bracing piece and the second bracing piece of vertical setting to and many connecting rods of level setting, each the one end of connecting rod with first bracing piece is connected, each the other end of connecting rod with the second bracing piece is connected, the one end of diaphragm with the connecting rod is connected.
Optionally, in the fecal coliform group incubator:
the first support rod and the second support rod of the first fixing frame are both provided with first rollers, the first rollers and the transverse plate are positioned on the same side of the first fixing frame, and the upper surface of each first roller is flush with the upper surface of the transverse plate at the corresponding position of the first roller;
all be provided with the second gyro wheel on the first bracing piece of second mount and the second bracing piece, the second gyro wheel with the diaphragm is located the homonymy of second mount, just the upper surface of second gyro wheel is rather than corresponding position department the upper surface parallel and level of diaphragm.
Optionally, the fecal coliform group incubator further comprises:
and the supporting net rack is horizontally arranged above the first idler wheel, the second idler wheel and the transverse plate.
Optionally, the fecal coliform group incubator further comprises:
the temperature sensor is arranged in the inner cavity of the box body and used for detecting a temperature value in the inner cavity of the box body, and the output end of the temperature sensor outputs a temperature signal representing the temperature value;
the display screen is arranged outside the box body, and the input end of the display screen is connected with the output end of the temperature sensor; and the display screen displays the temperature value represented by the temperature signal after receiving the temperature signal.
Excrement coliform crowd incubator, including box, cultivation frame, camera and light source, when placing the ration dish that contains the excrement and rise the fungus crowd in the inner chamber of box greatly, box body and chamber door that the box includes can play the effect that keeps warm and keep apart, the camera can be arranged in shooting the change that the excrement rises the fungus crowd greatly in the ration dish, the light source can be used to for the camera illumination, the video in the staff's accessible was looked over the computer and is observed the excrement and rise the fungus crowd to adjust the experimental condition in real time, and then improve the accuracy of experimental result.
Drawings
FIG. 1 is a schematic structural view of a fecal coliform group incubator according to an embodiment of the present invention;
FIG. 2 is a schematic view of the internal structure of a fecal coliform group incubator according to an embodiment of the present invention;
FIG. 3 is a schematic structural view of a culture support of a fecal coliform group incubator according to an embodiment of the present invention;
fig. 4 is a schematic view of an installation structure of an LED lighting lamp, an ultraviolet lamp and a camera of a fecal coliform group incubator according to an embodiment of the present invention;
fig. 5 is a schematic structural view of a supporting net frame of a fecal coliform group incubator according to an embodiment of the present invention.
Detailed Description
The present invention will be described in further detail by way of examples with reference to the accompanying drawings, which are illustrative of the present invention and are not intended to limit the present invention.
Referring to fig. 1 and 2, the embodiment provides a fecal coliform group incubator, which comprises a incubator body, wherein the incubator body comprises an incubator body 1 and an incubator door 3, the incubator door 3 is hinged to the incubator body 1, and when the incubator door 3 is closed, the incubator body is in a closed state, and an inner cavity 2 is formed in the incubator body. A culture frame 4 for placing a quantitative disc 13 is arranged in the inner cavity 2 of the box body, and the quantitative disc 13 is used for placing fecal coliform groups. The culture shelf 4 is provided with a camera 12 and a light source 10, the camera 12 is used for shooting faecal coliform in the quantitative disc 13, and the light source 10 is used for providing illumination for the camera 12. The box body is provided with a through hole, an output line of the camera 12 penetrates through the through hole in the box body and then is connected with a computer, and a power line of the light source penetrates through the through hole and then is connected with a power supply.
This embodiment the excrement coliform crowd incubator when placing the ration dish 13 that contains the excrement and rise the fungus crowd in the inner chamber 2 of box, box body 1 and chamber door 3 play the effect of heat preservation isolation, camera 12 can be arranged in shooting the change of the excrement and rise the fungus crowd in the ration dish 13, light source 10 can be used to for the illumination of camera 12, the video of staff's accessible in looking over the computer observes the excrement and rises the fungus crowd to adjust the experimental condition in real time, and then improve the accuracy of experimental result.
As shown in fig. 3, the cultivation shelf 4 includes a first fixing frame 8, a second fixing frame 9 and a plurality of transverse plates 7, which are oppositely arranged, and the plurality of transverse plates 7 are sequentially arranged in parallel from top to bottom. In fig. 3, 5 transverse plates 7 are shown, and 5 transverse plates 7 are arranged in parallel from top to bottom. One end of each transverse plate 7 is connected with the first fixing frame 8, and the other end of each transverse plate 7 is connected with the second fixing frame 9. The quantitative disc 13 is arranged on the upper surface of the transverse plate 7, and the camera 12 and the light source 10 are arranged on the lower surface of the transverse plate 7 above the quantitative disc 13. That is, as shown in fig. 3, except for the lowest horizontal plate 7, the lower surfaces of the other horizontal plates 7 are fixed with the light source 10 and the camera 12, except for the highest horizontal plate 7, the upper surfaces of the other horizontal plates 7 are used for placing the quantitative trays 13, so that one camera 12 can be used for shooting all the quantitative trays 13 on one horizontal plate 7. The light source can be an LED illuminating lamp or other devices capable of illuminating. In addition, above-mentioned scheme still includes ultraviolet lamp 11, ultraviolet lamp 11 is used for shining quantitative dish 13 to make the excrement coliform group in the quantitative dish 13 produce the fluorescence reaction, in order to judge whether there is excrement coliform group, just ultraviolet lamp 11 sets up in the lower surface of other diaphragm 7 except bottommost diaphragm 7. Specifically, as shown in fig. 4, be fixed with two light sources 10 and two ultraviolet lamps 11 on the lower surface of diaphragm 7, camera 12 is located the middle part of the lower surface of diaphragm 7, two light sources 10 are located camera 12's both sides, two ultraviolet lamps 11 are located camera 12's both sides, two light sources 10 can provide abundant illumination for camera 12, and two ultraviolet lamps 11 can make excrement coliform group produce abundant fluorescence reaction. The power cords of the light source 10 and the ultraviolet lamp 11 penetrate through the through hole of the box body and then are connected with the power source, the camera 12 is connected to the data line, the data line penetrates out of the through hole of the box body, the end portion of the data line is a USB connector, and the USB connector is suitable for being connected with a computer.
As shown in fig. 3, except the uppermost transverse plate 7, the upper surfaces of the other transverse plates 7 are provided with at least one groove 14 for placing the quantitative plate 13, and when the grooves 14 are multiple, the grooves 14 on each transverse plate 7 are sequentially arranged from left to right. The size and shape of the cross section of the groove 14 are the same as those of the cross section of the dosing disc 13, so that when the dosing disc 13 is placed in the groove 14, the groove 14 can restrict the movement of the dosing disc 13 and prevent the dosing disc 13 from falling.
As shown in fig. 3, the first fixing frame 8 and the second fixing frame 9 are respectively composed of a first support rod 15, a second support rod 16 and a plurality of horizontally arranged connecting rods 17, each connecting rod 17 has one end connected to the first support rod 15, the other end connected to the second support rod 16, and the plurality of connecting rods 17 are sequentially arranged from top to bottom. The arrangement shown in figure 3 comprises 5 links 17, the 5 links 17 being spaced one above the other. When the transverse plate 7 is connected, one end of the transverse plate 7 is connected with the connecting rod 17 of the first fixing frame 8, the other end of the transverse plate 7 is connected with the connecting rod 17 of the second fixing frame 9, and the transverse plate 7, one connecting rod 17 of the first fixing frame 8 and one connecting rod 17 of the second fixing frame 9 are located in the same horizontal plane.
As shown in fig. 3, the first support rod 15 and the second support rod 16 of the first fixing frame 8 of the culture support 4 are both provided with a first roller 5, the first roller 5 and the transverse plate 7 are located at the same side of the first fixing frame 8, and the upper surface of the first roller 5 is flush with the upper surface of the transverse plate 7 at the corresponding position, that is, the upper surface of one first roller 5 is flush with the upper surface of one connecting rod 17 of the first fixing frame 8. The first support rod 15 and the second support rod 16 of the second fixing frame 9 are both provided with a second roller 18, the second roller 18 and the transverse plate 7 are located on the same side of the second fixing frame 9, the upper surface of the second roller 18 is flush with the upper surface of the transverse plate 7 at the corresponding position, that is, the upper surface of one second roller 18 is flush with the upper surface of one connecting rod 17 of the second fixing frame 9. By arranging the first roller 5 and the second roller 18, the quantitative trays 13 can be placed in the grooves 14, and the supporting net rack 6 can be supported on the first roller 5 and the second roller 18 and the transverse plate 7 by pushing in the supporting net rack 6 shown in fig. 5, so that the placing requirements of a large number of quantitative trays 13 are met.
In addition, still be provided with temperature sensor in the inner chamber 2 of box, temperature sensor is used for detecting temperature value in the inner chamber 2, temperature sensor's output representation the temperature signal of temperature value. The temperature control box comprises a box body, and is characterized in that a display screen is arranged outside the box body, the input end of the display screen is connected with the output end of a temperature sensor, and the display screen receives a temperature signal and then displays a temperature value represented by the temperature signal.
The method for monitoring and detecting the water quality of the fecal coliform box body comprises the following steps: the water sample required for detection is 100 mL.
(1) 100mL of water sample is measured by a 100mL sterile dilution bottle, MUG culture medium powder is added, the mixture is uniformly mixed and shaken to be completely dissolved, the water sample is completely poured into a 5L sterile quantitative disc 13, the back of the quantitative disc 13 is smoothed by hands to remove bubbles in holes, and then a sealing machine is used for sealing.
(2) After being put into a box body for culturing for 24 hours, the water sample is illuminated by a light source 10, and is observed by a quantitative disc 13 in cooperation with a camera 12 (when the water sample is used, a USB connector of a data line needs to be connected to a computer terminal), and if the water sample in the hole turns yellow, the water sample indicates that the hole contains coliform groups.
(3) Then, after the light source 10 was turned off, the measurement dish 13 was observed by the ultraviolet lamp 11 with a wavelength of 366nm in cooperation with the camera 12, and if there was a hole for a yellow reaction and blue fluorescence was generated, it was indicated that Escherichia coli was contained in the hole of the measurement dish 13.
(4) The number of holes with yellow reaction and fluorescence is calculated, and the most probable number of faecal coliform represented by the holes is checked out from an MPN table.
The above description in this specification is merely illustrative of the present invention. Various modifications, additions and substitutions may be made by those skilled in the art without departing from the scope of the invention as defined in the accompanying claims.
Claims (10)
1. The utility model provides a excrement coliform group fungus incubator which characterized in that includes:
the box body is provided with a through hole;
the culture frame is arranged in the inner cavity of the box body and used for placing a quantitative disc, and the quantitative disc is used for placing fecal coliform;
the camera is arranged on the culture frame and is used for shooting fecal coliform in the quantitative disc; the output line of the camera penetrates through the through hole and then is connected with a computer;
the light source is arranged on the culture frame and used for providing illumination for the camera; and a power line of the light source penetrates through the through hole and then is connected with a power supply.
2. The fecal coliform group incubator of claim 1, wherein the incubator comprises:
the first fixing frame and the second fixing frame are oppositely arranged;
the transverse plates are arranged in parallel, one end of each transverse plate is connected with the first fixing frame, and the other end of each transverse plate is connected with the second fixing frame;
the quantitative discs are placed on the upper surfaces of the other transverse plates except the topmost transverse plate; the lower surfaces of the other transverse plates except the bottommost transverse plate fix the camera and the light source.
3. The fecal coliform group incubator of claim 2, wherein:
the light source is an LED illuminating lamp.
4. The fecal coliform group incubator of claim 3, further comprising:
the ultraviolet lamps are arranged on the lower surfaces of the transverse plates except the transverse plate at the bottommost end and are used for irradiating fecal coliform groups in the quantitative plate; and a power line of the ultraviolet lamp penetrates through the through hole and then is connected with a power supply.
5. The fecal coliform group incubator of claim 4, wherein:
be fixed with two on the lower surface of diaphragm the LED light and two the ultraviolet lamp, the camera is located the middle part of the lower surface of diaphragm, two the LED light is located the both sides of camera, two the ultraviolet lamp is located the both sides of camera.
6. The fecal coliform group incubator according to any one of claims 2-5, wherein:
at least one groove is formed in the upper surface of the transverse plate and used for fixing the quantitative disc.
7. The fecal coliform group incubator of any of claims 2-5, wherein the first mount and the second mount each comprise:
the first bracing piece and the second bracing piece of vertical setting to and many connecting rods of level setting, each the one end of connecting rod with first bracing piece is connected, each the other end of connecting rod with the second bracing piece is connected, the one end of diaphragm with the connecting rod is connected.
8. The fecal coliform group incubator of claim 7, wherein:
the first support rod and the second support rod of the first fixing frame are both provided with first rollers, the first rollers and the transverse plate are positioned on the same side of the first fixing frame, and the upper surface of each first roller is flush with the upper surface of the transverse plate at the corresponding position of the first roller;
all be provided with the second gyro wheel on the first bracing piece of second mount and the second bracing piece, the second gyro wheel with the diaphragm is located the homonymy of second mount, just the upper surface of second gyro wheel is rather than corresponding position department the upper surface parallel and level of diaphragm.
9. The fecal coliform group incubator of claim 8, further comprising:
and the supporting net rack is horizontally arranged above the first idler wheel, the second idler wheel and the transverse plate.
10. The fecal coliform group incubator of claim 9, further comprising:
the temperature sensor is arranged in the inner cavity of the box body and used for detecting a temperature value in the inner cavity of the box body, and the output end of the temperature sensor outputs a temperature signal representing the temperature value;
the display screen is arranged outside the box body, and the input end of the display screen is connected with the output end of the temperature sensor; and the display screen displays the temperature value represented by the temperature signal after receiving the temperature signal.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920699090.6U CN210048747U (en) | 2019-05-16 | 2019-05-16 | Excrement coliform group's incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920699090.6U CN210048747U (en) | 2019-05-16 | 2019-05-16 | Excrement coliform group's incubator |
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| CN210048747U true CN210048747U (en) | 2020-02-11 |
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| CN201920699090.6U Active CN210048747U (en) | 2019-05-16 | 2019-05-16 | Excrement coliform group's incubator |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111693443A (en) * | 2020-06-11 | 2020-09-22 | 浙江泰林生命科学有限公司 | Automatic coliform group counting and tracing system |
| CN113667592A (en) * | 2021-07-23 | 2021-11-19 | 江苏省环境监测中心 | Online rapid detection system and method for field fecal coliform |
-
2019
- 2019-05-16 CN CN201920699090.6U patent/CN210048747U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111693443A (en) * | 2020-06-11 | 2020-09-22 | 浙江泰林生命科学有限公司 | Automatic coliform group counting and tracing system |
| CN113667592A (en) * | 2021-07-23 | 2021-11-19 | 江苏省环境监测中心 | Online rapid detection system and method for field fecal coliform |
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