CN105853341A - Anti-aging skincare product containing epidermal stem cell secretin and preparation method of anti-aging skincare product - Google Patents
Anti-aging skincare product containing epidermal stem cell secretin and preparation method of anti-aging skincare product Download PDFInfo
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- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
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Abstract
The invention belongs to the field of skincare products, in particular to an anti-aging skincare product containing epidermal stem cell secretin and a preparation method of the anti-aging skincare product. The skincare product is prepared from the following components by weight percentage: 0.000001-0.0005% of epidermal stem cell secretin, 2-8% of plant extract, 1-2% of squalane, 0.10-0.25% of EDTA-2Na, 5-10% of glycerol, 0.05-1% of poly-glutamic acid, 0.06-0.12% of K350, 2-6% of isopropyl myristate, 0.5-2% of cetearyl alcohol, 1-2% of polydimethylsiloxane and the balance of water, wherein the plant extract is prepared from semen adenantherae pavoninae, edgeworthia chrysantha, radix acanthopanacis senticosi, ilicis rotundae cortex and fructus rubi. The skincare product provided by the invention can significantly improve skin aging, delay aging, reduce the incidence of adverse reactions, is simple in preparation method and stable in property.
Description
Technical field
The invention belongs to skin care field, be specifically related to a kind of anti-aging skin care product containing epidermal stem cells secretin and preparation thereof
Method.
Background technology
Skin senescence is the skin senescence phenomenon caused by natural cause or non-natural factor, shows as the appearance of wrinkle, skin
Lax, scrambling pigmentation, blood vessel dilatation, keratinization of epidermis is bad, abnormality proliferation occur, corium elastomer sex change and
Catabolite accumulation etc..Skin senescence is closely related with cell ageing, especially with fibroblast and the aging of keratinocyte
It is closely connected.The secretion of fibroblast collagen during aging reduces, and the inducibility of cell glue protoenzyme raises simultaneously,
Causing collagen to decompose to strengthen, thus promote skin elasticity to decline, wrinkle occurs.Additionally, keratinocyte is protected at skin
Water, moisturizing, barrier and the many-side such as skin formation have very important regulation effect.The mistake that keratinocyte is old and feeble
Gene expression relevant to skin repair in journey is slow, and damages relevant gene expression and increase, thus promotes skin aging.
Stem cell is the seed cell of a kind of not yet differentiation and development, has unlimited self replication capacity, and can be divided into specific
Tissue, this is that stem cell is for improving looks or even the basis of regeneration.Research shows, in old and feeble animal body, and epidermis
Cell metabolization rate slows down, and therefore skin speckle increases, and wrinkle increases.The major function of epidermal stem cells is supplementary table chrotoplast
Maintain the balance of Skin Cell and repair damaged tissues.The Proliferation, Differentiation promoting epidermal stem cells just can make skin keep vigorous
Metabolism.Additionally, the self-replacation of stem cell and pluripotent differentiation also rely on the collaborative facilitation of multiple growth factor,
Research shows, stem cell can secrete some active materials in incubation, including growth factor, cell factor, active peptides,
The materials such as protein, growth hormone, trace element, promote the amplification of self and the propagation of the daughter cell of differentiation.
At present, existing people promotes cell-secretion factor (plain also known as stem cell secretion) from the raffinate that stem cell is cultivated and is used for making up
Product.Tai lifts " the first cellularised cosmetic of branch in the whole world will list Korea Spro " delivered at Science and Technology Daily and the article pointed out Korea S in 2011
FCB Pharmicell company have developed human stem cell nutrient solution cosmetics with stem cell nutrient solution for core material, should
Company in experiments it is found that the collagen amount generated in the SF injecting stem cell nutrient solution is control group
More than 5 times.Chinese patent application 201110452259.6 disclose a kind of composite biological agent used for skin beautifying and restoring and
Preparation method, this composite biological agent contains cord blood stem cell active component and placenta active component, has promotion puerpera's epidermis
The metabolism of stem cell, thus realize beauty treatment and repair, the purpose of plaque dispelling skin care, but described composite biological agent is according to conventional
Skin care item production method is prepared from, will cord blood stem cell active matter, placenta active matter in proportion with conventional cosmetic
Inert matter mixing makes frost class cosmetics etc. according to conventional skin care item production method.It is known that in stem cell cosmetics
Active component is easy to inactivate at normal temperatures, will keep its activity, has been necessary under specified conditions preparing and preserving.Therefore,
The effect of these type of stem cell cosmetics remains to be discussed.Additionally, the stem cell extract of heterologous is applied to people easily causes allergy
The bad reactions such as reaction.
Summary of the invention
It is an object of the invention to provide a kind of can effectively skin be repaired and will not cause allergic reaction containing epidermis
Anti-aging skin care product of stem cell secretion element and preparation method thereof.Described anti-aging skin care product active component can preferably preserve,
Thus give full play to it and promote skin hyperplasia ability, improve skin elasticity, improve the effect of skin quality, there is not the reasons such as heterologous
The phenomenons such as the allergic reaction caused, and preparation method is simple, stable in properties.
The present invention is by following technical solution to be attained in that
A kind of anti-aging skin care product containing epidermal stem cells secretin, it is characterised in that described skin care item are by following weight percent
Component composition than meter: epidermal stem cells secretin 0.000001-0.0005%, plant extracts 2～8%, saualane 1～2%,
EDTA-2Na 0.10～0.25%, glycerine 5～10%, polyglutamic acid 0.05～1%,K350 0.06～0.12%, Pork and beans
Cool isopropyl propionate 2～6%, cetostearyl alcohol 0.5～2%, dimethyl silicone polymer 1～2%, surplus is water.
Preferably, described anti-aging skin care product, it is characterised in that described skin care item are by the component group of following percentage by weight meter
Become: epidermal stem cells secretin 0.000125%, plant extracts 6%, saualane 1%, EDTA-2Na 0.15%, glycerine
8%, polyglutamic acid 0.50%,K350 0.10%, isopropyl myristate 4%, cetostearyl alcohol 1%, poly-diformazan
Radical siloxane 2%, surplus is water.
Further, the mixture of the various active factor of the epidermal stem cells secretion that described epidermal stem cells secretin is behaved.
Further, described plant extracts is prepared by the raw material of following weight percentage: circassian bean 16～28%, avenge beautiful
Spend 14～30%, wilsonii 12～28%, purpleflower holly bark 14～26% and raspberry 10～22%.
Preferably, described plant extracts is prepared by the raw material of following weight percentage: circassian bean 22%, sherry flower 22%,
Wilsonii 20%, purpleflower holly bark 20% and raspberry 16%.
Further, the preparation method of described epidermal stem cells secretin comprises the following steps:
(1) acquisition of human epidermal stem cell
Take surgery excision or scraping oral epithelium tissue, tissue is proceeded to containing penicillin, streptomysin and gentamicin sulphate
In D-PBSA (phosphate buffer), under the conditions of dry ice or liquid nitrogen frozen, transport laboratory back.Rinsing 2-3 time repeatedly, adds
37 DEG C of trypsase digestion 30min, shreds oral epithelium tissue, filters epithelial cell with 70-100um cell filter,
Collect filtrate.Under the conditions of 1200r/min, centrifugal 5min, abandons supernatant, soft with Defined Keratinocyte-SFM culture medium
Piping and druming, obtains cell suspension, utilizes the collagen isolated and purified human epidermal stem cell of rapid attachment method；
(2) cultivation of human epidermal stem cell
The human epidermal stem cell that step (1) obtains is seeded in containing 100U/ml penicillin and 100ug/ml streptomysin
In Defined Keratinocyte-SFM culture medium, 37 DEG C, 5%CO2Incubator hatches 12-24h, abandons culture medium, again adds
Enter fresh containing penicillin and streptomysin Defined Keratinocyte-SFM culture medium, cultivate 1-3d, abandon culture medium, add
Enter Defined Keratinocyte-SFM culture medium to continue to cultivate.When human epidermal stem cell merges close to 90%, Secondary Culture；
(3) preparation of epidermal stem cells secretin
Human epidermal stem cell is expanded 1-5 generation, collects culture supernatant, the supernatant sterilizing filter of 0.22 μm is filtered,
The filtrate merging every generation i.e. obtains epidermal stem cells secretin.
Further, the preparation method of described plant extracts comprises the following steps:
Take ingredients the most in proportion, wash away impurity, crushed after being dried, cross 50～60 mesh sieves, merge meal, add medicinal material
Meal gross weight 8～12 times amount volume fraction is the ethanol of 50～70%, and Microwave Extraction 4～6 minutes, microwave power is
400～500W, Extracting temperature is 40～60 DEG C, is filtered by extract, repeats to extract 1 time by similarity condition, merges extract,
It is evaporated at 60 DEG C survey the medicinal extract that relative density is 1.05～1.15, prepares plant extracts.
Additionally, the present invention also provides for the preparation method of described anti-aging skin care product, it comprises the following steps:
Step 1: epidermal stem cells secretin, excipient, water for injection are mixed, carries out vacuum freeze drying, must freeze
Dry powder is standby；
Step 2: EDTA-2Na, polyglutamic acid and water are mixed, be heated to 80 DEG C, homogeneous 6～10min, stirring is to dissolving
Completely, material A is obtained；
Step 3: glycerine, saualane, isopropyl myristate, cetostearyl alcohol and dimethyl silicone polymer are mixed, heating
To 80～90 DEG C, homogeneous 10～15min, stirring, to dissolving completely, obtains material B；Material B it is slowly added in material A,
Stir, start cooling；
Step 4: when temperature is down to about 30 DEG C, to emulsification pot in add plant extracts,K350 and step 1
The freeze-dried powder prepared, stirs, to plate discharging；
Wherein, the excipient described in step 1 is the mixture that mannitol and L-arginine prepare with 1: 1 proportioning；
Wherein, the vacuum freeze drying step of step 1 is: by epidermal stem cells secretin, excipient, water for injection mixing all
Even it is placed in-70 DEG C of freeze dryers, is cooled to less than-50 DEG C, pre-freeze 6h, vacuumize 8h, be to slowly warm up to-20 DEG C, then with
The speed of 5 DEG C/h is warming up to 25 DEG C, and heat preservation and dryness 2h to obtain final product.
Compared with prior art, present invention have an advantage that
(1) active component of the anti-aging skin care product that the present invention provides is epidermal stem cells secretin and plant extracts, described
The mixture of the various active factor of epidermal stem cells secretion behaved of epidermal stem cells secretin, stem cell therefore can be avoided to carry
Take the immunological rejection that thing heterologous causes, reduce anaphylactoid incidence；Additionally, the active ingredient in plant extracts
There is promotion Transdermal absorption effect, make the active component of anti-aging skin care product be deep into dermal layer of the skin and hypodermis gives full play to
Repair skin, promote effect of skin hyperplasia ability；Press down it addition, the active ingredient in plant extracts also has proteolytic enzyme
Make use, thus delay the bioactive ingredients in epidermal stem cells secretin by albumen in dermal cell and subcutaneous tissue cell
The speed of hydrolase, improves epidermal stem cells secretin senile-resistant efficacy.
(2) present invention is during preparing anti-aging skin care product, uses Vacuum Freezing & Drying Technology that epidermal stem cells is secreted
Element makes freeze-dried powder, is conducive to preserving the bioactive ingredients in epidermal stem cells secretin, thus preferably plays anti-aging merit
Effect.
(3) anti-aging skin care product that the present invention provides can significantly improve skin aging, reduces the generation of bad reaction, increases angle
Matter layer water content and fat content, its effect is better than Shiseido and swings Lay many vigor emulsion, and preparation method is simple, stable in properties,
It is suitable for industrialized production.
Detailed description of the invention
Further describe the present invention below by way of detailed description of the invention, but the present invention is not limited only to following example.
Embodiment 1
Anti-aging skin care product described in the embodiment of the present invention 1 is made up of the component of following percentage by weight meter: epidermal stem cells is secreted
Element 0.000125%, plant extracts 6%, saualane 1%, EDTA-2Na 0.15%, glycerine 8%, polyglutamic acid 0.50%,K350 0.10%, isopropyl myristate 4%, cetostearyl alcohol 1%, dimethyl silicone polymer 2%, surplus is water；
The mixture of the various active factor of the epidermal stem cells secretion that described epidermal stem cells secretin is behaved；Described plant extracts by
The raw material of following weight percentage is prepared: circassian bean 22%, sherry flower 22%, wilsonii 20%, purpleflower holly bark 20% and
Raspberry 16%.
Preparation method:
(1) preparation of epidermal stem cells secretin:
The acquisition of human epidermal stem cell: take surgery excision or scraping oral epithelium tissue, tissue is proceeded to containing penicillin, strepto-
In the D-PBSA (phosphate buffer) of element and gentamicin sulphate, under the conditions of dry ice or liquid nitrogen frozen, transport laboratory back.Instead
Rinsing 3 times again, add 37 DEG C of trypsase digestion 30min, are shredded by oral epithelium tissue, use 100um cell filter
Filter epithelial cell, collect filtrate.Under the conditions of 1200r/min, centrifugal 5min, abandons supernatant, uses Defined Keratinocyte-SFM
Culture medium is softly blown and beaten, and obtains cell suspension, utilizes the collagen isolated and purified human epidermal stem cell of rapid attachment method；
The cultivation of human epidermal stem cell: human epidermal stem cell is seeded in containing 100U/ml penicillin and 100ug/ml streptomysin
In Defined Keratinocyte-SFM culture medium, 37 DEG C, 5%CO2Incubator hatches 24h, abandons culture medium, rejoins
Fresh containing penicillin and streptomysin Defined Keratinocyte-SFM culture medium, cultivate 2d, abandon culture medium, add
Defined Keratinocyte-SFM culture medium continues to cultivate.When human epidermal stem cell merges close to 90%, Secondary Culture；
The preparation of epidermal stem cells secretin: human epidermal stem cell expanded 5 generations, collects culture supernatant, is used by supernatant
The sterilizing filter of 0.22 μm filters, and the filtrate merging every generation i.e. obtains epidermal stem cells secretin；
(2) preparation of plant extracts:
Take ingredients the most in proportion, wash away impurity, crushed after being dried, cross 60 mesh sieves, merge meal, add medicinal material thick
Powder gross weight 10 times amount volume fraction is the ethanol of 60%, Microwave Extraction 5 minutes, and microwave power is 450W, Extracting temperature
It is 45 DEG C, extract is filtered, repeats to extract 1 time by similarity condition, merge extract, be evaporated at 60 DEG C survey phase
It is the medicinal extract of 1.05 to density, prepares plant extracts；
(3) preparation of anti-aging skin care product:
Step 1: epidermal stem cells secretin, excipient, water for injection are mixed and is placed in-70 DEG C of freeze dryers, cold
But to less than-50 DEG C, pre-freeze 6h, vacuumize 8h, be to slowly warm up to-20 DEG C, then be warming up to 25 DEG C with the speed of 5 DEG C/h,
Heat preservation and dryness 2h, obtains freeze-dried powder standby；
Step 2: EDTA-2Na, polyglutamic acid and water are mixed, be heated to 80 DEG C, homogeneous 6～10min, stirring is to dissolving
Completely, material A is obtained；
Step 3: glycerine, saualane, isopropyl myristate, cetostearyl alcohol and dimethyl silicone polymer are mixed, heating
To 80～90 DEG C, homogeneous 10～15min, stirring, to dissolving completely, obtains material B；Material B it is slowly added in material A,
Stir, start cooling；
Step 4: when temperature is down to about 30 DEG C, to emulsification pot in add plant extracts,K350 and step 1
The freeze-dried powder prepared, stirs, to plate discharging；
Wherein, the excipient described in step 1 is the mixture that mannitol and L-arginine prepare with 1: 1 proportioning.
Embodiment 2
Anti-aging skin care product described in the embodiment of the present invention 2 is made up of the component of following percentage by weight meter: epidermal stem cells is secreted
Element 0.0005%, plant extracts 8%, saualane 2%, EDTA-2Na 0.25%, glycerine 10%, polyglutamic acid 1%,
K350 0.12%, isopropyl myristate 6%, cetostearyl alcohol 2%, dimethyl silicone polymer 2%, surplus is water；Described
The mixture of the various active factor of the epidermal stem cells secretion that epidermal stem cells secretin is behaved；Described plant extracts is by following
The raw material of weight percentage is prepared: circassian bean 16%, sherry flower 14%, wilsonii 28%, purpleflower holly bark 20% and cover basin
Son 22%.
Preparation method is with embodiment 1.
Embodiment 3
Anti-aging skin care product described in the embodiment of the present invention 3 is made up of the component of following percentage by weight meter: epidermal stem cells is secreted
Element 0.000001%, plant extracts 2%, saualane 1%, EDTA-2Na 0.10%, glycerine 5%, polyglutamic acid 0.05%,K350 0.06%, isopropyl myristate 2%, cetostearyl alcohol 0.5%, dimethyl silicone polymer 1%, surplus is
Water；The mixture of the various active factor of the epidermal stem cells secretion that described epidermal stem cells secretin is behaved；Described plant extract
Thing is prepared by the raw material of following weight percentage: circassian bean 28%, sherry flower 30%, wilsonii 12%, purpleflower holly bark 20%
With raspberry 10%.
Preparation method is with embodiment 1.
Embodiment 4
Anti-aging skin care product described in the embodiment of the present invention 4 is made up of the component of following percentage by weight meter: epidermal stem cells is secreted
Element 0.00025%, plant extracts 6%, saualane 1%, EDTA-2Na 0.15%, glycerine 8%, polyglutamic acid 0.25%,K350 0.10%, isopropyl myristate 4%, cetostearyl alcohol 1%, dimethyl silicone polymer 2%, surplus is water；
The mixture of the various active factor of the epidermal stem cells secretion that described epidermal stem cells secretin is behaved；Described plant extracts by
The raw material of following weight percentage is prepared: circassian bean 20%, sherry flower 20%, wilsonii 20%, purpleflower holly bark 26% and
Raspberry 14%.
Preparation method is with embodiment 1.
Embodiment 5
Anti-aging skin care product described in the embodiment of the present invention 5 is made up of the component of following percentage by weight meter: epidermal stem cells is secreted
Element 0.00005%, plant extracts 8%, saualane 2%, EDTA-2Na 0.25%, glycerine 10%, polyglutamic acid 0.75%,K350 0.12%, isopropyl myristate 6%, cetostearyl alcohol 2%, dimethyl silicone polymer 2%, surplus is water；
The mixture of the various active factor of the epidermal stem cells secretion that described epidermal stem cells secretin is behaved；Described plant extracts by
The raw material of following weight percentage is prepared: circassian bean 28%, sherry flower 30%, wilsonii 12%, Chinese ilex 14% and cover
Basin 16%.
Preparation method is with embodiment 1.
Comparative example 1
Anti-aging skin care product described in comparative example 1 of the present invention is made up of the component of following percentage by weight meter: epidermal stem cells is secreted
Element 0.000125%, saualane 1%, EDTA-2Na 0.15%, glycerine 8%, polyglutamic acid 0.50%,K350 0.10%,
Isopropyl myristate 4%, cetostearyl alcohol 1%, dimethyl silicone polymer 2%, surplus is water；Described epidermal stem cells divides
The mixture of the various active factor of the epidermal stem cells secretion that secretin is behaved.(compared with Example 1 lack plant extracts this
One component)
Preparation method reference example 1.
Comparative example 2
Anti-aging skin care product described in comparative example 2 of the present invention is made up of the component of following percentage by weight meter: plant extracts 6%,
Saualane 1%, EDTA-2Na 0.15%, glycerine 8%, polyglutamic acid 0.50%,K350 0.10%, myristic acid
Isopropyl ester 4%, cetostearyl alcohol 1%, dimethyl silicone polymer 2%, surplus is water；Described plant extracts is by following weight
The raw material of percentage meter is prepared: circassian bean 22%, sherry flower 22%, wilsonii 20%, purpleflower holly bark 20% and raspberry 16%.
(lacking this component of epidermal stem cells secretin compared with Example 1)
Preparation method reference example 1.
Comparative example 3
Anti-aging skin care product described in comparative example 3 of the present invention is made up of the component of following percentage by weight meter: epidermal stem cells is secreted
Element 0.000125%, plant extracts 6%, saualane 1%, EDTA-2Na 0.15%, glycerine 8%, polyglutamic acid 0.50%,K350 0.10%, isopropyl myristate 4%, cetostearyl alcohol 1%, dimethyl silicone polymer 2%, surplus is water；
The mixture of the various active factor of the epidermal stem cells secretion that described epidermal stem cells secretin is behaved；Described plant extracts by
The raw material of following weight percentage is prepared: sherry flower 30%, wilsonii 28%, purpleflower holly bark 20% and raspberry 22%.
(plant extracts described compared with Example 1 lacks this component of circassian bean)
Preparation method reference example 1.
Test example one, anti-aging skin care product resisting age of skin clinical observation of the present invention
1. test method
Screen 540 example skins and the aging tested volunteer of women occur, age 28-50 year, 35 years old mean age, inclusion criteria:
Dry skin, elastic reduces, and the skin ageing phenomenons such as wrinkle, pigmentation, the colour of skin be gloomy occurs；It is willing to during whole test
Meaning avoids Exposure to Sunlight, or during contact sunlight, face uses the sunscreen product of SPF >=30；Do not use other anti-ageing during whole test
Old product.
The 540 tested volunteers of example women are randomly divided into 9 groups, respectively control group, embodiment 1-5 group, comparative example 1-3 group, often group
60 examples, wherein, control group experimenter uses Shiseido Urara Multy-Vitalizing Emulsion, and (Shiseido is swung the many vigor of Lay
Emulsion), early, evening takes appropriate emulsion and spreads upon face after face cleaning；Embodiment 1-5 group and comparative example 1-3 group experimenter make respectively
The anti-aging skin care product prepared by embodiment of the present invention 1-5, comparative example 1-3, early, evening takes appropriate skin care item and spreads upon after face cleaning
Face, the whole test period of each group is 90d, the bad reaction occurred during record test, and experimenter is carried out effect assessment.
Skin aging is assessed: evaluate (glabella line, forehead line, fish tail by observing the wrinkle in 5 tested districts of experimenter
Line, nasolabial groove, lip week wrinkle), judgment criteria: do not have wrinkle；Slight: to have the shallow wrinkle of 2-3 bar, length ＜ 1.5cm；In
Degree: have the shallow wrinkle of 2-6 bar, length ＜ 3cm；Severe: have several main wrinkles, length reaches 4cm, simultaneously with shallow wrinkle.
Safety evaluation: after primary evaluation uses product, experimenter slight discomfort, erythema, dry, itch and shouting pain etc. occurs no
A situation arises in good reaction.
Use non-invasive instrument to detect the water content of recipient site, fat content experimenter at 90d, carry out statistical analysis.
2. result of the test
The skin aging assessment of each group experimenter after table 1 off-test
| Group | There is no wrinkle (number of cases) | Slightly (number of cases) | Moderate (number of cases) | Severe (number of cases) |
| Control group | 38 | 16 | 4 | 2 |
| Embodiment 1 | 46 | 12 | 2 | 0 |
| Embodiment 2 | 43 | 14 | 3 | 0 |
| Embodiment 3 | 42 | 14 | 4 | 0 |
| Embodiment 4 | 41 | 15 | 4 | 0 |
| Embodiment 5 | 41 | 14 | 5 | 0 |
| Comparative example 1 | 32 | 19 | 5 | 4 |
| Comparative example 2 | 30 | 20 | 5 | 5 |
| Comparative example 3 | 34 | 17 | 6 | 3 |
Each bad reaction evaluation organizing experimenter during table 2 test
| Group | Without (number of cases) | Slight uncomfortable (number of cases) | Erythema (number of cases) | It is dried (number of cases) | Itch (number of cases) | Shouting pain (number of cases) |
| Control group | 45 | 7 | 2 | 2 | 1 | 3 |
| Embodiment 1 | 55 | 3 | 0 | 2 | 0 | 0 |
| Embodiment 2 | 52 | 5 | 0 | 2 | 1 | 0 |
| Embodiment 3 | 51 | 5 | 0 | 3 | 1 | 0 |
| Embodiment 4 | 49 | 6 | 0 | 4 | 1 | 0 |
| Embodiment 5 | 48 | 7 | 0 | 4 | 1 | 0 |
| Comparative example 1 | 39 | 9 | 3 | 5 | 2 | 2 |
| Comparative example 2 | 40 | 8 | 2 | 4 | 3 | 3 |
| Comparative example 3 | 42 | 9 | 3 | 2 | 2 | 2 |
Table 3 respectively organizes the water content of stratum corneum during the test of experimenter and fat content evaluation
Note: compare with 0d,*P ＜ 0.05,**P ＜ 0.01.
Shiseido Urara Multy-Vitalizing Emulsion swings Lay many vigor emulsion for Japan's Shiseido, is raw containing epidermis
The anti-aging product of new generation of the long factor, aims at the product of Chinese women preparation, it comprises triple amino acid and repairs composition, protects
Wet, brilliant white, Cucumber Extract, promote the synthesis of collagen；The problems such as the dry, low and deep of skin and wrinkle can be effectively improved,
Protect skin simultaneously, intercept extraneous harmful substance, make skin youth, brilliant white, gloss and flexible；The epidermal growth factor contained
Sub-active component, makes that skin is smooth, compacts.
This research with Japan Shiseido swing Lay many vigor emulsion as a control group, evaluates the effect of anti-aging skin care product of the present invention, tie
Fruit shows, uses the anti-aging skin care product prepared of embodiment of the present invention 1-5, does not has that subjects skin is aging reaches the order of severity,
And control group has 2 examples；The most there is not the bad reaction of erythema, shouting pain in experimenter simultaneously, and control group has 2 example experimenters to go out
Existing erythema, there is shouting pain in 3 example experimenters；Additionally, the anti-aging skin care product using embodiment of the present invention 1-5 to prepare all can be notable
Increasing subjects skin's water content of stratum corneum and fat content, wherein the anti-aging skin care product effect with embodiment 1 preparation is preferable,
It increases subjects skin's water content of stratum corneum and reaches pole significant difference, and result above shows, prepared by embodiment of the present invention 1-5
Anti-aging skin care product can significantly improve skin aging, reduces the generation of bad reaction, increases water content of stratum corneum and fat content,
And with the anti-aging skin care product best results of embodiment 1, and it is better than control group.Anti-aging skin care product of the present invention can effectively increase
Add the water content of keratoderma, replenishing water and preserving moisture, and by increasing sebum, play the effect of greasy skin.Comparative example 1 and 2
Lacking plant extracts and epidermal stem cells secretin respectively, its effect improving skin aging substantially weakens, sending out of bad reaction
Raw rate substantially increases, and increases subjects skin's water content of stratum corneum and fat content effect is not notable, show that the present invention is anti-aging and protect
Active ingredient plant extracts and epidermal stem cells secretin in skin product have mutually coordinated, the effect mutually promoted, and lack
Weary one all can cause anti-aging effect to reduce.Active ingredient in plant extracts has promotion Transdermal absorption effect, makes to resist
The active component of old and feeble skin care item is deep into dermal layer of the skin and hypodermis gives full play to repair skin, promotes skin hyperplasia ability
Effect；Epidermal stem cells secretin contains multiple many on the Skin Cell influential growth factor of activity, cell factor, activity
The various active materials such as peptide, trace element, amino acid, may advantageously facilitate skin metabolism, delay senility.Circassian bean has
Wind and heat dispersing, eliminating dampness is antipruritic, effect of moisturizing beauty treatment, and has stronger inhibitory action, comparative example to proteolytic enzyme
Plant extracts in 3 lacks this component of circassian bean so that it is anti-aging effect is slightly better than comparative example 1 and comparative example 2, but
Substantially weaken with the effectiveness comparison of embodiment 1-5, show the activity being effectively split into point by suppressing proteolytic enzyme in circassian bean
Delay epidermal stem cells secretin to decompose at endocellular metabolism, give full play to epidermal stem cells secretin and promote skin metabolism,
The effect delayed senility.Result above shows, in anti-aging skin care product prescription of the present invention, the collocation of each composition is rationally, mutual gain,
Having significant effect to improving skin aging, and bad reaction is low, security is high.
Below it is only the preferred embodiment of the present invention, it is noted that above-mentioned preferred embodiment is not construed as the present invention
Restriction, protection scope of the present invention should be as the criterion with claim limited range.Ordinary skill for the art
For personnel, without departing from the spirit and scope of the present invention, it is also possible to make some improvements and modifications, these improvements and modifications
Also should be regarded as protection scope of the present invention.
Claims (10)
1. the anti-aging skin care product containing epidermal stem cells secretin, it is characterised in that described skin care item are by following weight hundred
The component composition of proportion by subtraction meter: epidermal stem cells secretin 0.000001-0.0005%, plant extracts 2～8%, saualane 1～2%,
EDTA-2Na 0.10～0.25%, glycerine 5～10%, polyglutamic acid 0.05～1%,K350 0.06～0.12%, Pork and beans
Cool isopropyl propionate 2～6%, cetostearyl alcohol 0.5～2%, dimethyl silicone polymer 1～2%, surplus is water.
Anti-aging skin care product the most according to claim 1, it is characterised in that described skin care item are by following percentage by weight meter
Component composition: epidermal stem cells secretin 0.000125%, plant extracts 6%, saualane 1%, EDTA-2Na 0.15%,
Glycerine 8%, polyglutamic acid 0.50%,K350 0.10%, isopropyl myristate 4%, cetostearyl alcohol 1%, poly-
Dimethyl siloxane 2%, surplus is water.
3. according to the arbitrary described anti-aging skin care product of claim 1 or 2, it is characterised in that described epidermal stem cells secretin
The mixture of the various active factor of the epidermal stem cells secretion behaved.
4. according to the arbitrary described anti-aging skin care product of claim 1 or 2, it is characterised in that described plant extracts is by following
The raw material of weight percentage is prepared: circassian bean 16～28%, sherry flower 14～30%, wilsonii 12～28%, purpleflower holly bark
14～26% and raspberry 10～22%.
Anti-aging skin care product the most according to claim 4, it is characterised in that described plant extracts is by following weight percent
The raw material of meter is prepared: circassian bean 22%, sherry flower 22%, wilsonii 20%, purpleflower holly bark 20% and raspberry 16%.
Anti-aging skin care product the most according to claim 1, it is characterised in that the preparation side of described epidermal stem cells secretin
Method comprises the following steps:
(1) acquisition of human epidermal stem cell
Take surgery excision or scraping oral epithelium tissue, tissue is proceeded to containing penicillin, streptomysin and gentamicin sulphate
In D-PBSA (phosphate buffer), under the conditions of dry ice or liquid nitrogen frozen, transport laboratory back.Rinsing 2-3 time repeatedly, adds
37 DEG C of trypsase digestion 30min, shreds oral epithelium tissue, filters epithelial cell with 70-100um cell filter,
Collect filtrate.Under the conditions of 1200r/min, centrifugal 5min, abandons supernatant, soft with Defined Keratinocyte-SFM culture medium
Piping and druming, obtains cell suspension, utilizes the collagen isolated and purified human epidermal stem cell of rapid attachment method；
(2) cultivation of human epidermal stem cell
The human epidermal stem cell that step (1) obtains is seeded in containing 100U/ml penicillin and 100ug/ml streptomysin
In Defined Keratinocyte-SFM culture medium, 37 DEG C, 5%CO2Incubator hatches 12-24h, abandons culture medium, again adds
Enter fresh containing penicillin and streptomysin Defined Keratinocyte-SFM culture medium, cultivate 1-3d, abandon culture medium, add
Enter Defined Keratinocyte-SFM culture medium to continue to cultivate.When human epidermal stem cell merges close to 90%, Secondary Culture；
(2) preparation of epidermal stem cells secretin
Human epidermal stem cell is expanded 1-5 generation, collects culture supernatant, the supernatant sterilizing filter of 0.22 μm is filtered,
The filtrate merging every generation i.e. obtains epidermal stem cells secretin.
Anti-aging skin care product the most according to claim 1, it is characterised in that the preparation method of described plant extracts includes
Following steps:
Take ingredients the most in proportion, wash away impurity, crushed after being dried, cross 50～60 mesh sieves, merge meal, add medicinal material
Meal gross weight 8～12 times amount volume fraction is the ethanol of 50～70%, and Microwave Extraction 4～6 minutes, microwave power is
400～500W, Extracting temperature is 40～60 DEG C, is filtered by extract, repeats to extract 1 time by similarity condition, merges extract,
It is evaporated at 60 DEG C survey the medicinal extract that relative density is 1.05～1.15, prepares plant extracts.
8. the method for the anti-aging skin care product prepared as described in claim 1-7 is arbitrary, it is characterised in that include following step
Rapid:
Step 1: epidermal stem cells secretin, excipient, water for injection are mixed, carries out vacuum freeze drying, must freeze
Dry powder is standby；
Step 2: EDTA-2Na, polyglutamic acid and water are mixed, be heated to 80 DEG C, homogeneous 6～10min, stirring is to dissolving
Completely, material A is obtained；
Step 3: glycerine, saualane, isopropyl myristate, cetostearyl alcohol and dimethyl silicone polymer are mixed, heating
To 80～90 DEG C, homogeneous 10～15min, stirring, to dissolving completely, obtains material B；Material B it is slowly added in material A,
Stir, start cooling；
Step 4: when temperature is down to about 30 DEG C, to emulsification pot in add plant extracts,K350 and step 1
The freeze-dried powder prepared, stirs, to plate discharging.
The preparation method of anti-aging skin care product the most according to claim 8, it is characterised in that the excipient described in step 1
The mixture prepared with 1: 1 proportioning for mannitol and L-arginine.
The preparation method of anti-aging skin care product the most according to claim 8, it is characterised in that the vacuum refrigeration of step 1
Drying steps is: epidermal stem cells secretin, excipient, water for injection are mixed and is placed in-70 DEG C of freeze dryers, cold
But to less than-50 DEG C, pre-freeze 6h, vacuumize 8h, be to slowly warm up to-20 DEG C, then be warming up to 25 DEG C with the speed of 5 DEG C/h,
Heat preservation and dryness 2h, to obtain final product.
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| CN108078909A (en) * | 2018-01-12 | 2018-05-29 | 广州赛莱拉干细胞科技股份有限公司 | Stem cell toner |
| CN108078908A (en) * | 2018-01-12 | 2018-05-29 | 广州赛莱拉干细胞科技股份有限公司 | Spirulina extract stem cell smoothing toner |
| CN110548001A (en) * | 2019-09-06 | 2019-12-10 | 沈阳细胞治疗工程技术研发中心有限公司 | Repair anti-aging skin care product containing umbilical cord mesenchymal stem cell exosomes |
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| CN110548001A (en) * | 2019-09-06 | 2019-12-10 | 沈阳细胞治疗工程技术研发中心有限公司 | Repair anti-aging skin care product containing umbilical cord mesenchymal stem cell exosomes |
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| CN105853341B (en) | 2018-10-26 |
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