CN105852099A - Probiotic fungus compounding functional food - Google Patents
Probiotic fungus compounding functional food Download PDFInfo
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- CN105852099A CN105852099A CN201610232843.3A CN201610232843A CN105852099A CN 105852099 A CN105852099 A CN 105852099A CN 201610232843 A CN201610232843 A CN 201610232843A CN 105852099 A CN105852099 A CN 105852099A
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- enzymolysis
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- lactobacillus plantarum
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- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 1
- ZWLUXSQADUDCSB-UHFFFAOYSA-N phthalaldehyde Chemical compound O=CC1=CC=CC=C1C=O ZWLUXSQADUDCSB-UHFFFAOYSA-N 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 229920001184 polypeptide Polymers 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 102000004196 processed proteins & peptides Human genes 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000011321 prophylaxis Methods 0.000 description 1
- -1 pulvis Substances 0.000 description 1
- 210000004994 reproductive system Anatomy 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000008159 sesame oil Substances 0.000 description 1
- 235000011803 sesame oil Nutrition 0.000 description 1
- 238000004088 simulation Methods 0.000 description 1
- 230000003860 sleep quality Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000004317 sodium nitrate Substances 0.000 description 1
- 235000010344 sodium nitrate Nutrition 0.000 description 1
- 239000007901 soft capsule Substances 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 208000018556 stomach disease Diseases 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-N sulfuric acid Substances OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 229960003080 taurine Drugs 0.000 description 1
- 235000019165 vitamin E Nutrition 0.000 description 1
- 229940046009 vitamin E Drugs 0.000 description 1
- 239000011709 vitamin E Substances 0.000 description 1
- 229920001285 xanthan gum Polymers 0.000 description 1
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
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Abstract
The invention belongs to the field of health-care food, and particularly relates to probiotic fungus compounding functional food. The functional food comprises, by weight, 20-70 parts of tricholoma-matsutake enzymolysis powder, 5-10 parts of radix-ophiopogonis extract, 5-10 parts of lentinan, 2-10 parts of fructo-oligosaccharide, 2-10 parts of wheat fibers, 2-5 parts of soybean fibers, 0.01-0.03 part of tea polyphenol, 10-50 parts of malt extract and 20-40 parts of lactobacillus plantarum powder. By means of a polysaccharide extraction method, the polysaccharide extraction ratio is increased, the product has the good effect of improving the immunity accordingly, and the obtained product has the good effect of degrading cholesterol.
Description
Technical field:
The invention belongs to nutritional health food field, be specifically related to a kind of compound probiotic fungi functional food.
Background technology:
Matsutake is the rare famous and precious edible fungi of a kind of pure natural, is described as " king in bacterium ".Containing 18 kinds of ammonia
Base acid, 14 kinds of micro elements needed by human, 49 kinds of active nutritional materials, 5 kinds of unrighted acids, 8 kinds of dimensions
Raw element, 2 kinds of glycoprotein, abundant dietary fiber and various active enzyme, another containing 3 kinds of precious active materials,
Be double-strand blazei polysaccharide respectively, the unique cancer-resisting substance of matsutake polypeptide and the whole world--matsutakealcohol, is the world
Upper most precious natural medical fungus.Matsutake is the most balanced in nutrition, sufficient, but also is improved immunity, resists
Cancer is antitumor, treatment diabetes and angiocardiopathy, anti-aging nursing face, rush stomach protect the liver the multiple efficacies such as dirty.
Matsutake is the natural nourishing category of globalization.
Fungi polysaccharide has extremely strong repair to cell.Take fungi polysaccharide health food, can supplement in time
The materials such as the polysaccharide lacked in cytoplasm, make impaired cell be repaired timely, reach to keep cell to be good for
Health, extend cell survival thus realize the purpose of health.Shanghai Medical Univ Li Rui etc. test discovery,
Fungi polysaccharide can significantly improve mobility and the closed stratum of cell membrane, after old and feeble cell is cultivated with fungi polysaccharide,
Closed stratum raising 11%, mobility raising 32%, cell membrane fluidity, the raising of closure, show cell
Physiological function is improved.
Modern science and technology shows, fungi polysaccharide is most important effective component contained in edible mushroom.Be a kind of β-
The polysaccharide of type, has pharmacologically active widely.Glossy ganoderma tuber of dwarf lilyturf, Cordyceps sinensis, auricularia auriculajudae, white fungus, mushroom, monkey
The edible mushrooms such as head mushroom, Pleurotus nebrodensis, tabasheer, rainbow conk, coprinus comatus, matsutake, Phellinus, all contain fungi polysaccharide.
Chinese invention patent 201110407414.2 discloses a kind of mycotrophy liquid with health role, should
Nutrient solution with fungies such as mushroom, grifola frondosus, mushroom, Hericium erinaceus, rainbow conk as primary raw material, be aided with xylo-oligosaccharide,
The nutrients such as isomalt, taurine, lysine, vitamin E, xanthans and water process, former
Material compatibility science, synergy, there is raising body immunity and promote that body gastric mucosa injury repair is difunctional
Health-care effect.
Probio, is the active microorganism that a class is useful to host, is to be colonizated in human body intestinal canal, reproductive system,
Definite health efficacy can be produced thus improve host's microecological balance, the active beneficial microbe of performance beneficial effect
General name.It is widely used in bioengineering, industrial or agricultural, food security and life and health field.
Regulation functions of intestines and stomach disorder is one of function of being widely known by the people most of probio, and in addition, probio also has
Have and produce nutriment, the infection of opposing bacterial virus and the function of some disease of prophylactic treatment.The present invention will
A kind of composite fungi amylose composition being added with probio is provided, has fungi and the merit of polysaccharide raising immunity concurrently
Energy and the health-care efficacy of probio, improve the current problem that health products effect is single, complex function is undesirable.
Summary of the invention:
In order to solve the problems referred to above, the present invention provides a kind of compound probiotic fungi functional food, by following heavy
The raw material composition of amount number:
Matsutake enzymolysis powder 20-70 part, ophiopogon japonicus extract 5-10 part, lentinan 5-10 part, FOS 2-10
Part, Semen Tritici aestivi fiber 2-10 part, fibre and soya 2-5 part, Tea Polyphenols 0.01-0.03 part, malt extract 10-50 part,
Lactobacillus plantarum pulvis 20-40 part.
The preparation method of described matsutake enzymolysis powder is as follows:
(1) Tricholoma matsutake (lto et lmai) Singer sporophore drying and crushing；
(2) water-soluble homogeneous: the Tricholoma matsutake (lto et lmai) Singer sporophore after pulverizing adds in stainless steel cylinder, adds fructification quality
The water of 3-6 times, soaks 3-5 hour, then by this Tricholoma matsutake (lto et lmai) Singer sporophore liquid by colloid mill, colloid mill operation bar
Part is: the gap adjusting colloid mill stator and rotor is 0.5-1 micron, and colloid mill flow is 0.4-1 ton/little
Time；
(3) intensification enzymolysis: the Tricholoma matsutake (lto et lmai) Singer sporophore liquid through milling treatment of colloid is transferred in stainless steel enzymatic vessel
It is heated to 50-60 DEG C, adjusts pH to 4.5-6.0, add the cellulose of Tricholoma matsutake (lto et lmai) Singer sporophore weight 0.05-0.1%
Enzyme, the 1,4 beta-glucanase of 0.01-0.1%, the protease of 0.01-0.1%, insulation enzymolysis 0.5-1.5 hour,
Enzymolysis process is stirred continuously；
(4) it is dried: be dried after the mash filtrations after enzymolysis and obtain matsutake enzymolysis powder；
Described ophiopogon japonicus extract preparation method is as follows:
The tuber of dwarf lilyturf pulverizes, and after crossing 40-50 mesh sieve, adds 3-6 times of weight absolute ethyl alcohol Soakage extraction tuber of dwarf lilyturf, controls
Temperature 30-45 DEG C, after 2-4 hour adjust temperature be 55-60 DEG C 1-2 hour, extract concentrate, be dried
To ethanol extract；The tuber of dwarf lilyturf after alcohol extract adds 75-85 DEG C of hot water in residue, and hot water addition is the tuber of dwarf lilyturf
2-4 times of residue weight, processes 30-50 minute time, extracts 2-3 time continuously, is concentrated in vacuo by extract
Rear spray drying, obtains hot water extract；Above-mentioned ethanol extract and hot water extract are merged pulverizing, mistake
60 mesh sieves, obtain ophiopogon japonicus extract；
Described lentinan extracting method is as follows:
(1) take after commercially available mushroom fruiting body dries pulverizing and cross 80 mesh sieves；
(2) ultrasonic hot water extraction: by the mushroom fruiting body after sieving and the ratio of water quality volume ratio 1-3:20
Example adds pure water, under the conditions of pH7,400W ultrasonic wave, 105 DEG C of water-bath extraction 0.5h；
(3) vapours extraction: said extracted system is placed in autoclave, 121 DEG C, 5min；
(4) circulation repeat step (2), (3) 4 times；
(5) centrifugation obtains supernatant；
(6) supernatant reduced pressure concentration obtains concentrate, adds 95% ethanol of 3 times of volumes of concentrate, at 4 DEG C
Alcohol analysis 24h, abandoning supernatant, after adding absolute ethyl alcohol isopyknic with initial concentration liquid standing 30min,
Abandoning supernatant, washing of precipitate 3 times, it is spray-dried, obtains lentinan.
Described Lactobacillus plantarum pulvis is prepared by Lactobacillus plantarum (Lactobacillus plantarum) tlj-2015,
This bacterium is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms on November 30th, 2015
CGMCC (is called for short) in center, and preserving number is CGMCC NO.11763, and preservation address is: city of BeiJing, China
North Star West Road, Chaoyang District 1 institute 3, Institute of Microorganism, Academia Sinica, postcode: 100101；
In described Lactobacillus plantarum pulvis, viable bacteria content is: 7 × 1012-9×1012cfu/g；
The preparation method of described Lactobacillus plantarum pulvis is as follows:
(1) take Lactobacillus plantarum CGMCC NO.11763 mono-ring on inclined-plane, access and cultivate equipped with 50mL
In the 250mL triangular flask of base MRS (without agar) (concentration of glucose is 150g/L) culture medium, 200rpm,
Cultivate 12-16h, make thalline be in mid log phase for 37 DEG C；
(2) being accessed in fermentation medium by the bacterial classification of logarithmic phase, inoculum concentration is at 10-15%, 35-38 DEG C
100-200rpm cultivates 8-12 hour, and dissolved oxygen controls 10% (ventilation 0.5L/min), Anaerobic culturel the most again
60-70 hour；The complete zymotic fluid that ferments staticly settles, centrifugal be precipitated thing, then adds sediment quality 1
Carrier again, mixes, and 50 DEG C of fluidized bed dryings i.e. obtain Lactobacillus plantarum pulvis, and vehicle weight number forms
For: sour milk powder 25 parts, 12 parts of dextrin.
Described fermentation medium composition is as follows: peptone 10-12g, beef extract 10-12g, yeast extract 5-8
G, diammonium hydrogen citrate 2-4g, glucose 20-25g, Tween 80 1-2mL, sodium acetate 5-7g, phosphoric acid
Hydrogen dipotassium 2-3g, magnesium sulfate 0.58-0.6g, manganese sulfate 0.25-0.3g, cholesterol 100-120mg, Chinese herbal medicine
Pulvis 5-8g, distilled water 1 000mL, pH 6.2～6.6；
The adding method of described cholesterol is: first use anhydrous alcohol solution cholesterol, and compound concentration is
The cholesterol solution of 10-12mg/mL, adds in culture medium the most by a certain percentage, makes cholesterol ultimate density
For 100-120 μ g/mL；
The preparation method of described Chinese herbal medicine powder is as follows:
Weigh fruit of Chinese magnoliavine 10-20 part；Radix Codonopsis 10-15 part；Hawthorn 10-20 part；Respectively by said herbal medicine powder
Being broken to particle diameter is less than 2 millimeters, then uniformly mixes and add the water of 3-6 times of weight in container, controls temperature
Spending 45-60 DEG C and keep 2～4h, add mixing enzyme preparation and carry out enzymolysis, regulation pH value is 6-7, enzymolysis 2-4h,
Finally add 0.5-3 times of w ethanol of mixed material and the mixture of propyl alcohol, ultrasonic extraction under 110W power
0.5～1.5h, filter；Filter vacuum concentrates postlyophilization and obtains Chinese herbal medicine powder；
Described mixed enzyme addition is the 5-10% of mixed material gross weight；
The parts by weight of described mixed enzyme consist of: zytase 15-20 part, beta amylase 10-15 part, pectin
Enzyme 10-15 part, acid protease 10-15 part, acid phosphatase 5-10 part；
The mass ratio of described ethanol and propyl alcohol is 1:1-2.
The preparation method of described compound probiotic fungi functional food is: be processed as sheet by this area conventional method
Agent, pulvis, granule, soft capsule, hard shell capsules etc., directly take, it is also possible to use as raw-food material.
Beneficial effect:
1, except containing matsutake, the tuber of dwarf lilyturf, mushroom in compound probiotic fungi functional food provided by the present invention
Polysaccharide is outer possibly together with Lactobacillus plantarum CGMCC NO.11763, and this bacterial strain 1. degrading nitrite speed is fast,
Capacity of decomposition reaches 10.9mg/h/kg, can effectively decompose people due to nitrite that is improper diet and that take in；
2. this bacterium is high to degrading rate of cholesterol, can reach 64.76%, is particularly suited for obese people and three high patients；
3. this bacterium Adhering capacity is high, and mensuration is 95.71% from aggegation rate, can effectively be colonizated in gastrointestinal system,
Give full play to its physiological activity.
2, in Lactobacillus plantarum cultural method provided by the present invention, fermentation medium is added with traditional Chinese medicine ingredients
The fruit of Chinese magnoliavine, Radix Codonopsis and hawthorn, the interpolation of these three important component can be effectively improved the acidproof resistance to courage of Lactobacillus plantarum
Salt ability, thus improve its effect played in human body intestinal canal.
3, in Lactobacillus plantarum cultural method provided by the present invention, fermentation medium adds with soluble form
Added with cholesterol, the ability of strains for degrading cholesterol after having fermented, can be effectively improved.Due to present invention offer
Product is added with described Lactobacillus plantarum so that the present invention is in addition to having raising body immunity ability, also
There is the ability of preferable cholesterol degradation.
Detailed description of the invention:
Embodiment 1: a kind of compound probiotic fungi functional food
A kind of compound probiotic fungi functional food, is made up of the raw material of following parts by weight:
25 parts of matsutake enzymolysis powder, ophiopogon japonicus extract 5 parts, lentinan 5 parts, FOS 2 parts, wheat
Fiber 2 parts, fibre and soya 2 parts, Tea Polyphenols 0.01 part, malt extract 10 parts, 20 parts of Lactobacillus plantarum pulvis.
The preparation method of described matsutake enzymolysis powder is as follows:
(1) Tricholoma matsutake (lto et lmai) Singer sporophore drying and crushing；
(2) water-soluble homogeneous: the Tricholoma matsutake (lto et lmai) Singer sporophore after pulverizing adds in stainless steel cylinder, adds fructification quality
The water of 3 times, soaks 3 hours, and then by this Tricholoma matsutake (lto et lmai) Singer sporophore liquid by colloid mill, colloid mill operation condition is:
The gap adjusting colloid mill stator and rotor is 0.5 micron, and colloid mill flow is 0.4 ton hour；
(3) intensification enzymolysis: the Tricholoma matsutake (lto et lmai) Singer sporophore liquid through milling treatment of colloid is transferred in stainless steel enzymatic vessel
Be heated to 50 DEG C, adjust pH to 4.5, add the cellulase of Tricholoma matsutake (lto et lmai) Singer sporophore weight 0.05%, 0.01%
1,4 beta-glucanase, the protease of 0.01%, insulation enzymolysis 0.5 hour, enzymolysis process is stirred continuously；
(4) it is dried: be dried after the mash filtrations after enzymolysis and obtain matsutake enzymolysis powder；
Described ophiopogon japonicus extract preparation method is as follows:
The tuber of dwarf lilyturf pulverizes, and after crossing 40 mesh sieves, adds 3 times of weight absolute ethyl alcohol Soakage extraction tuber of dwarf lilyturf, controls temperature
30 DEG C, after 2 hours adjust temperature be 55 DEG C 1 hour, extract concentrate, be dried to obtain ethanol extract；
The tuber of dwarf lilyturf after alcohol extract adds 75 DEG C of hot water in residue, and hot water addition is the tuber of dwarf lilyturf 2 times of residue weight,
30 minutes process time, extract 2 times continuously, be spray-dried after extract is concentrated in vacuo, obtain hot water and carry
Take thing；Above-mentioned ethanol extract and hot water extract are merged pulverizing, crosses 60 mesh sieves, obtain ophiopogon japonicus extract；
Described lentinan extracting method is as follows:
(1) take after commercially available mushroom fruiting body dries pulverizing and cross 80 mesh sieves；
(2) ultrasonic hot water extraction: in the ratio of the mushroom fruiting body after sieving Yu water quality volume ratio 1:20
Add pure water, under the conditions of pH7,400W ultrasonic wave, 105 DEG C of water-bath extraction 0.5h；
(3) vapours extraction: said extracted system is placed in autoclave, 121 DEG C, 5min；
(4) circulation repeat step (2), (3) 4 times；
(5) centrifugation obtains supernatant；
(6) supernatant reduced pressure concentration obtains concentrate, adds 95% ethanol of 3 times of volumes of concentrate, at 4 DEG C
Alcohol analysis 24h, abandoning supernatant, after adding absolute ethyl alcohol isopyknic with initial concentration liquid standing 30min,
Abandoning supernatant, washing of precipitate 3 times, it is spray-dried, obtains lentinan.
The preparation method of described Lactobacillus plantarum pulvis is as follows:
(1) take Lactobacillus plantarum CGMCC NO.11763 mono-ring on inclined-plane, access and cultivate equipped with 50mL
In the 250mL triangular flask of base MRS (without agar) (concentration of glucose is 150g/L) culture medium, 200rpm,
Cultivate 12h, make thalline be in mid log phase for 37 DEG C；
(2) being accessed in fermentation medium by the bacterial classification of logarithmic phase, inoculum concentration is 10%, 100rpm at 35 DEG C
Cultivating 8 hours, dissolved oxygen controls 10% (ventilation 0.5L/min), Anaerobic culturel 60 hours the most again；Ferment
Complete zymotic fluid staticly settles, centrifugal be precipitated thing, then adds the carrier of sediment quality 1 times, mixing
Uniformly, 50 DEG C of fluidized bed dryings i.e. obtain Lactobacillus plantarum pulvis, and vehicle weight number consists of: sour milk powder 25
Part, 12 parts of dextrin.
Described fermentation medium composition is as follows: peptone 10g, beef extract 10g, yeast extract 5g, lemon
Lemon acid hydrogen two ammonium 2g, glucose 20g, Tween 80 1mL, sodium acetate 5g, dipotassium hydrogen phosphate 2g, sulfuric acid
Magnesium 0.58g, manganese sulfate 0.25g, cholesterol 100mg, Chinese herbal medicine powder 5g, distilled water 1 000mL, pH
6.2；
The adding method of described cholesterol is: first use anhydrous alcohol solution cholesterol, and compound concentration is 10mg/mL
Cholesterol solution, add to the most by a certain percentage in culture medium, making cholesterol ultimate density is 100 μ g/mL；
The preparation method of described Chinese herbal medicine powder is as follows:
Weigh 10 parts of the fruit of Chinese magnoliavine；Radix Codonopsis 10 parts；Hawthorn 10 parts；Respectively said herbal medicine is crushed to particle diameter
It is less than 2 millimeters, in container, then uniformly mixes and add the water of 3 times of weight, control temperature 45 C and protect
Holding 2h, add mixing enzyme preparation and carry out enzymolysis, regulation pH value is 6, and enzymolysis 2h finally adds mixed material
0.5 times of w ethanol and the mixture of propyl alcohol, ultrasonic extraction 0.5h under 110W power, filters；Filter vacuum
Concentrate postlyophilization and obtain Chinese herbal medicine powder；
Described mixed enzyme addition is the 5% of mixed material gross weight；
The parts by weight of described mixed enzyme consist of: zytase 15 parts, beta amylase 10 parts, pectase 10 parts,
Acid protease 10 parts, acid phosphatase 5 parts；
The mass ratio of described ethanol and propyl alcohol is 1:1.
2 one kinds of compound probiotic fungi functional foods of embodiment
A kind of compound probiotic fungi functional food, is made up of the raw material of following parts by weight:
70 parts of matsutake enzymolysis powder, ophiopogon japonicus extract 10 parts, lentinan 10 parts, FOS 10 parts is little
Wheat fiber 10 parts, fibre and soya 5 parts, Tea Polyphenols 0.03 part, malt extract 50 parts, Lactobacillus plantarum pulvis 40
Part.
The preparation method of described matsutake enzymolysis powder is as follows:
(1) Tricholoma matsutake (lto et lmai) Singer sporophore drying and crushing；
(2) water-soluble homogeneous: the Tricholoma matsutake (lto et lmai) Singer sporophore after pulverizing adds in stainless steel cylinder, adds fructification quality
The water of 6 times, soaks 5 hours, and then by this Tricholoma matsutake (lto et lmai) Singer sporophore liquid by colloid mill, colloid mill operation condition is:
The gap adjusting colloid mill stator and rotor is 1 micron, and colloid mill flow is 1 ton hour；
(3) intensification enzymolysis: the Tricholoma matsutake (lto et lmai) Singer sporophore liquid through milling treatment of colloid is transferred in stainless steel enzymatic vessel
Be heated to 50-60 DEG C, adjust pH to 6.0, add the cellulase of Tricholoma matsutake (lto et lmai) Singer sporophore weight 0.1%, 0.1%
1,4 beta-glucanase, the protease of 0.1%, insulation enzymolysis 1.5 hours, enzymolysis process is stirred continuously；
(4) it is dried: be dried after the mash filtrations after enzymolysis and obtain matsutake enzymolysis powder；
Described ophiopogon japonicus extract preparation method is as follows:
The tuber of dwarf lilyturf pulverizes, and after crossing 50 mesh sieves, adds 6 times of weight absolute ethyl alcohol Soakage extraction tuber of dwarf lilyturf, controls temperature
45 DEG C, after 4 hours adjust temperature be 60 DEG C 2 hours, extract concentrate, be dried to obtain ethanol extract；
The tuber of dwarf lilyturf after alcohol extract adds 85 DEG C of hot water in residue, and hot water addition is the tuber of dwarf lilyturf 4 times of residue weight,
50 minutes process time, extract 3 times continuously, be spray-dried after extract is concentrated in vacuo, obtain hot water and carry
Take thing；Above-mentioned ethanol extract and hot water extract are merged pulverizing, crosses 60 mesh sieves, obtain ophiopogon japonicus extract；
Described lentinan extracting method is as follows:
(1) take after commercially available mushroom fruiting body dries pulverizing and cross 80 mesh sieves；
(2) ultrasonic hot water extraction: in the ratio of the mushroom fruiting body after sieving Yu water quality volume ratio 3:20
Add pure water, under the conditions of pH7,400W ultrasonic wave, 105 DEG C of water-bath extraction 0.5h；
(3) vapours extraction: said extracted system is placed in autoclave, 121 DEG C, 5min；
(4) circulation repeat step (2), (3) 4 times；
(5) centrifugation obtains supernatant；
(6) supernatant reduced pressure concentration obtains concentrate, adds 95% ethanol of 3 times of volumes of concentrate, at 4 DEG C
Alcohol analysis 24h, abandoning supernatant, after adding absolute ethyl alcohol isopyknic with initial concentration liquid standing 30min,
Abandoning supernatant, washing of precipitate 3 times, it is spray-dried, obtains lentinan.
The preparation method of described Lactobacillus plantarum pulvis is as follows:
(1) take Lactobacillus plantarum CGMCC NO.11763 mono-ring on inclined-plane, access and cultivate equipped with 50mL
In the 250mL triangular flask of base MRS (without agar) (concentration of glucose is 150g/L) culture medium, 200rpm,
Cultivate 16h, make thalline be in mid log phase for 37 DEG C；
(2) being accessed in fermentation medium by the bacterial classification of logarithmic phase, inoculum concentration is 15%, 200rpm at 38 DEG C
Cultivating 12 hours, dissolved oxygen controls 10% (ventilation 0.5L/min), Anaerobic culturel 70 hours the most again；Fermentation
Complete zymotic fluid staticly settles, centrifugal be precipitated thing, then adds the carrier of sediment quality 1 times, mixed
Closing uniformly, 50 DEG C of fluidized bed dryings i.e. obtain Lactobacillus plantarum pulvis, and vehicle weight number consists of: sour milk powder 25
Part, 12 parts of dextrin.
Described fermentation medium composition is as follows: peptone 12g, beef extract 12g, yeast extract 8g, lemon
Acid hydrogen two ammonium 4g, glucose 25g, Tween 80 2mL, sodium acetate 7g, dipotassium hydrogen phosphate 3g, sulfuric acid
Magnesium 0.6g, manganese sulfate 0.3g, cholesterol 120mg, Chinese herbal medicine powder 8g, distilled water 1000mL, pH 6.6；
The adding method of described cholesterol is: first use anhydrous alcohol solution cholesterol, and compound concentration is 12mg/mL
Cholesterol solution, add to the most by a certain percentage in culture medium, making cholesterol ultimate density is 120 μ g/mL；
The preparation method of described Chinese herbal medicine powder is as follows:
Weigh 20 parts of the fruit of Chinese magnoliavine；Radix Codonopsis 15 parts；Hawthorn 20 parts；Respectively said herbal medicine is crushed to particle diameter
It is less than 2 millimeters, in container, then uniformly mixes and add the water of 6 times of weight, control temperature 60 C and protect
Holding 4h, add mixing enzyme preparation and carry out enzymolysis, regulation pH value is 7, and enzymolysis 4h finally adds mixed material
3 times of w ethanol and the mixture of propyl alcohol, ultrasonic extraction 1.5h under 110W power, filters；Filter vacuum is dense
Contracting postlyophilization obtains Chinese herbal medicine powder；
Described mixed enzyme addition is the 10% of mixed material gross weight；
The parts by weight of described mixed enzyme consist of: zytase 20 parts, beta amylase 15 parts, pectase 15 parts,
Acid protease 15 parts, acid phosphatase 10 parts；
The mass ratio of described ethanol and propyl alcohol is 1:2.
Embodiment 3: a kind of compound probiotic fungi functional food
A kind of compound probiotic fungi functional food, is made up of the raw material of following parts by weight:
50 parts of matsutake enzymolysis powder, ophiopogon japonicus extract 8 parts, mushroom lentinan 8 parts, FOS 7 parts,
Semen Tritici aestivi fiber 7 parts, fibre and soya 3 parts, Tea Polyphenols 0.02 part, malt extract 30 parts, Lactobacillus plantarum pulvis 30
Part.
The preparation method of described matsutake enzymolysis powder is as follows:
(1) Tricholoma matsutake (lto et lmai) Singer sporophore drying and crushing；
(2) water-soluble homogeneous: the Tricholoma matsutake (lto et lmai) Singer sporophore after pulverizing adds in stainless steel cylinder, adds fructification quality
The water of 5 times, soaks 4 hours, and then by this Tricholoma matsutake (lto et lmai) Singer sporophore liquid by colloid mill, colloid mill operation condition is:
The gap adjusting colloid mill stator and rotor is 0.8 micron, and colloid mill flow is 0.8 ton hour；
(3) intensification enzymolysis: the Tricholoma matsutake (lto et lmai) Singer sporophore liquid through milling treatment of colloid is transferred in stainless steel enzymatic vessel
Be heated to 55 DEG C, adjust pH to 5.0, add the cellulase of Tricholoma matsutake (lto et lmai) Singer sporophore weight 0.08%, 0.08%
1,4 beta-glucanase, the protease of 0.08%, insulation enzymolysis 1 hour, enzymolysis process is stirred continuously；
(4) it is dried: be dried after the mash filtrations after enzymolysis and obtain matsutake enzymolysis powder；
Described ophiopogon japonicus extract preparation method is as follows:
The tuber of dwarf lilyturf pulverizes, and after crossing 45 mesh sieves, adds 3-6 times of weight absolute ethyl alcohol Soakage extraction tuber of dwarf lilyturf, controls temperature
Spend 40 DEG C, after 3 hours adjust temperature be 60 DEG C 2 hours, extract concentrate, be dried to obtain ethanol extract；
The tuber of dwarf lilyturf after alcohol extract adds 80 DEG C of hot water in residue, and hot water addition is the tuber of dwarf lilyturf 3 times of residue weight,
40 minutes process time, extract 3 times continuously, be spray-dried after extract is concentrated in vacuo, obtain hot water and carry
Take thing；Above-mentioned ethanol extract and hot water extract are merged pulverizing, crosses 60 mesh sieves, obtain ophiopogon japonicus extract；
Described lentinan extracting method is as follows:
(1) take after commercially available mushroom fruiting body dries pulverizing and cross 80 mesh sieves；
(2) ultrasonic hot water extraction: in the ratio of the mushroom fruiting body after sieving Yu water quality volume ratio 2:20
Add pure water, under the conditions of pH7,400W ultrasonic wave, 105 DEG C of water-bath extraction 0.5h；
(3) vapours extraction: said extracted system is placed in autoclave, 121 DEG C, 5min；
(4) circulation repeat step (2), (3) 4 times；
(5) centrifugation obtains supernatant；
(6) supernatant reduced pressure concentration obtains concentrate, adds 95% ethanol of 3 times of volumes of concentrate, at 4 DEG C
Alcohol analysis 24h, abandoning supernatant, after adding absolute ethyl alcohol isopyknic with initial concentration liquid standing 30min,
Abandoning supernatant, washing of precipitate 3 times, it is spray-dried, obtains lentinan.
The preparation method of described Lactobacillus plantarum pulvis is as follows:
(1) take Lactobacillus plantarum CGMCC NO.11763 mono-ring on inclined-plane, access and cultivate equipped with 50mL
In the 250mL triangular flask of base MRS (without agar) (concentration of glucose is 150g/L) culture medium, 200rpm,
Cultivate 12-16h, make thalline be in mid log phase for 37 DEG C；
(2) being accessed in fermentation medium by the bacterial classification of logarithmic phase, inoculum concentration is 12%, 150rpm at 37 DEG C
Cultivating 10 hours, dissolved oxygen controls 10% (ventilation 0.5L/min), Anaerobic culturel 65 hours the most again；Fermentation
Complete zymotic fluid staticly settles, centrifugal be precipitated thing, then adds the carrier of sediment quality 1 times, mixed
Closing uniformly, 50 DEG C of fluidized bed dryings i.e. obtain Lactobacillus plantarum pulvis, and vehicle weight number consists of: sour milk powder 25
Part, 12 parts of dextrin.
Described fermentation medium composition is as follows: peptone 11g, beef extract 11g, yeast extract 6g, lemon
Lemon acid hydrogen two ammonium 3g, glucose 22g, Tween 80 1.5mL, sodium acetate 6g, dipotassium hydrogen phosphate 2.5g,
Magnesium sulfate 0.6g, manganese sulfate 0.25g, cholesterol 110mg, Chinese herbal medicine powder 6g, distilled water 1 000mL,
pH 6.5；
The adding method of described cholesterol is: first use anhydrous alcohol solution cholesterol, and compound concentration is 11mg/mL
Cholesterol solution, add to the most by a certain percentage in culture medium, making cholesterol ultimate density is 110 μ g/mL；
The preparation method of described Chinese herbal medicine powder is as follows:
Weigh 15 parts of the fruit of Chinese magnoliavine；Radix Codonopsis 12 parts；Hawthorn 15 parts；Respectively said herbal medicine is crushed to particle diameter
It is less than 2 millimeters, in container, then uniformly mixes and add the water of 5 times of weight, control temperature 50 C and protect
Holding 3h, add mixing enzyme preparation and carry out enzymolysis, regulation pH value is 6.5, and enzymolysis 3h finally adds mixture
Expect 2 times of w ethanol and the mixture of propyl alcohol, ultrasonic extraction 1h under 110W power, filter；Filter vacuum is dense
Contracting postlyophilization obtains Chinese herbal medicine powder；
Described mixed enzyme addition is the 8% of mixed material gross weight；
The parts by weight of described mixed enzyme consist of: zytase 18 parts, beta amylase 12 parts, pectase 12 parts,
Acid protease 12 parts, acid phosphatase 8 parts；
The mass ratio of described ethanol and propyl alcohol is 1:1.5.
Embodiment 4 effect experimental
1, the cultivation of Lactobacillus plantarum CGMCC NO.11763
(1) take Lactobacillus plantarum CGMCC NO.11763 mono-ring on inclined-plane, access and cultivate equipped with 50mL
In the 250mL triangular flask of base MRS (without agar) (concentration of glucose is 150g/L) culture medium, 200rpm,
Cultivate about 12h, make thalline be in mid log phase for 37 DEG C.
(2) bacterial classification of logarithmic phase is accessed equipped with in the 5L fermentation tank of fermentation medium.Inoculum concentration is 10%,
At 37 DEG C, 100rpm cultivates 8 hours, and logarithm early stage dissolved oxygen controls 10% (ventilation 0.5L/min), and the later stage detests
Oxygen is cultivated 63 hours.
Fermentation medium:
1.: peptone 11g, beef extract 11g, yeast extract 6g, diammonium hydrogen citrate 3g, grape
Sugar 22g, Tween 80 1.5mL, sodium acetate 6g, dipotassium hydrogen phosphate 2.5g, magnesium sulfate 0.6g, manganese sulfate
0.25g, cholesterol 110mg, Chinese herbal medicine powder 6g, distilled water 1 000mL, pH 6.5；
2.: the cholesterol in leaving out 1.；
3.: the Chinese herbal medicine powder in leaving out 1.；
2, cholesterol degradation ability measures
After 2. 1. step 1 and complete fermentation for fermentation medium with fermentation medium respectively, take 1ml respectively
Zymotic fluid is resuspended in 1ml sterilized water after being centrifuged and washing 2 times with sterilized water, is inoculated in 10mL's respectively
In MRS cholesterol fluid nutrient medium (cholesterol level 0.1mg/ml, pH 6.2), the constant temperature of 37 DEG C stands respectively
Cultivate 20h, 40h, 60h standby, take bacteria liquid sample each 1ml, the 9000r/min of above cultivation different time,
At 4 DEG C, centrifugal 10min, obtains fermented supernatant fluid, and o-phthalaldehyde method measures cholesterol level (tool in supernatant
Body is: takes each supernatant 0.1ml in corresponding test tube, adds glacial acetic acid 0.3ml, the O-phthalic of 1mg/ml
Aldehyde 0.15ml, is slowly added into concentrated sulfuric acid 1.0ml, mixes.Room temperature stands 10min, surveys under 550nm
Light absorption value).Each process 3 repetition, in kind makes cholesterol calibration curve, calculates in supernatant
Cholesterol level and degradation rate, the results are shown in Table 1.Understanding, cholesterol is had well by CGMCC NO.11763
Degradation, after 60h hour, degradation rate can reach 64.76%.Further, the culture medium of cholesterol it is added with
The ability of the CGMCC NO.11763 cholesterol degradation turned out significantly improves.
The table 1 degraded situation to cholesterol.
3, bile tolerance experiment
After 3. 1. step 1 and complete fermentation for fermentation medium with fermentation medium respectively, take 1ml respectively
Zymotic fluid is resuspended in 1ml sterilized water after being centrifuged and washing 2 times with sterilized water, is inoculated in respectively containing various biliary
10mL MRS fluid nutrient medium (pH=6.4) of salt (concentration gradients is 0.0%, 0.4%, 0.6%, 1%),
Be placed at 37 DEG C and cultivate 0 respectively, 2,4h, each process 3 repetition.Respectively take 1ml sample bacterium solution raw in 9ml
Reason salt solution mixes, prepares dilution factor solution, take 0.1ml dilution and be coated with in MRS, in 37 DEG C of biochemistry
Incubator is inverted to cultivate the bacterium that 48 hours (each dilution factor do 3 parallel) record calculates on flat board several
Number.The results are shown in Table 2.Understand this bacterium increment of bacterium after gallbladder salinity is 1% process 4h still to reach
0.51±0.92×107(cfu/ml), there is good bile tolerance ability, and use the cultivation being added with medicinal herb components
Base, its bile tolerance ability is effectively improved.
Table 2 bile tolerance ability detection (× 107cfu/ml)
4, acidproof experiment
After 3. 1. step 1 and complete fermentation for fermentation medium with fermentation medium respectively, take 1ml respectively
Zymotic fluid is resuspended in 1ml sterilized water after being centrifuged and washing 2 times with sterilized water, is inoculated in different pH value respectively
The 10mL MRS fluid nutrient medium of (pH gradient is 1.5,2.0,2.5,3.0,3.5,4.0), is placed in
Cultivate 0 at 37 DEG C respectively, 2,4h, each process 3 repetition.Respectively take 1ml sample bacterium solution raw in 9ml
Reason salt solution mixes, prepares dilute solution, take 0.1ml dilution and be coated with in MRS, in 37 DEG C of biochemistry
Incubator is inverted the bacterium colony number cultivated on 48 hours (each dilution factor do 3 parallel) record flat board.
The results are shown in Table 3.Illustrate that this bacterium has the strongest acid-fast ability, and the culture medium being added with Chinese herbal medicine composition can have
Effect improves the acid resistance of this bacterium.
Table 3 acid-fast ability detection (× 107cfu/ml)
5, nitrite decomposition experiment
With 1. culture medium in step 1, in sweat, wear rate stream according to nitrite adds the Asia of 20g/L
Sodium nitrate solution, cultivates 2-3 days.After fermentation ends, calculate sweat Lactobacillus plantarum CGMCC
The NO.11763 degradation rate to natrium nitrosum.Found that: under this condition, CGMCC NO.11763
The degradation rate of natrium nitrosum can be reached 653mg/h/L.
6, Adhering capacity measures
Cultivate CGMCC NO.11763 (MRS fluid nutrient medium), bacillus coli DH 5 alpha (LB liquid
Culture medium) 24h obtains zymotic fluid, and it is respectively placed in 3000r/min, centrifugal 10min at 4 DEG C, collects bacterium mud,
(i.e. add in bacterium colony for 2 times with sterile phosphate buffer (PBS) the washing bacterium mud of pH=7.0 respectively
PBS, after concussion mixes, is placed in 3000r/min, centrifugal 10min at 4 DEG C, collects thalline).Self-solidifying
Collection rate (%): bacterium mud CGMCC NO.11763 is formed at wavelength 600nm with aseptic PBS
Light absorption value is suspension bacteria liquid and the bacteria suspension of 0.4 ± 0.1 (A 0), measures light absorption value A 24 after standing 24h,
It is (A 0 A 24)/A 0 from aggegation rate (%) formula.Measurement result is shown in Table 5, it is known that CGMCC
NO.11763 is 95.71% from aggegation rate, has the strongest Adhering capacity.
Table 5 Adhering capacity table
| Classification | A0 mean value±s | A24 mean values±s | Aggegation rate % |
| 11763 phosphate mixed liquors | 0.5131±0.0045 | 0.0220±0.0369 | 95.71 |
The probiotic test of embodiment 5 present invention
The product embodiment of the present invention 3 prepared, being prepared as viable count with sterilized water is 2 × 1010CFU/mL's
Probiotic solution, saves backup in 4 DEG C；
1, SGF and the resistance test of intestinal juice:
The hydrochloric acid 16.4mL taking 100g/L adds distilled water diluting, makes pH value be respectively 1.5,2.5 and 3.5,
Take 100mL dilute hydrochloric acid solution, be separately added into 1g pepsin so that it is fully dissolve, obtain SGF,
Miillpore filter degerming (0.22 μm) is standby.
Taking potassium dihydrogen phosphate 6.8g, the 500mL that adds water makes dissolving, regulates with 0.1moL/L sodium hydroxide solution
PH value is to 6.8；Separately taking trypsase 10g, the 100mL that adds water makes dissolving, after two liquid mixing, adds water
Being diluted to 1000ml, obtain simulated intestinal fluid, miillpore filter degerming (0.22 μm) is standby.
Take the probiotic solution that 1mL keeps and join in the SGF of 9mL that (i.e. ten times the dilutest
Release), and the most fully mix rapidly, it is subsequently placed in 30-45 DEG C of quiescent culture 2-4h.Respectively 1h,
Take out nutrient solution when of 2h, 3h, 4h and count remaining viable count immediately, comparing with former viable count,
Result shows, Viable detection is 97%.Then it is taken in simulated gastric fluid that to digest the nutrient solution of different time each
1mL, is inoculated in the simulated intestinal fluid that 9mL pH value is 6.8 respectively, is placed in 30-45 DEG C of quiescent culture 2-4h,
And respectively 0,3,6,24h sampling, measure its viable count, compare with former viable count, result shows
Viable detection is 99%.
2, the resistance test of cholate is simulated: make the solution of 1g/L with pancreatin, and add 0.8% in the solution
Pig cholate, with 10% NaOH adjust pH be 8.0, then with 0.45 μm micro-filtrate membrane filtration also
Degerming.The probiotic solution kept by 0.5mL is inoculated in 4.5mL simulation cholate, after cultivating 24h
Obtain nutrient solution, the viable count of counting remaining.By nutrient solution, in sterile saline, ten times of stepwise dilutions are extremely
10-8, and pour into MRS, it is subsequently placed in 35 DEG C of quiescent culture 24h.
Result shows that Viable detection is 99%.
Above result of the test shows, probio in nutritional health food of the present invention is probiotic (resistance to pH, resistance to
Cholate) relatively strong, it is especially suitable for human intestines and stomach's environment, in simulated gastrointestinal environments, survival rate is big, can effectively change
Kind gastrointestinal function.
It should be understood that nutritional health food prepared by embodiment of the present invention 1-3 has above-mentioned experiment equally
Effect, between each embodiment and little with above-mentioned experiment effect otherness.
Lowering cholesterol effect is tested by embodiment 6 present invention
1. experiment material
1.1 animals used as test and feed
Kunming mouse, ♂, body weight (20 ± 2) g, Traditional Chinese Medicine University Of Guangzhou's Experimental Animal Center provide；
Normal diet is commercially available mouse feed；High lipid food is by for 60% normal diet+10% lard+10%
Milk powder+10% yolk powder+7% white sugar+2% peanut+0.5% salt+0.5% sesame oil；
1.2 reagent
T-CHOL kit (TCH, Eastern Europe, Zhejiang diagnostic products Co., Ltd)
Feed reagent to configure:
Sample liquid is 1.: 2g embodiment 3 product adds 50mL ultra-pure water, vibration mixing, to obtain final product；
Sample liquid is 2.: in embodiment 3 product preparation process, CGMCC NO.11763 fermentation medium removes courage
Sterol, the product 2g of the constant preparation of other conditions adds 50mL ultra-pure water, vibration mixing, to obtain final product
Comparison liquid: pure water；
2. method
The packet of 2.1 mouse and raising
After mouse adaptability is fed 5 days, it is randomly divided into 2 groups: blank group, experimental group, often group 20；
Feed 75 days；
Blank group: high lipid food, to pure water, volume is identical with sample liquid liquor capacity；
Experimental group is 1.: high lipid food, to sample liquid 1. 800mg/kg.d；
Experimental group is 2.: high lipid food, to sample liquid 2. 800mg/kg.d；After last is administered, water is can't help in fasting
12 hours, eye socket took blood and prepares serum, measured T-CHOL (TC) according to kit specification method；Experiment knot
Fruit is as follows:
| Group | Before experiment | 75 days |
| Blank group | 2.32±0.25 | 3.15±0.32 |
| Experimental group is 1. | 2.33±0.15 | 2.36±0.27 |
| Experimental group is 2. | 2.32±0.31 | 2.54±0.33 |
From experimental result, before and after experiment, experimental group and control group mice cholesterol in serum significant difference,
Product of the present invention has a significant effect to reducing serum cholesterol levels.
Embodiment 7 product of the present invention improves the effect experimental of immunity
This product embodiments 3 product selects 100 ages of Beijing area at 60-75 year weak easy catching a cold in the winter time
The elderly eat January continuously, eat 5 grams every day, with before edible and edible after effectiveness comparison, the results are shown in Table
1, result shows that raising the elderly's resistance, enhancing body immunity are had positive effect, product to have by this product
Good edible and nutritious tonifying effect.Simultaneously can significantly improve sleep quality, improvement rate reach 70% (with
Have quality length of one's sleep for assessment foundation) the most edible many this product old men reflect take for a long time better, annual
Having no interest, health is more preferable.
The table 6 eating effect table of comparisons
| Number | Edible first 30 days times of common cold (total) | Times of common cold (total) in 30 days after edible February |
| 100 | 31 | 6 |
Embodiment 8 lentinan extraction effect contrast test
Method 1: lentinan extracting method is as follows:
(1) take after commercially available mushroom fruiting body dries pulverizing and cross 80 mesh sieves；
(2) ultrasonic hot water extraction: add pure water in the ratio of mass volume ratio 3:20, under the conditions of pH7,
400W ultrasonic wave, 105 DEG C of water-bath extraction 0.5h；
(3) vapours extraction: said extracted system is placed in autoclave, 121 DEG C, 5min；
(4) circulation repeat step (2), (3) 4 times；
(5) centrifugation obtains supernatant；
(6) supernatant reduced pressure concentration obtains concentrate, adds 95% ethanol of 3 times of volumes of concentrate, at 4 DEG C
Alcohol analysis 24h, abandoning supernatant, after adding absolute ethyl alcohol isopyknic with initial concentration liquid standing 30min,
Abandoning supernatant, washing of precipitate 3 times, spray drying is weighed, and obtains lentinan.
Method 2: lentinan extracting method is as follows:
(1) take after commercially available mushroom fruiting body dries pulverizing and cross 80 mesh sieves；
(2) ultrasonic hot water extraction: add pure water in the ratio of 3:20, under the conditions of pH7,400W surpasses
Sound wave, 105 DEG C of water-bath extraction 140min；
(3) centrifugation obtains supernatant；
(4) supernatant reduced pressure concentration obtains concentrate, adds 95% ethanol of 3 times of volumes of concentrate, at 4 DEG C
Alcohol analysis 24h, abandoning supernatant, after adding absolute ethyl alcohol isopyknic with initial concentration liquid standing 30min,
Abandoning supernatant, washing of precipitate 3 times, spray drying is weighed, and obtains lentinan.
The thick recovery rate of lentinan (%)=(dry weight after Thick many candies dry weight/mushroom fruiting body drying) × 100
Calculate the impact of above two extracting method recovery rate thick on lentinan according to above-mentioned formula, method 1 is fragrant
The thick recovery rate of mushroom polysaccharide is 12.59%, and the thick recovery rate of method 1 lentinan is 10.31%, it is seen then that intermittent fever
Steam extraction can be effectively improved the ultrasonic hot water extraction thick recovery rate to lentinan.
Claims (10)
1. a compound probiotic fungi functional food, is made up of the raw material of following parts by weight: matsutake enzymolysis powder 20-70 part,
Ophiopogon japonicus extract 5-10 part, lentinan 5-10 part, FOS 2-10 part, Semen Tritici aestivi fiber 2-10 part, fibre and soya 2-5
Part, Tea Polyphenols 0.01-0.03 part, malt extract 10-50 part, Lactobacillus plantarum pulvis 20-40 part；
Described Lactobacillus plantarum pulvis is prepared by Lactobacillus plantarum (Lactobacillus plantarum) tlj-2015, and preserving number is
CGMCC NO.11763。
2. a kind of compound probiotic fungi functional food as claimed in claim 1, it is characterised in that described matsutake enzymolysis powder
Preparation method as follows:
(1) Tricholoma matsutake (lto et lmai) Singer sporophore drying and crushing；
(2) water-soluble homogeneous: the Tricholoma matsutake (lto et lmai) Singer sporophore after pulverizing adds in stainless steel cylinder, adds the water of fructification quality 3-6 times,
Soaking 3-5 hour, then by this Tricholoma matsutake (lto et lmai) Singer sporophore liquid by colloid mill, colloid mill operation condition is: adjust colloid mill stator with
The gap of rotor is 0.5-1 micron, and colloid mill flow is 0.4-1 ton hour；
(3) intensification enzymolysis: transfer to be heated in stainless steel enzymatic vessel by the Tricholoma matsutake (lto et lmai) Singer sporophore liquid through milling treatment of colloid
50-60 DEG C, adjust pH to 4.5-6.0, addition the cellulase of Tricholoma matsutake (lto et lmai) Singer sporophore weight 0.05-0.1%, 0.01-0.1% β-
Dextranase, the protease of 0.01-0.1%, insulation enzymolysis 0.5-1.5 hour, enzymolysis process is stirred continuously；
(4) it is dried: be dried after the mash filtrations after enzymolysis and obtain matsutake enzymolysis powder；
Described ophiopogon japonicus extract preparation method is as follows:
The tuber of dwarf lilyturf pulverizes, and after crossing 40-50 mesh sieve, adds 3-6 times of weight absolute ethyl alcohol Soakage extraction tuber of dwarf lilyturf, controls temperature 30-45 DEG C,
After 2-4 hour adjust temperature be 55-60 DEG C 1-2 hour, extract concentrate, be dried to obtain ethanol extract；After alcohol extract
The tuber of dwarf lilyturf residue adds 75-85 DEG C of hot water, hot water addition is tuber of dwarf lilyturf 2-4 times of residue weight, and process time 30-50 divides
Clock, extracts 2-3 time continuously, is spray-dried, obtains hot water extract after being concentrated in vacuo by extract；By above-mentioned ethanol extract
Merge pulverizing with hot water extract, cross 60 mesh sieves, obtain ophiopogon japonicus extract.
3. a kind of compound probiotic fungi functional food as claimed in claim 1, it is characterised in that described lentinan carries
Access method is as follows:
(1) take after commercially available mushroom fruiting body dries pulverizing and cross 80 mesh sieves；
(2) ultrasonic hot water extraction: add pure water in the ratio of the mushroom fruiting body after sieving with water quality volume ratio 1-3:20,
Under the conditions of pH7,400W ultrasonic wave, 105 DEG C of water-bath extraction 0.5h；
(3) vapours extraction: said extracted system is placed in autoclave, 121 DEG C, 5min；
(4) circulation repeat step (2), (3) 4 times；
(5) centrifugation obtains supernatant；
(6) supernatant reduced pressure concentration obtains concentrate, adds 95% ethanol of 3 times of volumes of concentrate, and at 4 DEG C, alcohol analysis 24h, abandons
Remove supernatant, after adding absolute ethyl alcohol isopyknic with initial concentration liquid standing 30min, abandoning supernatant, washing of precipitate 3
Secondary, it is spray-dried, obtains lentinan.
4. a kind of compound probiotic fungi functional food as claimed in claim 1, it is characterised in that described Lactobacillus plantarum
In pulvis, viable bacteria content is: 7x1012-9x1012cfu/g。
5. described a kind of compound probiotic fungi functional food as claimed in claim 1, it is characterised in that described plant
The preparation method of lactobacillus pulvis is as follows:
(1) take Lactobacillus plantarum CGMCC NO.11763 mono-ring on inclined-plane, access and cultivate equipped with 50mL culture medium MRS
In the 250mL triangular flask of base, 200rpm, cultivates 12-16h, makes thalline be in mid log phase for 37 DEG C；
(2) accessing in fermentation medium by the bacterial classification of logarithmic phase, inoculum concentration is 100-200rpm training at 10-15%, 35-38 DEG C
Supporting 8-12 hour, dissolved oxygen controls 10%, Anaerobic culturel 60-70 hour the most again；The complete zymotic fluid that ferments staticly settles, is centrifuged
Being precipitated thing, then add the carrier of sediment quality 1 times, mix, 50 DEG C of fluidized bed dryings i.e. obtain Lactobacillus plantarum
Pulvis, vehicle weight number consists of: sour milk powder 25 parts, 12 parts of dextrin.
6. a kind of compound probiotic fungi functional food as claimed in claim 5, it is characterised in that described fermentation medium
Form as follows: peptone 10-12g, beef extract 10-12g, yeast extract 5-8g, diammonium hydrogen citrate 2-4g, glucose
20-25g, Tween 80 1-2mL, sodium acetate 5-7g, dipotassium hydrogen phosphate 2-3g, magnesium sulfate 0.58-0.6g, manganese sulfate 0.25-0.3
G, cholesterol 100-120mg, Chinese herbal medicine powder 5-8g, distilled water 1 000mL, pH 6.2～6.6.
7. a kind of compound probiotic fungi functional food as claimed in claim 6, it is characterised in that adding of described cholesterol
Adding method is: first use anhydrous alcohol solution cholesterol, and compound concentration is the cholesterol solution of 10-12mg/mL, then by necessarily than
Example is added in culture medium, and making cholesterol ultimate density is 100-120 μ g/mL.
8. a kind of compound probiotic fungi functional food as claimed in claim 6, it is characterised in that described Chinese herbal medicine powder
Preparation method as follows:
Weigh fruit of Chinese magnoliavine 10-20 part；Radix Codonopsis 10-15 part；Hawthorn 10-20 part；It is 2 that said herbal medicine is crushed to particle diameter respectively
Below Hao meter, in container, then uniformly mix and add the water of 3-6 times of weight, control temperature 45-60 DEG C and keep 2～4h, add
Entering mixing enzyme preparation and carry out enzymolysis, regulation pH value is 6-7, enzymolysis 2-4h, finally adds 0.5-3 times of w ethanol of mixed material
With the mixture of propyl alcohol, ultrasonic extraction 0.5～1.5h under 110W power, filter；Filter vacuum concentrates in postlyophilization acquisition
Herbal medicine pulvis；
Described mixed enzyme addition is the 5-10% of mixed material gross weight；
The parts by weight of described mixed enzyme consist of: zytase 15-20 part, beta amylase 10-15 part, pectase 10-15 part,
Acid protease 10-15 part, acid phosphatase 5-10 part；
The mass ratio of described ethanol and propyl alcohol is 1:1-2.
9. described a kind of compound probiotic fungi functional food as claimed in claim 1, it is characterised in that by following heavy
The raw material composition of amount number: 50 parts of matsutake enzymolysis powder, ophiopogon japonicus extract 8 parts, mushroom lentinan 8 parts, FOS 7 parts,
Semen Tritici aestivi fiber 7 parts, fibre and soya 3 parts, Tea Polyphenols 0.02 part, malt extract 30 parts, 30 parts of Lactobacillus plantarum pulvis.
10. compound probiotic fungi functional food described in claim 1 is in the application of healthcare field.
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| WO2019010813A1 (en) * | 2017-07-13 | 2019-01-17 | 吴炳新 | Microbial fermentation composition and use thereof |
| CN111903982A (en) * | 2020-07-16 | 2020-11-10 | 奥克斯路株式会社 | Bifidobacterium BZ25 fermented milk with cholesterol lowering effect |
| CN114651985A (en) * | 2022-04-12 | 2022-06-24 | 广州市沐家健康产业有限公司 | Probiotic powder with weight-losing effect and preparation method thereof |
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| CN111903982A (en) * | 2020-07-16 | 2020-11-10 | 奥克斯路株式会社 | Bifidobacterium BZ25 fermented milk with cholesterol lowering effect |
| JP2022024959A (en) * | 2020-07-16 | 2022-02-09 | オクスロ株式会社 | Bifidobacteria bz25 fermented milk having cholesterol reducing effect |
| JP7090144B2 (en) | 2020-07-16 | 2022-06-23 | オクスロ株式会社 | Bifidobacterium BZ25 fermented milk with cholesterol lowering effect |
| CN114651985A (en) * | 2022-04-12 | 2022-06-24 | 广州市沐家健康产业有限公司 | Probiotic powder with weight-losing effect and preparation method thereof |
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