CN105850518A - Method for increasing grafting propagation survival rate of pinus bungeana variations - Google Patents
Method for increasing grafting propagation survival rate of pinus bungeana variations Download PDFInfo
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- CN105850518A CN105850518A CN201610244418.6A CN201610244418A CN105850518A CN 105850518 A CN105850518 A CN 105850518A CN 201610244418 A CN201610244418 A CN 201610244418A CN 105850518 A CN105850518 A CN 105850518A
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- scion
- grafting
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- pine
- lacebark pine
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/30—Grafting
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- Life Sciences & Earth Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a method for increasing a grafting propagation survival rate of pinus bungeana variations. The method includes: grafting a scion to a rootstock according to a high-position inarching method to complete grafting and obtain a grafted plant, wherein the scion refers to a pinus bungeana variation or pinus bungeana, the pinus bungeana variation refers to Penxian pinus bungeana, and the rootstock refers to pinus tabuliformis or pinus bungeana. A process of grafting the scion to the rootstock according to the high-position inarching method includes: cutting the scion to obtain the scion with a cut, and cutting the rootstock at a position 2-5cm below a terminal bud of an upper branch or a main branch of the rootstock to obtain the rootstock with a cut; jointing the scion with the cut and the rootstock with the cut to finish grafting. The survival rate of pinus bungeana grafted according to the method is about 90% generally, and high affinity and quickness in growth are realized. In addition, the method can be applied to breeding of new pinus bungeana varieties, establishment of pinus bungeana genetic resource clone collection nurseries and breeding of pinus bungeana clones in production.
Description
Technical field
The present invention relates to a kind of method improving lacebark pine variation type propagation by grafiting survival rate.
Background technology
Lacebark pine is the unique three pin pines in North China of China, and its tree crown is the most colourful, and trunk is tall and straight dark green, bark spot
Refute such as dragon, be commonly called as " emperor tree ", be the garden landscape seeds that northern China is the most excellent.But it is raw during lacebark pine seedling
Long slow (1 year high 3-5cm of life, 10 years high about 1m of life), limits it and quickly becomes a useful person and develop.Particularly, closely
Over Nian, scientific research personnel is found that strain lacebark pine variation type--a Spur Type lacebark pine, named " basin celestial being lacebark pine "." basin
Celestial lacebark pine " observe through more than ten years, have no up till now and blossom and bear fruit, the most only by asexual method, it is carried out
Breeding is promoted.Lacebark pine asexual reproduction method is presently mainly by the grafting of this anvil or heteroplastic grafting, and the grafting of this anvil is with white
Pi Songmiao or tree do stock, and heteroplastic grafting generally does stock with Chinese pine, black pine seedling or tree, and grafting time is generally in summer
Hot and humid weather is carried out.It is relatively low, to sky during grafting that the problem that common engrafting method exists is usually graft survival rate
Gas and stock require height, and grafting procedures needs to complete for 2 years (grafting upper one year, next year lighten restrictions on).
Summary of the invention
The technical problem to be solved is how to improve lacebark pine variation type propagation by grafiting survival rate.
For solving above-mentioned technical problem, present invention firstly provides lacebark pine engrafting method, described method includes: use
High-order inarching method, by scion grafting to stock, completes grafting, obtains grafting plant；Described scion is variation lacebark pine
Or lacebark pine.
In said method, described variation lacebark pine can be basin celestial being lacebark pine；And/or, described stock can be Chinese pine or white skin
Pine.
In said method, described scion is cut out by the high-order inarching method of described employing by comprising the steps that in scion grafting to stock
Otch, obtains the scion with otch, cuts out at 2cm-5cm below master's slightly terminal bud of described stock or branch slightly terminal bud
Otch, obtains the stock with otch；The described scion with otch of docking and the described stock with otch, complete
Grafting.
Wherein, the described scion with otch and the described stock with otch form layer tight connecting and form grafting part.
Stock described in every strain can be high position grafting 1-4 described scion, and the described unnecessary branch of stock low level is wiped out in grafting after completing.
In said method, described stock can be lacebark pine or Chinese pine in seedling stage in seedling stage.The height of described stock can be more than 1m.
In said method, described scion can be 1 year raw young sprout of lacebark pine or basin celestial being lacebark pine.Described stock can be with
Female stem terminal bud.
In said method, within 2-5 days before grafting, can be that described stock waters to ensure that moisture is sufficient.
In said method, can be by described scion moisturizing before grafting.The method of described moisturizing is concretely by described scion
Steep after collection in water, during grafting, water is outwelled, described scion covers wet towel, is drawn off during grafting.
In said method, the length of described scion can be 3-5 centimetre.
In said method, the diameter of described scion can be 0.3-0.5 centimetre.
Said method can be 5-30 DEG C in environment temperature, envionmental humidity is to carry out in 60-80%.Described environment temperature
Spend concretely 15-25 DEG C.
In said method, can to keep ambient humidity after grafting be 40%-60%, environment temperature is 5 DEG C-30 DEG C.
Said method may also include that transplants described grafting plant to the environment of 25 DEG C-35 DEG C, obtains purpose plant.
In said method, after being transplanted to the environment of 25 DEG C-35 DEG C by described grafting plant, may also include the described institute of removing
State female stem terminal bud of stock described in grafting plant.
In said method, to all can be controlled big of temperature, humidity before described transplanting after described grafting and described grafting
Canopy is carried out.
Can carry out in booth during the method grafting that the present invention provides, be not required to rely on weather condition, stock scope also can increase
Width, grafting procedures completes (grafting then, lighten restrictions on then) for 1 year, utilizes the method grafting lacebark pine one that the present invention provides
As survival rate all reach about 90%, and affinity is strong, and growth is fast.
Advantages of the present invention is as follows:
(1) be conducive to Fast-propagation lacebark pine choiceness and new varieties, keep the stability of its kind with consistent
Property.
(2) be conducive to improving the early growth speed of lacebark pine nursery stock.
(3) be conducive to downgrading lacebark pine, be used for making potted landscape.
(4) the method has repeated to test more than 3 years, effect stability.
The method that the present invention provides can apply to breed lacebark pine new varieties, set up lacebark pine germ plasm resource collect garden with
And on producing the lacebark pine clone of breed good.
Accompanying drawing explanation
Fig. 1 is basin celestial being lacebark pine complete stool after grafting.
Fig. 2 is basin celestial being lacebark pine.
Fig. 3 is the basin celestial being lacebark pine ball after grafting.
Detailed description of the invention
Below in conjunction with detailed description of the invention, the present invention is further described in detail, the embodiment be given only for
Illustrate the present invention rather than in order to limit the scope of the present invention.
Experimental technique in following embodiment, if no special instructions, is conventional method.
Material used in following embodiment, reagent etc., if no special instructions, the most commercially obtain.
Lacebark pine (Pinus bungeana) in following embodiment document " He Yan, Li Bin, Jiang Yingshu etc., in vain
The variation of skin pine Species/Provenance and selection, forestry science and technology communication, 2001,6:5-8.Mistake disclosed in ", the public can be from application
Obtain at people.
Basin celestial being lacebark pine in following embodiment, at document, " Li Bin, Meng Qingyang, Li Yanda etc., lacebark pine kind matter provides
Source identify with evaluate, Hunan Forestry science and technology, 2016,43 (2): 1-7. " disclosed in mistake, the public can obtain at applicant
?.
Embodiment 1, the method for grafting lacebark pine
One, grafting time: spring (February-March), outdoor temperature-20 DEG C to 15 DEG C, canopy temperature 5 DEG C to 30 DEG C.
Graft stock: high more than 1m lacebark pine or the seedlings of Chinese pine or little tree.
Grafting scion: 1 year raw basin celestial being lacebark pine and the spray of lacebark pine individuality, diameter is about 0.3-0.5 centimetre.
Two, engrafting method
(1) carrying out in booth at mid-February-late March, the minimum temperature in booth is maintained at more than 5 DEG C,
It is advisable with 15-25 DEG C.
(2) graft stock is transplanted in booth, within before grafting 2-5 days, water to rootstock seedling the most permeable to ensure water
Divide abundance.
(3) before grafting 1-2 days, give the graft stock planted booth garden water a water, grafting same day, give
Booth surrounding is sprayed water repeatedly, increases air humidity, and relative humidity controls to be preferred at 60-80%.
(4) the grafting same day, the healthy and strong young sprout (i.e. spray) of 1 year raw basin celestial being lacebark pine of clip or conventional lacebark pine is made
Scion, length 3-5cm, with adopting with steeping in water, during grafting, water is outwelled, cover wet towel, with connecing with taking.
(5) grafting uses high-order inarching method (high connection), first scion is cut half, then cuts stock, at anvil
Cut notch at 2-5cm below main slightly terminal bud or the branch slightly terminal bud of wood, scion cut alignment stock otch be inserted into placement,
Both are made to form a layer tight connecting.Grafting part film stretches tight and twines, and retains female stem terminal bud during grafting.Every strain stock is the most excellent
First 1-4 scion of high position grafting (can be more), the unnecessary branch of stock low level be wiped out in grafting after completing.
(6) after booth grafts, in 10-30 days, if temperature of shed is more than 30 DEG C, need to shade,
Weather turn warm after to be aerated, keep environment temperature at 5 DEG C-30 DEG C, ambient humidity is at 40%-60%.
(7) sapling of grafting survival can be removed booth by the 7-8 month (outdoor temperature 25 DEG C-35 DEG C), transplants land for growing field crops；
After transplanting, the film twined that can grafting part be stretched tight is removed, and female stem terminal bud will be stayed to cut, it is simple to scion grows.
Use the basin celestial being lacebark pine of above method grafting survival as shown in Figure 1-Figure 3.
The Relationship between Graft Effect of the method: general survival rate is up to 90%, and affinity is strong, and growth is fast.
Graft survival rate is added up: 2009 to 2013 years, and scion is respectively adopted basin celestial being lacebark pine and conventional lacebark pine,
Stock is respectively adopted conventional lacebark pine and Chinese pine, and continuous 4 years repeat.Utilize this method basin celestial being lacebark pine grafting conventional white
Skin pine 4 annual graft survival rates are 91.2%, and the basin celestial being lacebark pine average graft survival rate of grafting Chinese pine is 88.5%, often
The average graft survival rate of rule lacebark pine scion grafting routine lacebark pine and Chinese pine is respectively 90.3% and 86.9%, equal and basin
Celestial lacebark pine scion graft survival rate is close, and the graft survival rate timing statistics of the present invention is November then.
Claims (10)
1. lacebark pine engrafting method, described method includes: uses high-order inarching method by scion grafting to stock, completes
Grafting, obtains grafting plant；Described scion is variation lacebark pine or lacebark pine.
Method the most according to claim 1, it is characterised in that: described variation lacebark pine is basin celestial being lacebark pine；With
/ or, described stock is Chinese pine or lacebark pine.
Method the most according to claim 1 and 2, it is characterised in that: scion is transferred by the high-order inarching method of described employing
It is connected on stock include: described scion is cut notch, obtains the scion with otch, the master of described stock is slightly pushed up
Cut notch at 2cm-5cm below bud or branch slightly terminal bud, obtain the stock with otch；Docking is described with otch
Scion and the described stock with otch, complete grafting.
4. according to the arbitrary described method of claim 1-3, it is characterised in that: described stock is the big of lacebark pine or Chinese pine
Seedling or little tree.
5. according to the arbitrary described method of claim 1-4, it is characterised in that: described scion be lacebark pine or basin celestial white
The raw young sprout in 1 year of Pi Song；
And/or, described stock is with female stem terminal bud.
6. according to the arbitrary described method of claim 1-5, it is characterised in that: a length of 3-5 centimetre of described scion；
And/or, a diameter of 0.3-0.5 centimetre of described scion.
7. according to described method arbitrary in claim 1-6, it is characterised in that: described method in environment temperature is
5-30 DEG C, envionmental humidity be that 60-80% is carried out.
8. according to the arbitrary described method of claim 1-7, it is characterised in that: described environment temperature is 15-25 DEG C.
9. according to the arbitrary described method of claim 1-8, it is characterised in that: described method also includes: transfer described
Connect in the environment of plantlet of transplant to 25 DEG C-35 DEG C, obtain purpose plant.
Method the most according to claim 9, it is characterised in that: described grafting plant is transplanted to 25 DEG C-35 DEG C
Environment in after also include female stem terminal bud of removing stock described in described grafting plant.
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| CN201610244418.6A CN105850518A (en) | 2016-04-19 | 2016-04-19 | Method for increasing grafting propagation survival rate of pinus bungeana variations |
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| CN201610244418.6A CN105850518A (en) | 2016-04-19 | 2016-04-19 | Method for increasing grafting propagation survival rate of pinus bungeana variations |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112226433A (en) * | 2020-07-31 | 2021-01-15 | 中国林业科学研究院林业研究所 | SNP (Single nucleotide polymorphism) site primer combination for identifying white bark pine germplasm resources and application |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1994052A (en) * | 2006-01-05 | 2007-07-11 | 尹世亮 | Seedling raising process using black pine to engraft white bark pine |
| CN103814759A (en) * | 2014-02-22 | 2014-05-28 | 西安市华正农业科技发展有限公司 | Seedling growing technology for grafting white bark pine on Chinese pine |
-
2016
- 2016-04-19 CN CN201610244418.6A patent/CN105850518A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1994052A (en) * | 2006-01-05 | 2007-07-11 | 尹世亮 | Seedling raising process using black pine to engraft white bark pine |
| CN103814759A (en) * | 2014-02-22 | 2014-05-28 | 西安市华正农业科技发展有限公司 | Seedling growing technology for grafting white bark pine on Chinese pine |
Non-Patent Citations (4)
| Title |
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| 上海市园林管理处编: "《城市绿化植树》", 31 December 1976, 中国建筑工业出版社 * |
| 李润龙等: "《白皮松繁育栽植技术》", 《陕西林业科技》 * |
| 李靖编著: "《鲜食桃》", 30 October 2006, 河南科学技术出版社 * |
| 辽宁省农林局编: "《辽宁果树技术手册》", 31 January 1977, 辽宁人民出版社 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112226433A (en) * | 2020-07-31 | 2021-01-15 | 中国林业科学研究院林业研究所 | SNP (Single nucleotide polymorphism) site primer combination for identifying white bark pine germplasm resources and application |
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Application publication date: 20160817 |