CN105838606A - 一种高通量细胞联合培养芯片 - Google Patents
一种高通量细胞联合培养芯片 Download PDFInfo
- Publication number
- CN105838606A CN105838606A CN201610400086.6A CN201610400086A CN105838606A CN 105838606 A CN105838606 A CN 105838606A CN 201610400086 A CN201610400086 A CN 201610400086A CN 105838606 A CN105838606 A CN 105838606A
- Authority
- CN
- China
- Prior art keywords
- culture
- cell
- platforms
- groups
- cell culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000003501 co-culture Methods 0.000 title claims description 13
- 238000004113 cell culture Methods 0.000 claims abstract description 67
- 230000010412 perfusion Effects 0.000 claims abstract description 53
- 235000015097 nutrients Nutrition 0.000 claims abstract description 31
- 239000003364 biologic glue Substances 0.000 claims abstract description 10
- 239000007788 liquid Substances 0.000 claims description 53
- 239000003292 glue Substances 0.000 claims description 19
- 108010027529 Bio-glue Proteins 0.000 claims description 16
- 239000000084 colloidal system Substances 0.000 claims description 16
- 239000010410 layer Substances 0.000 claims 2
- 239000011229 interlayer Substances 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 56
- 239000000243 solution Substances 0.000 description 26
- 108010082117 matrigel Proteins 0.000 description 10
- 238000010586 diagram Methods 0.000 description 9
- 239000011257 shell material Substances 0.000 description 8
- 230000003993 interaction Effects 0.000 description 5
- 229940044683 chemotherapy drug Drugs 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000004088 simulation Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 102000004127 Cytokines Human genes 0.000 description 2
- 108090000695 Cytokines Proteins 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 239000004205 dimethyl polysiloxane Substances 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000019522 cellular metabolic process Effects 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000007877 drug screening Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003102 growth factor Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000008611 intercellular interaction Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000005445 natural material Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- -1 polydimethylsiloxane Polymers 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 150000003384 small molecules Chemical class 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000009967 tasteless effect Effects 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Clinical Laboratory Science (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Dispersion Chemistry (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Description
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610400086.6A CN105838606B (zh) | 2016-06-08 | 2016-06-08 | 一种高通量细胞联合培养芯片 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610400086.6A CN105838606B (zh) | 2016-06-08 | 2016-06-08 | 一种高通量细胞联合培养芯片 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN105838606A true CN105838606A (zh) | 2016-08-10 |
CN105838606B CN105838606B (zh) | 2018-10-02 |
Family
ID=56575918
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201610400086.6A Active CN105838606B (zh) | 2016-06-08 | 2016-06-08 | 一种高通量细胞联合培养芯片 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN105838606B (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108949562A (zh) * | 2018-09-08 | 2018-12-07 | 重庆科技学院 | 一种细胞毒性实验的微流控芯片 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103087912A (zh) * | 2011-10-27 | 2013-05-08 | 中国科学院大连化学物理研究所 | 一种产生稳定浓度梯度的微流控芯片及细胞共培养方法 |
CN103103121A (zh) * | 2013-01-17 | 2013-05-15 | 中国科学院深圳先进技术研究院 | 细胞培养微流控芯片 |
US8679843B2 (en) * | 2009-10-12 | 2014-03-25 | Corning Incorporated | Microfluidic device for cell culture |
CN104388310A (zh) * | 2014-10-31 | 2015-03-04 | 青岛大学附属医院 | 一种细胞联合培养芯片 |
-
2016
- 2016-06-08 CN CN201610400086.6A patent/CN105838606B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US8679843B2 (en) * | 2009-10-12 | 2014-03-25 | Corning Incorporated | Microfluidic device for cell culture |
CN103087912A (zh) * | 2011-10-27 | 2013-05-08 | 中国科学院大连化学物理研究所 | 一种产生稳定浓度梯度的微流控芯片及细胞共培养方法 |
CN103103121A (zh) * | 2013-01-17 | 2013-05-15 | 中国科学院深圳先进技术研究院 | 细胞培养微流控芯片 |
CN104388310A (zh) * | 2014-10-31 | 2015-03-04 | 青岛大学附属医院 | 一种细胞联合培养芯片 |
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108949562A (zh) * | 2018-09-08 | 2018-12-07 | 重庆科技学院 | 一种细胞毒性实验的微流控芯片 |
CN108949562B (zh) * | 2018-09-08 | 2023-09-22 | 重庆科技学院 | 一种细胞毒性实验的微流控芯片 |
Also Published As
Publication number | Publication date |
---|---|
CN105838606B (zh) | 2018-10-02 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
Zheng et al. | Organ‐on‐a‐chip systems: microengineering to biomimic living systems | |
Ghaemmaghami et al. | Biomimetic tissues on a chip for drug discovery | |
CN104328040B (zh) | 一种肿瘤化疗药物敏感性检测芯片及使用方法 | |
Gao et al. | Recent developments in microfluidic devices for in vitro cell culture for cell-biology research | |
CN105950469B (zh) | 细胞筛选芯片及微流控联合芯片 | |
CN111218404A (zh) | 一种仿生多器官芯片及其制备方法和应用 | |
CN105176816B (zh) | 一种基于细胞聚集体的微脉管肝脏芯片及其制备和使用方法 | |
CN103952300B (zh) | 一种微流控芯片及细胞趋化运动研究方法 | |
CN105080627A (zh) | 一种用于药物筛选的集成化微流控芯片及其应用方法 | |
CN103087912A (zh) | 一种产生稳定浓度梯度的微流控芯片及细胞共培养方法 | |
CN108117990B (zh) | 一种基于微流控技术的仿生气血屏障模型的构建方法 | |
CN102021116A (zh) | 一种微流控芯片及其研究非接触式细胞共培养的方法 | |
CN112300940A (zh) | 一种基于微流控技术构建的牙周软组织仿生芯片及其应用 | |
Liu et al. | Advances in microfluidic technologies in organoid research | |
CN112852627A (zh) | 一种基于多器官芯片的多能干细胞来源人肝和胰岛共培养的方法 | |
CN113278525B (zh) | 一种干细胞球或肿瘤球培养装置及培养方法 | |
CN110551679A (zh) | 一种含腺泡三脉管结构肝单元芯片的精准打印构建方法 | |
CN204644371U (zh) | 一种集成式多细胞非接触共培养装置 | |
CN116333881A (zh) | 一种三通道器官芯片及其应用和使用方法 | |
Deng et al. | Organ-on-a-chip: future of female reproductive pathophysiological models | |
CN104388310B (zh) | 一种细胞联合培养芯片 | |
Guo et al. | A generic pump‐free organ‐on‐a‐chip platform for assessment of intestinal drug absorption | |
CN101451105A (zh) | 一种毛细血管模型的构建方法及其微系统芯片 | |
CN105838606B (zh) | 一种高通量细胞联合培养芯片 | |
CN114317272B (zh) | 一种多细胞共培养的培养装置及细胞培养方法 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant | ||
CB03 | Change of inventor or designer information | ||
CB03 | Change of inventor or designer information |
Inventor after: Niu Haitao Inventor after: Wang Jianning Inventor after: Wang Yunchao Inventor before: Niu Haitao Inventor before: Wang Yunchao Inventor before: Wang Jianning |
|
TR01 | Transfer of patent right | ||
TR01 | Transfer of patent right |
Effective date of registration: 20230719 Address after: No. 16, Jiangsu Road, Southern District, Shandong, Qingdao, Shandong Patentee after: THE AFFILIATED HOSPITAL OF QINGDAO University Address before: No. 16, Jiangsu Road, Southern District, Shandong, Qingdao, Shandong Patentee before: Niu Haitao |