CN105819912A - Production method of ecological bacterial fertilizer rich in polysaccharide, phosphate solubilizing bacteria, and potassium solubilizing bacteria - Google Patents
Production method of ecological bacterial fertilizer rich in polysaccharide, phosphate solubilizing bacteria, and potassium solubilizing bacteria Download PDFInfo
- Publication number
- CN105819912A CN105819912A CN201610144697.9A CN201610144697A CN105819912A CN 105819912 A CN105819912 A CN 105819912A CN 201610144697 A CN201610144697 A CN 201610144697A CN 105819912 A CN105819912 A CN 105819912A
- Authority
- CN
- China
- Prior art keywords
- seed
- liquid
- production method
- culture
- bacterial manure
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F11/00—Other organic fertilisers
- C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
Abstract
The invention discloses a production method of an ecological bacterial fertilizer rich in polysaccharide, phosphate solubilizing bacteria, and potassium solubilizing bacteria. The production method comprises the following steps: (1) culturing trichoderma seeds; (2) performing fermentation culture; (3) carrying out enzymatic hydrolysis and extraction. Due to the enzymatic hydrolysis in the previous step, water contains a large amount of carbon sources and nitrogen sources that can be utilized; the dreg-containing hydrolysis liquid is extruded to obtain solids, the solids are dried and saved for later use; the liquid is modulated, the pH of the liquid is adjusted, then the liquid is introduced into a fermentation tank, bacillus mucilaginosus, bacillus subtilis or bacillus pumilus is inoculated to the liquid to carry out fermentation, then liquid is filtered to obtain bacterium bodies, the bacterium bodies are added into the dried solids, and then the mixture is blended and dried in the air (or dried at a low temperature) to obtain the ecological bacterial fertilizer.
Description
Technical field
The invention belongs to ecological bacterial manure production technical field, be specifically related to a kind of rich in polysaccharide, rich in the production method of ecological bacterial manure of dissolving phosphor and dissolving potassium.
Background technology
Waste residue, refers to the solid gunk abandoned in human being's production, life process, and it is the product of human material's civilization.Substantial amounts of solid waste enters environment, do not only take up a large amount of soil, and, heavy metal and virus, pathogenic bacteria, the source of disease bodies such as mercurous, chromium, lead, nickel, copper, aluminum, stannum, vanadium, molybdenum, ferrum, the manganese that contain in solid waste, pollute surrounding, destroy ecological balance, bring serious health hazard to the mankind.
Waste residue resource utilizes, it is simply that waste residue is considered as secondary resource, makes them recycle as raw material.At present, existing many countries, by economic lever and mandatory administration means, encourage and support the development and application of reclamation of solid wastes technology.
The Chinese patent of such as Publication No. CN102249198A discloses a kind of method that sodium thiosulfate and sodium iodide are prepared in akali sulphide waste residue hydrolysis, mainly akali sulphide waste residue is first fully hydrolyzed, react with calcium sulfide again, then the filtrate after filtering is reacted with sodium sulfate and iodine simultaneously, reactant filters gained filtrate after going the removal of impurity and carries out decompression distillation, then carries out cooling with gradient cooling device and respectively obtains sodium thiosulfate and sodium iodide；The method as clean as a penny can gulp down waste residue, alleviates the waste residue pollution to environment；Valuable sodium thiosulfate and sodium iodide can be prepared again, not only there is significant Social benefit and economic benefit, and technique and the equipment of production are simple, be the actual process of a kind of comparison and Application way.
Although the method has its advantage, but the method for this hydrolysis waste residue, containing substantial amounts of acid ingredient or salt, it is impossible to carry out recycling, even can not be backfilling into field, become a big obstacle of industry sustainable development.
Summary of the invention
Goal of the invention: for above-mentioned weak point of the prior art, the invention provides a kind of rich in polysaccharide, rich in the production method of ecological bacterial manure of dissolving phosphor and dissolving potassium.
Technical scheme: the technical scheme that the invention provides, comprises the steps: 1) Trichoderma spp. seed culture: include that inclined-plane, shaking flask, first order seed, secondary seed are cultivated.2) fermentation culture: be initially charged bottom water in retort, is separately added into raw material, adjuvant in retort and is slowly stirred, and supplements water, sterilizing after mix homogeneously；Cooling, starts inoculation, accesses secondary seed, stir culture 48h-60h at 25 DEG C-30 DEG C, is passed through pure air；Described raw material is the dreg after the citric acid fermentation using Semen Maydis to be raw material, is crushed to 20-40 mesh.3) enzymolysis and extraction: cultivate after terminating, adds water, regulates pH, heats up, and is stirred enzymolysis, maintains temperature；After enzymolysis terminates, filter and remove liquid, and add the washing of a small amount of water, waste liquid and washings merging, carry out recycling treatment.
Concrete, each step is as follows:
1) Trichoderma spp. seed culture
Cultivate including inclined-plane, shaking flask, first order seed, secondary seed；
2) fermentation culture
Retort is initially charged bottom water 1000L, raw material, adjuvant are separately added in retort and are slowly stirred, mend and be filled with water to about 1500L, 115 DEG C of sterilizing 20min after mix homogeneously；It is cooled to 25 DEG C and starts inoculation, access secondary seed 10%, stir culture 48h-60h at 25 DEG C-30 DEG C, be passed through pure air 1.0M3/min；Described raw material is the dreg after the citric acid fermentation using Semen Maydis to be raw material, and 300kg is crushed to 20-40 mesh；
3) enzymolysis and extraction
After cultivation terminates, mend and add water to 2000L, regulate pH7.2, be warming up to 50 DEG C and be stirred enzymolysis, maintain temperature 4h；After enzymolysis terminates, filter and remove liquid, and add the washing of a small amount of water.Waste liquid and washings merge, and carry out recycling treatment.
Concrete, step 1) described in inclined-plane include:
Culture medium: Rhizoma Solani tuber osi 20%；Every 1000ml water adds fresh Rhizoma Solani tuber osi 200g, gauze filter and remove residue after boiling 20-30 minute；Glucose 20g/L, ammonium sulfate 5g/L, agar 2%；PH is natural, at sterilizing 121 DEG C 20 minutes；Slant culture temperature 25～30 DEG C；Inclined-plane seed can be placed in preservation 2 months at 0-4 DEG C of refrigerator, is forwarded to Shake flask medium during use.
Concrete, step 1) described in shaking flask include:
The same inclined-plane of Shake flask medium, but it is not added with agar.Shaking flask uses 500ml shaking flask, fills liquid 50-100ml；Shaking flask temperature 30 DEG C, shaking flask rotating speed 100～150rpm.
Concrete, step 1) described in first order seed include:
First order seed uses 20L fermentation tank, liquid amount 70%；Seed culture medium: Testa Tritici 15g/L, Semen Maydis powder 15g/L, ammonium sulfate 5g/L, KH2PO42g/L, regulates pH6.5-7.0；By seed culture medium at 115-125 DEG C of sterilizing 15-30min, inoculating trichoderma kind after being cooled to 30 DEG C, inoculum concentration is 10%, cultivates 36-48h, prepares primary seed solution for 30 DEG C.
Concrete, step 1) described in secondary seed include that secondary seed uses 500L, liquid amount 70%；Culture medium and the same first order seed of condition of culture；Incubation time 24-36h.
Concrete, step 2) described in adjuvant be Semen Maydis powder 10kg, ammonium sulphate 5kg, Semen sojae atricolor powder 2kg, KH2PO42kg。
Concrete, step 2) in fermentation culture carry out on 2500L multifunctional reaction tank.
In the present invention, bacterial manure makes due to the enzymatic hydrolysis of preceding processes, water liquid is available for utilizing containing a large amount of carbon sources, nitrogen source, band slag hydrolyzed solution is through extruding, solid content dried for standby, liquid enters fermentation tank after composition allotment and pH are allocated, and inoculation colloid or hay, the bacillus cereus such as short and small ferment, and filter and obtain thalline after terminating, add in the most dried solid material, dry after spice or cold drying obtains ecological bacterial manure.
Beneficial effect: the invention have the advantages that 1, is had benefited from the isolation technics of advanced person, is added by the ecological microbial agent rich in polysaccharide, rich in dissolving phosphor and dissolving potassium simultaneously, improve the value of waste residue resource.
2, it is organic that by-product ecology bacterial manure can either improve abundant carbon nitrogen, and its colloid bacillus cereus contained, there is the mineralization of uniqueness, it is possible to promote inorganic potassium, the release of Phos in soil, be effectively increased soil fertility.
3, the polysaccharide secreted by colloid bacillus cereus, additionally aid the improvement of soil microenvironment, improve Genes For Plant Tolerance arid and the ability of opposing pest and disease damage.
4, by the exploitation of the resource of waste residue, make the comprehensive benefit of industry obtain and significantly promote.
Detailed description of the invention:
Embodiment 1
1, Trichoderma spp. seed culture
Cultivate according to inclined-plane, shaking flask, first order seed, secondary seed.Culture process is as follows:
(1) inclined-plane:
Culture medium: (every 1000ml water adds fresh Rhizoma Solani tuber osi 200g, gauze filter and remove residue after boiling 20 minutes to Rhizoma Solani tuber osi 20%.)
Glucose 20g/L, ammonium sulfate 5g/L, agar 2%.
PH is natural, at sterilizing 121 DEG C 20 minutes.
Slant culture temperature 25 DEG C.
Inclined-plane seed can be placed in preservation 2 months at 0 DEG C of refrigerator, is forwarded to Shake flask medium during use.
(2) shaking flask
The same inclined-plane of Shake flask medium, but it is not added with agar.Shaking flask uses 500ml shaking flask, fills liquid 50ml.
Shaking flask temperature 30 DEG C, shaking flask rotating speed 100rpm
(3) first order seed
First order seed uses 20L fermentation tank, liquid amount 70%
Seed culture medium: Testa Tritici 15g/L, Semen Maydis powder 15g/L, ammonium sulfate 5g/L, KH2PO42g/L, regulates pH6.5.
By seed culture medium at 115 DEG C of sterilizing 15min, inoculating trichoderma kind after being cooled to 30 DEG C, inoculum concentration is 10%, cultivates 36h, prepares primary seed solution for 30 DEG C.
(4) secondary seed
Secondary seed uses 500L, liquid amount 70%
Culture medium and the same first order seed of condition of culture.
Incubation time 24h.
2, fermentation culture
Dreg (Semen Maydis grit) after the citric acid fermentation using Semen Maydis to be raw material.Fermentation culture is carried out on 2500L multifunctional reaction tank.
Semen Maydis grit 300kg, is crushed to 20-40 mesh；Semen Maydis powder 10kg, ammonium sulphate 5kg, Semen sojae atricolor powder 2kg, KH2PO42kg
Retort is initially charged bottom water 1000L, above-mentioned supplementary material is separately added in retort and is slowly stirred, mend and be filled with water to about 1500L.
115 DEG C of sterilizing 20min after mix homogeneously.
It is cooled to 25 DEG C and starts inoculation, access secondary seed 10%, stir culture 48h at 25 DEG C, be passed through pure air 1.0M3/min。
3, enzymolysis and extraction
After cultivation terminates, mend and add water to 2000L, regulate pH7.2, be warming up to 50 DEG C and be stirred enzymolysis, maintain temperature 4h.
After enzymolysis terminates, filter and remove liquid, and add the washing of a small amount of water.Waste liquid and washings merge, and carry out recycling treatment.
4, bacterial manure makes due to the enzymatic hydrolysis of preceding processes, water liquid is available for utilizing containing a large amount of carbon sources, nitrogen source, band slag hydrolyzed solution is through extruding, solid content dried for standby, liquid enters fermentation tank after composition allotment and pH are allocated, and inoculation colloid or hay, the bacillus cereus such as short and small ferment, and filter and obtain thalline after terminating, add in the most dried solid material, dry after spice or cold drying obtains ecological bacterial manure.
Embodiment 2
1, Trichoderma spp. seed culture
Cultivate according to inclined-plane, shaking flask, first order seed, secondary seed.Culture process is as follows:
(1) inclined-plane:
Culture medium: (every 1000ml water adds fresh Rhizoma Solani tuber osi 200g, gauze filter and remove residue after boiling 30 minutes to Rhizoma Solani tuber osi 20%.)
Glucose 20g/L, ammonium sulfate 5g/L, agar 2%.
PH is natural, at sterilizing 121 DEG C 20 minutes.
Slant culture temperature 30 DEG C.
Inclined-plane seed can be placed in preservation 2 months at 4 DEG C of refrigerator, is forwarded to Shake flask medium during use.
(2) shaking flask
The same inclined-plane of Shake flask medium, but it is not added with agar.Shaking flask uses 500ml shaking flask, fills liquid 100ml.
Shaking flask temperature 30 DEG C, shaking flask rotating speed 150rpm
(3) first order seed
First order seed uses 20L fermentation tank, liquid amount 70%
Seed culture medium: Testa Tritici 15g/L, Semen Maydis powder 15g/L, ammonium sulfate 5g/L, KH2PO42g/L, regulates pH7.0
By seed culture medium at 125 DEG C of sterilizing 30min, inoculating trichoderma kind after being cooled to 30 DEG C, inoculum concentration is 10%, cultivates 48h, prepares primary seed solution for 30 DEG C.
(4) secondary seed
Secondary seed uses 500L, liquid amount 70%
Culture medium and the same first order seed of condition of culture.
Incubation time 36h.
2, fermentation culture
Dreg (Semen Maydis grit) after the citric acid fermentation using Semen Maydis to be raw material.Fermentation culture is carried out on 2500L multifunctional reaction tank.
Semen Maydis grit 300kg, is crushed to 20-40 mesh；Semen Maydis powder 10kg, ammonium sulphate 5kg, Semen sojae atricolor powder 2kg, KH2PO4 2kg
Retort is initially charged bottom water 1000L, above-mentioned supplementary material is separately added in retort and is slowly stirred, mend and be filled with water to about 1500L.
115 DEG C of sterilizing 20min after mix homogeneously.
It is cooled to 25 DEG C and starts inoculation, access secondary seed 10%, stir culture 60h at 30 DEG C, be passed through pure air 1.0M3/min。
3, enzymolysis and extraction
After cultivation terminates, mend and add water to 2000L, regulate pH7.2, be warming up to 50 DEG C and be stirred enzymolysis, maintain temperature 4h.
After enzymolysis terminates, filter and remove liquid, and add the washing of a small amount of water.Waste liquid and washings merge, and carry out recycling treatment.
4, bacterial manure makes due to the enzymatic hydrolysis of preceding processes, water liquid is available for utilizing containing a large amount of carbon sources, nitrogen source, band slag hydrolyzed solution is through extruding, solid content dried for standby, liquid enters fermentation tank after composition allotment and pH are allocated, and inoculation colloid or hay, the bacillus cereus such as short and small ferment, and filter and obtain thalline after terminating, add in the most dried solid material, dry after spice or cold drying obtains ecological bacterial manure.
Claims (10)
1. one kind rich in polysaccharide, rich in the production method of ecological bacterial manure of dissolving phosphor and dissolving potassium, it is characterised in that comprise the steps:
1) Trichoderma spp. seed culture: include that inclined-plane, shaking flask, first order seed, secondary seed are cultivated；
2) fermentation culture: be initially charged bottom water in retort, is separately added into raw material, adjuvant in retort and is slowly stirred, and supplements water, sterilizing after mix homogeneously；Cooling, starts inoculation, accesses secondary seed, stir culture 48h-60h at 25 DEG C-30 DEG C, is passed through pure air；Described raw material is the dreg after the citric acid fermentation using Semen Maydis to be raw material, is crushed to 20-40 mesh；
3) enzymolysis and extraction: cultivate after terminating, adds water, regulates pH, heats up, and is stirred enzymolysis, maintains temperature；After enzymolysis terminates, filter and remove liquid, and add the washing of a small amount of water, waste liquid and washings merging, carry out recycling treatment.
2. the production method of ecological bacterial manure as claimed in claim 1, it is characterised in that comprise the steps:
1) Trichoderma spp. seed culture
Cultivate including inclined-plane, shaking flask, first order seed, secondary seed；
2) fermentation culture
Retort is initially charged bottom water 1000L, raw material, adjuvant are separately added in retort and are slowly stirred, mend and be filled with water to 1500L, 115 DEG C of sterilizing 20min after mix homogeneously；It is cooled to 25 DEG C and starts inoculation, access secondary seed 10%, stir culture 48h-60h at 25 DEG C-30 DEG C, be passed through pure air 1.0M3/min；Described raw material is the dreg after the citric acid fermentation using Semen Maydis to be raw material, and 300kg is crushed to 20-40 mesh；
3) enzymolysis and extraction
After cultivation terminates, mend and add water to 2000L, regulate pH7.2, be warming up to 50 DEG C and be stirred enzymolysis, maintain temperature 4h；After enzymolysis terminates, filter and remove liquid, and add the washing of a small amount of water, waste liquid and washings merging, carry out recycling treatment.
3. the production method of ecological bacterial manure as claimed in claim 1 or 2, it is characterised in that described inclined-plane includes:
Culture medium: Rhizoma Solani tuber osi 20%；Glucose 20g/L, ammonium sulfate 5g/L, agar 2%；PH is natural, at sterilizing 121 DEG C 20 minutes；Slant culture temperature 25～30 DEG C；Inclined-plane seed can be placed in preservation 2 months at 0-4 DEG C of refrigerator, is forwarded to Shake flask medium during use.
4. the production method of ecological bacterial manure as claimed in claim 3, it is characterised in that described Rhizoma Solani tuber osi obtains by the following method: every 1000ml water adds fresh Rhizoma Solani tuber osi 200g, gauze filter and remove residue after boiling 20-30 minute.
5. the production method of ecological bacterial manure as claimed in claim 1 or 2, it is characterised in that described shaking flask includes:
The same inclined-plane of Shake flask medium, but it is not added with agar.Shaking flask uses 500ml shaking flask, fills liquid 50-100ml；Shaking flask temperature 30 DEG C, shaking flask rotating speed 100～150rpm.
6. the production method of ecological bacterial manure as claimed in claim 1 or 2, it is characterised in that described first order seed includes:
First order seed uses 20L fermentation tank, liquid amount 70%；Seed culture medium: Testa Tritici 15g/L, Semen Maydis powder 15g/L, ammonium sulfate 5g/L, KH2PO42g/L, regulates pH6.5-7.0；By seed culture medium at 115-125 DEG C of sterilizing 15-30min, inoculating trichoderma kind after being cooled to 30 DEG C, inoculum concentration is 10%, cultivates 36-48h, prepares primary seed solution for 30 DEG C.
7. the production method of ecological bacterial manure as claimed in claim 1 or 2, it is characterised in that described secondary seed includes that secondary seed uses 500L, liquid amount 70%；Culture medium and the same first order seed of condition of culture；Incubation time 24-36h.
8. the production method of bacterial manure as claimed in claim 1 or 2 ecological, it is characterised in that step 2) described in adjuvant be Semen Maydis powder 10kg, ammonium sulphate 5kg, Semen sojae atricolor powder 2kg, KH2PO42kg。
9. the production method of bacterial manure as claimed in claim 1 or 2 ecological, it is characterised in that step 2) in fermentation culture carry out on 2500L multifunctional reaction tank.
10. the production method of ecological bacterial manure as claimed in claim 1 or 2, it is characterized in that bacterial manure makes due to the enzymatic hydrolysis of preceding processes, water liquid is available for utilizing containing a large amount of carbon sources, nitrogen source, band slag hydrolyzed solution is through extruding, solid content dried for standby, liquid enters fermentation tank after composition allotment and pH are allocated, inoculation colloid or hay, the bacillus cereus such as short and small ferment, filter after end and obtain thalline, add in the most dried solid material, dry after spice or cold drying obtains ecological bacterial manure.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610144697.9A CN105819912A (en) | 2016-03-14 | 2016-03-14 | Production method of ecological bacterial fertilizer rich in polysaccharide, phosphate solubilizing bacteria, and potassium solubilizing bacteria |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610144697.9A CN105819912A (en) | 2016-03-14 | 2016-03-14 | Production method of ecological bacterial fertilizer rich in polysaccharide, phosphate solubilizing bacteria, and potassium solubilizing bacteria |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN105819912A true CN105819912A (en) | 2016-08-03 |
Family
ID=56987261
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610144697.9A Pending CN105819912A (en) | 2016-03-14 | 2016-03-14 | Production method of ecological bacterial fertilizer rich in polysaccharide, phosphate solubilizing bacteria, and potassium solubilizing bacteria |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105819912A (en) |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101759494A (en) * | 2009-10-21 | 2010-06-30 | 山东光大肥业科技有限公司 | Diseases-prevention organic fertilizer prepared by edible fungi residue |
| CN103833432A (en) * | 2014-03-06 | 2014-06-04 | 党伟 | Method for producing biological bacterial fertilizer by using fungus dregs |
| CN103833467A (en) * | 2014-03-04 | 2014-06-04 | 重庆圣沛农业科技有限公司 | Organic compound fertilizer with citrus pomace and preparation method of organic compound fertilizer |
| CN105218276A (en) * | 2015-10-22 | 2016-01-06 | 山东省农业科学院农业资源与环境研究所 | The production method of the special xanthohumic acid microbial organic fertilizer of a kind of apple |
-
2016
- 2016-03-14 CN CN201610144697.9A patent/CN105819912A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101759494A (en) * | 2009-10-21 | 2010-06-30 | 山东光大肥业科技有限公司 | Diseases-prevention organic fertilizer prepared by edible fungi residue |
| CN103833467A (en) * | 2014-03-04 | 2014-06-04 | 重庆圣沛农业科技有限公司 | Organic compound fertilizer with citrus pomace and preparation method of organic compound fertilizer |
| CN103833432A (en) * | 2014-03-06 | 2014-06-04 | 党伟 | Method for producing biological bacterial fertilizer by using fungus dregs |
| CN105218276A (en) * | 2015-10-22 | 2016-01-06 | 山东省农业科学院农业资源与环境研究所 | The production method of the special xanthohumic acid microbial organic fertilizer of a kind of apple |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN101983952B (en) | Method for producing organic fertilizer by using municipal sewage sludge | |
| CN103626575B (en) | A kind of natural pond liquid produces the method for organic-inorganic complex fertilizer | |
| CN107935763A (en) | For salinisation alkaline land improving microbial-bacterial fertilizer and preparation method thereof | |
| CN102260104B (en) | Method for producing biological organic fertilizer by fermenting sludge | |
| CN105272761A (en) | Selenium-enriched biological organic fertilizer and production method thereof | |
| CN105482827A (en) | Soil conditioner with function of heavy metal-polluted soil restoration | |
| CN106397003A (en) | Agricultural organic fertilizer, and preparation method thereof | |
| CN105384534B (en) | A kind of liquid organic biofertilizer and preparation method thereof | |
| CN103664260B (en) | Organic fertilizer fermenting agent and preparation method thereof | |
| CN102910976A (en) | Biological organic fertilizer produced from fermented traditional Chinese medicine residue and method for producing fertilizer | |
| CN104446683A (en) | Comprehensive treatment technology for municipal domestic sludge | |
| CN103772042A (en) | Mushroom dreg fermented organic fertilizer and preparation method thereof | |
| CN106083375A (en) | A kind of method utilizing biogas slurry to prepare salt-soda soil irrigation liquid fertilizer | |
| CN104355842A (en) | Strawberry coated fertilizer rich in biological organic matter and preparation method of strawberry coated fertilizer | |
| KR101264876B1 (en) | Method for producing liquid fertilizer using animal raw blood | |
| CN106316674A (en) | Biological carbon fertilizer capable of promoting crop growth | |
| CN106365713A (en) | Biochar fertilizer with effect of balancing fertilizer-water distribution | |
| CN103145457A (en) | Method for producing bio-organic fertilizer by using sheep manure | |
| CN106748336A (en) | Liquid fertilizer containing streptococcus acidi lactici fermented solution | |
| CN103385358B (en) | Chinese medicine residue-fermented feed and preparation method thereof | |
| CN112745173A (en) | Cadmium-reducing soil improvement functional organic fertilizer | |
| CN106673856A (en) | Multifunctional full-nutritional fertilizer and preparation method thereof | |
| CN106905048A (en) | Using the Food waste treatment method and kitchen waste compost conditioner of spent bleaching clay | |
| CN105819912A (en) | Production method of ecological bacterial fertilizer rich in polysaccharide, phosphate solubilizing bacteria, and potassium solubilizing bacteria | |
| CN110066204A (en) | A kind of organic biofertilizer production method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20160803 |
|
| RJ01 | Rejection of invention patent application after publication |