CN105815321A - Propiconazole-containing pesticide composition for preventing and treating wheat head blight and application of propiconazole-containing pesticide composition - Google Patents

Propiconazole-containing pesticide composition for preventing and treating wheat head blight and application of propiconazole-containing pesticide composition Download PDF

Info

Publication number
CN105815321A
CN105815321A CN201610244977.7A CN201610244977A CN105815321A CN 105815321 A CN105815321 A CN 105815321A CN 201610244977 A CN201610244977 A CN 201610244977A CN 105815321 A CN105815321 A CN 105815321A
Authority
CN
China
Prior art keywords
propiconazole
wheat
jingganmycin
agriculture
pesticide
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CN201610244977.7A
Other languages
Chinese (zh)
Other versions
CN105815321B (en
Inventor
潘建碧
周明国
段亚冰
王建新
侯毅平
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Nanjing Jixing Biotechnology Development Co Ltd
Original Assignee
Nanjing Jixing Biotechnology Development Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanjing Jixing Biotechnology Development Co Ltd filed Critical Nanjing Jixing Biotechnology Development Co Ltd
Priority to CN201610244977.7A priority Critical patent/CN105815321B/en
Publication of CN105815321A publication Critical patent/CN105815321A/en
Application granted granted Critical
Publication of CN105815321B publication Critical patent/CN105815321B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
    • A01N43/647—Triazoles; Hydrogenated triazoles
    • A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
    • A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
    • A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
    • A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
    • A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Pest Control & Pesticides (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention relates to a pesticide composition for preventing and treating wheat head blight. The pesticide composition contains 1-150 parts of validamycin and 1-20 parts of propiconazole. The pesticide composition has an obvious synergistic effect when being used for preventing and treating wheat head blight; the prevention and treatment effect on the wheat head blight can be obviously improved; the capability of human for preventing and controlling the gibberellic disease of triticeae crops is improved; the pollution level of cereal DON toxin is significantly reduced; the dosage is reduced; the cost is reduced; the environment pollution and the pesticide residue are reduced; the medicine resistance of pathogenic fungus on propiconazole is relayed; the fusarium blight is prevented and treated; the lasting period of validamycin is shortened; powdery mildew, a rust disease, leaf blight and banded sclerotial blight are treated; the labor cost of application of the pesticide is reduced; the dosage of the chemical pesticide is greatly reduced; and the pesticide composition is friendly to environment.

Description

A kind of for prevent and treat gibberella zeaze petch of wheat and barley containing propiconazole composition pesticide and application thereof
[technical field]
The invention belongs to technical field of pesticide.More particularly it relates to an for the composition pesticide preventing and treating gibberella zeaze petch of wheat and barley, further relate to the purposes of described composition pesticide.
[background technology]
Semen Tritici aestivi and Fructus Hordei Vulgaris are the most most important frumentum raise crops, are also one of Chinese staple food crops.Cereal crops usually face the threat of the fungal diseases such as head blight, powdery mildew, banded sclerotial blight, rust and leaf blight.At world's frumentum growing area, these diseases all can occur, have a strong impact on the yield and quality of frumentum agricultural product, especially can not only be caused destructive production loss by the microbial gibberella zeaze petch of wheat and barley of reaping hook, and also can produce deoxynivalenol (Trichothecenes in the grain infected, and the toxin such as 6-(10-hydroxy-6-oxo-trans-1-undecenyl)-.beta.-resorcylic acid lactone (Zearalenon) DON), therefore the food qualities such as fermentation, wine brewing, the health of harm humans and animals are had a strong impact on.
Deoxynivalenol toxin, including DON, 3ADON and 15ADON toxin, all has acute and chronic toxicity to humans and animals.Wherein DON toxin is because of its stable chemical nature, is heated and does not decomposes, and is infecting the Wheat And Barley of gibberellic hypha and processed food thereof and be widely present in the sick paddy animal product as feedstuff infecting gibberellic hypha.Known DON toxin causes that the cardinal symptom of person poultry poisoning has Nausea and vomiting, dizziness, drowsiness, headache, brothers are numb, malaise, immunity degradation, severe patient visible breathing, pulse, body temperature, the fluctuation of blood pressure, and hemorrhage, miscarry, even result in death, and mankind's Kaschin-Beck disease (Kaschin-Beckdisease, KBD) can be caused and there is obvious fetal toxicity and teratogenesis, carcinogenesis.Due to the serious harm to human health of the DON toxin, in October, 1973 the World Food Programme of the United Nations (FAO) and World Health Organization (WHO) (WHO) are in the joint conference about food additive and pollutant that Geneva is held, fusarium toxin is listed in the 5th in 16 problems of priority research, many countries preventing and treating as important research topic using head blight the most always by decision.
Meanwhile, the health organization of many international organizations and country has been formulated corresponding laws and regulations and has strictly been limited DON content of toxins in food.FAO specifies that the content of DON in grain is necessarily less than the DON content of toxins that 1mg/kg, WHO specify in grain and feedstuff and the most not can exceed that the standard of 1mg/kg and 5mg/kg.European Union's food security standard requires DON content of toxins less than 0.75mg/kg in edible wheat grain, less than 0.5mg/kg in bread and other food.National Specification content of toxins of DON in edible Semen Tritici aestivi flour and Semen Maydis that China's Ministry of Public Health is formulated must not exceed 1mg/kg.
Gibberella zeaze petch of wheat and barley be one can by multiple Fusarium spp. or the bud-rot claiming Fusariumsp (Fusariumspp.) cause, Seedling is withered and fringe is rotten/the withered ascomycetes disease of fringe.Its cause of disease includes nearly 20 kinds such as Fusarium graminearum (F.graminearum), Asia Fusarium spp. (F.asiaticum), fusarium culmorum (F.culmorum), fusarium avenaceum (F.avenaceum), fusarium moniliforme (F.moniliforme), Fusarium nivale (F.nivale).The wheat scab of China's most area is caused by Fusarium graminearum (F.graminearum) and Asia Fusarium spp. (F.asiaticum) mixed population, wherein south Mai Qu is based on Asia Fusarium spp., and north Mai Qu is based on Fusarium graminearum.
Owing to not having so far it can be found that have immunity or high anti-cereal crops kind or genetic resources to Fusarium spp., antibacterial is used to become the only effective gibberella zeaze petch of wheat and barley Control Technology.Since the nearly century; people have carried out the antibacterial R&D work being largely used to wheat and barley fungal disease; successively develop the protective fungicides such as inorganic fungicide, organosulfureous fungicide and aromatic hydrocarbons; the selectivity antibacterial such as benzimidazole germicide, ergosterol biosynthesis inhibitor, cytochrome b inhibitor and the succinate dehydrogenase inhibitors developed after particularly 20 sixties in century; the popular harm making many important diseases of wheat crops is effectively controlled, such as cereal crops smut, powdery mildew, rust, leaf blight, eye spot etc..But, deoxynivalenol (DON) toxin produced due to Fusarium spp. (Fusariumspp.) has the function of virulence factor, increase the drug resistance effect to various sterilization agent, so that most antibacterial is the most very good to the prevention effect of gibberella zeaze petch of wheat and barley.Therefore, the most effectively control cereal crops head blight, be the great social need of current Ensuring Food Safety and food safety.
The DON endotoxin contamination level of corn of falling ill depends on corn infected pathogen bacterium amount and thalline toxin biosynthesis ability.The present invention is directed to prevent and treat outside Now Domestic the situation that major antibacterial agent preventive effect is the most very good, DON endotoxin contamination often exceeds edible safety criterion of gibberella zeaze petch of wheat and barley, research also finds that Fusarium spp. exists two kinds of 'beta '-tubulin (β of benzimidazole germicide first1-and β2-) receptor, and the hereditary negative regulation mechanism of the antagonism property of medicine and drug sensitivity, find wherein to form β2240 amino acids phenylalanine of-tubulin receptor are the main causes reducing drug sensitivity, 167 amino acids point mutation can cause carbendazim to lose prevention effect, and thalline DON toxin synthesis capability, mycelia rate of propagation and pathogenecity are greatly enhanced, reduce the prevention effect of antibacterial；It addition, research also finds ergosterol biosynthesis inhibitor (EBI) receptor-cytochrome P of Fusarium spp.450Monooxygenase, it is also possible to reduce the drug sensitivity to EBI by encoding gene process LAN and point mutation or develop immunity to drugs.Therefore, although although some antibacterial show very high activity at laboratory, but then need higher dosage at Field information, as every mu needs to use 40～50 grams of (600-750ga.i./hm2) benzimidazole germicide of effective above composition or 15～20 grams of (225-300ga.i./hm2) EBI of effective above composition, the gibberella zeaze petch of wheat and barley that could obtain about 70% controls effect.
Along with long-term a large amount of selectivity antibacterial using these mechanisms of action single, the pathogenic fungi colony of nature occurs in that Drug resistance, using effect decline year by year.Inventor passes through the monitoring for resistance of nearly 30 years, finding that East China has formed dominant group to the Fusarium spp. that the benzimidazole germicides such as carbendazim develop immunity to drugs, the benzimidazole germicide such as conventional carbendazim, thiophanate substantially loses the preventing and treating to gibberella zeaze petch of wheat and barley and is worth.Simultaneously also as it is more than 5 times of sensitive strain that Drug resistance pathogenic bacteria produces the ability of DON toxin, the harm of Chinese gibberella zeaze petch of wheat and barley and the risk of food safety are day by day increased the weight of.Peasant, in order to reduce production loss, often doubles to use the antibacterial such as carbendazim, has been further exacerbated by Drug resistance, pesticide residues, environmental pollution and food-safety problem.
To this end, the present inventor is on the basis summing up prior art, by lot of experiments and analysis, finally complete the present invention.
[summary of the invention]
[solving the technical problem that]
It is an object of the invention to provide a kind of composition pesticide for preventing and treating gibberella zeaze petch of wheat and barley.
It is a further object to provide the purposes of described composition pesticide.
[technical scheme]
The present invention is achieved through the following technical solutions.
The present invention relates to a kind of composition pesticide for preventing and treating gibberella zeaze petch of wheat and barley.
Described composition pesticide is made up of according to weight ratio 1～150:1～20 with propiconazole jingganmycin.
A preferred embodiment of the invention, described composition pesticide is made up of according to weight ratio 5～65:3～18 with propiconazole jingganmycin.
According to the another kind of preferred implementation of the present invention, described composition pesticide is made up of according to weight ratio 10～20:6～16 with propiconazole jingganmycin.
The invention still further relates to a kind of disinfectant use in agriculture for preventing and treating gibberella zeaze petch of wheat and barley.Described disinfectant use in agriculture contains composition pesticide described in 0.1～90.0% by weight, and surplus is acceptable carrier and/or auxiliary agent in pesticide.
A preferred embodiment of the invention, described disinfectant use in agriculture contains composition pesticide described in 10～75.0% by weight, and surplus is acceptable carrier and/or auxiliary agent in pesticide.
According to the another kind of preferred implementation of the present invention, described disinfectant use in agriculture contains composition pesticide described in 26～58.0% by weight, and surplus is acceptable carrier and/or auxiliary agent in pesticide.
According to the another kind of preferred implementation of the present invention, described disinfectant use in agriculture is suspending agent, aqueous emulsion, microemulsion, wettable powder or water dispersion granule.
According to the another kind of preferred implementation of the present invention, described carrier is that one or more are selected from water, attapulgite, Kaolin or precipitated calcium carbonate.
According to the another kind of preferred implementation of the present invention, described auxiliary agent is selected from ethanol, methanol, ethylene glycol, propylene glycol, NNO-1, NNO-7, xanthan gum, Polyethylene Glycol, glycerol, pulls open powder, sodium lauryl sulphate, dodecylbenzene sodium sulfonate, ammonium sulfate, alkylphenol polyoxyethylene, alkylphenol-polyethenoxy base ether phosphate, polyoxyethylene carboxylate, benzoic acid, sodium lignin sulfonate, carboxymethyl cellulose or polyvinyl alcohol.
Another kind of preferred implementation according to the present invention, described gibberella zeaze petch of wheat and barley is wheat scab and barley scab, the Fusarium pathogenic fungi (Fusariumspp.) including carbendazim resistance Fusarium spp. the wheat crops bud-rot that causes, Seedling is withered, fringe is rotten or withered fringe.
The present invention is described in more detail below.
The present invention relates to a kind of composition pesticide for preventing and treating gibberella zeaze petch of wheat and barley.
Described composition pesticide is made up of according to weight ratio 1～150:1～20 with propiconazole jingganmycin.
The propiconazole used in the present invention, English common name propiconazole, its chemical structural formula is as follows:
Propiconazole is belonging to the triazole bactericidal agent in sterol inhibitor, has treatment and protection dual function.Its mechanism of action is to affect the biosynthesis of sterol, makes the cell membrane function of pathogen be destroyed, and ultimately results in cell death, thus plays sterilization, diseases prevention and the effect cured the disease.This medicament has the features such as fungicidal spectrum is wide, activity is high, sterilization speed is fast, lasting period length, Uptake and translocation are strong, can be absorbed by root, stem, leaf portion, and can soon in plant on conduct, the fungal disease that preventing and treating ascomycetes, basidiomycetes and Fungi Imperfecti cause, particularly take-all, powdery mildew, rust, root rot, bakanae disease of rice, banded sclerotial blight, sigatoka are had specially good effect, but invalid to oomycetes disease.This medicament has become as large-tonnage triazole type emerging broad-spectrum germicide Representative Cultivars in the world at present.
The jingganmycin (valida) that the present invention uses, English common name Validamycin or jinggangmycin, its chemical structural formula is as follows:
Jingganmycin is a kind of actinomycetic secondary metabolites, containing A, B, C, D, E, F six kinds similar glycosaminoglycan glycoside derivant.Numerous studies show both at home and abroad, and component A is the main active component of jingganmycin.Therefore, the jingganmycin related in the present invention is jinggangmycin A.
Jingganmycin Rhizoctonia fungus (Rhizoctoniaspp.) top hyphal development in vitro performance specificity interference basidiomycetes.Therefore, jingganmycin is since 20 century 70s are found, prevent and treat the plant disease that rhizoctonia causes always as agricultural antibiotic in specific manner, or become with the pesticide processing of preventing and treating other plant harmful organism that built agent is double controls rhizoctonia (Rhizoctoniaspp.) disease.These rhizoctonia diseases mainly include rice sheath blight disease, wheat sharp eyespot and other crop banded sclerotial blight or damping-off.In recent years, jingganmycin is found again to can be also used for preventing and treating the false smut that a kind of Fungi Imperfecti (Ustilaginoideavirens) being difficult to cultivate causes.
Owing to valida or jingganmycin show in vitro lower, rhizoctonia is had specialization antibacterial activity; the Fusarium spp. causing head blight do not had antibacterial activity; therefore; people are always with rhizoctonia as object in decades; the selectivity mechanism of research jingganmycin, is found to have Validamycin/jingganmycin chaff pyrenomycetes inositol and Trehalose Metabolism, thus destroys the structure of cell wall; stop rhizoctonia to infect plant, there is good protective effect.Recent decades, this antibiotic was limited to for preventing and treating crops rhizoctonia disease, especially rice sheath blight disease always.
It is known that act on single specialization antibacterial easily filter out Drug resistance pathogen, after extensively application, using value will be lost soon.The present inventor studies the rhizoctonia resistance risk to selectivity jingganmycin from the eighties in 20th century, find that jingganmycin is different from other any selectivity antibiotic and chemical bactericide that resistance risk is high, under laboratory and field condition, all cannot screen jingganmycin resistance rhizoctonia.Thus inferring, jingganmycin, in addition to the known inositol of interference and trehalose biosynthesis, is likely present other special resistant effect mechanism.
The present inventor, on the basis of research Fusarium spp. DON toxin biosynthesis pathway and regulatory mechanism, has carried out the biosynthetic a large amount of screening compounds of suppression DON toxin.In screening process, in fact it has surprisingly been found that jingganmycin is under certain treatment dosage, it is possible to the biochemical reaction in strong inhibition fusarium graminearum DON toxin biosynthesis approach in early days.Reduce, therefore it provides a kind of, jingganmycin and the propiconazole composition for preventing and controlling gibberella zeaze petch of wheat and barley technology that DON pollutes, become the free-revving engine of the present invention.
The present inventor has carried out jingganmycin/valida suppression Fusarium spp. virulence factor DON synthesis first, and screen at the synergistic formula of field control gibberella zeaze petch of wheat and barley with the mixture of other various antibacterial, creatively find that jingganmycin and propiconazole composition for preventing and controlling wheat crops head blight have notable potentiation, reduce DON endotoxin contamination and the beneficial effect of propiconazole consumption is greatly reduced.
In the composition pesticide of the present invention, if the amount of jingganmycin is in the range of 1～150 weight portions, and when the amount of propiconazole is less than 1 weight portion, then can reduce the prevention effect to head blight；When the amount of propiconazole is more than 20 weight portion, then can be inhibited to wheat growth.Therefore, the amount of propiconazole be 1～20 weight portions be rational.
Similarly, if the amount of propiconazole is in the range of 1～20 weight portions, and when the amount of jingganmycin is less than 1 weight portion, then can lose and work in coordination with the potentiation preventing and treating head blight, reducing endotoxin contamination level with propiconazole；When the amount of jingganmycin is more than 150 weight portion, then can increase cost, reduce to work in coordination with propiconazole and prevent and treat head blight and reduce the potentiation of endotoxin contamination；Therefore, the amount of jingganmycin be 1～150 weight portions be appropriate.
Preferably, described composition pesticide is made up of according to weight ratio 5～65:3～18 with propiconazole jingganmycin.
It is highly preferred that described composition pesticide is made up of according to weight ratio 10～20:6～16 with propiconazole jingganmycin.
Jingganmycin and the propiconazole that the present invention uses is all product sold in the market, such as by Qianjiang Biochemistry Co., Ltd., Zhejiang Prov. 3%, 5%A, 20%, 40%A, 60%A validamycin powder agent and water preparation；The propiconazole produced by Maxime chemical company of Israel.
The invention still further relates to a kind of agricultural bactericidal agent formulation for preventing and treating gibberella zeaze petch of wheat and barley.Described disinfectant use in agriculture contains composition pesticide described in 0.1～90.0% by weight, and surplus is acceptable carrier and/or auxiliary agent in pesticide.
In the present invention, farm chemical carrier or auxiliary agent are it should be understood that be used for improving medicament physicochemical property in Pesticide formulation or use, improve drug effect, it is simple to the auxiliary substance of the performances such as transport storage, such as wetting agent, emulsifying agent, dispersant, sticker, stabilizer or synergist.According to the present invention, every have these performances and the disinfectant use in agriculture to the present invention does not has the chemical substance of any negative effect to may be used to the present invention, and they are all within protection scope of the present invention.
E.g. one or more are selected from water, attapulgite, Kaolin or precipitated calcium carbonate to the carrier that the present invention uses.In addition, the present invention can also use other carrier normally used in the art meeting application claims.
The auxiliary agent that the present invention uses is selected from ethanol, methanol, ethylene glycol, propylene glycol, NNO-1 (chemical name: naphthalene sulfonic acid-formaldehyde condensation product sodium salt), NNO-7 (chemical name: naphthalene sulfonic acid-formaldehyde condensation product calcium salt), xanthan gum, Polyethylene Glycol, glycerol, pull open powder, sodium lauryl sulphate, dodecylbenzene sodium sulfonate, ammonium sulfate, alkylphenol polyoxyethylene, alkylphenol-polyethenoxy base ether phosphate, polyoxyethylene carboxylate, benzoic acid, sodium lignin sulfonate, carboxymethyl cellulose or polyvinyl alcohol.The auxiliary agent that the present invention uses is extensive product sold in the market, such as by Fei Huang Chemical Co., Ltd. of Xinyi City with trade name NNO-1, the diffusant that NNO-7 sells, pull open, with trade name, the sodium butylnaphthalenesulfonate emulsifying that powder is sold by Hubei emerging milky way Chemical Co., Ltd., diffusion and penetrating agent, the alkylphenol polyoxyethylene sold by Shanghai loyalty Fine Chemical Co., Ltd, the alkylphenol-polyethenoxy base ether phosphate sold by Guangzhou Xi Lu Chemical Co., Ltd., the polyoxyethylene carboxylate sold by Liangshan, Shandong Province Hong Tai epoxy soybean oil company limited, the carboxymethyl cellulose sold by Ma Shan auxiliary reagent factory, Pingxiang, Jiangxi Province.In addition, the present invention can also use other auxiliary agent normally used in the art meeting application claims.
In the present invention, in described disinfectant use in agriculture, when the amount of described composition pesticide is less than 0.1%, then can reduce protection effect or lose potentiation；When the amount of described composition pesticide is more than 90.0%, then can lose potentiation or reduce protection effect；Therefore, the amount of described composition pesticide is 0.1～90.0% to be rational.
Preferably, described disinfectant use in agriculture contains composition pesticide described in 10～75.0% by weight, and surplus is acceptable carrier and/or auxiliary agent in pesticide.
It is highly preferred that described disinfectant use in agriculture contains composition pesticide described in 26～58.0% by weight, surplus is acceptable carrier and/or auxiliary agent in pesticide.
In actual applications, according to method well known to those skilled in the art, the bactericidal composition of the present invention can be configured at the dosage form agriculturally generally used, such as suspending agent, aqueous emulsion, microemulsion, wettable powder or water dispersible granules with carrier and/or auxiliary agent.
When preparing suspending agent, it is possible to use carrier or auxiliary agent e.g. selected from alkylnaphthalene sulfonate, polycarboxylate or the dispersant of lignosulfonates；Selected from alkylphenol-polyethenoxy base ether phosphate, phenethyl phenol polyethenoxy base ether phosphate, alkyl sulfate, alkylsulfonate or the wetting agent of naphthalene sulfonate；Selected from xanthan gum, aluminium-magnesium silicate, bentonitic thickening agent；Selected from benzoic acid or the preservative of sodium benzoate；Silicone defoamer；Antifreezing agent selected from glycerol, carbamide, ethylene glycol or propylene glycol.
When preparing aqueous emulsion, it is possible to use carrier or auxiliary agent e.g. selected from Nongru-700, agriculture breast 2201, Span-60 or the emulsifying agent of emulsifying agent T-60；Solvent selected from dimethylbenzene, toluene or Ketohexamethylene；Selected from triphenyl phosphite or the stabilizer of epoxychloropropane；Antifreezing agent selected from ethylene glycol, propylene glycol, glycerol or carbamide；Thickening agent selected from aluminium-magnesium silicate, bentonite or xanthan gum；Selected from benzoic acid or the preservative of sodium benzoate.
When preparing microemulsion, it is possible to use carrier or auxiliary agent e.g. selected from calcium dodecyl benzene sulfonate (agriculture breast 500), Nongru-700, agriculture breast 2201, this dish-60, tween 80 or the emulsifying agent of TX-10；Cosolvent selected from methanol, isopropanol, n-butyl alcohol or ethanol；Solvent selected from dimethylbenzene, toluene, Ketohexamethylene or N-Methyl pyrrolidone；Selected from triphenyl phosphite or the stabilizer of epoxychloropropane.
When preparing wettable powder, it is possible to use carrier or auxiliary agent e.g. selected from polycarboxylate, lignosulfonates or the dispersant of alkylnaphthalene sulfonate；Wetting agent selected from alkylsulfonate, alkyl sulfate or naphthalene sulfonate；Filler selected from precipitated calcium carbonate, Pulvis Talci, kieselguhr, Kaolin or attapulgite.
When preparing water dispersible granules, it is possible to use carrier or auxiliary agent e.g. selected from polycarboxylate, lignosulfonates or the dispersant of alkylnaphthalene sulfonate；Wetting agent selected from alkyl sulfate, polyoxyethylene alcohol, alkylsulfonate or naphthalene sulfonate；Disintegrating agent selected from citric acid, ammonium sulfate, glucose, carbamide or sodium bicarbonate；Selected from corn starch, microcrystalline Cellulose class or Diatomite binder；Selected from precipitated calcium carbonate, meerschaum, kieselguhr, Kaolin, attapulgite or talcous filler.
Below by its result of study that is described in detail respectively.
I, the reactive compound screening of suppression Fusarium spp. growth
The present invention uses the conventional method of fungicide bioassay, and with aquesterilisa and methanol, jingganmycin and propiconazole are configured to 2mg/mL mother solution respectively, and medicament carbendazim active compound is dissolved in 0.1M/L hydrochloric acid solution, 2-cyano-3-amino-3-phenylancryic acetate is dissolved in methanol in comparison, makes 2mg/mL mother solution.When potato dextrose agar (PDA) is cooled to temperature about 45 DEG C, it is separately added into jingganmycin and propiconazole to design concentration (being shown in Table 1), it is subsequently poured into culture dish and makes the flat board that different agents processes, often process 3 wares to repeat, inoculation causes the common Fusarium graminearum Fusariumgraminearum of gibberella zeaze petch of wheat and barley and the Asia wild sensitive strain of Fusarium spp. Fusariumasiaticum (being called for short sensitive strain) and carbendazim and the mycelia block of 2-cyano-3-amino-3-phenylancryic acetate resistant strain (abbreviation drug-fast strain), cultivate 4 days at temperature 25 DEG C, decussation method measures colony diameter, calculate the middle dosage (EC of different disposal suppression pathogen growth 50%50Value), and compare antibacterial activity.
Result of the test shows, jingganmycin under in vitro either the growth of single dose still both Fusarium spp. sensitive strain and drug-fast strain to cause head blight mixed with propiconazole substantially there is no inhibitory activity, only jingganmycin is up to 50 (during g/mL concentration, just has the inhibitory action of 6.5%～7.8% to two kinds of Fusarium spp. growths.But propiconazole has a similar strong inhibition effect to the sensitive strain of two kinds of Fusarium spp. and the mycelial growth of drug-fast strain, 0.25 (g/mL propiconazole processes the inhibitory action to mycelial growth can be more than 50%.Jingganmycin does not has potentiation (seeing table 1) in vitro to propiconazole suppression mycelial growth.
According to the wild sensitive strain of Asia Fusarium spp. and Fusarium graminearum and carbendazim and 2-cyano-3-amino-3-phenylancryic acetate resistant strain growth inhibition ratio under the process of propiconazole various dose, calculate the propiconazole middle dosage (EC to different drug sensitivity strain growths suppression50), found that sensitive strain, carbendazim resistance bacterial strain, the 2-cyano-3-amino-3-phenylancryic acetate resistant strain sensitivity similar to propiconazole, EC50It is that 0.18～0.28 (g/mL, result of the test is shown in Table 2.
With medicament EC50For parameter, the relatively activity of different antibacterial suppression Fusarium spp. growth, find that the activity of propiconazole is approximately carbendazim to sensitive strain activity (to two kinds of Fusarium spp. EC50It is 2 times of 0.45 (g/mL), with 2-cyano-3-amino-3-phenylancryic acetate to sensitive strain activity (to two kinds of Fusarium spp. EC50It is 0.165 (g/mL) suitable.This result explanation propiconazole has the wild sensitive strain of strong inhibition and carbendazim and the activity of 2-cyano-3-amino-3-phenylancryic acetate resistance Fusarium spp. growth, is beneficial to reduce and infects the DON endotoxin contamination level of corn and prevent and treat Drug resistance disease.
Table 1: the impact that two kinds of Fusarium spp. sensitive strains are grown by jingganmycin and propiconazole in vitro
* F.g and F.a is the abbreviation of Fusariumgraminearum and Fusariumasiaticum respectively, lower same.
Table 2: propiconazole is sensitive and carbendazim and the growth inhibitory activity of 2-cyano-3-amino-3-phenylancryic acetate resistant strain to two kinds of Fusarium spp.
II. the jingganmycin inhibitory activity to fusarium toxin biosynthesis ability
Owing to Fusarium graminearum is identical to the drug sensitivity of jingganmycin and propiconazole with Asia Fusarium spp., inventor selects toxin synthesis capability, and (unit bacterium amount synthesis DON weight, (gDON/g dry weight mycelia) stronger Asia Fusarium spp. Fusariumasiaticum is as the material of research toxin synthesis further.The carbendazim resistance Asia Fusarium spp. causing gibberella zeaze petch of wheat and barley is inoculated in 3% sweet mung bean soup of sterilizing, shakes training 10 days, centrifugal collection conidium for 25 DEG C and 12/24 hour under scattered light in temperature.By conidium by being finally 106/ mL is inoculated in potato sucrose (PS) culture fluid containing jingganmycin various dose, training is shaken under scattered light for 25 DEG C and 12/24 hour in temperature, culture is filtered after 7 and 14 days, content of toxins in detection culture fluid and measurement dry mycelial weight respectively, analyzes toxin synthesis capability (the toxin amount that unit weight mycelia produces).
Toxin determination method: culturing filtrate extracts 2 times with ethyl acetate equal-volume respectively, combining extraction liquid, vacuum distillation drying is dried to new centrifuge tube, redistillation with 1mL acetonitrile solution transfer, preserves to be measured at temperature-20 DEG C.100 (LTMS derivatization reagents (TMSI:TMCS=100:1) are added when detection, mixing 10min, add 1mL ultra-pure water, concussion layering, Aspirate supernatant be added to GC sample injection bottle again, carries out content of toxins detection by the gas chromatogram (GC-ECD) equipped with electronics capture monitor.With the DON reagent of Sigma as standard specimen, Criterion curve, calculate the DON content in culture fluid, including DON, 3ADON and 15ADON.Mycelia will be leached dry to constant weight at temperature 80 DEG C simultaneously, weigh dry mycelial weight.Additionally, shake training 7 days time, take mycelia detection toxin synthesis key gene Tri5 expression.
From table 3 experimental result it appeared that the mycelium morphology factor of gibberellic hypha extends along with incubation time and increases, but shaking training in the culture medium processed containing various dose jingganmycin, mycelium morphology factor does not has significant change with blank.Illustrate that jingganmycin does not has inhibitory action to the growth of the gibberellic hypha of liquid culture, consistent with the linear growth rate measurement result on PDA plate.But, find that the amount (μ gDON/g dry weight mycelia) of unit mycelia weight synthesis DON toxin then increases with jingganmycin treatment dosage and substantially reduces first.And the inhibitory action that DON is synthesized by jingganmycin extends with incubation time and declines, especially low concentration process fall is bigger.Illustrate jingganmycin along with the prolongation of test period it may happen that degraded, thus reduce the biosynthetic inhibitory action of contratoxin.
According to toxin synthetic gene expression horizontal analysis when processing 7 days, although finding that jingganmycin does not has harmful effect in vitro to growth and the hypha form of Fusarium spp., but can express by strong inhibition DON toxin synthesis key gene tri5 under very reduction process dosage, reduce thalline toxin biosynthesis ability, reducing DON biosynthesis, its result of the test is shown in Table 4.
Table 3: jingganmycin suppression Fusarium spp. DON toxin synthesis capability result of the test
Table 4: jingganmycin is to Fusarium spp. DON synthetic gene Tri5 Gene Expression result of the test
III. the propiconazole potentiation to jingganmycin suppression Fusarium spp. DON toxin biosynthesis ability
Propiconazole processes the cell leakage that can destroy gibberellic hypha, suppresses mycelial growth.When propiconazole processes with validamycin composition, by increasing capacitance it is possible to increase jingganmycin is absorbed by pathogenic bacteria.While research contents II measures jingganmycin suppression Fusarium spp. DON toxin biosynthesis, also measured were 0.1 (in the presence of g/mL propiconazole, the jingganmycin inhibitory action to the DON toxin biosynthesis ability of Fusariumasiaticum when cultivating 7 days and 14 days, analyzes propiconazole and jingganmycin is suppressed the biosynthetic potentiation of DON toxin.DON detection method is identical with research contents II.
Knowing from research contents I and II, 0.1 μ g/ml propiconazole individual processing has the suppression ratio close to 50% to the mycelial growth of gibberellic hypha.As can be seen from Table 5,0.1 μ g/ml propiconazole processes does not has significant inhibitory action when 7 days and 14 days to the biosynthesis ability of fusarium toxin and the toxin synthesis capability of blank, illustrate that propiconazole only has mycelial growth inhibitory activity, there is no the inhibitory action of toxin synthesis capability.But, in the presence of 0.1 μ g/ml propiconazole, the inhibitory action of DON toxin biosynthesis ability is increased substantially by the jingganmycin of each concentration for the treatment of.And along with incubation time extends, the inhibitory action decrease speed of jingganmycin contratoxin synthesis capability does not substantially less than have the control treatment of propiconazole, the potentiation especially processed low concentration jingganmycin and prolongation to become apparent from action time.Listing result according to table 3 to calculate, when 0.10 μ g/mL propiconazole, when jingganmycin processes 7 days and 14 days, to the suppression biosynthetic potentiation of fusarium toxin, the results are shown in Table 5.Explanation is understood: (1) jingganmycin has the strong effect reducing Fusarium spp. DON toxin biosynthesis ability, and propiconazole does not then have this effect by these results；(2) propiconazole has obvious potentiation to jingganmycin suppression fusarium toxin biosynthesis, and reduces with jingganmycin treatment dosage, and potentiation strengthens；(3) propiconazole can extend the inhibitory action time that fusarium toxin is synthesized by jingganmycin, and potentiation strengthens with processing time lengthening.
Table 5:0.1g/mL propiconazole is to jingganmycin suppression fusarium graminearum DON toxin synthesis synergy test result
* coefficient of synergism computational methods: when 0.1 μ g/ml propiconazole, jingganmycin contratoxin synthesis suppression ratio synthesizes suppression ratio divided by jingganmycin individual processing corresponding dosage toxin, is multiplied by 100.
Disinfectant use in agriculture suspending agent the most of the present invention processes wheat seed preventing and treating Wheat Seedling head blight and the effect of other disease and potentiation
According to jingganmycin and weight ratio 1:1.5 of propiconazole, 10:1.5,100:1.5,1:1,10:1,100:1,2:1,20:1,200:1,5:1,50:1 and 500:1, prepare respectively content be 3% (1:1.5,1:1,2:1), 5% (10:1.5,10:1,5:1), 10% (50:1,100:1,20:1), 20% (100:1.5,200:1,500:1) composition suspending agent sample.Sample preparation methods is propiconazole and the former medicine of jingganmycin mixes according to above-mentioned weight ratio, add in the solution containing 40% water, 8% ethylene glycol and mixed with propylene glycol solvent (volume ratio 1:1), 1.0%NNO-1 and 1.5%NNO-7 dispersant (production of Fei Huang Chemical Co., Ltd. of Xinyi City), 0.5% xanthan gum sticker and 1% Polyethylene Glycol by weight, finally it is supplemented to 100% with water, is crushed to pharmacy particle diameter 5 μm of 90% through sand mill.
The single dose consumption in described disinfectant use in agriculture suspending agent is used by every 100kg seed treatment, the jingganmycin of design corresponding dosage and propiconazole single dose consumption (being shown in Table 6), after sample being diluted water with medicinal liquid 5 liters by 100 kilograms of seeds the most respectively, Dressing infects the wheat seed of fusarium graminearum.Every diameter 20cm basin alms bowl 25 seeds of sowing, each process is repeated 10 basins, is placed in hot-house culture.Emerge latter 10 days and check emergence rates and death rate, calculate and gibberellic hypha is caused bud-rot and the withered prevention effect of Seedling.Retain 10 young plants that growing way is consistent simultaneously, and inoculation contains the wheat bran medium of rhizoctonia cerealis (Rhizoctoniacerealis) in substrate, with to blade inoculation Powdery Mildew and leaf rust spore, inoculate latter 14 days and respectively process investigation 100 strain wheat seedlings, calculate banded sclerotial blight, powdery mildew and the strain sickness rate of rust and prevention effect.Simultaneously according to single dose bud-rot microbial to head blight and the withered preventive effect of Seedling, by theoretical preventive effect (E=X+ (100-X) Y/100 of Abbott (1925) method calculation composition, wherein E is theoretical preventive effect, X is jingganmycin single dose preventive effect, Y is propiconazole single dose preventive effect) and coefficient of synergism (the actual preventive effect of compositions application is divided by theoretical preventive effect × 100), it the results are shown in Table 6.
Table 6: disinfectant use in agriculture suspending agent of the present invention processes effect and the synergy test result of wheat seed preventing and treating wheat diseases
* Abbott thinks that the actual preventive effect of compositions is potentiation (lower same) with the ratio of theoretical preventive effect more than 1 (or coefficient of synergism is more than 100).
Above-mentioned result of the test shows, disinfectant use in agriculture suspending agent Dressing of the present invention suffers from the wheat seed of head blight, it is possible not only to effectively prevent and treat the microbial bud-rot of head blight and Seedling is withered, and test dose process in the range of (10～1000gai jingganmycins and 2～15gai propiconazoles/100kg seed) all to preventing and treating, the microbial Seedling of head blight is withered fabulous potentiation, coefficient of synergism is far longer than 100, and potentiation is the most notable.Although jingganmycin single dose has certain preventive effect to banded sclerotial blight, but the most invalid to powdery mildew and rust；Propiconazole single dose then all has favorable effect, and the obvious potentiation of performance mixed with jingganmycin to head blight in seedling stage, banded sclerotial blight, powdery mildew and rust.
Disinfectant use in agriculture suspending agent the most of the present invention is prevented and treated the potentiation of wheat scab and reduces detoxifying function
According to jingganmycin and weight ratio 1:50 of propiconazole, 1:20,1:3,1:2,1:1.5,1:1,2:1,5:1,5:1.5,50:3,10:1,10:1.5,50:2.5,50:1.5 and 50:1, prepare the disinfectant use in agriculture suspending agent experiment sample of the present invention of content 20% (1:50,1:20,1:3,1:2,1:1.5,1:1,2:1,5:1,5:1.5) by weight and 40% (10:1,10:1.5,50:3,50:2.5,50:1.5,50:1) respectively.Sample preparation methods is after propiconazole and the former medicine of jingganmycin mix according to above-mentioned weight ratio, add in the solution containing 5% ethylene glycol by weight and mixed with propylene glycol solvent (volume ratio 1:1), 2% glycerol, 1.0%NNO-1,1.5%NNO-7 dispersant, 3% sodium lauryl sulphate and a small amount of water, finally it is supplemented to 100% with water, is crushed to pharmacy particle diameter 5 μm of 90% through sand mill.
Design every mu and use jingganmycin and the various dose of propiconazole compositions, devise corresponding single dose according to the single dose consumption used in disinfectant use in agriculture suspending agent of the present invention simultaneously and process.In April, 2013 carries out the experiment sieving of field control head blight on farm, lake, Baima, Jiangsu Province, and wheat breed is Huaihe River wheat 22, at Semen Tritici aestivi flowering initial stage each medicament sample, water is sprayed process, is spaced and within 5 days, sprays second time medicine, every mu of injection flow rate 50 kilograms.Each process repeats 3 communities, every plot area 50 square metres.Set every mu of use of 50% carbendazol wettable powder 80 grams to process as comparison medicament simultaneously.The correlation method that the antibacterial field control effectiveness test criterion industry standard issued according to the Ministry of Agriculture specifies, in milk stage investigation wheat scab, a situation arises, prevents and treats the actual effect of head blight according to each process, the potentiation of calculation composition.By theoretical preventive effect (E=X+ (100-X) Y/100 of Abbott (1925) method calculation composition, wherein E is theoretical preventive effect, X is jingganmycin single dose preventive effect, and Y is propiconazole single dose preventive effect) and coefficient of synergism (the actual preventive effect of compositions application is divided by theoretical preventive effect × 100).
Toxin determination method: respectively processing 5 sampling 200 wheat heads, indoor threshing in dough stage, after drying, 30 grams of wheat grain of randomization are pulverized.By Goswami and Kistler method, take 5 grams of flour to be placed in centrifuge tube, the acetonitrile of addition 20mL: water (84:16) extracting solution, shake 24 hours on shaking table after scroll machine mixing, it is centrifuged 10min with 5000rpm, take supernatant 2mL N air-blowing in Eppendorf centrifuge tube to do, preservation at temperature-20 DEG C.100 μ LTMS derivatization reagent (TMSI:TMCS=100:1) are added during detection, mixing 10min, adds 1mL ultra-pure water, concussion layering, Aspirate supernatant is added to GC sample injection bottle, carries out content of toxins detection by the gas chromatogram (GC-ECD) equipped with electronics capture monitor.
Wheat grain infection dosage assay method: take and be placed in 50mL centrifuge tube in the flour 2g of Mycotoxin identification, add 20%CTAB pathogenic bacteria DNA extraction buffer, add 50 μ L E.C. 3.4.21.64s and 30 μ LRNA enzymes, fully mixing, hatch 3 hours at temperature 65 DEG C, it is centrifuged 10min with 10000rpm, 20mL supernatant all goes in 50mL centrifuge tube, add equal-volume phenol: chloroform: isoamyl alcohol (25:24:1) acutely shakes, it is centrifuged 5min with 10000rpm, takes supernatant 10mL and add the 3molL of 1/10 cold volume-1Sodium acetate and 2 times of volume dehydrated alcohol, precipitate 24 hours at temperature-20 DEG C, is centrifuged the ethanol elution 2 times of 10min, precipitate 20mL70% with 10000rpm, adds 50 μ LTE buffer solution and dissolves, preserve this DNA profiling temperature-20 DEG C after drying.Primer is designed according to DON synthesis key gene Tri5, use the DNA in real-time quantitative PCR amplification sample, and calculating Tri5DNA content in every gram of wheat samples (μ gDNA/g Semen Tritici aestivi), calculation composition respectively processes the inhibitory action to thalline toxin synthesis capability.The results are shown in Table 7.
Table 7: disinfectant use in agriculture suspending agent of the present invention is prevented and treated the potentiation of wheat scab and reduces DON endotoxin contamination Experiment on Function result
Disinfectant use in agriculture suspending agent potentiation Field Screening of the present invention and result of the test show, disinfectant use in agriculture of the present invention sprayed to the pustulation period at the Semen Tritici aestivi flowering initial stage, when every mu of mixture using jingganmycin effective ingredient 1～150g and propiconazole effective ingredient 2～20 grams, the most all preventing and treatings to head blight have notable potentiation (coefficient of synergism is more than 100), and after jingganmycin and propiconazole combination application, the DON level of pollution reducing corn had notable potentiation, DON content more than 85% can be reduced, endotoxin contamination level is controlled the level of security below 1mgDON/kg corn.Also again showing that the pathogenic bacteria quantitative analysis results infecting wheat grain, jingganmycin has suppression thalline toxin synthesis capability, and propiconazole does not then have this effect simultaneously.Although carbendazim individual processing has the preventive effect of 67.5% to head blight, but sitotoxin level of pollution still has 4.4mg/kg corn, only declines 58.5% than blank.
Disinfectant use in agriculture suspending agent the most of the present invention is prevented and treated the potentiation of wheat scab and reduces detoxifying function
The present invention uses the test specimen preparation method of invention development test IV and V, devises the treatment dosage using disinfectant use in agriculture of the present invention further, devises single dose according to the consumption of jingganmycin and propiconazole in this disinfectant use in agriculture simultaneously and processes.Field test was carried out on Xin Yang farm, Jiangsu in 2013.At No. 22 Semen Tritici aestivi flowering initial stages of Huaihe River wheat, water is sprayed process, be spaced and within 5 days, spray second time medicine, every mu of injection flow rate 50 kilograms.Each process is repeated 3 times, every plot area 50 square metres.Set 50% carbendazim suspending agent every mu 80mL as comparison chemicals treatment simultaneously.The correlation method specified about antibacterial field control effectiveness test criterion industry standard according to the Ministry of Agriculture, a situation arises to investigate wheat scab, powdery mildew, rust and leaf blight in milk stage, calculate disease index, prevention effect and above-mentioned Abbott (1925) method and calculate the potentiation to head blight preventing and treating, toxins checking method is identical with the method for the studies above V, (the results are shown in Table 8).
Table 8: disinfectant use in agriculture suspending agent of the present invention preventing and treating wheat diseases and minimizing sitotoxin test for contamination result
Above-mentioned Field information result shows, disinfectant use in agriculture suspending agent of the present invention prevents and treats wheat scab every mu jingganmycin 1～100 grams of effective ingredient, propiconazole every mu use 3～15 grams of effective ingredient are not only to wheat scab, leaf blight, powdery mildew and rust have fabulous prevention effect, it is much better than conventional carbendazim (the most experimental field in pathogenic bacterium colony, carbendazim is showed drug-fast pathogenic bacteria and account for 37.7%), and to including that the microbial head blight of Drug resistance head blight has highly significant potentiation preventive and therapeutic effect, its result is similar with the field test results on farm, lake, Baima in the same year.Wherein not only little with the preventive effect difference of more high dose by the effect of 3～15 grams with jingganmycin 3～50 grams, propiconazole with every mu, DON endotoxin contamination can be controlled within safety range, and dosage is few, low cost, beneficially environmental conservation, benefit is more preferable.It is especially noted that the corn mass of 1000 kernel that all antibacterial process is obviously higher than comparison, especially jingganmycin and propiconazole compositions-treated, mass of 1000 kernel increases by more than 15%, and production-increasing function is more significantly.
The potentiation of disinfectant use in agriculture wettable powder the most of the present invention preventing and treating barley scab and the effect of minimizing endotoxin contamination
According to disinfectant use in agriculture suspending agent of the present invention in field control head blight and the double good result controlling other diseases, the present invention devises beneficially decrement and uses pesticide and reduce the deal ratio of cost, is prepared for the disinfectant use in agriculture wettable powder of different content jingganmycin and propiconazole.Its preparation method is: after mixing according to propiconazole and jingganmycin former medicine weight ratio, add by weight 30% attapulgite carrier, 1.0%NNO-1 dispersant, 3% pull open powder wetting agent, 2% dodecylbenzene sodium sulfonate surfactant, last packing precipitated calcium carbonate, to 100%, is pulverized through sand mill and crosses 300 mesh sieves.Preparing content respectively is 20% (1:3,1:2,1:1.5,1:1) and the composite wettable powder sample of 60% (5:1,10:3,10:1).
Process and conventional many ketone wettable powder comparison medication by the single dose consumption in disinfectant use in agriculture, the single dose designing jingganmycin and propiconazole simultaneously by every mu.Within 2014, at the Fructus Hordei Vulgaris flowering initial stage, water is sprayed process, be spaced and within 5 days, spray second time medicine, every mu of injection flow rate 50 kilograms.Each process is repeated 3 times, every plot area 50 square metres.According to the correlation method of antibacterial field control effectiveness test principle states, a situation arises to investigate barley scab, powdery mildew, rust and leaf blight in milk stage, calculates disease index and and prevention effect.Simultaneously by research contents Abbott (1925) method, calculate the potentiation of mixture preventing and treating barley scab.Dough stage, often processes at 5 and takes each 150 fringes of the wheat head at random, takes back indoor threshing, detects and calculate wheat grain content of toxins by the method for research contents V.The impact of chemicals treatment dosage, preventive effect and potentiation and contratoxin content is shown in Table 9.
Table 9: barley diseases result of the test prevented and treated by disinfectant use in agriculture wettable powder of the present invention
Above-mentioned Field information shows, propiconazole single dose processes all has favorable effect to barley scab, leaf blight, powdery mildew and rust, and jingganmycin single dose is in addition to having relatively low preventive effect to head blight, and other diseases are not had effect substantially.But, when by compositions containing jingganmycin 3～50gai and propiconazole 3～15gai, increasing substantially the preventive effect of head blight, leaf blight, powdery mildew and rust, prevention effect reaches 80%～more than 90%, being better than the effect of the conventional many ketone of medicament of comparison, potentiation is notable.Especially with every mu with jingganmycin effective ingredient 3～50 grams, propiconazole with 3～15 grams, the consumption of propiconazole chemical bactericide can be greatly lowered, alleviate the ambient pressure of pesticide, reduce the endotoxin contamination of wheat grain.
Disinfectant use in agriculture wettable powder bucket the most of the present invention mixes preventing and treating wheat diseases and reduces the effect of endotoxin contamination
Use Zhejiang Tonglu Huifeng Bio-Chemical Co., Ltd. 64% jinggangmycin A soluble powder commercially and entrust 20% propiconazole wettable powder of south, Nanjing agriculture pesticide development in science and technology company limited processing.Use effective ingredient dosage according to the jingganmycin required for experimental design and propiconazole, weigh two kinds of medicaments respectively, add another kind of medicament, stirring after first half water being diluted one medicament, add second half water, spraying after mixing.Spray for the first time medicine at the Semen Tritici aestivi flowering initial stage, be spaced and within 5 days, spray medicine for the second time, every mu of injection flow rate 50 kilograms.Testing and carry out on Xin Yang farm, Jiangsu Province in April, 2014, wheat breed Huaihe River wheat 33, each process is repeated 3 times, every plot area 50 square metres.If many ketone suspending agent is comparison chemicals treatment.According to the correlation method of antibacterial field control effectiveness test principle states, a situation arises to investigate wheat scab, powdery mildew, rust and leaf blight in milk stage, calculates disease index and and prevention effect.Potentiation and Mycotoxin identification are identical with the studies above, and treatment dosage and preventive effect are shown in Table 10.
Table 10: jingganmycin and propiconazole wettable powder bucket mix the effect of preventing and treating wheat diseases
The studies above result shows, jingganmycin single dose and propiconazole single dose spray after when application, interim bucket is mixed, every mu with jingganmycin 3～100 grams of effective ingredient, propiconazole every mu use 3～15 grams of effective ingredient respectively to process the effect preventing and treating head blight and the mixture the being prepared as application effect on Wheat And Barley suitable, and wheat scab, powdery mildew, rust and leaf blight are also had obvious synergistic function, prevention effect is better than many ketone.Particularly wheat scab is had significant synergies, and similar with other sample results, and in the range of showing test dose, with propiconazole decrease in dose, potentiation strengthens.Wherein, every mu uses jingganmycin 3～50 grams and propiconazole 3～15 grams of effective ingredient all to can reach to prevent and treat head blight and double control leaf blight, powdery mildew, rust and the desired result of minimizing DON endotoxin contamination.
In a word, gibberella zeaze petch of wheat and barley described in the present invention is wheat scab and barley scab, the Fusarium pathogenic fungi (Fusariumspp.) including carbendazim resistance Fusarium spp. the wheat crops bud-rot that causes, Seedling is withered, fringe is rotten or withered fringe.
The studies above result shows, every mu uses containing 1～150 gram of jingganmycin and the compositions of 1～20 gram of propiconazole effective ingredient, before Wheat Sowing, seed treatment (coating or seed dressing) preventing and treating gibberella zeaze petch of wheat and barley has a notable potentiation, the most double controls powdery mildew of cereals, rust and banded sclerotial blight；Spraying water to the pustulation period in Semen Tritici aestivi heading, flowering, not only the preventing and treating to Wheat And Barley head blight has potentiation, but also has reduction DON endotoxin contamination level, increases mass of 1000 kernel and the double effect controlling powdery mildew, rust and leaf blight.
[beneficial effect]
The invention has the beneficial effects as follows:
Compared with conventional sterilization agent, the microbicide compositions of the present invention has the advantage that 1, compared with application single dose, said composition is used for preventing and treating gibberella zeaze petch of wheat and barley and has obvious potentiation, is remarkably improved the effect preventing and treating head blight, improves the ability of mankind's prevention and control wheat crops head blight；2, the level of pollution of corn DON toxin can be significantly reduced；3, dosage can be reduced, reduce cost, minimizing environmental pollution and pesticide residues；4, reduce antibacterial and select pressure, the Wheat Pathogenic Fungi Drug resistance to propiconazole can be delayed, and the gibberella zeaze petch of wheat and barley that carbendazim is developed immunity to drugs can be prevented and treated；5, there is the lasting period of prolongation jingganmycin, reduce times for spraying, double powdery mildew of cereals, rust, leaf blight and the banded sclerotial blight controlled, the advantage reducing the recruitment cost of Pesticide use；6, jingganmycin is the non-pollution microbial source pesticide that selectivity is strong, mixed with propiconazole, considerably reduces the using dosage of chemical pesticide, environmentally friendly.
[detailed description of the invention]
The present invention is will be better understood that by following embodiment.
The preventing and treating wheat diseases test of embodiment 1:42% disinfectant use in agriculture of the present invention suspending agent
Test period and place: in April, 2015, Xin Yang farm, Jiangsu
Test material: Semen Tritici aestivi, kind: Huaihe River wheat No. 33.
Test method:
Sample preparation methods: propiconazole medicine former with jingganmycin mixes according to weight ratio 1:20,1:6,5:16,5:2 and 6:1, the research V method described according still further to present specification, processing obtains the composition suspending agent test specimen of 42% different ratio.Every mu uses 25 milliliters, 50 milliliters, 100 milliliters of composite preparations.The Semen Tritici aestivi flowering initial stage is watered and sprays, and is spaced and within 5 days, sprays second time medicine, every mu of injection flow rate 50 kilograms.The corresponding dosage and the spray clear water that use wettable powder single dose are comparison simultaneously, and carbendazim is as comparison medicament, and each process is repeated 2 times, every plot area 100 square metres.
Correlation method according to antibacterial field control effectiveness test principle states, under in milk stage investigation wheat scab, powdery mildew, rust and leaf blight, a situation arises, calculate disease index and and prevention effect and to the potentiation of head blight and the effect of contratoxin Environmental capacity, the results are shown in Table 11 for it.
Table 11:42% disinfectant use in agriculture of the present invention suspending agent preventing and treating wheat diseases result of the test
Above-mentioned Field information result shows, uses 0.5～36g jingganmycin and 3～20 grams of propiconazoles to have good wheat diseases prevention effect.Preventing and treating the coefficient of synergism 120～162 of head blight, potentiation is notable.But, every mu of 3～15 grams of jingganmycins of application and during 3～16 grams of propiconazoles, significantly reduce endotoxin contamination level, potentiation is extremely obvious, prevents and treats the effect of head blight all more than 85%, and the most double can control wheat powdery mildew, rust and leaf blight.These results are it is also shown that use 0.5～36 gram of jingganmycin and during 3～20 grams of propiconazole/mu, and the DON content of toxins in corn relatively compares decline more than 90%, and ratio is by jingganmycin or propiconazole individual processing, and attenuating effects is the most notable.
The preventing and treating wheat diseases test of embodiment 2:64% disinfectant use in agriculture of the present invention wettable powder
Test period and place: in April, 2015, the Along The Yangtze River In Jiangsu Province institute of agricultural sciences (Rugao)
Test material: Semen Tritici aestivi.Kind: raise wheat No. 4.
Test method: mix according to propiconazole and jingganmycin former medicine weight ratio 1:1, prepares 64% wettable powder test specimen according to the method for the research VII of present specification description.Every mu uses jingganmycin and propiconazole active constituent content 4～the composite wettable powder of 20 grams respectively.Raising No. 4 Semen Tritici aestivi flowering initial stages of wheat, water is sprayed, be spaced and within 5 days, spray second time medicine, every mu of injection flow rate 45 kilograms.Using 20% single dose wettable powder dosage in being used in mixed way and spray clear water is comparison, if the wettable powder of ketone more than 50% 60 grams/acre is comparison medicament.Each process is repeated 3 times, every plot area 50 square metres.According to the correlation method of antibacterial field control effectiveness test principle states, a situation arises to investigate wheat scab, powdery mildew, rust and leaf blight in milk stage, calculates disease index and and prevention effect.The results are shown in Table 12.
Table 12:64% disinfectant use in agriculture of the present invention wettable powder preventing and treating wheat diseases result of the test
Above-mentioned Field information result shows, each 4～20 grams of every mu of compositions effective content using jingganmycin and propiconazole 1:1 deal ratio all has good prevention effect to wheat scab, leaf blight, powdery mildew and rust, but to use jingganmycin and each 4～8 grams of effective ingredient best results of propiconazole.Compositions-treated can dramatically increase mass of 1000 kernel, and production-increasing function is obvious.
Embodiment 3:20% disinfectant use in agriculture of the present invention wettable powder preventing and treating wheat diseases and reduction endotoxin contamination test
Test period and place: in April, 2015, the Along The Yangtze River In Jiangsu Province institute of agricultural sciences (Rugao), test arrangement is in the identical field of example 2.
Test material: Semen Tritici aestivi.Kind: raise wheat No. 4.
Test method: after mixing according to propiconazole and jingganmycin former medicine weight ratio 1:1, add 10% kaolin support, 2% sodium lignin sulfonate auxiliary agent, 2% carboxymethyl cellulose, 1% alkylphenol-polyethenoxy base ether phosphate and 2% polyvinyl alcohol by weight, finally interpolation precipitated calcium carbonate carrier is to 100%, pulverizes through sand mill and crosses 300 mesh sieves.Prepare the composite wettable powder experiment sample that content is 20%.Every mu uses jingganmycin and the composite wettable powder of propiconazole active constituent content 20,15,6 grams respectively.Raising No. 4 Semen Tritici aestivi flowering initial stages of wheat, water is sprayed, be spaced and within 5 days, spray second time medicine, every mu of injection flow rate 45 kilograms.Using 20% single dose wettable powder dosage in being used in mixed way and spray clear water is comparison, if the wettable powder of ketone more than 50% 60 grams/acre is comparison medicament.Each process is repeated 3 times, every plot area 50 square metres.According to the correlation method of antibacterial field control effectiveness test principle states, a situation arises to investigate wheat scab, powdery mildew, rust and leaf blight in milk stage, calculates disease index and and prevention effect.Result of the test is shown in Table 13.
Table 13:20% disinfectant use in agriculture of the present invention wettable powder preventing and treating wheat diseases result of the test
Above-mentioned result of the test shows, disinfectant use in agriculture of the present invention prevents and treats the potentiation of head blight and production-increasing function is notable.This effect is relevant with using dosage, and does not has significance relation with content and the auxiliary agent of process preparation.
Embodiment 4:30% disinfectant use in agriculture of the present invention water dispersible granules preventing and treating wheat diseases and reduction endotoxin contamination test
Test period and place: in April, 2015, farm, lake, Baima, Jiangsu
Test material: Semen Tritici aestivi.Kind: China wheat No. 4.
Test method: obtain 30% disinfectant use in agriculture water dispersible granules of the present invention with the processing of propiconazole weight ratio 5:1 according to jingganmycin.Its processing method is as follows: 39.1 weight portion 64% jingganmycins (effective ingredient 25 parts), 5.4 weight portion 92% propiconazoles (effective ingredient 5 parts), 30 parts sulfuric acid ammoniums, 17.2 weight portion precipitated calcium carbonates, 2.5 parts of alkylphenol polyoxyethylene, 2.5 parts of dodecylbenzene sodium sulfonate and 3.5 parts of polyoxyethylene carboxylates, pulverize, sieve, pelletize.
400,200,100,60 grams of described disinfectant use in agriculture water dispersible granules of every mu of use, sprayed water at the Semen Tritici aestivi flowering initial stage, were spaced and within 5 days, sprayed second time medicine, every mu of injection flow rate 50 kilograms.Using 20% single dose wettable powder 100 grams/acre and spray clear water is comparison, and each process is repeated 3 times, every plot area 50 square metres.According to the correlation method of antibacterial field control effectiveness test principle states, a situation arises to investigate wheat scab, powdery mildew, rust and leaf blight in milk stage, calculates disease index and and prevention effect.Result of the test is shown in Table 14.
Table 14:30% disinfectant use in agriculture of the present invention water dispersible granules preventing and treating wheat diseases result of the test
Above-mentioned Field information effect shows, every mu is sprayed by 18～120 grams of effective ingredient of 5 parts of jingganmycins and the compositions water dispersible granules of 1 part of propiconazole, wheat scab, powdery mildew, rust and leaf blight all there is good prevention effect, especially when spraying compositions effective ingredient 18～60 grams for every mu, cost performance is best.
The preventing and treating wheat diseases test of embodiment 5:32% disinfectant use in agriculture of the present invention aqueous emulsion
Test period and place: in April, 2015, farm, lake, Baima, Jiangsu
Test material: Semen Tritici aestivi.Kind: China wheat No. 4.
Test method: 32% disinfectant use in agriculture method for producing aqueous emulsion of the present invention is as follows: former medicine propiconazole is made suspending agent, adds ethylene oxide-propylene oxide block copolymer nonionic emulsifier and stirs into homogeneous oil phase；Additionally jingganmycin aqueous solution, ethylene glycol antifreeze and 1% benzoic acid are mixed into aqueous phase.Under high velocity agitation described aqueous phase is gradually added in above-mentioned oil phase, prepares the oil-in-water type aqueous emulsion of 32% jingganmycin and propiconazole 1:3.
Water, by compositions aqueous emulsion 100,50,25,12.5 ml of formulation, is sprayed by every mu at the Semen Tritici aestivi flowering initial stage, is spaced and within 5 days, sprays second time medicine, every mu of injection flow rate 50 kilograms.Using 20% single dose wettable powder 100 grams/acre and spray clear water is comparison, and each process is repeated 3 times, every plot area 50 square metres.According to the correlation method of antibacterial field control effectiveness test principle states, a situation arises to investigate wheat scab, powdery mildew, rust and leaf blight in milk stage, calculates disease index and and prevention effect.Result of the test is listed in Table 15 below.
Table 15:32% disinfectant use in agriculture of the present invention aqueous emulsion preventing and treating wheat diseases result of the test
Above-mentioned Field information result shows, every mu uses disinfectant use in agriculture effective ingredient 8 of the present invention～32 grams all to have preferable prevention effect to wheat scab, powdery mildew, rust and leaf blight, and every mu uses compositions effective ingredient 4 grams also to have the notable effect preventing and treating these wheat diseases.
Embodiment 6:45% disinfectant use in agriculture of the present invention wettable powder preventing and treating wheat diseases and reduction endotoxin contamination test
Test period and place: in April, 2015, the Along The Yangtze River In Jiangsu Province institute of agricultural sciences (Rugao)
Test material: Semen Tritici aestivi.Kind: raise wheat No. 4.
Test method: prepare jingganmycin and the 45% disinfectant use in agriculture wettable powder test specimen of the present invention that propiconazole weight ratio is 2:1 according to the method for the research VII of present specification description, entrust the institute of agricultural sciences of the area along the Yangtze River in Jiangsu Province to carry out field test.Every mu uses 50,40,30 and 20 grams of described wettable powder formulation respectively.At the Semen Tritici aestivi flowering initial stage, water is sprayed, be spaced and within 5 days, spray second time medicine, every mu of injection flow rate 50 kilograms.Using 20% jingganmycin and propiconazole single dose wettable powder, compare chemicals treatment by the single dose used in mixture and 40 grams of carbendazim effective ingredient, and to spray clear water be blank, each process is repeated 3 times, every plot area 50 square metres.According to the correlation method of antibacterial field control effectiveness test principle states, in milk stage investigation wheat scab and powdery mildew, a situation arises, calculates disease index and and prevention effect.
Respectively process 5 sampling totally 200 fringes during results, sample send after threshing Agricultural University Of Nanjing detect content of toxins.Toxins checking method is described above.Result of the test is shown in Table 16.
Table 16:45% disinfectant use in agriculture of the present invention wettable powder preventing and treating wheat diseases result of the test
Can be seen that from the experimental result of above-mentioned Field information embodiment 1～6, based on finding that jingganmycin can suppress the gibberellic hypha virulence factor biosynthetic effect of DON toxin, using disinfectant use in agriculture of the present invention process wheat seeds and spray in field, the preventing and treating to wheat scab has significant synergistic function.Meanwhile, wheat crops powdery mildew, rust, leaf blight and banded sclerotial blight also there is preferable prevention effect.In addition, inventor uses jingganmycin that some of them combine and propiconazole compositions to carry out 3 years experiment and demonstrations on the Along The Yangtze River In Jiangsu Province institute of agricultural sciences, Xin Yang farm, Jiangsu and farm, lake, Baima and other places, all it is demonstrated by Wheat And Barley head blight is had fine preventive effect, and the most double controls powdery mildew, leaf blight and rust.The present invention not only solves the fusarium graminearum that China faces at present and is difficult to the great difficult problem of prevention and control because of carbendazim resistance, and can overcome the food-safety problem that DON endotoxin contamination Semen Tritici aestivi caused.Compared with antibacterial conventional in prior art, disinfectant use in agriculture of the present invention can be substantially reduced the pollution to corn of the Fusarium spp. DON toxin, also as have synergistic function, chemical pesticide consumption and drug cost can be greatly reduced, and for reducing environmental pollution, improvement Drug resistance wheat and barley fungal disease, ensuring food safety has good application prospect.

Claims (10)

1. the composition pesticide being used for preventing and treating gibberella zeaze petch of wheat and barley, it is characterised in that described composition pesticide is made up of according to weight ratio 1～150:1～20 with propiconazole jingganmycin.
Composition pesticide the most according to claim 1, it is characterised in that described composition pesticide is made up of according to weight ratio 5～65:3～18 with propiconazole jingganmycin.
Composition pesticide the most according to claim 1, it is characterised in that described composition pesticide is made up of according to weight ratio 10～20:6～16 with propiconazole jingganmycin.
4. one kind for preventing and treating the disinfectant use in agriculture of gibberella zeaze petch of wheat and barley, it is characterised in that it contains 0.1～90.0% composition pesticide described in any claim in claim 1-3 by weight, and surplus is acceptable carrier and/or auxiliary agent in pesticide.
Disinfectant use in agriculture the most according to claim 4, it is characterised in that it contains 10～75.0% composition pesticide described in any claim in claim 1-3 by weight, and surplus is acceptable carrier and/or auxiliary agent in pesticide.
Disinfectant use in agriculture the most according to claim 4, it is characterised in that it contains 26～58.0% composition pesticide described in any claim in claim 1-3 by weight, and surplus is acceptable carrier and/or auxiliary agent in pesticide.
7. according to the disinfectant use in agriculture described in claim any one of claim 4-7, it is characterised in that it is suspending agent, aqueous emulsion, microemulsion, wettable powder or water dispersion granule.
8. according to the disinfectant use in agriculture described in claim any one of claim 4-7, it is characterised in that described carrier is that one or more are selected from water, attapulgite, Kaolin or precipitated calcium carbonate.
9. according to the disinfectant use in agriculture described in claim any one of claim 4-7, it is characterised in that described auxiliary agent is selected from ethanol, methanol, ethylene glycol, propylene glycol, NNO-1, NNO-7, xanthan gum, Polyethylene Glycol, glycerol, pulls open powder, sodium lauryl sulphate, dodecylbenzene sodium sulfonate, ammonium sulfate, alkylphenol polyoxyethylene, alkylphenol-polyethenoxy base ether phosphate, polyoxyethylene carboxylate, benzoic acid, sodium lignin sulfonate, carboxymethyl cellulose or polyvinyl alcohol.
10. according to the disinfectant use in agriculture described in claim 1 or 4, it is characterized in that described gibberella zeaze petch of wheat and barley is wheat scab and barley scab, the Fusarium pathogenic fungi (Fusariumspp.) including carbendazim resistance Fusarium spp. the wheat crops bud-rot that causes, Seedling is withered, fringe is rotten or withered fringe.
CN201610244977.7A 2016-04-19 2016-04-19 It is a kind of to be used to prevent composition pesticide containing propiconazole of gibberella zeaze petch of wheat and barley and application thereof Active CN105815321B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610244977.7A CN105815321B (en) 2016-04-19 2016-04-19 It is a kind of to be used to prevent composition pesticide containing propiconazole of gibberella zeaze petch of wheat and barley and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610244977.7A CN105815321B (en) 2016-04-19 2016-04-19 It is a kind of to be used to prevent composition pesticide containing propiconazole of gibberella zeaze petch of wheat and barley and application thereof

Publications (2)

Publication Number Publication Date
CN105815321A true CN105815321A (en) 2016-08-03
CN105815321B CN105815321B (en) 2018-06-22

Family

ID=56527276

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610244977.7A Active CN105815321B (en) 2016-04-19 2016-04-19 It is a kind of to be used to prevent composition pesticide containing propiconazole of gibberella zeaze petch of wheat and barley and application thereof

Country Status (1)

Country Link
CN (1) CN105815321B (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190000082A1 (en) * 2015-11-19 2019-01-03 Nanjing Agricultural University Tebuconazole-containing pesticide composition for controlling fusarium head blightand application thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006108552A2 (en) * 2005-04-08 2006-10-19 Syngenta Participations Ag Pesticidal mixtures
CN101632363A (en) * 2009-08-19 2010-01-27 南京南农农药科技有限公司 Pesticide combination containing propiconazole and validamycin A
CN103891714A (en) * 2014-03-26 2014-07-02 浙江钱江生物化学股份有限公司 Method for producing wettable powder through compound of validamycin A and propiconazole

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006108552A2 (en) * 2005-04-08 2006-10-19 Syngenta Participations Ag Pesticidal mixtures
CN101632363A (en) * 2009-08-19 2010-01-27 南京南农农药科技有限公司 Pesticide combination containing propiconazole and validamycin A
CN103891714A (en) * 2014-03-26 2014-07-02 浙江钱江生物化学股份有限公司 Method for producing wettable powder through compound of validamycin A and propiconazole

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20190000082A1 (en) * 2015-11-19 2019-01-03 Nanjing Agricultural University Tebuconazole-containing pesticide composition for controlling fusarium head blightand application thereof
US10588316B2 (en) * 2015-11-19 2020-03-17 Nanjing Agricultural University Tebuconazole-containing pesticide composition for controlling fusarium head blight and application thereof

Also Published As

Publication number Publication date
CN105815321B (en) 2018-06-22

Similar Documents

Publication Publication Date Title
CN105284821B (en) Metconazole-containing pesticide composition for preventing and treating wheat head blight and use thereof
CN105284822B (en) A kind of composition pesticide containing Tebuconazole for preventing and treating gibberella zeaze petch of wheat and barley and application thereof
CN102763657A (en) Germicide composite with synergistic effect
CN105707083B (en) A kind of purposes of effects enhancing and dose reducing composition pesticide in gibberella zeaze petch of wheat and barley is prevented and treated
CN105961402B (en) A kind of bactericidal composition and its application of jinggangmeisu and triticonazole
CN101658171A (en) Pesticide composition and application thereof
CN101669524B (en) Bactericide composition with tetraconazole
CN101653131B (en) Bactericidal composition containing azoxystrobin
CN107646859A (en) A kind of purposes of effects enhancing and dose reducing biology-chemical pesticide composition in gibberella zeaze petch of wheat and barley is prevented and treated
CN105815321B (en) It is a kind of to be used to prevent composition pesticide containing propiconazole of gibberella zeaze petch of wheat and barley and application thereof
CN105766937B (en) The bactericidal composition and its application of a kind of jinggangmeisu and kind bacterium azoles
CN105724394B (en) It is a kind of to be used to prevent and treat composition pesticide containing epoxiconazole of gibberella zeaze petch of wheat and barley and application thereof
CN105248427B (en) It is a kind of to be used to prevent and treat composition containing triadimefon pesticide of gibberella zeaze petch of wheat and barley and application thereof
CN105707082B (en) A kind of purposes of composition pesticide in gibberella zeaze petch of wheat and barley is prevented and treated
CN105685058B (en) A kind of composition pesticide containing nitrile bacterium azoles for preventing and treating gibberella zeaze petch of wheat and barley and application thereof
CN107333792A (en) By rosickyite azoles and the bactericide of the preventing and treating rice blast of miaow chaff azoles compounding
CN105660647B (en) It is a kind of to be used to prevent composition pesticide containing Prochloraz of gibberella zeaze petch of wheat and barley and application thereof
CN108849923A (en) A kind of biological composite bactericide for preventing and treating wheat scab
CN106962345A (en) A kind of bactericidal composition of 2-cyano-3-amino-3-phenylancryic acetate-containing and metrafenone
CN107535509A (en) It is a kind of to be used to prevent and treat composition pesticide of gibberella zeaze petch of wheat and barley and application thereof
CN107980790A (en) A kind of agricultural bactericidal composition containing metrafenone and Changchuan mycin
CN107980779A (en) A kind of bactericidal composition containing ZEN 90160 and metrafenone
CN108041057A (en) Prevent complex composition and its application of fruit diseases
CN105340902A (en) Sterilization composition containing benzovindiflupyr and epoxiconazole

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant