CN107980779A - A kind of bactericidal composition containing ZEN 90160 and metrafenone - Google Patents

Abstract

The present invention relates to a kind of bactericidal composition containing ZEN 90160 and metrafenone, active ingredient ZEN 90160 and the preferable weight ratio of metrafenone are 1 40:40 1, it is still more preferably 1 20:20 1, the quality parts ratio 1%~85% of active ingredient in the formulation, remaining is the auxiliary element for allowing to use and receiving in pesticide, the formulation of bactericidal composition of the present invention is water dispersible granules, wettable powder, suspending agent, aqueous emulsion, microemulsion etc., is mainly used for preventing wheat scab and wheat powdery mildew.

Description

A kind of bactericidal composition containing ZEN 90160 and metrafenone

Technical field：

The invention belongs to field of agricultural fungicide, is specifically related to a kind of bactericidal composition containing ZEN 90160 and metrafenone And its for preventing the application of wheat diseases.

Background technology：

ZEN 90160 is systemic fungicide, controlling object wide spectrum, and the foliage disease such as leaf for being mainly used for preventing wheat class is withered Disease, leaf rust, glume blight, brown spot, powdery mildew etc., compared with other methoxy acrylic bactericides, ZEN 90160 pair Wheat leaf blessing disease, net blotch and moire disease have stronger therapeutic effect.

Metrafenone is the Benzophenones fungicide of BASF AG's exploitation, is mainly used for preventing a variety of diseases of Ascomycotina Evil, especially has special efficacy to crop powdery mildew, plays the role of protection to all kinds of powdery mildews, treats, roots out and suppress production spore.

Wheat is most important cereal raise crop, and one of staple food crop of China in the world.Cereal crops The threat of the fungal diseases such as head blight, powdery mildew is usually faced, mainly chemical pesticide control powdery mildew is used in production, it is main at present Rely on benzimidazole, triazole type, methoxy acrylic bactericide.However, the fungicide of long-time service single kind Disease can be made to produce different degrees of resistance, prevention effect reduces.At present, for the prevention for the disease for being also easy to produce resistance, mainly Method is the compounding developed between the novel pesticide component of existing pesticide species no interactions resistance or existing pesticide species, and the former Since required development cost is high, cycle length, and the speed that development rate is much produced less than the resistance to the action of a drug at present.Therefore, It is a kind of when carrying out mixture for the different pesticide species of the mechanism of action preferably to select, by reasonably compounding, can improve anti- Effect, reduces effective ingredient consumption, cost-effective, delays the drug-fast generation of pathogen, and then can mitigate or even avoid pesticide Pollution to food and environment.Present inventor has made intensive studies the recipe determination of ZEN 90160 and metrafenone, It was found that ZEN 90160 and metrafenone are compounded can prevent wheat scab and wheat white powder at the same time in certain scope Disease, and can significantly reduce with survival dose, times for spraying is reduced, production cost is reduced, there are significant economical, societal benefits.

The content of the invention：

A kind of bactericidal composition containing ZEN 90160 and metrafenone, it is characterised in that the bactericidal composition is with pyridine oxygen Bacterium ester and metrafenone are active ingredient, and the weight ratio of ZEN 90160 and metrafenone is 1-80:80-1, preferable weight ratio are 1- 40:40-1, is still more preferably 1-20:20-1, particularly 1:10-10:1；The cumulant of ZEN 90160 and metrafenone is institute The 1%-85% of composition total weight is stated, is preferably 20%-75%；Composition is used to prevent wheat scab, wheat powdery mildew Etc. disease；The conven-tional adjuvants allowed on composition and pesticidal preparations are configured to any one formulation allowed on pesticide, such as water Dispersible granule, wettable powder, suspending agent, aqueous emulsion, microemulsion etc..

Compared with prior art, composition pesticide of the invention has the beneficial effect that：

(1) after composition of the invention compounding, treatment and protective effect is had concurrently, wheat scab and wheat can be prevented at the same time Powdery mildew, significantly reduces with survival dose, reduction times for spraying, reduction production cost；

(2) the active ingredient mechanism of action is different in composition of the invention, while effectively prevention crop disease, It can delay the drug-fast generation of pathogen, be conducive to delay the service life of pesticide activity component；

(3) composition preparation process of the invention is simple, is adapted to promote and apply in actual production.

1st, example of formulations

The present invention is illustrated with the following example, but is not limited the scope of the invention.Active ingredient used herein Active compound be purchased in market.

Embodiment 1,61% ZEN 90160 metrafenone wettable powder (60:1)

ZEN 90160 60%, metrafenone 1%, fatty alcohol polyoxyethylene ether 5%, sodium lignin sulfonate 7%, detergent alkylate Sulfoacid calcium 5%, diatomite complements to 100%.ZEN 90160, metrafenone, wetting dispersing agent, filler are uniformly mixed, through air-flow After pulverizer crushes, stirring 30min was both obtained.

Embodiment 2,45% ZEN 90160 metrafenone water dispersible granules (1:8)

ZEN 90160 5%, metrafenone 40%, lignosulfonates 5%, neopelex 5%, sodium sulphate 10%, ammonium chloride 3%, kaolin complements to 100%.ZEN 90160, metrafenone, wetting dispersing agent, disintegrant, filler are mixed Uniformly, after airslide disintegrating mill crushes, 30min is stirred, through mediating, rear add in fluidized-bed granulation dryer is granulated, done It is dry both to obtain.

Embodiment 3,30% ZEN 90160 metrafenone suspending agent (1:2)

ZEN 90160 10%, metrafenone 20%, alkylphenol polyoxyethylene ether 7%, calcium dodecyl benzene sulfonate 8%, xanthan Glue 2%, ethylene glycol 1%, water complements to 100%.First by ZEN 90160, metrafenone, emulsifying agent in ball crusher abrading-ball 2.5 Hour, then it is pumped into cutter and adds antifreezing agent, the mixing of thickener, water, is uniformly mixed through high speed shear and both obtained.

Embodiment 4,30% ZEN 90160 metrafenone aqueous emulsion (2:1)

ZEN 90160 20%, metrafenone 10%, sorbitan fatty acid ester 10%, fatty alcohol polyoxyethylene ether 5%, N- Methyl pyrrolidone 15%, turpentine oil 8%, xanthans 1%, propane diols 1%, water complements to 100%.By ZEN 90160, benzene bacterium Ketone, auxiliary agent, solvent mixing, are dissolved into uniform oil phase；Part water, antifreezing agent are mixed into uniform water phase；Anti- Oil phase is added into water phase while answering the stirring of kettle high speed, cutter is opened and carries out high speed shear, and adds remaining water and supplies, Shearing about half an hour both obtained.

Embodiment 5,45% ZEN 90160 metrafenone granule (8:1)

ZEN 90160 40%, metrafenone 5%, sorbitan fatty acid ester 5%, calcium lignosulfonate 3%, dodecyl Sodium sulfonate 2%, pull open powder 4%, bentonite complements to 100%.ZEN 90160, metrafenone, auxiliary agent, filler are uniformly mixed, powder It is broken, add water to be stirred after soaking, then be granulated with screw extruder pelletizer, sieve after dry and both obtain.

Embodiment 6,61% ZEN 90160 metrafenone microemulsion (1:60)

ZEN 90160 1%, metrafenone 60%, n,N-Dimethylformamide 7%, cyclohexanone 18%, ethylene glycol 2%, benzene second Base phenol polyethenoxy ether 13%, agriculture breast 500#5%, silicone compound 0.5%, water complements to 100%.By ZEN 90160, benzene Bacterium ketone dissolves in a solvent, adds surfactant, is uniformly mixed obtained oil phase.Water-soluble component and water are mixed to prepare water Phase, oil phase is added in water phase, stirring while adding both to obtain.

2nd, toxicity measures

In order to prevent the wheat powdery mildew in agricultural production, we have carried out mutual compounding with ZEN 90160 and metrafenone Synergy is studied, and specific method is as follows.

Experiment germ is wheat powdery mildew (Erysipe graminis).The test medicine that active compound is configured to need, Test method refers to《The 4th part of farm-chemical indoor determination test rule fungicide：Prevent wheat powdery mildew experiment pot-culture method》 With《The 6th part of farm-chemical indoor determination test rule fungicide：The synergy measure of mixture》.Single dose and each Mixed Pharmacy Setting 5 various concentrations gradients, (on the basis of pilot study, bacteriostasis rate is set in the range of 5%~90% by geometric progression It is fixed).If blank control, 5 basins are often handled, per 10 plants of wheat seedlings of basin, are repeated 4 times.Liquid is uniformly sprayed in spare with spray-on process Wheat seedling on, naturally dry.24h after chemicals treatment, with spore suspension spray inoculation, natural air drying, then moves to constant temperature Room, is 20~25 DEG C in temperature, relative humidity is 70%~90% greenhouse.Treat that blank control disease leaf rate reaches more than 80% When, incidence is managed in classification investigation everywhere, the whole blades of often processing investigation.Stage division：

0 grade：Disease-free spot；

1 grade：Lesion area accounts for less than the 5% of whole leaf area；

3 grades：Lesion area accounts for the 6%~15% of whole leaf area；

5 grades：Lesion area accounts for the 16%~25% of whole leaf area；

7 grades：Lesion area accounts for the 26%~50% of whole leaf area

9 grades：Lesion area accounts for more than the 50% of whole leaf area.

According to survey data, the disease index and prevention effect of each processing are calculated.Pass through preventive effect niqueMin and series concentration To the linear regression analysis between numerical value, obtain the EC50 values of each medicament.The co-toxicity coefficient of mixture is calculated with the abundant methods of Sun Yun (CTC), activity of the reagent agent to germ is evaluated with this.The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergy work With；CTC≤80 show as antagonism；80 ＜ CTC ＜ 120 show as summation action.Measurement result is shown in Table 1.

Synergy measurement result of the composition of the present invention of table 1 to wheat powdery mildew

Synergy measurement result shows：ZEN 90160 and metrafenone are used with the ratio of table 1, have to wheat powdery mildew compared with High activity, synergistic effect is obvious, 1:During 2 ratio, synergistic effect is most obvious, and co-toxicity coefficient has reached 151.42.

3rd, field control effectiveness test

Test of pesticide effectiveness example 1：ZEN 90160 and metrafenone are to the field control effectiveness test of wheat scab and wheat powdery mildew-new Foreign farm

Test period and place：The 4-6 months in 2015, Jiangsu Xin Yang farms

Test material：Wheat, kind：Huaihe River wheat 33

Reagent agent：Embodiment 1-6,20% metrafenone wettable powder (comparison medicament 1), 25% chlorfenapyr suspending agent (comparison medicament 2), 80% carbendazol wettable powder (comparison medicament 3).

Spraying time and application method：Wheat flowering dispenser at initial stage, second of dispenser after 3-5 days, each 20 squares of cell Rice, each processing are repeated 3 times.

According to the correlation method of fungicide field control effectiveness test principle states, wheat scab, white powder are investigated in milk stage A situation arises for disease, calculates disease index and prevention effect, the results are shown in table 2.

2 test effect of table

Toxin determination method：5 points of 200 wheat heads of sampling, indoor threshing, 30 grams of grab sample after drying are respectively handled in dough stage Wheat crushes.By Goswami and Kistler methods, take 5 grams of flour to be placed in centrifuge tube, add the acetonitrile of 20mL：Water (84: 16) extracting solution, when concussion 24 is small on shaking table after scroll machine mixes, 5000rpm centrifugation 10min, take supernatant 2mL in Eppendorf - 20 DEG C of preservations are done in N air-blowings in centrifuge tube.100 μ LTMS derivatization reagents (TMSI are added during detection：TMCS=100：1), mix 1mL ultra-pure waters are added after 10min, Aspirate supernatant is added to GC sample injection bottles after concussion layering, with equipped with electronics capture monitor Gas-chromatography (GC-ECD) carries out content of toxins detection.

Wheat infection dosage assay method：Take and be placed in the flour 2g of Mycotoxin identification in 50mL centrifuge tubes, add 20% CTAB germ DNA extraction buffers, add 50 μ L Proteinase Ks and 30 μ L RNase, fully mix, 65 DEG C hatching 3 it is small when after, 10000rpm centrifuges 10min, and 20mL supernatants are all gone in 50mL centrifuge tubes, add isometric phenol：Chloroform：Isoamyl alcohol (25:24:1) acutely concussion, 10000rpm centrifugation 5min, takes supernatant 10mL to add the acetic acid of the 3mol L-1 of 1/10 cold volume The absolute ethyl alcohol of sodium and 2 times of volumes, when -20 DEG C of precipitations 24 are small, 10000rpm centrifugation 10min, the second of sediment 20mL70% Alcohol elutes 2 times, the dissolving of 500 μ L TE solution is added after drying, -20 DEG C preserve the DNA profiling.Key gene is synthesized according to DON Tri5 designs primer, expands the DNA in sample using real-time quantitative PCR, and calculate Tri5DNA contents (μ in every gram of wheat samples GDNA/g wheats), calculation composition respectively handles the inhibitory action to thalline toxin synthesis capability.It the results are shown in Table 3.

Table 3, field control effectiveness test result

The composition synergistic effect Field Screening and result of the test table of present invention prevention wheat scab and wheat powdery mildew Bright, ZEN 90160 and metrafenone composition spray at wheat flowering initial stage to pustulation period, can not only effectively prevent wheat scab, And wheat powdery mildew can be effectively prevented, compared with single dose, preventive effect dramatically increases, and compared with current common medicament carbendazim, prevents Effect dramatically increases.While ZEN 90160 and metrafenone composition for preventing and controlling wheat diseases, the DON toxin of cereal can be also significantly reduced Level of pollution, compared with single dose, DON toxin suppresses to significantly increase, and compared with comparison medicament carbendazim, can reduce DON contents More than 90%, by level of security of the horizontal control of endotoxin contamination below 1mg DON/kg cereal.Each medicament is equal during experiment Have no that poisoning produces, to crop production safety.

In conclusion the composition of the present invention is superior to single dose and right in the prevention and control of wheat scab and wheat powdery mildew According to medicament, prevention effect can be significantly improved, reduces times for spraying, reduces dosage, it is horizontal to crop safety, DON endotoxin contaminations Also it is significantly reduced, while can also reduces adverse effect of the medicament to environment, delay the generation of disease drug resistance, to wheat disease Collaboration, synergy, the decrement use of the whole Synthetical prevention of harmful green and pesticide have important practical significance.

Claims (6)

1. a kind of bactericidal composition containing ZEN 90160 and metrafenone, it is characterised in that the bactericidal composition is with pyridine oxygen bacterium Ester and metrafenone are active ingredient, and the weight ratio of ZEN 90160 and metrafenone is 1-80:80-1.

2. composition according to claim 1, it is characterised in that the weight ratio of ZEN 90160 and metrafenone is 1-40:40-1, more More preferably 1-20:20-1, particularly 1:10-10:1.

3. according to any compositions of claim 1-2, it is characterised in that the cumulant of ZEN 90160 and metrafenone is institute The 1%-85% of composition total weight is stated, is preferably 20%-75%.

4. according to claim 1-3, it is characterised in that the composition can be by ZEN 90160, metrafenone and pesticidal preparations The conven-tional adjuvants of upper permission are configured to any one formulation allowed on pesticide.

5. bactericidal composition according to claim 4, it is characterised in that the formulation is water dispersible granules, wettable powder, hangs Floating agent, aqueous emulsion, microemulsion etc..

6. the composition of claim 1-3 or the fungicide of claim 4-5, for preventing wheat scab and wheat white powder Disease.