CN107980790A - A kind of agricultural bactericidal composition containing metrafenone and Changchuan mycin - Google Patents
A kind of agricultural bactericidal composition containing metrafenone and Changchuan mycin Download PDFInfo
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- CN107980790A CN107980790A CN201711439968.4A CN201711439968A CN107980790A CN 107980790 A CN107980790 A CN 107980790A CN 201711439968 A CN201711439968 A CN 201711439968A CN 107980790 A CN107980790 A CN 107980790A
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- metrafenone
- bactericidal composition
- changchuan mycin
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N35/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical
- A01N35/04—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having two bonds to hetero atoms with at the most one bond to halogen, e.g. aldehyde radical containing aldehyde or keto groups, or thio analogues thereof, directly attached to an aromatic ring system, e.g. acetophenone; Derivatives thereof, e.g. acetals
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- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
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- General Health & Medical Sciences (AREA)
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- Pest Control & Pesticides (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of agricultural bactericidal composition containing metrafenone and Changchuan mycin, its active ingredient is metrafenone and Changchuan mycin, both ratios of weight and number are 1：80~80：1, it is preferably 1：40~40：1, accumulated quality accounts for the 1%~85% of bactericidal composition gross mass.The composition of the present invention has significant synergistic function, wide sterilization spectrum, the conventional pesticide preparation such as microemulsion, aqueous emulsion, suspending agent, wettable powder, water dispersible granules or granule is can be made into, the prevention available for powdery mildews such as prevention wheat, grape, cucumber.
Description
Technical field
The present invention relates to a kind of agriculture bactericide composition, and specifically one kind is effective using metrafenone and Changchuan mycin
The bactericidal composition of component, is mainly used for preventing wheat, grape and powdery mildew of cucumber, belongs to mixed pesticide technical field.
Background technology
Metrafenone (metrafenone), be BASF Aktiengesellschaft exploitation Benzophenones fungicide, CAS accession number：
220899-03-6.The mechanism of action of metrafenone is the development and formation by disturbing plant pathogenic fungi Spore adhesion spore, is suppressed
The sprouting of powdery mildew spore；Secondly, metrafenone makes the bacterium of germ by disturbing the foundation and formation of acute actin tissue
The formation of filament apical cell is interfered and suppresses, so as to hinder mycelial normal development and growth, suppresses and hinders
The infringement of Powdery Mildew, has the function that significantly to prevent and treat powdery mildew.
Changchuan mycin (ascomycin), is a kind of microbial source fungicide, has root systemic action, but in on-bladed
Conduction is inhaled, spore germination and mycelial growth to gray mold pathogen have inhibitory action.Laboratory test discovery, Changchuan mycin
Also have to the various crop such as corn southern leaf blight, rice blast, anthracnose, powdery mildew, sclerotiniose, downy mildew fungal disease good anti-
Control effect.
Wheat powdery mildew is the worldwide disease occurred extensively in Wheat Production, and mainly chemical pesticide control is used in production
Powdery mildew, relies primarily on benzimidazole, triazole type, methoxy acrylic bactericide at present.However, long-time service is single
The fungicide of species can make disease produce different degrees of resistance, and prevention effect reduces.
At present, for the prevention for the disease for being also easy to produce resistance, main method is that exploitation resists with existing pesticide species no interactions
Property novel pesticide component or existing pesticide species between compounding, and the former is since required development cost is high, cycle length, and
The speed that development rate is much produced less than the resistance to the action of a drug at present.Therefore, mixed for the different pesticide species of the mechanism of action
A kind of preferably selection of timing, by reasonably compounding, can improve preventive effect, reduce effective ingredient consumption, cost-effective, delay
The drug-fast generation of pathogen, and then can mitigate or even avoid pollution of the pesticide to food and environment.Present inventor
The recipe determination of Changchuan mycin and metrafenone is had made intensive studies, finds to be compounded Changchuan mycin and metrafenone one
Wheat scab and wheat powdery mildew can be prevented in fixed scope at the same time, and can be significantly reduced with survival dose, reduction medication
Number, reduces production cost, has significant economical, societal benefits.
The content of the invention
A kind of bactericidal composition containing Changchuan mycin and metrafenone, it is characterised in that the bactericidal composition is with long river
Mycin and metrafenone are active ingredient, and the weight ratio of Changchuan mycin and metrafenone is 1-80:80-1, preferable weight ratio are 1-
40:The cumulant of 40-1, Changchuan mycin and metrafenone is the 1%-85% of the composition total weight, and composition is used to prevent small
The diseases such as wheat head blight, wheat powdery mildew；The conven-tional adjuvants allowed on composition and pesticidal preparations are configured to what is allowed on pesticide
Any one formulation, such as water dispersible granules, wettable powder, suspending agent, aqueous emulsion, microemulsion etc..
Compared with prior art, composition pesticide of the invention has the beneficial effect that：
(1) after composition of the invention compounding, treatment and protective effect is had concurrently, wheat scab and wheat can be prevented at the same time
Powdery mildew, significantly reduces with survival dose, reduction times for spraying, reduction production cost；
(2) the active ingredient mechanism of action is different in composition of the invention, while effectively prevention crop disease,
It can delay the drug-fast generation of pathogen, be conducive to delay the service life of pesticide activity component；
(3) composition preparation process of the invention is simple, is adapted to promote and apply in actual production.
1st, example of formulations
The present invention is illustrated with the following example, but is not limited the scope of the invention.Active ingredient used herein
Active compound be purchased in market.
Embodiment 1,61% Changchuan mycin metrafenone wettable powder (60:1)
Changchuan mycin 60%, metrafenone 1%, fatty alcohol polyoxyethylene ether 5%, sodium lignin sulfonate 7%, detergent alkylate
Sulfoacid calcium 5%, diatomite complements to 100%.Changchuan mycin, metrafenone, wetting dispersing agent, filler are uniformly mixed, through air-flow
After pulverizer crushes, stirring 30min was both obtained.
Embodiment 2,45% Changchuan mycin metrafenone water dispersible granules (1:8)
Changchuan mycin 5%, metrafenone 40%, lignosulfonates 5%, neopelex 5%, sodium sulphate
10%, ammonium chloride 3%, kaolin complements to 100%.Changchuan mycin, metrafenone, wetting dispersing agent, disintegrant, filler are mixed
Uniformly, after airslide disintegrating mill crushes, 30min is stirred, through mediating, rear add in fluidized-bed granulation dryer is granulated, done
It is dry both to obtain.
Embodiment 3,30% Changchuan mycin metrafenone suspending agent (1:2)
Changchuan mycin 10%, metrafenone 20%, alkylphenol polyoxyethylene ether 7%, calcium dodecyl benzene sulfonate 8%, xanthan
Glue 2%, ethylene glycol 1%, water complements to 100%.First by Changchuan mycin, metrafenone, emulsifying agent in ball crusher abrading-ball 2.5
Hour, then it is pumped into cutter and adds antifreezing agent, the mixing of thickener, water, is uniformly mixed through high speed shear and both obtained.
Embodiment 4,30% Changchuan mycin metrafenone aqueous emulsion (2:1)
Changchuan mycin 20%, metrafenone 10%, sorbitan fatty acid ester 10%, fatty alcohol polyoxyethylene ether 5%, N-
Methyl pyrrolidone 15%, turpentine oil 8%, xanthans 1%, propane diols 1%, water complements to 100%.By Changchuan mycin, benzene bacterium
Ketone, auxiliary agent, solvent mixing, are dissolved into uniform oil phase；Part water, antifreezing agent are mixed into uniform water phase；Anti-
Oil phase is added into water phase while answering the stirring of kettle high speed, cutter is opened and carries out high speed shear, and adds remaining water and supplies,
Shearing about half an hour both obtained.
Embodiment 5,45% Changchuan mycin metrafenone granule (8:1)
Changchuan mycin 40%, metrafenone 5%, sorbitan fatty acid ester 5%, calcium lignosulfonate 3%, dodecyl
Sodium sulfonate 2%, pull open powder 4%, bentonite complements to 100%.Changchuan mycin, metrafenone, auxiliary agent, filler are uniformly mixed, powder
It is broken, add water to be stirred after soaking, then be granulated with screw extruder pelletizer, sieve after dry and both obtain.
Embodiment 6,61% Changchuan mycin metrafenone microemulsion (1:60)
Changchuan mycin 1%, metrafenone 60%, n,N-Dimethylformamide 7%, cyclohexanone 18%, ethylene glycol 2%, benzene second
Base phenol polyethenoxy ether 13%, agriculture breast 500#5%, silicone compound 0.5%, water complements to 100%.By Changchuan mycin, benzene
Bacterium ketone dissolves in a solvent, adds surfactant, is uniformly mixed obtained oil phase.Water-soluble component and water are mixed to prepare water
Phase, oil phase is added in water phase, stirring while adding both to obtain.
2nd, toxicity measures
In order to prevent the wheat powdery mildew in agricultural production, we have carried out mutual compounding with Changchuan mycin and metrafenone
Synergy is studied, and specific method is as follows.
Experiment germ is wheat powdery mildew (Erysipe graminis).The test medicine that active compound is configured to need,
Test method refers to《The 4th part of farm-chemical indoor determination test rule fungicide：Prevent wheat powdery mildew experiment pot-culture method》
With《The 6th part of farm-chemical indoor determination test rule fungicide：The synergy measure of mixture》.Single dose and each Mixed Pharmacy
Setting 5 various concentrations gradients, (on the basis of pilot study, bacteriostasis rate is set in the range of 5%~90% by geometric progression
It is fixed).If blank control, 5 basins are often handled, per 10 plants of wheat seedlings of basin, are repeated 4 times.Liquid is uniformly sprayed in spare with spray-on process
Wheat seedling on, naturally dry.24h after chemicals treatment, with spore suspension spray inoculation, natural air drying, then moves to constant temperature
Room, is 20~25 DEG C in temperature, relative humidity is 70%~90% greenhouse.Treat that blank control disease leaf rate reaches more than 80%
When, incidence is managed in classification investigation everywhere, the whole blades of often processing investigation.Stage division：
0 grade：Disease-free spot；
1 grade：Lesion area accounts for less than the 5% of whole leaf area；
3 grades：Lesion area accounts for the 6%~15% of whole leaf area；
5 grades：Lesion area accounts for the 16%~25% of whole leaf area；
7 grades：Lesion area accounts for the 26%~50% of whole leaf area
9 grades：Lesion area accounts for more than the 50% of whole leaf area.
According to survey data, the disease index and prevention effect of each processing are calculated.Pass through preventive effect niqueMin and series concentration
To the linear regression analysis between numerical value, obtain the EC50 values of each medicament.The co-toxicity coefficient of mixture is calculated with the abundant methods of Sun Yun
(CTC), activity of the reagent agent to germ is evaluated with this.The co-toxicity coefficient (CTC) >=120 of complex preparation shows as synergy work
With；CTC≤80 show as antagonism；80 ＜ CTC ＜ 120 show as summation action.Measurement result is shown in Table 1.
Synergy measurement result of the composition of the present invention of table 1 to wheat powdery mildew
| Medicament | The proportioning of active ingredient | EC50(mg/L) | Co-toxicity coefficient |
| Changchuan mycin | —— | 32.46 | —— |
| Metrafenone | —— | 1.24 | —— |
| Changchuan mycin：Metrafenone | 1:60 | 1.01 | 125.12 |
| Changchuan mycin：Metrafenone | 1:80 | 0.98 | 141.67 |
| Changchuan mycin：Metrafenone | 1:2 | 1.21 | 151.42 |
| Changchuan mycin：Metrafenone | 2:1 | 2.56 | 135.24 |
| Changchuan mycin：Metrafenone | 8:1 | 6.65 | 128.54 |
| Changchuan mycin：Metrafenone | 60:1 | 18.70 | 122.84 |
Synergy measurement result shows：Changchuan mycin and metrafenone are used with the ratio of table 1, have to wheat powdery mildew compared with
High activity, synergistic effect is obvious, 1:During 2 ratio, synergistic effect is most obvious, and co-toxicity coefficient has reached 151.42.
3rd, field control effectiveness test
Test of pesticide effectiveness example 1：Changchuan mycin and metrafenone are to the field control effectiveness test of wheat scab and wheat powdery mildew-new
Foreign farm
Test period and place：The 4-6 months in 2015, Jiangsu Xin Yang farms
Test material：Wheat, kind：Huaihe River wheat 33
Reagent agent：Embodiment 1-6,20% metrafenone wettable powder (comparison medicament 1), 25% Changchuan mycin suspending agent
(comparison medicament 2), 80% carbendazol wettable powder of comparison medicament.
Spraying time and application method：Wheat flowering dispenser at initial stage, second of dispenser after 3-5 days, each 20 squares of cell
Rice, each processing are repeated 3 times.
According to the correlation method of fungicide field control effectiveness test principle states, wheat scab, white powder are investigated in milk stage
A situation arises for disease, calculates disease index and prevention effect and the effect of contratoxin contamination control, the results are shown in table 1.
Toxin determination method：5 points of 200 wheat heads of sampling, indoor threshing, 30 grams of grab sample after drying are respectively handled in dough stage
Wheat crushes.By Goswami and Kistler methods, take 5 grams of flour to be placed in centrifuge tube, add the acetonitrile of 20mL：Water (84:
16) extracting solution, when concussion 24 is small on shaking table after scroll machine mixes, 5000rpm centrifugation 10min, take supernatant 2mL in Eppendorf
- 20 DEG C of preservations are done in N air-blowings in centrifuge tube.100 μ LTMS derivatization reagents (TMSI are added during detection：TMCS=100：1), mix
1mL ultra-pure waters are added after 10min, Aspirate supernatant is added to GC sample injection bottles after concussion layering, with equipped with electronics capture monitor
Gas-chromatography (GC-ECD) carries out content of toxins detection.
Wheat infection dosage assay method：Take and be placed in the flour 2g of Mycotoxin identification in 50mL centrifuge tubes, add 20%
CTAB germ DNA extraction buffers, add 50 μ L Proteinase Ks and 30 μ L RNase, fully mix, 65 DEG C hatching 3 it is small when after,
10000rpm centrifuges 10min, and 20mL supernatants are all gone in 50mL centrifuge tubes, add isometric phenol：Chloroform：Isoamyl alcohol
(25:24:1) acutely concussion, 10000rpm centrifugation 5min, takes supernatant 10mL to add the acetic acid of the 3mol L-1 of 1/10 cold volume
The absolute ethyl alcohol of sodium and 2 times of volumes, when -20 DEG C of precipitations 24 are small, 10000rpm centrifugation 10min, the second of sediment 20mL70%
Alcohol elutes 2 times, the dissolving of 500 μ L TE solution is added after drying, -20 DEG C preserve the DNA profiling.Key gene is synthesized according to DON
Tri5 designs primer, expands the DNA in sample using real-time quantitative PCR, and calculate Tri5DNA contents (μ in every gram of wheat samples
GDNA/g wheats), calculation composition respectively handles the inhibitory action to thalline toxin synthesis capability.It the results are shown in Table 2.
Table 2, field control effectiveness test result
The composition synergistic effect Field Screening and result of the test table of present invention prevention wheat scab and wheat powdery mildew
Bright, Changchuan mycin and metrafenone composition spray at wheat flowering initial stage to pustulation period, can not only effectively prevent wheat scab,
And wheat powdery mildew can be effectively prevented, compared with single dose, preventive effect dramatically increases, and compared with current common medicament carbendazim, prevents
Effect increase by more than 30%.While Changchuan mycin and metrafenone composition for preventing and controlling wheat diseases, the DON of cereal can be also significantly reduced
Endotoxin contamination is horizontal, and compared with single dose, DON toxin suppresses to significantly increase, and compared with comparison medicament carbendazim, can reduce DON and contain
Amount more than 90%, by level of security of the horizontal control of endotoxin contamination below 1mg DON/kg cereal.Each medicament during experiment
Poisoning generation is showed no, to crop production safety.The above results show that composition of the invention is in wheat scab and white wheat
Single dose and comparison medicament are superior in the prevention and control of powder disease, prevention effect can be significantly improved, reduces times for spraying, reduces dosage,
To crop safety, DON endotoxin contamination levels are also significantly reduced, while can also reduce adverse effect of the medicament to environment, prolong
The generation of slow disease drug resistance, collaboration, synergy, decrement use to the whole Synthetical prevention of green and pesticide of wheat diseases have
Important realistic meaning.
Claims (5)
- A kind of 1. agricultural bactericidal composition containing metrafenone and Changchuan mycin, it is characterised in that：Active ingredient for metrafenone and Changchuan mycin, the ratio of weight and number of the two are 1：80~80：1.
- 2. agricultural bactericidal composition according to claim 1, it is characterised in that：The matter of preferable metrafenone and Changchuan mycin Amount is than being 1：40~40：1.
- 3. agricultural bactericidal composition according to claim 1, it is characterised in that：Active ingredient metrafenone and Changchuan mycin Accumulated quality accounts for the 1%~85% of bactericidal composition gross mass.
- 4. the bactericidal composition according to claim 1 to claim 3, it is characterised in that：The formulation that can be made into is micro emulsion Agent, aqueous emulsion, suspending agent, wettable powder, water dispersible granules or granule etc..
- 5. the agricultural bactericidal composition described in claim 1, it is characterised in that：It is white to be mainly used for prevention wheat powdery mildew, grape Powder disease, powdery mildew of cucumber etc..
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| CN201711439968.4A CN107980790A (en) | 2017-12-27 | 2017-12-27 | A kind of agricultural bactericidal composition containing metrafenone and Changchuan mycin |
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| CN201711439968.4A CN107980790A (en) | 2017-12-27 | 2017-12-27 | A kind of agricultural bactericidal composition containing metrafenone and Changchuan mycin |
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