CN105795290B - Hericium erinaceus compounding beverage with alpha-amylase inhibitory activity and preparation method thereof - Google Patents
Hericium erinaceus compounding beverage with alpha-amylase inhibitory activity and preparation method thereof Download PDFInfo
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- CN105795290B CN105795290B CN201610183850.9A CN201610183850A CN105795290B CN 105795290 B CN105795290 B CN 105795290B CN 201610183850 A CN201610183850 A CN 201610183850A CN 105795290 B CN105795290 B CN 105795290B
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- hericium erinaceus
- alpha
- extract
- supernatant
- inhibitory activity
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Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/38—Other non-alcoholic beverages
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/60—Sweeteners
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
- A23L2/52—Adding ingredients
- A23L2/68—Acidifying substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Non-Alcoholic Beverages (AREA)
Abstract
The invention discloses a kind of, and the Hericium erinaceus with alpha-amylase inhibitory activity compounds drinks, its raw material is composed of the following parts by weight: 0.003~0.01 part, 0.05~0.5 part of leaf extract tomorrow, 0.003~0.01 part of silk gum peptide extract, 2~8 parts of sugar alcohol, 0.000~0.003 part of low calorie sweetener, 0.1~0.3 part of acid, 1~5 part of rose liquid distilled from honeysuckle flowers or lotus leaves, 88~96 parts of water.Also disclose a kind of method for preparing the above-mentioned Hericium erinaceus compounding drinks with alpha-amylase inhibitory activity.Hericium erinaceus compounding drinks of the invention have moistening skin beautifying, nourishing stomach and spleen, the multiple health-care effect for inhibiting alpha-amylase activity, improving the immunity of the human body, and the mouthfeel of the fragrance and Hericium erinaceus, Angelica Keiskei sweetness with rose, the nutritive value and sense organ flavor quality of beverage are improved, while being suitble to the special populations such as diabetes patient or overweight people edible.
Description
Technical field
The invention belongs to food technology field, it is related to a kind of compounding beverage and preparation method thereof, and in particular to one kind has
Hericium erinaceus compounding drinks of alpha-amylase inhibitory activity and preparation method thereof.
Background technique
Starch digestion needs three kinds of main alpha-glucosidases to participate in enteron aisle: pancreatic alpha-amylase (HPA) first is by starch
It is hydrolyzed into the oligosaccharides of short linear chain or branched chain, oligosaccharides is then by maltose-glucuroide (MGAM) and sucrose-isomaltase
(SI) it is hydrolyzed to glucose, is quickly glucose by Starch Hydrolysis, blood sugar level rises.For being with lasting hyperglycemia
The glycosuria patient of main biochemical feature, the fast hydrolyzing of alpha-amylase can aggravate the degree of Diabetes Mellitus, therefore, pass through
The activity of the alpha-amylase in enteron aisle is inhibited to control the main side that diabetic's blood sugar level is a kind of Diet Adjustment
Formula.Verified many natural products can hinder food by inhibiting and reducing the activity of alpha-amylase in enteron aisle and saliva
The hydrolysis and digestion of middle carbohydrate reach the intake for reducing human body to sugar, reduce blood-sugar content in blood of human body, thus
Play the effect to diabetes mellitus prevention and control.Therefore, developing has the natural products beverage of inhibitory activity new alpha-amylase
There is product the huge market demand and application and development to be worth.
Silk gum in silk not only has edible value as a kind of novel food protein resource, and has medicinal
Value, the present invention overcome the heavy characteristic of silk gum by the silk gum peptide extract that enzymatic hydrolysis obtains.
Hericium erinaceus is the edible mushroom of delicious flavour, and a kind of rich in unsaturated fatty acid, vitamin, protein, amino
The nutritious and healthy food of acid, polysaccharide.Water-soluble polysaccharide, which has, in Hericium erinaceus improves immunity, anti-aging, reducing blood lipid, hypoglycemic etc.
Physiological function.
Angelica Keiskei is a kind of cold-resistant perennial Chinese celery section herbaceous plant, originates in Japanese Pacific Coast Area, is rich in
Rare VB in chlorophyll abundant, minerals, amino acid, natural organic?? and natural plants12.It is main in Angelica Keiskei
Functional component flavone compound has lowering blood pressure and blood fat, anti-inflammatory, prevention diabetes and other effects.
Rose liquid distilled from honeysuckle flowers or lotus leaves is that a kind of fragrancing liquid obtained is extracted from rose, rich in a variety of aromatic substances and small point a variety of
Sub- substance has effects that fragrant qi-regulating, conditioning endocrine, moistening skin beautifying.Rose liquid distilled from honeysuckle flowers or lotus leaves is not only advanced food flavor,
It is top raw material of skin care articles.
Therefore, those skilled in the art be dedicated to developing it is a kind of it is low in calories, there is the obvious alpha-amylase activity, easily of inhibiting
Digest, improve the immunity of the human body, reducing blood lipid, hypoglycemic, moistening skin beautifying multiple health-care effect drinks new product formula
And preparation method.
Summary of the invention
In view of the above drawbacks of the prior art, the object of the present invention is to provide a kind of compounding drinks, with bright
It is aobvious to inhibit alpha-amylase activity, improve the immunity of the human body, reducing blood lipid, hypoglycemic, moistening skin beautifying multi-functional, and there is uniqueness
Faint scent flavor.
To achieve the above object, the present invention provides a kind of Hericium erinaceus for having alpha-amylase inhibitory activity to compound beverage.?
In better embodiment of the invention, this has the raw material of the Hericium erinaceus compounding beverage of alpha-amylase inhibitory activity by following parts by weight
Several groups are grouped as: 0.003~0.01 part of Hericium erinaceus extract, 0.05~0.5 part of leaf extract tomorrow, silk gum peptide extract
0.003~0.01 part, 2~8 parts of sugar alcohol, 0.000~0.003 part of low calorie sweetener, 0.1~0.3 part of acid, rose liquid distilled from honeysuckle flowers or lotus leaves
1~5 part, 88~96 parts of water.
Further, above-mentioned Hericium erinaceus extract includes at least water-soluble polysaccharide, minerals and the albumen in Hericium erinaceus
Matter.
Further, above-mentioned leaf extract tomorrow includes at least cumarin, polysaccharide, protein and the flavonoids in Angelica Keiskei
Substance.
Further, the relative molecular weight of above-mentioned silk gum peptide extract is less than 3000Da.
Further, above-mentioned rose liquid distilled from honeysuckle flowers or lotus leaves includes at least citronellol, geraniol, nerol, borneol and eugenol.
Further, above-mentioned sugar alcohol is selected from xylitol, maltitol and D-sorbite.
Further, above-mentioned low calorie sweetener is selected from Aspartame, stevioside and Sucralose.
Further, above-mentioned acid is selected from citric acid, malic acid and tartaric acid.
Another aspect of the present invention, which provides, a kind of prepares the above-mentioned Hericium erinaceus compounding beverage for having an alpha-amylase inhibitory activity
Method.In the specific embodiment of the present invention, method includes the following steps:
1) ingredient: weigh leaf extract tomorrow, Hericium erinaceus extract, silk gum peptide extract, sugar alcohol, low calorie sweetener,
Acid, rose liquid distilled from honeysuckle flowers or lotus leaves and water are simultaneously uniformly mixed, and obtain mixed solution one；
2) it is centrifuged: above-mentioned mixed solution one being centrifuged, supernatant is taken；Preferably, in 2000~4000r/min revolving speed
10~20min of lower centrifugation；
3) homogeneous: above-mentioned supernatant is subjected to homogeneous, obtains mixed solution two；Preferably, under 200~300Mpa pressure
5~15min of homogeneous；
4) it deaerates: above-mentioned mixed solution two is de-gassed；Preferably, deaerate 10~15min under 0.1Mpa vacuum degree；
5) it sterilizes: two ultrahigh-temperature instant of mixed solution after degassing is sterilized；Preferably, the ultrahigh-temperature instant, which is sterilized, is
It is sterilized 3~8 seconds at 130 DEG C~135 DEG C；
6) filling: the mixed solution two after ultrahigh-temperature instant is sterilized carries out filling, there is alpha-amylase inhibition described in acquisition
Active Hericium erinaceus compounds beverage；Preferably, it is carried out at 80~85 DEG C filling.
Further, above-mentioned Hericium erinaceus extract the preparation method comprises the following steps: the Hericium erinaceus of drying crushed 40~80 meshes；
The crude hedgehog hydnum mushroom powder of acquisition is uniformly mixed with water by 1:15~1:30 solid-liquid ratio, 1~3 is extracted in 75 DEG C~85 DEG C hot water
Hour, centrifugation obtains supernatant, and freeze-drying obtains hedgehog hydnum mushroom powder；Above-mentioned hedgehog hydnum mushroom powder and 80% ethyl alcohol are pressed into 1:10~1:
30 solid-liquid ratios are uniformly mixed, and stand 1~3 hour at -18 DEG C~-23 DEG C, collect sediment；Above-mentioned sediment is freeze-dried
Obtain above-mentioned Hericium erinaceus extract.
Preferably, above-mentioned sediment is redissolved with water, is reused 80% alcohol treatment 1~2 time, is then freeze-dried again.
Further, above-mentioned leaf extract tomorrow the preparation method comprises the following steps: clean fresh angelica keiskei clean, chopping；With water
It is uniformly mixed by 1:5~1:10 solid-liquid ratio, is extracted 0.5~1 hour in 50 DEG C~70 DEG C hot water, centrifugation obtains supernatant three；
Above-mentioned supernatant three is evaporated concentration；Solution after concentration is spray-dried, Angelica Keiskei powder is obtained；By above-mentioned tomorrow
75% ethyl alcohol of Ye Fenyu is uniformly mixed by 1:3~1:8 solid-liquid ratio, stands 1~2 hour at 50 DEG C~60 DEG C, in centrifugation acquisition
Clear liquid four；Above-mentioned supernatant four is subjected to freeze-drying and obtains above-mentioned leaf extract tomorrow.
Preferably, above-mentioned evaporation and concentration carries out in film-rising evaporator, and the feeding temperature of the film-rising evaporator is 51
DEG C~53 DEG C, drop temperature is 67 DEG C~69 DEG C, and raw vapor (steam) temperature is 106 DEG C~108 DEG C, and secondary steam temperature is 66 DEG C~68
DEG C, cooling water inlet temperature is 14 DEG C~16 DEG C, and cooling water outlet temperature is 29 DEG C~31 DEG C.
Preferably, above-mentioned spray drying be in 148~152 DEG C of inlet air temperature, 69~71 DEG C of leaving air temp, 498~
It is spray-dried under the charging rate of 502mL/h.
Further, above-mentioned silk gum peptide extract the preparation method comprises the following steps: the zymolyte of sericin peptide taken crossed molecular cut off being
The film of 3K obtains supernatant one；Above-mentioned supernatant one is evaporated concentration, obtains silk gum Gly-His-Lys；By above-mentioned silk gum Gly-His-Lys with
80% ethyl alcohol is uniformly mixed by 1:60~1:100 solid-liquid ratio, stands 2~3 hours at -18 DEG C~-22 DEG C, and centrifugation obtains supernatant
Two, above-mentioned supernatant two is subjected to freeze-drying and obtains above-mentioned silk gum peptide extract.
Preferably, above-mentioned evaporation and concentration carries out in film-rising evaporator, and the feeding temperature of the film-rising evaporator is 43
DEG C~45 DEG C, drop temperature is 40 DEG C~42 DEG C, and raw vapor (steam) temperature is 154 DEG C~156 DEG C, and secondary steam temperature is 41 DEG C~43
DEG C, cooling water inlet temperature is 19 DEG C~21 DEG C, and cooling water outlet temperature is 25 DEG C~27 DEG C.
Further, the preparation method of above-mentioned rose liquid distilled from honeysuckle flowers or lotus leaves is that fresh rose flower is obtained the rose through cryogenic vacuum distillation
Rare liquid distilled from honeysuckle flowers or lotus leaves.
Preferably, the cryogenic vacuum distillation is distilled under being under 50-70 DEG C and 0.05-0.1Mpa vacuum degree.
The present invention has the advantage that
1, it is provided by the invention with alpha-amylase inhibitory activity Hericium erinaceus compounding drinks in, sericin peptide taken have compared with
Strong alpha-amylase inhibitory activity；Water-soluble polysaccharide is to alpha-amylase also inhibitory activity with higher, Ke Yiyu in Hericium erinaceus
Alpha-amylase forms complexing agent and inactivates alpha-amylase；Flavone compound in Angelica Keiskei can be by inhibiting alpha-amylase
Activity delay the hydrolysis of starch.In addition, flavone compound can also inhibit internal by induced glucose transporter
Absorption to glucose.Therefore, the compounding drinks in the present invention have the alpha-amylase inhibitory activity of collaboration enhancing, because
This, it is edible which is suitable for the special populations such as diabetic and overweight people.
2, the Hericium erinaceus provided by the invention with alpha-amylase inhibitory activity compounds drinks, by rose liquid distilled from honeysuckle flowers or lotus leaves fragrance
Nutrition and function of the ingredient with the water-soluble functional component in Hericium erinaceus, Angelica Keiskei are organically blent, and product is made to have profit
Skin beauty, nourishing stomach and spleen, the multiple health-care effect for inhibiting alpha-amylase activity, improving the immunity of the human body, and with rose
The mouthfeel of fragrance and Hericium erinaceus, Angelica Keiskei sweetness, marketing and application prospect are extensive.
3, the silk gum peptide extract that the present invention adds in formula design, the amino acid containing there are many, such as serine, sweet ammonia
Acid, asparatate, alanine etc. have bioactivity, such as anti-oxidant, prevention and treatment diabetes function.The present invention answers sericin peptide taken
It uses in beverage, improves the trophic function value of beverage.
4, the ultrahigh-temperature instant sterilization technology that the present invention uses in process, increases the stability of beverage product, therefore,
Addition stabilizer is not needed in drink formula design.This can not only guarantee that the nutriment loss of beverage is small, but also into one
Step improves the edible safety of beverage.
The technical effect of design of the invention, specific steps and generation will be described further below, with fully
Solve the purpose of the present invention, feature and effect.
Specific embodiment
In a specific embodiment of the invention, unless otherwise specified, used raw material and instrument can be directly from markets
Purchase obtains.
The zymolyte of sericin peptide taken in the present invention can be obtained directly from Huzhou Xintiansi Biotechnology Co. Ltd.'s purchase.
In the present invention, " solid-liquid ratio " refers to mass volume ratio, and unit is " g/mL ".
In a specific embodiment of the invention, Hericium erinaceus extract is obtained by the method for hot water extraction combination alcohol precipitation, should
Hericium erinaceus extract includes water-soluble polysaccharide, proteins,vitamins,minerals, unsaturated fatty acid, amino acid etc..
In a specific embodiment of the invention, leaf extract will be to combine the method being concentrated by evaporation by hot water extraction tomorrow,
It is especially obtained in conjunction with the evaporation and concentration of film-rising evaporator, leaf extract will include cumarin, polysaccharide, protein and Huang tomorrow
Letones etc..
In a specific embodiment of the invention, silk gum peptide extract is the zymolyte by sericin peptide taken by obtaining after mwco membrane
The supernatant obtained especially obtains sericin peptide taken semifinished product in conjunction with the evaporation and concentration of film-rising evaporator, then leads to by being concentrated by evaporation
It crosses alcohol extracting and freeze-drying obtains the silk gum peptide extract.Wherein, the relative molecular weight of the sericin peptide taken of acquisition is less than 3000Da.
In a specific embodiment of the invention, rose liquid distilled from honeysuckle flowers or lotus leaves is prepared as follows: rose fresh flower is passed through low temperature
The method of vacuum distillation, i.e., the dry rose at 50-70 DEG C of temperature and 0.05-0.1Mpa vacuum degree, obtains distillate, distillation
The aqueous portion of liquid is rose liquid distilled from honeysuckle flowers or lotus leaves.The rose liquid distilled from honeysuckle flowers or lotus leaves includes citronellol, geraniol, nerol, borneol and eugenol etc..
Hericium erinaceus extract, leaf extract tomorrow, silk gum peptide extract measure the inhibiting rate of alpha-amylase in the present invention
The result is as follows:
Embodiment 1
A kind of Hericium erinaceus compounding drinks with alpha-amylase inhibitory activity, are made by the steps:
1, the Hericium erinaceus dried at 50 DEG C is crushed, it is mixed by 1:20 (g/mL) solid-liquid ratio crosses 60 meshes, hedgehog hydnum mushroom powder and water
It closes uniformly, 80 DEG C of hot water extraction 2h, then is centrifuged 15min under the conditions of 8000r/min, the supernatant after taking centrifugation is freeze-dried
Hedgehog hydnum mushroom powder.Hedgehog hydnum mushroom powder is uniformly mixed with 80% ethyl alcohol by 1:20 (g/mL) solid-liquid ratio, and 2h is stood at -20 DEG C, collects precipitating
Object, obtained by freeze drying Hericium erinaceus extract.
2, clean fresh angelica keiskei is shredded, is uniformly mixed with water by 1:8 solid-liquid ratio, 60 DEG C of hot water extraction 45min, so
Afterwards in film-rising evaporator (feeding temperature: 52.3 DEG C, drop temperature: 67.9 DEG C, raw vapor (steam) temperature: 107.8 DEG C, secondary steaming
Stripping temperature: 66.9 DEG C, cooling water inlet temperature: 15.3 DEG C, cooling water outlet temperature: 29.3 DEG C) it is concentrated, the sample after concentration
Product be spray-dried (inlet air temperature: 150 DEG C, leaving air temp: 70 DEG C, charging rate: 500mL/h) Angelica Keiskei powder.Tomorrow
75% ethyl alcohol of Ye Fenyu is uniformly mixed by solid-liquid ratio 1:5,1.5h is stood at 55 DEG C, then through being centrifuged 5min under the conditions of 5000r/min,
Take centrifugation after supernatant be freeze-dried tomorrow leaf extract.
3, by sericin peptide taken zymolyte, the film that molecular cut off is 3K is crossed, obtains supernatant, film-rising evaporator (charging temperature
Degree: 44 DEG C, drop temperature: 41.5 DEG C, raw vapor (steam) temperature: 155.5 DEG C, secondary steam temperature: 41.6 DEG C, cooling water inlet temperature
Degree: 20.4 DEG C, cooling water outlet temperature: 25.7 DEG C) after concentrating sample silk gum Gly-His-Lys.Silk gum Gly-His-Lys and 80% ethyl alcohol press 1:80
Solid-liquid ratio is uniformly mixed, and 2.5h is stood at -20 DEG C, obtains supernatant, freeze-dried to obtain the silk gum peptide extract.
4,0.004g Hericium erinaceus extract, 0.3g leaf extract tomorrow, 0.004g silk gum peptide extract, 6g xylose are weighed
Alcohol, 0.002g steviol glycoside, 0.3g citric acid and 5mL rose liquid distilled from honeysuckle flowers or lotus leaves are mixed, and 88.39mL drinking pure water is then added,
Make each component dissolution completely, is uniformly mixed, obtains mixing liquid one.
5, above-mentioned mixed liquor one is centrifuged under 2000r/min revolving speed 20min, collects supernatant, obtains mixed solution two.
6, above-mentioned mixed solution two is placed in homogeneous 5min under 300Mpa pressure, obtains mixed solution three.
7, above-mentioned mixed solution three is placed under 0.1Mpa vacuum degree the 15min that deaerates, obtains mixed solution four.
8, above-mentioned mixed solution four is placed in ultrahigh-temperature instant at 132 DEG C and sterilizes 5s, obtain mixed solution five.
9, mixing liquid five obtained above is placed at 82 DEG C carry out it is filling.
Embodiment 2
A kind of Hericium erinaceus compounding drinks with alpha-amylase inhibitory activity, are made by the steps:
1, the Hericium erinaceus dried at 50 DEG C is crushed, crosses 80 meshes, then hedgehog hydnum mushroom powder is mixed with water by 1:30 solid-liquid ratio
Uniformly, 85 DEG C of hot water extraction 1h, then it is centrifuged 15min under the conditions of 8000r/min, the supernatant after taking centrifugation is freeze-dried to obtain monkey
Head mushroom powder.Hedgehog hydnum mushroom powder is uniformly mixed with 80% ethyl alcohol by 1:30 solid-liquid ratio, stands 3 hours at -23 DEG C, collects sediment, warp
It is freeze-dried and Hericium erinaceus extract is made.
2, clean fresh angelica keiskei is shredded, is uniformly mixed with water by 1:6 solid-liquid ratio, 55 DEG C of hot water extraction 50min, so
Afterwards in film-rising evaporator (feeding temperature: 52.5 DEG C, drop temperature: 67.4 DEG C, raw vapor (steam) temperature: 107.4 DEG C, secondary steaming
Stripping temperature: 66.7 DEG C, cooling water inlet temperature: 15 DEG C, cooling water outlet temperature: 29 DEG C) be concentrated, the sample after concentration into
Row spray drying (inlet air temperature: 148 DEG C, leaving air temp: 69 DEG C, charging rate: 500mL/h) obtain Angelica Keiskei powder.Angelica Keiskei powder
It is uniformly mixed with 75% ethyl alcohol by solid-liquid ratio 1:8,1h is stood at 60 DEG C, then be centrifuged 5min under the conditions of 5000r/min, take centrifugation
Supernatant afterwards be freeze-dried tomorrow leaf extract.
3, by sericin peptide taken zymolyte, the film that molecular cut off is 3K is crossed, obtains supernatant, film-rising evaporator (charging temperature
Degree: 44.5 DEG C, drop temperature: 41.3 DEG C, raw vapor (steam) temperature: 155 DEG C, secondary steam temperature: 41 DEG C, cooling water inlet temperature:
20 DEG C, cooling water outlet temperature: 25.5 DEG C) after concentrating sample silk gum Gly-His-Lys.Silk gum Gly-His-Lys and 80% ethyl alcohol press 1:100 feed liquid
Than being uniformly mixed, 3h is stood at -18 DEG C, obtains supernatant, freeze-drying obtains the silk gum peptide extract.
4,0.009g Hericium erinaceus extract, 0.38g leaf extract tomorrow, 0.01g silk gum peptide extract, 4g xylose are weighed
Alcohol, 0.001g steviol glycoside, 0.1g citric acid and 1.5mL rose liquid distilled from honeysuckle flowers or lotus leaves are mixed, and it is pure that drinking for 94mL is then added
Water makes each component dissolution completely, is uniformly mixed, obtains mixing liquid one.
5, above-mentioned mixed liquor one is centrifuged under 3000r/min revolving speed 15min, collects supernatant, obtains mixed solution two.
6, above-mentioned mixed solution two is placed in homogeneous 10min under 250Mpa pressure, obtains mixed solution three.
7, above-mentioned mixed solution three is placed under 0.1Mpa vacuum degree the 10min that deaerates, obtains mixed solution four.
8, above-mentioned mixed solution four is placed in ultrahigh-temperature instant at 135 DEG C and sterilizes 3s, obtain mixed solution five.
9, mixing liquid five obtained above is placed at 83 DEG C carry out it is filling.
Embodiment 3
A kind of Hericium erinaceus compounding drinks with alpha-amylase inhibitory activity, are made by the steps:
1, the Hericium erinaceus dried at 50 DEG C is crushed, it is mixed by 1:15 (g/mL) solid-liquid ratio crosses 40 meshes, hedgehog hydnum mushroom powder and water
It closes uniformly, 75 DEG C of hot water extraction 3h, then is centrifuged 15min under the conditions of 8000r/min, the supernatant after taking centrifugation is freeze-dried
Hedgehog hydnum mushroom powder.Hedgehog hydnum mushroom powder is uniformly mixed with 80% ethyl alcohol by 1:10 (g/mL) solid-liquid ratio, and 1h is stood at -18 DEG C, collects precipitating
Object, obtained by freeze drying Hericium erinaceus extract.
2, clean fresh angelica keiskei is shredded, is uniformly mixed with water by 1:5 solid-liquid ratio, 50 DEG C of hot water extraction 60min, so
Afterwards in film-rising evaporator (feeding temperature: 51 DEG C, drop temperature: 67 DEG C, raw vapor (steam) temperature: 106 DEG C, secondary steam temperature:
66 DEG C, cooling water inlet temperature: 14 DEG C, cooling water outlet temperature: 29 DEG C) it is concentrated, the sample after concentration is done by spraying
Dry (inlet air temperature: 152 DEG C, leaving air temp: 71 DEG C, charging rate: 502mL/h) obtain Angelica Keiskei powder.Angelica Keiskei powder and 75% second
Alcohol is uniformly mixed by solid-liquid ratio 1:3,2h is stood at 50 DEG C, then be centrifuged 5min under the conditions of 5000r/min, the supernatant after taking centrifugation
Liquid be freeze-dried tomorrow leaf extract.
3, by sericin peptide taken zymolyte, the film that molecular cut off is 3K is crossed, obtains supernatant, film-rising evaporator (charging temperature
Degree: 43 DEG C, drop temperature: 40 DEG C, raw vapor (steam) temperature: 154 DEG C, secondary steam temperature: 41 DEG C, cooling water inlet temperature: 19 DEG C,
Cooling water outlet temperature: 25 DEG C) after concentrating sample silk gum Gly-His-Lys.Silk gum Gly-His-Lys are mixed with 80% ethyl alcohol by 1:60 solid-liquid ratio
It is even, 2h is stood at -22 DEG C, obtains supernatant, it is freeze-dried to obtain the silk gum peptide extract.
4,0.003g Hericium erinaceus extract, 0.05g leaf extract tomorrow, 0.003g silk gum peptide extract, 2g malt are weighed
Sugar alcohol, 0.1g malic acid and 1mL rose liquid distilled from honeysuckle flowers or lotus leaves are mixed, and 88mL drinking pure water is then added, has dissolved each component
Entirely, it is uniformly mixed, obtains mixing liquid one.
5, above-mentioned mixed liquor one is centrifuged under 4000r/min revolving speed 10min, collects supernatant, obtains mixed solution two.
6, above-mentioned mixed solution two is placed in homogeneous 10min under 200Mpa pressure, obtains mixed solution three.
7, above-mentioned mixed solution three is placed under 0.1Mpa vacuum degree the 13min that deaerates, obtains mixed solution four.
8, above-mentioned mixed solution four is placed in ultrahigh-temperature instant at 130 DEG C and sterilizes 8s, obtain mixed solution five.
9, mixing liquid five obtained above is placed at 80 DEG C carry out it is filling.
Embodiment 4
A kind of Hericium erinaceus compounding drinks with alpha-amylase inhibitory activity, are made by the steps:
1, the Hericium erinaceus dried at 50 DEG C is crushed, it is mixed by 1:20 (g/mL) solid-liquid ratio crosses 60 meshes, hedgehog hydnum mushroom powder and water
It closes uniformly, 80 DEG C of hot water extraction 2h, then is centrifuged 15min under the conditions of 8000r/min, the supernatant after taking centrifugation is freeze-dried
Hedgehog hydnum mushroom powder.Hedgehog hydnum mushroom powder is uniformly mixed with 80% ethyl alcohol by 1:25 (g/mL) solid-liquid ratio, and 2.5h is stood at -20 DEG C, and it is heavy to collect
Starch, obtained by freeze drying Hericium erinaceus extract.
2, clean fresh angelica keiskei is shredded, is uniformly mixed with water by 1:10 solid-liquid ratio, 70 DEG C of hot water extraction 30min,
Then in film-rising evaporator (feeding temperature: 53 DEG C, drop temperature: 69 DEG C, raw vapor (steam) temperature: 108 DEG C, secondary steam temperature
Degree: 68 DEG C, cooling water inlet temperature: 16 DEG C, cooling water outlet temperature: 31 DEG C) it is concentrated, the sample after concentration is sprayed
Dry (inlet air temperature: 149 DEG C, leaving air temp: 69 DEG C, charging rate: 498mL/h) obtain Angelica Keiskei powder.Angelica Keiskei powder and 75%
Ethyl alcohol is uniformly mixed by solid-liquid ratio 1:6,2h is stood at 55 DEG C, then be centrifuged 5min under the conditions of 5000r/min, upper after taking centrifugation
Clear liquid be freeze-dried tomorrow leaf extract.
3, by sericin peptide taken zymolyte, the film that molecular cut off is 3K is crossed, obtains supernatant, film-rising evaporator (charging temperature
Degree: 45 DEG C, drop temperature: 42 DEG C, raw vapor (steam) temperature: 156 DEG C, secondary steam temperature: 42 DEG C, cooling water inlet temperature: 21 DEG C,
Cooling water outlet temperature: 27 DEG C) after concentrating sample silk gum Gly-His-Lys.Silk gum Gly-His-Lys are mixed with 80% ethyl alcohol by 1:90 solid-liquid ratio
It is even, 2h is stood at -20 DEG C, obtains supernatant, it is freeze-dried to obtain the silk gum peptide extract.
4,0.01g Hericium erinaceus extract, 0.5g leaf extract tomorrow, 0.01g silk gum peptide extract, 8g sorbose are weighed
Alcohol, 0.003g Aspartame, 0.3g tartaric acid and 5mL rose liquid distilled from honeysuckle flowers or lotus leaves are mixed, and 96mL drinking pure water is then added, makes
Each component dissolution completely, is uniformly mixed, obtains mixing liquid one.
5, above-mentioned mixed liquor one is centrifuged under 3000r/min revolving speed 15min, collects supernatant, obtains mixed solution two.
6, above-mentioned mixed solution two is placed in homogeneous 10min under 250Mpa pressure, obtains mixed solution three.
7, above-mentioned mixed solution three is placed under 0.1Mpa vacuum degree the 13min that deaerates, obtains mixed solution four.
8, above-mentioned mixed solution four is placed in ultrahigh-temperature instant at 135 DEG C and sterilizes 3s, obtain mixed solution five.
9, mixing liquid five obtained above is placed at 85 DEG C carry out it is filling.
The preferred embodiment of the present invention has been described in detail above.It should be appreciated that the ordinary skill of this field is without wound
The property made labour, which according to the present invention can conceive, makes many modifications and variations.Therefore, all technician in the art
Pass through the available technology of logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Scheme, all should be within the scope of protection determined by the claims.
Claims (6)
1. a kind of Hericium erinaceus with alpha-amylase inhibitory activity compounds beverage, which is characterized in that preparation method includes following
Step:
1) leaf extract tomorrow, Hericium erinaceus extract, silk gum peptide extract, sugar alcohol, low calorie sweetener, tart flavour ingredient: are weighed
Agent, rose liquid distilled from honeysuckle flowers or lotus leaves and water are simultaneously uniformly mixed, and obtain mixed solution one；
2) it is centrifuged: the mixed solution one being centrifuged, supernatant is taken；
3) homogeneous: the supernatant is subjected to homogeneous, obtains mixed solution two；
4) it deaerates: the mixed solution two is de-gassed；
5) it sterilizes: two ultrahigh-temperature instant of mixed solution after degassing is sterilized；
6) filling: the mixed solution two after ultrahigh-temperature instant is sterilized carries out filling, there is alpha-amylase inhibitory activity described in acquisition
Hericium erinaceus compound beverage, wherein
The group of the parts by weight of the raw material of Hericium erinaceus compounding beverage be grouped as include: the Hericium erinaceus extract 0.003~
0.01 part, 0.05~0.5 part of leaf extract tomorrow, 0.003~0.01 part of the silk gum peptide extract, the sugar alcohol 2~8
Part, 0.000~0.003 part of the low calorie sweetener, 0.1~0.3 part of the acid, 1~5 part of the rose liquid distilled from honeysuckle flowers or lotus leaves, water
88~96 parts；
The silk gum peptide extract the preparation method comprises the following steps: by the zymolyte of the sericin peptide taken cross molecular cut off be 3K film, obtain
Take supernatant one；The supernatant one is evaporated concentration, obtains silk gum Gly-His-Lys；The silk gum Gly-His-Lys are pressed with 80% ethyl alcohol
1:60~1:100 solid-liquid ratio is uniformly mixed, and stands 2~3 hours at -18 DEG C~-22 DEG C, and centrifugation obtains supernatant two, will be described
Supernatant two carries out freeze-drying and obtains the silk gum peptide extract；
The relative molecular weight of the silk gum peptide extract is less than 3000Da；
The preparation method of the rose liquid distilled from honeysuckle flowers or lotus leaves is that fresh rose flower is obtained the rose liquid distilled from honeysuckle flowers or lotus leaves through cryogenic vacuum distillation.
2. the Hericium erinaceus with alpha-amylase inhibitory activity compounds beverage as described in claim 1, which is characterized in that the monkey
Head mushroom extract includes at least water-soluble polysaccharide, minerals and protein in Hericium erinaceus.
3. the Hericium erinaceus with alpha-amylase inhibitory activity compounds beverage as described in claim 1, which is characterized in that stated clearly
Day, leaf extract was including at least cumarin, polysaccharide, protein and the Flavonoid substances in Angelica Keiskei.
4. the Hericium erinaceus with alpha-amylase inhibitory activity compounds beverage as described in claim 1, which is characterized in that the rose
Rare liquid distilled from honeysuckle flowers or lotus leaves includes at least citronellol, geraniol, nerol, borneol and eugenol.
5. the Hericium erinaceus with alpha-amylase inhibitory activity compounds beverage as described in claim 1, which is characterized in that the monkey
Head mushroom extract the preparation method comprises the following steps: the Hericium erinaceus of drying crushed 40~80 meshes；By the crude hedgehog hydnum mushroom powder of acquisition with
Water is uniformly mixed by 1:15~1:30 solid-liquid ratio, is extracted 1~3 hour in 75 DEG C~85 DEG C hot water, and centrifugation obtains supernatant, cold
Dry, acquisition hedgehog hydnum mushroom powder is lyophilized；The hedgehog hydnum mushroom powder is uniformly mixed with 80% ethyl alcohol by 1:10~1:30 solid-liquid ratio, -18
DEG C~-23 DEG C at stand 1~3 hour, collect sediment；Sediment freeze-drying is obtained into the Hericium erinaceus extract.
6. the Hericium erinaceus with alpha-amylase inhibitory activity compounds beverage as described in claim 1, which is characterized in that stated clearly
Day leaf extract the preparation method comprises the following steps: clean fresh angelica keiskei clean, chopping；It is mixed with water by 1:5~1:10 solid-liquid ratio
It is even, it is extracted 0.5~1 hour in 50 DEG C~70 DEG C hot water, centrifugation obtains supernatant three；The supernatant three is evaporated dense
Contracting；Solution after concentration is spray-dried, Angelica Keiskei powder is obtained；The Angelica Keiskei powder and 75% ethyl alcohol are pressed into 1:3~1:8
Solid-liquid ratio is uniformly mixed, and stands 1~2 hour at 50 DEG C~60 DEG C, and centrifugation obtains supernatant four；The supernatant four is carried out
Freeze-drying obtains leaf extract tomorrow.
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