CN105794616A - Seed nutrient solution cultivation method - Google Patents
Seed nutrient solution cultivation method Download PDFInfo
- Publication number
- CN105794616A CN105794616A CN201610208073.9A CN201610208073A CN105794616A CN 105794616 A CN105794616 A CN 105794616A CN 201610208073 A CN201610208073 A CN 201610208073A CN 105794616 A CN105794616 A CN 105794616A
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- Prior art keywords
- seed
- germination
- breeding method
- solution
- nutritional
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
- C05G5/23—Solutions
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Soil Sciences (AREA)
- Pretreatment Of Seeds And Plants (AREA)
- Hydroponics (AREA)
Abstract
Provided is a seed nutrient solution cultivation method.The method comprises the steps of pretreatment, wherein seeds are dried under the sun for 1 d to 3 d, and the seeds are subjected to disinfection; low-temperature sleep treatment, wherein the seeds subjected to the pretreatment are put in the environment of 5 DEG C for 5 d to 7 d and then put in the environment of 10 DEG C for 5 d to 10 d; seed germination, wherein the seeds are put into deionized water for soaking and then taken out for seed germination, constant seed germination temperature is set, and water is sprayed on the seeds at a set time each day; strong seedling transplanting, wherein after the seeds are terminated and the seedlings grow to 2.5 cm to 3.5 cm, the seedlings are transplanted, and the culture medium is quartz sand; nutrient solution cultivation, wherein when the seedlings grow to have two true leaves, the seedlings are transplanted to a nutrient solution from the quartz sand.The method is economical, environmentally friendly, low in pollution and good in tissue culture effect.
Description
Technical field
The present invention relates to field of plant growing technology, be specifically related to a kind of seed nutritional liquid breeding method.
Background technology
Herba Verbenae majority is grown on open country, and in China East China, also there is distribution on the ground such as south China, its have removing heat from blood, dissipating blood stasis, heat clearing away, removing toxic substances,
The effect such as antipruritic, therefore becomes a kind of important draft, and the yield of Herba Verbenae little at present, and cultivation period is long before existing, and
Quality is the highest, and excellent seed is the primary condition that can obtain high yield, and the nutrient solution culture of optimization is bred as commonly using for Herba Verbenae cultivating seeds
Mode, in prior art, the method for seed culture is limited to, and does not has a kind of economic and environment-friendly, the culture fluid of of low pollution, and growth cycle
Long.
Summary of the invention
It is an object of the invention to overcome the deficiencies in the prior art, it is provided that a kind of economic and environment-friendly, and pollute little, the seed that group training is effective
Nutritional solution breeding method.
The invention provides a kind of seed nutritional liquid breeding method, comprise the steps:
(1) pre-treatment step: by seed dry in the sun 1-3d under sunlight condition, then seed is carried out disinfection；
(2) processing stage of low temperature sleep: seed after pre-treatment in step (1) is placed in 5-7d in the environment of 5 DEG C, is being placed in
5-10d in the environment of 10 DEG C；
(3) in the germination stage: step (2) gained seed is placed in deionized water immersion, then takes out, treat germination, arrange
Constant germination temperature, sprays water to seed in the set time every day；
(4) transplant the stage in strong sprout: after germination to seedling grow to 2.5～3.5cm time, transplanted seedlings, culture matrix now is stone
Sand；
(5) the Solution culture method stage: when growth of seedling to two panels true leaf, move on to normal growth nutritional solution from quartz sand；
Wherein, seed nutritional liquid is grouped into by the one-tenth with following mass percent:
Preferably, described in step (1), sterilization method is: the seed going out decontamination is soaked in 40% sulfuric acid solution 15～25min,
Go sulfuric acid, add after deionized water cleans seed 4-5 time repeatedly and remove deionized water, then seed is soaked in the ethanol solution of 45%
20～40min, remove ethanol, add deionized water and clean seed 3～5 times.
Preferably, described in step (3), soak time is 6-12 hour.
Preferably, described in step (3), germination temperature is 30～33 DEG C.
Preferably, nutritional solution described in step (5) uses 1/5 concentration in the cultivation incipient stage, uses 1/3 concentration after one week.
Preferably, the method moving on to nutritional solution described in step (5) is: joined by nutritional solution in lighttight container, and container cover is many
Orifice plate, wraps up seedling with sponge strip more than root, inserts in container cover porous plate down by the shoot root system having wrapped up sponge, culture fluid
Liquid level is completely covered shoot root, and then container is placed under culturing rack sufficient illumination cultivation
Preferably, seed is Folium Salicis Babylonicae Herba Verbenae seed.
The seed nutritional liquid breeding method of the present invention, it is possible to achieve:
1) use through germination, low temperature sleep process, transplant strong sprout and Solution culture method seedling each and every one stage several, finally cultivate seedling.
Having method simple to operation, the seedling time is relatively much shorter, specification consistency advantages of higher.
2) nutritional solution used by and conventional nutraceutical liquid phase ratio, greatly reduce the consumption of compound, decreases the pollution that nutritional solution produces,
Nutritional solution is easily recycled, and the amino acids material and the Chinese medicine class material that add in nutritional solution create synergism, compensate for chemical classes chemical combination
Thing consumption is not enough, reduces pollution simultaneously, improves seed viability, shortens germinating time.
3) extract solution of bamboo leaves of appropriate amount is added, it is possible to promoting germination, the addition of organic selenium improves the nutritional labeling of nutritional solution,
It also is able to promote germination.
Detailed description of the invention
Being embodied as the following detailed description of the present invention, it is necessary to it is pointed out here that, below implement to be only intended to the present invention furtherly
Bright, it is impossible to be interpreted as limiting the scope of the invention, this art skilled person make the present invention according to the invention described above content
Some nonessential improvement and adjustment, still fall within protection scope of the present invention.
The invention provides a kind of seed nutritional liquid breeding method, comprise the steps:
(1) pre-treatment step: by seed dry in the sun 1-3d under sunlight condition, then seed is carried out disinfection, described sterilization method
For: the seed going out decontamination is soaked 15～25min in 40% sulfuric acid solution, goes sulfuric acid, add deionized water and repeatedly clean seed
Remove deionized water after 4-5 time, then seed is soaked in the ethanol solution of 45% 20～40min, remove ethanol, add deionization
Water cleans seed 3～5 times；
(2) processing stage of low temperature sleep: seed after pre-treatment in step (1) is placed in 5-7d in the environment of 5 DEG C, is being placed in
5-10d in the environment of 10 DEG C；
(3) in the germination stage: step (2) gained seed is placed in deionized water immersion, soak time is 6-12 hour, then
Taking out, treat germination, arrange constant germination temperature, germination temperature is 30～33 DEG C, and seed is entered by every day in the set time
Row water spray；
(4) transplant the stage in strong sprout: after germination to seedling grow to 2.5～3.5cm time, transplanted seedlings, culture matrix now is stone
Sand；
(5) the Solution culture method stage: when growth of seedling to two panels true leaf, move on to normal growth nutritional solution from quartz sand；
Wherein, seed nutritional liquid is grouped into by the one-tenth with following mass percent:
Wherein nutritional solution uses 1/5 concentration in the cultivation incipient stage, uses 1/3 concentration after one week.The method moving on to nutritional solution is: by nutritional solution
Joining in lighttight container, container cover is porous plate, is wrapped up with sponge strip by seedling, will wrap up the shoot root of sponge more than root
System inserts in container cover porous plate down, and culture fluid liquid level is completely covered shoot root, and then container is placed under culturing rack sufficient illumination cultivation.
Wherein, seed can be Folium Salicis Babylonicae Herba Verbenae seed.
Comparative example 1
Change nutrient composition and carry out Experimental comparison's (without extract solution of bamboo leaves):
Seed nutritional liquid is grouped into by the one-tenth with following mass percent:
Cultivating through 2～3 weeks nutritional solution stages, Folium Salicis Babylonicae Herba Verbenae Seedling more than 92% reaches 5 leaves.
Comparative example 2
Change nutrient composition and carry out Experimental comparison's (without aminoacid ingredient):
Seed nutritional liquid is grouped into by the one-tenth with following mass percent:
Cultivating through 2～3 weeks nutritional solution stages, Folium Salicis Babylonicae Herba Verbenae Seedling more than 89% reaches 5 leaves.
Comparative example 3
Change nutrient composition and carry out Experimental comparison's (without Chinese medicine class extracting solution):
Seed nutritional liquid is grouped into by the one-tenth with following mass percent:
Cultivating through 2～3 weeks nutritional solution stages, Folium Salicis Babylonicae Herba Verbenae Seedling more than 88% reaches 5 leaves.
Without vitamin B5 or organic selenium in the most each embodiment, Folium Salicis Babylonicae Herba Verbenae Seedling respectively more than 90% reaches 5 leaves, and 92
More than % reaches 5 leaves.
Although for illustrative purposes, it has been described that the illustrative embodiments of the present invention, it will be understood by those of skill in the art that not
In the case of departing from the scope and spirit of the invention disclosed in claims, various amendment, interpolation can be carried out in form and details
With the change of replacement etc., and all these change all should belong to the protection domain of claims of the present invention, and claimed
Each department of product and method in each step, can combine with form in any combination.Therefore, public to institute in the present invention
The description of the embodiment opened is not intended to limit the scope of the present invention, but is used for describing the present invention.Correspondingly, the scope of the present invention is not
Limited by embodiment of above, but be defined by claim or its equivalent.
Claims (7)
1. a seed nutritional liquid breeding method, it is characterised in that: comprise the steps:
(1) pre-treatment step: by seed dry in the sun 1-3d under sunlight condition, then seed is carried out disinfection；
(2) processing stage of low temperature sleep: seed after pre-treatment in step (1) is placed in 5-7d in the environment of 5 DEG C, 5-10d in the environment of being placed in 10 DEG C；
(3) in the germination stage: step (2) gained seed is placed in deionized water immersion, then takes out, treat germination, constant germination temperature is set, in the set time, seed is sprayed water every day；
(4) transplant the stage in strong sprout: after germination to seedling grow to 2.5～3.5cm time, transplanted seedlings, culture matrix now is quartz sand；
(5) the Solution culture method stage: when growth of seedling to two panels true leaf, move on to normal growth nutritional solution from quartz sand；
Wherein, seed nutritional liquid is grouped into by the one-tenth with following mass percent:
。
2. breeding method as claimed in claim 1, it is characterized in that: described in step (1), sterilization method is: the seed going out decontamination is soaked 15～25min in 40% sulfuric acid solution, go sulfuric acid, add after deionized water cleans seed 4-5 time repeatedly and remove deionized water, seed is soaked in the ethanol solution of 45% 20～40min again, remove ethanol, add deionized water and clean seed 3～5 times.
3. breeding method as claimed in claim 1, it is characterised in that: described in step (3), soak time is 6-12 hour.
4. breeding method as claimed in claim 1, it is characterised in that: described in step (3), germination temperature is 30～33 DEG C.
5. breeding method as claimed in claim 1, it is characterised in that: nutritional solution described in step (5) uses 1/5 concentration in the cultivation incipient stage, uses 1/3 concentration after one week.
6. breeding method as claimed in claim 1, it is characterized in that: the method moving on to nutritional solution described in step (5) is: joined by nutritional solution in lighttight container, container cover is porous plate, seedling is wrapped up with sponge strip more than root, the shoot root system having wrapped up sponge is inserted in container cover porous plate down, culture fluid liquid level is completely covered shoot root, and then container is placed under culturing rack sufficient illumination cultivation.
7. the breeding method as described in any one of claim 1-6, it is characterised in that: seed is Folium Salicis Babylonicae Herba Verbenae seed.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610208073.9A CN105794616A (en) | 2016-04-06 | 2016-04-06 | Seed nutrient solution cultivation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610208073.9A CN105794616A (en) | 2016-04-06 | 2016-04-06 | Seed nutrient solution cultivation method |
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| CN105794616A true CN105794616A (en) | 2016-07-27 |
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Family Applications (1)
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| CN201610208073.9A Pending CN105794616A (en) | 2016-04-06 | 2016-04-06 | Seed nutrient solution cultivation method |
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Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102584397A (en) * | 2012-02-17 | 2012-07-18 | 南京工业大学 | Water culturing nutrient solution as well as preparation method and application thereof |
| CN104472233A (en) * | 2014-12-15 | 2015-04-01 | 成都苗夫现代苗木科技有限公司 | Cutting propagation method of verbena bonariensis |
| CN104541918A (en) * | 2015-01-28 | 2015-04-29 | 南农大(常熟)新农村发展研究院有限公司 | Cultivation method capable of preventing lettuce oversummer bolting |
| CN104719159A (en) * | 2015-03-13 | 2015-06-24 | 广东省农业科学院作物研究所 | Nutrient solution and method for applying nutrient solution to stem tissue-cultured seedling of dendrobium officinale |
| CN105075454A (en) * | 2015-09-07 | 2015-11-25 | 江苏农林职业技术学院 | Indoor sprouting experiment method for preventing seeds from molding |
| CN105144911A (en) * | 2015-09-28 | 2015-12-16 | 江苏农林职业技术学院 | Method for cultivating grass-like plant |
| CN105237156A (en) * | 2015-09-09 | 2016-01-13 | 安徽稻稼云社生物科技有限公司 | High-purity liquid fertilizer having seedling strengthening function and preparation method thereof |
| JP2016034243A (en) * | 2014-08-01 | 2016-03-17 | 株式会社インタートレード | Hydroponic method and hydroponic apparatus of panax ginseng |
-
2016
- 2016-04-06 CN CN201610208073.9A patent/CN105794616A/en active Pending
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102584397A (en) * | 2012-02-17 | 2012-07-18 | 南京工业大学 | Water culturing nutrient solution as well as preparation method and application thereof |
| JP2016034243A (en) * | 2014-08-01 | 2016-03-17 | 株式会社インタートレード | Hydroponic method and hydroponic apparatus of panax ginseng |
| CN104472233A (en) * | 2014-12-15 | 2015-04-01 | 成都苗夫现代苗木科技有限公司 | Cutting propagation method of verbena bonariensis |
| CN104541918A (en) * | 2015-01-28 | 2015-04-29 | 南农大(常熟)新农村发展研究院有限公司 | Cultivation method capable of preventing lettuce oversummer bolting |
| CN104719159A (en) * | 2015-03-13 | 2015-06-24 | 广东省农业科学院作物研究所 | Nutrient solution and method for applying nutrient solution to stem tissue-cultured seedling of dendrobium officinale |
| CN105075454A (en) * | 2015-09-07 | 2015-11-25 | 江苏农林职业技术学院 | Indoor sprouting experiment method for preventing seeds from molding |
| CN105237156A (en) * | 2015-09-09 | 2016-01-13 | 安徽稻稼云社生物科技有限公司 | High-purity liquid fertilizer having seedling strengthening function and preparation method thereof |
| CN105144911A (en) * | 2015-09-28 | 2015-12-16 | 江苏农林职业技术学院 | Method for cultivating grass-like plant |
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Application publication date: 20160727 |
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