CN105779519A - Preparation method of silkworm chrysalis amino acid - Google Patents
Preparation method of silkworm chrysalis amino acid Download PDFInfo
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Abstract
The invention discloses a preparation method of silkworm chrysalis amino acid. The preparation method comprises the following steps of: (1) smashing silkworm chrysalis into 5-35 meshes, then mixing with water in a mass ratio of (1-10):(40-80), decocting for 30-180 minutes at the temperature of 80-100 DEG C, cooling to 10-30 DEG C, and regulating pH to 4-11, so that decocted solution is obtained; (2) adding enzyme and an enzyme live keeping agent into the decocted solution, hydrolyzing for 1-5 hours in water bath at the temperature of 45-75 DEG C, and then inactivating the enzyme for 5-15 minutes in water bath at the temperature of 85-100 DEG C, so that enzymatic hydrolysate is obtained; and (3) sequentially carrying out filtration by virtue of a plate-and-frame filter, filtration by virtue of a polyvinylidene difluoride ultrafiltration membrane, reverse osmosis membrane concentration, drying and sterilization on the enzymatic hydrolysate, so that the silkworm chrysalis amino acid is obtained. The preparation method disclosed by the invention for silkworm chrysalis amino acid takes silkworm chrysalis as a raw material, decocting, enzymolysis, filtration and concentration are carried out, and then freeze-drying is carried out, so that the silkworm chrysalis amino acid is obtained. Compared with the prior art, the preparation method disclosed by the invention has the advantages that extraction ratio of an extraction process is high, no acid or base is used in the whole production process, desalination or decoloration is carried out, and clean production of the silkworm chrysalis amino acid is realized.
Description
Technical field
The present invention relates to nutraceutical field, be specifically related to the preparation method of a kind of abundant amino acids from silkworm pupa.
Background technology
Pupa bombycis is the pupa of silkworm section insecticide bombyx mori Bomby x mori L, nutritious, and medical value is high,
Its medicinal record is just had in the ancient books and records such as Compendium of Material Medica.Modern medicine proves, Pupa bombycis has fall
The effects such as low cholesterolemia, treatment coronary insufficiency.The silk yield of China occupies first of the world,
While manufacturing raw silk, the most large side-product is Pupa bombycis.Report according to relevant information, often manufacture
1 ton of raw silk is achieved with 1 ton of Pupa bombycis, therefore Pupa bombycis quantity is many.Pupa bombycis also has more rich money in Yunnan Province
Source, only Luliang silk textile industry company the most just have more than 1000 ton of Pupa bombycis to produce.Owing to lacking research, Pupa bombycis
Substantially being used to do animal feed or be eaten, resource utilization and added value are extremely low.
In the modern times, Pupa bombycis by medical evidence have promotion liver cell regeneration and lipid metabolism, the liver protecting,
Slow down aging, raising intelligence, protection vision, enhancing immune function, blood sugar lowering, treatment coronary artery supply
Insufficiency of blood and the pharmacological action such as anticancer.Use the egg that biological engineering and zymolysis technique extract from Pupa bombycis
White matter hydrolyzate contains rich in protein and several amino acids, not only to leukemia, marrow failure,
The disease such as hypertension, tumor has treatment or the effect of auxiliary treatment, also can be developed into all types of Pupa bombycises multiple
Close amino acid nutrient health promoting product, often take and not only can supplement aminoacid raising physical function, also have
Blood fat reducing, enhancing immunity, improve cardiovascular function and prevention effect such as cardiovascular disease.And Pupa bombycis egg
The functions such as the blood pressure lowering of plain boiled water hydrolysis products, antioxidation, resisting fatigue more become separation and prepare biological activity
Peptide and develop functional food, the quality raw materials of medicine, have the highest nutrition, medicinal Development volue and
Market prospect.
The invention provides the preparation method of a kind of abundant amino acids from silkworm pupa, extraction ratio is high.
Summary of the invention
For above-mentioned deficiency present in prior art, the technical problem to be solved is to provide one
Plant preparation method of abundant amino acids from silkworm pupa and preparation method thereof.
The present invention seeks to be achieved through the following technical solutions:
The preparation method of a kind of abundant amino acids from silkworm pupa, comprises the following steps:
(1) dried silkworm chrysalis meal is broken to 5-35 mesh, then is (1-10) in mass ratio with water: (40-80) mixes
Close, decoct 30-180 minute at 80-100 DEG C, be cooled to 10-30 DEG C, and regulate pH to 4-11,
Obtain decoction liquor；
(2) enzyme and enzyme live keeping agent addition decoction liquor will hydrolyze under 45-75 DEG C of water-bath 1-5 hour, so
After under 85-100 DEG C of water-bath enzyme denaturing live 5-15 minute, obtain enzymolysis solution；
(3) by the filtration of enzymolysis solution successively flame filter press, polyvinylidene fluoride ultrafiltration membrane filtration, reverse osmosis
Membrance concentration, dry, sterilizing, to obtain final product.
In described step (1), regulation pH value can use NaOH solution, HCl solution etc..
Preferably, the 1-10% that addition is Pupa bombycis quality of enzyme in described step (2), enzyme live keeping agent
Addition is the 0.2-2% of Pupa bombycis quality.
Preferably, the enzyme in described step (2) is in papain, trypsin, bromelain
The mixture of one or more.
It is highly preferred that the enzyme in described step (2) is by papain, trypsin, bromelain
Mixing, described papain, trypsin, the mass ratio of bromelain are (1-3): (1-3):
(1-3)。
Preferably, the enzyme live keeping agent in described step (2) is shell six sugar, hydroxyl isomaltulose, chitotriose
In one or more mixture.
It is highly preferred that the enzyme live keeping agent in described step (2) is by shell six sugar, hydroxyl isomaltulose, shell three
Sugar mixes, and described shell six sugar, hydroxyl isomaltulose, the mass ratio of chitotriose are (1-3): (1-3):
(1-3)。
Preferably, the flame filter press in described step (3) filters, and filter cloth is 100-300 mesh.
Preferably, the aperture of the polyvinylidene fluoride ultrafiltration film in described step (3) is 0.1-0.5 μm.
Preferably, the aperture that the reverse osmosis membrane in described step (3) concentrates is 0.0001-0.001 μm.
Preferably, being dried in described step (3) is dried for atomizer, press spray is dried,
One or more in lyophilization, vacuum drying.
The invention discloses the preparation method of a kind of abundant amino acids from silkworm pupa, with Pupa bombycis as raw material, through decoction, enzyme
Solve, filter, concentrate after lyophilizing prepare.Compared with prior art, the extraction process extraction ratio of the present invention is high,
Whole production process is without soda acid, without desalination and decoloration process, it is achieved that the cleaning of abundant amino acids from silkworm pupa produces.
Detailed description of the invention
Below in conjunction with embodiment, the present invention is described further, the following stated, only to the present invention
Preferred embodiment, not does the restriction of other forms, any technology being familiar with this specialty to the present invention
Personnel are changed to the Equivalent embodiments changed on an equal basis possibly also with the technology contents of the disclosure above.Every
Without departing from the present invention program content, any letter following example done according to the technical spirit of the present invention
Single amendment or equivalent variations, all fall within protection scope of the present invention.
Each raw material introduction in embodiment:
Shell six sugar, No. CAS: 41708-95-6.
Hydroxyl isomaltulose, No. CAS: 64519-82-0.
Chitotriose, No. CAS: 41708-93-4.
Papain, No. CAS: 9001-73-4, Zhengzhou Kang Yuan chemical products company limited provides, enzyme
Live 200U/mg.
Trypsin, No. CAS: 9002-07-7, Zhengzhou good brother chemical products company limited provides, enzyme
Live 200U/mg.
Bromelain, No. CAS: 9001-00-7, Sheng Xie bio tech ltd, Shandong provides, enzyme
Live 200U/mg.
Pupa bombycis, uses the mulberry silkworm chrysalis that Chuzhou City great Feng plant provides.
Embodiment 1
The concrete preparation method of abundant amino acids from silkworm pupa, comprises the following steps:
(1) dried silkworm chrysalis meal being broken to 10 mesh, the 0.6kg Pupa bombycis after pulverizing is mixed with the stirring of 6kg distilled water
Close uniformly, decoct 90 minutes at 95 DEG C, be cooled to 20 DEG C, and with the sodium hydroxide solution of 2mol/L
Regulation pH to 7.8, obtains decoction liquor；
(2) 60 DEG C of water-baths after 18g enzyme and 6g enzyme live keeping agent addition decoction liquor being uniformly mixed
Lower hydrolysis 2 hours, then under 95 DEG C of water-baths, enzyme denaturing is lived 10 minutes, obtains enzymolysis solution；
(3) using flame filter press to filter enzymolysis solution, filter cloth is 180 mesh；Then gather through 0.3 μm
Vinylidene fluoride ultrafiltration membrance filter；Via hole diameter is that 0.0001 μm reverse osmosis membrane concentrates, through-20 DEG C of lyophilizing again
Cheng Fen, after through ultra-vioket radiation, irradiation time is 5-15 second sterilizing.Obtain abundant amino acids from silkworm pupa.
Enzyme in described step (2) by papain, trypsin, bromelain is in mass ratio
1:1:1 is uniformly mixed and obtains.
Enzyme live keeping agent in described step (2) is by shell six sugar, hydroxyl isomaltulose, chitotriose in mass ratio
It is uniformly mixed for 1:1:1 and obtains.
Embodiment 2
Substantially the same manner as Example 1, differ only in: described enzyme is by papain, Trypsin
Enzyme is uniformly mixed for 1:1 in mass ratio and obtains.Can be prepared by the abundant amino acids from silkworm pupa of embodiment 2.
Embodiment 3
Substantially the same manner as Example 1, differ only in: described enzyme is by papain, Fructus Ananadis comosi egg
White enzyme is uniformly mixed for 1:1 in mass ratio and obtains.Can be prepared by the abundant amino acids from silkworm pupa of embodiment 3.
Embodiment 4
Substantially the same manner as Example 1, differ only in: described enzyme is by trypsin, bromelain
Enzyme is uniformly mixed for 1:1 in mass ratio and obtains.Can be prepared by the abundant amino acids from silkworm pupa of embodiment 4.
Embodiment 5
Substantially the same manner as Example 1, differ only in: described enzyme live keeping agent wheat sugared by shell six, different
Bud ketose alcohol is uniformly mixed for 1:1 in mass ratio and obtains.Can be prepared by the Pupa bombycis amino of embodiment 5
Acid.
Embodiment 6
Substantially the same manner as Example 1, differ only in: described enzyme live keeping agent is by shell six sugar, shell three
Sugar is uniformly mixed for 1:1 in mass ratio and obtains.Can be prepared by the abundant amino acids from silkworm pupa of embodiment 6.
Embodiment 7
Substantially the same manner as Example 1, differ only in: described enzyme live keeping agent by hydroxyl isomaltulose,
Chitotriose is uniformly mixed for 1:1 in mass ratio and obtains.Can be prepared by the abundant amino acids from silkworm pupa of embodiment 7.
Test case 1
In the abundant amino acids from silkworm pupa preparing embodiment 1-7, amino acid content is tested, at Hitachi 835-50
Detect on type automatic amino acid analyzer.Concrete outcome is shown in Table 1.
Table 1: abundant amino acids from silkworm pupa content results list position: %
| Total amino acids content | Isoleucine | Methionine | Leucine | Lysine | |
| Embodiment 1 | 38.6 | 2.4 | 1.2 | 3.1 | 2.7 |
| Embodiment 2 | 36.5 | 2.2 | 1.0 | 3.0 | 2.4 |
| Embodiment 3 | 37.2 | 2.1 | 0.9 | 2.9 | 2.6 |
| Embodiment 4 | 36.1 | 2.2 | 0.9 | 3.0 | 2.3 |
| Embodiment 5 | 37.3 | 2.3 | 1.0 | 2.9 | 2.4 |
| Embodiment 6 | 35.4 | 2.1 | 1.1 | 2.8 | 2.5 |
| Embodiment 7 | 3.5.8 | 2.1 | 0.9 | 2.8 | 2.2 |
Comparing embodiment 1 and embodiment 2-4, embodiment 1 (papain, trypsin, Fructus Ananadis comosi egg
White enzyme) abundant amino acids from silkworm pupa content is apparently higher than embodiment 2-4 (papain, trypsin, Fructus Ananadis comosi
In protease, any two kinds compound).Comparing embodiment 1 and embodiment 5-7, embodiment 1 (shell six sugar,
Hydroxyl isomaltulose, chitotriose) (shell six is sugared, different apparently higher than embodiment 2-4 for abundant amino acids from silkworm pupa content
In maltulose alcohol, chitotriose, any two kinds compound).
Test case 2
Abundant amino acids from silkworm pupa embodiment 1-7 prepared, is placed in 25 DEG C, preservation under relative humidity 85% environment
Half a year, " GB/T 4789.2-2010 microbiological test of food hygiene total plate count mensuration " is used to enter
Row total plate count is tested.Concrete test result is shown in Table 2.
Table 2: total plate count test table cfu/g
Inventor is found surprisingly that, after adding enzyme and enzyme live keeping agent, total plate count is decreased obviously, product
Anti-microbial property significantly improve, the shelf-life is significantly extended.Comparing embodiment 1 and embodiment 2-4,
Embodiment 1 (papain, trypsin, bromelain) anti-microbial property is apparently higher than embodiment
2-4 (in papain, trypsin, bromelain, any two kinds compound).Comparing embodiment 1
With embodiment 5-7, embodiment 1 (shell six sugar, hydroxyl isomaltulose, chitotriose) anti-microbial property is the highest
In embodiment 2-4 (in shell six sugar, hydroxyl isomaltulose, chitotriose, any two kinds compound).
Claims (6)
1. the preparation method of an abundant amino acids from silkworm pupa, it is characterised in that comprise the following steps:
(1) dried silkworm chrysalis meal is broken to 5-35 mesh, then is (1-10) in mass ratio with water: (40-80) mixes
Close, decoct 30-180 minute at 80-100 DEG C, be cooled to 10-30 DEG C, and regulate pH to 4-11,
Obtain decoction liquor；
(2) enzyme and enzyme live keeping agent addition decoction liquor will hydrolyze under 45-75 DEG C of water-bath 1-5 hour, so
After under 85-100 DEG C of water-bath enzyme denaturing live 5-15 minute, obtain enzymolysis solution；
(3) by the filtration of enzymolysis solution successively flame filter press, polyvinylidene fluoride ultrafiltration membrane filtration, reverse osmosis
Membrance concentration, dry, sterilizing, to obtain final product.
2. the preparation method of abundant amino acids from silkworm pupa as claimed in claim 1, it is characterised in that: described step
(2) in, the addition of enzyme is the 1-10% of Pupa bombycis quality, and the addition of enzyme live keeping agent is Pupa bombycis quality
0.2-2%.
3. the preparation method of abundant amino acids from silkworm pupa as claimed in claim 1 or 2, it is characterised in that: described
Enzyme in step (2) is the mixing of one or more in papain, trypsin, bromelain
Thing.
4. the preparation method of abundant amino acids from silkworm pupa as claimed in claim 3, it is characterised in that: described step
(2) enzyme in is mixed by papain, trypsin, bromelain, described Papain
Enzyme, trypsin, the mass ratio of bromelain are (1-3): (1-3): (1-3).
5. the preparation method of abundant amino acids from silkworm pupa as claimed in claim 1 or 2, it is characterised in that: described
Enzyme live keeping agent in step (2) is one or more mixed in shell six sugar, hydroxyl isomaltulose, chitotriose
Compound.
6. the preparation method of abundant amino acids from silkworm pupa as claimed in claim 5, it is characterised in that: described step
(2) the enzyme live keeping agent in is mixed by shell six sugar, hydroxyl isomaltulose, chitotriose, described shell six sugar,
Hydroxyl isomaltulose, the mass ratio of chitotriose are (1-3): (1-3): (1-3).
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN106636276A (en) * | 2016-12-22 | 2017-05-10 | 山东明鑫集团有限公司 | Method for preparing silkworm pupa isolated protein powder modified by zirconium sulfate |
| CN106755236A (en) * | 2016-12-22 | 2017-05-31 | 山东明鑫集团有限公司 | A kind of preparation method of decolouring silkworm separated protein powder |
| CN106723070A (en) * | 2016-11-24 | 2017-05-31 | 江苏爱西施科技服务咨询股份有限公司 | A kind of method for preparing compound amino acid as raw material with silkworm chrysalis |
| CN106755235A (en) * | 2016-12-22 | 2017-05-31 | 山东明鑫集团有限公司 | A kind of preparation method of pupa albumen Gly-His-Lys |
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