CN105779519B - The preparation method of abundant amino acids from silkworm pupa - Google Patents
The preparation method of abundant amino acids from silkworm pupa Download PDFInfo
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- 241000255789 Bombyx mori Species 0.000 title claims abstract description 52
- 150000001413 amino acids Chemical class 0.000 title claims abstract description 28
- 241000382353 Pupa Species 0.000 title claims abstract description 26
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 108090000790 Enzymes Proteins 0.000 claims abstract description 36
- 102000004190 Enzymes Human genes 0.000 claims abstract description 36
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 14
- 239000007788 liquid Substances 0.000 claims abstract description 8
- 239000002033 PVDF binder Substances 0.000 claims abstract description 5
- 239000012528 membrane Substances 0.000 claims abstract description 5
- 229920002981 polyvinylidene fluoride Polymers 0.000 claims abstract description 5
- 238000001223 reverse osmosis Methods 0.000 claims abstract description 5
- 238000000108 ultra-filtration Methods 0.000 claims abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 5
- 230000009849 deactivation Effects 0.000 claims abstract description 4
- 229940088598 enzyme Drugs 0.000 claims description 33
- 239000004365 Protease Substances 0.000 claims description 26
- SERLAGPUMNYUCK-DCUALPFSSA-N 1-O-alpha-D-glucopyranosyl-D-mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO[C@H]1O[C@H](CO)[C@@H](O)[C@H](O)[C@H]1O SERLAGPUMNYUCK-DCUALPFSSA-N 0.000 claims description 13
- 108010004032 Bromelains Proteins 0.000 claims description 13
- 108090000526 Papain Proteins 0.000 claims description 13
- 235000019835 bromelain Nutrition 0.000 claims description 13
- RQFQJYYMBWVMQG-IXDPLRRUSA-N chitotriose Chemical compound O[C@@H]1[C@@H](N)[C@H](O)O[C@H](CO)[C@H]1O[C@H]1[C@H](N)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O2)N)[C@@H](CO)O1 RQFQJYYMBWVMQG-IXDPLRRUSA-N 0.000 claims description 13
- 229940055729 papain Drugs 0.000 claims description 13
- 235000019834 papain Nutrition 0.000 claims description 13
- 235000010439 isomalt Nutrition 0.000 claims description 12
- 239000000905 isomalt Substances 0.000 claims description 12
- HPIGCVXMBGOWTF-UHFFFAOYSA-N isomaltol Natural products CC(=O)C=1OC=CC=1O HPIGCVXMBGOWTF-UHFFFAOYSA-N 0.000 claims description 12
- -1 trypsase Proteins 0.000 claims description 7
- 238000001035 drying Methods 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 claims 1
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims 1
- NBGXQZRRLOGAJF-UHFFFAOYSA-N Maltulose Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)(CO)OCC1O NBGXQZRRLOGAJF-UHFFFAOYSA-N 0.000 claims 1
- JCQLYHFGKNRPGE-HFZVAGMNSA-N maltulose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JCQLYHFGKNRPGE-HFZVAGMNSA-N 0.000 claims 1
- 238000001914 filtration Methods 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 239000002253 acid Substances 0.000 abstract description 5
- 238000000034 method Methods 0.000 abstract description 4
- 239000002994 raw material Substances 0.000 abstract description 4
- 238000011084 recovery Methods 0.000 abstract description 3
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 abstract description 2
- 238000010612 desalination reaction Methods 0.000 abstract description 2
- 230000007071 enzymatic hydrolysis Effects 0.000 abstract description 2
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 abstract description 2
- 238000000605 extraction Methods 0.000 abstract description 2
- 230000008569 process Effects 0.000 abstract description 2
- 229940024606 amino acid Drugs 0.000 description 23
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 6
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 6
- 238000012360 testing method Methods 0.000 description 5
- 238000013329 compounding Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 229910021529 ammonia Inorganic materials 0.000 description 3
- 230000000845 anti-microbial effect Effects 0.000 description 3
- 239000003814 drug Substances 0.000 description 2
- 239000004744 fabric Substances 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 230000036039 immunity Effects 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000002417 nutraceutical Substances 0.000 description 2
- 235000021436 nutraceutical agent Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000001151 other effect Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- KJBQDYDTQCIAFO-ZLELNMGESA-N (2s)-2-amino-4-methylpentanoic acid;(2s)-2,6-diaminohexanoic acid Chemical compound CC(C)C[C@H](N)C(O)=O.NCCCC[C@H](N)C(O)=O KJBQDYDTQCIAFO-ZLELNMGESA-N 0.000 description 1
- NREKYJNUNHNXAF-DHYYHALDSA-N (2s,3s)-2-amino-3-methylpentanoic acid;(2s)-2-amino-4-methylsulfanylbutanoic acid Chemical compound CC[C@H](C)[C@H](N)C(O)=O.CSCC[C@H](N)C(O)=O NREKYJNUNHNXAF-DHYYHALDSA-N 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 206010052895 Coronary artery insufficiency Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 108010009736 Protein Hydrolysates Proteins 0.000 description 1
- 238000009098 adjuvant therapy Methods 0.000 description 1
- 230000003712 anti-aging effect Effects 0.000 description 1
- 230000001093 anti-cancer Effects 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 210000001367 artery Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 230000036772 blood pressure Effects 0.000 description 1
- 230000036770 blood supply Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 230000009084 cardiovascular function Effects 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 230000004438 eyesight Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 239000000413 hydrolysate Substances 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000002218 hypoglycaemic effect Effects 0.000 description 1
- 208000032839 leukemia Diseases 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 210000005229 liver cell Anatomy 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 238000012009 microbiological test Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
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- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- General Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention discloses a kind of preparation method of abundant amino acids from silkworm pupa, the following steps are included: silkworm chrysalis is crushed to 5-35 mesh by (1), be in mass ratio with water again (1-10): (40-80) is mixed, it is decocted 30-180 minutes at 80-100 DEG C, it is cooled to 10-30 DEG C, and pH to 4-11 is adjusted, obtain decoction liquor；(2) enzyme and enzyme live keeping agent are added in decoction liquor and are hydrolyzed under 45-75 DEG C of water-bath 1-5 hours, then enzyme deactivation is 5-15 minutes living under 85-100 DEG C of water-bath, obtains enzymolysis liquid；(3) by enzymolysis liquid successively flame filter press filtering, the filtering of polyvinylidene fluoride ultrafiltration film, reverse osmosis membrane concentration, it is dry, sterilize to get.The invention discloses a kind of preparation methods of abundant amino acids from silkworm pupa, using silkworm chrysalis as raw material, are lyophilized and are made after decoction, enzymatic hydrolysis, filtering, concentration.Compared with prior art, extraction process recovery rate of the invention is high, and whole production process is not necessarily to soda acid, no desalination and decoloration process, realizes the clean manufacturing of abundant amino acids from silkworm pupa.
Description
Technical field
The present invention relates to nutraceutical fields, and in particular to a kind of preparation method of abundant amino acids from silkworm pupa.
Background technique
Silkworm chrysalis is the pupa of silkworm section insect bombyx mori Bomby x mori L, full of nutrition, and medical value is high, early in " this
Careless detailed outline " etc. just have its medicinal in ancient books and records record.Modern medicine proves that silkworm chrysalis has reduction cholesterolemia, treatment coronal dynamic
Arteries and veins blood supply insufficiency and other effects.The silk yield in China occupies first of the world, and while manufacturing raw silk, most large byproduct is
Silkworm chrysalis.It is reported according to relevant information, 1 ton of raw silk of every manufacture is achieved with 1 ton of silkworm chrysalis, therefore silkworm chrysalis quantity is more.Silkworm chrysalis is in Yunnan Province
Also there is more rich resource, only silk textile industry company in Luliang just there are more than 1000 tons of silkworm chrysalises to generate every year.Due to lacking research, silkworm chrysalis
Substantially it is used to do animal feed or be eaten, resource utilization and added value are extremely low.
In the modern times, silkworm chrysalis had by medical evidence promote liver cell regeneration and fat metabolism, protection liver, anti-aging,
Improve intelligence, protection eyesight, enhancing immunity function, hypoglycemic, the treatment pharmacological actions such as coronary insufficiency and anticancer.Fortune
The protein hydrolysate protein rich in and a variety of ammonia extracted from silkworm chrysalis with bioengineering and zymolysis technique
The diseases such as leukaemia, marrow failure, hypertension, tumour are not only had the effect for the treatment of or adjuvant treatment, also can be developed by base acid
All types of silkworm compound amino acid nutraceutical products, amino acid can not only be supplemented by, which often taking, improves physical function, also has
There are reducing blood lipid, strengthen immunity, improve cardiovascular function and prevention cardiovascular disease and other effects.And pupa albumen hydrolysate
The functions such as blood pressure lowering, anti-oxidant, antifatigue more become separation and prepare biologically active peptide and develop functional food, drug
Quality raw materials have very high nutrition, medicinal Development volue and market prospects.
The present invention provides a kind of preparation method of abundant amino acids from silkworm pupa, recovery rate is high.
Summary of the invention
Aiming at the above shortcomings existing in the prior art, technical problem to be solved by the invention is to provide a kind of silkworm chrysalis ammonia
The preparation method and preparation method thereof of base acid.
Object of the present invention is to what is be achieved through the following technical solutions:
A kind of preparation method of abundant amino acids from silkworm pupa, comprising the following steps:
(1) silkworm chrysalis is crushed to 5-35 mesh, then is in mass ratio (1-10) with water: (40-80) is mixed, at 80-100 DEG C
It decocts 30-180 minutes, is cooled to 10-30 DEG C, and adjust pH to 4-11, obtains decoction liquor；
(2) enzyme and enzyme live keeping agent are added in decoction liquor and are hydrolyzed 1-5 hours under 45-75 DEG C of water-bath, then in 85-100
Enzyme deactivation is 5-15 minutes living under DEG C water-bath, obtains enzymolysis liquid；
(3) by enzymolysis liquid, successively flame filter press is filtered, polyvinylidene fluoride ultrafiltration film filters, reverse osmosis membrane concentration, does
It is dry, sterilizing to get.
PH value is adjusted in the step (1) can be using NaOH solution, HCl solution etc..
Preferably, the additional amount of enzyme is the 1-10% of silkworm chrysalis quality in the step (2), and the additional amount of enzyme live keeping agent is silkworm
The 0.2-2% of pupal mass.
Preferably, the enzyme in the step (2) be papain, trypsase, it is one or more in bromelain
Mixture.
It is highly preferred that the enzyme in the step (2) is mixed by papain, trypsase, bromelain, institute
State papain, trypsase, bromelain mass ratio be (1-3): (1-3): (1-3).
Preferably, the enzyme live keeping agent in the step (2) is six sugar of shell, isomalt, one kind or more in chitotriose
The mixture of kind.
It is highly preferred that the enzyme live keeping agent in the step (2) is mixed by six sugar of shell, isomalt, chitotriose,
Six sugar of shell, isomalt, chitotriose mass ratio be (1-3): (1-3): (1-3).
Preferably, the flame filter press filtering in the step (3), filter cloth are 100-300 mesh.
Preferably, the aperture of the polyvinylidene fluoride ultrafiltration film in the step (3) is 0.1-0.5 μm.
Preferably, the aperture of the reverse osmosis membrane concentration in the step (3) is 0.0001-0.001 μm.
Preferably, the drying in the step (3) is that atomizer is dry, press spray is dry, is freeze-dried, is true
One of sky drying is a variety of.
The invention discloses a kind of preparation methods of abundant amino acids from silkworm pupa, using silkworm chrysalis as raw material, through decoction, enzymatic hydrolysis, filtering, dense
It is lyophilized and is made after contracting.Compared with prior art, extraction process recovery rate of the invention is high, whole production process is not necessarily to soda acid, no desalination
With decoloration process, the clean manufacturing of abundant amino acids from silkworm pupa is realized.
Specific embodiment
The present invention will be further explained with reference to the examples below, as described below, is only to preferable implementation of the invention
Example, not limits the present invention, any person skilled in the art is possibly also with the disclosure above
Technology contents be changed to the equivalent embodiment changed on an equal basis.Without departing from the concept of the present invention, according to the present invention
Technical spirit any simple modification or equivalent variations that following embodiment is made, fall within the scope of protection of the present invention.
Each raw material introduction in embodiment:
Six sugar of shell, No. CAS: 41708-95-6.
Isomalt, No. CAS: 64519-82-0.
Chitotriose, No. CAS: 41708-93-4.
Papain, No. CAS: 9001-73-4, Zhengzhou Kang Yuan chemical products Co., Ltd provides, enzyme activity 200U/mg.
Trypsase, No. CAS: 9002-07-7, the good fraternal chemical products Co., Ltd in Zhengzhou provides, enzyme activity 200U/mg.
Bromelain, No. CAS: 9001-00-7, Shandong Sheng Xie Biotechnology Co., Ltd provides, enzyme activity 200U/mg.
Silkworm chrysalis, the mulberry silkworm chrysalis provided using Chuzhou City great Feng farm.
Embodiment 1
The specific preparation method of abundant amino acids from silkworm pupa, comprising the following steps:
(1) silkworm chrysalis is crushed to 10 mesh, smashed 0.6kg silkworm chrysalis is uniformly mixed with 6kg distilled water, at 95 DEG C
It is lower to decoct 90 minutes, 20 DEG C are cooled to, and adjust pH to 7.8 with the sodium hydroxide solution of 2mol/L, obtain decoction liquor；
(2) hydrolysis 2 is small under 60 DEG C of water-baths after being uniformly mixed in 18g enzyme and 6g enzyme live keeping agent addition decoction liquor
When, then enzyme deactivation is 10 minutes living under 95 DEG C of water-baths, obtains enzymolysis liquid；
(3) enzymolysis liquid is filtered using flame filter press, filter cloth is 180 mesh；Then through 0.3 μm of polyvinylidene fluoride ultrafiltration
Film filtering；Via hole diameter is 0.0001 μm of reverse osmosis membrane concentration, is lyophilized into powder through -20 DEG C again, most afterwards through ultraviolet irradiation, irradiation time
It sterilized for 5-15 seconds.Obtain abundant amino acids from silkworm pupa.
Enzyme in the step (2) is 1:1:1 stirring by papain, trypsase, bromelain in mass ratio
It is uniformly mixed and obtains.
Enzyme live keeping agent in the step (2) is stirred in mass ratio for 1:1:1 by six sugar of shell, isomalt, chitotriose
It mixes to be uniformly mixed and obtain.
Embodiment 2
Substantially the same manner as Example 1, difference is only in that: the enzyme by papain, trypsase in mass ratio
It is uniformly mixed to obtain for 1:1.It can be prepared by the abundant amino acids from silkworm pupa of embodiment 2.
Embodiment 3
Substantially the same manner as Example 1, difference is only in that: the enzyme is by papain, bromelain by quality
Than being uniformly mixed to obtain for 1:1.It can be prepared by the abundant amino acids from silkworm pupa of embodiment 3.
Embodiment 4
Substantially the same manner as Example 1, difference is only in that: the enzyme by trypsase, bromelain in mass ratio
It is uniformly mixed to obtain for 1:1.It can be prepared by the abundant amino acids from silkworm pupa of embodiment 4.
Embodiment 5
Substantially the same manner as Example 1, difference is only in that: the enzyme live keeping agent is pressed by six sugar of shell, isomalt
Mass ratio is that 1:1 is uniformly mixed to obtain.It can be prepared by the abundant amino acids from silkworm pupa of embodiment 5.
Embodiment 6
Substantially the same manner as Example 1, difference is only in that: the enzyme live keeping agent by the sugar of shell six, chitotriose in mass ratio
It is uniformly mixed to obtain for 1:1.It can be prepared by the abundant amino acids from silkworm pupa of embodiment 6.
Embodiment 7
Substantially the same manner as Example 1, difference is only in that: the enzyme live keeping agent is pressed by isomalt, chitotriose
Mass ratio is that 1:1 is uniformly mixed to obtain.It can be prepared by the abundant amino acids from silkworm pupa of embodiment 7.
Test case 1
Amino acid content in the abundant amino acids from silkworm pupa of embodiment 1-7 preparation is tested, in Hitachi 835-50 type amino acid
It is detected on automatic analyzer.Concrete outcome is shown in Table 1.
Table 1: abundant amino acids from silkworm pupa content results list position: %
| Total amino acid content | Isoleucine | Methionine | Leucine | Lysine | |
| Embodiment 1 | 38.6 | 2.4 | 1.2 | 3.1 | 2.7 |
| Embodiment 2 | 36.5 | 2.2 | 1.0 | 3.0 | 2.4 |
| Embodiment 3 | 37.2 | 2.1 | 0.9 | 2.9 | 2.6 |
| Embodiment 4 | 36.1 | 2.2 | 0.9 | 3.0 | 2.3 |
| Embodiment 5 | 37.3 | 2.3 | 1.0 | 2.9 | 2.4 |
| Embodiment 6 | 35.4 | 2.1 | 1.1 | 2.8 | 2.5 |
| Embodiment 7 | 3.5.8 | 2.1 | 0.9 | 2.8 | 2.2 |
Comparing embodiment 1 and embodiment 2-4, embodiment 1 (papain, trypsase, bromelain) silkworm chrysalis ammonia
Base acid content is apparently higher than embodiment 2-4 (papain, trypsase, any two kinds of compoundings in bromelain).Compare
Embodiment 1 and embodiment 5-7, embodiment 1 (six sugar of shell, isomalt, chitotriose) abundant amino acids from silkworm pupa content are apparently higher than
Embodiment 2-4 (six sugar of shell, isomalt, any two kinds of compoundings in chitotriose).
Test case 2
Abundant amino acids from silkworm pupa prepared by embodiment 1-7 is placed in 25 DEG C, and preservation half a year under 85% environment of relative humidity uses
" measurement of GB/T 4789.2-2010 microbiological test of food hygiene total plate count " carries out total plate count test.Specific test knot
Fruit is shown in Table 2.
Table 2: total plate count tests table cfu/g
Inventor is found surprisingly that after addition enzyme and enzyme live keeping agent, total plate count is decreased obviously, the anti-microbial property of product
It significantly improves, the shelf-life is substantially extended.Comparing embodiment 1 and embodiment 2-4, embodiment 1 (papain, tryptose
Enzyme, bromelain) to be apparently higher than embodiment 2-4 (papain, trypsase, any in bromelain for anti-microbial property
Two kinds of compoundings).Comparing embodiment 1 and embodiment 5-7, embodiment 1 (six sugar of shell, isomalt, chitotriose) anti-microbial property
It is apparently higher than embodiment 2-4 (six sugar of shell, isomalt, any two kinds of compoundings in chitotriose).
Claims (2)
1. a kind of preparation method of abundant amino acids from silkworm pupa, which comprises the following steps:
(1) silkworm chrysalis is crushed to 5-35 mesh, then is in mass ratio (1-10) with water: (40-80) is mixed, and is decocted at 80-100 DEG C
30-180 minutes, it is cooled to 10-30 DEG C, and adjusts pH to 4-11, obtains decoction liquor；
(2) enzyme and enzyme live keeping agent are added in decoction liquor and are hydrolyzed 1-5 hours under 45-75 DEG C of water-bath, then in 85-100 DEG C of water
It is 5-15 minutes living to bathe lower enzyme deactivation, obtains enzymolysis liquid；
(3) by enzymolysis liquid, successively flame filter press is filtered, polyvinylidene fluoride ultrafiltration film filters, reverse osmosis membrane concentration, drying, goes out
Bacterium to get；
Enzyme live keeping agent in the step (2) is mixed by six sugar of shell, isomalt, chitotriose, and the shell six is sugared, different
Maltulose alcohol, chitotriose mass ratio be (1-3): (1-3): (1-3)；
Enzyme in the step (2) is mixed by papain, trypsase, bromelain, the papain,
Trypsase, bromelain mass ratio be (1-3): (1-3): (1-3).
2. the preparation method of abundant amino acids from silkworm pupa as described in claim 1, it is characterised in that: the addition of enzyme in the step (2)
Amount is the 1-10% of silkworm chrysalis quality, and the additional amount of enzyme live keeping agent is the 0.2-2% of silkworm chrysalis quality.
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| CN106723070A (en) * | 2016-11-24 | 2017-05-31 | 江苏爱西施科技服务咨询股份有限公司 | A kind of method for preparing compound amino acid as raw material with silkworm chrysalis |
| CN106755236A (en) * | 2016-12-22 | 2017-05-31 | 山东明鑫集团有限公司 | A kind of preparation method of decolouring silkworm separated protein powder |
| CN106636276A (en) * | 2016-12-22 | 2017-05-10 | 山东明鑫集团有限公司 | Method for preparing silkworm pupa isolated protein powder modified by zirconium sulfate |
| CN106755235A (en) * | 2016-12-22 | 2017-05-31 | 山东明鑫集团有限公司 | A kind of preparation method of pupa albumen Gly-His-Lys |
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| CN105077261A (en) * | 2015-09-24 | 2015-11-25 | 上海韬鸿化工科技有限公司 | Composite fruit and vegetable ferment and preparing method thereof |
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| CN101008026A (en) * | 2006-12-29 | 2007-08-01 | 周维海 | Method for preparing silkworm chrysalis polypeptide using complex enzyme for hydrolyzing silkworm chrysalis |
| CN101530232A (en) * | 2009-04-20 | 2009-09-16 | 广东环西保健科技股份有限公司 | Method for extracting compound amino acid from silkworm pupae by enzymatic method |
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| CN101748170A (en) * | 2010-01-22 | 2010-06-23 | 西北大学 | Method for preparing 1-deoxy-D-xylulose with chemical method-enzymatic method |
| CN102604747A (en) * | 2012-03-12 | 2012-07-25 | 南宁创新科技医药技术有限公司 | Technique for producing alpha-linoleic acid, silkworm pupa protein and silkworm pupa peptides with silkworm pupas |
| CN103540636A (en) * | 2013-09-29 | 2014-01-29 | 浙江省农业科学院 | Method for preparing blood fat reducing peptide by use of silkworm chrysalis |
| CN105077261A (en) * | 2015-09-24 | 2015-11-25 | 上海韬鸿化工科技有限公司 | Composite fruit and vegetable ferment and preparing method thereof |
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