CN114984098B - Hawthorn chicken gizzard-skin chewable tablet and preparation method thereof - Google Patents
Hawthorn chicken gizzard-skin chewable tablet and preparation method thereof Download PDFInfo
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- CN114984098B CN114984098B CN202210865396.0A CN202210865396A CN114984098B CN 114984098 B CN114984098 B CN 114984098B CN 202210865396 A CN202210865396 A CN 202210865396A CN 114984098 B CN114984098 B CN 114984098B
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- gizzard
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Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/73—Rosaceae (Rose family), e.g. strawberry, chokeberry, blackberry, pear or firethorn
- A61K36/734—Crataegus (hawthorn)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
- A61K35/57—Birds; Materials from birds, e.g. eggs, feathers, egg white, egg yolk or endothelium corneum gigeriae galli
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/0012—Galenical forms characterised by the site of application
- A61K9/0053—Mouth and digestive tract, i.e. intraoral and peroral administration
- A61K9/0056—Mouth soluble or dispersible forms; Suckable, eatable, chewable coherent forms; Forms rapidly disintegrating in the mouth; Lozenges; Lollipops; Bite capsules; Baked products; Baits or other oral forms for animals
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/20—Pills, tablets, discs, rods
- A61K9/2004—Excipients; Inactive ingredients
- A61K9/2022—Organic macromolecular compounds
- A61K9/205—Polysaccharides, e.g. alginate, gums; Cyclodextrin
- A61K9/2054—Cellulose; Cellulose derivatives, e.g. hydroxypropyl methylcellulose
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/0005—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts
- A61L2/0082—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor for pharmaceuticals, biologicals or living parts using chemical substances
- A61L2/0088—Liquid substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P1/00—Drugs for disorders of the alimentary tract or the digestive system
- A61P1/14—Prodigestives, e.g. acids, enzymes, appetite stimulants, antidyspeptics, tonics, antiflatulents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2101/00—Chemical composition of materials used in disinfecting, sterilising or deodorising
- A61L2101/02—Inorganic materials
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2202/00—Aspects relating to methods or apparatus for disinfecting or sterilising materials or objects
- A61L2202/20—Targets to be treated
- A61L2202/21—Pharmaceuticals, e.g. medicaments, artificial body parts
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Pharmacology & Pharmacy (AREA)
- Epidemiology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Nutrition Science (AREA)
- Botany (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Medical Informatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Mycology (AREA)
- Alternative & Traditional Medicine (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to the technical field of traditional Chinese medicine chewable tablets and discloses a haw chicken's gizzard-membrane chewable tablet and a preparation method thereof, wherein the chewable tablet comprises 20-30 parts of haw, 40-60 parts of chicken's gizzard-membrane extract, 10-25 parts of sweet orange powder, 30-50 parts of sodium carboxymethyl cellulose, 2-6 parts of microcrystalline cellulose, 5-15 parts of sodium alginate and 1-2 parts of magnesium stearate, and under the relatively mild extraction condition, effective components such as protein, polysaccharide, amino acid and the like in the chicken's gizzard-membrane are deeply extracted by using an ultrasonic auxiliary enzyme method, so that the damage to nutritional components in the chicken's gizzard-membrane by a high temperature processing mode similar to the processing modes such as stir-fried chicken's gizzard-membrane and sand-fried chicken's gizzard-membrane is avoided, the added sweet orange powder can change the taste of the chewable tablet, so that the taste of the chewable tablet is endowed with a certain sweet taste, and the taste of most people is more compounded, and the audience of the chewable tablet is widened.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicine chewable tablets, in particular to a hawthorn chicken's gizzard-skin chewable tablet and a preparation method thereof.
Background
Dyspepsia is a common functional gastrointestinal disease caused by gastric dyskinesia, is accompanied by symptoms of digestive function discomfort such as pain, distention, nausea, inappetence and the like, is a common clinical syndrome, and is mainly used as a medicament for treating dyspepsia at present, but western medicines generally have certain side effects, and the use of the Chinese medicines is relatively small in side effects, and the treatment effect is not inferior to western medicines, so that the dyspepsia is gradually accepted by patients, but the current prescription preparation for treating dyspepsia is many, but is mostly formed by medicines and is not treated with auxiliary treatment for a long time, so that a food therapy product for safe auxiliary treatment is developed, and has great significance and value.
The hawthorn contains various substances such as flavonoid, internal lipid, tartaric acid, vitamins and the like, has the effects of promoting digestion and resolving stagnation, dispersing qi and blood and the like, has wide application in the aspect of treating dyspepsia, contains various beneficial components such as gastrin, keratin, amino acid, pepsin and the like, has the effects of strengthening stomach and promoting digestion, resolving symptom and eliminating dampness and stopping seminal emission, combines the hawthorn with the chicken's gizzard-membrane, can generate excellent digestion promoting effect by mutual synergy, but has relatively more chemical components, can not extract various effective components of the chicken's gizzard-membrane, can easily cause loss of nutritional components, and has the advantages of improving the utilization rate of the effective components, such as the keratin, the amino acid, the pepsin and the like, of the chicken's gizzard-membrane by slicing, freeze-drying, jet milling and solvent extraction processes of the chicken's gizzard-membrane, overcomes the defect that the chicken's gizzard-membrane is difficult to extract a plurality of effective components, therefore, a special extraction mode is needed to be used for extracting the keratin, the amino acid, the pepsin and the like in the chicken's gizzard-membrane, the chicken's gizzard-membrane can be deeply extracted into medicinal tablets, and the medicinal raw materials are deeper than the medicinal tablet is obtained.
Disclosure of Invention
The invention aims to provide a hawthorn chicken's gizzard-membrane chewable tablet and a preparation method thereof, which solve the problems that nutrient substances are easy to lose and active ingredients are not completely extracted when chicken's gizzard-membrane is processed.
The aim of the invention can be achieved by the following technical scheme:
A chewable tablet comprising fructus crataegi and endothelium corneum Gigeriae Galli comprises fructus crataegi 20-30 parts, endothelium corneum Gigeriae Galli extract 40-60 parts, fructus Citri sinensis powder 10-25 parts, sodium carboxymethylcellulose 30-50 parts, sodium alginate 5-15 parts, and magnesium stearate 1-2 parts.
Further, the preparation method of the chicken's gizzard-membrane extract is as follows:
Step S1: crushing chicken's gizzard-membrane, adding into deionized water 10-30 times of the weight of the chicken's gizzard-membrane, transferring into an ultrasonic instrument, continuing to add complex enzyme for enzymolysis, heating to 90 ℃ after the enzymolysis is finished, preserving heat for 10-30min, inactivating the enzyme, filtering after cooling the solution, separating filtrate and filter residues, concentrating the filtrate, and drying to obtain chicken's gizzard-membrane enzyme extract powder;
Step S2: adding the filter residue obtained in the step S1 into 4mL of 1-2% hydrochloric acid solution, leaching at 40-50deg.C for 1-3h, filtering after extraction, concentrating the filtrate, and drying to obtain endothelium corneum Gigeriae Galli acid extract powder;
Step S3: mixing the powders of step S1 and step S2, and drying at 50-60deg.C until the water content is 0.5-2% to obtain endothelium corneum Gigeriae Galli extract.
According to the technical scheme, the ultrasonic-assisted enzymatic extraction mode is used for extracting the effective components such as proteins, amino acids and polysaccharides in the chicken's gizzard-membrane, the effective components in the chicken's gizzard-membrane can be fully extracted at a relatively low extraction temperature, the nutritional components in the effective components can not be destroyed, the pepsin and other components in the chicken's gizzard-membrane can be further extracted by the acid extraction mode, and the acid extraction mode can effectively improve the activity of the pepsin, so that the pepsin has better decomposition capacity on proteins and the like in the stomach, and good digestion promoting effect is achieved.
Further, the complex enzyme in the step S1 is cellulase and neutral protease in a weight ratio of 1:2.
Further, the amount of the complex enzyme in the step S1 is 1-2%.
Further, the temperature is set to be 50-60 ℃ and the frequency is 300-400W in the enzymolysis in the step S1, and the time is 2-4 hours.
Further, the specific method of filtering in the step S1 and the step S2 is that the prepared solution is filtered through a polyvinyl alcohol-silver-loaded titanium dioxide micro-filtration membrane.
Further, the preparation method of the polyvinyl alcohol-silver-loaded titanium dioxide micro-filtration membrane comprises the following steps:
(1) Carrying out surface modification on the nano titanium dioxide by using KH-550 to obtain aminated titanium dioxide;
(2) Dispersing the aminated titanium dioxide in an ethanol solvent, adding a silver nitrate solution, uniformly mixing, reacting for 1-3 hours at 10-30 ℃, adding sodium borohydride, continuously reacting for 1-2 hours, centrifuging, washing and drying to obtain silver-loaded titanium dioxide;
(3) Adding silver-carrying titanium dioxide into polyethylene glycol-1000 deionized water solution, uniformly dispersing by ultrasonic, adding polyvinyl alcohol aqueous solution, uniformly stirring, defoaming for 4-10h, uniformly coating the casting solution on a glass plate by using a scraper, casting to form a film, standing at room temperature, and removing the film to obtain the polyvinyl alcohol-silver-carrying titanium dioxide composite ultrafiltration membrane.
In the step (2), the weight ratio of the amination titanium dioxide to the silver nitrate to the sodium borohydride in the silver nitrate solution is 10:0.5-4:5-15.
In the step (3), silver-carrying titanium dioxide, polyethylene glycol in the polyethylene glycol-1000 aqueous solution and polyvinyl alcohol in the polyvinyl alcohol aqueous solution are in a weight ratio of 0.1-1:0.2-0.8:10.
According to the technical scheme, the nano titanium dioxide has good photocatalysis antibacterial performance, silver is further loaded, the antibacterial performance of the nano titanium dioxide can be effectively enhanced, the nano titanium dioxide is compounded with biodegradable polyvinyl alcohol, the formed polyvinyl alcohol-silver loaded titanium dioxide composite ultrafiltration membrane has excellent antibacterial performance, bacteria in the chicken's gizzard-membrane extract can be removed in the process of filtering the chicken's gizzard-membrane extract, the chicken's gizzard-membrane extract is in a sterile state, and convenience is brought to further preparing the chicken's gizzard-membrane chewable tablet.
Further, the preparation method of the haw and chicken's gizzard-skin chewable tablet is as follows:
step SS1: drying fructus crataegi, fructus Citri sinensis powder, microcrystalline cellulose, and sodium alginate, grinding, and sieving with 80-100 mesh sieve;
Step SS2: dissolving sodium carboxymethyl cellulose in deionized water to prepare suspension with the concentration of 8-20%;
step SS3: mixing endothelium corneum Gigeriae Galli extract, fructus crataegi, fructus Citri sinensis powder, and sodium alginate in equal amount, adding into suspension in step SS2, stirring while adding magnesium stearate, mixing, sieving with 16-20 mesh sieve, granulating, drying, tabletting with tabletting machine, and drying with vacuum drying method to obtain fructus crataegi and endothelium corneum Gigeriae Galli chewable tablet.
Further, the step SS3 is dried to a water content of 1-3%.
Through the technical scheme, the microcrystalline cellulose has good binding force and compressibility, can form a synergistic effect with the adhesive sodium carboxymethyl cellulose, improves the compressibility of the hawthorn chicken gizzard-membrane particles, and the sweet orange powder is natural fruit powder prepared from sweet oranges, contains rich natural sugar powder and vitamin C, has natural orange flavor, can be used as a corrigent on one hand, can form sweet and sour delicious taste by being matched with the sour taste of hawthorns on the other hand, and can further enhance the health care effect of the chewable tablet.
The invention has the beneficial effects that: by using an ultrasonic-assisted enzymatic extraction mode, under relatively mild extraction conditions, effective components such as protein, polysaccharide and amino acid in the chicken's gizzard-membrane are extracted deeply, so that the content of the protein in the chicken's gizzard-membrane extract is up to 6.2%, the content of the polysaccharide is up to 0.51%, the content of the amino acid is up to 64.8u/g, the damage of the high-temperature processing modes similar to stir-frying the chicken's gizzard-membrane, sand-frying the chicken's gizzard-membrane and the like to nutrient components in the chicken's gizzard-membrane is avoided, and through testing, the protease activity in the chicken's gizzard-membrane extract is up to 120.4u/g, and meanwhile, after the microcrystalline cellulose and the adhesive carboxymethyl cellulose sodium form a synergistic effect, a good enhancement effect is formed on the compressibility of the chewable tablet, so that granules can still meet the process requirement of molding under lower moisture content, the taste of the chewable tablet is improved by adding the sweet orange powder, the taste of the chewable tablet is endowed with a certain sweet taste, the taste of most people is compounded, the taste of the chewable tablet is widened, the digestion effect test shows that the prepared haw tablet has higher pepsin activity and good digestion effect.
Of course, it is not necessary for any one product to practice the invention to achieve all of the advantages set forth above at the same time.
Detailed Description
The technical solutions of the present invention will be clearly and completely described in connection with the embodiments, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
Preparation of a polyvinyl alcohol-silver-loaded titanium dioxide ultrafiltration membrane:
(1) Adding 0.5g of nano titanium dioxide into 60mL of toluene solvent, uniformly dispersing by ultrasonic, continuously adding 2mL of KH-550, reacting for 12 hours at 80 ℃, centrifuging, washing and drying the product to obtain aminated titanium dioxide;
(2) Dispersing 1g of aminated titanium dioxide in 50mL of ethanol solvent, adding silver nitrate solution containing 0.05g of silver nitrate, uniformly mixing, reacting for 1h at 10 ℃, adding 0.5g of sodium borohydride, continuously reacting for 1h, centrifuging, washing and drying to obtain silver-loaded titanium dioxide;
(3) Adding 0.01g of silver-carrying titanium dioxide into 0.02g of polyethylene glycol-1000 aqueous solution containing polyethylene glycol-1000, uniformly dispersing by ultrasonic, adding 1g of polyvinyl alcohol aqueous solution containing polyvinyl alcohol, uniformly stirring, defoaming for 4 hours, uniformly coating the casting solution on a glass plate by using a scraper, casting to form a film, standing at room temperature, and removing the film to obtain the polyvinyl alcohol-silver-carrying titanium dioxide composite ultrafiltration membrane.
Example 2
Preparation of a polyvinyl alcohol-silver-loaded titanium dioxide ultrafiltration membrane:
(1) Adding 0.5g of nano titanium dioxide into 60mL of toluene solvent, uniformly dispersing by ultrasonic, continuously adding 2mL of KH-550, reacting for 16h at 80 ℃, centrifuging, washing and drying the product to obtain aminated titanium dioxide;
(2) Dispersing 1g of aminated titanium dioxide in 50mL of ethanol solvent, adding silver nitrate solution containing 0.2g of silver nitrate, uniformly mixing, reacting for 1h at 20 ℃, adding 1.2g of sodium borohydride, continuously reacting for 1h, centrifuging, washing and drying to obtain silver-loaded titanium dioxide;
(3) Adding 0.06g of silver-carrying titanium dioxide into 0.05g of polyethylene glycol-1000 aqueous solution containing polyethylene glycol-1000, uniformly dispersing by ultrasonic, adding 1g of polyvinyl alcohol aqueous solution containing polyvinyl alcohol, uniformly stirring, defoaming for 6 hours, uniformly coating the casting solution on a glass plate by using a scraper, casting to form a film, standing at room temperature, and removing the film to obtain the polyvinyl alcohol-silver-carrying titanium dioxide composite ultrafiltration membrane.
Example 3
Preparation of a polyvinyl alcohol-silver-loaded titanium dioxide ultrafiltration membrane:
(1) Adding 0.5g of nano titanium dioxide into 60mL of toluene solvent, uniformly dispersing by ultrasonic, continuously adding 2mL of KH-550, reacting for 24 hours at 90 ℃, centrifuging, washing and drying the product to obtain aminated titanium dioxide;
(2) Dispersing 1g of aminated titanium dioxide in 50mL of ethanol solvent, adding silver nitrate solution containing 0.4g of silver nitrate, uniformly mixing, reacting for 2 hours at 30 ℃, adding 1.5g of sodium borohydride, continuously reacting for 2 hours, centrifuging, washing and drying to obtain silver-loaded titanium dioxide;
(3) Adding 0.1g of silver-carrying titanium dioxide into 0.08g of polyethylene glycol-1000 aqueous solution containing polyethylene glycol-1000, uniformly dispersing by ultrasonic, adding 1g of polyvinyl alcohol aqueous solution containing polyvinyl alcohol, uniformly stirring, defoaming for 10 hours, uniformly coating the casting solution on a glass plate by using a scraper, casting to form a film, standing at room temperature, and removing the film to obtain the polyvinyl alcohol-silver-carrying titanium dioxide composite ultrafiltration membrane.
Example 4
Preparation of chicken's gizzard-membrane extract:
Step S1: crushing chicken's gizzard-membrane, adding into deionized water with the weight being 10 times that of the chicken's gizzard-membrane, transferring into an ultrasonic instrument, continuously adding cellulase and neutral protein with the weight ratio of 1:2 as enzymes, setting the ultrasonic temperature to be 50 ℃, carrying out enzymolysis for 2 hours under the ultrasonic frequency condition of 300W, heating to 90 ℃ after the enzymolysis is finished, preserving heat for 10 minutes, inactivating the enzymes, filtering the solution after the solution is cooled, respectively mixing the separated filtrate and filter residues, concentrating and drying the filtrate to obtain chicken's gizzard-membrane enzyme extract powder;
step S2: adding the filter residue obtained in the step S1 into 4mL of hydrochloric acid solution with the concentration of 1%, leaching for 1h at 40 ℃, filtering after extraction, concentrating the filtrate, and vacuum drying to obtain chicken' S gizzard-membrane acid extract powder;
Step S3: mixing the powder materials in step S1 and step S2 thoroughly, and drying at 50deg.C to water content of 0.5% to obtain endothelium corneum Gigeriae Galli extract.
Example 5
Preparation of chicken's gizzard-membrane extract:
Step S1: crushing chicken's gizzard-membrane, adding into deionized water with the weight being 20 times that of the chicken's gizzard-membrane, transferring into an ultrasonic instrument, continuously adding cellulase and neutral protein with the weight ratio of 1:2 as enzymes, setting the ultrasonic temperature to 55 ℃, carrying out enzymolysis for 3 hours under the ultrasonic frequency condition of 360W, heating to 90 ℃ after the enzymolysis is finished, preserving heat for 20 minutes, inactivating the enzymes, filtering the solution after the solution is cooled, respectively mixing the separated filtrate and filter residues, concentrating and drying the filtrate to obtain chicken's gizzard-membrane enzyme extract powder;
Step S2: adding the filter residue obtained in the step S1 into 4mL of hydrochloric acid solution with the concentration of 1.5%, leaching for 2h at 45 ℃, filtering after extraction, concentrating the filtrate, and vacuum drying to obtain chicken' S gizzard-membrane acid extract powder;
step S3: mixing the powder materials in step S1 and step S2 thoroughly, and vacuum drying at 55deg.C until the water content is 1% to obtain endothelium corneum Gigeriae Galli extract.
Example 6
Preparation of chicken's gizzard-membrane extract:
Step S1: crushing chicken's gizzard-membrane, adding 30 times of deionized water, transferring into an ultrasonic instrument, continuously adding 2% of cellulase and neutral protein with the weight ratio of 1:2 as enzymes, setting the ultrasonic temperature to 60 ℃, carrying out enzymolysis for 4 hours under the ultrasonic frequency condition of 400W, heating to 90 ℃ after the enzymolysis is finished, preserving heat for 30 minutes, inactivating the enzymes, filtering the enzymes by using the polyvinyl alcohol-silver-loaded titanium dioxide ultrafiltration membrane prepared in the embodiment 2, respectively mixing the separated filtrate and filter residues, concentrating the filtrate, and drying to obtain chicken's gizzard-membrane enzyme extract powder;
Step S2: adding the filter residue obtained in the step S1 into 4mL of hydrochloric acid solution with the concentration of 2%, leaching for 3h at 50 ℃, filtering after extraction, concentrating the filtrate and drying to obtain chicken' S gizzard-membrane acid extract powder;
Step S3: mixing the powder materials in step S1 and step S2 thoroughly, and vacuum drying at 60deg.C to water content of 2% to obtain endothelium corneum Gigeriae Galli extract.
Example 7
The preparation of the haw and chicken's gizzard-skin chewable tablet comprises the following steps:
step SS1: drying and grinding 20g of hawthorn, 10g of sweet orange powder, 2g of microcrystalline cellulose and 5g of sodium alginate, and sieving with a 100-mesh sieve for later use;
Step SS2: 30g of sodium carboxymethyl cellulose is dissolved in deionized water to prepare suspension with the concentration of 8 percent;
Step SS3: 40g of the chicken's gizzard-membrane extract prepared in the embodiment 5 of the invention, 20g of hawthorn, 10g of sweet orange powder and 5g of sodium alginate are gradually and uniformly mixed, the mixture is added into the suspension in the step SS2, 1g of magnesium stearate is added while stirring, after uniform mixing, the mixture is sieved by a 20-mesh sieve, granulated and dried, and the mixture is tabletted by a tablet press, and is dried in vacuum until the water content is 1%, thus obtaining the hawthorn chicken's gizzard-membrane chewable tablet.
Example 8
The preparation of the haw and chicken's gizzard-skin chewable tablet comprises the following steps:
Step SS1: drying and grinding 25g of hawthorn, 20g of sweet orange powder, 5g of microcrystalline cellulose and 12g of sodium alginate, and sieving with a 100-mesh sieve for later use;
step SS2: 35g of sodium carboxymethyl cellulose is dissolved in deionized water to prepare suspension with the concentration of 10 percent;
Step SS3: 50g of the chicken's gizzard-membrane extract prepared in the embodiment 5 of the invention, 25g of hawthorn, 20g of sweet orange powder and 12g of sodium alginate are added in equal amounts and mixed uniformly, the mixture is added into the suspension in the step SS2, 1.5g of magnesium stearate is added while stirring, after uniform mixing, the mixture is sieved by a 20-mesh sieve, granulated and dried, and the mixture is tabletted by a tablet press, and vacuum-dried until the water content is 2%, thus obtaining the hawthorn chicken's gizzard-membrane chewable tablet.
Example 9
The preparation of the haw and chicken's gizzard-skin chewable tablet comprises the following steps:
Step SS1: drying 30g of hawthorn, 25g of sweet orange powder, 6g of microcrystalline cellulose and 15g of sodium alginate, grinding, and sieving with a 100-mesh sieve for later use;
step SS2: 50g of sodium carboxymethyl cellulose is dissolved in deionized water to prepare suspension with the concentration of 15%;
Step SS3: 60g of the chicken's gizzard-membrane extract prepared in the embodiment 5 of the invention, 30g of hawthorn, 25g of sweet orange powder and 15g of sodium alginate are added in equal amounts and mixed uniformly, the mixture is added into the suspension in the step SS2, 2g of magnesium stearate is added while stirring, after uniform mixing, the mixture is sieved by a 20-mesh sieve, granulated and dried, and the mixture is tabletted by a tablet press, and vacuum-dried until the water content is 3%, thus obtaining the hawthorn chicken's gizzard-membrane chewable tablet.
Antibacterial property test of polyvinyl alcohol-silver-loaded titanium dioxide ultrafiltration membrane prepared in examples 1 to 3 of the present invention:
the polyvinyl alcohol-silver-loaded titanium dioxide ultrafiltration membrane prepared in examples 1-3 of the present invention was cut into a round sample with a diameter of 10mm, which was added to a petri dish containing 1mL of E.coli suspension with a concentration of 1.5X10 8 CFU/mL, the petri dish was placed in a constant temperature incubator, and after culturing for 4 hours at 37℃the petri dish was taken out, and the diameter of the zone of inhibition was measured.
Testing the content and activity of the effective components in the chicken's gizzard-membrane extract prepared in the examples 4-6:
The chicken gizzard-membrane extracts prepared in examples 4-6 were tested for protein content using a coomassie brilliant blue-uv control assay: the endothelium corneum Gigeriae Galli extract prepared in examples 1-3 was dissolved in 1mL of deionized water, 5mL of Coomassie brilliant blue solution was added, mixed well, and after standing for 10min, the protein content was measured using a UV2550 type ultraviolet-visible spectrophotometer.
| Example 4 | Example 5 | Example 6 | |
| Protein content (%) | 5.1％ | 5.9％ | 6.2％ |
Polysaccharide content in the endothelium corneum Gigeriae Galli extracts prepared in examples 4-6 was tested using phenol-sulfuric acid colorimetry: and respectively sucking 0, 0.25, 0.50, 1.00, 1.50 and 2.00ml of glucose standard solution of 100ug/ml, placing into a 10ml test tube with a plug scale, continuously adding 1.0ml of phenol solution, uniformly mixing, rapidly adding 5.0ml of concentrated sulfuric acid, heating in a boiling water bath for 20min, cooling, performing colorimetric test on absorbance at 490nm, placing the prepared sample into the test tube, adding water to 10ml, measuring optical density according to the method, and calculating polysaccharide content.
| Example 4 | Example 5 | Example 6 | |
| Polysaccharide content (%) | 0.40％ | 0.51％ | 0.48％ |
The amino acid content of the chicken's gizzard-membrane extracts prepared in examples 4 to 6 was tested using an HPLC method.
| Example 4 | Example 5 | Example 6 | |
| Amino acid content (u/g) | 57.2 | 64.8 | 62.4 |
Protease activity in the chicken's gizzard-membrane extract prepared in 4-6 was tested using the Fu Lin Fen method: taking 0, 10, 20, 30, 40, 50 and 60ug/mL of standard amino acid solution respectively, adding 5.0mL of sodium carbonate solution with the concentration of 0.4mol/L into the standard amino acid solution, uniformly mixing, continuously adding 1.0mL of Fu Lin Fen reagent, uniformly mixing, preserving heat for 20min at the temperature of 40 ℃, measuring the light absorption value at the position of 660nm, and calculating a standard curve; the chicken's gizzard-membrane extract prepared in example 1-3 was prepared as an extract with a concentration of 1%, heated at 40℃for 5min, 1.0mL of the preheated complex protein solution was added, after 10min of reaction, 2mL of the trichloroacetic acid solution with a concentration of 0.4mol/L was added to terminate the reaction, filtration was performed, 1.0mL of the filtrate was taken, 5mL of the sodium carbonate solution with a concentration of 0.4mol/L and 1.0mL of the furin reagent were added to the filtrate, reaction was performed at 40℃for 20min, 2.0mL of the reaction solution was taken, absorbance was measured at 660nm, and protease activity was calculated under the condition of 40-fold dilution.
| Example 4 | Example 5 | Example 6 | |
| Protease activity (u/g) | 112.9 | 118.0 | 120.4 |
The efficacy test of the hawthorn chicken gizzard-membrane chewable tablets prepared in the embodiments 7-9 for promoting digestion and resolving food stagnation comprises the following steps:
Taking white rats as a study object, taking 50 male white rats weighing about 200g, randomly dividing into 4 experimental groups and 1 control group, 10 white rats in each group, carrying out continuous 7-day gastric lavage on the white rats in the experimental groups by using the hawthorn chicken's gizzard-membrane chewable tablets prepared in examples 7-9, carrying out gastric lavage on the white rats in the control group by using distilled water under the same condition, carrying out fasted 18h after the first day of drug lavage, separating the stomach, collecting the stomach content, centrifuging, taking 1mL of supernatant, adding 15mL of hydrochloric acid solution with the concentration of 0.005mol/L into a test tube, adding two protein tubes, sealing the mouth of the tubes, carrying out constant temperature culture for 24h at 37 ℃, measuring the lengths of transparent parts at two ends of the protein tubes, obtaining the average value, and counting the pepsin activity and the pepsin discharge amount.
| Example 7 | Example 8 | Example 8 | Control group | |
| Pepsin Activity (u/ml) | 449.0 | 459.2 | 462.7 | 342.8 |
| Pepsin discharge (u/h) | 341.9 | 352.1 | 349.5 | 152.4 |
The foregoing is merely illustrative and explanatory of the principles of the invention, as various modifications and additions may be made to the specific embodiments described, or similar thereto, by those skilled in the art, without departing from the principles of the invention or beyond the scope of the appended claims.
Claims (4)
1. The hawthorn chicken's gizzard-membrane chewable tablet is characterized by being prepared from the following raw materials in parts by weight: 20-30 parts of hawthorn, 40-60 parts of chicken's gizzard-membrane extract, 10-25 parts of sweet orange powder, 30-50 parts of sodium carboxymethyl cellulose, 2-6 parts of microcrystalline cellulose, 5-15 parts of sodium alginate and 1-2 parts of magnesium stearate;
The preparation method of the chicken's gizzard-membrane extract is as follows:
Step S1: crushing chicken's gizzard-membrane, adding into deionized water 10-30 times of the weight of the chicken's gizzard-membrane, transferring into an ultrasonic instrument, continuing to add complex enzyme for enzymolysis, heating to 90 ℃ after the enzymolysis is finished, preserving heat for 10-30min, inactivating the enzyme, filtering after cooling the solution, separating filtrate and filter residues, concentrating the filtrate, and drying to obtain chicken's gizzard-membrane enzyme extract powder;
Step S2: adding the filter residue obtained in the step S1 into 4mL of 1-2% hydrochloric acid solution, leaching at 40-50deg.C for 1-3h, filtering after extraction, concentrating the filtrate, and drying to obtain endothelium corneum Gigeriae Galli acid extract powder;
Step S3: fully mixing the powder materials in the step S1 and the step S2, and drying at 50-60 ℃ until the water content is 0.5-2% to obtain the chicken' S gizzard-membrane extract for later use;
the specific method for filtering in the step S1 and the step S2 is that the prepared solution is filtered by a polyvinyl alcohol-silver-loaded titanium dioxide micro-filtration membrane;
the preparation method of the polyvinyl alcohol-silver-loaded titanium dioxide micro-filtration membrane comprises the following steps:
(1) Carrying out surface modification on the nano titanium dioxide by using KH-550 to obtain aminated titanium dioxide;
(2) Dispersing the aminated titanium dioxide in an ethanol solvent, adding a silver nitrate solution, uniformly mixing, reacting for 1-3 hours at 10-30 ℃, adding sodium borohydride, continuously reacting for 1-2 hours, centrifuging, washing and drying to obtain silver-loaded titanium dioxide;
(3) Adding silver-carrying titanium dioxide into polyethylene glycol-1000 deionized water solution, uniformly dispersing by ultrasonic, adding polyvinyl alcohol aqueous solution, uniformly stirring, defoaming for 4-10 hours, uniformly coating a casting solution on a glass plate by using a scraper, casting to form a film, standing at room temperature, and removing the film to obtain a polyvinyl alcohol-silver-carrying titanium dioxide composite ultrafiltration film;
In the step (2), the weight ratio of the amination titanium dioxide to the silver nitrate to the sodium borohydride in the silver nitrate solution is 10:0.5-4:5-15;
In the step (3), silver-carrying titanium dioxide, polyethylene glycol in the polyethylene glycol-1000 aqueous solution and polyvinyl alcohol in the polyvinyl alcohol aqueous solution are in a weight ratio of 0.1-1:0.2-0.8:10.
2. The haw chicken' S gizzard-membrane chewable tablet according to claim 1, wherein the complex enzyme in the step S1 is cellulase and neutral protease in a weight ratio of 1:2.
3. The haw chicken' S gizzard-membrane chewable tablet according to claim 1, wherein the amount of the complex enzyme in the step S1 is 1-2%.
4. The haw chicken' S gizzard-membrane chewable tablet according to claim 1, wherein the enzymolysis in the step S1 is carried out at 50-60 ℃ with a frequency of 300-400W for 2-4h.
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