CN105779224A - High-bioactivity bee product health wine and preparation method thereof - Google Patents
High-bioactivity bee product health wine and preparation method thereof Download PDFInfo
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- CN105779224A CN105779224A CN201610171927.0A CN201610171927A CN105779224A CN 105779224 A CN105779224 A CN 105779224A CN 201610171927 A CN201610171927 A CN 201610171927A CN 105779224 A CN105779224 A CN 105779224A
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- 235000020795 whole food diet Nutrition 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
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Abstract
The invention discloses a high biological activity bee product health care wine, which takes queen bee larva or male bee pupa as a unique raw material, and adopts low-temperature processing technologies such as freezing, vacuum degassing, complex enzyme biological enzymolysis, vacuum centrifugation, biological demulsification, ultrasonic treatment, high-voltage pulse electric field treatment and the like in the whole process, thereby effectively improving the utilization rate of the queen bee larva or male bee pupa and reducing the loss of nutrition and health care substances. The ultrasonic wave and the peracetic acid are organically combined to accelerate aging of the base wine, the base wine is scientifically compounded with the queen bee larva or drone pupa hydrolysate, and high-voltage pulse aging is adopted again, so that oxidation of bioactive components of the queen bee larva hydrolysate by the peracetic acid is prevented, the aging effect of the bee product wine is improved, and the taste, stability and clarity of the bee product wine are improved. Finally, the clear and transparent bee product health care wine which has no fishy smell, strong stability, harmonious taste, mellowness, softness, lingering flavor, proper biological active substances, high nutritional value and good health care effect is prepared.
Description
Technical field
The present invention relates to the preparation of health promoting wine, be specifically related to a kind of high bioactivity bee product health promoting wine and preparation method thereof.
Background technology
" queen bee embryo " is also queen bee nit, claims " honeybee ", be the inevitable outcome in royal jelly and its product process in Chinese classical medical literature.Worker bee larvae about 1 age in days is moved in artificial Beequeen table by beekeeper, and worker bee is considered as the continuous feeding Lac regis apis of queen bee so that it is in 120 hours, volume increases by thousand times！Lac regis apis moistens it and grows up, owing to being in larva state, so intactly remaining various nutrient substance, by genetic analysis, queen bee embryo has acted on longevity and the objective reality reason thereof of queen bee, is in embryonism and more completely remains various nutrient substance, and ancient times is imperial palace tribute, its nutrition and medical value are superior to " Lac regis apis ", are the Nature magical energetic nourishing treasures of vouchsafing the mankind.In first pharmacopeia Shennong's Herbal of China, queen bee embryo is classified as top grade, " taste is put down, the nontoxic qi-restoratives that is slightly cold wins damaging the spleen and stomach, and clothes of a specified duration make us gloss, and color is not old, QI invigorating of making light of one's life by commiting suicide ".Supplement to the Herbal claims that " honeybee cures mainly erysipelas; rubella; stagnant heat in abdomen, profit defecation, breast enlargement juice; strong essence and blood; be full of body fluid ... " Mingyi Bielu " claims that " honeybee is made light of one's life by commiting suicide QI invigorating, controls shallow complexion, and menses are not smooth, female's soma is puckery, puerperal, weak after being ill ... " and Compendium of Material Medica thinks that " queen bee embryo " has effects such as " easing the affected liver nourish blood, kidney-tonifying and sperm-producing, happy face skin moistening, detoxification sore treatment, stimulating milk secretion of stimulating the menstrual flow, qi-restoratives YIN nourishing ".The main component of queen bee embryo has the element of Middle nutrition more than 50 that needed by human body is wanted; rich in protein; 18 seed amino acids; multivitamin; content such as vitamin B group, particularly vitamin D exceedes 10 times of cod-liver oil, goes back nearly 20 kinds of mineral and the trace element such as calcic, zinc, ferrum, copper, selenium, manganese, magnesium; superoxide dismutase (SOD), taurine etc..Modern medicine proves, queen bee nit has following effect: 1, improve nervous function: can eliminate insomnia and dreamful sleep, neurasthenia, Mental fatigue is forgetful, cardio palmus shape of breathing hard；2, endocrine regulation: queen bee embryo is nutritious, female incretion system can be regulated by nourishing the source ovary of women youth beauty, there is provided sufficient nutrient to ovary, delay the too early atrophy of ovary, safeguard its normal function, ovary is made to reach healthy state, thus solving the fatigue and weak that female endocrine dyscrasia causes, waist soreness, menoxenia, vaginal dryness, many symptoms such as sexual hypofunction；3, man's sperm count is few, that energy is poor, liquefying time extends patient is significantly improved, thus can be used for preventing and treating male sterility；4, can anti-ageing, beauty treatment, skin care, to pachylosis, wrinkle increases, chloasma, complexion are dark and gloomy, alopecia, disconnected sending out have clear improvement；5, enhancing development: queen bee embryo enhances metabolism, activated cell vigor, appetite stimulator, have additional nutrients, adult human body's void is weak, and lean body mass etc. all has great function；6, abundant acetylcholine is to malnutrition, hypoevolutism, brain healthy, recovery body constitution, has obvious effect；7, enhancing immunity: containing abundant natural taurine in queen bee embryo, to continuing to regulate human physiological functions, activated cell vigor, improving immunity of organisms has remarkable efficacy；8, digestive system: containing rich in protein in queen bee embryo, aminoacid, vitamin B1Etc. the synthesis to gastric juice and normal secretions can be promoted, enliven digestive system, treatment chronic gastritis, gastroduodenal ulcer have been had notable auxiliary therapeutic action.
Drone pupa is the honeybee work unfertilized egg at drone house property, hatches into larva, enters pupa time, the Apis body gathered in this phase is drone pupa, it forms to suck the growths such as Lac regis apis, Mel and pollen, has significantly high nutritive value, is one urgently wholefood leaved for development.According to scientific analysis, in drone pupa, the content of protein is higher than Lac regis apis, be pollen, more than 2 times of Lac Bovis seu Bubali, reach 33.4%, wherein, lysine content 4.45mg/100g, is 5 times of egg, and isoleucine content is 2.7%, exceedes other food.The content 16.08% of crude fat, wherein essential fatty acid content reaches 19.71%, is more than 2 times of pollen, beef, more than 3 times of egg, more than 10 times of Lac Bovis seu Bubali.The content of vitamin has exceeded beef, egg, higher 10 times than cod-liver oil.Additionally, drone pupa is possibly together with abundant mineral element, wherein the content of zinc is 2 times of Lac regis apis, is more than 10 times of pollen；The content of selenium is up to 2.4-2.8 μ g/100g.Visible, drone pupa is a nutrition treasure-house.Drone pupa does not only have good nutritive value, and has good pharmaceutical value.As far back as " figure is through book on Chinese herbal medicine ", " Book of Rites. inside then " etc. ancient books just has full and accurate record, in Shennong's Herbal, " honeybee is young " is considered as top grade, records the drug effect of " honeybee son's abnormal smells from the patient is sweet flat, is slightly cold nontoxic, cures mainly headache, and except poison, qi-restoratives is won "；Recording in " Mingyi Bielu ", honeybee son can cure mainly erysipelas, rubella, abdomen Nei Liure, profit defecation, go the effect of floating blood, lower milk, and anemia of pregnant woman, puerpera are had nourishing and therapeutical effect.In Asia, torrid areas, Africa, Australia and some nationalitys of South America have the custom of edible drone pupa also morning.Along with the development of science and technology, the understanding of drone pupa is had deeper of understanding by people.It is found that, living organism is had many-sided function by drone pupa, wherein promoting that body metabolism, growth promoter and decreased energy, muscle power weakness, malnutrition, Paederus fuscipes Curtis be slow, climacteric, geromorphism rehabilitation in comparatively prominent, in the diseases such as adjustment nervus centralis, endocrine regulation, digestive system, blood system, display that the curative effect of its uniqueness.
Prepare the more of health promoting wine with queen bee embryo or drone pupa for raw material, have directly by brewed to complete queen bee embryo and base liquor, such as: Chinese patent CN102618423A discloses a kind of Fengwang health-care wine, belongs to field of health care products.This health preserving wine formula is as follows, and rice wine: queen bee nit: Rhizoma Zingiberis Recens: Mel: essence=100: 1: 1: 2.5: 0.025, each component is with mass ratio range.Health care's effects such as this health preserving wine is suitable for adult and drinks, and has dispeling cold, whets the appetite, skin care, relaxing muscles and tendons to promote blood circulation.Chinese patent CN101899377A discloses a kind of queen bee wine and brewing method thereof.Wherein containing honeybee and Chinese liquor, the number of degrees of Chinese liquor are more than 50 degree.Described honeybee can be Apis, it is preferred to drone pupa, it is also possible to be honeybee series products, such as Lac regis apis.Wherein the ratio of various compositions is honeybee and/or honeybee series products: wine=1: 2～1: 10, is 1: 3～1: 7 preferably.Queen bee wine colourity brewed in the process of the present invention is purer, and mouthfeel is better.Chinese patent (ZL93100883.2) discloses a kind of queen cell high-efficient health-care tonic wine, and it is to mix 2.00% (weight) queen bee embryo in 86.00% (weight) wine base and to add 19 kinds of Chinese herbal medicine formulated through special process.This drinking utensils is improved body autoimmune, resist the disease and anti-ageing certain effect of waiting for a long time.In this wine, the composition containing Chinese herbal medicine is too high, taste of Chinese medicine mouthfeel weight, and consumer is difficult to accept, and is unfavorable for popularization and application.Chinese patent CN102787058B discloses a kind of drone pupa wine, and wherein, described drone pupa wine, according to parts by weight meter, is concocted by following component and formed: rice spirit 65-75；Pupa Apis 20-30；Radix Morindae Officinalis 2-6；Herba Cistanches 2-6；Fructus Lycii 1-3；Rhizoma Dioscoreae 0.5-1.5；Cortex Cinnamomi 0.5-1.5；Fructus Corni 0.5-1.5；Semen Cuscutae 0.5-1.5；Mel 3-7.Drone pupa wine provided by the present invention, with rice spirit for wine base, using edible animals and plants as being fragrant, taste compound, makes alcoholic drink by certain production and processing technology.Utilize the strengthening by means of tonics of drone pupa, the effect of QI invigorating of making light of one's life by commiting suicide, compatibility tonification essence and blood, the Chinese patent medicine of bone and muscle strengthening, add ultrasound wave and urge old technology, make this wine be deep succinum color and luster, limpid transparent, entrance is sweet, the not sudden and violent advantage such as not peppery, long times of aftertaste, and effect is notable.Patent the queen bee embryo disclosed above or effective ingredient-use rate of drone pupa is low, the loss of brew time length, bioactive ingredients is big, turbid appearance, has precipitation, has granulate queen bee embryo inside, affect appetite.
Also have queen bee embryo or drone pupa are ground, broken, such as: Chinese patent CN1376774A discloses a kind of queen bee foetus wine and production technology thereof, this wine is after namely the queen bee embryo extraction from bee colony is frozen with ultralow temperature is anxious, then through grinding, after uniform with base liquor 80-90% (weight) high efficient mixed for queen bee embryo 10-20% (weight), form then through configuration after ripening.This bartender deposits the active component of queen bee embryo and highlights, and enhancing immunity, strong able-bodied body, life lengthening, effect is notable.Chinese patent CN102776102B discloses a kind of health promoting wine containing bee product, and the weight portion of its raw material and raw material is: drone pupa is 1.0～1.5 weight portions, queen bee nit to be 2.0 weight portions, Lac regis apis be 0.02～0.04 weight portion, Radix Panacis Quinquefolii are 0.05～0.08 weight portion and the Chinese liquor of 50～70 weight portions；The preparation process of the health product that should contain bee product is as follows: 1) this drone pupa pulverizer or pulverizer are smashed；2) this queen bee nit pulverizer or pulverizer are smashed；3) this Radix Panacis Quinquefolii pulverizer or pulverizer are smashed；4) this drone pupa smashed, this queen bee nit smashed, this Radix Panacis Quinquefolii smashed and this Lac regis apis are put into storage tank, this Chinese liquor is added this storage tank simultaneously, then the opening lid of this storage tank is sealed, rock every day this storage tank several under, being filtered after one month, gained clear liquid is the health promoting wine containing bee product of the present invention.Although patent disclosed above improves the utilization rate of queen bee embryo, also shortening old taking time, but wine liquid is still muddy, with precipitation, bioactive ingredients still loses bigger.
In order to improve queen bee embryo or drone pupa health care colours of wine clarity, the carrying out the having processing methods such as filtration, ultrafiltration, freezing, such as: the preparation method that Chinese patent CN103710237B discloses a kind of bee wine, comprise the following steps: carry out being sufficiently stirred in proportion mixing with the high-quality Luzhou-flavor Tequ wine of 94%～98% (weight) by bee pollen 1%, Lac regis apis powder 0.5%, drone pupa powder 0.5%, queen bee embryo powder 0.5%, 5. other 0.2% (weight) respectively by fine powder shape raw material, carry out merceration and carry.After 15 days～20 days, fine straining (or ultrafiltration) after carrying out coarse filtration, fine straining, freezing respectively, store sth. in a cellar ripening, finished product.The bee product that formula is used by the present invention carries out accurate measurement preparation again after carrying out micronizing, ensure that qualitative, fixing quantity to product, evade traditional method uses and must use a large amount of antistaling agent during fresh goods, caused the quality to raw material, quantitatively not easily accurately slap.Although patent clarity disclosed above increases, but containing significant quantities of fat and protein in its bee product and adjuvant thereof, being cause muddy and precipitation basic reason, in the short time, wine liquid can be produced precipitation by muddiness oxidation, it does not have fundamentally solves outward appearance clarity problem.
Chinese patent CN103725742A discloses a kind of method that enzymatic isolation method prepares queen bee embryo peptide, including: sample pretreatment, queen bee nit powder defat, the preparation of queen bee larva sample liquid, ultrasonic Treatment queen bee larva sample liquid, queen bee larva sample liquid enzyme digestion reaction, enzymolysis solution is placed in boiling water bath 20 minutes enzyme denaturing of heating, it is cooled to room temperature, enzymolysis solution pH value is adjusted to 7.0, after centrifugal, takes the queen bee embryo Gly-His-Lys of supernatant, dry above-mentioned supernatant acquisition hydrolysis.The queen bee embryo polypeptide that the present invention prepares can be used in making the health product improving function of human body, and the more important thing is that screening obtains human body really effective biologically active polypeptide from the queen bee embryo polypeptide of hydrolysis.According to the processing method of queen bee embryo in patent disclosed above, be expected to be clarified, transparent queen bee embryo health promoting wine, but queen bee embryo will just can enzymolysis through lyophilizing, supercritical defat, operation is numerous and diverse, bioactive ingredients easily aoxidizes, degreasing effect is not ideal simultaneously, and introducing extracts organic solvent and there is food safety hidden danger.
Chinese patent CN103005466B discloses the application of a kind of drone pupae polypeptide extract, has the application in the food improving memory and/or anti-oxidation function including described drone pupae polypeptide extract as the food or preparation with improvement memory and/or anti-oxidation function；Described drone pupae polypeptide extract has the application in the health product improving memory and/or anti-oxidation function as the health product or preparation with improvement memory and/or anti-oxidation function；The application in preparation has the medicine improving memory and/or anti-oxidation function of the described drone pupae polypeptide extract.This drone pupae polypeptide extract significantly improves the function of memory and good antioxidation, is used as or prepares have the product improving memory and/or anti-oxidation function.Patent disclosed above adopts acetone to carry out defat, there is food safety hidden danger, and adopts mode of heating before enzymolysis, and raw material availability is low, and cost is high.
To sum up, with queen bee embryo or drone pupa be a kind of mouthfeel of unique raw material preparation coordinate, mellow, soft, long, local flavor is suitable, bioactive substance and be of high nutritive value, the bee product health promoting wine of clear that health-care effect is good has wide market prospect, is also the long-sought of those skilled in the art.
Summary of the invention
Solved by the invention technical problem is that the defect overcoming existing bee product health promoting wine, with queen bee embryo or drone pupa for raw material, first it is freezing, broken, vacuum outgas eliminating sargassum smell, enzymolysis obtains enzymolysis solution, separate through repeatedly traditional vacuum, breakdown of emulsion obtains not fatty and albumen hydrolyzed solution, hydrolyzed solution mixes with base liquor, ageing, it is filtrated to get a kind of mouthfeel with original queen bee embryo or drone pupa bioactive ingredients and local flavor to coordinate, mellow, soft, long, local flavor is suitable for, bioactive substance and being of high nutritive value, the bee product health promoting wine of the fishy smell without worm of the clear that health-care effect is good.
In order to achieve the above object, the present invention is by the following technical solutions:
The preparation method of a kind of high bioactivity bee product health promoting wine, comprises the following steps:
1) fresh queen bee embryo or drone pupa are mixed with solion 1:1.6-2.2 in mass ratio, in-18--22 DEG C of freezing 20-30min, broken, percentage of damage is 98-100%, broken thing particle diameter 200-300 μm, regulating broken thing pH value with lactic acid is 5-7, is warming up to 40-50 DEG C, is added thereto to fresh queen bee embryo or the compound enzyme of drone pupa quality 0.10-0.18%, mix homogeneously, in the degassed eliminating sargassum smell 10-15min of vacuum-0.07--0.09MPa, stand, continue enzymolysis 10-15min and obtain enzymolysis solution；
Further, described solion is the aqueous solution containing sodium ion 25-30mg/L, zinc ion 17-22mg/L, potassium ion 15-20mg/L, calcium ion 12-14mg/L and magnesium ion 9-11mg/L；
Further, described compound enzyme quality group becomes: flavor protease: acid protease: lipase=2-4:1-3:0.3-0.5；
null2) enzymolysis solution is put 5000-7000r/min traditional vacuum 8-10min in vacuum centrifuge，Separate from top to bottom and obtain free-fat、Emulsion、Hydrolyzed solution and residue，The emulsion obtained is controlled temperature and is 45-55 DEG C，Add the biological demulsifying agent breakdown of emulsion 20-40min of emulsion quality 0.08-0.12%,In 4000-6000r/min traditional vacuum 2 times after breakdown of emulsion，Interval time 1-3h，Centrifugal 10-15min every time，Merge three centrifugal hydrolyzed solutions obtained，Take the Ovum Gallus domesticus album of its quality 0.5-0.7%，Put in blender and stir 10-12min in rotating speed 800-1000r/min,Until Ovum Gallus domesticus album all becomes foam and obtains egg white foams，Egg white foams is added and merges in the hydrolyzed solution obtained，Normal pressure boils 5-8min,It is down to room temperature，In 5000-7000r/min traditional vacuum 8-10min，Collect hydrolyzed solution，Filter through kieselguhr successively、Namely ultrafiltration obtains transparent、Stable queen bee embryo or drone pupa hydrolyzed solution；
Further, described traditional vacuum condition is temperature 6-10 DEG C, vacuum-0.02--0.04MPa；
Further, described biological demulsifying agent is that glycolipid class, lipopeptid class, cell wall are in conjunction with the one or more combination in class biological demulsifying agent；
Preferably, the quality group of described biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall is in conjunction with class=5-7:4-6:2-4；
Preferably, described glycolipid class biological demulsifying agent is one or both combinations in rhamnolipid, alkyl polyglucoside；
It is highly preferred that the quality group of described glycolipid class biological demulsifying agent becomes: rhamnolipid: alkyl polyglucoside=3-5:1-2；
3) queen bee embryo or the base liquor of drone pupa hydrolyzed solution quality 1-3 times are taken, it is added thereto to peracetic acid soln, making peroxyacetic acid concentration is 400-480ppm, Homogeneous phase mixing, in power 200-400W, carries out ultrasonic Treatment 5-10min when frequency 30-40KHz, treated base liquor and queen bee embryo or drone pupa hydrolyzed solution Homogeneous phase mixing, control mixed liquor temperature and be 25-35 DEG C, then pass through continuous high voltage pulse process chamber and process, stand, filter and obtain high bioactivity bee product health promoting wine；
Further, described base liquor is 40-60 degree Chinese liquor；
Further, the mass percent concentration of described peracetic acid soln is 10-30%；
Further, described high voltage pulse electric field processing condition is electric field intensity 25-30kV/cm, umber of pulse 40-50.
Another object of the present invention is to provide the high bioactivity bee product health promoting wine prepared through said method.
Beneficial effect:
The present invention is with queen bee embryo or drone pupa for unique raw material, the low temperature processing techniques such as omnidistance employing freezing, vacuum outgas, compound enzyme biological enzymolysis, traditional vacuum, biological demulsifying, supersound process, high voltage pulse electric field processing, it is effectively increased the utilization rate of queen bee embryo or drone pupa, reducing the loss of nutrition and health substance, the bioactive ingredients remaining queen bee embryo to greatest extent becomes content of material with nutrition.Vacuum outgas is organically combined with compound enzyme biological enzymolysis, not only reduce the dissolved oxygen content of enzymolysis solution, effectively prevent bioactive ingredients oxidation deterioration, and eliminate the worm fishy smell of queen bee nit or drone pupa in enzymolysis solution, improve the mouthfeel of health promoting wine, add appetite, solion is composite with the science of queen bee embryo and compound enzyme, compound enzyme vigor is played maximum, catalytic capability is more thorough, and then the protein in queen bee embryo and fat are fully degraded, improve stability and the clarity of queen bee foetus wine, wherein generation fatty acid and cation also can remarkably promote the Chen Hua of health promoting wine；Biological demulsifying agent breakdown of emulsion and traditional vacuum are organically combined, the free-fat in enzymolysis solution and hydrolyzed solution can be made to be sufficiently separated, obtain nutrition and bioactive substance content is abundant, the hydrolyzed solution of clear, add egg white foams to add and to boil enzyme denaturing, queen bee embryo or drone pupa hydrolyzed solution more stable, transparent；By ultrasound wave and urge old auxiliary agent (peracetic acid) organically combine urge old base liquor, composite with queen bee embryo or drone pupa hydrolyzed solution science, it is again with high-voltage pulse and urges old, both the peracetic acid oxidation to queen bee embryo hydrolyzed solution bioactive ingredients had been prevented, improve again the Chen Hua effect of queen bee foetus wine, improve queen bee embryo or the mouthfeel of drone pupa wine, stability and clarity.Combining in order and common collaborative by above-mentioned every technical scheme, final prepare a kind of without worm fishy smell, stability is strong, mouthfeel is coordinated, mellow, soft, long, local flavor is suitable for, bioactive substance and be of high nutritive value, the bee product health promoting wine of clear that health-care effect is good.Concrete test effect is shown in embodiment six to seven.
It should be noted that the health-care effect of high bioactivity bee product health promoting wine of the present invention is the result that each step technique feature is mutually worked in coordination with, interacted, the not continuity of simple step and technical characteristic superposition, the combination of each step technique feature and the collaborative effect produced, considerably beyond the superposition of each monotechnics feature functionality and effect, have advanced preferably and practicality.
Detailed description of the invention
The present invention is described below by specific embodiment.Unless stated otherwise, technological means used in the present invention is method known in those skilled in the art.It addition, embodiment is interpreted as illustrative, and unrestricted the scope of the present invention, the spirit and scope of the invention are limited only by the claims that follow.To those skilled in the art, under the premise without departing substantially from spirit and scope of the present invention, the various changes or the changes that carry out the material component in these embodiments and consumption fall within protection scope of the present invention.
Embodiment one:
A kind of high bioactivity queen bee embryo health promoting wine, its preparation method comprises the following steps:
1) fresh queen bee embryo is mixed with solion 1:1.8 in mass ratio, in-20 DEG C of freezing 25min, broken, percentage of damage is 99%, broken thing particle diameter 250 μm, regulating broken thing pH value with lactic acid is 6, is warming up to 45 DEG C, is added thereto to the compound enzyme of fresh queen bee embryo quality 0.14%, mix homogeneously, in the degassed eliminating sargassum smell 13min of vacuum-0.08MPa, stand, continue enzymolysis 13min and obtain enzymolysis solution；
Above-mentioned solion is the aqueous solution containing sodium ion 28mg/L, zinc ion 20mg/L, potassium ion 18mg/L, calcium ion 13mg/L and magnesium ion 10mg/L；
Above-mentioned compound enzyme quality group becomes: flavor protease: acid protease: lipase=3:2:0.4；
null2) enzymolysis solution is put in vacuum centrifuge,6000r/min traditional vacuum 9min，Separate from top to bottom and obtain free-fat、Emulsion、Hydrolyzed solution and residue，The emulsion obtained is controlled temperature is 50 DEG C，Add the biological demulsifying agent breakdown of emulsion 30min of emulsion quality 0.10%,In 5000r/min traditional vacuum 2 times after breakdown of emulsion，Interval time 2h，Centrifugal 13min every time，Merge three centrifugal hydrolyzed solutions obtained，Take the Ovum Gallus domesticus album of its quality 0.6%，Put in blender and stir 11min in rotating speed 900r/min,Until Ovum Gallus domesticus album all becomes foam and obtains egg white foams，Egg white foams is added and merges in the hydrolyzed solution obtained，Normal pressure boils 7min,It is down to room temperature，In 6000r/min traditional vacuum 9min，Collect hydrolyzed solution，Filter through kieselguhr successively、Namely ultrafiltration obtains transparent、Stable queen bee embryo hydrolyzed solution；
Above-mentioned traditional vacuum condition is temperature 8 DEG C, vacuum-0.03MPa；
The quality group of above-mentioned biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall is in conjunction with class=6:5:3
Above-mentioned glycolipid class biological demulsifying agent quality group becomes: rhamnolipid: alkyl polyglucoside=4:1.5；
3) base liquor of queen bee embryo hydrolyzed solution quality 2 times is taken, it is added thereto to peracetic acid soln, making peroxyacetic acid concentration is 440ppm, Homogeneous phase mixing, in power 300W, carries out ultrasonic Treatment 8min when frequency 35KHz, treated base liquor and queen bee embryo hydrolyzed solution Homogeneous phase mixing, controlling mixed liquor temperature is 30 DEG C, then passes through continuous high voltage pulse process chamber and processes, stands, filters and obtain high bioactivity queen bee embryo health promoting wine；
Above-mentioned base liquor is 50 degree of Chinese liquor；
The mass percent concentration of above-mentioned peracetic acid soln is 20%；
Above-mentioned high voltage pulse electric field processing condition is electric field intensity 28kV/cm, umber of pulse 45.
Embodiment two:
A kind of high bioactivity queen bee embryo health promoting wine, its preparation method comprises the following steps:
1) fresh queen bee embryo is mixed with solion 1:1.6 in mass ratio, in-18 DEG C of freezing 20min, broken, percentage of damage is 98%, broken thing particle diameter 200 μm, regulating broken thing pH value with lactic acid is 5, is warming up to 40 DEG C, is added thereto to the compound enzyme of fresh queen bee embryo quality 0.10%, mix homogeneously, in the degassed eliminating sargassum smell 10min of vacuum-0.07MPa, stand, continue enzymolysis 10min and obtain enzymolysis solution；
Above-mentioned solion is the aqueous solution containing sodium ion 25mg/L, zinc ion 17mg/L, potassium ion 15mg/L, calcium ion 12mg/L and magnesium ion 9mg/L；
Above-mentioned compound enzyme quality group becomes: flavor protease: acid protease: lipase=2:1:0.3；
null2) enzymolysis solution is put 5000r/min traditional vacuum 8min in vacuum centrifuge，Separate from top to bottom and obtain free-fat、Emulsion、Hydrolyzed solution and residue，The emulsion obtained is controlled temperature is 45 DEG C，Add the biological demulsifying agent breakdown of emulsion 20min of emulsion quality 0.08%,In 4000r/min traditional vacuum 2 times after breakdown of emulsion，Interval time 1h，Centrifugal 10min every time，Merge three centrifugal hydrolyzed solutions obtained，Take the Ovum Gallus domesticus album of its quality 0.5%，Put in blender and stir 10min in rotating speed 800r/min,Until Ovum Gallus domesticus album all becomes foam and obtains egg white foams，Egg white foams is added and merges in the hydrolyzed solution obtained，Normal pressure boils 5min,It is down to room temperature，In 5000r/min traditional vacuum 8min，Collect hydrolyzed solution，Filter through kieselguhr successively、Namely ultrafiltration obtains transparent、Stable queen bee embryo hydrolyzed solution；
Above-mentioned traditional vacuum condition is temperature 6 DEG C, vacuum-0.02MPa；
Above-mentioned biological demulsifying agent is glycolipid class biological demulsifying agent, and it is specially rhamnolipid；
3) base liquor of queen bee embryo hydrolyzed solution quality 1 times is taken, it is added thereto to peracetic acid soln, making peroxyacetic acid concentration is 400ppm, Homogeneous phase mixing, in power 200W, carries out ultrasonic Treatment 5min when frequency 30KHz, treated base liquor and queen bee embryo hydrolyzed solution Homogeneous phase mixing, controlling mixed liquor temperature is 25 DEG C, then passes through continuous high voltage pulse process chamber and processes, stands, filters and obtain high bioactivity queen bee embryo health promoting wine；
Above-mentioned base liquor is 40 degree of Chinese liquor；
The mass percent concentration of above-mentioned peracetic acid soln is 10%；
Above-mentioned high voltage pulse electric field processing condition is electric field intensity 25kV/cm, umber of pulse 40.
Embodiment three:
A kind of high bioactivity queen bee embryo health promoting wine, its preparation method comprises the following steps:
1) fresh queen bee embryo is mixed with solion 1:2.2 in mass ratio, in-22 DEG C of freezing 30min, broken, percentage of damage is 100%, broken thing particle diameter 300 μm, regulating broken thing pH value with lactic acid is 7, is warming up to 50 DEG C, is added thereto to the compound enzyme of fresh queen bee embryo quality 0.18%, mix homogeneously, in the degassed eliminating sargassum smell 15min of vacuum-0.09MPa, stand, continue enzymolysis 15min and obtain enzymolysis solution；
Above-mentioned solion is the aqueous solution containing sodium ion 30mg/L, zinc ion 22mg/L, potassium ion 20mg/L, calcium ion 14mg/L and magnesium ion 11mg/L；
Above-mentioned compound enzyme quality group becomes: flavor protease: acid protease: lipase=4:3:0.5；
null2) enzymolysis solution is put 7000r/min traditional vacuum 10min in vacuum centrifuge，Separate from top to bottom and obtain free-fat、Emulsion、Hydrolyzed solution and residue，The emulsion obtained is controlled temperature is 55 DEG C，Add the biological demulsifying agent breakdown of emulsion 40min of emulsion quality 0.12%,In 6000r/min traditional vacuum 2 times after breakdown of emulsion，Interval time 3h，Centrifugal 15min every time，Merge three centrifugal hydrolyzed solutions obtained，Take the Ovum Gallus domesticus album of its quality 0.7%，Put in blender and stir 12min in rotating speed 1000r/min,Until Ovum Gallus domesticus album all becomes foam and obtains egg white foams，Egg white foams is added and merges in the hydrolyzed solution obtained，Normal pressure boils 8min,It is down to room temperature，In 7000r/min traditional vacuum 10min，Collect hydrolyzed solution，Filter through kieselguhr successively、Namely ultrafiltration obtains transparent、Stable queen bee embryo hydrolyzed solution；
Above-mentioned traditional vacuum condition is temperature 10 DEG C, vacuum-0.04MPa；
The quality group of above-mentioned biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall is in conjunction with class=6:5:3；
3) base liquor of queen bee embryo hydrolyzed solution quality 3 times is taken, it is added thereto to peracetic acid soln, making peroxyacetic acid concentration is 480ppm, Homogeneous phase mixing, in power 400W, carries out ultrasonic Treatment 10min when frequency 40KHz, treated base liquor and queen bee embryo hydrolyzed solution Homogeneous phase mixing, controlling mixed liquor temperature is 35 DEG C, then passes through continuous high voltage pulse process chamber and processes, stands, filters and obtain high bioactivity queen bee embryo health promoting wine；
Above-mentioned base liquor is 60 degree of Chinese liquor；
The mass percent concentration of above-mentioned peracetic acid soln is 30%；
Above-mentioned high voltage pulse electric field processing condition is electric field intensity 30kV/cm, umber of pulse 50.
Embodiment four:
A kind of high bioactivity drone pupa health promoting wine, its preparation method comprises the following steps:
1) fresh drone pupa is mixed with solion 1:2.0 in mass ratio, in-19 DEG C of freezing 23min, broken, percentage of damage is 98%, broken thing particle diameter 230 μm, regulating broken thing pH value with lactic acid is 5.5, is warming up to 43 DEG C, is added thereto to the compound enzyme of fresh drone pupa quality 0.12%, mix homogeneously, in the degassed eliminating sargassum smell 12min of vacuum-0.07MPa, stand, continue enzymolysis 12min and obtain enzymolysis solution；
Above-mentioned solion is the aqueous solution containing sodium ion 27mg/L, zinc ion 19mg/L, potassium ion 17mg/L, calcium ion 12mg/L and magnesium ion 9mg/L；
Above-mentioned compound enzyme quality group becomes: flavor protease: acid protease: lipase=2:1:0.3；
null2) enzymolysis solution is put 5500r/min traditional vacuum 8min in vacuum centrifuge，Separate from top to bottom and obtain free-fat、Emulsion、Hydrolyzed solution and residue，The emulsion obtained is controlled temperature is 48 DEG C，Add the biological demulsifying agent breakdown of emulsion 25min of emulsion quality 0.09%,In 4500r/min traditional vacuum 2 times after breakdown of emulsion，Interval time 1.5h，Centrifugal 12min every time，Merge three centrifugal hydrolyzed solutions obtained，Take the Ovum Gallus domesticus album of its quality 0.5%，Put in blender and stir 10min in rotating speed 850r/min,Until Ovum Gallus domesticus album all becomes foam and obtains egg white foams，Egg white foams is added and merges in the hydrolyzed solution obtained，Normal pressure boils 6min,It is down to room temperature，In 5500r/min traditional vacuum 8min，Collect hydrolyzed solution，Filter through kieselguhr successively、Namely ultrafiltration obtains transparent、Stable drone pupa hydrolyzed solution；
Upper traditional vacuum condition is temperature 8 DEG C, vacuum-0.02MPa；
Above-mentioned biological demulsifying agent is lipopeptid class biological demulsifying agent；
3) base liquor of drone pupa hydrolyzed solution quality 1 times is taken, it is added thereto to peracetic acid soln, making peroxyacetic acid concentration is 420ppm, Homogeneous phase mixing, in power 250W, carries out ultrasonic Treatment 7min when frequency 30KHz, treated base liquor and drone pupa hydrolyzed solution Homogeneous phase mixing, controlling mixed liquor temperature is 28 DEG C, then passes through continuous high voltage pulse process chamber and processes, stands, filters and obtain high bioactivity drone pupa health promoting wine；
Above-mentioned base liquor is 45 degree of Chinese liquor；
The mass percent concentration of above-mentioned peracetic acid soln is 15%；
Above-mentioned high voltage pulse electric field processing condition is electric field intensity 27kV/cm, umber of pulse 43.
Embodiment five:
A kind of high bioactivity drone pupa health promoting wine, its preparation method comprises the following steps:
1) fresh drone pupa is mixed with solion 1:1.7 in mass ratio, in-21 DEG C of freezing 28min, broken, percentage of damage is 99%, broken thing particle diameter 280 μm, regulating broken thing pH value with lactic acid is 6.5, is warming up to 48 DEG C, is added thereto to the compound enzyme of fresh drone pupa quality 0.16%, mix homogeneously, in the degassed eliminating sargassum smell 14min of vacuum-0.09MPa, stand, continue enzymolysis 14min and obtain enzymolysis solution；
Above-mentioned solion is the aqueous solution containing sodium ion 29mg/L, zinc ion 21mg/L, potassium ion 19mg/L, calcium ion 14mg/L and magnesium ion 11mg/L；
Above-mentioned compound enzyme quality group becomes: flavor protease: acid protease: lipase=3.5:2.5:0.4；
null2) enzymolysis solution is put 6500r/min traditional vacuum 10min in vacuum centrifuge，Separate from top to bottom and obtain free-fat、Emulsion、Hydrolyzed solution and residue，The emulsion obtained is controlled temperature is 53 DEG C，Add the biological demulsifying agent breakdown of emulsion 35min of emulsion quality 0.11%,In 5500r/min traditional vacuum 2 times after breakdown of emulsion，Interval time 2.5h，Centrifugal 14min every time，Merge three centrifugal hydrolyzed solutions obtained，Take the Ovum Gallus domesticus album of its quality 0.7%，Put in blender and stir 12min in rotating speed 950r/min,Until Ovum Gallus domesticus album all becomes foam and obtains egg white foams，Egg white foams is added and merges in the hydrolyzed solution obtained，Normal pressure boils 7min,It is down to room temperature，In 6500r/min traditional vacuum 10min，Collect hydrolyzed solution，Filter through kieselguhr successively、Namely ultrafiltration obtains transparent、Stable drone pupa hydrolyzed solution；
Above-mentioned traditional vacuum condition is temperature 9 DEG C, vacuum-0.04MPa；
Above-mentioned biological demulsifying agent is that cell wall is in conjunction with class biological demulsifying agent；
3) base liquor of drone pupa hydrolyzed solution quality 3 times is taken, it is added thereto to peracetic acid soln, making peroxyacetic acid concentration is 460ppm, Homogeneous phase mixing, in power 350W, carries out ultrasonic Treatment 9min when frequency 40KHz, treated base liquor and drone pupa hydrolyzed solution Homogeneous phase mixing, controlling mixed liquor temperature is 33 DEG C, then passes through continuous high voltage pulse process chamber and processes, stands, filters and obtain high bioactivity drone pupa health promoting wine；
Above-mentioned base liquor is 55 degree of Chinese liquor；
The mass percent concentration of above-mentioned peracetic acid soln is 25%；
Above-mentioned high voltage pulse electric field processing condition is electric field intensity 29kV/cm, umber of pulse 48.
The sensory evaluation test of embodiment six queen bee embryo health promoting wine of the present invention
The queen bee embryo health promoting wine that the embodiment of the present invention 1 is prepared by 24 personnel is invited to judge with the queen bee embryo health promoting wine of commercially available two kinds of similar identical dates of manufacture, sense organ is given a mark, wherein specialty and each 12 of layman, professional is young, middle aged, each 4 of old age, men and women half and half, layman is juvenile, young, middle aged, each 3 of old age, and men and women half and half；Marking includes outward appearance (20 points), quality (25 points), local flavor (30 points), four aspects of mouthfeel (25 points), and marking personnel independently carry out, and are independent of each other, and judges result with guarantee accurate.Having added up judging result, equal score value takes approximation, retains integer, is specifically shown in table 1:
Table 1: sensory evaluation statistical result
Note: show significant difference (P < 0.05) with the different lowercase alphabet of mark in a line, the different capitalization of mark represents difference extremely notable (P < 0.01), indicates same letter and represents difference not notable (P > 0.05).
Result above shows, any one will be substantially better than commercially available queen bee embryo health promoting wine to queen bee embryo health promoting wine prepared by the present invention from outward appearance, quality, local flavor and mouthfeel, particularly outward appearance clarity, local flavor and mouthfeel are fabulous, also are adapted for different age group, the consumer of different hierarchy of consumption eats simultaneously.
It should be understood that queen bee embryo health promoting wine prepared by embodiment of the present invention 2-3 has above-mentioned experiment effect equally, between each embodiment and little with above-mentioned experiment effect diversity.
The impact on immunity of organisms of the embodiment seven queen bee embryo health promoting wine of the present invention
1 experiment purpose
Test (mouse forced swimming) by exercise tolerance, verify the raising immunity of queen bee embryo health promoting wine of the present invention, antifatigue effect.
2 experiment materials and reagent
2.1 for reagent thing:
Commercially available queen bee embryo health promoting wine (G1)；Commercially available queen bee embryo health promoting wine (G2)；Queen bee embryo health promoting wine (G3-G5) prepared by embodiment of the present invention 1-3.
2.2 reagent:
Liver/muscle glycogen testing cassete, builds up institute of biological products purchased from Nanjing；Concentrated sulphuric acid (AR), Nanjing Chemistry Reagent Co., Ltd.；Normal saline, Shangdong Changfu Jiejing Pharmaceutical Industry Co., Ltd..
3. laboratory animal
ICR mice, ♂, cleaning grade, body weight 18-22g, Ningxia Medical University's comparative medicine center provide, the free diet of mice during experiment.
4. key instrument
Aluminum swimming trunk (50cm × 50cm × 40cm), galvanized wire, low-temperature and high-speed centrifuge: 5804R type, Eppendrof company；Water-bath: DK-S26 type, the grand experimental facilities company limited of upper Nereid；Electronic scale: BS224S type, Sartorius company；Stopwatch, thermometer
5. experiment packet
5.1 dosage packets and given the test agent give the time and at random mice are divided into 6 groups, often group 10,1st group to the 5th group medicine giving G1～G5 respectively, 6th group is blank group, give isopyknic distilled water, the often every average daily gavage of group 1 time, gavage volume is 0.2ml/10g, gives given the test agent continuously 30 days.
5.2 sample preparations the 1st group to the 7th group: weigh 2.25g drug sample, be assigned to 150ml with distilled water；Blank group: distilled water 150ml.
6. experimental technique
After 6.1 swimming with a load attached to the body experiment last administration 30min, putting mice in swimming trunk, the depth of water is no less than 30cm, water temperature 25 ± 1 DEG C, the sheet lead of rat-tail root load 5% body weight, and record mice swimming started to the dead time, as mice swimming time.
After 6.2 mice serum carbamide measure last administration 30min, not swimming with a load attached to the body 90min in the water that temperature is 30 DEG C, eyeball blood sampling 0.5mL (being not added with anticoagulant) is plucked after rest 60min, put 4 DEG C of refrigerator 3h, after hemopexis, the centrifugal 15min of 2000r/min, takes serum and send clinical laboratory of Affiliated Hospital of Ningxia Medical University to detect.
After the mensuration last administration 30min of 6.3 hepatic glycogen, not swimming with a load attached to the body 90min in the water that temperature is 25 ± 1 DEG C, cervical dislocation puts to death mice, clean with normal saline, and with, after filter paper suck dry moisture, accurately weighing liver 100mg, hepatic glycogen detection kit detection Mouse Liver glycogen content.
Blood sampling after the mensuration last administration 30min of 6.4 blood lactase acid, does not then bear a heavy burden and stops after the water went swimming 10min that temperature is 30 DEG C.Lactic acid instrument assay method: respectively before swimming, each blood sampling 20 μ L add in 40 μ L rupture of membranes liquid after rest 20min after swimming, after swimming, fully vibration smudge cells lactic acid instrument measures immediately.(blood lactase acid area under curve=5 × (after front blood lactase acid value+3 × swimming of swimming the blood lactase acid value of 20min after blood lactase acid value+2 × swimming of 0min)
7. observation index walking weight load, blood lactase acid, carbamide, glycogen initial value
8. statistical method experimental data x ± s represents, adopts t inspection to compare between organizing
9. experimental result
The impact on Mouse Weight of the 9.1 queen bee embryo health promoting wine of the present invention
Each group mice is after giving G1～G5 medicine, before, in, post-weight is shown in shown in following table respectively, and each original body mass organizing mice and weightening finish body weight compare equal no difference of science of statistics (P > 0.05) with matched group, it was shown that G1～G5 medicine is all without obvious toxicity.Experimental result refers to table 2.
The original body mass of table 2 swimming with a load attached to the body experiment mice, mid-term body weight and terminate body weight
The impact on the mice burden swimming time of the 9.2 queen bee embryo health promoting wine of the present invention
After per os gives mice G1～G5 medicine, G1～G2 medicine compares with blank group, the mice burden swimming time can be obviously prolonged, there is significant difference (P < 0.05), queen bee embryo health promoting wine G3～G5 medicine of the present invention compares with blank group, can significantly extend the mice burden swimming time, there is pole significant difference (P < 0.01), and be substantially better than G1～G2 medicine.The results detailed in Table 3.
The impact on the mice burden swimming time of the table 3 queen bee embryo health promoting wine
" * " p < 0.05vs blank；
" * * " p < 0.01vs blank；
9.3 queen bee embryo health promoting wine of the present invention are on the impact of blood lactase acid before and after mouse movement
After per os gives the queen bee embryo health promoting wine of the mice present invention, blood lactase acid area under curve after mouse movement is compared by queen bee embryo health promoting wine G3～G5 medicine of the present invention with matched group significant difference (P < 0.05), decrease though G1～G2 medicine group Mouse Blood lactic acid area under curve compares with matched group, but and no difference of science of statistics (P > 0.05).Result is in Table 4.
Table 4 queen bee embryo health promoting wine of the present invention is on the impact of blood lactase acid level before and after mouse movement
" * " p < 0.05vs blank；
The impact on Mouse Liver glycogen of the 9.4 queen bee embryo health promoting wine of the present invention
After per os gives mice G1～G5 medicine, G1～G2 medicine compares with blank group, Mouse Liver glycogen content all has obvious rising, there is significant difference (P < 0.05), queen bee embryo health promoting wine G3～G5 medicine of the present invention compares with blank group, Mouse Liver glycogen content all has obvious rising, has pole significant difference (P < 0.01), and is substantially better than G1～G2 medicine.The results detailed in Table 5.
The impact on Mouse Liver glycogen content of the table 5 queen bee embryo health promoting wine of the present invention
" * " p < 0.05vs blank；
" * * " p < 0.01vs blank；
The impact on mice serum carbamide of the 9.5 queen bee embryo health promoting wine of the present invention
After per os gives mice G1～G5 medicine, G1～G2 medicine group compares with blank group, after mouse movement, serum urea content all has obvious reduction, there is significant difference (P < 0.05), queen bee embryo health promoting wine G3～G5 medicine of the present invention compares with blank group, after mouse movement, serum urea content all has obvious reduction, has pole significant difference (P < 0.01), and is substantially better than G1～G2 medicine.The results detailed in Table 6.
The impact on mice serum urea content of the table 6 queen bee embryo health promoting wine of the present invention
" * " p < 0.05vs blank；
" * * " p < 0.01vs blank；
10. experiment conclusion
This experiment is tested mainly through mice burden swimming, and the deposit simultaneously detecting Mouse Liver glycogen observes the effect of queen bee embryo health promoting wine of the present invention raising immunity, resisting fatigue.Preliminary Results shows below:
1, G3～G5 queen bee embryo health promoting wine of the present invention all can extend the mice burden swimming time (P < 0.01), and effect is substantially better than the queen bee embryo health promoting wine of other G1～G2.
2, biochemistry detection aspect shows, the each dosage group of G3～G5 queen bee embryo health promoting wine of the present invention all can reduce move after lactic acid content produced by glucose anerobic glycolysis in mice serum, compare with matched group and have significant difference (P < 0.05), and although the queen bee embryo health promoting wine of other G1～G2 also can reduce after motion lactic acid content produced by glucose anerobic glycolysis in mice serum, but compare with matched group, no difference of science of statistics (P > 0.05)；
3, each dosage group of G3～G5 queen bee embryo health promoting wine of the present invention all can significantly improve the deposit (P < 0.01) of glycogen in mouse liver, and effect is substantially better than the queen bee embryo health promoting wine of other G1～G2；
4, metabolic arthritis model finds, G3～G5 queen bee embryo health promoting wine of the present invention can significantly reduce the content (P < 0.01) of urea in serum after mice is swum, and effect is substantially better than other G1～G2 queen bee embryo health promoting wine；
11. conclusion
Above-mentioned experiment proves that queen bee embryo health promoting wine of the present invention can significantly improve immunity of organisms, improve muscle power and the endurance of mice, the content of urea in serum and lactic acid after reduction mouse movement, and the deposit of glycogen in mouse liver can be significantly improved, contribute to alleviating the fatigue that sports load causes；The time that mice burden swimming to power exhausts can be extended.
Claims (10)
1. the preparation method of a high bioactivity bee product health promoting wine, it is characterised in that comprise the following steps:
1) fresh queen bee embryo or drone pupa are mixed with solion 1:1.6-2.2 in mass ratio, in-18--22 DEG C of freezing 20-30min, broken, percentage of damage is 98-100%, broken thing particle diameter 200-300 μm, regulating broken thing pH value with lactic acid is 5-7, is warming up to 40-50 DEG C, is added thereto to fresh queen bee embryo or the compound enzyme of drone pupa quality 0.10-0.18%, mix homogeneously, in the degassed eliminating sargassum smell 10-15min of vacuum-0.07--0.09MPa, stand, continue enzymolysis 10-15min and obtain enzymolysis solution；
Described compound enzyme quality group becomes: flavor protease: acid protease: lipase=2-4:1-3:0.3-0.5；
null2) enzymolysis solution is put 5000-7000r/min traditional vacuum 8-10min in vacuum centrifuge，Separate from top to bottom and obtain free-fat、Emulsion、Hydrolyzed solution and residue，The emulsion obtained is controlled temperature and is 45-55 DEG C，Add the biological demulsifying agent breakdown of emulsion 20-40min of emulsion quality 0.08-0.12%,In 4000-6000r/min traditional vacuum 2 times after breakdown of emulsion，Interval time 1-3h，Centrifugal 10-15min every time，Merge three centrifugal hydrolyzed solutions obtained，Take the Ovum Gallus domesticus album of its quality 0.5-0.7%，Put in blender and stir 10-12min in rotating speed 800-1000r/min,Until Ovum Gallus domesticus album all becomes foam and obtains egg white foams，Egg white foams is added and merges in the hydrolyzed solution obtained，Normal pressure boils 5-8min,It is down to room temperature，In 5000-7000r/min traditional vacuum 8-10min，Collect hydrolyzed solution，Filter through kieselguhr successively、Namely ultrafiltration obtains transparent、Stable queen bee embryo or drone pupa hydrolyzed solution；
3) queen bee embryo or the base liquor of drone pupa hydrolyzed solution quality 1-3 times are taken, it is added thereto to peracetic acid soln, making peroxyacetic acid concentration is 400-480ppm, Homogeneous phase mixing, in power 200-400W, carries out ultrasonic Treatment 5-10min when frequency 30-40KHz, treated base liquor and queen bee embryo or drone pupa hydrolyzed solution Homogeneous phase mixing, control mixed liquor temperature and be 25-35 DEG C, then pass through continuous high voltage pulse process chamber and process, stand, filter and obtain high bioactivity bee product health promoting wine.
2. the preparation method of high bioactivity bee product health promoting wine as claimed in claim 1, it is characterized in that, step 1) described solion is the aqueous solution containing sodium ion 25-30mg/L, zinc ion 17-22mg/L, potassium ion 15-20mg/L, calcium ion 12-14mg/L and magnesium ion 9-11mg/L.
3. the preparation method of high bioactivity bee product health promoting wine as claimed in claim 1, it is characterised in that step 2) described traditional vacuum condition is temperature 6-10 DEG C, vacuum-0.02--0.04MPa.
4. the preparation method of high bioactivity bee product health promoting wine as claimed in claim 1, it is characterised in that step 2) described biological demulsifying agent is that glycolipid class, lipopeptid class, cell wall are in conjunction with the one or more combination in class biological demulsifying agent.
5. the preparation method of high bioactivity bee product health promoting wine as claimed in claim 4, it is characterised in that the quality group of described biological demulsifying agent becomes: glycolipid class: lipopeptid class: cell wall is in conjunction with class=5-7:4-6:2-4.
6. the preparation method of high bioactivity bee product health promoting wine as claimed in claim 5, it is characterised in that described glycolipid class biological demulsifying agent is one or both combinations in rhamnolipid, alkyl polyglucoside；The quality group of described glycolipid class biological demulsifying agent becomes: rhamnolipid: alkyl polyglucoside=3-5:1-2.
7. the preparation method of high bioactivity bee product health promoting wine as claimed in claim 1, it is characterised in that step 3) described base liquor is 40-60 degree Chinese liquor.
8. the preparation method of high bioactivity bee product health promoting wine as claimed in claim 1, it is characterised in that step 3) mass percent concentration of described peracetic acid soln is 10-30%.
9. the preparation method of high bioactivity bee product health promoting wine as claimed in claim 1, it is characterised in that step 3) described high voltage pulse electric field processing condition is electric field intensity 25-30kV/cm, umber of pulse 40-50.
10. the high bioactivity bee product health promoting wine that preparation method as arbitrary in claim 1-9 prepares.
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Application publication date: 20160720 |