CN105777935B - A kind of preparation method of high emulsibility mangrove bark pectin - Google Patents
A kind of preparation method of high emulsibility mangrove bark pectin Download PDFInfo
- Publication number
- CN105777935B CN105777935B CN201610137644.4A CN201610137644A CN105777935B CN 105777935 B CN105777935 B CN 105777935B CN 201610137644 A CN201610137644 A CN 201610137644A CN 105777935 B CN105777935 B CN 105777935B
- Authority
- CN
- China
- Prior art keywords
- pectin
- mangrove bark
- preparation
- mangrove
- high emulsibility
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0045—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Galacturonans, e.g. methyl ester of (alpha-1,4)-linked D-galacturonic acid units, i.e. pectin, or hydrolysis product of methyl ester of alpha-1,4-linked D-galacturonic acid units, i.e. pectinic acid; Derivatives thereof
- C08B37/0048—Processes of extraction from organic materials
Abstract
The invention discloses a kind of preparation method of high emulsibility mangrove bark pectin, comprise the following steps：1) by mangrove bark blanching, be freeze-dried and pulverize and sieve, obtain mangrove bark powder；2) by the step 1) in obtained mangrove bark powder mixed with citric acid solution after obtain mixed liquor, water-bath preheating mixed liquor, the pH value of regulation mixed liquor is 2.0~3.0, carries out ultrasonic extraction, obtains extract solution；3) by the step 2) obtained extract solution carries out separation of solid and liquid, and obtained liquid phase component is precipitated with ethanol mixing, mangrove bark pectin is obtained.The mangrove bark pectin emulsifiability that the method for the invention is obtained is good, recovery rate height (16.70 17.15%)；And the method process time that the present invention is provided is short, pectin thickening property and good stability.
Description
Technical field
The present invention relates to mangrove bark pectin manufacture field, more particularly to a kind of preparation side of high emulsifying activity mangrove bark pectin
Method.
Background technology
Pectin is the mixture of the oligosaccharides that a class is widely present in plant cell and polysaccharide, except with gelling and thickening
Effect is outer, is alternatively arranged as emulsifying agent and maintains emulsion intercalation method, has been widely used in jam, jelly, candy, lactic acid and fruit
In the food such as juice beverage.Pectin is recommended as safe natural additive for foodstuff by FAO/WHO food additives joint committee, and
Do not limit acceptable daily intake.
The domestic research to pectin extraction is more, and achieves certain effect, but more developed country is compared, or exists
Backward in technique, finished product is single, inferior quality the problem of.Because pectin source and extracting mode can be produced to the 26S Proteasome Structure and Function of pectin
Raw considerable influence, researcher constantly looks for new extraction raw material and mode, to obtain the pectin of high-quality, meets all trades and professions
The demand growing to pectin.
The raw material for being presently used for the preparation of commodity pectin is mainly orange peel and pomace, in addition, also having numerous studies from fire
Pectin is extracted in the accessory substances such as Long Guo, beet and granatum, and it is few about the research that pectin is extracted from mangrove bark.Mango is
One of important tropical fruit (tree), China is second largest mango producing country, is only second to India.Because the harvest time of mango is short and fresh
Fruit is easily rotted, therefore in addition to eating raw, mango is also widely processed into mango pulp, mango juice, dried mango, mango powder etc.
Deep processed product.However, often producing the accessory substances such as substantial amounts of mangrove bark, core in mango process, deal with improperly and not only make
Into the serious wasting of resources, and there may be environmental pollution.
The content of the invention
In view of this, it is an object of the invention to by raw material of mango processing byproduct mangrove bark, there is provided a kind of Efficient Ring
Guarantor, method simple and easy to apply, prepare the mangrove bark pectin of high emulsibility.
In order to realize foregoing invention purpose, the present invention provides following technical scheme：
The invention provides a kind of preparation method of high emulsibility mangrove bark pectin, comprise the following steps：
1) by mangrove bark blanching, be freeze-dried and pulverize and sieve, obtain mangrove bark powder；
2) by the step 1) in obtained mangrove bark powder mixed with citric acid solution after obtain mixed liquor, water-bath preheating is mixed
Liquid is closed, the pH value of regulation mixed liquor is 2.0~3.0, carries out ultrasonic extraction, obtains extract solution；
3) by the step 2) obtained extract solution carries out separation of solid and liquid, the liquid phase component that the separation of solid and liquid is obtained with
Ethanol mixing is precipitated, and obtains mangrove bark pectin.
It is preferred that, step 1) described in blanching be steam blanching, time of the blanching is 1~3min.
It is preferred that, step 1) described in sieving be 20~60 mesh sieves.
It is preferred that, it is further comprising the steps of：By step 4) in obtain pectin precipitation carry out vacuum freeze drying, obtain awns
Pericarp pectin.
It is preferred that, step 2) described in citric acid solution concentration be 0.1~0.3mol/L, the citric acid solution with
The mass ratio of mangrove bark powder is (30~50): 1.
It is preferred that, step 2) described in the temperature of water-bath preheating be 60~80 DEG C, time of water-bath preheating for 2~
8min。
It is preferred that, step 2) described in the power of ultrasonic extraction be 400~1000W, the temperature of the ultrasonic extraction is
60~80 DEG C, the time of the ultrasonic extraction is 20~40min.
It is preferred that, separation of solid and liquid described in step 3 by the way of centrifugation, the rotating speed of the centrifugation for 7000~
9000rpm, the time is 5~15min.
It is preferred that, step 3) described in the volume ratio of liquid phase component and ethanol be 1: (1~3), the volumetric concentration of ethanol is
90~95%.
Beneficial effects of the present invention：It is of the invention effectively to prepare pectin using mango processing byproduct mangrove bark for raw material, both
Pollution on the environment is controlled, the added value of mango secondary industry is improved again；The present invention is had using ultrasonic wave-temperature-
The method that machine acid is combined, obtained pectin emulsifiability is good, recovery rate height (16.70-17.15%)；And the present invention is provided
The method process time is short, pectin viscosity height, thickening property and good stability；The mango aqueous pectin solution and 20- of mass concentration 1%
After the mixing of 40% soybean oil, the mango pectin emulsion after homogenization, particle diameter ＜ 15um place emulsion stability ＞ after 7d
80%, stability of emulsion is significantly higher than business citrus pectin.
Brief description of the drawings
The viscosity for the mangrove bark pectin that Fig. 1 obtains for the embodiment of the present invention and the viscosity of citrus pectin are contrasted；
Fig. 2 is the grading curve figure of emulsion prepared by the mangrove bark pectin obtained with the embodiment of the present invention, and wherein A is
Complete the fresh emulsion in homogeneous 5min；B is emulsion after preserving 7 days；
Fig. 3 is the microexamination of the particle diameter of emulsion prepared by the mangrove bark pectin obtained with the embodiment of the present invention, and wherein A is
Citrus pectin emulsion；B-D is mango pectin emulsion；
Fig. 4 is emulsion storage-stable state diagram prepared by the mangrove bark pectin obtained with the embodiment of the present invention,
Wherein A is completes the pectin emulsion state figure in homogeneous 5min, and B is consequence latex solution state diagram in 7 days.
Embodiment
The invention provides a kind of preparation method of high emulsibility mangrove bark pectin, comprise the following steps：
1) by mangrove bark blanching, be freeze-dried and pulverize and sieve, obtain mangrove bark powder；
2) by the step 1) in obtained mangrove bark powder mixed with citric acid solution after obtain mixed liquor, water-bath preheating is mixed
Liquid is closed, the pH value of regulation mixed liquor is 2.0~3.0, carries out ultrasonic extraction, obtains extract solution；
3) by the step 2) obtained extract solution carries out separation of solid and liquid, the liquid phase component that the separation of solid and liquid is obtained with
Ethanol mixing is precipitated, and obtains mangrove bark pectin.
The present invention prepares pectin by raw material of mangrove bark, and mangrove bark is leftover bits and pieces discarded in mango product processing.
In the present invention, the mangrove bark is preferably the fresh mango skin that mango was processed in 1~2 day.The present invention carries out mangrove bark
Blanching, is cleaned preferably before blanching using clear water.In the present invention, the blanching is preferably steam blanching；To described
The time that mangrove bark carries out steam blanching is preferably 1~3min, more preferably 2min.In the present invention, described steam
During blanching, the high temperature of steam can be passivated the endogenous enzymes in mangrove bark, so as to prevent the awns in follow-up processing procedure
Pericarp occurs enzymatic reaction and influences pectin quality.
The present invention is freeze-dried after described mangrove bark carries out blanching.The present invention is preferably in the freeze-drying
Before, precooling is carried out to the mangrove bark after the blanching；In the present invention, the precooling is preferred to use liquid nitrogen progress, the precooling
Time be preferably 2~10min, more preferably 6min.
The vacuum and temperature of heretofore described freeze-drying do not have particular/special requirement, are freeze-dried using this area conventional
Vacuum and temperature；In the present invention, the time of the freeze-drying is preferably 36~60h, more preferably 40
~55h, most preferably 48h.
The present invention crushes the mangrove bark after the freeze-drying after mangrove bark freeze-drying.The present invention is to the powder
Broken method does not have special restriction, using the technical scheme of crushing well known to those skilled in the art；This specific hair
Micromill commonly used in the art can be used to crush the mangrove bark after the freeze-drying for bright embodiment.
After the crushing, the present invention sieves the mangrove bark of the crushing, obtains mangrove bark powder.In the present invention, it is described
Sieving was preferably 20~60 mesh sieves, was more preferably 40~50 mesh sieves.
The currently preferred mangrove bark powder by after sieving is vacuum-packed, and described vacuum packaging is normal using this area
Vacuum-packing method, without other particular/special requirements；It is currently preferred by vacuum packet
Mangrove bark powder progress refrigeration after dress is standby, and the temperature of the refrigeration is preferably 1~5 DEG C, more preferably 4
℃.Obtain after mangrove bark powder, the mangrove bark powder and citric acid solution are mixed to get after mixed liquor by the present invention, and water-filling is entered to it
Bath preheating.In the present invention, the concentration of the citric acid solution is preferably 0.1~0.3mol/L, more preferably 0.2mol/
L；The mass ratio of described citric acid solution and mangrove bark powder is preferably (30~50): 1, more preferably 40: 1, the present invention
It is preferred that mangrove bark powder is well mixed with citric acid solution after, be sealed in Polythene Bag, will be equipped with mangrove bark powder and lemon
The Polythene Bag of acid solution is put into progress water-bath preheating in water-bath, and the temperature of the water-bath preheating is preferably 60~80 DEG C,
More preferably 70 DEG C, the time of the water-bath preheating is preferably 2~8min, more preferably 5min.
The present invention is after water-bath preheating mixed liquor, and the pH value for adjusting the preextraction liquid is 2.0~3.0, carries out ultrasound and carries
Take, obtain extract solution.The present invention is preferably adjusted the pH value of the preextraction liquid to 2.5.PH value regulation is used in the present invention
Solution be citric acid and sodium citrate, adjust mixed liquor pH value after, the present invention to it is described regulation pH value after mixed liquor carry out
Ultrasonic extraction, obtains extract solution.The present invention does not have special limitation to the instrument that the ultrasonic extraction is used, using this area skill
Ultrasonic Cell Disruptor known to art personnel.In the present invention, the power of the ultrasonic extraction is preferably 400~1000W, more
Preferably 500~900W, most preferably 600~800W；The temperature of the ultrasonic extraction is preferably 60~80 DEG C, more preferably
For 70~80 DEG C, most preferably 80 DEG C；The time of the ultrasonic extraction is preferably 20~40min, more preferably 25~
35min, most preferably 30min.The present invention fully extracts the pectin in mangrove bark powder by the ultrasonic extraction.
Extract solution is carried out separation of solid and liquid, obtained liquid phase component is mixed with ethanol by the present invention after extract solution is obtained
Precipitated, obtain mangrove bark pectin.The present invention does not have special limitation to the method for the separation of solid and liquid, using this area skill
The technical scheme of separation of solid and liquid known to art personnel；In the present invention, described separation of solid and liquid is preferably centrifugation；It is described from
The rotating speed of the heart is preferably 7000~9000rpm, more preferably 8000rpm；The time of the centrifugation is preferably 5~
15min, more preferably 10min.
Obtained liquid phase component is precipitated, obtained after separation of solid and liquid is carried out to extract solution by the present invention with ethanol mixing
To mangrove bark pectin.In the present invention, the volume ratio of the liquid phase component and ethanol is preferably 1: (1~3), more preferably
1∶2；The volumetric concentration of the ethanol is preferably 90~95%, more preferably 95%.In the present invention, it is described to be precipitated
Time be preferably 10~14h, more preferably 12h, prolonged ethanol precipitation can be such that pectin is filled in the environment of ethanol
Analyze.
After precipitation terminates, currently preferred use volumetric concentration is washed repeatedly for 95% ethanol to obtained precipitation
Wash, obtain mangrove bark pectin.In the present invention, the number of times of the washing is preferably 2~5 times, more preferably 3~4 times.This
Invention carries out ethanol washing to obtained precipitation, can remove pectin
The pigment of precipitation surface and free neutral sugar；It is currently preferred by the precipitation after the precipitation for obtaining pectin
It is freeze-dried, obtains mangrove bark pectin.The present invention, without particular/special requirement, is used to the condition being freeze-dried that precipitates
The condition of the conventional freeze-drying in this area；In the present invention, the temperature of the freeze-drying is preferably -10 DEG C~-50
DEG C, more preferably -20~-40 DEG C, be most preferably -25~-35 DEG C；The vacuum of the freeze-drying is preferably 1.3~
13Pa, more preferably 2~10Pa.
The preparation method of the high emulsibility mangrove bark pectin provided with reference to embodiment the present invention is carried out specifically
It is bright, but they can not be interpreted as limiting the scope of the present invention.
Embodiment 1
Fresh mango skin is cleaned with clear water, and steam blanching 2min is to be passivated endogenous enzymes, and Liquid nitrogen precooler 6min is simultaneously freezed
Crushed after drying 48h, 40 mesh sieves are crossed after crushing and obtain mangrove bark powder, be vacuum-packed after mangrove bark powder that to be put in 4 DEG C of refrigerations standby
With；Mixed with mangrove bark powder and 0.2mol/L citric acid solutions, citric acid solution is 40: 1 with the mass ratio of mangrove bark powder；By lemon
Lemon acid sodium solution is sealed in Polythene Bag after being mixed with mangrove bark powder, and Polythene Bag is placed in into water-bath in 70 DEG C of water-baths preheats
5min, obtains preextraction liquid；Obtained preextraction liquid pH is adjusted to 2.5 using citric acid and sodium citrate, power is put into
600W Ultrasound Instrument ultrasonic extraction 30min under the conditions of 70 DEG C, fully extracts pectin, obtains extract solution；By obtained extract solution
10min is centrifuged under the conditions of 8000rpm, 95% (v/v) second of 2 times of liquid phase component volumes is added into the liquid phase component being collected into
Alcohol precipitates 12h, so that pectin is fully separated out；Gained precipitation 95% (v/v) ethanol cyclic washing 3 times is heavy to remove pectin
Pigment and free neutral sugar in shallow lake；Obtained precipitation is obtained into mangrove bark pectin after vacuum freeze drying.
The yield for calculating mangrove bark pectin obtained above is 16.70%；
The present invention determines the viscosity of mangrove bark pectin using viscosimeter, as a result as shown in figure 1, Fig. 1 is pectin viscosity with cutting
Shear force change curve, as seen from Figure 1, pectin solution viscosity is big, thickening property is good (accompanying drawing 1- mango pectin 1)；Present invention inspection
Emulsion particle diameter distribution prepared by the pectin measured, as a result as shown in Figures 2 and 3, Fig. 2 is pectin emulsion particle diameter distribution curve,
Fig. 3 is the micro- sem observation figure of pectin emulsion particle diameter, the pectin emulsion that the present embodiment is prepared it can be seen from Fig. 2 and Fig. 3
15 μm of product cut size ＜ (accompanying drawing 2- mango pectin 1, accompanying drawing 3-B).
Mangrove bark pectin obtained above is configured to the mangrove bark aqueous pectin solution of mass concentration 1% by the present invention, with 1%
The business citrus pectin aqueous solution be control, described business citrus pectin is common commercially available citrus pectin, by mango follicarpium
After glue solution and the control citrus pectin aqueous solution are mixed with 40% soybean oil,
Mango pectin emulsion and citrus pectin emulsion are obtained under 10000rpm using homogenizer after homogenization 1min, it is micro-
The particle diameter of sem observation pectin emulsion, observes its stability, pectin emulsion state as shown in figure 4, wherein A is even to complete after placing 7 days
Pectin emulsion state figure in matter 5min, B is consequence latex solution state diagram in 7 days, it can be seen that, mango follicarpium of the present invention
Emulsion stability is good (accompanying drawing 4- mango pectin 1) after glue placement 7d, and stability of emulsion is significantly higher than business citrus pectin.
Embodiment 2
Fresh mango skin is cleaned with clear water, and steam blanching 2min is to be passivated endogenous enzymes, and Liquid nitrogen precooler 8min is simultaneously freezed
Crushed after drying 52h, 40 mesh sieves are crossed after crushing and obtain mangrove bark powder, be vacuum-packed after mangrove bark powder that to be put in 4 DEG C of refrigerations standby
With；Mixed with mangrove bark powder and 0.2mol/L citric acid solutions, citric acid solution is 30: 1 with the mass ratio of mangrove bark powder；By lemon
Lemon acid sodium solution is sealed in Polythene Bag after being mixed with mangrove bark powder, is placed in preheating 6min in 60 DEG C of water-baths and is obtained preextraction
Liquid；Obtained preextraction liquid pH is adjusted to 2.3 using citric acid and sodium citrate, power 800W Ultrasound Instrument is put into 60 DEG C
Under the conditions of handle 20min, fully extract pectin and obtain extract solution, that collects that liquid phase component obtains after 7000rpm centrifugations 8min carries
Liquid is taken, 2 times of ethanol precipitation 14h of volume 95% are added into the liquid phase component being collected into so that pectin is fully separated out, gained is precipitated
With 95% ethanol cyclic washing 5 times, to remove the pigment and free neutral sugar in pectin precipitation；By obtained precipitation through true
Mangrove bark pectin is obtained after vacuum freecing-dry.
The yield for calculating mangrove bark pectin obtained above is 16.90%；
The present invention determines the viscosity of mangrove bark pectin using viscosimeter, as a result as shown in figure 1, Fig. 1 is pectin viscosity with cutting
Shear force change curve, as seen from Figure 1, pectin solution viscosity is big, thickening property is good (accompanying drawing 1- mango pectin 2)；
The particle diameter distribution for the pectin emulsion that present invention detection is obtained, as a result as shown in Figures 2 and 3, Fig. 2 are pectin particle diameter point
Cloth curve, Fig. 3 is the micro- sem observation figure of pectin emulsion particle diameter, and particle diameter ＜ 15um (accompanying drawing 2- mango is can be seen that by Fig. 2 and Fig. 3
Pectin 2, accompanying drawing 3-C)；
Mangrove bark pectin obtained above is configured to the mangrove bark aqueous pectin solution of mass concentration 1% by the present invention, with 1%
The business citrus pectin aqueous solution be control, described business citrus pectin is common commercially available citrus pectin, by mango follicarpium
After glue solution and the control citrus pectin aqueous solution are mixed with 40% soybean oil, using homogenizer homogenization under 10000rpm
Mango pectin emulsion and citrus pectin emulsion are obtained after 1min, the particle diameter of micro- sem observation pectin emulsion observes its steady after placing 7 days
Qualitative, pectin emulsion state is as shown in figure 4, wherein A is completes the pectin emulsion state figure in homogeneous 5min, and B is 7 days consequence latex
Liquid status figure, it can be seen that, mangrove bark pectin of the present invention places good (the accompanying drawing 4- awns of emulsion stability after 7d
Fruit pectin 2), stability of emulsion is significantly higher than business citrus pectin.
Embodiment 3
Fresh mango skin is cleaned with clear water, and steam blanching 2min is to be passivated endogenous enzymes, and Liquid nitrogen precooler 4min is simultaneously freezed
Crushed after drying 40h, 40 mesh sieves are crossed after crushing and obtain mangrove bark powder, be vacuum-packed after mangrove bark powder that to be put in 4 DEG C of refrigerations standby
With；Mixed with mangrove bark powder and 0.25mol/L citric acid solutions, citric acid solution is 50: 1 with the mass ratio of mangrove bark powder；Will
Citric acid solution is sealed in Polythene Bag after being mixed with mangrove bark powder, is placed in preextraction 6min in 80 DEG C of water-baths and is obtained pre- carry
Take liquid；Obtained preextraction liquid pH is adjusted to 2.8 using citric acid and sodium citrate, power 500W Ultrasound Instrument is put into 80
40min is handled under the conditions of DEG C, fully extracts after pectin obtains extract solution, 9000rpm centrifugations 12min and collects what liquid phase component was obtained
Extract solution, adds 2 times of ethanol precipitation 10h of volume 95% so that pectin is fully separated out, gained sinks into the liquid phase component being collected into
Form sediment with 95% ethanol cyclic washing 4 times, with the neutral sugar for removing the pigment in pectin precipitation and dissociating；By obtained precipitation warp
Mangrove bark pectin is obtained after vacuum freeze drying.
The yield for calculating mangrove bark pectin obtained above is 16.90%；
The present invention determines the viscosity of mangrove bark pectin using viscosimeter, as a result as shown in figure 1, Fig. 1 is pectin viscosity with cutting
Shear force change curve, as seen from Figure 1, pectin solution viscosity is big, thickening property is good (accompanying drawing 1- mango pectin 3)；
The particle diameter distribution for the pectin emulsion that present invention detection is obtained, as a result as shown in Figures 2 and 3, Fig. 2 are pectin emulsion grain
Footpath distribution curve, particle diameter ＜ 15um (the accompanying drawing 2- mango pectin 3, accompanying drawing 3-D) it can be seen from Fig. 2 and Fig. 3；
Mangrove bark pectin obtained above is configured to the mangrove bark aqueous pectin solution of mass concentration 1% by the present invention, with 1%
The business citrus pectin aqueous solution be control, described business citrus pectin is common commercially available citrus pectin, by mango follicarpium
After glue solution and the control citrus pectin aqueous solution are mixed with 40% soybean oil, using homogenizer homogenization under 10000rpm
Mango pectin emulsion and citrus pectin emulsion are obtained after 1min, the particle diameter of micro- sem observation pectin emulsion observes its steady after placing 7 days
Qualitative, pectin emulsion state is as shown in figure 4, wherein A is completes the pectin emulsion state figure in homogeneous 5min, and B is 7 days consequence latex
Liquid status figure, it can be seen that, mangrove bark pectin of the present invention places good (the accompanying drawing 4- mango pectin of emulsion stability after 7d
3), stability of emulsion is significantly higher than business citrus pectin.
As seen from the above embodiment, the preparation method for the high emulsibility mangrove bark pectin that the present invention is prepared is prepared
Mangrove bark pectin yield it is high, pectin solution viscosity is big, thickening property is good, and emulsion stability is good (particle diameter ＜ 15um), emulsion-stabilizing
Property is significantly higher than business citrus pectin.
The above is only the preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, under the premise without departing from the principles of the invention, some improvements and modifications can also be made, these improvements and modifications also should
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of preparation method of high emulsibility mangrove bark pectin, comprises the following steps：
1) by mangrove bark blanching, be freeze-dried and pulverize and sieve, obtain mangrove bark powder；
2) by the step 1) in obtained mangrove bark powder mixed with citric acid solution after obtain mixed liquor, the pre- hot mixing of water-bath
Liquid, the pH value of regulation mixed liquor is 2.0~3.0, carries out ultrasonic extraction, obtains extract solution；
3) by the step 2) obtained extract solution carries out separation of solid and liquid, the liquid phase component and ethanol that the separation of solid and liquid is obtained
Mixing is precipitated, and obtains mangrove bark pectin.
2. a kind of preparation method of high emulsibility mangrove bark pectin according to claim 1, it is characterised in that step 1) in
Described blanching is steam blanching, and the time of the blanching is 1~3min.
3. a kind of preparation method of high emulsibility mangrove bark pectin according to claim 1, it is characterised in that step 1) in
Described sieving was 20~60 mesh sieves.
4. a kind of preparation method of high emulsibility mangrove bark pectin according to claim 1, it is characterised in that step 2) in
The concentration of described citric acid solution is 0.1~0.3mol/L, the mass ratio of the citric acid solution and mangrove bark powder for (30~
50):1。
5. a kind of preparation method of high emulsibility mangrove bark pectin according to claim 1, it is characterised in that step 2) in
The temperature of described water-bath preheating is 60~80 DEG C, and the time of the preheating is 2~8min.
6. a kind of preparation method of high emulsibility mangrove bark pectin according to claim 1, it is characterised in that step 2) in
The power of described ultrasonic extraction is 400~1000W, and the temperature of the ultrasonic extraction is 60~80 DEG C, the ultrasonic extraction
Time is 20~40min.
7. a kind of preparation method of high emulsibility mangrove bark pectin according to claim 1, it is characterised in that step 3) in
Described separation of solid and liquid is centrifugation, and the rotating speed of the centrifugation is 7000~9000rpm, and the time of the centrifugation is 5~15min.
8. a kind of preparation method of high emulsibility mangrove bark pectin according to claim 1, it is characterised in that step 3) in
The volume ratio of the liquid phase component and ethanol is 1:(1~3).
9. a kind of preparation method of high emulsibility mangrove bark pectin according to claim 8, it is characterised in that the ethanol
Volumetric concentration be 90~95%.
10. a kind of preparation method of high emulsibility mangrove bark pectin according to claim 1~9 any one, its feature
Be, the step 3) precipitated after also include：By step 3) in obtained precipitation carry out vacuum freeze drying, obtain mango
Follicarpium glue.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610137644.4A CN105777935B (en) | 2016-03-11 | 2016-03-11 | A kind of preparation method of high emulsibility mangrove bark pectin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610137644.4A CN105777935B (en) | 2016-03-11 | 2016-03-11 | A kind of preparation method of high emulsibility mangrove bark pectin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105777935A CN105777935A (en) | 2016-07-20 |
| CN105777935B true CN105777935B (en) | 2017-07-14 |
Family
ID=56392417
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610137644.4A Active CN105777935B (en) | 2016-03-11 | 2016-03-11 | A kind of preparation method of high emulsibility mangrove bark pectin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN105777935B (en) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106866839A (en) * | 2017-03-13 | 2017-06-20 | 浙江大学 | The method that ultrasonic hot acid method extracts LM in Hu shaddock ped |
| CN110699405B (en) * | 2019-11-20 | 2023-03-24 | 江南大学 | Method for preparing mango peel pectin oligosaccharides with antibacterial activity by using immobilized polygalacturonase |
| CN111349183B (en) * | 2020-04-22 | 2022-04-01 | 黑龙江八一农垦大学 | Pectin and its extraction method and preparation method |
| CN112056544B (en) * | 2020-09-21 | 2022-07-29 | 华中农业大学 | Preparation method of pectin emulsion gel capable of stably loading fat-soluble active ingredients |
| CN114752001A (en) * | 2022-04-11 | 2022-07-15 | 华中农业大学 | High-emulsibility persimmon pectin and preparation method and application thereof |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TR201909135T4 (en) * | 2007-08-29 | 2019-07-22 | Kmc Kartoffelmelcentralen Amba | Fiber-containing pectin product preparation method and related pectin products. |
| CN101942036A (en) * | 2009-07-08 | 2011-01-12 | 李浩贤 | Method for extracting pectin from pineapple peel assisted by ultrasonic wave |
| CN104086667B (en) * | 2014-06-11 | 2017-03-08 | 安徽农业大学 | A kind of method preparing citruss skin slag pectin using ultrasonic assistant extraction |
| CN104710544A (en) * | 2015-03-31 | 2015-06-17 | 川渝中烟工业有限责任公司 | Method for extracting pectin from tobacco waste by using ultrasonic and organic acid |
| CN105237654A (en) * | 2015-11-16 | 2016-01-13 | 陈兴春 | Method for extracting pectin from orange peel |
-
2016
- 2016-03-11 CN CN201610137644.4A patent/CN105777935B/en active Active
Also Published As
| Publication number | Publication date |
|---|---|
| CN105777935A (en) | 2016-07-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105777935B (en) | A kind of preparation method of high emulsibility mangrove bark pectin | |
| CN102835706A (en) | Method for enhancing aroma of mango pulp | |
| CN108477455B (en) | Preparation method of whole citrus fruit superfine powder and whole citrus fruit superfine powder | |
| CN107129859A (en) | A kind of processing method for improving the fresh oil cut rate of squeezing out of olive | |
| CN104774228A (en) | Method for preparing persimmon tannin from persimmon | |
| CN104788511A (en) | Method for preparing persimmon tannins with high micromolecular tannin content from persimmons | |
| CN104432078B (en) | A kind of black oral liquid of Chinese wolfberry and its preparation method | |
| CN104187990A (en) | Peach source beverage and preparation method thereof | |
| CN105558506A (en) | Marinating material for stewed duck with bean sauce | |
| CN111096447A (en) | Tremella pulp, preparation method and application thereof, thickener and preparation method thereof | |
| CN102871178A (en) | Malus micromalus makino compound juice and production method thereof | |
| CN106937961B (en) | Water-soluble ganoderma lucidum powder and preparation method thereof | |
| CN107573438A (en) | A kind of method that polysaccharide is extracted in the pericarp from passion fruit | |
| CN107772185A (en) | The preparation method of grape Dragonfruit Juice | |
| CN107095214A (en) | A kind of Rosa roxburghii Siberian solomonseal rhizome health-care powder and preparation method thereof | |
| CN106190609A (en) | The preparation method of high active ingredient tea cake powder detergent and tea cake powder detergent | |
| CN106262105A (en) | A kind of processing method of vacuum lyophilization Fructus Cucurbitae moschatae powder | |
| CN106579144A (en) | Preparation method of purple sweet potato powder | |
| CN104904969A (en) | Maca coffee | |
| CN105901583A (en) | Processing method for coproducing sweet potato juice, starch and total-sweet potato starch by using sweet potato | |
| CN105661215A (en) | Whole kiwi fruit juice and processing method thereof | |
| CN104177483A (en) | Method for synchronously and efficiently preparing chuanminshen violaceum protein and polysaccharide | |
| CN103462034A (en) | Winy cereal fruit-vegetable powder and its preparation method | |
| CN106666634A (en) | Preparation method of natural compound sweetener | |
| CN106472941A (en) | A kind of pineapple sarcocarp powder solid beverage technological method for processing |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |