CN104710544A - Method for extracting pectin from tobacco waste by using ultrasonic and organic acid - Google Patents
Method for extracting pectin from tobacco waste by using ultrasonic and organic acid Download PDFInfo
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- CN104710544A CN104710544A CN201510149735.5A CN201510149735A CN104710544A CN 104710544 A CN104710544 A CN 104710544A CN 201510149735 A CN201510149735 A CN 201510149735A CN 104710544 A CN104710544 A CN 104710544A
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- pectin
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Abstract
The invention discloses a method for extracting pectin from tobacco waste by using ultrasonic and organic acid, which comprises the following steps: drying the tobacco waste, pulverizing into powder, and screening; preparing an organic acid solution from water and citric acid crystal, regulating the pH value of the solution to 0.5-2.5, and adding the tobacco waste powder; treating in an ultrasonic instrument with the ultrasonic power of 500-1000W for 30-60 minutes to obtain a turbid liquid, carrying out vacuum filtration, removing the filter residue to obtain a pectin crude extracting solution, adding activated carbon for decolorization, and cooling for later use; and washing with anhydrous ethanol to obtain white pectin, carrying out vacuum drying on the white pectin to obtain offwhite pectin, and pulverizing to obtain the product. The method has the advantages of higher pectin yield and controllable extraction process, and is milder and more effective than the traditional pectin extraction process by using inorganic acid.
Description
Technical field
The present invention relates to tobacco field waste recovery and reuse technology field, be specifically related to a kind of chemical technology method utilizing ultrasonic wave-organic acidity extraction tobacco waste.
Background technology
Tobacco waste refers to except the tobacco leaf with industrial usability, other positions of tobacco plant, as tobacco rod, cigarette root, fireworks, cigarette wooden fork, axillalry bud, beat top and pin leaf that leaf removes as tobacco waste, be usually discarded in field in a large number or store and go rotten.China is tobacco planting big country, just has such a tobacco waste just to have hundreds of thousands of ton to be wasted every year, causes the loss of resource and the pollution of environment.The comprehensive utilization of tobacco is proposed by state monopoly office very early, and two Conferences in 2015 especially environmentally friendly type manufacturing industry is had higher requirement, and the recycling of tobacco waste needs to introduce more scientific and technical content.
Pectin is a kind of natural polymers, and its basic structure is D-galactopyranosyluronic, usually exists with part esterification state, is extensively present in the cell walls of the root of higher plant (comprising tobacco), stem, leaf, fruit etc.Now, the raw material being used for extracting pectin mainly contains orange peel, sunflower plate, Pericarpium Mali pumilae, Watermelon rind, Pericarpium Kaki etc., and about paper, the report of extracting pectin from tobacco few, but show in tobacco by research, the pectin content particularly in offal enriches.Tradition is used in the extractive technique mainly mineral acid extraction method of pectin in tobacco, and extraction agent is nitric acid, hydrochloric acid etc. mainly, and because it is acid, corrodibility is comparatively strong, so higher to the cost requirement of equipment, environmental pollution is also heavier.
Summary of the invention
Instant invention overcomes the deficiencies in the prior art, a kind of method utilizing ultrasonic wave and organic acid to extract pectin from tobacco waste is provided, to expect the extract yield improving pectin, reduce process costs.
Consider the problems referred to above of prior art, according to an aspect disclosed by the invention, the present invention by the following technical solutions:
Utilize ultrasonic wave and organic acid from tobacco waste, extract a method for pectin, it comprises the following steps:
Step one, tobacco waste to be dried, then be ground into powder and sieve;
Step 2, use water and Citric acid crystal prepare organic acid soln, and regulator solution pH is 0.5 to 2.5, then puts into tobacco waste powder;
Step 3, to put into ultrasonic power be process 30 to 60min under the ultrasonic apparatus of 500 to 1000W, obtains suction filtration after turbid solution, obtain pectin crude extract, then add activated carbon decolorizing, then cool for subsequent use after removing filter residue;
Step 4, obtain white pectin with absolute ethanol washing, this pectin vacuum-drying is obtained canescence pectin, is pulverized, obtain product.
In order to realize the present invention better, further technical scheme is:
According to one embodiment of the invention, dry in described step one and adopt electric heating constant-temperature blowing drying box dry under 50 DEG C of constant temperature.
According to another embodiment of the invention, the size that in described step one, tobacco waste powder sieves is 60 to 100 mesh sieves.
According to another embodiment of the invention, described water is ultrapure water.
According to another embodiment of the invention, putting in described step 2 after tobacco waste powder and stir with glass stick, having not by the agglomerated powders dissolved when ultrasonic apparatus process for avoiding.
According to another embodiment of the invention, in described step 3, gac decolours 30min under 60 DEG C of conditions.
According to another embodiment of the invention, decolour with the charcoal absorption that massfraction is 3-4% after extracting liquid filtering in described step 3.
The present invention can also be:
According to another embodiment of the invention, in described step 4, dehydrated alcohol adition process is slow, and slowly stirs simultaneously.
Compared with prior art, one of beneficial effect of the present invention is:
A kind of method utilizing ultrasonic wave and organic acid to extract pectin from tobacco waste of the present invention, Appropriate application tobacco waste, be namely the recycling to waste resource, be again effective control of environmental pollution; Found through experiments, improve the productive rate of pectin, make it reach the yield of 15%, improve 4% than ethanol extraction method, and extraction process is easy to control, extract pectin than traditional mineral acid more gentle, effective.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
In the cell walls that pectin is often present in plant tobacco and middle lamella, the technology making biomass broken wall can be utilized, in conjunction with ultrasonic wave-organic acid system, can extraction efficiency be improved, extraction process can be made again simple and easy, gentle, change the deficiency in traditional method.The tobacco waste of indication in the present invention, comprises offal, tobacco rod, cigarette wooden fork, offal, tobacco stem and tobacco leaf etc.Below in conjunction with embodiment, the present invention is described further, but be not limited to embodiment.
Embodiment 1
Utilize ultrasonic wave and organic acid from tobacco waste, extract a method for pectin, it comprises the following steps:
A. tobacco waste is put into electric heating constant-temperature blowing drying box 50 DEG C oven dry, be ground into powder and sieve, make size even, the size that preferred tobacco waste powder sieves is 60 to 100 mesh sieves.
B. in beaker, ultrapure water is loaded, add Citric acid crystal preparation organic acid soln, regulator solution pH is 0.5 to 2.5, then puts into tobacco waste powder, after putting into tobacco waste powder, useable glass rod constantly fully stirs, in order to avoid also have agglomerating powder not dissolved when ultrasonic apparatus process.
C. beaker being put into ultrasonic power is process 30 to 60min under the ultrasonic apparatus of 500 to 1000W, obtain suction filtration after turbid solution, pectin crude extract is obtained after removing filter residue, finally add proper amount of active carbon to decolour under 60 DEG C of conditions 30min, preferably, decolour with the charcoal absorption that massfraction is 3-4% after extracting liquid filtering; Then cool for subsequent use.
D. obtain white pectin with absolute ethanol washing, dehydrated alcohol preferably slowly adds, and slowly stirs, by this pectin vacuum drying oven 70 DEG C carry out drying, finally obtain canescence pectin, pulverized, obtain product.
Embodiment 2
A. the tobacco rod process by discarded for land for growing field crops is clean, after putting into electric heating constant-temperature blowing drying box 50 DEG C oven dry, screening, impurity elimination, is ground into powder and crosses 60 mesh sieves, making size even.
B. by loading 250ml ultrapure water in beaker, slowly adding Citric acid crystal, stirring, is 2.5 with precision test paper regulator solution pH, then puts into the tobacco waste powder handled well, fully stirs with glass stick, make it be uniformly distributed in organic solution.
C. under beaker being put into ultrasonic apparatus, make the liquid level in ultrasonic apparatus exceed beaker liquid level of solution, then regulate ultrasonic apparatus power to 500W, the treatment time continues 30min.Obtain suction filtration after turbid solution, obtain pectin crude extract after removing filter residue, last extraction liquid adds 3% gac adsorption bleaching 30min under 60 DEG C of conditions, cools for subsequent use.
D. finally slowly adding absolute ethanol washing obtains white pectin, is 2%, finally obtains 70 DEG C are carried out being dried to water content in this pectin vacuum drying oven canescence pectin, pulverized.The yield of pectin is 13.4% after testing, and the pectin color and luster of this experiment gained is better.
Embodiment 3
A. the tobacco rod process by discarded for land for growing field crops is clean, after putting into electric heating constant-temperature blowing drying box 50 DEG C oven dry, screening, impurity elimination, is ground into powder and crosses 80 mesh sieves, making size even.
B. by loading 250ml ultrapure water in beaker, slowly adding Citric acid crystal, stirring, is 1.5 with precision test paper regulator solution pH, then puts into the tobacco waste powder handled well, fully stirs with glass stick, make it be uniformly distributed in organic solution.
C. under beaker being put into ultrasonic apparatus, make the liquid level in ultrasonic apparatus exceed beaker liquid level of solution, then regulate ultrasonic apparatus power to 800W, the treatment time continues 40min.Obtain suction filtration after turbid solution, obtain pectin crude extract after removing filter residue, last extraction liquid adds 4% gac adsorption bleaching 30min under 60 DEG C of conditions, cools for subsequent use.
D. finally slowly adding absolute ethanol washing obtains white pectin, is 2%, finally obtains 70 DEG C are carried out being dried to water content in this pectin vacuum drying oven canescence pectin, pulverized.The yield of pectin is 14.2% after testing, and the pectin color and luster of this experiment gained is better.
Embodiment 4
A. the tobacco rod process by discarded for land for growing field crops is clean, after putting into electric heating constant-temperature blowing drying box 50 DEG C oven dry, screening, impurity elimination, is ground into powder and crosses 100 mesh sieves, making size even.
B. by loading 250ml ultrapure water in beaker, slowly adding Citric acid crystal, stirring, is 0.5 with precision test paper regulator solution pH, then puts into the tobacco waste powder handled well, fully stirs with glass stick, make it be uniformly distributed in organic solution.
C. under beaker being put into ultrasonic apparatus, make the liquid level in ultrasonic apparatus exceed beaker liquid level of solution, then regulate ultrasonic apparatus power to 1000W, the treatment time continues 60min.Obtain suction filtration after turbid solution, obtain pectin crude extract after removing filter residue, last extraction liquid adds 4% gac adsorption bleaching 30min under 60 DEG C of conditions, cools for subsequent use.
D. finally slowly adding absolute ethanol washing obtains white pectin, is 2%, finally obtains 70 DEG C are carried out being dried to water content in this pectin vacuum drying oven canescence pectin, pulverized.The yield of pectin is 15.2% after testing, and the pectin color and luster of this experiment gained is better.
In this specification sheets, each embodiment adopts the mode of going forward one by one to describe, and what each embodiment stressed is the difference with other embodiment, identical similar portion cross-reference between each embodiment.
Spoken of in this manual " embodiment ", " another embodiment ", " embodiment ", etc., refer to the specific features, structure or the feature that describe in conjunction with this embodiment and be included at least one embodiment of the application's generality description.Multiple place occurs that statement of the same race is not necessarily refer to same embodiment in the description.Furthermore, when describing specific features, structure or a feature in conjunction with any embodiment, what advocate is also fall within the scope of the invention to realize this feature, structure or feature in conjunction with other embodiments.
Although with reference to multiple explanatory embodiment of the present invention, invention has been described here, but, should be appreciated that, those skilled in the art can design a lot of other amendment and embodiment, these amendments and embodiment will drop within spirit disclosed in the present application and spirit.More particularly, in the scope of and claim open in the application, multiple modification and improvement can be carried out to the building block of subject combination layout and/or layout.Except the modification of carrying out building block and/or layout is with except improvement, to those skilled in the art, other purposes also will be obvious.
Claims (8)
1. utilize ultrasonic wave and organic acid from tobacco waste, extract a method for pectin, it is characterized in that it comprises the following steps:
Step one, tobacco waste to be dried, then be ground into powder and sieve;
Step 2, use water and Citric acid crystal prepare organic acid soln, and regulator solution pH is 0.5 to 2.5, then puts into tobacco waste powder;
Step 3, to put into ultrasonic power be process 30 to 60min under the ultrasonic apparatus of 500 to 1000W, obtains suction filtration after turbid solution, obtain pectin crude extract, then add activated carbon decolorizing, then cool for subsequent use after removing filter residue;
Step 4, obtain white pectin with absolute ethanol washing, this pectin vacuum-drying is obtained canescence pectin, then is pulverized, obtain product.
2. the method utilizing ultrasonic wave and organic acid to extract pectin from tobacco waste according to claim 1, is characterized in that drying employing electric heating constant-temperature blowing drying box in described step one dry under 50 DEG C of constant temperature.
3. the method utilizing ultrasonic wave and organic acid to extract pectin from tobacco waste according to claim 1, is characterized in that the size that in described step one, tobacco waste powder sieves is 60 to 100 mesh sieves.
4. the method utilizing ultrasonic wave and organic acid to extract pectin from tobacco waste according to claim 1, is characterized in that described water is ultrapure water.
5. the method utilizing ultrasonic wave and organic acid to extract pectin from tobacco waste according to claim 1, stirring with glass stick after it is characterized in that putting in described step 2 tobacco waste powder, having not by the agglomerated powders dissolved when ultrasonic apparatus process for avoiding.
6. the method utilizing ultrasonic wave and organic acid to extract pectin from tobacco waste according to claim 1, to is characterized in that in described step 3 that gac decolours 30min under 60 DEG C of conditions.
7. the method utilizing ultrasonic wave and organic acid to extract pectin from tobacco waste according to claim 1, is characterized in that decolouring with the charcoal absorption that massfraction is 3-4% after extracting liquid filtering in described step 3.
8. the method utilizing ultrasonic wave and organic acid to extract pectin from tobacco waste according to claim 1, is characterized in that in described step 4, dehydrated alcohol adition process is slow, and slowly stirs simultaneously.
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| CN201510149735.5A CN104710544A (en) | 2015-03-31 | 2015-03-31 | Method for extracting pectin from tobacco waste by using ultrasonic and organic acid |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105566512A (en) * | 2015-12-14 | 2016-05-11 | 山东省果树研究所 | Extracting method of persimmon fruit pectin |
| CN105777935A (en) * | 2016-03-11 | 2016-07-20 | 华中农业大学 | Preparation method of high-emulsibility mango peel pectin |
| CN115490782A (en) * | 2022-10-12 | 2022-12-20 | 昆明理工大学 | Method for extracting pectin from tobacco stems by using ultrasonic waves and mixed acid |
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2015
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| CN101805416A (en) * | 2010-03-16 | 2010-08-18 | 上海交通大学 | Preparation method of canna edulis ker pectin |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105566512A (en) * | 2015-12-14 | 2016-05-11 | 山东省果树研究所 | Extracting method of persimmon fruit pectin |
| CN105566512B (en) * | 2015-12-14 | 2018-03-30 | 山东省果树研究所 | A kind of extracting method of persimmon fruit pectin |
| CN105777935A (en) * | 2016-03-11 | 2016-07-20 | 华中农业大学 | Preparation method of high-emulsibility mango peel pectin |
| CN115490782A (en) * | 2022-10-12 | 2022-12-20 | 昆明理工大学 | Method for extracting pectin from tobacco stems by using ultrasonic waves and mixed acid |
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