CN105777935A - Preparation method of high-emulsibility mango peel pectin - Google Patents
Preparation method of high-emulsibility mango peel pectin Download PDFInfo
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- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0006—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid
- C08B37/0045—Homoglycans, i.e. polysaccharides having a main chain consisting of one single sugar, e.g. colominic acid alpha-D-Galacturonans, e.g. methyl ester of (alpha-1,4)-linked D-galacturonic acid units, i.e. pectin, or hydrolysis product of methyl ester of alpha-1,4-linked D-galacturonic acid units, i.e. pectinic acid; Derivatives thereof
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Abstract
The invention discloses a preparation method of high-emulsibility mango peel pectin. The preparation method comprises the following steps: (1) performing hot ironing, freeze drying and smashing screening on mango peels to obtain mango peel powder; (2) mixing the mango peel powder obtained in the step (1) with a citric acid solution to obtain a mixed solution, preheating the mixed solution in a water bath, adjusting the pH value of the mixed solution to 2.0 to 3.0, and performing ultrasonic extraction to obtain an extracting solution; (3) performing solid-liquid separation on the extracting solution obtained in the step (2), mixing an obtained liquid phase component with ethyl alcohol for precipitation, to obtain the mango peel pectin. The mango peel pectin prepared by the preparation method is high in emulsification performance and extracting rate (16.70 to 17.15 percent); furthermore, the method provided by the invention is short in process time, and high in pectin thickening property and stability.
Description
Technical field
The present invention relates to mangrove bark pectin manufacture field, particularly relate to a kind of high emulsifying activity mangrove bark pectin
Preparation method.
Background technology
Pectin is the oligosaccharides that is widely present in plant cell of a class and the mixture of polysaccharide, except having glue
Coagulate and outside thickening power, be alternatively arranged as emulsifying agent and maintain emulsion intercalation method, be widely used in fruit
In the food such as sauce, jelly, candy, lactic acid and fruit drink.Committee combined by FAO/WHO food additives
Pectin can be recommended as safe natural additive for foodstuff by member, and does not limit acceptable daily intake.
The domestic research to pectin extraction is more, and achieves certain effect, but more developed country is compared,
Still exist backward in technique, finished product is single, the problem of inferior quality.Due to pectin source and extracting mode
The 26S Proteasome Structure and Function of pectin can be produced considerable influence, and researcher constantly looks for new extraction raw material and mode,
To obtaining the pectin of high-quality, meet the demand that all trades and professions are growing to pectin.
It is presently used for raw material prepared by commodity pectin and is mainly orange peel and pomace, additionally, also have big
Quantity research extracts pectin from the accessory substances such as dragon fruit, beet and granatum, and relevant carries from mangrove bark
The research taking pectin is few.Mango is one of important tropical fruit (tree), and China is second largest mango producing country,
It is only second to India.Owing to the harvest time of mango is short, and fresh fruit easily rots, therefore in addition to eating raw,
Mango is also processed into the deep processed products such as mango pulp, mango juice, dried mango, mango powder widely.So
And, mango process often produces the accessory substances such as substantial amounts of mangrove bark, core, deals with improperly and not only make
Become the serious wasting of resources, and environmental pollution may be produced.
Summary of the invention
In view of this, it is an object of the invention to mango processing byproduct mangrove bark as raw material, it is provided that one
Plant high-efficiency environment friendly, simple method, prepare the mangrove bark pectin of high emulsibility.
In order to realize foregoing invention purpose, the present invention provides techniques below scheme:
The invention provides the preparation method of a kind of high emulsibility mangrove bark pectin, comprise the following steps:
1) by mangrove bark blanching, freeze-drying with pulverize and sieve, mangrove bark powder is obtained；
2) by described step 1) in the mangrove bark powder that obtains mix with citric acid solution after obtain mixed liquor,
Water-bath preheating mixed liquor, the pH value of regulation mixed liquor is 2.0～3.0, carries out ultrasonic extraction, is extracted
Liquid；
3) by described step 2) extract that obtains carries out separation of solid and liquid, described separation of solid and liquid obtained
Liquid phase component mixes with ethanol and precipitates, and obtains mangrove bark pectin.
Preferably, step 1) described in blanching be steam blanching, the time of described blanching is 1～3min.
Preferably, step 1) described in sieve for cross 20～60 mesh sieves.
Preferably, further comprising the steps of: by step 4) in obtain pectin precipitation carry out vacuum refrigeration do
Dry, obtain mangrove bark pectin.
Preferably, step 2) described in the concentration of citric acid solution be 0.1～0.3mol/L, described lemon
Acid solution is (30～50) with the mass ratio of mangrove bark powder: 1.
Preferably, step 2) described in the temperature of water-bath preheating be 60～80 DEG C, described water-bath preheating
Time is 2～8min.
Preferably, step 2) described in the power of ultrasonic extraction be 400～1000W, described ultrasonic carry
The temperature taken is 60～80 DEG C, and the time of described ultrasonic extraction is 20～40min.
Preferably, the separation of solid and liquid described in step 3 uses centrifugal mode, and described centrifugal rotating speed is
7000～9000rpm, the time is 5～15min.
Preferably, step 3) described in the volume ratio of liquid phase component and ethanol be 1: (1～3), ethanol
Volumetric concentration is 90～95%.
Beneficial effects of the present invention: the present invention effectively utilizes mango processing byproduct mangrove bark to prepare for raw material
Pectin, had both controlled pollution on the environment, had improve again the added value of mango secondary industry；This
The method that bright employing ultrasonic wave-temperature-organic acid combines, the pectin emulsifiability obtained is good, and recovery rate is high
(16.70-17.15%)；And the method process time of present invention offer is short, pectin viscosity is high, thickening property
And good stability；After the mango aqueous pectin solution of mass concentration 1% mixes with 20-40% soybean oil, homogeneous
Mango pectin emulsion after process, particle diameter ＜ 15um, place emulsion stability ＞ 80% after 7d, emulsion is steady
Qualitative it is significantly higher than business citrus pectin.
Accompanying drawing explanation
Fig. 1 is the viscosity viscosity contrast with citrus pectin of the mangrove bark pectin that the embodiment of the present invention obtains；
Fig. 2 is the grading curve figure of emulsion prepared by the mangrove bark pectin obtained with the embodiment of the present invention,
Fresh emulsion in wherein A has been homogeneous 5min；B is emulsion after preserving 7 days；
Fig. 3 is the microexamination of the particle diameter of emulsion prepared by the mangrove bark pectin obtained with the embodiment of the present invention,
Wherein A is citrus pectin emulsion；B-D is mango pectin emulsion；
Fig. 4 is emulsion storage-stable state diagram prepared by the mangrove bark pectin obtained with the embodiment of the present invention,
Pectin emulsion state figure in wherein A has been homogeneous 5min, B is 7 days consequence latex solution states
Figure.
Detailed description of the invention
The invention provides the preparation method of a kind of high emulsibility mangrove bark pectin, comprise the following steps:
1) by mangrove bark blanching, freeze-drying with pulverize and sieve, mangrove bark powder is obtained；
2) by described step 1) in the mangrove bark powder that obtains mix with citric acid solution after obtain mixed liquor,
Water-bath preheating mixed liquor, the pH value of regulation mixed liquor is 2.0～3.0, carries out ultrasonic extraction, is extracted
Liquid；
3) by described step 2) extract that obtains carries out separation of solid and liquid, described separation of solid and liquid obtained
Liquid phase component mixes with ethanol and precipitates, and obtains mangrove bark pectin.
The present invention prepares pectin with mangrove bark for raw material, and mangrove bark is discarded in mango product processing
Leftover bits and pieces.In the present invention, described mangrove bark is preferably the fresh mango skin in mango is processed 1～2 day.
Mangrove bark is carried out blanching by the present invention, preferably uses clear water to be carried out before blanching.In the present invention,
Described blanching is preferably steam blanching；Described mangrove bark is carried out the time of steam blanching preferably
1～3min, more preferably 2min.In the present invention, during described steam blanching, steam
High temperature can be passivated the endogenous enzymes in mangrove bark, thus prevents mangrove bark in follow-up processing procedure from occurring
Enzymatic reaction and affect pectin quality.
The present invention carries out freeze-drying after described mangrove bark carries out blanching.The present invention is preferably described cold
Lyophilized dry before, the mangrove bark after described blanching is carried out precooling；In the present invention, described precooling is preferably adopted
Carrying out with liquid nitrogen, the time of described precooling is preferably 2～10min, more preferably 6min.
Heretofore described cryodesiccated vacuum and temperature do not have particular/special requirement, use this area freezing
It is dried conventional vacuum and temperature；In the present invention, the described cryodesiccated time is preferably
36～60h, more preferably 40～55h, most preferably 48h.
Mangrove bark after described freeze-drying, after mangrove bark freeze-drying, is pulverized by the present invention.The present invention
The method of described pulverizing is not had special restriction, uses the technology of pulverizing well known to those skilled in the art
Scheme；Concrete embodiments of the invention can use micromill commonly used in the art to described cold
Freeze dried mangrove bark to pulverize.
After described pulverizing, the mangrove bark of described pulverizing is sieved by the present invention, obtains mangrove bark powder.At this
In bright, described in sieved preferably 20～60 mesh sieves, be more preferably 40～50 mesh sieves.
Currently preferred will sieve after mangrove bark powder be vacuum-packed, described vacuum packaging uses
Vacuum-packing method commonly used in the art, without other particular/special requirements；Currently preferred by vacuum packet
Mangrove bark powder after dress carries out refrigerating standby, and the temperature of described refrigeration is preferably 1～5 DEG C, more preferably
For 4 DEG C.After obtaining mangrove bark powder, described mangrove bark powder is mixed to get mixed with citric acid solution by the present invention
After closing liquid, it is carried out water-bath preheating.In the present invention, the concentration of described citric acid solution is preferably
0.1～0.3mol/L, more preferably 0.2mol/L；Described citric acid solution and the mass ratio of mangrove bark powder
Preferably (30～50): 1, more preferably 40: 1, currently preferred by mangrove bark powder and citric acid
After solution mixes, it is sealed in Polythene Bag, will be equipped with the poly-second of mangrove bark powder and citric acid solution
Alkene bag is put into and is carried out water-bath preheating in water-bath, and the temperature of described water-bath preheating is preferably 60～80 DEG C, more
Preferably 70 DEG C, the time of described water-bath preheating is preferably 2～8min, more preferably 5min.
The present invention is after water-bath preheating mixed liquor, and the pH value regulating described preextraction liquid is 2.0～3.0, enters
The ultrasonic extraction of row, obtains extract.The pH value of described preextraction liquid is preferably regulated to 2.5 by the present invention.
In the present invention, the solution used by pH value regulation is citric acid and sodium citrate, after the pH value of regulation mixed liquor,
The present invention carries out ultrasonic extraction to the mixed liquor after described regulation pH value, obtains extract.The present invention is to institute
State the ultrasonic instrument used that extracts and there is no special restriction, use well known to those skilled in the art ultrasonic broken
Broken instrument.In the present invention, the power of described ultrasonic extraction is preferably 400～1000W, more preferably
For 500～900W, most preferably 600～800W；The temperature of described ultrasonic extraction is preferably
60～80 DEG C, more preferably 70～80 DEG C, most preferably 80 DEG C；The time of described ultrasonic extraction is preferred
It is 20～40min, more preferably 25～35min, most preferably 30min.The present invention is by described ultrasonic
Extract and fully extract the pectin in mangrove bark powder.
Extract, after obtaining extract, is carried out separation of solid and liquid by the present invention, by the liquid phase component that obtains with
Ethanol mixing precipitates, and obtains mangrove bark pectin.The present invention does not has spy to the method for described separation of solid and liquid
Different restriction, uses the technical scheme of separation of solid and liquid well known to those skilled in the art；In the present invention
In, described separation of solid and liquid is preferably centrifugal；Described centrifugal rotating speed is preferably 7000～9000rpm,
More preferably 8000rpm；The described centrifugal time is preferably 5～15min, more preferably 10min.
The liquid phase component obtained, after extract is carried out separation of solid and liquid, is mixed with ethanol and carries out by the present invention
Precipitation, obtains mangrove bark pectin.In the present invention, described liquid phase component is preferred with the volume ratio of ethanol
It is 1: (1～3), more preferably 1: 2；The volumetric concentration of described ethanol is preferably 90～95%, more excellent
Choosing for 95%.In the present invention, the time carrying out precipitating described in is preferably 10～14h, more preferably
12h, long ethanol precipitation can make pectin fully separate out in the environment of ethanol.
Precipitation terminate after, currently preferred use volumetric concentration be 95% ethanol the precipitation obtained is entered
Row cyclic washing, obtains mangrove bark pectin.In the present invention, the number of times of described washing is preferably 2～5
Secondary, more preferably 3～4 times.The present invention precipitation to obtaining carries out ethanol washing, it is possible to remove pectin
The pigment of precipitation surface and free neutral sugar；After obtaining the precipitation of pectin, currently preferred general
Described precipitation carries out freeze-drying, obtains mangrove bark pectin.The present invention carries out freeze-drying to described precipitation
Condition without particular/special requirement, use the cryodesiccated condition that this area is conventional；In the present invention,
Described cryodesiccated temperature is preferably-10 DEG C～-50 DEG C, more preferably-20～-40 DEG C, most preferably
-25～-35 DEG C；Described cryodesiccated vacuum is preferably 1.3～13Pa, more preferably 2～10Pa.
The preparation method of the high emulsibility mangrove bark pectin provided the present invention below in conjunction with embodiment is carried out in detail
Thin explanation, but they can not be interpreted as limiting the scope of the present invention.
Embodiment 1
Fresh mango skin with clear water clean, steam blanching 2min to be passivated endogenous enzymes, Liquid nitrogen precooler 6min
And pulverize after carrying out freeze-drying 48h, cross 40 mesh sieves after pulverizing and obtain mangrove bark powder, vacuum packaging
4 DEG C of refrigerations it are put in standby after mangrove bark powder；Mix with 0.2mol/L citric acid solution with mangrove bark powder, lemon
Lemon acid solution is 40: 1 with the mass ratio of mangrove bark powder；After sodium citrate solution and mangrove bark powder are mixed close
It is encapsulated in Polythene Bag, Polythene Bag is placed in water-bath preheating 5min in 70 DEG C of water-baths, obtains preextraction
Liquid；Use citric acid and sodium citrate that the preextraction liquid pH obtained is adjusted to 2.5, put into power 600W
Ultrasound Instrument ultrasonic extraction 30min under the conditions of 70 DEG C, fully extract pectin, obtain extract；Will
The extract arrived is centrifugal 10min under the conditions of 8000rpm, adds 2 times in the liquid phase component collected
95% (v/v) ethanol precipitation 12h of liquid phase component volume, so that pectin fully separates out；Gained precipitation is used
The ethanol cyclic washing of 95% (v/v) 3 times, to remove the pigment in pectin precipitation and free neutral sugar；
The precipitation obtained is obtained mangrove bark pectin after vacuum freeze drying.
The yield calculating mangrove bark pectin obtained above is 16.70%；
The present invention uses viscosimeter to measure the viscosity of mangrove bark pectin, and result is as it is shown in figure 1, Fig. 1 is fruit
Adhesiveness is with shearing force change curve, and as seen from Figure 1, pectin solution viscosity is big, thickening property is good (attached
Fig. 1-mango pectin 1)；Emulsion particle diameter distribution prepared by the pectin that present invention detection obtains, result such as Fig. 2
Shown in Fig. 3, Fig. 2 is pectin emulsion particle diameter distribution curve, and Fig. 3 is the microscope of pectin emulsion particle diameter
Observe figure, by Fig. 2 and Fig. 3 it can be seen that the pectin product emulsion particle diameter ＜ 15 for preparing of the present embodiment
μm (accompanying drawing 2-mango pectin 1, accompanying drawing 3-B).
The mangrove bark pectin that mangrove bark pectin obtained above is configured to mass concentration 1% by the present invention is water-soluble
Liquid, the business citrus pectin aqueous solution with 1% is comparison, and described business citrus pectin is the most commercially available
Citrus pectin, after mangrove bark aqueous pectin solution and the comparison citrus pectin aqueous solution and the mixing of 40% soybean oil,
Homogenizer is used to obtain mango pectin emulsion and citrus pectin after homogeneous process 1min under 10000rpm
Emulsion, microscope is observed the particle diameter of pectin emulsion, is observed its stability, pectin emulsion state after placing 7 days
As shown in Figure 4, the pectin emulsion state figure in wherein A has been homogeneous 5min, B is pectin after 7 days
Emulsion state figure, it can be seen that, after 7d placed by mangrove bark pectin of the present invention, emulsion stability is good (attached
Fig. 4-mango pectin 1), stability of emulsion is significantly higher than business citrus pectin.
Embodiment 2
Fresh mango skin with clear water clean, steam blanching 2min to be passivated endogenous enzymes, Liquid nitrogen precooler 8min
And pulverize after carrying out freeze-drying 52h, cross 40 mesh sieves after pulverizing and obtain mangrove bark powder, vacuum packaging
4 DEG C of refrigerations it are put in standby after mangrove bark powder；Mix with 0.2mol/L citric acid solution with mangrove bark powder, lemon
Lemon acid solution is 30: 1 with the mass ratio of mangrove bark powder；After sodium citrate solution and mangrove bark powder are mixed close
It is encapsulated in Polythene Bag, is placed in 60 DEG C of water-baths preheating 6min and obtains preextraction liquid；Use citric acid
With sodium citrate, the preextraction liquid pH obtained is adjusted to 2.3, puts into the Ultrasound Instrument of power 800W in 60 DEG C
Under the conditions of process 20min, fully extract pectin and obtain extract, 7000rpm collects liquid after being centrifuged 8min
The extract that phase component obtains, adds 2 times of volume 95% ethanol precipitation 14h in the liquid phase component collected
So that pectin fully separates out, gained precipitation, with the ethanol cyclic washing 5 times of 95%, precipitates removing pectin
In pigment and free neutral sugar；The precipitation obtained is obtained mangrove bark pectin after vacuum freeze drying.
The yield calculating mangrove bark pectin obtained above is 16.90%；
The present invention uses viscosimeter to measure the viscosity of mangrove bark pectin, and result is as it is shown in figure 1, Fig. 1 is fruit
Adhesiveness is with shearing force change curve, and as seen from Figure 1, pectin solution viscosity is big, thickening property is good (attached
Fig. 1-mango pectin 2)；
The present invention detection obtain pectin emulsion particle diameter distribution, result as shown in Figures 2 and 3, Fig. 2
For pectin grading curve, Fig. 3 is pectin emulsion particle diameter microscope observation figure, can by Fig. 2 and Fig. 3
To find out particle diameter ＜ 15um (accompanying drawing 2-mango pectin 2, accompanying drawing 3-C)；
The mangrove bark pectin that mangrove bark pectin obtained above is configured to mass concentration 1% by the present invention is water-soluble
Liquid, the business citrus pectin aqueous solution with 1% is comparison, and described business citrus pectin is the most commercially available
Citrus pectin, after mangrove bark aqueous pectin solution and the comparison citrus pectin aqueous solution and the mixing of 40% soybean oil,
Homogenizer is used to obtain mango pectin emulsion and citrus fruit latex after homogeneous process 1min under 10000rpm
Liquid, the particle diameter of microscope observation pectin emulsion, observe its stability after placing 7 days, pectin emulsion state is such as
Shown in Fig. 4, the pectin emulsion state figure in wherein A has been homogeneous 5min, B is 7 days consequence latex
Liquid status figure, it can be seen that, after 7d placed by mangrove bark pectin of the present invention, emulsion stability is good
(accompanying drawing 4-mango pectin 2), stability of emulsion is significantly higher than business citrus pectin.
Embodiment 3
Fresh mango skin with clear water clean, steam blanching 2min to be passivated endogenous enzymes, Liquid nitrogen precooler 4min
And pulverize after carrying out freeze-drying 40h, cross 40 mesh sieves after pulverizing and obtain mangrove bark powder, vacuum packaging
4 DEG C of refrigerations it are put in standby after mangrove bark powder；Mix with 0.25mol/L citric acid solution with mangrove bark powder,
Citric acid solution is 50: 1 with the mass ratio of mangrove bark powder；After citric acid solution and mangrove bark powder are mixed close
It is encapsulated in Polythene Bag, is placed in preextraction 6min in 80 DEG C of water-baths and obtains preextraction liquid；Use lemon
The preextraction liquid pH obtained is adjusted to 2.8 by acid and sodium citrate, put into the Ultrasound Instrument of power 500W in
Process 40min under the conditions of 80 DEG C, fully extract pectin and obtain extract, after 9000rpm is centrifuged 12min
Collect the extract that liquid phase component obtains, in the liquid phase component collected, add 2 times of volume 95% ethanol
10h is so that pectin fully separates out precipitation, gained precipitation with 95% ethanol cyclic washing 4 times, with removing
Pigment in pectin precipitation and free neutral sugar；The precipitation obtained is obtained awns after vacuum freeze drying
Pericarp pectin.
The yield calculating mangrove bark pectin obtained above is 16.90%；
The present invention uses viscosimeter to measure the viscosity of mangrove bark pectin, and result is as it is shown in figure 1, Fig. 1 is fruit
Adhesiveness is with shearing force change curve, and as seen from Figure 1, pectin solution viscosity is big, thickening property is good (attached
Fig. 1-mango pectin 3)；
The present invention detection obtain pectin emulsion particle diameter distribution, result as shown in Figures 2 and 3, Fig. 2
For pectin emulsion particle diameter distribution curve, by Fig. 2 and Fig. 3 it can be seen that particle diameter ＜ 15um (accompanying drawing 2-awns
Pectin 3, accompanying drawing 3-D)；
The mangrove bark pectin that mangrove bark pectin obtained above is configured to mass concentration 1% by the present invention is water-soluble
Liquid, the business citrus pectin aqueous solution with 1% is comparison, and described business citrus pectin is the most commercially available
Citrus pectin, after mangrove bark aqueous pectin solution and the comparison citrus pectin aqueous solution and the mixing of 40% soybean oil,
Homogenizer is used to obtain mango pectin emulsion and citrus fruit latex after homogeneous process 1min under 10000rpm
Liquid, the particle diameter of microscope observation pectin emulsion, observe its stability after placing 7 days, pectin emulsion state is such as
Shown in Fig. 4, the pectin emulsion state figure in wherein A has been homogeneous 5min, B is 7 days consequence latex
Liquid status figure, it can be seen that, after 7d placed by mangrove bark pectin of the present invention, emulsion stability is good (attached
Fig. 4-mango pectin 3), stability of emulsion is significantly higher than business citrus pectin.
As seen from the above embodiment, the preparation method of the high emulsibility mangrove bark pectin that the present invention prepares
The mangrove bark pectin yield prepared is high, and pectin solution viscosity is big, thickening property good, and emulsion stability is good
(particle diameter ＜ 15um), stability of emulsion is significantly higher than business citrus pectin.
The above is only the preferred embodiment of the present invention, it is noted that general for the art
For logical technical staff, under the premise without departing from the principles of the invention, it is also possible to make some improvement and profit
Decorations, these improvements and modifications also should be regarded as protection scope of the present invention.
Claims (10)
1. a preparation method for high emulsibility mangrove bark pectin, comprises the following steps:
1) by mangrove bark blanching, freeze-drying with pulverize and sieve, mangrove bark powder is obtained；
2) by described step 1) in the mangrove bark powder that obtains mix with citric acid solution after obtain mixed liquor,
Water-bath preheating mixed liquor, the pH value of regulation mixed liquor is 2.0～3.0, carries out ultrasonic extraction, is extracted
Liquid；
3) by described step 2) extract that obtains carries out separation of solid and liquid, described separation of solid and liquid obtained
Liquid phase component mixes with ethanol and precipitates, and obtains mangrove bark pectin.
The preparation method of a kind of high emulsibility mangrove bark pectin the most according to claim 1, its feature
Be, step 1) described in blanching be steam blanching, the time of described blanching is 1～3min.
The preparation method of a kind of high emulsibility mangrove bark pectin the most according to claim 1, its feature
Be, step 1) described in sieve for cross 20～60 mesh sieves.
The preparation method of a kind of high emulsibility mangrove bark pectin the most according to claim 1, its feature
Be, step 2) described in the concentration of citric acid solution be 0.1～0.3mol/L, described citric acid solution
It is (30～50) with the mass ratio of mangrove bark powder: 1.
The preparation method of a kind of high emulsibility mangrove bark pectin the most according to claim 1, its feature
Be, step 2) described in the temperature of water-bath preheating be 60～80 DEG C, the time of described preheating is
2～8min.
The preparation method of a kind of high emulsibility mangrove bark pectin the most according to claim 1, its feature
Be, step 2) described in the power of ultrasonic extraction be 400～1000W, the temperature of described ultrasonic extraction
Degree is 60～80 DEG C, and the time of described ultrasonic extraction is 20～40min.
The preparation method of a kind of high emulsibility mangrove bark pectin the most according to claim 1, its feature
Be, step 4) described in separation of solid and liquid be centrifugal, described centrifugal rotating speed is 7000～9000rpm,
The described centrifugal time is 5～15min.
The preparation method of a kind of high emulsibility mangrove bark pectin the most according to claim 1, its feature
Be, step 4) described in the volume ratio of liquid phase component and ethanol be 1: (1～3).
The preparation method of a kind of high emulsibility mangrove bark pectin the most according to claim 8, its feature
Being, the volumetric concentration of described ethanol is 90～95%.
10. according to the preparation of a kind of high emulsibility mangrove bark pectin described in claim 1～9 any one
Method, it is characterised in that described step 4) be precipitated after also include: by step 4) in obtain heavy
Shallow lake carries out vacuum freeze drying, obtains mangrove bark pectin.
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| CN106866839A (en) * | 2017-03-13 | 2017-06-20 | 浙江大学 | The method that ultrasonic hot acid method extracts LM in Hu shaddock ped |
| CN110699405A (en) * | 2019-11-20 | 2020-01-17 | 江南大学 | Method for preparing mango peel pectin oligosaccharides with antibacterial activity by using immobilized polygalacturonase |
| CN111349183A (en) * | 2020-04-22 | 2020-06-30 | 黑龙江八一农垦大学 | Pectin and its extraction method and preparation method |
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| CN112056544A (en) * | 2020-09-21 | 2020-12-11 | 华中农业大学 | Preparation method of pectin emulsion gel capable of stably loading fat-soluble active ingredients |
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| CN114752001A (en) * | 2022-04-11 | 2022-07-15 | 华中农业大学 | High-emulsibility persimmon pectin and preparation method and application thereof |
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