CN105777224A - Ganoderma lucidum karst culture medium and preparation method and application thereof - Google Patents

Ganoderma lucidum karst culture medium and preparation method and application thereof Download PDF

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Publication number
CN105777224A
CN105777224A CN201610067707.3A CN201610067707A CN105777224A CN 105777224 A CN105777224 A CN 105777224A CN 201610067707 A CN201610067707 A CN 201610067707A CN 105777224 A CN105777224 A CN 105777224A
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ganoderma lucidum
medium
fructus hordei
hordei germinatus
ganoderma
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CN201610067707.3A
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李玉平
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Individual
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    • CCHEMISTRY; METALLURGY
    • C05FERTILISERS; MANUFACTURE THEREOF
    • C05FORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00Other organic fertilisers

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Mushroom Cultivation (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

The invention discloses a ganoderma lucidum karst culture medium and a preparation method and application thereof.The ganoderma lucidum karst culture medium is prepared from one or more of fructus hordei germinatus, corn and corncobs, soybeans and purified water.The ganoderma lucidum karst culture medium prepared through the method is suitable for culture of various ganoderma lucidum karst and particularly suitable for ganoderma lucidum karst culture.The content of nutritional ingredients of ganoderma lucidum karst can be greatly increased, the quality of cultured ganoderma lucidum karst can be changed and greatly improved, the content of the nutritional ingredients of the cultured ganoderma lucidum karst can be changed and greatly increased, the cultured ganoderma lucidum karst is more suitable for comprehensive utilization, and the prospect of deep processing is wider.In addition, by means of the culture medium, secondary growth of the ganoderma lucidum karst can be continuously met after primary growth of the ganoderma lucidum karst is met; rich nutritional substances in the culture medium can be absorbed better and more completely during the secondary growth period; after the secondary growth period, the yield of the ganoderma lucidum karst can be greatly increased, the quality is better, and the nutritional ingredients are better.

Description

A kind of Medium for Ganoderma lucidum and preparation method thereof and application
Technical field
The present invention relates to cultivation of glossy ganoderma field, particularly to a kind of Medium for Ganoderma lucidum and preparation method thereof and application.
Background technology
Culture medium is the place of the various nutrient substance that Ganoderma depends on for existence, and when using kind identical with production environment condition, the quality of culture medium will directly affect the quality of Ganoderma.At present, the cultivation of glossy ganoderma culture medium that China is traditional is all with traditional basswood, wood flour, cotton seed hull, corn cob, wheat bran etc. for raw material.As the culture medium of Ganoderma, traditional growing and cultivation culture medium is applicable to plucking and viewing and admiring of Ganoderma finished product, also exists that nutrient composition content is low and whole synthesis interest rate and the drawback such as deep processing is limited.
Ganoderma, formal name used at school: GanodermaLucidumKarst, belong to the edible fungi of Basidiomycotina.The discovery of Ganoderma and application, in the history of the existing more than one thousand years of China.Modern medical theory is studied and all has turned out clinically: Ganoderma has resisting fatigue, improves the effects such as immunity, antitumor, invigorating QI and tranquilization, relieving cough and asthma.It is applied to dizziness sleeplessness, shortness of breath and palpitation, asthenia cough with asthma etc. at present.Owing to effect of Ganoderma is notable, therefore we have realized artificial growth at present, to meet more and more different crowd needs to Ganoderma.But, in cultivation, tradition can not meet, with the culture medium that basswood, wood flour, cotton seed hull, corn cob, wheat bran etc. are raw material, the problem that production nutrient composition content is high and whole synthesis utilization rate is high.
Summary of the invention
The technical problem to be solved is to provide a kind of Medium for Ganoderma lucidum and preparation method thereof and application, which overcomes the disadvantages mentioned above of prior art.
The technical problem to be solved is achieved through the following technical solutions:
A kind of Medium for Ganoderma lucidum, is prepare by including following raw material: one or more compositions of Fructus Hordei Germinatus, Semen Maydis or corn cob, Semen Glycines and pure water.
Preferably, in technique scheme, described Medium for Ganoderma lucidum includes following parts by weight of component: one or more compositions 60-450 of Fructus Hordei Germinatus, Semen Maydis and corn cob, Semen Glycines 10-50 and pure water 50-400, wherein, when compositions is Fructus Hordei Germinatus, Semen Maydis and three kinds of raw materials of corn cob, its ratio is 2:1:1-1:1:1.
Preferably, in technique scheme, described Medium for Ganoderma lucidum is following parts by weight of component: one or more compositions 100-200 of Fructus Hordei Germinatus, Semen Maydis and corn cob, Semen Glycines 15-30 and pure water 60-150, wherein, when compositions is Fructus Hordei Germinatus, Semen Maydis and three kinds of raw materials of corn cob, its ratio is 1:1:1.
Preferably, in technique scheme, described Medium for Ganoderma lucidum is following parts by weight of component: one or more compositions 140 of Fructus Hordei Germinatus, Semen Maydis and corn cob, Semen Glycines 15 and pure water 90, wherein, when compositions is Fructus Hordei Germinatus, Semen Maydis and three kinds of raw materials of corn cob, its ratio is 1:1:1.
Preferably, in technique scheme, described Fructus Hordei Germinatus is fresh Fructus Hordei Germinatus and/or dried malt.
The preparation method of a kind of Medium for Ganoderma lucidum, comprises the following steps:
(1) one or more compositions of the Fructus Hordei Germinatus of above-mentioned formula, Semen Maydis or corn cob is mixed with Semen Glycines put in pure water, pulverize after stirring, obtain compound;
(2) compound subpackage step (1) stirred, obtains culture medium.
Preferably, in technique scheme, the time of the stirring of described step (1) is 20-40min.
The application of a kind of Medium for Ganoderma lucidum, described application includes a secondary growth and the diauxic growth of Ganoderma.
Preferably, in technique scheme, described diauxic growth carries out according to following steps: after being plucked by the Ganoderma of growth and maturity, more free of contamination culture medium is cut into irregular bulk, the culture medium being cut into bulk is put into uncovered container and carries out the diauxic growth of Ganoderma.
Technique scheme of the present invention, has the advantages that
The invention provides a kind of Medium for Ganoderma lucidum and preparation method thereof, which obviate the culture medium that traditional basswood, wood flour, cotton seed hull, corn cob, wheat bran etc. are raw material, its preparation method is simpler, and raw material is drawn materials and is more prone to, lower in cost.Most importantly, by culture medium prepared by the method, it is applicable to the cultivation of various Ganodermas, and is particularly suitable for the cultivation of Ganoderma.It can be greatly improved the nutrient composition content of Ganoderma, and its Ganoderma cultivated can change and be greatly improved quality and the nutrient composition content of Ganoderma, is more suitable for comprehensive utilization, and the prospect of deep processing is wider.Meanwhile, the present invention also provides new cultivation technique means for Ganoderma, makes current existing Ganoderma artificial cultivation technique be expanded.
Additionally, the culture medium of the present invention, after meeting a secondary growth of Ganoderma, can continue to meet the diauxic growth of Ganoderma.The mode of this diauxic growth, can make in a growth course, the raw material all do not used up in culture medium is absorbed fully and is recycled, and the culture medium of diauxic growth adopts the culture medium of a secondary growth completely, it does not have add new raw material, it is entirely the raw material not used completely in a secondary growth, save to the full extent and make use of raw material, save the cost of large-scale production simultaneously, operation is also more prone to, it is not necessary to put into extra human and material resources and financial resources.Diauxic growth can absorb nutrient substance abundant in culture medium better, more completely.Effective raw material in diauxic growth, culture medium is fully used, and meanwhile, the yield of Ganoderma can be greatly improved, and quality is higher and nutritional labeling is better.
Detailed description of the invention
Below the specific embodiment of the present invention is described in detail, in order to be further appreciated by the present invention.
Embodiment 1
A kind of Medium for Ganoderma lucidum, is be prepared from by following raw material components (kg): dried malt and/or fresh Fructus Hordei Germinatus 40, Semen Maydis 20, corn cob 20, Semen Glycines 10 and pure water 50.
Above-mentioned Medium for Ganoderma lucidum is prepared by following steps:
(1) putting in pure water by Fructus Hordei Germinatus, Semen Maydis, Semen Glycines, corn cob mixing, stirring 20-40min uniformly pulverizes afterwards, obtains compound;
(2) compound subpackage step (1) stirred, obtains culture medium.
Embodiment 2
A kind of Medium for Ganoderma lucidum, is be prepared from by following raw material components (kg): dried malt and/or fresh Fructus Hordei Germinatus 150, Semen Maydis 150, corn cob 150, Semen Glycines 50 and pure water 400.
Its manufacture method is with embodiment 1.
Embodiment 3
A kind of Medium for Ganoderma lucidum, is be prepared from by following raw material components (kg): dried malt and/or fresh Fructus Hordei Germinatus 50, Semen Maydis 25, corn cob 25, Semen Glycines 15 and pure water 60.
Its manufacture method is with embodiment 1.
Embodiment 4
A kind of Medium for Ganoderma lucidum, is be prepared from by following raw material components (kg): dried malt and/or fresh Fructus Hordei Germinatus 100, Semen Maydis 50, corn cob 50, Semen Glycines 30 and pure water 150.
Its manufacture method is with embodiment 1.
Embodiment 5
A kind of Medium for Ganoderma lucidum, is be prepared from by following raw material components (kg): dried malt and/or fresh Fructus Hordei Germinatus 70, Semen Maydis 35, corn cob 35, Semen Glycines 15 and pure water 90.
Its manufacture method is with embodiment 1.
Embodiment 6
A kind of Medium for Ganoderma lucidum, is be prepared from by following raw material components (kg): dried malt and/or fresh Fructus Hordei Germinatus 140, Semen Glycines 15 and pure water 90.
Above-mentioned Medium for Ganoderma lucidum is prepared by following steps:
(1) putting in pure water by Fructus Hordei Germinatus, stirring 20-40min uniformly pulverizes afterwards;
(2) compound subpackage step (1) stirred, obtains culture medium.
Embodiment 7
A kind of Medium for Ganoderma lucidum, is be prepared from by following raw material components (kg): Semen Maydis 140, Semen Glycines 15 and pure water 90.
Its manufacture method is similar to embodiment 6, wherein the Fructus Hordei Germinatus of embodiment 6 is replaced by Semen Maydis.
Embodiment 8
A kind of Medium for Ganoderma lucidum, is be prepared from by following raw material components (kg): corn cob 140, Semen Glycines 15 and pure water 90.
Its manufacture method is similar to embodiment 6, wherein the Fructus Hordei Germinatus of embodiment 6 is replaced by corn cob.
Embodiment 9
A kind of Medium for Ganoderma lucidum, is be prepared from by following raw material components (kg): dried malt and/or fresh Fructus Hordei Germinatus 90, Semen Maydis 50, Semen Glycines 15 and pure water 90.
Its manufacture method, with embodiment 1, only removes corn cob.
Embodiment 10
A kind of Medium for Ganoderma lucidum, is be prepared from by following raw material components (kg): dried malt and/or fresh Fructus Hordei Germinatus 90, corn cob 50, Semen Glycines 15 and pure water 90.
Its manufacture method, with embodiment 1, only removes Semen Maydis.
Embodiment 11
A kind of Medium for Ganoderma lucidum, is be prepared from by following raw material components (kg): Semen Maydis 70, corn cob 70, Semen Glycines 15 and pure water 90.
Its manufacture method, with embodiment 1, only removes Fructus Hordei Germinatus.
By the culture medium that the method for above-described embodiment prepares, it is to avoid culture medium that traditional basswood, wood flour, cotton seed hull, corn cob, wheat bran etc. are raw material, its preparation method is simpler.Most importantly, by culture medium prepared by the method, it is applicable to the cultivation of various Ganodermas, and is particularly suitable for the cultivation of Ganoderma.It can be greatly improved the nutrient composition content of Ganoderma, and its Ganoderma cultivated can change and be greatly improved quality and the nutrient composition content of Ganoderma, is more suitable for comprehensive utilization, and the prospect of deep processing is wider.Also provide new cultivation technique means for Ganoderma, make current existing Ganoderma artificial cultivation technique be expanded.
Present invention also offers the application of a kind of Medium for Ganoderma lucidum, this application includes a secondary growth and the diauxic growth of Ganoderma.
An above-mentioned secondary growth refers to a secondary growth of routine, cultivates the process obtaining sporophore.Above-mentioned diauxic growth refers to after the Ganoderma of growth and maturity being plucked, more free of contamination culture medium is cut into irregular bulk, by be cut into bulk, culture medium not of uniform size puts into uncovered container and carries out the diauxic growth of Ganoderma.
The culture medium prepared by said method of the present invention, it is provided that the nutrition required for Ganoderma two secondary growth.That is, after meeting a secondary growth, diauxic growth is continued.The mode of this diauxic growth, raw material unexhausted in culture medium can be made to be absorbed fully and utilize, and the culture medium of diauxic growth adopts the culture medium of a secondary growth completely, do not add new raw material, it is entirely the raw material not used completely in a secondary growth, and diauxic growth can absorb nutrient substance abundant in culture medium better, more completely.Through diauxic growth, the yield of Ganoderma can be greatly improved, and quality is higher and nutritional labeling is better.
Although the present invention is with embodiment openly as above; so it is not intended to limit the present invention, any those skilled in the art, without departing from the spirit and scope of the present invention; all can doing various different selection and amendment, therefore protection scope of the present invention is limited by claims and equivalents thereof.

Claims (9)

1. a Medium for Ganoderma lucidum, it is characterised in that described culture medium is to prepare by including following raw material components: one or more compositions of Fructus Hordei Germinatus, Semen Maydis and corn cob, Semen Glycines and pure water.
2. Medium for Ganoderma lucidum according to claim 1, it is characterized in that, described raw material is following parts by weight of component: one or more compositions 80-450 of Fructus Hordei Germinatus, Semen Maydis and corn cob, Semen Glycines 10-50 and pure water 50-400, wherein, when being raw material when selecting Fructus Hordei Germinatus, Semen Maydis and corn cob compositions, its ratio is 3:1:1-1:1:1.
3. Medium for Ganoderma lucidum according to claim 2, it is characterized in that, described raw material is following parts by weight of component: one or more compositions 100-200 of Fructus Hordei Germinatus, Semen Maydis and corn cob, Semen Glycines 15-30 and pure water 60-150, wherein, when being raw material when selecting Fructus Hordei Germinatus, Semen Maydis and corn cob compositions, its ratio is 2:1:1-1:1:1.
4. Medium for Ganoderma lucidum according to claim 3, it is characterized in that, described raw material is following parts by weight of component: one or more compositions 140 of Fructus Hordei Germinatus, Semen Maydis and corn cob, Semen Glycines 15 and pure water 90, wherein, when being raw material when selecting Fructus Hordei Germinatus, Semen Maydis and corn cob compositions, its ratio is 2:1:1.
5. the Medium for Ganoderma lucidum according to claim 1,2,3 or 4, it is characterised in that described Fructus Hordei Germinatus is fresh Fructus Hordei Germinatus and/or dried malt.
6. the preparation method according to the arbitrary described Medium for Ganoderma lucidum of claim 1-4, it is characterised in that described preparation method comprises the following steps:
(1) one or more compositions of the Fructus Hordei Germinatus of above-mentioned formula, Semen Maydis or corn cob is mixed with Semen Glycines put in pure water, pulverize after stirring, obtain compound;
(2) compound subpackage step (1) stirred, obtains culture medium.
7. the preparation method of Medium for Ganoderma lucidum according to claim 5, it is characterised in that the time of the stirring of described step (1) is 20-40min.
8. the application according to the arbitrary described Medium for Ganoderma lucidum of claim 1-4, it is characterised in that described application includes a secondary growth and the diauxic growth of Ganoderma.
9. the application of Medium for Ganoderma lucidum according to claim 8, it is characterized in that, described diauxic growth carries out according to following steps: after being plucked by the Ganoderma of growth and maturity, again free of contamination culture medium is cut into irregular bulk, the culture medium being cut into bulk is put into uncovered container and carries out the diauxic growth of Ganoderma.
CN201610067707.3A 2016-02-01 2016-02-01 Ganoderma lucidum karst culture medium and preparation method and application thereof Pending CN105777224A (en)

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107223483A (en) * 2017-07-24 2017-10-03 福建省农业科学院生物技术研究所 A kind of strawberry disease prevention cultivation matrix and preparation method thereof

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104058802A (en) * 2014-06-27 2014-09-24 朱正来 Ganoderma lucidum culture medium, method for cultivating ganoderma lucidum, ganoderma lucidum tea and preparation method of ganoderma lucidum tea
CN104876706A (en) * 2015-05-18 2015-09-02 张家港市鸿嘉数字科技有限公司 Lucid ganoderma culture medium

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104058802A (en) * 2014-06-27 2014-09-24 朱正来 Ganoderma lucidum culture medium, method for cultivating ganoderma lucidum, ganoderma lucidum tea and preparation method of ganoderma lucidum tea
CN104876706A (en) * 2015-05-18 2015-09-02 张家港市鸿嘉数字科技有限公司 Lucid ganoderma culture medium

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107223483A (en) * 2017-07-24 2017-10-03 福建省农业科学院生物技术研究所 A kind of strawberry disease prevention cultivation matrix and preparation method thereof

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