CN105767910A - Method for fermentation processing of fermented soybeans highly rich in gamma-aminobutyric acid - Google Patents
Method for fermentation processing of fermented soybeans highly rich in gamma-aminobutyric acid Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 22
- BTCSSZJGUNDROE-UHFFFAOYSA-N gamma-aminobutyric acid Chemical compound NCCCC(O)=O BTCSSZJGUNDROE-UHFFFAOYSA-N 0.000 title abstract description 32
- 229960003692 gamma aminobutyric acid Drugs 0.000 title abstract description 27
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- 238000012545 processing Methods 0.000 title abstract description 6
- OGNSCSPNOLGXSM-UHFFFAOYSA-N (+/-)-DABA Natural products NCCC(N)C(O)=O OGNSCSPNOLGXSM-UHFFFAOYSA-N 0.000 title abstract description 5
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- GOMNOOKGLZYEJT-UHFFFAOYSA-N isoflavone Chemical compound C=1OC2=CC=CC=C2C(=O)C=1C1=CC=CC=C1 GOMNOOKGLZYEJT-UHFFFAOYSA-N 0.000 description 1
- CJWQYWQDLBZGPD-UHFFFAOYSA-N isoflavone Natural products C1=C(OC)C(OC)=CC(OC)=C1C1=COC2=C(C=CC(C)(C)O3)C3=C(OC)C=C2C1=O CJWQYWQDLBZGPD-UHFFFAOYSA-N 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The invention relates to a method for fermentation processing of fermented soybeans highly rich in gamma-aminobutyric acid.The method comprises the steps of 1, decoction soaking; 2, steaming, wherein soaked black soybeans obtained in the step 1 are placed in a steamer to be steamed for 1-2 h separated from water, then taken out to be dried and placed in a container, and the surface of the steamed black soybeans is covered with decocted folium mori residues and sweet wormwood herb residues; 3, fermentation processing; 4, finished product obtaining, wherein a product obtained in the step 3 through fermentation processing is placed in the steam to be steam for 0.4-0.5 h separated from water and then dried to obtain the finished fermented soybeans.The fermentation processing steps are simple, the GABA content in the processed fermented soybeans is as high as 21.65-22.43 mg/g, and therefore the fermented soybeans become a new resource for people to take GABA and the ever increasing demand of consumers for safe and cheap functional foods can be well met; meanwhile, the advantages of traditional Chinese medicine can be utilized, and the clinical application range of fermented soybeans can be widened.
Description
Technical field
The present invention relates to the Semen Sojae Preparatum fermentation concocting method of a kind of high enriching gamma-aminobutyric.
Background technology
Research display γ-aminobutyric acid (γ-amino butyric acid, GABA) it is the primary inhibitory neurotransmitter of mammalian central nervous system, has multiple important physiological function: have and fundamentally play calmness, anxiety, hypermnesis and improve the usefulness of sleep；Brain-invigorating, the effect of reducing blood pressure, treatment neurodegenerative diseases, Control of asthma, treatment epilepsy, defying age, improve hepatic and renal function, alleviate arteriosclerosis, prevent obesity, improve diabetes and the multiple efficacies such as climacteric syndrome, antitumor, have been reported that GABA can be used for treating drug addiction.Along with going deep into of research, the physiological function of GABA is continuously available and illustrates, and has been developed as a kind of novel functional component, is the most gradually applied to the industry such as medicine, food.GABA is widely distributed, all has existence, but in the case of nature, content is extremely low in animal, plant and microorganism.From natural food, the GABA of picked-up can not meet far away the daily demand of human body.
Having multiple important physiological function and wide application prospect based on GABA, from 20th century the mid-80, the most many scholars so far pay attention to researching and developing the functional food rich in GABA.Develop the numerous food rich in GABA at present, such as GABARON TEA, Rice plumule and Testa oryzae, sprout brown rice, germination bean product, wine and beverage etc., and the high GABA content health food material etc. fermented with lactic acid bacteria, yeast, aspergillosis.They all directly as or for blood pressure lowering, improve the functional food such as sleep, antidepressant, blood sugar lowering.Utilize the functional food ingredient rich in GABA that the development of raw materials such as Rice plumule manufacture such as Japanese Scientists, oneself is through being widely used in the goods such as beverage, fruit jam, cake, cookies, flavouring agent.Calendar year 2001 China's Ministry of Public Health approval GABA is classified as new resource food and processes for food production.
Semen Sojae Preparatum (Sojae Semen Praeparatum) is to commonly use Chinese medicine simply, for legume soybean Glycine max(L) the fermentation processing product of the mature seed of Merr, with Black seed coat strain Semen sojae atricolor as primary raw material, Folium Mori, Herba Artemisiae Annuae are that adjuvant is equipped with the most fermented process of preparing Chinese medicine and forms.Semen Sojae Preparatum begins to be loaded in " Mingyi Bielu ", history tree and to go through version " Chinese Pharmacopoeia " the most on the books.As Chinese medicine application at least more than 1700 years history, effect and cure mainly deep clinical practice basis.Function with cure mainly: induce sweat, relieving restlessness, a surname send out hot and suffocating.For catching a cold, cold and heat headache, irritated uncomfortable in chest, restlessness of asrhenia type and insomnia etc..Semen Sojae Preparatum is put into the Dietotherapy health Chinese medicine of the first batch of dietotherapeutic of Ministry of Public Health.It is now recognized that Semen Sojae Preparatum main active is soybean isoflavone, do not pay close attention to the existence of GABA in Semen Sojae Preparatum.
There is no the Semen Sojae Preparatum concocting method of document report height enrichment GABA at present.Therefore the Semen Sojae Preparatum fermentation concocting method of a kind of high enriching gamma-aminobutyric it is badly in need of.
Summary of the invention
For solving above-mentioned technical problem, the present invention provides the Semen Sojae Preparatum fermentation concocting method of a kind of high enriching gamma-aminobutyric.
The Semen Sojae Preparatum fermentation concocting method of the high enriching gamma-aminobutyric of the present invention, comprises the following steps:
1) medicine juice soaks, take the crude drug of following parts by weight: Folium Mori and Herba Artemisiae Annuae each 70-100 part, join in the water of crude drug amount 16-20 times and decoct 50-70 minute, filter, repeat to decoct three times, merge three decocting liquids, decocting liquid is concentrated into 1000ml, clean Semen sojae atricolor 1000 parts is admixed in decocting liquid and soak 5.5-6.5 hour, after decocting liquid exhausts, so that beans is the most glutted；
2) steam: the Semen sojae atricolor after step 1) is soaked is placed in steamer, Steam by water bath 1-2 hour, dry moisture after taking-up, then be placed in bamboo weaving container, cover the Semen sojae atricolor surface after steaming with the Folium Mori slag decocted and Herba Artemisiae Annuae slag；
3) fermentation is concocted: by step 2) in container Semen sojae atricolor put into temperature be 28-32 DEG C, humidity be that the incubator of 60-80% carries out natural fermentation, timing is stirred, ferment 5-7 days, make, all over Planta La flavescens (Fabricius)． on Semen sojae atricolor surface, to take out, remove Folium Mori slag and Herba Artemisiae Annuae slag, wash away Planta La flavescens (Fabricius)．, being placed in ceramic vessel with cover, Folium Mori slag and Herba Artemisiae Annuae slag cover, and lid ceramic vessel lid also uses water-stop；Be subsequently placed in temperature be 28-32 DEG C, humidity be the most vexed 12-15 days of the incubator of 60-80%, to fully fermentation, take out when fragrance overflows；
4) finished product: the goods concocted through step 3) fermentation are placed in steamer, Steam by water bath 0.4-0.5 hour, dry and be Semen Sojae Preparatum finished product.
The Semen Sojae Preparatum fermentation concocting method of the high enriching gamma-aminobutyric of the present invention, in step 3): every 3 days of the most vexed period poured out goods, fully stirred.
Compared with prior art the invention have the benefit that the Semen Sojae Preparatum fermentation concocting method step of the high enriching gamma-aminobutyric of the present invention is simple, in the Semen Sojae Preparatum processed, GABA content is up to 21.65-22.43mg/g, GABA content is high, Semen Sojae Preparatum is made to become the new resources of picked-up GABA, can greatly meet the demand that consumer is growing to edible safety, cheap functional food, the advantage of China's Chinese medicine can be played simultaneously, and widen the clinical application range of Semen Sojae Preparatum.
Detailed description of the invention
Below in conjunction with embodiment, the detailed description of the invention of the present invention is described in further detail.Following example are used for illustrating the present invention, but are not limited to the scope of the present invention.
Embodiment 1
The Semen Sojae Preparatum fermentation concocting method of a kind of high enriching gamma-aminobutyric, comprises the following steps:
1) medicine juice soaks, take the crude drug of following weight (gram): Folium Mori and Herba Artemisiae Annuae each 70-100 part, join in the water of crude drug amount 16 times and decoct 50 minutes, filter, repeat to decoct three times, merge three decocting liquids, decocting liquid is concentrated into 1000ml, clean Semen sojae atricolor 1000 grams is admixed in decocting liquid and soak 5.5 hours, after decocting liquid exhausts, so that beans is the most glutted；
2) steam: the Semen sojae atricolor after step 1) is soaked is placed in steamer, Steam by water bath 2 hours, dry moisture after taking-up, then be placed in bamboo weaving container, cover the Semen sojae atricolor surface after steaming with the Folium Mori slag decocted and Herba Artemisiae Annuae slag；
3) fermentation is concocted: by step 2) in container Semen sojae atricolor put into temperature be 28 DEG C, humidity be 60% incubator carry out natural fermentation, timing is stirred, ferment 6 days, make, all over Planta La flavescens (Fabricius)． on Semen sojae atricolor surface, to take out, remove Folium Mori slag and Herba Artemisiae Annuae slag, wash away Planta La flavescens (Fabricius)．, being placed in ceramic vessel with cover, Folium Mori slag and Herba Artemisiae Annuae slag cover, and lid ceramic vessel lid also uses water-stop；Be subsequently placed in temperature be 28 DEG C, humidity be 60% the most vexed 12 days of incubator, to fully fermentation, take out when fragrance overflows；
4) finished product: the goods concocted through step 3) fermentation are placed in steamer, Steam by water bath 0.4 hour, dry and be Semen Sojae Preparatum finished product.
The Semen Sojae Preparatum fermentation concocting method of the high enriching gamma-aminobutyric of the present invention, in step 3): every 3 days of the most vexed period poured out goods, fully stirred.
Embodiment 2
The Semen Sojae Preparatum fermentation concocting method of a kind of high enriching gamma-aminobutyric, comprises the following steps:
1) medicine juice soaks, take the crude drug of following parts by weight: Folium Mori and Herba Artemisiae Annuae each 70-100 part, join in the water of crude drug amount 18 times and decoct 60 minutes, filter, repeat to decoct three times, merge three decocting liquids, decocting liquid is concentrated into 1000ml, clean Semen sojae atricolor 1000 grams is admixed in decocting liquid and soak 6 hours, after decocting liquid exhausts, so that beans is the most glutted；
2) steam: the goods after step 1) is soaked are placed in steamer, Steam by water bath 2 hours, dry moisture after taking-up, then be placed in bamboo weaving container, cover the Semen sojae atricolor surface after steaming with the Folium Mori slag decocted and Herba Artemisiae Annuae slag；
3) fermentation is concocted: by step 2) in container Semen sojae atricolor put into temperature be 30 DEG C, humidity be 70% incubator carry out natural fermentation, timing is stirred, ferment 6 days, make, all over Planta La flavescens (Fabricius)． on Semen sojae atricolor surface, to take out, remove Folium Mori slag and Herba Artemisiae Annuae slag, wash away Planta La flavescens (Fabricius)．, being placed in ceramic vessel with cover, Folium Mori slag and Herba Artemisiae Annuae slag cover, and lid ceramic vessel lid also uses water-stop；Be subsequently placed in temperature be 30 DEG C, humidity be 70% the most vexed 13 days of incubator, to fully fermentation, take out when fragrance overflows；
4) finished product: the Semen sojae atricolor concocted through step 3) fermentation is placed in steamer, Steam by water bath 0.4 hour, dries and be Semen Sojae Preparatum finished product.
The Semen Sojae Preparatum fermentation concocting method of the high enriching gamma-aminobutyric of the present invention, in step 3): every 3 days of the most vexed period poured out goods, fully stirred.
Embodiment 3
The Semen Sojae Preparatum fermentation concocting method of a kind of high enriching gamma-aminobutyric, comprises the following steps:
1) medicine juice soaks, take the crude drug of following weight (gram): Folium Mori and Herba Artemisiae Annuae each 70-100 part, join in the water of crude drug amount 20 times and decoct 70 minutes, filter, repeat to decoct three times, merge three decocting liquids, decocting liquid is concentrated into 1000ml, clean Semen sojae atricolor 1000 grams is admixed in decocting liquid and soak 6.5 hours, after decocting liquid exhausts, so that beans is the most glutted；
2) steam: the Semen sojae atricolor after step 1) is soaked is placed in steamer, Steam by water bath 2 hours, dry moisture after taking-up, then be placed in bamboo weaving container, cover the Semen sojae atricolor surface after steaming with the Folium Mori slag decocted and Herba Artemisiae Annuae slag；
3) fermentation is concocted: by step 2) in container Semen sojae atricolor put into temperature be 32 DEG C, humidity be 80% incubator carry out natural fermentation, timing is stirred, ferment 7 days, make, all over Planta La flavescens (Fabricius)． on Semen sojae atricolor surface, to take out, remove Folium Mori slag and Herba Artemisiae Annuae slag, wash away Planta La flavescens (Fabricius)．, being placed in ceramic vessel with cover, Folium Mori slag and Herba Artemisiae Annuae slag cover, and lid ceramic vessel lid also uses water-stop；Be subsequently placed in temperature be 32 DEG C, humidity be 80% the most vexed 15 days of incubator, to fully fermentation, take out when fragrance overflows；
4) finished product: the goods concocted through step 3) fermentation are placed in steamer, Steam by water bath 0.5 hour, dry and be Semen Sojae Preparatum finished product.
The Semen Sojae Preparatum fermentation concocting method of the high enriching gamma-aminobutyric of the present invention, in step 3): every 3 days of the most vexed period poured out goods, fully stirred.
Use the present invention high enriching gamma-aminobutyric Semen Sojae Preparatum fermentation Semen Sojae Preparatum prepared by concocting method in the extraction of γ-aminobutyric acid and mensuration comprise the following steps: Semen Sojae Preparatum finished product drying, pulverize, sieve.By the methanol aqueous solution supersound extraction of 10%, HPLC-ELSD measures sample alpha-aminobutyric acid content and is up to 21.65-22.43mg/g, GABA content is high, Semen Sojae Preparatum is made to become the new resources of picked-up GABA, also can extract the GABA in Semen Sojae Preparatum simultaneously, can keep the safety in production for food or the edible GABA that is considered natural additive, can greatly meet the demand that consumer is growing to edible safety, cheap functional food, the advantage of China's Chinese medicine can be played simultaneously, and widen the clinical application range of Semen Sojae Preparatum.
The above is only the preferred embodiment of the present invention; it should be pointed out that, for those skilled in the art, on the premise of without departing from the technology of the present invention principle; can also make some improvement and modification, these improve and modification also should be regarded as protection scope of the present invention.
Claims (2)
1. the Semen Sojae Preparatum fermentation concocting method of a high enriching gamma-aminobutyric, it is characterised in that comprise the following steps:
1) medicine juice soaks, take the crude drug of following parts by weight: Folium Mori and Herba Artemisiae Annuae each 70-100 part, join in the water of crude drug amount 16-20 times and decoct 50-70 minute, filter, repeat to decoct three times, merge three decocting liquids, decocting liquid is concentrated into 1000ml, clean Semen sojae atricolor 1000 parts is admixed in decocting liquid and soak 5.5-6.5 hour, after decocting liquid exhausts, so that beans is the most glutted；
2) steam: the Semen sojae atricolor after step 1) is soaked is placed in steamer, Steam by water bath 1-2 hour, dry moisture after taking-up, then be placed in bamboo weaving container, cover the Semen sojae atricolor surface after steaming with the Folium Mori slag decocted and Herba Artemisiae Annuae slag；
3) fermentation is concocted: by step 2) in container Semen sojae atricolor put into temperature be 28-32 DEG C, humidity be that the incubator of 60-80% carries out natural fermentation, timing is stirred, ferment 5-7 days, make, all over Planta La flavescens (Fabricius)． on Semen sojae atricolor surface, to take out, remove Folium Mori slag and Herba Artemisiae Annuae slag, wash away Planta La flavescens (Fabricius)．, being placed in ceramic vessel with cover, Folium Mori slag and Herba Artemisiae Annuae slag cover, and lid ceramic vessel lid also uses water-stop；Be subsequently placed in temperature be 28-32 DEG C, humidity be the most vexed 12-15 days of the incubator of 60-80%, to fully fermentation, take out when fragrance overflows；
4) finished product: the goods concocted through step 3) fermentation are placed in steamer, Steam by water bath 0.4-0.5 hour, dry and be Semen Sojae Preparatum finished product.
The Semen Sojae Preparatum fermentation concocting method of high enriching gamma-aminobutyric the most according to claim 1, it is characterised in that in step 3): every 3 days of the most vexed period poured out goods, fully stirred.
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110024962A (en) * | 2019-04-16 | 2019-07-19 | 河南省青山药业股份有限公司 | A kind of preparation method of Fermented Soybean |
| CN110393266A (en) * | 2018-04-24 | 2019-11-01 | 沈阳药科大学 | A kind of preparation method of the nutrition of purebred strain fermentation and functional Fermented Soybean |
| CN111388530A (en) * | 2019-11-18 | 2020-07-10 | 浙江桐君堂中药饮片有限公司 | Fermentation method of fermented soybean |
| CN111972616A (en) * | 2020-06-24 | 2020-11-24 | 江西中医药大学 | Pure-breed fermentation method of fermented soybean rich in gamma-aminobutyric acid, fermented soybean and application |
| CN112471417A (en) * | 2020-11-30 | 2021-03-12 | 国药集团冯了性（佛山）药材饮片有限公司 | Preparation method of fermented soybean and fermented soybean |
| CN112471416A (en) * | 2020-11-18 | 2021-03-12 | 四川辅正药业股份有限公司 | Preparation process of fermented soybean |
| CN115777874A (en) * | 2022-09-29 | 2023-03-14 | 中国人民解放军海军军医大学 | Fermented soybean fermentation process based on abundance and diversity of microbial flora |
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| CN101340823A (en) * | 2005-12-26 | 2009-01-07 | Cj第一制糖株式会社 | Manufacturing method for fermented soybeans having increased gamma-aminobutyric acid content |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110393266A (en) * | 2018-04-24 | 2019-11-01 | 沈阳药科大学 | A kind of preparation method of the nutrition of purebred strain fermentation and functional Fermented Soybean |
| CN110024962A (en) * | 2019-04-16 | 2019-07-19 | 河南省青山药业股份有限公司 | A kind of preparation method of Fermented Soybean |
| CN111388530A (en) * | 2019-11-18 | 2020-07-10 | 浙江桐君堂中药饮片有限公司 | Fermentation method of fermented soybean |
| CN111972616A (en) * | 2020-06-24 | 2020-11-24 | 江西中医药大学 | Pure-breed fermentation method of fermented soybean rich in gamma-aminobutyric acid, fermented soybean and application |
| CN112471416A (en) * | 2020-11-18 | 2021-03-12 | 四川辅正药业股份有限公司 | Preparation process of fermented soybean |
| CN112471417A (en) * | 2020-11-30 | 2021-03-12 | 国药集团冯了性（佛山）药材饮片有限公司 | Preparation method of fermented soybean and fermented soybean |
| CN115777874A (en) * | 2022-09-29 | 2023-03-14 | 中国人民解放军海军军医大学 | Fermented soybean fermentation process based on abundance and diversity of microbial flora |
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Application publication date: 20160720 |