CN105758827A - Protein detection fiber optic sensor based on TFBG-SPR - Google Patents
Protein detection fiber optic sensor based on TFBG-SPR Download PDFInfo
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- CN105758827A CN105758827A CN201610289282.0A CN201610289282A CN105758827A CN 105758827 A CN105758827 A CN 105758827A CN 201610289282 A CN201610289282 A CN 201610289282A CN 105758827 A CN105758827 A CN 105758827A
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- tfbg
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/59—Transmissivity
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/17—Systems in which incident light is modified in accordance with the properties of the material investigated
- G01N21/59—Transmissivity
- G01N2021/5903—Transmissivity using surface plasmon resonance [SPR], e.g. extraordinary optical transmission [EOT]
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- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
Abstract
The invention discloses a protein detection fiber optic sensor based on TFBG-SPR. The protein detection fiber optic sensor consists of a wideband light source, a polarization controller, a transmission optical fiber, a sensor, TFBG, a surface biomolecular recognizing film, and a spectrometer, wherein the wideband light source is connected with the polarization controller, and the polarization controller is connected with the left end of the sensor through the transmission optical fiber; the right end of the sensor is connected with the spectrometer through the transmission optical fiber. After a polarization state is adjusted through the polarization controller, light beams emitted by the wideband light source are incident to the sensor, and because the inner part of the sensor is carved with the TFBG, forward conductive incidence light is effectively stimulated to a cladding mold; when molecules which can interact with the surface biomolecular recognizing film exist in a sample to tested, gold film surface refractive indexes can be changed, so that SPR signals are changed; the changes of the SPR signals are detected by the spectrometer, so that protein can be detected.
Description
Technical field
The invention belongs to technical field of optical fiber sensing, be specifically related to a kind of protein detection Fibre Optical Sensor based on TFBG-SPR.
Background technology
In many fields, the detection quick and sensitive to analytes in low concentration it is critical that, such as medical domain, environment prison
The fields such as survey field, food security.The good scheme solving this problem is to use Fibre Optical Sensor, because Fibre Optical Sensor
Device has that highly sensitive, transmission information is big, security high.Fibre Optical Sensor based on inclined optical fiber grating (TFBG) can
Well to avoid using tradition to delete the method for covering to the illumination measuring in fibre core.
SPR technique the past within 10 years, have significant progress, application include film, the formation of self assembled monolayer and
Character, the reciprocation between protein, nucleosides, pharmaceuticals, surfactant equimolecular.SPR can be with real-time monitored to molecule
In conjunction with superficial phenomena such as, film formation, and high sensitivity, the signal of the most minimum high non-specific binding can be provided.
FTBG-SPR sensor, can work in a large-scale external agency refractive index so that Temperature cross-over sensitiveness is
The integrality of optical fiber structure is not the most destroyed while little.There is low cost and portable feature, and it can be with real-time collecting
On-the-spot and long-range measurement data.
Summary of the invention
For the deficiencies in the prior art, it is an object of the invention to provide a kind of protein detection Fibre Optical Sensor based on TFBG-SPR
Device, by spr signal intensity reactive protein concentration in transmitted spectrum, this structure is easily achieved, highly sensitive.
The present invention is achieved through the following technical solutions: a kind of protein detection Fibre Optical Sensor based on TFBG-SPR, by broadband light
Source (1), Polarization Controller (2), Transmission Fibers (3), sensor (4), TFBG (5), surface biomolecules identification film (6),
Spectrometer (7) forms, it is characterised in that: light source (1) is connected with Polarization Controller (2), and Polarization Controller (2) passes through
Transmission Fibers (3) is connected with sensor (4) left end, and sensor (4) right-hand member is by Transmission Fibers (3) and spectrometer (7)
It is connected.
Described sensor (4) is carved with the single-mode fiber of the standard of TFBG (5) and constitutes with a fibre core, single-mode fiber model
For healthy and free from worry SMF-28, surface uniform plated with gold coating, coating layer thickness is 50nm, divides at gold plating extenal fixation layer of surface biology
Son identifies film (6), and specifically used Thrombin aptamer, operation wavelength is at 1520nm-1570nm.
Described TFBG (5) phase mask method makes: it needs with the ultraviolet lighting of 248nm to the naked light being flushed with hydrogen gas
Fine surface, the longitudinal axis of the planar tilt sub-optical fibre of grating 10 °.
Described spectrometer (7) model is Si720.
The operation principle of the present invention is: wideband light source (1) launches the light beam of wavelength 1520nm～1570nm, is controlled by polarization
Along the incident sensor (4) of Transmission Fibers (3) after device processed (2) adjustment polarization state.Owing to being carved with TFBG (5) in sensor (4),
The incident light of forward conduction is effectively excited to cladding mode, can be with biomolecule identification film (6) phase on surface when having in determinand
The molecule of interaction, can cause gold film surface refractive index change, cause spr signal to change, detect SPR by spectrometer (7)
Signal intensity, thus detect protein.
The invention has the beneficial effects as follows: (1) uses a common standard traffic single-mode fiber (healthy and free from worry SMF-28), within it carves
There is TFBG, eliminate the impact that Temperature cross-over is sensitive, effectively simplify apparatus structure, saved cost of manufacture；(2) single-mode optics
Fine loss is little, it is possible to achieve remotely measure；(3) inclination angle of fiber grating can adjust, and the scope of measurement is wide；Therefore,
The present invention has simple in construction, sensitivity advantages of higher, it is possible to realizes the high precision test to protein, supervises online for protein
Survey provides a kind of realistic plan.
Accompanying drawing explanation
Fig. 1 is the systematic schematic diagram of a kind of protein detection Fibre Optical Sensor based on TFBG-SPR.
Fig. 2 is the cross sectional representation of sensing head in a kind of protein detection Fibre Optical Sensor based on TFBG-SPR.
Detailed description of the invention
With detailed description of the invention, the present invention is described in further detail below in conjunction with the accompanying drawings.
Seeing accompanying drawing 1, a kind of protein detection Fibre Optical Sensor based on TFBG-SPR, by wideband light source (1), polarization control
Device processed (2), Transmission Fibers (3), sensor (4), TFBG (5), surface biomolecules identification film (6), spectrometer (7)
Composition, it is characterised in that: wideband light source (1) is connected with Polarization Controller (2), and Polarization Controller (2) is by transmission light
Fine (3) are connected with sensor (4) left end, and sensor (4) right-hand member is connected with spectrometer (7) by Transmission Fibers (3)；
Sensor (4) is carved with the single-mode fiber of the standard of TFBG (5) and constitutes with a fibre core, and single-mode fiber model is healthy and free from worry SMF-28,
Surface uniform plated with gold coating, coating layer thickness is 50nm, at gold plating extenal fixation layer of surface biomolecule identification film (6),
Specifically used Thrombin aptamer, operation wavelength is at 1520nm-1570nm；TFBG (5) phase mask method makes:
It needs the surface with the ultraviolet lighting of 248nm to the bare fibre being flushed with hydrogen gas, and the planar tilt of grating is in the longitudinal axis of optical fiber
10°；Spectrometer (7) model is Si720.
The operation principle of the present invention is: wideband light source (1) launches the light beam of wavelength 1520nm～1570nm, is controlled by polarization
Along the incident sensor (4) of Transmission Fibers (3) after device processed (2) adjustment polarization state.Owing to being carved with TFBG (5) in sensor (4),
The incident light of forward conduction is effectively excited to cladding mode, when protein and biomolecule identification film (6) on surface in testing sample
Interact, gold film surface refractive index change can be caused, cause spr signal to change, believed by spectrometer (7) detection SPR
Number change, thus detects protein.
Claims (4)
1. a protein detection Fibre Optical Sensor based on TFBG-SPR, by wideband light source (1), Polarization Controller (2), transmission
Optical fiber (3), sensor (4), TFBG (5), surface biomolecules identification film (6), spectrometer (7) forms, and its feature exists
In: light source (1) is connected with Polarization Controller (2), and Polarization Controller (2) is by Transmission Fibers (3) and sensor (4)
Left end is connected, and sensor (4) right-hand member is connected with spectrometer (7) by Transmission Fibers (3).
A kind of protein detection Fibre Optical Sensor based on TFBG-SPR the most according to claim 1, it is characterised in that: described
Sensor (4) be carved with a fibre core TFBG (5) standard single-mode fiber constitute, single-mode fiber model is healthy and free from worry SMF-28,
Surface uniform plated with gold coating, coating layer thickness is 50nm, at gold plating extenal fixation layer of surface biomembrane (6), specifically used solidifying
Hemase aptamer, operation wavelength is at 1520nm-1570nm.
A kind of protein detection Fibre Optical Sensor based on TFBG-SPR the most according to claim 1, it is characterised in that: described
TFBG (5) make by phase mask method: it needs the surface with the ultraviolet lighting of 248nm to the bare fibre being flushed with hydrogen gas,
The planar tilt of grating is in the longitudinal axis 10 ° of optical fiber.
A kind of protein detection Fibre Optical Sensor based on TFBG-SPR the most according to claim 1, it is characterised in that: described
Spectrometer (7) model be Si720.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610289282.0A CN105758827A (en) | 2016-05-04 | 2016-05-04 | Protein detection fiber optic sensor based on TFBG-SPR |
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| CN201610289282.0A CN105758827A (en) | 2016-05-04 | 2016-05-04 | Protein detection fiber optic sensor based on TFBG-SPR |
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| CN201610289282.0A Pending CN105758827A (en) | 2016-05-04 | 2016-05-04 | Protein detection fiber optic sensor based on TFBG-SPR |
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Cited By (5)
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|---|---|---|---|---|
| CN106706562A (en) * | 2017-01-12 | 2017-05-24 | 天津大学 | All-optical-fiber based gas-liquid mixed measurement device and remote gas-liquid mixed measurement system |
| CN106770027A (en) * | 2016-11-15 | 2017-05-31 | 北京交通大学 | Bioprotein detection sensor-based system based on four-core fiber |
| CN108318060A (en) * | 2017-12-29 | 2018-07-24 | 北京交通大学 | Based on the 2 mu m waveband tri-consult volume fibre optical sensors for tilting optical fiber Bragg raster |
| CN108844919A (en) * | 2018-04-25 | 2018-11-20 | 暨南大学 | The reflection type inclined fiber grating index sensor of covering and production, measurement method |
| CN112345461A (en) * | 2020-11-05 | 2021-02-09 | 海南大学 | New coronavirus detection equipment and detection optical fiber preparation method thereof |
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|---|---|---|---|---|
| CN106770027A (en) * | 2016-11-15 | 2017-05-31 | 北京交通大学 | Bioprotein detection sensor-based system based on four-core fiber |
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| CN108844919A (en) * | 2018-04-25 | 2018-11-20 | 暨南大学 | The reflection type inclined fiber grating index sensor of covering and production, measurement method |
| CN108844919B (en) * | 2018-04-25 | 2020-11-13 | 暨南大学 | Cladding reflection type inclined fiber grating refractive index sensor and manufacturing and measuring methods thereof |
| CN112345461A (en) * | 2020-11-05 | 2021-02-09 | 海南大学 | New coronavirus detection equipment and detection optical fiber preparation method thereof |
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Application publication date: 20160713 |