CN112345461A - New coronavirus detection equipment and detection optical fiber preparation method thereof - Google Patents
New coronavirus detection equipment and detection optical fiber preparation method thereof Download PDFInfo
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Abstract
The invention discloses a new coronavirus detection device, which comprises: the device comprises a light source, a polarization control device, a detection optical fiber and a spectrometer; the output end of the light source is connected with the polarization control device, and the output end of the polarization control device is connected with the input end of the detection optical fiber; the output end of the detection optical fiber is connected with the input end of the spectrometer; the surface of the detection section of the detection optical fiber is provided with a metal nano-film layer, and the surface of the metal nano-film layer is provided with a new coronavirus antibody. Be provided with new coronavirus antibody on detecting optical fiber surface metal nanometer rete in this application, based on new coronavirus antibody and new coronavirus combination front and back, this principle of plasma resonance's light wave production skew takes place in metal nanometer rete surface, and the detection that can be convenient quick goes out new coronavirus, is favorable to new coronavirus carrier's quick investigation. The application also provides a preparation method of the detection optical fiber of the detection equipment for the new coronavirus, and the preparation method has the beneficial effects.
Description
Technical Field
The invention relates to the technical field of new coronavirus detection, in particular to new coronavirus detection equipment and a detection optical fiber preparation method thereof.
Background
The existing new coronavirus detection method mainly adopts throat swab nucleic acid detection, and the method needs to collect a throat swab sample of a suspected patient, then carries out nucleic acid extraction on the throat swab sample, uses a fluorescent chemical substance to determine the total product amount of nucleic acid after each Polymerase Chain Reaction (PCR) cycle through a fluorescent PCR technology, and then judges whether the nucleic acid detection is negative or positive according to the amount of the product after PCR amplification, thereby realizing the nucleic acid detection of the suspected patient and determining whether the suspected patient is infected with the new coronavirus.
Since nucleic acid detection can be performed only by professional nucleic acid detection facilities, it is necessary to send a throat swab sample to a professional nucleic acid detection facility, and a waiting time of 2 to 3 days is generally required. The suspected patient needs to be isolated during waiting for the detection result, and the suspected patient cannot be screened quickly and accurately.
Disclosure of Invention
The invention aims to provide new coronavirus detection equipment and a preparation method of a detection optical fiber of the new coronavirus detection equipment, which are beneficial to simply and quickly detecting new coronavirus in a throat swab, reducing the detection difficulty of the new coronavirus and improving the detection efficiency.
In order to solve the above technical problem, the present invention provides a new coronavirus detection apparatus, comprising:
the device comprises a light source, a polarization control device, a detection optical fiber and a spectrometer; the output end of the light source is connected with the input end of the polarization control device, and the output end of the polarization control device is connected with the input end of the detection optical fiber; the output end of the detection optical fiber is connected with the input end of the spectrometer;
the polarization control device is used for converting light rays output by the light source into P polarized light and inputting the P polarized light to the detection optical fiber;
the surface of the detection section of the detection optical fiber is provided with a metal nano-film layer which generates plasma resonance on incident light, the surface of the metal nano-film layer is provided with a new coronavirus antibody, and when the new coronavirus antibody and the new coronavirus are specifically combined, the light wave band of the surface of the metal nano-film layer which generates plasma resonance is shifted;
the spectrometer is used for performing spectrum detection on the light output from the detection optical fiber to determine whether the light wave band in which the plasma resonance occurs shifts.
In an optional embodiment of the present application, a core layer of the detection section of the detection optical fiber is further provided with a slanted bragg grating.
In an optional embodiment of the present application, the slanted bragg grating has an inclination angle of 5 to 15 degrees with respect to a lengthwise direction of the detection fiber.
In an optional embodiment of the present application, the thickness of the metal nano-film layer is 45nm to 55 nm.
In an alternative embodiment of the present application, the light source is adapted to output light in the wavelength range 1400nm-1750 nm.
The application also provides a preparation method of the detection optical fiber of the new coronavirus detection equipment, which comprises the following steps:
carrying out sensitization treatment on a single mode fiber, and plating a metal nano film layer on the surface of the sensitized single mode fiber;
carrying out plasma modification on the single-mode optical fiber in isopropanol fluid to enable the surface of the metal nano film layer to have hydroxyl;
washing the single-mode optical fiber by phosphate buffer solution, so that the carboxyl is converted into an active functional group;
and placing the single-mode optical fiber in a new coronavirus antibody buffer solution to enable the active functional group to be combined with the new coronavirus antibody, so as to obtain the detection optical fiber carrying the new coronavirus antibody.
In an optional embodiment of the present application, after performing the sensitization process on the single-mode optical fiber, the method further includes:
and etching the inclined Bragg grating on the single-mode optical fiber by adopting a pulse laser beam through a mask plate, and then performing the operation of plating a metal nano film layer on the surface of the single-mode optical fiber after the sensitization treatment.
In an optional embodiment of the present application, placing the single mode optical fiber in a new coronavirus antibody buffer comprises:
and placing the single-mode optical fiber in a new coronavirus antibody buffer solution with the flow rate of 35 ul/min-50 ul/min and the temperature of 22-30 ℃ for at least 1 h.
The invention provides a new coronavirus detection device, which comprises: the device comprises a light source, a polarization control device, a detection optical fiber and a spectrometer; the output end of the light source is connected with the input end of the polarization control device, and the output end of the polarization control device is connected with the input end of the detection optical fiber; the output end of the detection optical fiber is connected with the input end of the spectrometer; the polarization control device is used for converting light rays output by the light source into P polarized light and inputting the P polarized light to the detection optical fiber; the surface of the detection section of the detection optical fiber is provided with a metal nano-film layer which generates plasma resonance on incident light, the surface of the metal nano-film layer is provided with a new coronavirus antibody, and when the new coronavirus antibody and the new coronavirus are specifically combined, the light wave band of the surface of the metal nano-film layer which generates plasma resonance shifts; the spectrometer is used for performing spectrum detection on light output from the detection optical fiber to determine whether the light wave band in which the plasma resonance occurs is shifted.
The new coronavirus detection equipment that provides in this application is detecting the detection area section surface on the optic fibre and is setting up metal nanometer rete, when light transmission set up this metal nanometer rete to detecting optic fibre detection area section surface, the light wave of specific wave band can take place plasma resonance on metal nanometer rete surface. Meanwhile, a new coronavirus antibody is arranged on the surface of the metal nano-film layer; if the new coronavirus antibody and the new coronavirus are specifically bound, that is, the film on the outer surface of the detection section is changed from a film in which the metal nano-film and the new coronavirus antibody are bound to each other into a film in which the metal nano-film and the new coronavirus antibody are specifically bound to each other, the refractive index of the film on the outer surface of the detection optical fiber is changed correspondingly to the change of the material of the film. When the light in the detection optical fiber is transmitted to the surface of the metal nanometer film layer, the light wave band of the plasma resonance can be shifted.
Therefore, before and after the specific combination of the new coronavirus antibody on the surface of the detection optical fiber and the new coronavirus is carried out, the plasma resonance waveband can be shifted according to the principle that the detection section of the detection optical fiber is in contact with the pharyngeal swab suspected of a patient, light rays incident to the detection optical fiber are provided through the light source and the polarization control device, the spectrometer detects the light spectrum output from the detection optical fiber, the light wave waveband of the plasma resonance is identified based on the light spectrum, whether the new coronavirus exists in the pharyngeal swab suspected of the patient is confirmed, and the detection of the new coronavirus of the suspected patient is rapidly realized. The method is simple and effective, can conveniently and rapidly detect the new coronavirus, and is beneficial to rapidly examining new coronavirus carriers.
The application also provides a preparation method of the detection optical fiber of the detection equipment for the new coronavirus, and the preparation method has the beneficial effects.
Drawings
In order to more clearly illustrate the embodiments or technical solutions of the present invention, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a novel coronavirus detection device provided in an embodiment of the present application;
fig. 2 is a schematic flow chart of a method for manufacturing a detection optical fiber according to an embodiment of the present application.
Detailed Description
The new coronavirus is also called as novel coronavirus, which is a novel coronavirus which is mainly developed in early 2020 at present and is named as COVID-19 in English. Because there is a certain asymptomatic latent period after the virus is infected, but the infectivity is very strong, so the investigation of people at risk of infection is an important measure for inhibiting the virus infection.
At present, the investigation of the new coronavirus mainly depends on nucleic acid detection, needs professional institutions to perform the investigation, and generally needs two to three days to obtain a detection result. This undoubtedly brings about difficulties in the nucleic acid detection work.
Therefore, the technical scheme capable of rapidly detecting the new coronavirus on site is provided.
In order that those skilled in the art will better understand the disclosure, the invention will be described in further detail with reference to the accompanying drawings and specific embodiments. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
As shown in fig. 1, fig. 1 is a schematic structural diagram of a new coronavirus detection device provided in an embodiment of the present application, and the device may include:
the device comprises a light source 1, a polarization control device 2, a detection optical fiber 3 and a spectrometer 6;
the output end of the light source 1 is connected with the input end of the polarization control device 2;
the output end of the polarization control device 2 is connected with the input end of the detection optical fiber 3;
the output end of the detection optical fiber 3 is connected with the input end of the spectrometer 6;
the polarization control device 2 is used for converting light output by the light source 1 into P-polarized light and inputting the P-polarized light into the detection optical fiber 3;
the surface of the detection section of the detection optical fiber 3 is provided with a metal nano-film layer 5 which generates plasma resonance to incident light, the surface of the metal nano-film layer 5 is provided with a new coronavirus antibody, and when the new coronavirus antibody and the new coronavirus are specifically combined, the light wave band of the metal nano-film layer 5 which generates plasma resonance shifts;
the spectrometer 6 is used for performing spectrum detection on the light output from the detection fiber 3 to determine whether the light band in which the plasmon resonance occurs is shifted.
It should be noted that, the metal nano-film layer 5 is disposed on the surface of the detection section on the detection optical fiber 3, and when light is transmitted to the detection section on which the detection optical fiber 3 is disposed on the metal nano-film layer 5, light waves of a specific waveband can generate plasma resonance on the surface of the metal nano-film layer 5. The surface of the detection optical fiber 3 is provided with a metal nano-film layer 5, and the surface of the metal nano-film layer 5 is provided with a new coronavirus antibody, which is equivalent to forming an SPR (surface plasma resonance) sensor, and the detection of the new coronavirus is realized by utilizing the plasma resonance technology of the metal nano-film layer 5.
When detecting the new coronavirus, a pharyngeal swab, blood or other body fluid possibly containing the new coronavirus of a person to be detected can be taken as liquid to be detected, the liquid to be detected is attached to the surface of the detection section of the detection optical fiber 3, or the detection section is immersed in the liquid to be detected, and the specific operation mode is determined according to the amount of the liquid to be detected, so that the proper temperature and humidity are kept.
If new coronavirus exists in the liquid to be detected, the liquid to be detected can react with the new coronavirus antibody on the surface of the metal nano-film layer 5 in the detection section of the detection optical fiber 3, and the substance attached to the surface of the metal nano-film layer 5 is not the new coronavirus antibody any more but a combination of the new coronavirus antibody and the new coronavirus. Because the substance attached to the surface of the metal nano-film layer 5 changes, which is equivalent to that the refractive index of the film layer attached to the outer surface of the detection section by the detection optical fiber 3 changes, when light passes through the detection section by the detection optical fiber 3, the light wave band capable of generating plasma resonance changes correspondingly, and the resonance wave band also shifts, so that whether the liquid to be detected contains the new corona virus can be detected according to the principle that the light wave band capable of generating plasma resonance is different before and after the new corona virus antibody and the new corona virus on the surface of the metal nano-film layer 3 of the detection section are combined.
In this embodiment, the light source 1 may be a broad-spectrum laser light source, and the light output by the light source may be in the range of 1400nm to 1750 nm.
Light output from the light source 1 is passed through the polarization control device 2 for generating polarized light in the p-polarization state to excite SPR resonance. Polarized light enters the detection optical fiber 3 and is totally reflected and conducted inside the detection optical fiber 3, and when the light is conducted to a detection section, plasma resonance occurs in the detection section due to the fact that the metal nano film layer 5 is arranged on the surface of the detection section. The thickness of the metal nano-film layer 5 may be set at 45nm to 55 nm.
Optionally, in the detection section of the detection optical fiber 3, an inclined bragg grating 4 may be further disposed, where the inclined bragg grating 4 is disposed in a core layer of the detection section of the detection optical fiber 3, and light is excited in the inclined bragg grating 4 due to the periodic change of the refractive index, so as to generate a large number of cladding modes, which is beneficial to improve the accuracy of the detection result, and an inclination angle of the inclined bragg grating 4 with respect to the length direction of the detection optical fiber 3 is 5 degrees to 15 degrees.
When the light is totally reflected on the surface of the metal nano-film layer 5, the light can be transmitted in the form of evanescent waves, and plasma waves existing in the metal nano-film layer 5 can form resonance with the evanescent waves, so that the intensity of the reflected light is greatly attenuated.
The SPR high sensitivity is characterized in that the excitation mode of plasma waves is influenced remarkably by the surface condition of the metal nano-film layer 5, and after the new coronavirus antibody on the surface of the metal nano-film layer 5 is specifically combined with the new coronavirus in the pharyngeal swab of a person to be detected through specific combination, the resonance waves in a spectrometer are shifted due to the weak changes of the refractive index and the like. Based on the analysis of the spectrometer on the transmission spectrum, an analysis result can be obtained by the shift of the SPR resonance wavelength of the spectrometer after 10 minutes, and whether the liquid to be detected contains the new coronavirus can be determined.
The new coronavirus detection device provided by the application can be combined with a new coronavirus antibody by using the new coronavirus, and the refractive index of the outer surface film layer of the detection optical fiber 3 is changed according to the principle, so that the new coronavirus is rapidly detected. Detection equipment principle simple structure in this application need not to deliver to professional detection mechanism with the pharynx swab, can carry out quick analysis to body fluids such as await measuring personnel's pharynx swab promptly and detect, confirms whether the await measuring personnel infects new coronavirus, the detection degree of difficulty of the new coronavirus of reduction that can be very big improves the detection efficiency of new coronavirus.
The application also provides an embodiment of a preparation method of the detection optical fiber in the detection equipment for preparing the new coronavirus. As shown in fig. 2, fig. 2 is a schematic flow chart of a method for manufacturing a detection optical fiber according to an embodiment of the present application, where the method for manufacturing a detection optical fiber includes:
s1: carrying out sensitization treatment on the single-mode optical fiber, and plating a metal nano film layer on the surface of the sensitized single-mode optical fiber.
A single-mode fiber with the tail fiber of about 1m can be selected, the single-mode fiber is placed in a hydrogen environment for sensitization, the environment temperature can be about 60 ℃ in the sensitization process, the hydrogen gas pressure can be 1500-1600psi, and the sensitization time is about one week.
After the single-mode fiber is subjected to sensitization treatment, a metal nano-film layer can be arranged on the surface of the single-mode fiber, and the thickness of the metal nano-film layer can be 45nm to 55 nm.
Of course, in order to excite a large number of cladding modes of the detected optical fiber and improve the accuracy of the detection result of the new coronavirus, the inclined bragg grating may be etched on the single-mode optical fiber after the sensitization process.
For example, an excimer laser of about 193nm is used to focus a pulse laser beam on a phase mask by condensing the laser beam with a mirror and a focusing lens, and the mask is rotated by 5 ° to 15 ° with respect to a single-mode fiber, whereby an inclined bragg grating having an inclination angle of 5 ° to 15 ° and a grating region length of about 1cm can be written.
After the inclined Bragg grating is etched, a metal nano film layer is plated on the surface of the single-mode optical fiber.
S2: the single-mode fiber is subjected to plasma modification in isopropanol fluid, so that the surface of the metal nano film layer is provided with hydroxyl.
In the process of carrying out plasma modification on the single-mode optical fiber, plasma modification is carried out in isopropanol fluid for about 15 minutes, so that the surface of the metal nano-film layer can have hydroxyl groups.
S3: the single mode fiber was washed with phosphate buffer and N-hydroxysuccinimide (NHS) and 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride were passed through to convert the carboxyl group to an active functional group.
After being washed by phosphate buffer solution, N-hydroxysuccinimide (NHS) and 1- (3-dimethylaminopropyl) -3-ethylcarbodiimide hydrochloride which can be mixed according to a certain proportion can convert carboxyl on the surface of the metal nano-film layer into an active functional group so as to be combined with a new coronavirus antibody.
S4: and placing the single-mode optical fiber in a buffer solution of the new coronavirus antibody to combine the active functional group with the new coronavirus antibody to obtain the detection optical fiber carrying the new coronavirus antibody.
The single mode fiber is placed in the buffer solution of the new coronavirus antibody, the flow rate of the buffer solution of the new coronavirus antibody can be controlled to be 35 ul/min-50 ul/min, the temperature is constant at 22-30 ℃, and the optimal temperature is 26 ℃. After at least 1h, the detection optical fiber is prepared by combining the active functional group with the surface chemical bond of the new coronavirus antibody.
The detection optical fiber is connected with devices such as a light source, a polarization control device, a spectrometer and the like, so that the detection equipment of the new coronavirus can be formed. Simple structure, low production cost and is beneficial to the quick investigation of suspected patients of new coronavirus.
It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Furthermore, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include elements inherent in the list. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element. In addition, parts of the above technical solutions provided in the embodiments of the present application, which are consistent with the implementation principles of corresponding technical solutions in the prior art, are not described in detail so as to avoid redundant description.
The principles and embodiments of the present invention are explained herein using specific examples, which are presented only to assist in understanding the method and its core concepts. It should be noted that, for those skilled in the art, it is possible to make various improvements and modifications to the present invention without departing from the principle of the present invention, and those improvements and modifications also fall within the scope of the claims of the present invention.
Claims (8)
1. A new coronavirus detection device, comprising: the device comprises a light source, a polarization control device, a detection optical fiber and a spectrometer; the output end of the light source is connected with the input end of the polarization control device, and the output end of the polarization control device is connected with the input end of the detection optical fiber; the output end of the detection optical fiber is connected with the input end of the spectrometer;
the polarization control device is used for converting light rays output by the light source into P polarized light and inputting the P polarized light to the detection optical fiber;
the surface of the detection section of the detection optical fiber is provided with a metal nano-film layer which generates plasma resonance on incident light, the surface of the metal nano-film layer is provided with a new coronavirus antibody, and when the new coronavirus antibody and the new coronavirus are specifically combined, the light wave band of the surface of the metal nano-film layer which generates plasma resonance is shifted;
the spectrometer is used for performing spectrum detection on the light output from the detection optical fiber to determine whether the light wave band in which the plasma resonance occurs shifts.
2. The new coronavirus detection device of claim 1, wherein a core layer of the detection section of the detection optical fiber is further provided with a tilted bragg grating.
3. The apparatus for detecting a new coronavirus according to claim 1, wherein the tilted bragg grating is tilted at an angle of 5 to 15 degrees with respect to the lengthwise direction of the detection optical fiber.
4. The apparatus for detecting a novel coronavirus according to claim 1, wherein the thickness of the metal nanolayer film is 45nm to 55 nm.
5. The apparatus for detecting a new coronavirus according to claim 1, wherein the light source is configured to output light in a wavelength range of 1400nm to 1750 nm.
6. A method for preparing a detection optical fiber of new coronavirus detection equipment is characterized by comprising the following steps:
carrying out sensitization treatment on a single mode fiber, and plating a metal nano film layer on the surface of the sensitized single mode fiber;
carrying out plasma modification on the single-mode optical fiber in isopropanol fluid to enable the surface of the metal nano film layer to have hydroxyl;
washing the single-mode optical fiber by phosphate buffer solution, so that the carboxyl is converted into an active functional group;
and placing the single-mode optical fiber in a new coronavirus antibody buffer solution to enable the active functional group to be combined with the new coronavirus antibody, so as to obtain the detection optical fiber carrying the new coronavirus antibody.
7. The method for preparing a detection optical fiber of a new coronavirus detection device according to claim 6, further comprising, after the sensitizing the single mode optical fiber:
and etching the inclined Bragg grating on the single-mode optical fiber by adopting a pulse laser beam through a mask plate, and then performing the operation of plating a metal nano film layer on the surface of the single-mode optical fiber after the sensitization treatment.
8. The method for preparing the detection optical fiber of the new coronavirus detection device of claim 6, wherein the step of placing the single-mode optical fiber in the new coronavirus antibody buffer comprises the following steps:
and placing the single-mode optical fiber in the new coronavirus antibody buffer solution with the flow rate of 35 ul/min-50 ul/min and the temperature of 22-30 ℃ for at least 1 h.
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