CN105753572A - Microbial organic master batch and preparation method thereof - Google Patents

Abstract

The invention provides a microbial organic master batch. The microbial organic master batch is prepared from the following main components in percentage by weight: 65%-85% of industrial waste, 0-10% of an industrial alkali raw material, 0-20% of an agricultural fertilizer, 1%-10% of an active agent, 0.1%-10% of a microbial agent and 2%-5% of a liquid aid. The invention further provides a preparation method of the microbial organic master batch. The preparation method comprises the following steps: (1) adding the industrial waste into a container, stirring, and adding the liquid aid; (2) continuously adding the industrial alkali raw material, the agricultural fertilizer, the active agent and the microbial agent, and stirring for 3-5 minutes; (3) adding the liquid aid, and continuing to stir for 2-3 minutes; and (4) drying, and sieving.

Description

A kind of organic master batch of microorganism and preparation method thereof

Technical field

The present invention relates to chemical field, in particular to a kind of organic master batch of microorganism and Its preparation method.

Background technology

Microbial manure is increasingly weighed by national and agricultural technology extension department and user Depending on, also it is hope and the only way which must be passed of fertilizer needed for agricultural sustainable development from now on, commonly side Method produces microbial manure and what strange thing use is not the most, but, utilize under industry It is (micro-that heel and discarded object and microbial bacterial agent combine a kind of novel microbial fertilizer of coupling production Biological organic master batch) the most do not met, the present invention open microorganism field and The beginning of trade waste recycling.Both met the needs of agricultural fertilizer material, solved again Industrial waste and discarded object stack the difficulty of storage nowhere, solve environmental pollution etc. many Problem, serves the effect achieved many things at one stroke, get twice the result with half the effort, and is to meet industrial and agricultural development strategy Require, be country widely popularize, the novel microbial fertilizer of market in urgent need.

In view of this, the special proposition present invention.

Summary of the invention

The first object of the present invention is to provide a kind of organic master batch of microorganism, described microorganism Organic master batch produces by utilizing industrial waste and discarded object and microbial bacterial agent to combine coupling, Both meet the needs of agricultural fertilizer material, solve again industrial waste and discarded object heap nowhere Put the difficulty of storage.

The second object of the present invention is to provide the preparation side of a kind of organic master batch of described microorganism Method, various components are effectively mixed by the method by rational technique, and technical maturity is stable, Favorable reproducibility.

In order to realize the above-mentioned purpose of the present invention, spy by the following technical solutions:

One aspect of the present invention relates to a kind of organic master batch of microorganism, and described microorganism is organic The following components of master batch main by weight percentages is made:

Trade waste: 65-85%；Industrial alkali raw material: 0-10%；Agricultural fertilizer: 0-20%； Activating agent: 1-10%；Microbial bacterial agent: 0.1-10%；Liquid adjuvants 2-5%.

Preferably, the organic master batch of described microorganism is mainly by following group by weight percentage Divide and make:

Trade waste: 70-75%；Industrial alkali raw material: 6-9%；Agricultural fertilizer: 5-10%； Activating agent: 1-5%；Microbial bacterial agent: 0.1-2%；Liquid adjuvants 3-4.5%.

Leftover bits and pieces composition mainly contains various amino acid, and pH value is between 2.8-5.5, to farming Thing injury is the biggest, it is impossible to being used directly to do fertilizer, it is permissible that the present invention adds industrial alkali raw material Regulation leftover bits and pieces pH value, can control pH value regulation according to consumption and chemical reaction thereof In the range of 5.5-8.0, do not injure crops, beneficially crops during use and absorb this microorganism Nutrient, micro-and each function bacterium in fertilizer play a role, the beneficially length of function bacterium Phase deposits and survives.

According to the pH value of different soils, industrial alkali used in the present invention and agricultural fertilizer Can on-demand add, when soil acidity or alkalinity is suitable, it might even be possible to without.

Organic nitrogen in leftover bits and pieces, phosphorus, potassium nutrition content are the highest, and adding agricultural fertilizer can adjust Joint nutrient content, supplements because adding liquid adjuvants, industrial alkali raw material, activating agent, micro-life The nutrient that thing microbial inoculum causes reduces, thus the various nutrients needed for ensureing plant growth, strengthen Plant cold resistance, disease-resistant, lodging tolerance, strengthen fertilizer efficiency.

Because the various amino acid of leftover bits and pieces have the strongest injury effect to microorganism live bacteria body, mend Filling addition agricultural fertilizer, particularly adding nitrogen element has injury effect to microorganism live bacteria body, Add activating agent, by urea in activating agent and amino acid whose chemical reaction and liquid adjuvants and The chelatropic reaction of activating agent generates the product being conducive to viable bacteria body to rely on, survive, and strengthens viable bacteria The mobility of body, it is ensured that the long-time healthy survival of viable bacteria body, increases the effect of microorganism, Make the insoluble trace element of the water in activating agent become water-soluble trace unit by chelatropic reaction simultaneously Element, for the plant a large amount of absorptions to trace element, thus adds fertilizer efficiency.

The present invention adds microbial bacterial agent, is competed by microorganism, grow, breeds and is occupied The mode of living space stops the growth of phytopathogen, can quickly form at plant surface One floor height density diaphragm, makes phytopathogen can not get living space, thus protects and plant Thing endangers from pathogen, and meanwhile, each functional microorganism thalline can secrete antibacterial substance, presses down Pathogen spore-germination processed and mycelial growth, thus reach the purpose of prevention and treatment pest and disease damage, The most also can phosphorus decomposing, potassium decomposing activating soil nutrient, produce various plants grow required Hormone, keeps soil from packing together.

Preferably, under described trade waste is Gourmet Powder Factory's particle leftover bits and pieces, yeast factory particle One or more in heel and sugar refinery particle leftover bits and pieces, it is preferable that described trade waste It it is industry Gourmet Powder Factory particle leftover bits and pieces.

Preferably, described industrial alkali raw material is industrial monohydrate, sodium acid carbonate, carbonic acid One or more in potassium, wood shavings alkali, potassium hydroxide and NaOH, it is preferable that described Industrial alkali raw material is sodium carbonate or potassium carbonate.

Preferably, described agricultural fertilizer be urea, ammonium sulfate, ammonium hydrogen carbonate, MAP, One or more in Diammonium phosphate (DAP), potassium dihydrogen phosphate, potassium sulfate and potassium chloride, it is preferable that Described agricultural fertilizer is one or more in urea, MAP, potassium sulfate.

Preferably, described activating agent is ferroso-ferric oxide, di-iron trioxide, carbon black, oxidation One in magnesium and copper sulphate, it is preferable that described activating agent is ferroso-ferric oxide or three oxidations Two iron.

Preferably, described microbial bacterial agent be bacillus subtilis, bacillus amyloliquefaciens, One or more in bacillus megaterium, bacillus licheniformis and colloid bacillus cereus, excellent Selection of land, described microbial bacterial agent is bacillus subtilis, bacillus amyloliquefaciens, colloid bud The bacterium mixing of spore bacillus or bacillus subtilis, bacillus amyloliquefaciens and colloid gemma bar Thing.

Preferably, described liquid adjuvants is urea saturated aqueous solution, fusing urea, molten sulfur Sodium thiosulfate, melted metasilicate pentahydrate sodium, melted non-hydrate sodium metasilicate and polyacrylamide aqueous amine One or more in solution, it is preferable that described liquid adjuvants is urea saturated aqueous solution.

Another aspect of the present invention relates to the preparation method of the organic master batch of described microorganism, described Method comprises the following steps:

1) trade waste is added in container, add partially liq auxiliary agent under agitation；

2) industrial alkali raw material, agricultural fertilizer, activating agent, microbial bacteria it are continuously added to successively Agent, stirs 3-5 minute after adding；3) add remaining liq auxiliary agent, continue stirring 2-3 and divide Clock；4) it is dried and sieves；Wherein, described step 1) in add liquid adjuvants with described Step 3) the middle liquid adjuvants added, volume ratio is 3-5:1.

Industrial waste by after surface spraying liquid adjuvants, and liquid adjuvants carry out special Chemical reaction produces a kind of pasty state mucus, utilize the pasty state mucus that produces by industrial alkali raw material, Agricultural fertilizer, activating agent, solid, powdery microbial inoculum are bonded on particle leftover bits and pieces surface firmly, And form thin layer smooth film.This film is firmly bonded, does not falls off, smooth surface, flowing Property is good, be prone to machine sowing and various mode is applied fertilizer.

Preferably, described step 1) in the liquid adjuvants and the described step 3 that add) in add Liquid adjuvants, volume ratio is 3-5:1.

Preferably, described step 4) in dry be under normal temperature and pressure air-dry；Described sieve Divide and described master batch is sized to particle diameter less than 4mm.

Compared with prior art, the invention have the benefit that

(1) the organic master batch of the microorganism of the present invention utilizes industrial waste and discarded object and micro-life Thing microbial inoculum combines coupling and produces, and had both met the needs of agricultural fertilizer material, and had solved again industry Leftover bits and pieces and discarded object stack the difficulty of storage nowhere；

(2) Micro-biological Fertilizer on Plant of the present invention can be administered alone, meanwhile, and again can conduct Basis master batch is used in mixed way with fertilizer, compound organic and inorganic fertilizer, Chemical Mixed Fertilizer etc., Zeng Qiangfei Effect, pre-preventing disease and pest occur；

(3) preparation method of the present invention is particularly suitable for preparing the organic master batch of described microorganism, work Skill is mature and stable, operates relatively simple, and favorable reproducibility is safe and reliable.

Accompanying drawing explanation

In order to be illustrated more clearly that the embodiment of the present invention or technical scheme of the prior art, with Lower the accompanying drawing used required in embodiment or description of the prior art will be briefly described.

Fig. 1 is the flow sheet of the organic master batch of microorganism of the present invention.

Detailed description of the invention

Below in conjunction with embodiment, embodiment of the present invention are described in detail, but this Skilled person is it will be appreciated that the following example is merely to illustrate the present invention, and should not regard For limiting the scope of the present invention.Unreceipted actual conditions person in embodiment, according to normal condition Or the condition of manufacturer's suggestion is carried out.Agents useful for same or instrument unreceipted production firm person, all For the conventional products that can be obtained by commercially available purchase.

Embodiment 1

The organic master batch of described microorganism is prepared according to following steps

1. weigh 300 kilograms of monosodium glutamate leftover bits and pieces, account for gross weight 70.26% and put metering storage bin hopper into Stand-by.

2. weigh 35 kilograms of postindustrial sodium carbonate of pulverizing, account for gross weight 8.20% and put metering into Storage bin hopper is stand-by.

3. weigh 30 kilograms of urea, MAP 10 kilograms, potassium sulfate 20 kilograms after pulverizing Mixture, account for gross weight 14.05% put into metering storage bin hopper stand-by.

4. weigh ferroso-ferric oxide 10 kilograms, account for gross weight 2.34% put into metering storage bin hopper treat With.

5. weigh 100,000,000,000 bacillus subtilises, bacillus amyloliquefaciens, colloid bacillus cereus Or the bacterium mixture 3 of bacillus subtilis, bacillus amyloliquefaciens and colloid gemma bar is public Jin, account for gross weight 0.7% put into metering storage bin hopper stand-by.

6. weigh urea saturated solution 19 kilograms, account for gross weight 4.45% and put metering storage tank into Stand-by.

Add up to: 427 kilograms

First put 300 kilograms of leftover bits and pieces into mixer, to mixed in the case of being stirred continuously Mix material continuous uniform in machine to add saturated aqueous solution of urea 15.2 kilograms and (account for total saturated solution 80%), saturated with urea the most molten in order after monosodium glutamate leftover bits and pieces surface spraying is moistening uniformly Liquid adds 35 kilograms of sodium carbonate, 60 kilograms of urea, MAP, sulphur together in mixer Acid potassium mixture, 10 kilograms of ferroso-ferric oxides, 3 kilograms of bacillus subtilises, after adding again Puddling spraying parcel 4 minutes, material will remain 20% in metering storage tank after mixing again Urea saturated solution 3.8 kilograms is added in mixer on material, then will be mixed after puddling 2 minutes Mix uniform material to be put in storage bin hopper, then material conveyer is delivered to low temperature and do Dry device carries out low temperature drying, is transported to screening machine after drying and sieves, finished product dress after screening Bag, check the weight, package, put in storage.Residue on sieve waste material treatment in situ.

Embodiment 2

The organic master batch of described microorganism is prepared according to following steps

1. weigh 450 kilograms of monosodium glutamate leftover bits and pieces, account for gross weight 80.79% and put metering storage bin hopper into Stand-by.

2. weigh 10 kilograms of postindustrial potassium carbonate of pulverizing, account for gross weight 1.80% and put metering into Storage bin hopper is stand-by.

3. weigh 15 kilograms of urea after pulverizing, account for gross weight 2.69% put into metering storage bin hopper treat With.

4. weigh di-iron trioxide 50 kilograms, account for gross weight 8.98% put into metering storage bin hopper treat With.

5. weigh 100,000,000,000 bacillus subtilis 20 kilograms, account for gross weight 3.59% and put meter into Amount storage bin hopper is stand-by.

6. weigh urea saturated solution 12 kilograms, account for gross weight 2.15% and put metering storage tank into Stand-by.

Add up to: 557 kilograms

First put 450 kilograms of leftover bits and pieces into mixer, to mixed in the case of being stirred continuously Mix material continuous uniform in machine to add saturated aqueous solution of urea 9.6 kilograms and (account for total saturated solution 80%), add in mixer continuously in order after monosodium glutamate leftover bits and pieces surface spraying is moistening uniformly 10 kilograms of potassium carbonate, 15 kilograms of urea, 50 kilograms of di-iron trioxides, 20 kilograms of withered grass buds Spore bacillus, puddles spraying parcel 4 minutes again after adding, material again will metering after mixing Remain 20% urea saturated solution 2.4 kilograms in storage tank to be added in mixer on material, then Uniform material will be puddled after puddling 2.5 minutes be put in storage bin hopper, then by material Deliver to low temperature drier with conveyer and carry out low temperature drying, be transported to screening machine after drying and carry out Screening, after screening, finished product packs, checks the weight, package, puts in storage.Residue on sieve waste material is on the spot Process.

Embodiment 3

The organic master batch of described microorganism is prepared according to following steps

1. weigh 85 kilograms of monosodium glutamate leftover bits and pieces, account for gross weight 85% put into metering storage bin hopper stand-by.

2. weigh carbon black 1 kilogram, account for gross weight 1% put into metering storage bin hopper stand-by.

3. weigh bacillus megaterium 10 kilograms, account for gross weight 10% put into metering storage bin hopper treat With.

4. weigh urea saturated solution 4 kilograms, account for gross weight 4% put into metering storage tank stand-by.

Add up to: 100 kilograms

First put leftover bits and pieces into mixer, in the case of being stirred continuously in mixer thing Material continuous uniform adds saturated aqueous solution of urea 3 kilograms (accounting for the 75% of total saturated solution), treats taste Essence leftover bits and pieces surface spraying is pressed suitable in order continuously together with urea saturated solution after moistening uniformly Order continuous adds 1 kilogram of carbon black in mixer, 10 kilograms of bacillus megateriums, after adding again Puddling spraying parcel 4 minutes, material will remain 25% in metering storage tank after mixing again Urea saturated solution 1 kilogram is added in mixer on material, then will puddle after puddling 2 minutes Uniform material is put in storage bin hopper, then material conveyer is delivered to low temperature drying Device carries out low temperature drying, is transported to screening machine after drying and sieves, finished product pack after screening, Check the weight, package, put in storage.Residue on sieve waste material treatment in situ.

Embodiment 4

The organic master batch of described microorganism is prepared according to following steps

1. weigh 70 kilograms of monosodium glutamate leftover bits and pieces, account for gross weight 70% put into metering storage bin hopper stand-by.

2. weigh 1 kilogram of postindustrial sodium carbonate of pulverizing, account for gross weight 1% and put metering storing into Struggle against stand-by.

3. after weighing pulverizing, 2 kilograms of urea, MAP 1 kilogram, potassium sulfate 1 kilogram mix Compound, account for gross weight 4% put into metering storage bin hopper stand-by.

4. weigh di-iron trioxide 10 kilograms, account for gross weight 10% put into metering storage bin hopper stand-by.

5. weigh bacillus licheniformis 10 kilograms, account for gross weight 10% put into metering storage bin hopper treat With.

6. weigh urea saturated solution 5 kilograms, account for gross weight 5% put into metering storage tank stand-by.

Add up to: 100 kilograms

First put leftover bits and pieces into mixer, in the case of being stirred continuously in mixer thing Material continuous uniform adds saturated aqueous solution of urea 4 kilograms (accounting for the 80% of total saturated solution), treats taste Essence leftover bits and pieces surface spraying is pressed upper in order continuously together with urea saturated solution after moistening uniformly State weight in mixer, add sodium carbonate, urea, MAP, potassium sulfate mixture, three Aoxidize two iron and bacillus amyloliquefaciens, puddle spraying parcel after adding again 5 minutes, thing Material is added to mixer by remaining 20% urea saturated solution in metering storage tank after mixing again In on material, more uniform material will be puddled after puddling 3 minutes and be put in storage bin hopper, Then material conveyer is delivered to low temperature drier and carry out low temperature drying, be transported to after drying Screening machine sieves, and after screening, finished product packs, checks the weight, package, puts in storage.Tail over Thing waste material treatment in situ.

Embodiment 5

The organic master batch of described microorganism is prepared according to following steps

1. weigh 65 kilograms of monosodium glutamate leftover bits and pieces, account for gross weight 65% put into metering storage bin hopper stand-by.

2. weigh 10 kilograms of postindustrial sodium carbonate of pulverizing, account for gross weight 10% and put metering storage into Hopper is stand-by.

3. after weighing pulverizing, 15 kilograms of urea, MAP 2 kilograms, potassium sulfate 3 kilograms mix Compound, account for gross weight 20% put into metering storage bin hopper stand-by.

4. weigh 5 kilograms of copper sulphate, account for gross weight 5% put into metering storage bin hopper stand-by.

5. weigh bacillus subtilis, bacillus amyloliquefaciens, colloid bacillus cereus or withered grass 8 kilograms of the bacterium mixture of bacillus, bacillus amyloliquefaciens and colloid gemma bar, accounts for It is stand-by that gross weight 8% puts metering storage bin hopper into.

6. weigh urea saturated solution 2 kilograms, account for gross weight 2% put into metering storage tank stand-by.

Add up to: 100 kilograms

First put leftover bits and pieces into mixer, in the case of being stirred continuously in mixer thing Material continuous uniform adds saturated aqueous solution of urea 1.6 kilograms (accounting for the 80% of total saturated solution), treats Monosodium glutamate leftover bits and pieces surface spraying is pressed after moistening uniformly in order continuously together with urea saturated solution Above-mentioned weight adds sodium carbonate, urea, MAP, potassium sulfate mixture in mixer, Copper sulphate and bacillus subtilis, bacillus amyloliquefaciens, colloid bacillus cereus or withered grass Bacillus, bacillus amyloliquefaciens and the bacterium mixture of colloid gemma bar, after adding again Puddling spraying parcel 5 minutes, material will remain 20% in metering storage tank after mixing again Urea saturated solution is added in mixer on material, then will puddle after puddling 3 minutes uniform Material is put in storage bin hopper, then material conveyer is delivered to low temperature drier and carries out Low temperature drying, is transported to screening machine after drying and sieves, after screening finished product pack, check the weight, Package, put in storage.Residue on sieve waste material treatment in situ.

Embodiment 6

The organic master batch of described microorganism is prepared according to following steps

1. weigh 75 kilograms of yeast factory particle leftover bits and pieces, account for gross weight 75% and put metering storing into Struggle against stand-by.

2. weigh 1 kilogram of postindustrial sodium carbonate of pulverizing, account for gross weight 1% and put metering storing into Struggle against stand-by.

3. after weighing pulverizing, 5 kilograms of urea, MAP 2 kilograms, potassium sulfate 2 kilograms mix Compound, account for gross weight 9% put into metering storage bin hopper stand-by.

4. weigh 5 kilograms of magnesia, account for gross weight 2% put into metering storage bin hopper stand-by.

5. weigh bacillus amyloliquefaciens 0.1 kilogram, account for gross weight 0.1% and put metering storing into Struggle against stand-by.

6. weigh urea saturated solution 2.9 kilograms, account for gross weight 2.9% and put metering storage tank into Stand-by.

Add up to: 100 kilograms

First put leftover bits and pieces into mixer, in the case of being stirred continuously in mixer thing Material continuous uniform adds saturated aqueous solution of urea 2.4 kilograms (accounting for the 5/6 of total saturated solution), treats taste Essence leftover bits and pieces surface spraying is pressed upper in order continuously together with urea saturated solution after moistening uniformly State weight in mixer, add sodium carbonate, urea, MAP, potassium sulfate mixture, three Aoxidize two iron and bacillus amyloliquefaciens, puddle spraying parcel after adding again 5 minutes, thing Material is added to mixer by remaining 1/6 urea saturated solution in metering storage tank after mixing again In on material, more uniform material will be puddled after puddling 3 minutes and be put in storage bin hopper, Then material conveyer is delivered to low temperature drier and carry out low temperature drying, be transported to after drying Screening machine sieves, and after screening, finished product packs, checks the weight, package, puts in storage.Tail over Thing waste material treatment in situ.

Experimental example 1

1, demonstration place

Table 1.1, demonstration test ground distribution situation

2, exemplary materials

2.1, for examination fertilizer: organic master batch of preparation in embodiment 1.

2.2, various places are for studying thing and and kind

For studying thing: corn

Kind: Beilin District: prosperous No. 1；Mulan County: according to dragon 123；Shuangcheng City: good jade 99。

Three, exemplary design

Great Qu method of comparison is taked in demonstration test, does not set repetition.

Beilin District, if 2 process:

Process A: the conventional fertilizer application+2kg/ organic master batch of mu microorganism is puddled and uniformly made base fertilizer； Process B: conventional fertilizer application.(base fertilizer western Shandong corn fertilizer (22-13-10) 30kg, two Ammonium (18-46-0) 5kg, topdress grand celebration urea (46%) 15kg).

Processing A area is 10 mu；Processing B area is 5 mu.

Mulan County, if 3 process:

Process A: the conventional fertilizer application+2kg/ organic master batch of mu microorganism is puddled and uniformly made base fertilizer；Place Reason B: the conventional fertilizer application Liang Jianshi 4kg composite fertilizer+2kg/ organic master batch of mu microorganism；Process C: conventional fertilizer application.(Wo Fute Controlled Release Fertilizer 35kg, topdress grand celebration urea (46%) 15kg).

In place, A and each 20 mu of B of process, processes C area 5 mu.

Shuangcheng City, if 3 process:

Process A: the conventional fertilizer application+2kg/ organic master batch of mu microorganism is puddled and uniformly made base fertilizer；Place Reason B: conventional fertilizer application amount subtracts the 4kg/ mu+2kg/ organic master batch of mu microorganism and puddles and uniformly make base fertilizer, Urea 18kg is imposed before corn jointing.Process C: conventional fertilizer application.Use Miao Zhiyuan for every mu Board composite fertilizer 40kg, imposes urea 22kg before corn jointing.

The application process of fertilizer is machine sowing: be sown into corn seed at seeding time simultaneously, broadcasts Below seed at 7cm-10cm.

Four, field management

Each demonstration area cultivation condition (soil types, applying fertilizer, field management measure etc.) Keep consistent.Microorganism organic master batch usage: with composite fertilizer's (or conventional fertilizer application kind) is mixed Close uniformly, make base manure and use.Employ the special chemical herbicide of corn, just producing by locality Often field management.

Five, demonstration result and analysis

1, Beilin District

To corn growth stage investigation statistics such as table 1.2:

Table 1.2, corn growth stage application form

Process

Sowing

Emerge

Jointing

Take out hero

Weave silk

Ripe

Results

A

April 30

May 18

June 12

July 22

August 1

September 27

October 8

B(CK)

April 30

May 18

June 12

July 22

August 1

September 27

October 8

Investigating from breeding time, it is the poorest that process A does not sees with process B (comparison) project Not.

Autumn harvest is surveyed and is produced, and yield factors each to corn and economic benefit situation carry out investigation statistics knot Fruit is such as table 1.3 and table 1.4:

Table 1.3, corn yield result table

Illustrating: above field investigation data are triadic mean value, surveying and producing data is 5 mean values.

Table 1.4, yield result and performance analysis table

Note: the organic master batch of microorganism and corn, per kilogram respectively by 6.00 yuan, 1.60 yuan of calculating,

In terms of corn yield result, process A than processing B (comparison) at plant height, spike length, Grain number per spike is all increased slightly, increase respectively 18cm, 1cm, 20.Process the yield of A Adding 42.4 kilograms than processing B, increase production 6.3%, mu increases income 55.8 yuan.

2, Mulan County

To corn growth stage, yield and economic benefit investigation statistics such as table 1.5, table 1.6 and table 1.7:

Table 1.5, corn growth stage application form

Process

Sowing

Emerge

Jointing

Take out hero

Weave silk

Ripe

Results

A

April 26

May 14

June 8

July 20

July 28

September 26

October 5

B

April 26

May 14

June 8

July 21

July 29

September 28

October 5

C(CK)

April 26

May 14

June 8

July 21

July 29

September 28

October 5

Table 1.6, corn yield result table

Interpretation of result: process A from investigation breeding time and carry than processing C (comparison) jointing First 1 day；Taking out hero, weave silk 1 day in advance, the maturity period is advanced by 2 days, processes B and place Reason C is not changed in.

Survey in terms of product result from corn, process A than processing C (comparison) at plant height, spike length, Grain number per spike and 100-grain weight have increase to be 0.5cm, 2cm, 34,0.05g respectively；Make jade The bald point of rice has reduced, and decreases 1.5cm.Process B than processing C at plant height, spike length, 100-grain weight is not changed in.The yield processing A adds 41.62 kilograms than process C, processes B decreases 8.36 kilograms than processing C.

3, Shuangcheng City

Corn growth stage, biological character, yield and economic benefit to each demonstration test ground Carry out investigation statistics, concrete outcome and being analyzed as follows:

Breeding time is investigated: being found out by field investigation, the organic master batch of Yield-increasing Baallus In Maize microorganism can Promote maize growth process: compare on the maturity period, process A all ratios and process B and process C Early 2 days, refer to table 1.7.

Table 1.7, maize growth plan unit: the moon, day

Corn biological character investigation result: situation such as table 1.8:

Table 1.8, corn biological character investigation result list position: strain/m²、cm

Find out from table 1.8, plant height, spike length, bald point compare, process A and be better than place Reason B and process C: agriculture Fengzhen Bao Sheng peasant household Zhao Yu river, village exemplary treatments A difference on plant height Than processing B and C high 4cm, 5cm；Spike length compares the spike length of place A respectively than process Spike length long 0.4cm, 0.5cm of B and C；On bald point, comparison process A ratio respectively processes B 0.3cm, 0.4cm is reduced with C.Kui ground exemplary treatments A forever is closed in peasant household of Tong De village of township with one heart On plant height, ratio processes B and C high 3cm, 5cm respectively；Comparison process A on spike length Spike length respectively than process the long 0.3cm of spike length of B and C, 0.5；cm；Bald point compares place Reason A district ratio respectively processes B and C minimizing 0.6cm, 0.6cm.2 demonstration corns are biological Learn proterties the survey showed that, the organic master batch in embodiment 1 corn is had increase spike length, Reduce the effect of bald point.

Yield is investigated: surveying product method is to select representative field, selects 5 points, Every some area is 40 square metres, all gathers in the crops, and looks into spike number, whole threshing, calculates yield, Take the weighted average of 5, be finally converted into per mu yield.

Bao Sheng village test yield investigation result table 1.9:

Table 1.9, Bao Sheng village yield result comparison sheet unit: individual, g, kg, %

Compare in yield: process A per mu yield 931.1kg, process B per mu yield 877.2kg, process C per mu yield 874.2kg.Process A ratio and process B mu volume increase 53.9kg, amount of increase in production 6.1%, Process A ratio and process C mu volume increase 56.9kg, amount of increase in production 6.5%；Process B ratio and process C Mu volume increase 3.0kg, amount of increase in production 0.3%, being affixed by microorganism under the suitable decrement of conventional fertilizer application has The machine master batch not underproduction.

Tong De village test yield investigation result table 1.10:

Table 1.10, Tong De village yield result comparison sheet unit: individual, g, kg, %

Compare in yield: process A per mu yield 850.1kg, process B per mu yield 805.1kg, process C per mu yield 803.2kg；Process A ratio and process B mu volume increase 44.99kg, amount of increase in production 5.59%, Process A ratio and process C mu volume increase 46.89kg, amount of increase in production 5.84%；Process B ratio and process C Mu volume increase 1.93kg, amount of increase in production 0.24%, illustrate to be affixed by under the suitable decrement of conventional fertilizer application micro- Biological organic master batch is the not underproduction.

Economic and Efficiency Analysis: two demonstration tests are carried out Economic Benefit Accounting according to yield, Result such as table 1.11 (only adjusts fertilizers input, organic 6000.00 yuan/t of master batch, composite fertilizer 3200.00 yuan/t, 1900 yuan/t of urea, 1.80 yuan/kg of corn):

Table 1.11, Economic and Efficiency Analysis list position: kg, kg/ mu, unit

Found out by table 1.11, process A mu income the highest, be followed successively by process B and C；Protect Victory village exemplary treatments A increases income 76.62 yuan than process B mu, processes A and increases income than processing C mu 90.42 yuan, process B than processing C mu and increase income 13.80 yuan；Tong De village exemplary treatments A ratio Process B mu and increase income 60.60 yuan, process A and increase income 72.42 yuan than process C mu, process B 11.82 yuan are increased income than processing C mu.

Six, conclusion and suggestion

1, the organic master batch using the present invention can promote corn growth, to improving corn yield tool There is obvious effect.Four demonstration pilot project corns enrich microorganism on the basis of conventional fertilizer application Organic master batch has preferable effect of increasing production, and amount of increase in production is between 5.84%-6.5%.Meanwhile, Through demonstration checking, in the area that fertility is higher, can suitably reduce chemical fertilizer input and not Affect corn yield.

2, the organic master batch of microorganism using the present invention has certain economic effect to Maize Production Benefit, four demonstration pilot project data show, mu executes 2 kilograms of microbial inoculums, put into about 10 yuan, can be pure Increasing income 55.80 yuan-90.42 yuan, input and output are higher.

3, the organic master batch of microorganism using the present invention can improve soil, and the application of each demonstration pilot project should After product, loosing soil, soil texture is improved.

Experimental example 2

One, test objective

The checking microorganism organic master batch impact on Rice Production.

Two, test material and method

1. test site

Experiment and demonstration is arranged in county of three, Heilongjiang Province (district, city), specified place and soil base Plinth fertility state such as table 2.1:

Table 2.1, demonstration place and soil fertility situation

2. material to be tested

2.1 demonstration fertilizer: the organic master batch of microorganism prepared by method in embodiment 1.

2.2 demonstration rice varieties:

Table 2.2, various places demonstration rice varieties

Place	Kind
		Qin Jia town, Suihua Beilin District	Educate and select 8
Dong Jin town, Suihua Beilin District	2-117
		Liuhe town, Mulan County	The fragrant round-grained rice 1 of middle dragon
Shuangcheng City list cities and towns	The rice fragrance of a flower 2

3. exemplary treatments design

Use great Qu directly comparing method, do not set repetition.

3.1 Suihua Beilin Districts

Two demonstration pilot projects set 3 process the most respectively:

Process A: the conventional fertilizer application amount+2kg/ organic master batch of mu microorganism；Process B: conventional Dose subtracts executes the 4kg+2kg/ organic master batch of mu microorganism；Process C (comparison): conventional Fertilising.Wherein: Qin Jia town demonstration pilot project dose is 11.7 kgs/acre of urea, two ammoniums 20.0 Kg/acre, potassium sulfate 7.0 kgs/acre；Tianjin, east demonstration pilot project fertilizer amount is that urea 16.7 is public Pound/acre, two ammoniums 10.0 kgs/acre, potassium sulfate 10.0 kgs/acre.

Qin Jia town processes A area 1.8 mu, processes B area 2.4 mu, processes C face 15.8 Mu；Dong Jin town processes A area 2.6 mu, processes B area 4.6 mu, processes C area 17.8 Mu.

3.2 Mulan County

If 2 process:

Process A: the conventional fertilizer application amount+2kg/ organic master batch of mu microorganism；Process B (check plot): Conventional fertilizer application；Compound fertilizer dedicated to paddy (16-13-17) 25 kgs/acre makees base fertilizer, and tillering fertilizer is urinated Element 5 kgs/acre, 2.5 kgs/acre of fringe granulated fertilizer urea, 2.5 kgs/acre of potash fertilizer.

Process A area 14 mu, process B area 2 mu.

3.3 Shuangcheng City

If 2 process:

Process A: conventional fertilizer application+2kg every mu and use the organic master batch of microorganism；Process B (right According to district): conventional fertilizer application；Use holding up board paddy rice-dedicated fertilizer 35kg for every mu and make base fertilizer, at water Rice period of seedling establishment executes ammonium sulfate 5.5kg.

Process A area 15 mu, process B area 15 mu.Above four demonstration pilot project microbial inoculums make By method: the organic master batch of 2kg/ mu microorganism mixes with composite fertilizer's (or conventional fertilizer application kind) Uniformly, make base manure to use.

Three, field management

1. Suihua Beilin District: April 15 was sowed, and rice transplanting on May 15, fertilizer all makees base fertilizer Single use.Avoid after using organic master batch using other high toxicity bactericide as far as possible.Often 3 process other measures in addition to fertilization type, quantity difference of individual demonstration pilot project are the most identical, Use same kind, the identical amount of broadcasting, unified nursery, unified transplanting time and density, unified Irrigation period, unified weeding time and medicament, unification prevent and treat disease pest etc..

2. Mulan County: on April 18th, 2014 seeding and seedling raising (intelligent presoaking and germinating, booth Nursery)；Rice transplanting on May 23, the machine transplanting of rice, rice transplanting specification 9 × 4；May 27 spread fertilizer over the fields tiller Fertilizer, spread fertilizer over the fields fringe granulated fertilizer July 2, respectively processed in addition to fertilization type, quantity difference other Measure is the most identical.

3. Shuangcheng City: nursery on April 16, applied fertilizer to the subsoil May 22, the application process of fertilizer For: fertilizer is disposably spread fertilizer over the fields in rice terrace, then ploughs, May 23 soaked field, Rice transplanting on May 26, gathered in the crops September 30.Respectively process in addition to fertilization type, quantity difference, Other measures are the most identical.

Four, pilot survey and analysis

1, investigation breeding time

Table 2.3, Rice Growing application form in period

By each process has been carried out the field investigation of growthdevelopmental stage, paddy rice microbe Its growing process is not had much affect by organic master batch, and each processes growth period duration of rice basic one Causing, Shuangcheng City paddy rice processes A the most ripe about one day.

2. biological character investigation

2.1 Suihua Beilin Districts:

Table 2.4, Suihua Beilin District Qin Jia town field investigation result

Project	Process A	Process B	Process C
				Plant height cm	100	96	86
Individual plant secondary root	21	19	17
				Individual plant tiller	4.1	4.1	3.98
The average thick mm of stem	6.0	5.5	5.1
				Mean blade width mm	100	96	86
The long cm of main root	21.0	21.0	18.0
				The thick mm of average root	0.90	0.80	0.60
Diseased plant rate %	0	0.05	1.3
				Leaf look	Dark green	Light green	Yellowish

Table 2.5, Suihua Beilin District Dong Jin town field investigation result

July 22 carried out field investigation to two demonstration pilot projects, situation such as table 2.4 and table 2.5. In terms of model field range estimation and field paddy rice growing way condition survey result, the organic master batch of microorganism exists Having used certain growth-promoting effect on paddy rice, shown as plant height and increase, individual plant secondary root increases, Tiller is increased slightly, and average stem is thick, Ye Kuan, root are thick etc. also increased, and leaf look has necessarily Difference, shows dark green.

2.2 Mulan County:

Mulan County field investigation situation such as table 2.6, knowable to investigation result, using microorganism has The process A of machine master batch is superior to process B, explanation in the proterties such as plant height, thick, the root length of stem Use the organic master batch of microorganism and have obvious growth-promoting effect.

Table 2.6, Mulan County field investigation result

2.3 Shuangcheng City

Shuangcheng City field investigation situation such as table 2.7, knowable to investigation result, using microorganism has Than processing, the high 6cm of B, stem be thick, the long 2cm of main root length on plant height for the process A of machine master batch, Illustrate that using microbial inoculum has obvious growth-promoting effect.

Table 2.7, Shuangcheng City field investigation result

3, yield investigation

3.1 Suihua Beilin Districts:

2 demonstration pilot projects of Qin Jia and Dong Jin are carried out by September 27th, 2014 and 29 days respectively Survey and produce sampling, and in having carried out indoor species test 14-17 day in October, survey product result and be summarized as follows Table.

Table 2.8, Suihua Beilin District yield are investigated

Note: data above is triadic mean value.

In terms of yield component, Qin Jia town demonstration pilot project shows as processing A, processing B with right Comparing according to processing C, every square metre of paddy rice spike number is many, blighted grain number is few, but fringe granulating number, The factors such as mass of 1000 kernel do not change significantly.Dong Jin town demonstration pilot project processes A and outperforms process B and control treatment C, and process B and show every yield forming factor than control treatment C Index slightly below processes C.In terms of yield result, Qin Jia town demonstration pilot project rice application microorganism Organic master batch effect of increasing production is obvious, is affixed by this microbial inoculum on the basis of conventional fertilizer application, than comparison mu Increasing production 54.7 kilograms, amplification reaches 10.6%, and conventional fertilizer application every mu decrement 4 kilograms is affixed by micro- Biological organic master batch increases production 23.5 kilograms than comparison mu, amplification 4.6%.Dong Jin town demonstration pilot project Conventional fertilizer application is affixed by the organic master batch of microorganism, increases production 34.9 kilograms than comparison mu, and amplification reaches 7.0%, and conventional fertilizer application every mu subtracts and executes 4 kilograms, is affixed by the process ratio of the organic master batch of microorganism The comparison underproduction, reason is that this demonstration ground soil fertility is relatively low, and conventional fertilizer application amount is the highest, then Every mu subtracts and executes 4 kilograms, causes fertilizer supply amount to reduce the too much underproduction.

3.2 Mulan County:

Field was surveyed and was produced September 25, often processes and takes 3 points, and every some 1m2 investigates plant height, fringe Length, number of grain per ear, real grain number, sterile grain rate, mass of 1000 kernel etc., situation refers to table.

Table 2.9, Mulan County yield are investigated

From investigation result, process A plant height and spike length higher than processing B, square metre spike number, Number of grain per ear and setting percentage process A and are superior to process B.From yield result, process A relatively processes B mu volume increase 40.6kg, and rate of growth reaches 9.13%.

3.3 Shuangcheng City

Carrying out harvest time surveying and produce, surveying product method is: selects representative field, selects 5 points, every some area is 20 square metres, all gathers in the crops, and whole threshings, calculating are produced Amount, takes the weighted average of 5, is finally converted into per mu yield.

Table 2.10, Shuangcheng City yield are investigated

From table 2.10 it can be seen that process A (demonstration area) average yield per mu 743.7kg, place Reason B (check plot) average yield per mu 698.3kg.Process A ratio and process the average every mu of volume increase of B 45.4kg, amount of increase in production 6.50%.

4. Economic and Efficiency Analysis

4.1 Suihua Beilin Districts

Table 2.11, economic benefit statistics list position, demonstration ground, Suihua Beilin District: unit

Note: urea, two ammoniums, potassium sulfate, microorganism master batch microbial inoculum, paddy rice per kilogram respectively by 1.80 yuan, 3.50 yuan, 4.00 yuan, 6.00 yuan, 3.00 yuan of calculating, data above is triadic mean value.

From table 2.11, paddy rice microbe organic master batch input-output ratio does not has substantially Changing, high fertility district relatively compares slightly higher, and wherein processing A is 1:13.05, processes C and is 1:12.99；Zhong Di fertility district relatively compares lower slightly, and wherein processing A is 1:13.72, processes C is 1:14.29.Process A from increasing income amplitude Kan Qinjia town demonstration pilot project, process B than comparison Process C shows and increases income；Dong Jin town demonstration pilot project processes A and increases income than control treatment C performance, Process B and show the reduction of income than control treatment C.

4.2 Mulan County

Table 2.12, Economic and Efficiency Analysis list position, Mulan County: kg, kg/ mu, unit

Note: only adjust fertilizers input, microorganism 6 yuan/kg of organic master batch, 4 yuan/kg of rice

Processing A and increase income 150.4 yuan than process B mu, the effect of increasing income is obvious.

4.3 Shuangcheng City

Table 2.13, Economic and Efficiency Analysis list position: kg, kg/ mu, unit

Note: only adjust fertilizers input, microorganism 6000.00 yuan/t of organic master batch, holding up board is multiple 3200.00 yuan/the t in Hefei, 1400 yuan/t of ammonium sulfate, 4.8 yuan/kg of paddy rice.

By table 2.13 it can be seen that process A to increase income 206 yuan than process B every mu, increase Fruit of producing effects is notable.

Five, conclusions and recommendations

1, use the organic master batch of microorganism and can promote paddy growth, have improving rice yield Obvious effect.4 demonstration pilot project paddy rice enrich microorganism on the basis of conventional fertilizer application to be had Machine master batch all has good effect of increasing production under which kind of soil fertility condition.Prove through demonstration, In the area that fertility is higher, can suitably reduce the input of chemical fertilizer and not affect rice yield, And in the most barren area of soil, chemical fertilizer subtracts to execute wants prudent.

2, use microorganism organic master batch and Rice Production is had certain economic benefit, 4 Demonstration pilot project data show, mu executes 2 kilograms of microbial inoculums, put into about 10 yuan, pure can increase income 92 -206 yuan of unit, input and output are higher.

3, use the organic master batch of microorganism and can improve soil, after each demonstration pilot project applies this product, Loosing soil, soil texture obtains a certain degree of improvement.

Although illustrate and describing the present invention with specific embodiment, but it will be appreciated that May be made that without departing from the spirit and scope of the present invention many other change and Amendment.It is, therefore, intended that include in the following claims belonging in the scope of the invention All such changes and modifications.

Claims (10)

1. the organic master batch of microorganism, it is characterised in that described microorganism organic master batch master To be made up of following components by weight percentage:

Trade waste: 65-85%；

Industrial alkali raw material: 0-10%；

Agricultural fertilizer: 0-20%；

Activating agent: 1-10%；

Microbial bacterial agent: 0.1-10%；

Liquid adjuvants 2-5%.

The organic master batch of microorganism the most according to claim 1, it is characterised in that described The organic master batch of microorganism is mainly made up of following components by weight percentage:

Trade waste: 70-75%；

Industrial alkali raw material: 6-9%；

Agricultural fertilizer: 10-20%；

Activating agent: 1-5%；

Microbial bacterial agent: 0.1-2%；

Liquid adjuvants 3-4.5%.

The organic master batch of microorganism the most according to claim 1, it is characterised in that described Trade waste is under Gourmet Powder Factory's particle leftover bits and pieces, yeast factory particle leftover bits and pieces and sugar refinery particle One or more in heel, it is preferable that described trade waste is industry Gourmet Powder Factory particle Leftover bits and pieces.

The organic master batch of microorganism the most according to claim 1, it is characterised in that described Industrial alkali raw material is industrial monohydrate, sodium acid carbonate, potassium carbonate, wood shavings alkali, hydrogen-oxygen Change one or more in potassium and NaOH, it is preferable that described industrial alkali raw material is carbon Acid sodium or potassium carbonate.

The organic master batch of microorganism the most according to claim 1, it is characterised in that described Agricultural fertilizer is urea, ammonium sulfate, ammonium hydrogen carbonate, MAP, Diammonium phosphate (DAP), phosphoric acid One or more in potassium dihydrogen, potassium sulfate and potassium chloride, it is preferable that described agricultural fertilizer For one or more in urea, MAP, potassium sulfate.

The organic master batch of microorganism the most according to claim 1, it is characterised in that described Activating agent is in ferroso-ferric oxide, di-iron trioxide, carbon black, magnesia and copper sulphate Kind, it is preferable that described activating agent is ferroso-ferric oxide or di-iron trioxide.

The organic master batch of microorganism the most according to claim 1, it is characterised in that described Microbial bacterial agent be bacillus subtilis, bacillus amyloliquefaciens, bacillus megaterium, One or more in clothing bacillus and colloid bacillus cereus, it is preferable that described microorganism Microbial inoculum is bacillus subtilis, bacillus amyloliquefaciens, colloid bacillus cereus or withered grass gemma Bacillus, bacillus amyloliquefaciens and the bacterium mixture of colloid gemma bar.

The organic master batch of microorganism the most according to claim 1, it is characterised in that described Liquid adjuvants is urea saturated aqueous solution, fusing urea, molten sulfur sodium thiosulfate, melts five One or many in water sodium metasilicate, melted non-hydrate sodium metasilicate and polyacrylamide solution Kind, it is preferable that described liquid adjuvants is urea saturated aqueous solution.

9. the preparation side of the organic master batch of microorganism described in any one in claim 1-8 Method, it is characterised in that said method comprising the steps of:

1) trade waste is added in container, add partially liq auxiliary agent under agitation；

2) industrial alkali raw material, agricultural fertilizer, activating agent, microbial bacteria it are continuously added to successively Agent, stirs 3-5 minute after adding；

3) add remaining liq auxiliary agent, continue stirring 2-3 minute；

4) it is dried and sieves；

Wherein, described step 1) in the liquid adjuvants and the described step 3 that add) in add Liquid adjuvants, volume ratio is 3-5:1.

Method the most according to claim 9, it is characterised in that described step 4) In dry be under normal temperature and pressure air-dry；It is little that described master batch is sized to particle diameter by described screening In 4mm.