CN105747047A - Anti-haze drink capable of reducing haze-induced lung injuries and preparation method of anti-haze drink - Google Patents

Anti-haze drink capable of reducing haze-induced lung injuries and preparation method of anti-haze drink Download PDF

Info

Publication number
CN105747047A
CN105747047A CN201610164630.1A CN201610164630A CN105747047A CN 105747047 A CN105747047 A CN 105747047A CN 201610164630 A CN201610164630 A CN 201610164630A CN 105747047 A CN105747047 A CN 105747047A
Authority
CN
China
Prior art keywords
parts
haze
raw material
lung
semen
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN201610164630.1A
Other languages
Chinese (zh)
Inventor
陈波
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Priority to CN201610164630.1A priority Critical patent/CN105747047A/en
Publication of CN105747047A publication Critical patent/CN105747047A/en
Withdrawn legal-status Critical Current

Links

Classifications

    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/02—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation containing fruit or vegetable juices
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Their preparation
    • A23L2/52—Adding ingredients
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
    • A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs

Abstract

The invention discloses an anti-haze drink capable of reducing haze-induced lung injuries and a preparation method of the anti-haze drink. The anti-haze drink is prepared from effective raw materials and food additives, wherein the effective raw materials comprise 5-15 parts of honeysuckle flowers, 10-20 parts of platycodon roots, 15-25 parts of grosvenor momordica fruits, 4-8 parts of mint, 2-4 parts of bitter gourd vines, 10-20 parts of Chinese dates, 10-20 parts of Chinese wolfberries, 6-10 parts of hairy asiabell roots and 2-4 parts of radish seeds and Chinese velvet beans in total, and the weight ratio of the radish seeds to the Chinese velvet beans is (1-3):1. Experiments indicate that PM2.5-induced lung injury animal model mice have limited respiratory functions, particularly dysfunction of small airways, airway resistance is increased obviously, and the probability of airway obstruction exists. After treatment with the anti-haze drink, lung functions of the mice are improved, particularly reduction of the airway resistance and increase of the airway compliance, therefore, the anti-haze drink can reduce PM2.5-induced lung function injuries, reduce the airway resistance and improve the lung compliance.

Description

A kind of reduce haze anti-haze beverage causing injury of lung and preparation method thereof
Technical field
The invention belongs to field of food, relate to anti-haze beverage, be specifically related to a kind of reduce anti-haze beverage causing injury of lung of PM2.5 in haze and preparation method thereof.
Background technology
In recent years along with the quickening of economic fast development and urbanization process, China's most area occurs in that serious haze pollutes, and the generation of haze weather also exists close associating with fine particle PM2.5 concentration in air.The burying that aerodynamic size is little and specific surface area the is big specialization of PM2.5 is more special; make the poisonous and harmful substances such as its more portable motor-vehicle tail-gas, big capacity industrial waste gas, volatile chemical; the filtration part of vibrissa loses protective effect; and make a large amount of poisonous and harmful substance enter human lung tissue depths and blood circulation through respiratory tract, health is produced serious harm.So world health knit recommend PM2.5 concentration be used as detection air quality index.1997, EPA redefined the index of PM2.5 upper limit of concentration in " NAAQS ", and atmospheric environment upper limit of concentration Sino-Japan, average annual respectively changes 65 μ g/m3、15μg/m3, and in 2006 by average daily upper limit of concentration by 65 μ g/m3It is adjusted downward to 35 μ g/m3.Due to PM2.5 concentration over-standard, cause people to healthy worry.
Current study show that, PM2.5 environmental pollution appreciable impact respiratory disorder sickness rate, be in hospital Lee, mortality rate, owing to it is deposited in alveolar, it is prone to stimulate and corrosion alveolar wall generation inflammatory reaction, cause lung tissue structure's damage and dysfunction, destroy defensive barrier and self anti-weighing apparatus function of pulmonary macrophage, reduce pulmonary function, cause dramatically increasing of respiratory system disease, such as pneumonia, chronic bronchitis, chronic obstructive pulmonary disease, bronchial asthma, coughing occurs in patient, disease, the Respiratory symptoms such as breathe heavily.
For current present situation, if it is possible to the anti-haze beverage that develop a excellent effect, PM2.5 can be reduced respiratory system is damaged, the favor in market will be subject to.
Summary of the invention
The first object of the present invention is in that a kind of PM2.5 of reduction causes the anti-haze beverage of injury of lung, for reducing the PM2.5 damage to body pulmonary, improves the ability of body fight PM2.5；
The second object of the present invention is in that the preparation method providing above-mentioned anti-haze beverage.
The above-mentioned purpose of the present invention is achieved by the techniques below scheme:
A kind of PM2.5 of reduction causes the anti-haze beverage of injury of lung, and including Effect raw material and food additive, described Effect raw material includes: Flos Lonicerae, 5～15 parts；Radix Platycodonis, 10～20 parts；Fructus Momordicae, 15～25 parts；Herba Menthae, 4～8 parts；Caulis Momordicae Charantiae, 2～4 parts；Fructus Jujubae, 10～20 parts；Fructus Lycii, 10～20 parts；Radix Codonopsis, 6～10 parts；Semen Raphani and Semen stizolobii capitati totally 2～4 parts；Wherein, the weight ratio of described Semen Raphani and Semen stizolobii capitati is 1～3:1.
Further, the described PM2.5 that reduces causes the anti-haze beverage of injury of lung, and including Effect raw material and food additive, described Effect raw material includes: Flos Lonicerae, 10 parts；Radix Platycodonis, 15 parts；Fructus Momordicae, 20 parts；Herba Menthae, 6 parts；Caulis Momordicae Charantiae, 3 parts；Fructus Jujubae, 15 parts；Fructus Lycii, 15 parts；Radix Codonopsis, 8 parts；Semen Raphani and Semen stizolobii capitati totally 3 parts；Wherein, the weight ratio of described Semen Raphani and Semen stizolobii capitati is 2:1.
Further, the described PM2.5 that reduces causes the anti-haze beverage of injury of lung, and including Effect raw material and food additive, described Effect raw material includes: Flos Lonicerae, 5 parts；Radix Platycodonis, 10 parts；Fructus Momordicae, 15 parts；Herba Menthae, 4 parts；Caulis Momordicae Charantiae, 2 parts；Fructus Jujubae, 10 parts；Fructus Lycii, 10 parts；Radix Codonopsis, 6 parts；Semen Raphani and Semen stizolobii capitati totally 2 parts；Wherein, the weight ratio of described Semen Raphani and Semen stizolobii capitati is 1:1.
Further, the described PM2.5 that reduces causes the anti-haze beverage of injury of lung, and including Effect raw material and food additive, described Effect raw material includes: Flos Lonicerae, 15 parts；Radix Platycodonis, 20 parts；Fructus Momordicae, 25 parts；Herba Menthae, 8 parts；Caulis Momordicae Charantiae, 4 parts；Fructus Jujubae, 20 parts；Fructus Lycii, 20 parts；Radix Codonopsis, 10 parts；Semen Raphani and Semen stizolobii capitati totally 4 parts；Wherein, the weight ratio of described Semen Raphani and Semen stizolobii capitati is 3:1.
Further, described food additive includes sweeting agent and other acceptable food additive.
Further, described sweeting agent is saccharide and/or non-sugar sweetener.
Further, described sugar sweetener is selected from one or more in white sugar, brown sugar, brown sugar, nigecose, high fructose syrup, sucrose, fructose, glucose, maltose, Mel.
Further, described non-sugar sweetener is selected from one or more in cyclamate, acesulfame potassium, sorbitol, xylitol, maltose alcohol, glycyrrhizin, stevioside, aspartame, sucralose.
The preparation method of above-mentioned anti-haze beverage, comprises the steps:
Step S1, cleans above-mentioned Effect raw material, dries, add 15～25 times amount pure water, decoct 90～120 minutes, filter, obtain filtrate；Filtering residue adds 15～25 times amount pure water heating extraction 60～90 minutes again, filters, obtains filtrate；Twice filtrate merging is concentrated into every 25ml extracting solution containing Effect raw material 1g；
Step S2, dilutes extracting solution pure water, adds food additive, dissolve, boil, sterilizing, filter, subpackage.
Advantages of the present invention:
Experiments show that, PM2.5 causes injury of lung animal model mice, and to there is ventilatory function limited, the especially dysfunction of small airway, and air flue resistance, for substantially increasing, exists the probability of airway obstruction.After haze beverage provided by the invention is treated, the pulmonary function of mice improves, main manifestations is the increase of the reduction of airway resistance, air flue compliance, illustrates that haze beverage provided by the invention can improve the PM2.5 pulmonary function injury caused, reduces the resistance of air flue, improves lung compliance.
Detailed description of the invention
Further illustrate the essentiality content of the present invention below in conjunction with embodiment, but do not limit scope with this.Although the present invention being explained in detail with reference to preferred embodiment, it will be understood by those within the art that, it is possible to technical scheme is modified or equivalent replacement, without deviating from the spirit and scope of technical solution of the present invention.
Flos Lonicerae is the Alabastrum or just opened flower of caprifoliaceae plant Flos Lonicerae.Fructus Momordicae is the fruit of cucurbitaceous plant Fructus Momordicae.Herba Menthae is herb or the leaf of labiate Herba Menthae.Caulis Momordicae Charantiae is the stem of cucurbitaceous plant Fructus Momordicae charantiae.Radix Codonopsis is the root of Campanulaceae Radix Codonopsis.Semen Raphani is the dry mature seed of crucifer Radix Raphani.Tap plant during fruit maturation in summer, dry, rub seed, remove impurity, then dry.Semen stizolobii capitati is the seed of leguminous plant headdress flower Semen stizolobii capitati.Gather during fruit maturation after autumn.Above-mentioned raw materials dries, and water content is less than 5%.
Embodiment 1: the preparation of haze beverage
Effect raw material weight ratio:
Flos Lonicerae, 10 parts；Radix Platycodonis, 15 parts；Fructus Momordicae, 20 parts；Herba Menthae, 6 parts；Caulis Momordicae Charantiae, 3 parts；Fructus Jujubae, 15 parts；Fructus Lycii, 15 parts；Radix Codonopsis, 8 parts；Semen Raphani and Semen stizolobii capitati totally 3 parts；Wherein, the weight ratio of Semen Raphani and Semen stizolobii capitati is 2:1.
The preparation method of haze beverage:
Step S1, cleans above-mentioned Effect raw material, dries, add 20 times amount pure water, decoct 120 minutes, filter, obtain filtrate；Filtering residue adds 20 times amount pure water heating extraction 90 minutes again, filters, obtains filtrate；Twice filtrate merging is concentrated into every 25ml extracting solution containing Effect raw material 1g；
Step S2, dilutes 10 times by extracting solution pure water, adds 1.5% white sugar (w/v), dissolve, boil, 120 DEG C of autoclavings, filter, subpackage.
Embodiment 2: the preparation of haze beverage
Effect raw material weight ratio:
Flos Lonicerae, 5 parts；Radix Platycodonis, 10 parts；Fructus Momordicae, 15 parts；Herba Menthae, 4 parts；Caulis Momordicae Charantiae, 2 parts；Fructus Jujubae, 10 parts；Fructus Lycii, 10 parts；Radix Codonopsis, 6 parts；Semen Raphani and Semen stizolobii capitati totally 2 parts；The weight ratio of described Semen Raphani and Semen stizolobii capitati is 1:1.
The preparation method of haze beverage:
Step S1, cleans above-mentioned Effect raw material, dries, add 20 times amount pure water, decoct 120 minutes, filter, obtain filtrate；Filtering residue adds 20 times amount pure water heating extraction 90 minutes again, filters, obtains filtrate；Twice filtrate merging is concentrated into every 25ml extracting solution containing Effect raw material 1g；
Step S2, dilutes 10 times by extracting solution pure water, adds 1.5% white sugar (w/v), dissolve, boil, 120 DEG C of autoclavings, filter, subpackage.
Embodiment 3: the preparation of haze beverage
Effect raw material weight ratio:
Flos Lonicerae, 15 parts；Radix Platycodonis, 20 parts；Fructus Momordicae, 25 parts；Herba Menthae, 8 parts；Caulis Momordicae Charantiae, 4 parts；Fructus Jujubae, 20 parts；Fructus Lycii, 20 parts；Radix Codonopsis, 10 parts；Semen Raphani and Semen stizolobii capitati totally 4 parts；The weight ratio of described Semen Raphani and Semen stizolobii capitati is 3:1.
The preparation method of haze beverage:
Step S1, cleans above-mentioned Effect raw material, dries, add 20 times amount pure water, decoct 120 minutes, filter, obtain filtrate；Filtering residue adds 20 times amount pure water heating extraction 90 minutes again, filters, obtains filtrate；Twice filtrate merging is concentrated into every 25ml extracting solution containing Effect raw material 1g；
Step S2, dilutes 10 times by extracting solution pure water, adds 1.5% white sugar (w/v), dissolve, boil, 120 DEG C of autoclavings, filter, subpackage.
Embodiment 4: the preparation of haze beverage
Effect raw material weight ratio:
Flos Lonicerae, 10 parts；Radix Platycodonis, 15 parts；Fructus Momordicae, 20 parts；Herba Menthae, 6 parts；Caulis Momordicae Charantiae, 3 parts；Fructus Jujubae, 15 parts；Fructus Lycii, 15 parts；Radix Codonopsis, 8 parts；Semen Raphani and Semen stizolobii capitati totally 3 parts；Wherein, the weight ratio of Semen Raphani and Semen stizolobii capitati is 1:1.
The preparation method of haze beverage:
Step S1, cleans above-mentioned Effect raw material, dries, add 20 times amount pure water, decoct 120 minutes, filter, obtain filtrate；Filtering residue adds 20 times amount pure water heating extraction 90 minutes again, filters, obtains filtrate；Twice filtrate merging is concentrated into every 25ml extracting solution containing Effect raw material 1g；
Step S2, dilutes 10 times by extracting solution pure water, adds 1.5% white sugar (w/v), dissolve, boil, 120 DEG C of autoclavings, filter, subpackage.
Embodiment 5: the preparation of haze beverage
Effect raw material weight ratio:
Flos Lonicerae, 10 parts；Radix Platycodonis, 15 parts；Fructus Momordicae, 20 parts；Herba Menthae, 6 parts；Caulis Momordicae Charantiae, 3 parts；Fructus Jujubae, 15 parts；Fructus Lycii, 15 parts；Radix Codonopsis, 8 parts；Semen Raphani and Semen stizolobii capitati totally 3 parts；Wherein, the weight ratio of Semen Raphani and Semen stizolobii capitati is 3:1.
The preparation method of haze beverage:
Step S1, cleans above-mentioned Effect raw material, dries, add 20 times amount pure water, decoct 120 minutes, filter, obtain filtrate；Filtering residue adds 20 times amount pure water heating extraction 90 minutes again, filters, obtains filtrate；Twice filtrate merging is concentrated into every 25ml extracting solution containing Effect raw material 1g；
Step S2, dilutes 10 times by extracting solution pure water, adds 1.5% white sugar (w/v), dissolve, boil, 120 DEG C of autoclavings, filter, subpackage.
Embodiment 6: the weight ratio of the contrast of embodiment 1, Semen Raphani and Semen stizolobii capitati is 0.5:1
Effect raw material weight ratio:
Flos Lonicerae, 10 parts；Radix Platycodonis, 15 parts；Fructus Momordicae, 20 parts；Herba Menthae, 6 parts；Caulis Momordicae Charantiae, 3 parts；Fructus Jujubae, 15 parts；Fructus Lycii, 15 parts；Radix Codonopsis, 8 parts；Semen Raphani and Semen stizolobii capitati totally 3 parts；Wherein, the weight ratio of Semen Raphani and Semen stizolobii capitati is 0.5:1.
The preparation method of haze beverage:
Step S1, cleans above-mentioned Effect raw material, dries, add 20 times amount pure water, decoct 120 minutes, filter, obtain filtrate；Filtering residue adds 20 times amount pure water heating extraction 90 minutes again, filters, obtains filtrate；Twice filtrate merging is concentrated into every 25ml extracting solution containing Effect raw material 1g；
Step S2, dilutes 10 times by extracting solution pure water, adds 1.5% white sugar (w/v), dissolve, boil, 120 DEG C of autoclavings, filter, subpackage.
Embodiment 7: the weight ratio of the contrast of embodiment 1, Semen Raphani and Semen stizolobii capitati is 3.5:1
Effect raw material weight ratio:
Flos Lonicerae, 10 parts；Radix Platycodonis, 15 parts；Fructus Momordicae, 20 parts；Herba Menthae, 6 parts；Caulis Momordicae Charantiae, 3 parts；Fructus Jujubae, 15 parts；Fructus Lycii, 15 parts；Radix Codonopsis, 8 parts；Semen Raphani and Semen stizolobii capitati totally 3 parts；Wherein, the weight ratio of Semen Raphani and Semen stizolobii capitati is 3.5:1.
The preparation method of haze beverage:
Step S1, cleans above-mentioned Effect raw material, dries, add 20 times amount pure water, decoct 120 minutes, filter, obtain filtrate；Filtering residue adds 20 times amount pure water heating extraction 90 minutes again, filters, obtains filtrate；Twice filtrate merging is concentrated into every 25ml extracting solution containing Effect raw material 1g；
Step S2, dilutes 10 times by extracting solution pure water, adds 1.5% white sugar (w/v), dissolve, boil, 120 DEG C of autoclavings, filter, subpackage.
Embodiment 8: effect example, causes the improvement result of injury of lung to PM2.5
One, material and method
1, material
1.1 experimental animals
Healthy ICR female mice 60, body weight 25～27g (limited department provides by Beijing China Fukang biotechnology share).Temperature 22～28 DEG C, the cleaning ambient of relative humidity 50～60% is raised, 5 cages, adopt 12/12 illumination.
1.2 samples
In embodiment 1～7 preparation method step S1 obtain extracting solution (every 25ml is containing Effect raw material 1g), be labeled as successively respectively sample 1.～7., gastric infusion.
1.3 instrument and equipments
(1) Anderson type particle sampler HBL-GUV:Thermo company
(2) particulate matter real-time detector TEOM1400a: Rupprecht&Patashnik company of the U.S.；
(3) particle sampler ACCU: Rupprecht&Patashnik company of the U.S.；
(4) DustTrak particulate matter visualizer: Technical Sourcing Internation of the U.S.；
(5) quartz fibre filter membrane: Beijing Sai Fulaibo Science and Technology Ltd.；
(6) SK3300LH-ultrasonator (operating frequency 40/59K hertz)；
(7) low-temperature freeze-drying machine (Beijing)；
(8) AniRes2005 animal lung function analyzes system: Beijing Bei Lanbo Science and Technology Ltd.；
(9) microscope (Olympus)；
(10) CR313 refrigerated centrifuger: France Jouan；
(11)-20 DEG C of refrigerator-freezers: China's Haier；
(12) 4 DEG C of refrigerators: China's Haier；
(13) micropipettor: Germany Eppendorf；
(14) electronic balance: Minqiao Precision Scientific Instruments Co., Ltd., Shanghai.
2, method
The collection of 2.1PM2.5 and preparation
2.1.1 sampling position and the time
According to road and the traffic related information situation of changing partial loop and periphery highway in the district of capital, and each ring motor vehicles there won't be any problem with restricted driving measure, choose the Fourth Ring road collection point as this experiment PM2.5.Because Fourth Ring road traffic flow is big in normal conditions, the various feature that can represent urban road of motor vehicles type, the change of its transport structure can be good at reflecting the road traffic condition of Beijing, can as the Typical Representative of Beijing city road.This research selects between stage casing, North 4th Ring Road Bao Fu temple bridge and institute's bridge as sampling section, particulate matter observed samples point requires to be arranged at the south of road or the north of a road according to wind direction and city management, the distance that the sampled point in the south of road and the north of a road runs from road has notable difference, north of a road multiple spurs road outer rim is about 5m, and south of road multiple spurs road outer rim is about 2m.Sampling time is in December, 2014～2015 year 01 month, mainly winter.
2.1.2 the method for sampling and record
This research adopt the real-time basket of Anderson type particle sampler HBL-GUV, particulate matter survey instrument TEOM1400a, ACCU and PM2.5 sickle with the use of, sampling membrane is clean stone cottonrose hibiscus fibrous filter membrane, carries out, with ready filter membrane, sample (having been cooperated by Environmental Science and Engineering institute of Tsing-Hua University).Having recorded the change of meteorologic parameter near sampled point ingeniously, mainly had temperature, relative humidity, wind direction, wind speed and precipitation etc., 24h changes a filter membrane, take off filter membrane and carry out skilful (dirt face in), put into the sealing bag of the number of finishing, rearmounted 4 DEG C of refrigerators preserve, standby.
2.1.3PM2.5 the preparation of suspension
(1) being stopped up offer by Tsing-Hua University's environmental experiment and collect in December, 2014～2015 year, Beijing Atmospheric particulates of 01 month, main use quartz fibre film is collected, and every film is containing about PM2.5150mg, and a film takes therein 1/2, shares 10 films.(75mg × 10=750mg)；
(2) respectively 10 films are divided into 3 parts with tissue shear；
(3) a film is cut into the module of 1.0cm × 0.5cm size, is attached separately in 3 500ml brown, wide-mouth bottles；
(4) 250ml deionized water poured into by each brown, wide-mouth bottle, by unrestrained for quartz film leaching；
(5) will be equipped with the wide mouthed bottle of quartz film and be placed in ultrasonic vibration machine, 40min × 3 time, fully concussion, eluting particles thing；
(6) concussion liquid is through eight layers of filtered through gauze, and the abundant press filtration of deionized water being with by quartz film, takes filtrate；
(7) 3 bottles of filtrates are dried through vacuum drier, evaporate, dried PM2.5 normal saline is made into desired concn, 6mg/ml.
(8) PM2.5 suspension is through autoclaving 15min, deposits in 4 DEG C of environment standby, with front sonic oscillation 5min so that particulate matter mixes
2.2PM2.5 causes mouse lung Establishing an injured model and packet
2.2.1 it is grouped
Healthy ICR female mice 180, is randomly divided into 9 groups by body weight: Normal group, model comparison are prevented, sample 1. group～sample 7. group, often group 20.Except preventing except normal control, all the other each PM2.5 that are all fabricated to cause model of lung injury.
2.2.2 Animal Model
The all adaptive raising of each group one week.
Blank group: setting up the 8th, 14,20 and 26 day via intranasal application respectively at model and instill 0.9% normal saline (20 μ l/ are only), all the other times normally raise.
Model control group: adopt nasal cavity instillation PM2.5 suspension method, set up the 8th, 14 respectively at model, the PM2.5 suspension that via intranasal application instillation in 20 and 26 days prepares, 40mg/kg bw, calculate by 30g body weight, about 1.2mg/20 μ l/ only, put to death in latter 48 hours by last contamination.
Sample is group～sample 7. group 1.: the same model control group of method for establishing model.
2.3 route of administration and method
Blank group, model control group: from modeling, administered by oral gavage 0.9% normal saline 0.2ml//d, continuous 3 weeks.
Sample is group～sample 7. group 1.: from modeling, respectively administered by oral gavage sample 1.～7. 0.9% normal saline 0.2ml//d, continuous 3 weeks.
2.4 lung function
(1) 1% pentobarbital intraperitoneal injection of anesthesia mice (0.15ml/30g) so that it is deep anesthesia as far as possible, in order to avoid autonomous respiration is resisted mutually with respirator, affects result；
(2) taking dorsal position due to operating board, cut off skin of neck with tissue shear, tissue clamps chorista exposes trachea blunt separation trachea；
(3) a kerf is cut in the nearly head place of trachea, about 1.0mm, inserts trachea by the gas-tpe fitting place intubated, fixes with cotton thread.
(4) transfer mice to body retouches instrument platform；
(5) assisted respiartion machine and gas-tpe fitting are connected；
(6) change of the index such as pulmonary function RLMax, ReMax, RLArea, ReArea, CdynMax, CdynArea, PEF of mice is recorded.(air-breathing/breath pressure of laboratory animal all has respirator to provide, and makes maintenance steady state).
2.5 statistical analysiss
All data all adopt SPSS20.0 statistical analysis software to carry out statistical procedures.
3, experimental result
The long-term PM2.5 that sucks can stimulate the recurrent exerbation of respiratory inflammation, causes damage-repair process constantly to carry out, and inflammatory damage-reparation causes cicatrization repeatedly, causes airway constriction to cause airway resistance to increase further, decline in pulmonary function occurs, even result in COPD.Lung function can evaluate the degree of flow limitation preferably.RLMax, ReMax, RLArea, ReArea are the good index of detection flow limitation and airway resistance, and PEF can react small airway function preferably, and small airway flow limitation sensitivity is higher.Carry out animal lung function test to contribute to evaluating PM2.5 and cause the air flue changes of function situation of model of lung injury mice, the inspiratory resistance of this experimental studies results display model matched group and expiratory resistance are all significantly greater than blank group (P < 0.05), and air flue compliance and firmly maximum expiratory flow rate relatively blank group are decreased obviously (P < 0.05)；Sample 1.～inspiratory resistance 5. organized and expiratory resistance be all significantly less than model control group (P < 0.05), air flue comply with domestic animal and firmly maximum expiratory flow rate relatively model control group rise (P < 0.05)；Inspiratory resistance that 6. and 7. sample is organized and expiratory resistance and model control group without significant difference (P > 0.05), air flue comply with domestic animal and firmly maximum expiratory flow rate and model control group without significant difference (P > 0.05).Result is shown in following table:
RLMax ReMax CdynMax RLArea ReArea CdynArea PEF
Blank group 9.25 7.75 20.38 9.13 8.25 16.00 19.50
Model control group 19.75 19.50 4.50 18.50 16.38 7.38 5.75
Sample is group 1. 8.50 8.25 19.27 9.54 9.76 15.83 18.78
Sample is group 2. 8.76 8.43 19.03 9.78 9.88 15.35 18.49
Sample is group 3. 8.83 8.39 19.09 9.74 9.86 15.46 18.53
Sample is group 4. 8.92 8.54 19.12 9.69 9.89 15.39 18.57
Sample is group 5. 8.79 8.56 19.17 9.67 9.83 15.55 18.62
Sample is group 6. 19.24 19.12 4.76 18.09 16.15 7.46 5.81
Sample is group 7. 19.32 19.17 4.82 18.16 16.13 7.49 5.87
It is shown that PM2.5 causes injury of lung animal model mice, to there is ventilatory function limited, and the especially dysfunction of small airway, airway resistance substantially increases, and there is the probability of airway obstruction；After anti-haze beverage provided by the invention is treated, the pulmonary function of mice improves, and main manifestations is the increase of the reduction of airway resistance, air flue compliance；PM2.5 is caused the therapeutic effect of injury of lung and is likely to relevant with the weight ratio of Effect raw material Semen Raphani and Semen stizolobii capitati by the present invention anti-haze beverage.
The effect of above-described embodiment indicates that the essentiality content of the present invention, but does not limit protection scope of the present invention with this.It will be understood by those within the art that, it is possible to technical scheme is modified or equivalent replacement, without deviating from essence and the protection domain of technical solution of the present invention.

Claims (9)

1. reduce PM2.5 and cause an anti-haze beverage for injury of lung, including Effect raw material and food additive, it is characterised in that described Effect raw material includes: Flos Lonicerae, 5～15 parts；Radix Platycodonis, 10～20 parts；Fructus Momordicae, 15～25 parts；Herba Menthae, 4～8 parts；Caulis Momordicae Charantiae, 2～4 parts；Fructus Jujubae, 10～20 parts；Fructus Lycii, 10～20 parts；Radix Codonopsis, 6～10 parts；Semen Raphani and Semen stizolobii capitati totally 2～4 parts；Wherein, the weight ratio of described Semen Raphani and Semen stizolobii capitati is 1～3:1.
2. the PM2.5 that reduces according to claim 1 causes the anti-haze beverage of injury of lung, including Effect raw material and food additive, it is characterised in that described Effect raw material includes: Flos Lonicerae, and 10 parts；Radix Platycodonis, 15 parts；Fructus Momordicae, 20 parts；Herba Menthae, 6 parts；Caulis Momordicae Charantiae, 3 parts；Fructus Jujubae, 15 parts；Fructus Lycii, 15 parts；Radix Codonopsis, 8 parts；Semen Raphani and Semen stizolobii capitati totally 3 parts；Wherein, the weight ratio of described Semen Raphani and Semen stizolobii capitati is 2:1.
3. the PM2.5 that reduces according to claim 1 causes the anti-haze beverage of injury of lung, including Effect raw material and food additive, it is characterised in that described Effect raw material includes: Flos Lonicerae, and 5 parts；Radix Platycodonis, 10 parts；Fructus Momordicae, 15 parts；Herba Menthae, 4 parts；Caulis Momordicae Charantiae, 2 parts；Fructus Jujubae, 10 parts；Fructus Lycii, 10 parts；Radix Codonopsis, 6 parts；Semen Raphani and Semen stizolobii capitati totally 2 parts；Wherein, the weight ratio of described Semen Raphani and Semen stizolobii capitati is 1:1.
4. the PM2.5 that reduces according to claim 1 causes the anti-haze beverage of injury of lung, including Effect raw material and food additive, it is characterised in that described Effect raw material includes: Flos Lonicerae, and 15 parts；Radix Platycodonis, 20 parts；Fructus Momordicae, 25 parts；Herba Menthae, 8 parts；Caulis Momordicae Charantiae, 4 parts；Fructus Jujubae, 20 parts；Fructus Lycii, 20 parts；Radix Codonopsis, 10 parts；Semen Raphani and Semen stizolobii capitati totally 4 parts；Wherein, the weight ratio of described Semen Raphani and Semen stizolobii capitati is 3:1.
5. cause the anti-haze beverage of injury of lung according to the arbitrary described PM2.5 that reduces of Claims 1 to 4, it is characterised in that: described food additive includes sweeting agent and other acceptable food additive.
6. the PM2.5 that reduces according to claim 5 causes the anti-haze beverage of injury of lung, it is characterised in that: described sweeting agent is saccharide and/or non-sugar sweetener.
7. the PM2.5 that reduces according to claim 6 causes the anti-haze beverage of injury of lung, it is characterised in that: described sugar sweetener is selected from one or more in white sugar, brown sugar, brown sugar, nigecose, high fructose syrup, sucrose, fructose, glucose, maltose, Mel.
8. the PM2.5 that reduces according to claim 6 causes the anti-haze beverage of injury of lung, it is characterised in that: described non-sugar sweetener is selected from one or more in cyclamate, acesulfame potassium, sorbitol, xylitol, maltose alcohol, glycyrrhizin, stevioside, aspartame, sucralose.
9. the preparation method of the arbitrary described anti-haze beverage of Claims 1 to 4, it is characterised in that comprise the steps:
Step S1, cleans above-mentioned Effect raw material, dries, add 15～25 times amount pure water, decoct 90～120 minutes, filter, obtain filtrate；Filtering residue adds 15～25 times amount pure water heating extraction 60～90 minutes again, filters, obtains filtrate；Twice filtrate merging is concentrated into every 25ml extracting solution containing Effect raw material 1g；
Step S2, dilutes extracting solution pure water, adds food additive, dissolve, boil, sterilizing, filter, subpackage.
CN201610164630.1A 2016-03-22 2016-03-22 Anti-haze drink capable of reducing haze-induced lung injuries and preparation method of anti-haze drink Withdrawn CN105747047A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201610164630.1A CN105747047A (en) 2016-03-22 2016-03-22 Anti-haze drink capable of reducing haze-induced lung injuries and preparation method of anti-haze drink

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201610164630.1A CN105747047A (en) 2016-03-22 2016-03-22 Anti-haze drink capable of reducing haze-induced lung injuries and preparation method of anti-haze drink

Publications (1)

Publication Number Publication Date
CN105747047A true CN105747047A (en) 2016-07-13

Family

ID=56346131

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201610164630.1A Withdrawn CN105747047A (en) 2016-03-22 2016-03-22 Anti-haze drink capable of reducing haze-induced lung injuries and preparation method of anti-haze drink

Country Status (1)

Country Link
CN (1) CN105747047A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106667973A (en) * 2017-01-22 2017-05-17 西咸新区世云工研坊生物科技有限公司 Composition for preventing haze damage and preparation method and spray of composition
CN107279391A (en) * 2017-07-18 2017-10-24 佛山瑞匀医疗器械有限公司 Moistening throat and clearing lung type tea and its manufacture method
CN108404011A (en) * 2018-05-10 2018-08-17 王芬 A kind of haze removing heat from the lung to relieve cough Chinese medicine composition and preparation method thereof

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103493921A (en) * 2013-09-18 2014-01-08 朱红 Throat clearing and moistening healthcare tea
US20140220163A1 (en) * 2014-02-04 2014-08-07 Hamzeh Soleimani Babadi Powder mixture composition of natural materials for controlling deficiencies in polypeptide alpha and beta hemoglobin chains
CN104026691A (en) * 2014-06-25 2014-09-10 广州白云山潘高寿药业股份有限公司 Anti-haze drink and preparation method of anti-haze drink
CN104306908A (en) * 2014-03-24 2015-01-28 河南茗轩食品科技有限公司 Health-care composition for preventing respiratory system diseases caused by haze, and preparation method thereof
CN105360522A (en) * 2015-12-03 2016-03-02 彭楚铭 Special health-care tea capable of clearing lung, for fog and haze

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103493921A (en) * 2013-09-18 2014-01-08 朱红 Throat clearing and moistening healthcare tea
US20140220163A1 (en) * 2014-02-04 2014-08-07 Hamzeh Soleimani Babadi Powder mixture composition of natural materials for controlling deficiencies in polypeptide alpha and beta hemoglobin chains
CN104306908A (en) * 2014-03-24 2015-01-28 河南茗轩食品科技有限公司 Health-care composition for preventing respiratory system diseases caused by haze, and preparation method thereof
CN104026691A (en) * 2014-06-25 2014-09-10 广州白云山潘高寿药业股份有限公司 Anti-haze drink and preparation method of anti-haze drink
CN105360522A (en) * 2015-12-03 2016-03-02 彭楚铭 Special health-care tea capable of clearing lung, for fog and haze

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
南京中医药大学 编著: "《中药大辞典 下册》", 31 March 2006, 上海科学技术出版社 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN106667973A (en) * 2017-01-22 2017-05-17 西咸新区世云工研坊生物科技有限公司 Composition for preventing haze damage and preparation method and spray of composition
CN107279391A (en) * 2017-07-18 2017-10-24 佛山瑞匀医疗器械有限公司 Moistening throat and clearing lung type tea and its manufacture method
CN108404011A (en) * 2018-05-10 2018-08-17 王芬 A kind of haze removing heat from the lung to relieve cough Chinese medicine composition and preparation method thereof

Similar Documents

Publication Publication Date Title
CN105105235B (en) Anti-fog haze drink and preparation method thereof
CN103127369A (en) Traditional Chinese medicine composition for treating respiratory tract haze disoperation and preparation method
CN103120703A (en) Care solution for cleaning nasal cavity and related composition
CN105747047A (en) Anti-haze drink capable of reducing haze-induced lung injuries and preparation method of anti-haze drink
CN106421039A (en) Traditional Chinese medicine composition for preventing and treating haze-caused diseases, preparation method thereof and mask containing traditional Chinese medicine
CN105360524A (en) Fog and haze lung-clearing tea
CN105998280A (en) Anti-haze medical and edible dual-purpose cordyceps militaris and snow lotus herb composition and preparation method and application thereof
CN102626482B (en) Application of Chinese medicinal composition to preparation of medicament for treating and improving lung PM2.5 deposition
CN105135520A (en) Air water making, humidifying and purifying integrated device and control method thereof
CN112587595B (en) Traditional Chinese medicine composition for clearing heat and relieving exterior syndrome and preparation method thereof
CN105327104A (en) Haze lung clearing drink
CN1965907B (en) Chinese medicinal spray for preventing and treating cold and method for preparing same
CN105726920B (en) A kind of anti-PM2.5 chewable tablets and preparation method thereof
CN104887944A (en) Biological agent with function of preventing and treating respiratory diseases caused by haze and preparation method of biological agent
CN105747065A (en) Beverage with throat-clearing and lung-clearing functions
CN106692504B (en) Health food with effects of clearing heat from throat, relieving cough, moistening lung and preventing haze and preparation method of health food
CN105381193A (en) Haze-resistant lung-clearing lozenge
CN104491121A (en) Anti-bacterial essence solution for treating respiratory diseases and preparation method of anti-bacterial essence solution
CN107129869A (en) Smooth breathing essential oil and preparation method and application
CN1589852A (en) External use nose drop agent for treating common cold and its preparation method
CN102488831B (en) Medicine for treating respiratory diseases and preparation method thereof
CN107714920A (en) A kind of Chinese medicine composition with pharynx-clearing throat-benefiting antitussive action and preparation method thereof
CN109550017A (en) The hot anti-inflammatory preventing phlegm from forming and stopping coughing Chinese medicine preparation of clearing lung-heat
CN109106774A (en) A kind of Chinese medicine composition and its preparation method and application for treating adenoids and its complication
CN109601878A (en) For protecting the nutraceutical of injury of lungs

Legal Events

Date Code Title Description
C06 Publication
PB01 Publication
C10 Entry into substantive examination
SE01 Entry into force of request for substantive examination
WW01 Invention patent application withdrawn after publication

Application publication date: 20160713

WW01 Invention patent application withdrawn after publication