CN105734101A - Method for preparing poria cocos peptide - Google Patents
Method for preparing poria cocos peptide Download PDFInfo
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- CN105734101A CN105734101A CN201610218529.XA CN201610218529A CN105734101A CN 105734101 A CN105734101 A CN 105734101A CN 201610218529 A CN201610218529 A CN 201610218529A CN 105734101 A CN105734101 A CN 105734101A
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- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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Abstract
A method for preparing poria cocos peptide includes the following steps that firstly, fresh poria cocos is harvested, cleaned, diced and conveyed into a low-temperature cold air drying device to be dried; secondly, the dried poria cocos dices are conveyed into a rubbing machine to be rubbed continuously for 20 min; thirdly, the poria cocos dices are conveyed into a tumbling machine to be tumbled; fourthly, the tumbled poria cocos dices are subjected to superfine smashing and screened by a 100-400-mesh sifter, the poria cocos dices are frozen for 40 min in the temperature of minus 15 DEG C, the poria cocos dices are taken out, water is added for stirring, and a wet material is obtained; fifthly, the wet material is conveyed into a microwave processing device for interval microwave processing; sixthly, the wet material subjected to microwave processing is soaked for 1.5 h in water bath at the temperature of 75 DEG C, papain is added for stirring, extraction is conducted for 1-4 h at the temperature of 40-60 DEG C, and an extraction solution is obtained; seventhly, vacuum suction filtration is conducted by means of a circulating water type multi-purpose vacuum pump to obtain a peptide solution subjected to suction filtration; eighthly, the raw material peptide solution is subjected to vacuum spraying and drying, and dried matter is collected as the poria cocos peptide.
Description
Technical field
The present invention relates to technical field of bioengineering, the preparation method being specifically related to a kind of Poria peptide.
Background technology
Poria is the dry sclerotia of On Polyporaceae, as the medicinal health product history of existing more than 2000 year.For the big kind of Chinese medicine of medicine-food two-purpose, recording in Shennong's Herbal, version over the years " Chinese Pharmacopoeia " is all recorded.Theory of Chinese medical science thinks that it has effect of promoting diuresis to eliminate damp pathogen, spleen invigorating invigorating middle warmer, mind tranquilizing and the heart calming, can treat the diseases such as dysuria, edema distension, cough due to fluid-retention, insufficiency of the spleen few food, loose stool and diarrhea, palpitation and insomnia and dizziness；Modern pharmacological research shows, Poria extract has the pharmacological actions such as enhancing immunologic function, mutation, defying age, antiallergic, suppression tumor growth.The main active substance of Poria extract is triterpene, polysaccharide, fatty acid, sterol etc..Wherein pachyman there is antitumor, antiviral, antioxidation, enhancing human body immunity power, protect the liver, hypnosis, the effect such as antiinflammatory, can be widely applied to the field such as health care, food.Triterpene substance has immunomodulating, antitumor, antiinflammatory, induction differentiation Leukemia Cell Lines HL-60 etc. and has good pharmacologically active.
Poria is one of Chinese herbal medicine of medicine-food two-purpose, has antiinflammation, is currently used primarily in and is used as medicine with other drug matching, and its effect is poor, and consumption is big.Its active ingredient not being made what micromolecule polypeptide used as health food, it eats and medical value all ends are played well.
Summary of the invention
The technical problem to be solved is in that to provide a kind of high financial profit, the preparation method of the Poria peptide that end product quality is stable.
The technical problem to be solved realizes by the following technical solutions:
A kind of preparation method of Poria peptide, concrete grammar step is as follows:
(1) gather fresh Poria, stripping and slicing after cleaning, send in low-temperature cold wind drying equipment and carry out drying and processing, to moisture about 40%, dry temperature and control at 35-45 DEG C；
(2) the Poria block after drying being sent into kneading machine to carry out rubbing process, rub continuously 20 minutes, take out and carry out K cryogenic treatment under feeding low temperature environment, temperature controls between 0-5 DEG C, processes 60-80 minute；
(3) the Poria block after K cryogenic treatment is sent into tumbler and carry out tumbling process, during tumbling every 5-8 minute evacuation once, exhaust tumbling 5-10 minute after vacuum, then vacuum pumping, so circulation 2-4 time every time；
(4) the Poria block after tumbling being processed carries out micronizing, crosses 100～400 order medicines sieves, under subzero 15 DEG C of environment freezing 40 minutes, takes out the stirring that adds water, and to the water content of Indian Bread at about 40%-42%, obtains moistening expecting；
(5) being sent in microwave processing equipment by moistening material, interval microwave treatment under microwave frequency 2450MHz, power 500W, interval time is 5 minutes, each microwave treatment 10 minutes, continuously performs 3-4 time；
(6) the moistening material after microwave treatment is taken by weight 1 (moistening material): 12-18 (water) left and right adds water, soak 1.5 hours in 75 DEG C of water-baths, it is subsequently adding papain stir and maintain the temperature at 40～60 DEG C of lixiviates 1～4 hour, obtains extracting solution stand-by；Wherein the addition of papain is the 0.1-1% of moistening material weight；
(7) take said extracted liquid, utilize the multiplex vacuum pump of circulating water type to carry out vacuum filtration, obtain the peptide liquid after sucking filtration；
(8) raw material peptide liquid is carried out vacuum spray drying, collect dried object and be Poria peptide.
Poria peptides products of the present invention takes proof through 142 the examination person's of clothes clinics, there is significant anti-inflammation health care effect, especially to various gynecological inflammations, such as pelvic inflammatory disease, adnexa is light, vaginitis, cervicitis and cyclomastopathy, mastitis etc. have more prominent improvement and therapeutic effect, and it is without any side effects, its good health care effect, it is not added with Western medicine, long-term taking is addiction not, there is effect height, human absorptivity is high, make functional component fast onset effect, definite effect, use easy to carry, method is simply unique, resource is wide, substantial amounts of drug resource can be saved, cost is low, the outstanding features such as application easy to spread.Its method for making uniqueness is simple and practical.Tuckahoe polypeptide can medicine-food two-purpose, it is particularly possible to carry out extensive use in the goods such as food, health food or medicine.
The invention has the beneficial effects as follows: the present invention is convenient and swift, extraction efficiency is high, technical process is controlled, the constant product quality of processing, and this technology passes through biological enzymolysis technology, Poria is become water-soluble active component small-molecular peptides by after biological enzyme hydrolysis, and absorption of human body is completely and rapidly.
Detailed description of the invention
For the technological means making the present invention realize, creation characteristic, reach purpose and effect and be easy to understand, below in conjunction with specific embodiment, the present invention is expanded on further.
A kind of preparation method of Poria peptide, concrete grammar step is as follows:
(1) gather fresh Poria, stripping and slicing after cleaning, send in low-temperature cold wind drying equipment and carry out drying and processing, to moisture about 40%, dry temperature and control at 35-45 DEG C；
(2) the Poria block after drying being sent into kneading machine to carry out rubbing process, rub continuously 20 minutes, take out and carry out K cryogenic treatment under feeding low temperature environment, temperature controls between 0-5 DEG C, processes 60-80 minute；
(3) the Poria block after K cryogenic treatment is sent into tumbler and carry out tumbling process, during tumbling every 5-8 minute evacuation once, exhaust tumbling 5-10 minute after vacuum, then vacuum pumping, so circulation 2-4 time every time；
(4) the Poria block after tumbling being processed carries out micronizing, crosses 100～400 order medicines sieves, under subzero 15 DEG C of environment freezing 40 minutes, takes out the stirring that adds water, and to the water content of Indian Bread at about 40%-42%, obtains moistening expecting；
(5) being sent in microwave processing equipment by moistening material, interval microwave treatment under microwave frequency 2450MHz, power 500W, interval time is 5 minutes, each microwave treatment 10 minutes, continuously performs 3-4 time；
(6) the moistening material after microwave treatment is taken by weight 1 (moistening material): 12-18 (water) left and right adds water, soak 1.5 hours in 75 DEG C of water-baths, it is subsequently adding papain stir and maintain the temperature at 40～60 DEG C of lixiviates 1～4 hour, obtains extracting solution stand-by；Wherein the addition of papain is the 0.1-1% of moistening material weight；
(7) take said extracted liquid, utilize the multiplex vacuum pump of circulating water type to carry out vacuum filtration, obtain the peptide liquid after sucking filtration；
(8) raw material peptide liquid is carried out vacuum spray drying, collect dried object and be Poria peptide.
The ultimate principle of the present invention and principal character and advantages of the present invention have more than been shown and described.Skilled person will appreciate that of the industry; the present invention is not restricted to the described embodiments; described in above-described embodiment and description is that principles of the invention is described; without departing from the spirit and scope of the present invention; the present invention also has various changes and modifications, and these changes and improvements both fall within the claimed scope of the invention.Claimed scope is defined by appending claims and equivalent thereof.
Claims (1)
1. the preparation method of a Poria peptide, it is characterised in that concrete grammar step is as follows:
(1) gather fresh Poria, stripping and slicing after cleaning, send in low-temperature cold wind drying equipment and carry out drying and processing, to moisture about 40%, dry temperature and control at 35-45 DEG C；
(2) the Poria block after drying being sent into kneading machine to carry out rubbing process, rub continuously 20 minutes, take out and carry out K cryogenic treatment under feeding low temperature environment, temperature controls between 0-5 DEG C, processes 60-80 minute；
(3) the Poria block after K cryogenic treatment is sent into tumbler and carry out tumbling process, during tumbling every 5-8 minute evacuation once, exhaust tumbling 5-10 minute after vacuum, then vacuum pumping, so circulation 2-4 time every time；
(4) the Poria block after tumbling being processed carries out micronizing, crosses 100～400 order medicines sieves, under subzero 15 DEG C of environment freezing 40 minutes, takes out the stirring that adds water, and to the water content of Indian Bread at 40%-42%, obtains moistening expecting；
(5) being sent in microwave processing equipment by moistening material, interval microwave treatment under microwave frequency 2450MHz, power 500W, interval time is 5 minutes, each microwave treatment 10 minutes, continuously performs 3-4 time；
(6) take the moistening material after microwave treatment to add water by weight 1:12-18, soak 1.5 hours in 75 DEG C of water-baths, be subsequently adding papain and stir and maintain the temperature at 40～60 DEG C of lixiviates 1～4 hour, obtain extracting solution stand-by；Wherein the addition of papain is the 0.1-1% of moistening material weight；
(7) take said extracted liquid, utilize the multiplex vacuum pump of circulating water type to carry out vacuum filtration, obtain the peptide liquid after sucking filtration；
(8) raw material peptide liquid is carried out vacuum spray drying, collect dried object and be Poria peptide.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610218529.XA CN105734101A (en) | 2016-04-07 | 2016-04-07 | Method for preparing poria cocos peptide |
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| CN201610218529.XA CN105734101A (en) | 2016-04-07 | 2016-04-07 | Method for preparing poria cocos peptide |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106259171A (en) * | 2016-08-10 | 2017-01-04 | 金寨县万紫千红农业科技开发有限公司 | The method utilizing tunnel cultivation high reproductive rate kind rabbit |
| CN108315379A (en) * | 2018-04-27 | 2018-07-24 | 怀化学院 | A kind of enzyme solution of tuckahoe polypeptide |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101933610A (en) * | 2010-07-23 | 2011-01-05 | 江苏安惠生物科技有限公司 | Edible and medical fungus cell component comprehensive extraction method and use of extract |
| CN104758321A (en) * | 2015-03-18 | 2015-07-08 | 金寨金芝源生物工程有限公司 | Ultralow-temperature physical wall-breaking method of ganoderma lucidum spore powder |
-
2016
- 2016-04-07 CN CN201610218529.XA patent/CN105734101A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101933610A (en) * | 2010-07-23 | 2011-01-05 | 江苏安惠生物科技有限公司 | Edible and medical fungus cell component comprehensive extraction method and use of extract |
| CN104758321A (en) * | 2015-03-18 | 2015-07-08 | 金寨金芝源生物工程有限公司 | Ultralow-temperature physical wall-breaking method of ganoderma lucidum spore powder |
Non-Patent Citations (2)
| Title |
|---|
| 张晓娟等: "微波辅助酶法提取茯苓中茯苓多糖的工艺研究", 《安徽农业科学》 * |
| 李建芝等: "探究木瓜蛋白酶在中药加工中的应用", 《求医问药》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106259171A (en) * | 2016-08-10 | 2017-01-04 | 金寨县万紫千红农业科技开发有限公司 | The method utilizing tunnel cultivation high reproductive rate kind rabbit |
| CN108315379A (en) * | 2018-04-27 | 2018-07-24 | 怀化学院 | A kind of enzyme solution of tuckahoe polypeptide |
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