CN105724294B - A method of prevent fish-egg from suffering from saprolegniasis - Google Patents
A method of prevent fish-egg from suffering from saprolegniasis Download PDFInfo
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- CN105724294B CN105724294B CN201610127894.XA CN201610127894A CN105724294B CN 105724294 B CN105724294 B CN 105724294B CN 201610127894 A CN201610127894 A CN 201610127894A CN 105724294 B CN105724294 B CN 105724294B
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- fish
- egg
- pool
- saprolegniasis
- water
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- 238000000034 method Methods 0.000 title claims abstract description 47
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 68
- OSVXSBDYLRYLIG-UHFFFAOYSA-N dioxidochlorine(.) Chemical compound O=Cl=O OSVXSBDYLRYLIG-UHFFFAOYSA-N 0.000 claims abstract description 36
- 241000251468 Actinopterygii Species 0.000 claims abstract description 27
- 239000004155 Chlorine dioxide Substances 0.000 claims abstract description 18
- 235000019398 chlorine dioxide Nutrition 0.000 claims abstract description 18
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims abstract description 16
- RJMUSRYZPJIFPJ-UHFFFAOYSA-N niclosamide Chemical compound OC1=CC=C(Cl)C=C1C(=O)NC1=CC=C([N+]([O-])=O)C=C1Cl RJMUSRYZPJIFPJ-UHFFFAOYSA-N 0.000 claims abstract description 13
- 230000012447 hatching Effects 0.000 claims abstract description 12
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 7
- 238000011017 operating method Methods 0.000 claims abstract description 3
- 230000027272 reproductive process Effects 0.000 claims description 11
- 230000004720 fertilization Effects 0.000 claims description 10
- 230000017448 oviposition Effects 0.000 claims description 8
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 claims description 7
- 229960000907 methylthioninium chloride Drugs 0.000 claims description 7
- 238000012545 processing Methods 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 4
- 201000010099 disease Diseases 0.000 claims description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims description 4
- 238000011534 incubation Methods 0.000 claims description 4
- 238000006213 oxygenation reaction Methods 0.000 claims description 3
- 239000011148 porous material Substances 0.000 claims description 3
- 239000006041 probiotic Substances 0.000 claims description 3
- 235000018291 probiotics Nutrition 0.000 claims description 3
- 230000000243 photosynthetic effect Effects 0.000 claims description 2
- 241001669656 Odontobutis obscura Species 0.000 abstract description 6
- 238000009395 breeding Methods 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 4
- 244000052769 pathogen Species 0.000 abstract description 2
- 230000001717 pathogenic effect Effects 0.000 abstract description 2
- 241001125889 Micropterus salmoides Species 0.000 abstract 1
- 239000000853 adhesive Substances 0.000 abstract 1
- 230000001070 adhesive effect Effects 0.000 abstract 1
- 102000002322 Egg Proteins Human genes 0.000 description 6
- 108010000912 Egg Proteins Proteins 0.000 description 6
- 210000004681 ovum Anatomy 0.000 description 6
- 229940079593 drug Drugs 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 241000565675 Oncomelania Species 0.000 description 3
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 description 2
- 241000124033 Salix Species 0.000 description 2
- 150000001412 amines Chemical class 0.000 description 2
- 239000000460 chlorine Substances 0.000 description 2
- 229910052801 chlorine Inorganic materials 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000013601 eggs Nutrition 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000269799 Perca fluviatilis Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000007598 dipping method Methods 0.000 description 1
- 239000013505 freshwater Substances 0.000 description 1
- 235000013372 meat Nutrition 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F9/00—Multistage treatment of water, waste water or sewage
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F1/00—Treatment of water, waste water, or sewage
- C02F1/50—Treatment of water, waste water, or sewage by addition or application of a germicide or by oligodynamic treatment
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2301/00—General aspects of water treatment
- C02F2301/08—Multistage treatments, e.g. repetition of the same process step under different conditions
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2303/00—Specific treatment goals
- C02F2303/04—Disinfection
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
Abstract
The invention belongs to fish breeding fields, are specifically related to a kind of method for preventing fish-egg from suffering from saprolegniasis.The method for preventing fish-egg from suffering from saprolegniasis includes operating procedure: full water is first stored into pool；Then niclosamidum is put into the water body into the pool, the input amount of every meter of pool depth of water niclosamidum is controlled in 2-3kg per acre；Copper sulphate is put into water body after 7-10 days, then into the pool, the input amount of every meter of pool depth of water copper sulphate is controlled in 0.75-1.25kg per acre；It puts into chlorine dioxide after 4-5 days, in the water body into the pool to carry out disinfection, the input amount 150-200g of chlorine dioxide；After 2 days, above-mentioned water body is used for hatch fish roe process.The method of the invention can kill pathogen from water source, this method can be widely applied to during fish culture, the names spy fish such as the Odontobutis obscura grown especially for the Micropterus salmoides of production adhesive egg, hatch fish roe period, solve the problems, such as that saprolegniasis, hatching rate height occur in hatching process for fish-egg when carrying out hatch fish roe using treated water body.
Description
Technical field
The invention belongs to fish breeding field, it is specifically related to a kind of prevent the side that fish-egg suffers from saprolegniasis in reproductive process
Method.
Background technique
Water mo(u)ld is stored in extensively in fresh water or brackish water waters and slowly drained soil all over the world, on dead organic matter
It is saprophytic, for it is a kind of often mould.During fish breeding, fish-egg easily meets with the infection of water mo(u)ld and must go up saprolegniasis.
Method that traditional control fish suffer from saprolegniasis is realized by control hatch fish roe temperature, but in early breeding stage, Wai Tangshui
Temperature is low, and temperature-rise period is more troublesome and effect is not ideal enough；And this method is not appropriate for the longer Odontobutis obscura fish of incubation cycle
Ovum.Odontobutis obscura is that a kind of individual is smaller, but the fish that meat is very delicious, and the hatch fish roe period of the fish is usually in 28-30
It, the longer incubation period causes the fish-egg of the fish not hatched also just to have infectd saprolegniasis, typically upon infecing water mold
Disease, fish-egg basic loss totally, to raiser cause very huge economic loss.
Summary of the invention
The method that fish-egg suffers from saprolegniasis in reproductive process is prevented in order to solve the above technical problem, the present invention provides a kind of,
This method can be widely applied to during fish culture, and especially for the Odontobutis obscura that the hatch fish roe period is long, this method is effective
Solve the problems, such as that saprolegniasis occurs in hatching process for fish-egg.
In order to achieve the object of the present invention, the invention adopts the following technical scheme:
A method of prevent fish-egg in reproductive process from suffering from saprolegniasis, including following operating procedure:
A, full water is first stored into pool；Then niclosamidum is put into the water body into the pool, per acre every meter of pool depth of water chlorine nitre
The input amount of willow amine is controlled in 2-3kg；
B, copper sulphate is put into the water body after 7-10 days, then into the pool, per acre the input amount control of every meter of pool depth of water copper sulphate
System is in 0.75-1.25kg；
C, it puts into chlorine dioxide after 4-5 days, in the water body into the pool to carry out disinfection, the input amount 150- of chlorine dioxide
200g；
D, after 2 days, above-mentioned water body is used for hatch fish roe process.
Further technical solution, the input amount of niclosamidum is 2.5kg in the step a.
Further technical solution, the input amount of copper sulphate is 1kg in the step b.
Further technical solution, the input amount of chlorine dioxide is 180g in the step c.
Further technical solution, water body described in the step d are also used to parent culture, oviposition process, the parent
Cultivation, oviposition process carry out in parent culture pond and spawning pond respectively, are mounted in the parent culture pond and spawning pond
Micro-pore oxygenation equipment, the parent culture, oviposition process, which respectively further comprise, provides sufficient bait for parent population, in addition uses micro- life
State preparation adjusts the water quality in the parent culture pond and spawning pond, and the probiotics are photosynthetic bacteria or EM bacterium.
Further technical solution takes following operation if saprolegniasis occurs for the later period of the reproductive process:
(1) the fish-egg batch for rate of fertilization lower than 85% is carried out eliminating processing and/or is put into independent incubation equipment；
(2) it is higher than 85% fish-egg batch for rate of fertilization, chooses and carry out fish-egg again after unfertilized and mouldy fish-egg and incubate
Change process；
It is further suppressed while carrying out aforesaid operations (1) or (2) by 24-26 DEG C of temperature control or methylene blue solution of splashing
The generation of saprolegniasis, in order to avoid influence the hatching process of normal fish-egg.
The beneficial effects of the present invention are:
(1) the method for the invention can kill pathogen from water source, and using treated, water body carries out hatch fish roe
Process, hatching rate is high, solves the problems, such as that saprolegniasis occurs in hatching process for fish-egg.The niclosamidum can kill
Oncomelania in the pool, since oncomelania is the main boarding body of germ, worm's ovum, germ, worm's ovum can effectively be prevented to fish-egg by killing oncomelania
It is influenced caused by hatching；The copper sulphate is further handled water body, and copper sulphate can kill algae with desinsection；The chlorine dioxide
Disinfection treatment is further carried out to water body.Above-mentioned processing step is indispensable, and treated that water body is not only suitable for for this method
Hatch fish roe has also prevented the generation for the case where fish-egg infects saprolegniasis completely.
(2) present invention is carried out the input amount and water body of drug corresponding in step a, b, c by the processing time of drug
Stringent control, the input amount of drug, the processing time meet water body processing reach prevent saprolegniasis from occurring under the premise of,
Avoid the negative effect that drug may excessively generate water quality.
(3) cooperation micro-pore oxygenation equipment and sufficient bait can be further during parent culture, oviposition in the present invention
The good development for ensureing parent is conducive to the rate of fertilization for improving fish-egg in this way.
It (4), can be by eliminating the low fish of rate of fertilization if heretofore described post-reproductive fish-egg infects saprolegniasis accidentally
Ovum batch, and choose unfertilized and mildew fish-egg from the high fish-egg batch of rate of fertilization, to guarantee incubating for normal fertilization ovum
Change process cooperates temperature control or suitable methylene blue solution of splashing that can be the ability to easily control saprolegniasis.
Specific embodiment
Embodiment 1
A, full water is first stored into pool；Then niclosamidum is put into the water body into the pool, per acre every meter of pool depth of water chlorine nitre
The input amount of willow amine is controlled in 2kg；
B, copper sulphate is put into the water body after 7 days, then into the pool, the input amount control of every meter of pool depth of water copper sulphate exists per acre
0.75kg；
C, after 4 days, chlorine dioxide is finally put into the water body into the pool and is carried out disinfection, chlorine dioxide input amount at high temperature
200g；
D, after 2 days, chlorine dioxide failure carries out Odontobutis obscura hatch fish roe process using above-mentioned water body.
Embodiment 2
A, niclosamidum is put into the water body into the pool, the input amount of every meter of pool depth of water niclosamidum is controlled in 3kg per acre；
B, copper sulphate is put into the water body after 10 days, then into the pool, per acre the input amount control of every meter of pool depth of water copper sulphate
In 1.25kg；
C, after 5 days, chlorine dioxide is finally put into the water body into the pool and is carried out disinfection, chlorine dioxide input amount at high temperature
150g；
D, after 2 days, chlorine dioxide failure carries out Odontobutis obscura hatch fish roe using above-mentioned water body.
Embodiment 3
A, niclosamidum is put into the water body into the pool, the input amount control of every meter of pool depth of water niclosamidum exists per acre
2.5kg；
B, copper sulphate is put into the water body after 8 days, then into the pool, the input amount control of every meter of pool depth of water copper sulphate exists per acre
1kg；
C, after 5 days, chlorine dioxide is finally put into the water body into the pool and is carried out disinfection, the input amount 180g of chlorine dioxide；
D, after 2 days, chlorine dioxide failure carries out perch hatch fish roe process using above-mentioned water body.
For water body in above-described embodiment after treatment in the reproductive process for fish, fish-egg does not occur infection water mold
The situation of disease, and the hatching rate of fish-egg is high.
Embodiment 4
Grouping 1: exogenous saprolegniasis pathogeny is added the hatching process of treated water body the is used for fish-egg of embodiment 1
Through in treated the water body of above-described embodiment 1；
Grouping 2: exogenous saprolegniasis pathogeny is added the hatching process of treated water body the is used for fish-egg of embodiment 2
Through in treated the water body of above-described embodiment 2；
Grouping 3: exogenous saprolegniasis pathogeny is added the hatching process of treated water body the is used for fish-egg of embodiment 3
Through in treated the water body of above-described embodiment 3；
Saprolegniasis takes place in fish-egg in above-mentioned grouping 1,2,3, is lower than 85% fish to rate of fertilization in grouping 2,3 at this time
Ovum batch carries out eliminating processing, and 85% fish-egg batch is higher than to rate of fertilization, is carried out again after choosing unfertilized and mouldy fish-egg
Incubating oosperm process；Methylene blue solution is sprayed on fertilized eggs simultaneously, methylene blue includes dipping method and two kinds of full pool spilling head
Mode: its Chinese herb bath method is that fertilized eggs are put into the methylene blue solution of 10mg/L, is taken out after impregnating 10-20 minutes；Quan Chi
Methylene blue concentration is 2-3mg/L when splashing.
Above-mentioned test result is water mold in the saprolegniasis grouping 1 that is well controlled, and does not process being grouped in 2,3
Disease is further aggravated.
Claims (6)
1. a kind of prevent the method that fish-egg suffers from saprolegniasis in reproductive process, including following operating procedure:
A, full water is first stored into pool；Then niclosamidum is put into the water body into the pool, per acre every meter of pool depth of water niclosamidum
Input amount control in 2-3kg；
B, copper sulphate is put into the water body after 7-10 days, then into the pool, the input amount control of every meter of pool depth of water copper sulphate exists per acre
0.75-1.25kg；
C, it puts into chlorine dioxide after 4-5 days, in the water body into the pool to carry out disinfection, the input amount 150-200g of chlorine dioxide；
D, after 2 days, above-mentioned water body is used for hatch fish roe process.
2. preventing the method that fish-egg suffers from saprolegniasis in reproductive process as described in claim 1, it is characterised in that: the step a
The input amount of middle niclosamidum is 2.5kg.
3. preventing the method that fish-egg suffers from saprolegniasis in reproductive process as described in claim 1, it is characterised in that: the step b
The input amount of middle copper sulphate is 1kg.
4. preventing the method that fish-egg suffers from saprolegniasis in reproductive process as described in claim 1, it is characterised in that: the step c
The input amount of middle chlorine dioxide is 180g.
5. preventing the method that fish-egg suffers from saprolegniasis in reproductive process as described in claim 1, it is characterised in that: the step d
Described in water body be also used to parent culture, oviposition process, the parent culture, oviposition process are respectively in parent culture pond and oviposition
It is carried out in pond, micro-pore oxygenation equipment is mounted in the parent culture pond and spawning pond, the parent culture, oviposition process are also
It respectively includes providing sufficient bait for parent population, in addition be adjusted in the parent culture pond and spawning pond using probiotics
Water quality, the probiotics are photosynthetic bacteria or EM bacterium.
6. preventing the method that fish-egg suffers from saprolegniasis in reproductive process as claimed in claim 5, it is characterised in that: described to breed
If saprolegniasis occurs for the later period of journey, following operation is taken:
(1) the fish-egg batch for rate of fertilization lower than 85% is carried out eliminating processing and/or is put into independent incubation equipment；
(2) it is higher than 85% fish-egg batch for rate of fertilization, carries out hatch fish roe mistake again after choosing unfertilized and mouldy fish-egg
Journey；
Water mold is further suppressed by 24-26 DEG C of temperature control or methylene blue solution of splashing while carrying out aforesaid operations (1) or (2)
The generation of disease, in order to avoid influence the hatching process of normal fish-egg.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610127894.XA CN105724294B (en) | 2016-03-07 | 2016-03-07 | A method of prevent fish-egg from suffering from saprolegniasis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610127894.XA CN105724294B (en) | 2016-03-07 | 2016-03-07 | A method of prevent fish-egg from suffering from saprolegniasis |
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| Publication Number | Publication Date |
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| CN105724294A CN105724294A (en) | 2016-07-06 |
| CN105724294B true CN105724294B (en) | 2018-12-21 |
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| CN201610127894.XA Expired - Fee Related CN105724294B (en) | 2016-03-07 | 2016-03-07 | A method of prevent fish-egg from suffering from saprolegniasis |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107683798A (en) * | 2017-09-27 | 2018-02-13 | 清远职业技术学院 | A kind of Pelteobagrus fulvidraco artificial breeding and the method for raising |
| CN108812462A (en) * | 2018-06-26 | 2018-11-16 | 徐州市若水锦鲤养殖有限公司 | A kind of control method of fishpond insect pest |
| CN109197701B (en) * | 2018-10-22 | 2023-06-30 | 广州市建波鱼苗场有限公司 | Disinfection method capable of remarkably improving hatching rate of crucian eggs |
| CN110100766A (en) * | 2019-04-19 | 2019-08-09 | 山东科合海洋高技术有限公司 | A kind of hatching apparatus and method of the excellent fertilized eggs of round spot halibut |
| CN114223586A (en) * | 2021-12-01 | 2022-03-25 | 天津市金水族水产养殖有限公司 | Prevention and treatment method for fish egg saprolegniasis |
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| CN102948382A (en) * | 2012-11-13 | 2013-03-06 | 南京市水产科学研究所 | Method for controlling saprolegniasis of odontobutis obscura germ cells in incubation period |
| CN103392647A (en) * | 2013-07-23 | 2013-11-20 | 苏州市阳澄湖现代农业产业园特种水产养殖有限公司 | Method for scientifically and precisely breeding rice field eels |
| CN103975874B (en) * | 2014-04-21 | 2016-02-10 | 江苏湖丰特种水产品科技有限公司 | A kind of net cage ecological algae phase intensive culture method of Odontobutis obscura |
| JP5711846B1 (en) * | 2014-10-21 | 2015-05-07 | 助川化学株式会社 | Water mold control method in aquaculture water |
| CN104542402B (en) * | 2015-01-12 | 2017-04-05 | 陈治平 | A kind of ecological environment-friendly type Odontobulis mpotamophila industrialized cultivation method |
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